PMID- 29551135 OWN - NLM STAT- MEDLINE DCOM- 20181211 LR - 20200407 IS - 1557-8399 (Electronic) IS - 0065-3527 (Print) IS - 0065-3527 (Linking) VI - 100 DP - 2018 TI - Hosts and Sources of Endemic Human Coronaviruses. PG - 163-188 LID - S0065-3527(18)30001-0 [pii] LID - 10.1016/bs.aivir.2018.01.001 [doi] AB - The four endemic human coronaviruses HCoV-229E, -NL63, -OC43, and -HKU1 contribute a considerable share of upper and lower respiratory tract infections in adults and children. While their clinical representation resembles that of many other agents of the common cold, their evolutionary histories, and host associations could provide important insights into the natural history of past human pandemics. For two of these viruses, we have strong evidence suggesting an origin in major livestock species while primordial associations for all four viruses may have existed with bats and rodents. HCoV-NL63 and -229E may originate from bat reservoirs as assumed for many other coronaviruses, but HCoV-OC43 and -HKU1 seem more likely to have speciated from rodent-associated viruses. HCoV-OC43 is thought to have emerged from ancestors in domestic animals such as cattle or swine. The bovine coronavirus has been suggested to be a possible ancestor, from which HCoV-OC43 may have emerged in the context of a pandemic recorded historically at the end of the 19th century. New data suggest that HCoV-229E may actually be transferred from dromedary camels similar to Middle East respiratory syndrome (MERS) coronavirus. This scenario provides important ecological parallels to the present prepandemic pattern of host associations of the MERS coronavirus. CI - © 2018 Elsevier Inc. All rights reserved. FAU - Corman, Victor M AU - Corman VM AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Berlin, Germany; German Center for Infection Research (DZIF), Berlin, Germany. FAU - Muth, Doreen AU - Muth D AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Berlin, Germany; German Center for Infection Research (DZIF), Berlin, Germany. FAU - Niemeyer, Daniela AU - Niemeyer D AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Berlin, Germany. FAU - Drosten, Christian AU - Drosten C AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Berlin, Germany; German Center for Infection Research (DZIF), Berlin, Germany. Electronic address: christian.drosten@charite.de. LA - eng PT - Journal Article PT - Review DEP - 20180216 PL - United States TA - Adv Virus Res JT - Advances in virus research JID - 0370441 SB - IM MH - Animals MH - Coronavirus/*classification/*physiology MH - Coronavirus Infections/*transmission/*virology MH - *Endemic Diseases MH - Host Specificity MH - Host-Pathogen Interactions MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/classification/physiology MH - *Phylogeny MH - Zoonoses/transmission/virology PMC - PMC7112090 OTO - NOTNLM OT - Alphacoronavirus OT - Betacoronavirus OT - Chiroptera OT - Coronaviridae OT - Livestock OT - Respiratory tract infections OT - Rodentia OT - Zoonotic diseases EDAT- 2018/03/20 06:00 MHDA- 2018/12/12 06:00 PMCR- 2018/02/16 CRDT- 2018/03/20 06:00 PHST- 2018/03/20 06:00 [entrez] PHST- 2018/03/20 06:00 [pubmed] PHST- 2018/12/12 06:00 [medline] PHST- 2018/02/16 00:00 [pmc-release] AID - S0065-3527(18)30001-0 [pii] AID - 10.1016/bs.aivir.2018.01.001 [doi] PST - ppublish SO - Adv Virus Res. 2018;100:163-188. doi: 10.1016/bs.aivir.2018.01.001. Epub 2018 Feb 16. PMID- 25153347 OWN - NLM STAT- MEDLINE DCOM- 20150330 LR - 20211021 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 6 IP - 8 DP - 2014 Aug 22 TI - Canine enteric coronaviruses: emerging viral pathogens with distinct recombinant spike proteins. PG - 3363-76 LID - 10.3390/v6083363 [doi] AB - Canine enteric coronavirus (CCoV) is an alphacoronavirus infecting dogs that is closely related to enteric coronaviruses of cats and pigs. While CCoV has traditionally caused mild gastro-intestinal clinical signs, there are increasing reports of lethal CCoV infections in dogs, with evidence of both gastrointestinal and systemic viral dissemination. Consequently, CCoV is now considered to be an emerging infectious disease of dogs. In addition to the two known serotypes of CCoV, novel recombinant variants of CCoV have been found containing spike protein N-terminal domains (NTDs) that are closely related to those of feline and porcine strains. The increase in disease severity in dogs and the emergence of novel CCoVs can be attributed to the high level of recombination within the spike gene that can occur during infection by more than one CCoV type in the same host. FAU - Licitra, Beth N AU - Licitra BN AD - Department of Microbiology & Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA. bnm4@cornell.edu. FAU - Duhamel, Gerald E AU - Duhamel GE AD - Department of Biomedical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA. ged36@cornell.edu. FAU - Whittaker, Gary R AU - Whittaker GR AD - Department of Microbiology & Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA. grw7@cornell.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20140822 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Recombinant Proteins) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Communicable Diseases, Emerging/*veterinary/virology MH - Coronavirus/classification/genetics/immunology/*isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - Dog Diseases/*virology MH - Dogs MH - Recombinant Proteins/genetics MH - Recombination, Genetic MH - Serogroup MH - Spike Glycoprotein, Coronavirus/*genetics PMC - PMC4147700 EDAT- 2014/08/26 06:00 MHDA- 2015/03/31 06:00 PMCR- 2014/08/01 CRDT- 2014/08/26 06:00 PHST- 2014/03/31 00:00 [received] PHST- 2014/08/11 00:00 [revised] PHST- 2014/08/15 00:00 [accepted] PHST- 2014/08/26 06:00 [entrez] PHST- 2014/08/26 06:00 [pubmed] PHST- 2015/03/31 06:00 [medline] PHST- 2014/08/01 00:00 [pmc-release] AID - v6083363 [pii] AID - viruses-06-03363 [pii] AID - 10.3390/v6083363 [doi] PST - epublish SO - Viruses. 2014 Aug 22;6(8):3363-76. doi: 10.3390/v6083363. PMID- 30634396 OWN - NLM STAT- MEDLINE DCOM- 20190516 LR - 20221207 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 11 IP - 1 DP - 2019 Jan 9 TI - Bats and Coronaviruses. LID - 10.3390/v11010041 [doi] LID - 41 AB - Bats are speculated to be reservoirs of several emerging viruses including coronaviruses (CoVs) that cause serious disease in humans and agricultural animals. These include CoVs that cause severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), porcine epidemic diarrhea (PED) and severe acute diarrhea syndrome (SADS). Bats that are naturally infected or experimentally infected do not demonstrate clinical signs of disease. These observations have allowed researchers to speculate that bats are the likely reservoirs or ancestral hosts for several CoVs. In this review, we follow the CoV outbreaks that are speculated to have originated in bats. We review studies that have allowed researchers to identify unique adaptation in bats that may allow them to harbor CoVs without severe disease. We speculate about future studies that are critical to identify how bats can harbor multiple strains of CoVs and factors that enable these viruses to "jump" from bats to other mammals. We hope that this review will enable readers to identify gaps in knowledge that currently exist and initiate a dialogue amongst bat researchers to share resources to overcome present limitations. FAU - Banerjee, Arinjay AU - Banerjee A AD - Department of Pathology and Molecular Medicine, Michael DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4L8, Canada. banera9@mcmaster.ca. FAU - Kulcsar, Kirsten AU - Kulcsar K AD - Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. KKulcsar@som.umaryland.edu. FAU - Misra, Vikram AU - Misra V AD - Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada. vikram.misra@usask.ca. FAU - Frieman, Matthew AU - Frieman M AD - Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. MFrieman@som.umaryland.edu. FAU - Mossman, Karen AU - Mossman K AD - Department of Pathology and Molecular Medicine, Michael DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4L8, Canada. mossk@mcmaster.ca. LA - eng GR - R21 AI126300/AI/NIAID NIH HHS/United States GR - F32 AI136390/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20190109 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Animals MH - Asymptomatic Diseases MH - Chiroptera/*virology MH - Coronavirus/pathogenicity/*physiology MH - Coronavirus Infections MH - *Disease Outbreaks MH - Disease Reservoirs/*veterinary/virology MH - Evolution, Molecular MH - Genome, Viral MH - Humans MH - Phylogeny MH - Severe acute respiratory syndrome-related coronavirus/pathogenicity/physiology MH - Severe Acute Respiratory Syndrome MH - Swine PMC - PMC6356540 OTO - NOTNLM OT - bats OT - coronaviruses OT - immune response OT - in vitro OT - in vivo COIS- The authors declare no conflict of interest. EDAT- 2019/01/13 06:00 MHDA- 2019/05/17 06:00 PMCR- 2019/01/01 CRDT- 2019/01/13 06:00 PHST- 2018/12/20 00:00 [received] PHST- 2019/01/05 00:00 [revised] PHST- 2019/01/07 00:00 [accepted] PHST- 2019/01/13 06:00 [entrez] PHST- 2019/01/13 06:00 [pubmed] PHST- 2019/05/17 06:00 [medline] PHST- 2019/01/01 00:00 [pmc-release] AID - v11010041 [pii] AID - viruses-11-00041 [pii] AID - 10.3390/v11010041 [doi] PST - epublish SO - Viruses. 2019 Jan 9;11(1):41. doi: 10.3390/v11010041. PMID- 28214731 OWN - NLM STAT- MEDLINE DCOM- 20180226 LR - 20231113 IS - 1879-6265 (Electronic) IS - 1879-6257 (Print) IS - 1879-6257 (Linking) VI - 23 DP - 2017 Apr TI - Jumping species-a mechanism for coronavirus persistence and survival. PG - 1-7 LID - S1879-6257(16)30134-1 [pii] LID - 10.1016/j.coviro.2017.01.002 [doi] AB - Zoonotic transmission of novel viruses represents a significant threat to global public health and is fueled by globalization, the loss of natural habitats, and exposure to new hosts. For coronaviruses (CoVs), broad diversity exists within bat populations and uniquely positions them to seed future emergence events. In this review, we explore the host and viral dynamics that shape these CoV populations for survival, amplification, and possible emergence in novel hosts. CI - Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Menachery, Vineet D AU - Menachery VD AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States. FAU - Graham, Rachel L AU - Graham RL AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States. FAU - Baric, Ralph S AU - Baric RS AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States. Electronic address: rbaric@email.unc.edu. LA - eng GR - K99 AG049092/AG/NIA NIH HHS/United States GR - R01 AI110700/AI/NIAID NIH HHS/United States GR - U19 AI107810/AI/NIAID NIH HHS/United States GR - U19 AI109761/AI/NIAID NIH HHS/United States PT - Journal Article PT - Review DEP - 20170331 PL - Netherlands TA - Curr Opin Virol JT - Current opinion in virology JID - 101560941 SB - IM MH - Animals MH - Carrier State/*veterinary/virology MH - Coronavirus/*physiology MH - Coronavirus Infections/*veterinary/*virology MH - *Disease Reservoirs MH - Humans MH - *Microbial Viability MH - Zoonoses/*virology PMC - PMC5474123 MID - NIHMS853079 EDAT- 2017/02/20 06:00 MHDA- 2018/02/27 06:00 PMCR- 2017/03/31 CRDT- 2017/02/20 06:00 PHST- 2016/12/06 00:00 [received] PHST- 2017/01/01 00:00 [revised] PHST- 2017/01/16 00:00 [accepted] PHST- 2017/02/20 06:00 [pubmed] PHST- 2018/02/27 06:00 [medline] PHST- 2017/02/20 06:00 [entrez] PHST- 2017/03/31 00:00 [pmc-release] AID - S1879-6257(16)30134-1 [pii] AID - 10.1016/j.coviro.2017.01.002 [doi] PST - ppublish SO - Curr Opin Virol. 2017 Apr;23:1-7. doi: 10.1016/j.coviro.2017.01.002. Epub 2017 Mar 31. PMID- 27086031 OWN - NLM STAT- MEDLINE DCOM- 20171222 LR - 20200407 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 226 DP - 2016 Dec 2 TI - Porcine deltacoronavirus infection: Etiology, cell culture for virus isolation and propagation, molecular epidemiology and pathogenesis. PG - 50-59 LID - S0168-1702(16)30156-3 [pii] LID - 10.1016/j.virusres.2016.04.009 [doi] AB - Porcine deltacoronavirus (PDCoV) (family Coronaviridae, genus Deltacoronavirus) is a novel swine enteropathogenic coronavirus that causes acute diarrhea/vomiting, dehydration and mortality in seronegative neonatal piglets. PDCoV diarrhea was first reported in the US in early 2014, concurrently with co-circulation of porcine epidemic diarrhea virus (PEDV) (family Coronaviridae, genus Alphacoronavirus). The origin of PDCoV in pigs and also its sudden emergence or route of introduction into the US still remains unclear. In the US, since 2013-2014, the newly emerged PDCoV and PEDV have spread nationwide, causing a high number of pig deaths and significant economic impacts. The current US PDCoV strains are enteropathogenic and infect villous epithelial cells of the entire small and large intestines although the jejunum and ileum are the primary sites of infection. Similar to PEDV infections, PDCoV infections also cause acute, severe atrophic enteritis accompanied by transient viremia (viral RNA) that leads to severe diarrhea and/or vomiting, followed by dehydration as the potential cause of death in nursing piglets. At present, differential diagnosis of PDCoV, PEDV, and transmissible gastroenteritis virus (TGEV) is essential to control viral diarrheas in US swine. Cell culture-adapted US PDCoV (TC-PDCoV) strains have been isolated and propagated by us and in several other laboratories. TC-PDCoV strains will be useful to develop serologic assays and to evaluate if serial cell-culture passage attenuates TC-PDCoV as a potential vaccine candidate strain. A comprehensive understanding of the pathogenesis and epidemiology of epidemic PDCoV strains is currently needed to prevent and control the disease in affected regions and to develop an effective vaccine. This review focuses on the etiology, cell culture isolation and propagation, molecular epidemiology, disease mechanisms and pathogenesis of PDCoV infection. CI - Copyright © 2016 Elsevier B.V. All rights reserved. FAU - Jung, Kwonil AU - Jung K AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA. Electronic address: jung.221@osu.edu. FAU - Hu, Hui AU - Hu H AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China. FAU - Saif, Linda J AU - Saif LJ AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA. Electronic address: saif.2@osu.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Review DEP - 20160413 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 SB - IM MH - Animals MH - Cell Culture Techniques MH - Coronavirus/*classification/*genetics/isolation & purification/pathogenicity MH - Coronavirus Infections/*veterinary MH - Cross Reactions MH - Genome, Viral MH - Immunity MH - Molecular Epidemiology MH - Phylogeny MH - Swine MH - Swine Diseases/*epidemiology/*etiology/immunology/transmission MH - Virulence MH - Virus Cultivation PMC - PMC7114557 OTO - NOTNLM OT - Disease OT - Pathogenesis OT - Pigs OT - Porcine deltacoronavirus OT - Review OT - Virus EDAT- 2016/04/18 06:00 MHDA- 2017/12/23 06:00 PMCR- 2016/04/13 CRDT- 2016/04/18 06:00 PHST- 2016/03/07 00:00 [received] PHST- 2016/04/07 00:00 [revised] PHST- 2016/04/08 00:00 [accepted] PHST- 2016/04/18 06:00 [pubmed] PHST- 2017/12/23 06:00 [medline] PHST- 2016/04/18 06:00 [entrez] PHST- 2016/04/13 00:00 [pmc-release] AID - S0168-1702(16)30156-3 [pii] AID - 10.1016/j.virusres.2016.04.009 [doi] PST - ppublish SO - Virus Res. 2016 Dec 2;226:50-59. doi: 10.1016/j.virusres.2016.04.009. Epub 2016 Apr 13. PMID- 29680361 OWN - NLM STAT- MEDLINE DCOM- 20190208 LR - 20200404 IS - 1878-4380 (Electronic) IS - 0966-842X (Print) IS - 0966-842X (Linking) VI - 26 IP - 6 DP - 2018 Jun TI - Bat-Origin Coronaviruses Expand Their Host Range to Pigs. PG - 466-470 LID - S0966-842X(18)30060-X [pii] LID - 10.1016/j.tim.2018.03.001 [doi] AB - Infections with bat-origin coronaviruses have caused severe illness in humans by 'host jump'. Recently, novel bat-origin coronaviruses were found in pigs. The large number of mutations on the receptor-binding domain allowed the viruses to infect the new host, posing a potential threat to both agriculture and public health. CI - Copyright © 2018 Elsevier Ltd. All rights reserved. FAU - Wang, Liang AU - Wang L AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China. FAU - Su, Shuo AU - Su S AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, Jiangsu Engineering Laboratory of Animal Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. FAU - Bi, Yuhai AU - Bi Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China; Shenzhen Key Laboratory of Pathogen and Immunity, Guangdong Key Laboratory for Diagnosis and Treatment of Emerging Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen 518112, China. FAU - Wong, Gary AU - Wong G AD - Shenzhen Key Laboratory of Pathogen and Immunity, Guangdong Key Laboratory for Diagnosis and Treatment of Emerging Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen 518112, China. FAU - Gao, George F AU - Gao GF AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China; Shenzhen Key Laboratory of Pathogen and Immunity, Guangdong Key Laboratory for Diagnosis and Treatment of Emerging Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen 518112, China; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China. Electronic address: gaof@im.ac.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20180418 PL - England TA - Trends Microbiol JT - Trends in microbiology JID - 9310916 SB - IM MH - Alphacoronavirus/genetics MH - Animals MH - Animals, Wild/virology MH - China/epidemiology MH - Chiroptera/*virology MH - Coronavirus Infections/epidemiology/transmission/*veterinary MH - Genetic Variation MH - *Host Specificity MH - Humans MH - Phylogeny MH - Swine/*virology PMC - PMC7119057 OTO - NOTNLM OT - SeACoV OT - bat-origin OT - host jump OT - public health OT - swine enteric alphacoronaviruses EDAT- 2018/04/24 06:00 MHDA- 2019/02/09 06:00 PMCR- 2018/04/18 CRDT- 2018/04/23 06:00 PHST- 2018/01/29 00:00 [received] PHST- 2018/03/03 00:00 [revised] PHST- 2018/03/09 00:00 [accepted] PHST- 2018/04/24 06:00 [pubmed] PHST- 2019/02/09 06:00 [medline] PHST- 2018/04/23 06:00 [entrez] PHST- 2018/04/18 00:00 [pmc-release] AID - S0966-842X(18)30060-X [pii] AID - 10.1016/j.tim.2018.03.001 [doi] PST - ppublish SO - Trends Microbiol. 2018 Jun;26(6):466-470. doi: 10.1016/j.tim.2018.03.001. Epub 2018 Apr 18. PMID- 25864502 OWN - NLM STAT- MEDLINE DCOM- 20160229 LR - 20240613 IS - 1744-8395 (Electronic) IS - 1476-0584 (Print) IS - 1476-0584 (Linking) VI - 14 IP - 7 DP - 2015 Jul TI - Middle East respiratory syndrome: obstacles and prospects for vaccine development. PG - 949-62 LID - 10.1586/14760584.2015.1036033 [doi] AB - The recent emergence of Middle East respiratory syndrome (MERS) highlights the need to engineer new methods for expediting vaccine development against emerging diseases. However, several obstacles prevent pursuit of a licensable MERS vaccine. First, the lack of a suitable animal model for MERS complicates the in vivo testing of candidate vaccines. Second, due to the low number of MERS cases, pharmaceutical companies have little incentive to pursue MERS vaccine production as the costs of clinical trials are high. In addition, the timeline from bench research to approved vaccine use is 10 years or longer. Using novel methods and cost-saving strategies, genetically engineered vaccines can be produced quickly and cost-effectively. Along with progress in MERS animal model development, these obstacles can be circumvented or at least mitigated. FAU - Papaneri, Amy B AU - Papaneri AB AD - Emerging Viral Pathogens Section, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, MD, USA. FAU - Johnson, Reed F AU - Johnson RF FAU - Wada, Jiro AU - Wada J FAU - Bollinger, Laura AU - Bollinger L FAU - Jahrling, Peter B AU - Jahrling PB FAU - Kuhn, Jens H AU - Kuhn JH LA - eng GR - HHSN272200700016I/AI/NIAID NIH HHS/United States GR - ImNIH/Intramural NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Intramural PT - Research Support, U.S. Gov't, P.H.S. PT - Review DEP - 20150411 PL - England TA - Expert Rev Vaccines JT - Expert review of vaccines JID - 101155475 RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Clinical Trials as Topic MH - Coronavirus Infections/*prevention & control MH - Disease Models, Animal MH - Drug Discovery/economics/trends MH - Drug Evaluation, Preclinical/economics/methods MH - Humans MH - Viral Vaccines/*immunology/*isolation & purification PMC - PMC4832601 MID - NIHMS775260 OTO - NOTNLM OT - Coronaviridae OT - MERS-CoV OT - Middle East respiratory syndrome OT - Nidovirales OT - betacoronavirus OT - coronavirus OT - nidovirus EDAT- 2015/04/14 06:00 MHDA- 2016/03/02 06:00 PMCR- 2020/03/30 CRDT- 2015/04/14 06:00 PHST- 2015/04/14 06:00 [entrez] PHST- 2015/04/14 06:00 [pubmed] PHST- 2016/03/02 06:00 [medline] PHST- 2020/03/30 00:00 [pmc-release] AID - 1036033 [pii] AID - 10.1586/14760584.2015.1036033 [doi] PST - ppublish SO - Expert Rev Vaccines. 2015 Jul;14(7):949-62. doi: 10.1586/14760584.2015.1036033. Epub 2015 Apr 11. PMID- 26552008 OWN - NLM STAT- MEDLINE DCOM- 20160513 LR - 20221207 IS - 1546-170X (Electronic) IS - 1078-8956 (Print) IS - 1078-8956 (Linking) VI - 21 IP - 12 DP - 2015 Dec TI - A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. PG - 1508-13 LID - 10.1038/nm.3985 [doi] AB - The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome (MERS)-CoV underscores the threat of cross-species transmission events leading to outbreaks in humans. Here we examine the disease potential of a SARS-like virus, SHC014-CoV, which is currently circulating in Chinese horseshoe bat populations. Using the SARS-CoV reverse genetics system, we generated and characterized a chimeric virus expressing the spike of bat coronavirus SHC014 in a mouse-adapted SARS-CoV backbone. The results indicate that group 2b viruses encoding the SHC014 spike in a wild-type backbone can efficiently use multiple orthologs of the SARS receptor human angiotensin converting enzyme II (ACE2), replicate efficiently in primary human airway cells and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. Additionally, in vivo experiments demonstrate replication of the chimeric virus in mouse lung with notable pathogenesis. Evaluation of available SARS-based immune-therapeutic and prophylactic modalities revealed poor efficacy; both monoclonal antibody and vaccine approaches failed to neutralize and protect from infection with CoVs using the novel spike protein. On the basis of these findings, we synthetically re-derived an infectious full-length SHC014 recombinant virus and demonstrate robust viral replication both in vitro and in vivo. Our work suggests a potential risk of SARS-CoV re-emergence from viruses currently circulating in bat populations. FAU - Menachery, Vineet D AU - Menachery VD AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Yount, Boyd L Jr AU - Yount BL Jr AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Debbink, Kari AU - Debbink K AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. AD - Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Agnihothram, Sudhakar AU - Agnihothram S AD - National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas, USA. FAU - Gralinski, Lisa E AU - Gralinski LE AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Plante, Jessica A AU - Plante JA AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Graham, Rachel L AU - Graham RL AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Scobey, Trevor AU - Scobey T AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Ge, Xing-Yi AU - Ge XY AD - Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Donaldson, Eric F AU - Donaldson EF AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Randell, Scott H AU - Randell SH AD - Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. AD - Cystic Fibrosis Center, Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Lanzavecchia, Antonio AU - Lanzavecchia A AD - Institute for Research in Biomedicine, Bellinzona Institute of Microbiology, Zurich, Switzerland. FAU - Marasco, Wayne A AU - Marasco WA AD - Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA. AD - Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA. FAU - Shi, Zhengli-Li AU - Shi ZL AD - Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Baric, Ralph S AU - Baric RS AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. AD - Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. LA - eng GR - R01 AI085524/AI/NIAID NIH HHS/United States GR - AI085524/AI/NIAID NIH HHS/United States GR - T32 AI007528/AI/NIAID NIH HHS/United States GR - F32AI102561/AI/NIAID NIH HHS/United States GR - U19 AI109761/AI/NIAID NIH HHS/United States GR - DK065988/DK/NIDDK NIH HHS/United States GR - U19AI109761/AI/NIAID NIH HHS/United States GR - F32 AI102561/AI/NIAID NIH HHS/United States GR - K99 AG049092/AG/NIA NIH HHS/United States GR - U19AI107810/AI/NIAID NIH HHS/United States GR - U19 AI107810/AI/NIAID NIH HHS/United States GR - P30 DK065988/DK/NIDDK NIH HHS/United States GR - K99AG049092/AG/NIA NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20151109 PL - United States TA - Nat Med JT - Nature medicine JID - 9502015 RN - 0 (Antibodies, Monoclonal) RN - 0 (Antibodies, Viral) SB - IM EIN - Nat Med. 2016 Apr;22(4):446. doi: 10.1038/nm0416-446d. PMID: 27050591 EIN - Nat Med. 2020 Jul;26(7):1146. doi: 10.1038/s41591-020-0924-2. PMID: 32661400 MH - Animals MH - Antibodies, Monoclonal/immunology MH - Antibodies, Viral/immunology MH - Cell Line MH - Chiroptera/*virology MH - Epidemics MH - Epithelial Cells/pathology/virology MH - Female MH - Humans MH - Lung/virology MH - Mice, Inbred BALB C MH - Neutralization Tests MH - Phylogeny MH - Severe acute respiratory syndrome-related coronavirus/immunology/pathogenicity/*physiology MH - Severe Acute Respiratory Syndrome/*virology MH - Virulence MH - Virus Replication PMC - PMC4797993 MID - NIHMS766724 COIS- The authors declare no competing financial interests. EDAT- 2015/11/10 06:00 MHDA- 2016/05/14 06:00 PMCR- 2020/03/25 CRDT- 2015/11/10 06:00 PHST- 2015/06/12 00:00 [received] PHST- 2015/10/08 00:00 [accepted] PHST- 2015/11/10 06:00 [entrez] PHST- 2015/11/10 06:00 [pubmed] PHST- 2016/05/14 06:00 [medline] PHST- 2020/03/25 00:00 [pmc-release] AID - nm.3985 [pii] AID - BFnm3985 [pii] AID - 10.1038/nm.3985 [doi] PST - ppublish SO - Nat Med. 2015 Dec;21(12):1508-13. doi: 10.1038/nm.3985. Epub 2015 Nov 9. PMID- 29618817 OWN - NLM STAT- MEDLINE DCOM- 20180817 LR - 20240621 IS - 1476-4687 (Electronic) IS - 0028-0836 (Print) IS - 0028-0836 (Linking) VI - 556 IP - 7700 DP - 2018 Apr TI - Fatal swine acute diarrhoea syndrome caused by an HKU2-related coronavirus of bat origin. PG - 255-258 LID - 10.1038/s41586-018-0010-9 [doi] AB - Cross-species transmission of viruses from wildlife animal reservoirs poses a marked threat to human and animal health (1) . Bats have been recognized as one of the most important reservoirs for emerging viruses and the transmission of a coronavirus that originated in bats to humans via intermediate hosts was responsible for the high-impact emerging zoonosis, severe acute respiratory syndrome (SARS) (2-10) . Here we provide virological, epidemiological, evolutionary and experimental evidence that a novel HKU2-related bat coronavirus, swine acute diarrhoea syndrome coronavirus (SADS-CoV), is the aetiological agent that was responsible for a large-scale outbreak of fatal disease in pigs in China that has caused the death of 24,693 piglets across four farms. Notably, the outbreak began in Guangdong province in the vicinity of the origin of the SARS pandemic. Furthermore, we identified SADS-related CoVs with 96-98% sequence identity in 9.8% (58 out of 591) of anal swabs collected from bats in Guangdong province during 2013-2016, predominantly in horseshoe bats (Rhinolophus spp.) that are known reservoirs of SARS-related CoVs. We found that there were striking similarities between the SADS and SARS outbreaks in geographical, temporal, ecological and aetiological settings. This study highlights the importance of identifying coronavirus diversity and distribution in bats to mitigate future outbreaks that could threaten livestock, public health and economic growth. FAU - Zhou, Peng AU - Zhou P AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Fan, Hang AU - Fan H AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Lan, Tian AU - Lan T AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Yang, Xing-Lou AU - Yang XL AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Shi, Wei-Feng AU - Shi WF AD - Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Taishan Medical College, Taian, China. FAU - Zhang, Wei AU - Zhang W AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zhu, Yan AU - Zhu Y AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zhang, Ya-Wei AU - Zhang YW AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Xie, Qing-Mei AU - Xie QM AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Mani, Shailendra AU - Mani S AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Zheng, Xiao-Shuang AU - Zheng XS AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Li, Bei AU - Li B AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Li, Jin-Man AU - Li JM AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Guo, Hua AU - Guo H AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Pei, Guang-Qian AU - Pei GQ AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - An, Xiao-Ping AU - An XP AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Chen, Jun-Wei AU - Chen JW AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Zhou, Ling AU - Zhou L AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Mai, Kai-Jie AU - Mai KJ AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Wu, Zi-Xian AU - Wu ZX AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Li, Di AU - Li D AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Anderson, Danielle E AU - Anderson DE AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Zhang, Li-Biao AU - Zhang LB AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China. FAU - Li, Shi-Yue AU - Li SY AD - School of Public Health, Wuhan University, Wuhan, China. FAU - Mi, Zhi-Qiang AU - Mi ZQ AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - He, Tong-Tong AU - He TT AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Cong, Feng AU - Cong F AD - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. FAU - Guo, Peng-Ju AU - Guo PJ AD - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. FAU - Huang, Ren AU - Huang R AD - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. FAU - Luo, Yun AU - Luo Y AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Liu, Xiang-Ling AU - Liu XL AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Chen, Jing AU - Chen J AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Huang, Yong AU - Huang Y AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Sun, Qiang AU - Sun Q AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Zhang, Xiang-Li-Lan AU - Zhang XL AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Wang, Yuan-Yuan AU - Wang YY AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Xing, Shao-Zhen AU - Xing SZ AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Chen, Yan-Shan AU - Chen YS AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Sun, Yuan AU - Sun Y AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Li, Juan AU - Li J AD - Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Taishan Medical College, Taian, China. FAU - Daszak, Peter AU - Daszak P AD - EcoHealth Alliance, New York, NY, USA. daszak@ecohealthalliance.org. FAU - Wang, Lin-Fa AU - Wang LF AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. linfa.wang@duke-nus.edu.sg. FAU - Shi, Zheng-Li AU - Shi ZL AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. zlshi@wh.iov.cn. FAU - Tong, Yi-Gang AU - Tong YG AD - Beijing Institute of Microbiology and Epidemiology, Beijing, China. tong.yigang@gmail.com. AD - School of Life Sciences, North China University of Science and Technology, Tangshan, China. tong.yigang@gmail.com. FAU - Ma, Jing-Yun AU - Ma JY AD - College of Animal Science, South China Agricultural University, Guangzhou, China. majy2400@scau.edu.cn. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. majy2400@scau.edu.cn. LA - eng GR - R01 AI110964/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20180404 PL - England TA - Nature JT - Nature JID - 0410462 SB - IM MH - Alphacoronavirus/classification/genetics/*isolation & purification/*pathogenicity MH - Animal Diseases/*epidemiology/transmission/*virology MH - Animals MH - Biodiversity MH - China/epidemiology MH - Chiroptera/*virology MH - Coronavirus Infections/epidemiology/transmission/*veterinary MH - Diarrhea/pathology/*veterinary/virology MH - Disease Reservoirs/veterinary/virology MH - Genome, Viral/genetics MH - Humans MH - Jejunum/pathology/virology MH - Phylogeny MH - Severe Acute Respiratory Syndrome/epidemiology/veterinary/virology MH - Spatio-Temporal Analysis MH - Swine/*virology MH - Zoonoses/epidemiology/transmission/virology PMC - PMC7094983 MID - NIHMS959840 COIS- The authors declare no competing interests. EDAT- 2018/04/06 06:00 MHDA- 2018/08/18 06:00 PMCR- 2020/03/25 CRDT- 2018/04/06 06:00 PHST- 2017/07/07 00:00 [received] PHST- 2018/02/26 00:00 [accepted] PHST- 2018/04/06 06:00 [pubmed] PHST- 2018/08/18 06:00 [medline] PHST- 2018/04/06 06:00 [entrez] PHST- 2020/03/25 00:00 [pmc-release] AID - 10.1038/s41586-018-0010-9 [pii] AID - 10 [pii] AID - 10.1038/s41586-018-0010-9 [doi] PST - ppublish SO - Nature. 2018 Apr;556(7700):255-258. doi: 10.1038/s41586-018-0010-9. Epub 2018 Apr 4. PMID- 27637348 OWN - NLM STAT- MEDLINE DCOM- 20171222 LR - 20240328 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 226 DP - 2016 Dec 2 TI - From the field to the lab - An European view on the global spread of PEDV. PG - 40-49 LID - S0168-1702(16)30357-4 [pii] LID - 10.1016/j.virusres.2016.09.003 [doi] AB - Porcine Epidemic Diarrhea Virus (PEDV) is a member of the genus Alphacoronavirus, in the family Coronaviridae, of the Nidovirales order and outbreaks of porcine epidemic diarrhoea (PED) were first recorded in England in the 1970s. Intriguingly the virus has since successfully made its way around the globe, while seemingly becoming extinct in parts of Europe before its recent return from Northern America. In this review we are re-evaluating the spread of PEDV, its biology and are looking at lessons learnt from both failure and success. While a new analysis of PEDV genomes demonstrates a wider heterogeneity of PEDV than previously anticipated with at least five rather than two genotypes, biological features of the virus and its replication also point towards credible control strategies to limit the impact of this re-emerging virus. CI - Crown Copyright © 2016. Published by Elsevier B.V. All rights reserved. FAU - Choudhury, Bhudipa AU - Choudhury B AD - Virology Department, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. FAU - Dastjerdi, Akbar AU - Dastjerdi A AD - Virology Department, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. FAU - Doyle, Nicole AU - Doyle N AD - Virology Department, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. FAU - Frossard, Jean-Pierre AU - Frossard JP AD - Virology Department, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. FAU - Steinbach, Falko AU - Steinbach F AD - Virology Department, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Electronic address: falko.steinbach@apha.gsi.gov.uk. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20160913 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 SB - IM MH - Animals MH - Communicable Diseases, Emerging/veterinary MH - Coronavirus Infections/*veterinary MH - Disease Outbreaks MH - Europe/epidemiology MH - Genetic Variation MH - Genotype MH - Global Health MH - Phylogeny MH - Porcine epidemic diarrhea virus/classification/*genetics MH - Swine MH - Swine Diseases/*epidemiology/prevention & control/transmission/*virology PMC - PMC7114520 OTO - NOTNLM OT - Control OT - Diagnosis OT - Outbreak OT - PEDV OT - Sequence EDAT- 2016/09/18 06:00 MHDA- 2017/12/23 06:00 PMCR- 2016/09/13 CRDT- 2016/09/18 06:00 PHST- 2016/05/26 00:00 [received] PHST- 2016/09/05 00:00 [revised] PHST- 2016/09/08 00:00 [accepted] PHST- 2016/09/18 06:00 [pubmed] PHST- 2017/12/23 06:00 [medline] PHST- 2016/09/18 06:00 [entrez] PHST- 2016/09/13 00:00 [pmc-release] AID - S0168-1702(16)30357-4 [pii] AID - 10.1016/j.virusres.2016.09.003 [doi] PST - ppublish SO - Virus Res. 2016 Dec 2;226:40-49. doi: 10.1016/j.virusres.2016.09.003. Epub 2016 Sep 13. PMID- 28807998 OWN - NLM STAT- MEDLINE DCOM- 20180611 LR - 20240615 IS - 1091-6490 (Electronic) IS - 0027-8424 (Print) IS - 0027-8424 (Linking) VI - 114 IP - 35 DP - 2017 Aug 29 TI - Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen. PG - E7348-E7357 LID - 10.1073/pnas.1707304114 [doi] AB - Middle East respiratory syndrome coronavirus (MERS-CoV) is a lineage C betacoronavirus that since its emergence in 2012 has caused outbreaks in human populations with case-fatality rates of ∼36%. As in other coronaviruses, the spike (S) glycoprotein of MERS-CoV mediates receptor recognition and membrane fusion and is the primary target of the humoral immune response during infection. Here we use structure-based design to develop a generalizable strategy for retaining coronavirus S proteins in the antigenically optimal prefusion conformation and demonstrate that our engineered immunogen is able to elicit high neutralizing antibody titers against MERS-CoV. We also determined high-resolution structures of the trimeric MERS-CoV S ectodomain in complex with G4, a stem-directed neutralizing antibody. The structures reveal that G4 recognizes a glycosylated loop that is variable among coronaviruses and they define four conformational states of the trimer wherein each receptor-binding domain is either tightly packed at the membrane-distal apex or rotated into a receptor-accessible conformation. Our studies suggest a potential mechanism for fusion initiation through sequential receptor-binding events and provide a foundation for the structure-based design of coronavirus vaccines. FAU - Pallesen, Jesper AU - Pallesen J AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037. FAU - Wang, Nianshuang AU - Wang N AD - Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH 03755; Nianshuang.Wang@dartmouth.edu andrew@scripps.edu Jason.S.McLellan@Dartmouth.edu. FAU - Corbett, Kizzmekia S AU - Corbett KS AD - Viral Pathogenesis Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. FAU - Wrapp, Daniel AU - Wrapp D AD - Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH 03755. FAU - Kirchdoerfer, Robert N AU - Kirchdoerfer RN AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037. FAU - Turner, Hannah L AU - Turner HL AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037. FAU - Cottrell, Christopher A AU - Cottrell CA AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037. FAU - Becker, Michelle M AU - Becker MM AD - Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232. FAU - Wang, Lingshu AU - Wang L AD - Virology Core, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. FAU - Shi, Wei AU - Shi W AD - Virology Core, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. FAU - Kong, Wing-Pui AU - Kong WP AD - Virology Core, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. FAU - Andres, Erica L AU - Andres EL AD - Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232. FAU - Kettenbach, Arminja N AU - Kettenbach AN AD - Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH 03755. AD - Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756. FAU - Denison, Mark R AU - Denison MR AD - Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232. AD - Department of Pathology, Microbiology, and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232. FAU - Chappell, James D AU - Chappell JD AD - Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232. FAU - Graham, Barney S AU - Graham BS AD - Viral Pathogenesis Laboratory, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892. FAU - Ward, Andrew B AU - Ward AB AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037; Nianshuang.Wang@dartmouth.edu andrew@scripps.edu Jason.S.McLellan@Dartmouth.edu. FAU - McLellan, Jason S AU - McLellan JS AUID- ORCID: 0000-0003-3991-542X AD - Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH 03755; Nianshuang.Wang@dartmouth.edu andrew@scripps.edu Jason.S.McLellan@Dartmouth.edu. LA - eng SI - PDB/5W9H SI - PDB/5W9P SI - PDB/5VYH SI - PDB/5VZR GR - P20 GM113132/GM/NIGMS NIH HHS/United States GR - HHSN261200800001C/CA/NCI NIH HHS/United States GR - R01 AI127521/AI/NIAID NIH HHS/United States GR - P41 GM103393/GM/NIGMS NIH HHS/United States GR - HHSN261200800001E/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, N.I.H., Intramural PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20170814 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (Immunoglobulin G) RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Antibodies, Neutralizing/*immunology MH - Antibodies, Viral/immunology MH - Coronaviridae/immunology MH - Coronavirus Infections/virology MH - Crystallography, X-Ray/methods MH - Humans MH - Immunity, Humoral/immunology MH - Immunoglobulin G/metabolism MH - Mice, Inbred BALB C MH - Middle East Respiratory Syndrome Coronavirus/immunology MH - Protein Binding MH - Protein Conformation MH - Receptors, Virus/metabolism MH - Spike Glycoprotein, Coronavirus/*immunology MH - Structure-Activity Relationship MH - Vaccination MH - Viral Vaccines/immunology PMC - PMC5584442 OTO - NOTNLM OT - X-ray crystallography OT - coronavirus OT - cryo-EM OT - neutralizing antibody OT - peplomer COIS- Conflict of interest statement: J.P., N.W., K.S.C., R.N.K., H.L.T., C.A.C., B.S.G., A.B.W., and J.S.M. are inventors on US patent application no. 62/412,703, entitled “Prefusion Coronavirus Spike Proteins and Their Use.” L.W., W.S., W.-P.K., and B.S.G. are inventors on US patent application no. PCT/US2016/019395, entitled “Middle East Respiratory Syndrome Coronavirus Immunogens, Antibodies and Their Use.” EDAT- 2017/08/16 06:00 MHDA- 2018/06/12 06:00 PMCR- 2017/08/14 CRDT- 2017/08/16 06:00 PHST- 2017/08/16 06:00 [pubmed] PHST- 2018/06/12 06:00 [medline] PHST- 2017/08/16 06:00 [entrez] PHST- 2017/08/14 00:00 [pmc-release] AID - 1707304114 [pii] AID - 201707304 [pii] AID - 10.1073/pnas.1707304114 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7348-E7357. doi: 10.1073/pnas.1707304114. Epub 2017 Aug 14. PMID- 28654418 OWN - NLM STAT- MEDLINE DCOM- 20180417 LR - 20181113 IS - 1080-6059 (Electronic) IS - 1080-6040 (Print) IS - 1080-6040 (Linking) VI - 23 IP - 9 DP - 2017 Sep TI - A New Bat-HKU2-like Coronavirus in Swine, China, 2017. PG - 1607-9 LID - 10.3201/eid2309.170915 [doi] AB - We identified from suckling piglets with diarrhea in China a new bat-HKU2-like porcine coronavirus (porcine enteric alphacoronavirus). The GDS04 strain of this coronavirus shares high aa identities (>90%) with the reported bat-HKU2 strains in Coronaviridae-wide conserved domains, suggesting that the GDS04 strain belongs to the same species as HKU2. FAU - Gong, Lang AU - Gong L FAU - Li, Jie AU - Li J FAU - Zhou, Qingfeng AU - Zhou Q FAU - Xu, Zhichao AU - Xu Z FAU - Chen, Li AU - Chen L FAU - Zhang, Yun AU - Zhang Y FAU - Xue, Chunyi AU - Xue C FAU - Wen, Zhifen AU - Wen Z FAU - Cao, Yongchang AU - Cao Y LA - eng PT - Journal Article DEP - 20170917 PL - United States TA - Emerg Infect Dis JT - Emerging infectious diseases JID - 9508155 SB - IM MH - *Alphacoronavirus/classification/isolation & purification MH - Animals MH - China MH - Coronavirus Infections/*veterinary/virology MH - Diarrhea/veterinary/virology MH - Disease Outbreaks/veterinary MH - Phylogeny MH - Swine MH - Swine Diseases/*virology PMC - PMC5572857 OTO - NOTNLM OT - China OT - Coronaviridae OT - PEAV OT - bat-HKU2 OT - coronavirus OT - diarrhea OT - porcine enteric alphacoronavirus OT - swine OT - viruses OT - zoonoses EDAT- 2017/06/28 06:00 MHDA- 2018/04/18 06:00 PMCR- 2017/09/01 CRDT- 2017/06/28 06:00 PHST- 2017/06/28 06:00 [pubmed] PHST- 2018/04/18 06:00 [medline] PHST- 2017/06/28 06:00 [entrez] PHST- 2017/09/01 00:00 [pmc-release] AID - 17-0915 [pii] AID - 10.3201/eid2309.170915 [doi] PST - ppublish SO - Emerg Infect Dis. 2017 Sep;23(9):1607-9. doi: 10.3201/eid2309.170915. Epub 2017 Sep 17. PMID- 27283016 OWN - NLM STAT- MEDLINE DCOM- 20170501 LR - 20200320 IS - 1465-2099 (Electronic) IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 97 IP - 9 DP - 2016 Sep TI - Naturally occurring recombination in ferret coronaviruses revealed by complete genome characterization. PG - 2180-2186 LID - 10.1099/jgv.0.000520 [doi] AB - Ferret coronaviruses (FRCoVs) exist as an enteric and a systemic pathotype, of which the latter is highly lethal to ferrets. To our knowledge, this study provides the first full genome sequence of a FRCoV, tentatively called FRCoV-NL-2010, which was detected in 2010 in ferrets in The Netherlands. Phylogenetic analysis showed that FRCoV-NL-2010 is most closely related to mink CoV, forming a separate clade of mustelid alphacoronavirus that split off early from other alphacoronaviruses. Based on sequence homology of the complete genome, we propose that these mustelid coronaviruses may be assigned to a new species. Comparison of FRCoV-NL-2010 with the partially sequenced ferret systemic coronavirus MSU-1 and ferret enteric coronavirus MSU-2 revealed that recombination in the spike, 3c and envelope genes occurred between different FRCoVs. FAU - Lamers, Mart M AU - Lamers MM AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Smits, Saskia L AU - Smits SL AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Hundie, Gadissa B AU - Hundie GB AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Provacia, Lisette B AU - Provacia LB AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Koopmans, Marion AU - Koopmans M AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Osterhaus, Albert D M E AU - Osterhaus ADME AD - Artemis One Health, Utrecht, The Netherlands. AD - Center for Infection Medicine and Zoonoses Research, University of Veterinary Medicine, Hannover, Germany. FAU - Haagmans, Bart L AU - Haagmans BL AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Raj, V Stalin AU - Raj VS AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160607 PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Cluster Analysis MH - Coronavirus/*classification/genetics/*isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - Ferrets/*virology MH - *Genome, Viral MH - Netherlands MH - Phylogeny MH - RNA, Viral/*genetics MH - *Recombination, Genetic MH - Sequence Analysis, DNA MH - Sequence Homology PMC - PMC7079585 EDAT- 2016/06/11 06:00 MHDA- 2017/05/02 06:00 PMCR- 2016/09/01 CRDT- 2016/06/11 06:00 PHST- 2016/06/11 06:00 [entrez] PHST- 2016/06/11 06:00 [pubmed] PHST- 2017/05/02 06:00 [medline] PHST- 2016/09/01 00:00 [pmc-release] AID - 000520 [pii] AID - 10.1099/jgv.0.000520 [doi] PST - ppublish SO - J Gen Virol. 2016 Sep;97(9):2180-2186. doi: 10.1099/jgv.0.000520. Epub 2016 Jun 7. PMID- 24960574 OWN - NLM STAT- MEDLINE DCOM- 20150312 LR - 20211021 IS - 1080-6059 (Electronic) IS - 1080-6040 (Print) IS - 1080-6040 (Linking) VI - 20 IP - 7 DP - 2014 Jul TI - MERS-related betacoronavirus in Vespertilio superans bats, China. PG - 1260-2 LID - 10.3201/eid2007.140318 [doi] FAU - Yang, Li AU - Yang L FAU - Wu, Zhiqiang AU - Wu Z FAU - Ren, Xianwen AU - Ren X FAU - Yang, Fan AU - Yang F FAU - Zhang, Junpeng AU - Zhang J FAU - He, Guimei AU - He G FAU - Dong, Jie AU - Dong J FAU - Sun, Lilian AU - Sun L FAU - Zhu, Yafang AU - Zhu Y FAU - Zhang, Shuyi AU - Zhang S FAU - Jin, Qi AU - Jin Q LA - eng PT - Letter PT - Research Support, Non-U.S. Gov't PL - United States TA - Emerg Infect Dis JT - Emerging infectious diseases JID - 9508155 SB - IM MH - Animals MH - China MH - Chiroptera/*virology MH - Coronavirus/*genetics MH - Coronavirus Infections/veterinary/*virology MH - Genome, Viral/genetics MH - Middle East Respiratory Syndrome Coronavirus/*genetics MH - Phylogeny PMC - PMC4073873 OTO - NOTNLM OT - China OT - MERS-CoV OT - MERS–related betacoronavirus OT - Middle East respiratory syndrome coronavirus OT - Middle East respiratory syndrome coronavirus–related betacoronavirus OT - Vespertilio superans OT - bat OT - betacoronaviruses OT - coronavirus OT - lineage OT - lineage C betacoronavirus OT - reservoir OT - sequencing OT - viruses EDAT- 2014/06/25 06:00 MHDA- 2015/03/13 06:00 PMCR- 2014/07/01 CRDT- 2014/06/25 06:00 PHST- 2014/06/25 06:00 [entrez] PHST- 2014/06/25 06:00 [pubmed] PHST- 2015/03/13 06:00 [medline] PHST- 2014/07/01 00:00 [pmc-release] AID - 14-0318 [pii] AID - 10.3201/eid2007.140318 [doi] PST - ppublish SO - Emerg Infect Dis. 2014 Jul;20(7):1260-2. doi: 10.3201/eid2007.140318. PMID- 25811911 OWN - NLM STAT- MEDLINE DCOM- 20151204 LR - 20181113 IS - 1080-6059 (Electronic) IS - 1080-6040 (Print) IS - 1080-6040 (Linking) VI - 21 IP - 4 DP - 2015 Apr TI - Bat coronavirus in Brazil related to appalachian ridge and porcine epidemic diarrhea viruses. PG - 729-31 LID - 10.3201/eid2104.141783 [doi] FAU - Simas, Paulo Vitor Marques AU - Simas PV FAU - Barnabé, Ana Caroline de Souza AU - Barnabé AC FAU - Durães-Carvalho, Ricardo AU - Durães-Carvalho R FAU - Neto, Daniel Ferreira de Lima AU - Neto DF FAU - Caserta, Leonardo Cardia AU - Caserta LC FAU - Artacho, Luiza AU - Artacho L FAU - Jacomassa, Fábio André Facco AU - Jacomassa FA FAU - Martini, Matheus Cavalheiro AU - Martini MC FAU - Bianchi Dos Santos, Márcia Mercês Aparecida AU - Bianchi Dos Santos MM FAU - Felippe, Paulo Anselmo Nunes AU - Felippe PA FAU - Ferreira, Helena Lage AU - Ferreira HL FAU - Arns, Clarice Weis AU - Arns CW LA - eng PT - Letter PT - Research Support, Non-U.S. Gov't PL - United States TA - Emerg Infect Dis JT - Emerging infectious diseases JID - 9508155 SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronavirus Infections/*veterinary MH - Porcine epidemic diarrhea virus/classification/*genetics MH - Swine MH - Swine Diseases/*epidemiology/*virology PMC - PMC4378475 OTO - NOTNLM OT - Appalachian Ridge virus OT - Brazil OT - CoV OT - Coronavirus OT - PEDV OT - Tadarida brasiliensis OT - bats OT - porcine epidemic diarrhea virus OT - viruses EDAT- 2015/03/27 06:00 MHDA- 2015/12/15 06:00 PMCR- 2015/04/01 CRDT- 2015/03/27 06:00 PHST- 2015/03/27 06:00 [entrez] PHST- 2015/03/27 06:00 [pubmed] PHST- 2015/12/15 06:00 [medline] PHST- 2015/04/01 00:00 [pmc-release] AID - 14-1783 [pii] AID - 10.3201/eid2104.141783 [doi] PST - ppublish SO - Emerg Infect Dis. 2015 Apr;21(4):729-31. doi: 10.3201/eid2104.141783. PMID- 25113909 OWN - NLM STAT- MEDLINE DCOM- 20150110 LR - 20211021 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 464-465 DP - 2014 Sep TI - Palmitoylation of the Alphacoronavirus TGEV spike protein S is essential for incorporation into virus-like particles but dispensable for S-M interaction. PG - 397-405 LID - S0042-6822(14)00350-X [pii] LID - 10.1016/j.virol.2014.07.035 [doi] AB - The spike protein S of coronaviruses contains a highly conserved cytoplasmic cysteine-rich motif adjacent to the transmembrane region. This motif is palmitoylated in the Betacoronaviruses MHV and SARS-CoV. Here, we demonstrate by metabolic labeling with [(3)H]-palmitic acid that the S protein of transmissible gastroenteritis coronavirus (TGEV), an Alphacoronavirus, is palmitoylated as well. This is relevant for TGEV replication as virus growth was compromised by the general palmitoylation inhibitor 2-bromopalmitate. Mutation of individual cysteine clusters in the cysteine-rich motif of S revealed that all cysteines must be replaced to abolish acylation and incorporation of S into virus-like particles (VLP). Conversely, the interaction of S with the M protein, essential for VLP incorporation of S, was not impaired by lack of palmitoylation. Thus, palmitoylation of the S protein of Alphacoronaviruses is dispensable for S-M interaction, but required for the generation of progeny virions. CI - Copyright © 2014 Elsevier Inc. All rights reserved. FAU - Gelhaus, Sandra AU - Gelhaus S AD - Institute for Virology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany. FAU - Thaa, Bastian AU - Thaa B AD - Institute for Virology, Veterinary Faculty, Free University, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany. FAU - Eschke, Kathrin AU - Eschke K AD - Institute for Virology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany. FAU - Veit, Michael AU - Veit M AD - Institute for Virology, Veterinary Faculty, Free University, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany. FAU - Schwegmann-Weßels, Christel AU - Schwegmann-Weßels C AD - Institute for Virology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany. Electronic address: christel.schwegmann@tiho-hannover.de. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140809 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Protein S) RN - 0 (Viral Matrix Proteins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Cell Line MH - Lipoylation MH - Molecular Sequence Data MH - Protein Binding MH - Protein S/*chemistry/genetics/*metabolism MH - Protein Structure, Tertiary MH - Sequence Alignment MH - Swine MH - Swine Diseases/*virology MH - Transmissible gastroenteritis virus/chemistry/genetics/*metabolism MH - Viral Matrix Proteins/chemistry/genetics/*metabolism MH - Virion/chemistry/genetics/*metabolism PMC - PMC7112097 OTO - NOTNLM OT - Alphacoronavirus OT - Assembly OT - Coronavirus OT - Cysteine-rich motif OT - Palmitoylation OT - Spike protein OT - TGEV EDAT- 2014/08/13 06:00 MHDA- 2015/01/13 06:00 PMCR- 2014/08/09 CRDT- 2014/08/13 06:00 PHST- 2014/04/17 00:00 [received] PHST- 2014/05/18 00:00 [revised] PHST- 2014/07/21 00:00 [accepted] PHST- 2014/08/13 06:00 [entrez] PHST- 2014/08/13 06:00 [pubmed] PHST- 2015/01/13 06:00 [medline] PHST- 2014/08/09 00:00 [pmc-release] AID - S0042-6822(14)00350-X [pii] AID - 10.1016/j.virol.2014.07.035 [doi] PST - ppublish SO - Virology. 2014 Sep;464-465:397-405. doi: 10.1016/j.virol.2014.07.035. Epub 2014 Aug 9. PMID- 25481285 OWN - NLM STAT- MEDLINE DCOM- 20160216 LR - 20210119 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 202 DP - 2015 Apr 16 TI - Looking for a needle in a haystack: Cellular proteins that may interact with the tyrosine-based sorting signal of the TGEV S protein. PG - 3-11 LID - S0168-1702(14)00504-8 [pii] LID - 10.1016/j.virusres.2014.11.029 [doi] AB - The spike protein S of transmissible gastroenteritis virus, an Alphacoronavirus, contains a tyrosine-based sorting signal that is responsible for ERGIC retention and may be important for a correct viral assembly process. To find out whether the S protein interacts with cellular proteins via this sorting signal, a pulldown assay with GST fusion proteins was performed. Filamin A has been identified as a putative interaction candidate. Immunofluorescence assays confirmed a co-localization between the TGEV S protein and filamin A. Further experiments have to be performed to prove a significant impact of filamin A on TGEV infection. Different approaches of several researchers for the identification of cellular interaction candidates relevant for coronavirus replication are summarized. These results may help in the future to identify the role of cellular proteins during coronavirus assembly at the ER-Golgi intermediate compartment. CI - Copyright © 2014 Elsevier B.V. All rights reserved. FAU - Trincone, Anna AU - Trincone A AD - Institute for Virology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany. FAU - Schwegmann-Weßels, Christel AU - Schwegmann-Weßels C AD - Institute for Virology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany. Electronic address: christel.schwegmann@tiho-hannover.de. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20141204 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Filamins) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Filamins/*metabolism MH - *Host-Pathogen Interactions MH - Humans MH - Protein Binding MH - *Protein Interaction Mapping MH - Spike Glycoprotein, Coronavirus/*metabolism MH - Transmissible gastroenteritis virus/*physiology PMC - PMC7114463 OTO - NOTNLM OT - Assembly OT - Cellular interaction candidates OT - Coronavirus OT - Filamin A OT - Spike protein OT - TGEV EDAT- 2014/12/08 06:00 MHDA- 2016/02/18 06:00 PMCR- 2014/12/04 CRDT- 2014/12/08 06:00 PHST- 2014/08/21 00:00 [received] PHST- 2014/11/23 00:00 [revised] PHST- 2014/11/26 00:00 [accepted] PHST- 2014/12/08 06:00 [entrez] PHST- 2014/12/08 06:00 [pubmed] PHST- 2016/02/18 06:00 [medline] PHST- 2014/12/04 00:00 [pmc-release] AID - S0168-1702(14)00504-8 [pii] AID - 10.1016/j.virusres.2014.11.029 [doi] PST - ppublish SO - Virus Res. 2015 Apr 16;202:3-11. doi: 10.1016/j.virusres.2014.11.029. Epub 2014 Dec 4. PMID- 26572912 OWN - NLM STAT- MEDLINE DCOM- 20160627 LR - 20200325 IS - 1465-2099 (Electronic) IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 97 IP - 2 DP - 2016 Feb TI - Evidence for zoonotic origins of Middle East respiratory syndrome coronavirus. PG - 274-280 LID - 10.1099/jgv.0.000342 [doi] AB - Middle East respiratory syndrome (MERS) is an emerging infectious disease, caused by Middle East respiratory syndrome coronavirus (MERS-CoV) and is considered to be a zoonosis. However, the natural reservoirs of MERS-CoV remain obscure, with bats and camels as the most suspected sources. In this article, we review the evidence supporting a bat/camel origin of human MERS-CoV infection and current knowledge on the modes of camel-to-human transmission of MERS-CoV. FAU - Han, Hui-Ju AU - Han HJ AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, PR China. FAU - Yu, Hao AU - Yu H AD - School of Medicine, Fudan University, Shanghai 200433, PR China. AD - Departments of Pathology and Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. FAU - Yu, Xue-Jie AU - Yu XJ AD - Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. AD - Departments of Pathology and Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, PR China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20151113 PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 SB - IM MH - Animals MH - *Camelus MH - *Chiroptera MH - Communicable Diseases, Emerging/*epidemiology/transmission/virology MH - Coronavirus Infections/*epidemiology/transmission/virology MH - *Disease Reservoirs MH - Disease Transmission, Infectious MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*isolation & purification MH - Zoonoses/*epidemiology/transmission/virology PMC - PMC7087374 EDAT- 2015/11/18 06:00 MHDA- 2016/06/28 06:00 PMCR- 2016/02/01 CRDT- 2015/11/18 06:00 PHST- 2015/11/18 06:00 [entrez] PHST- 2015/11/18 06:00 [pubmed] PHST- 2016/06/28 06:00 [medline] PHST- 2016/02/01 00:00 [pmc-release] AID - 000342 [pii] AID - 10.1099/jgv.0.000342 [doi] PST - ppublish SO - J Gen Virol. 2016 Feb;97(2):274-280. doi: 10.1099/jgv.0.000342. Epub 2015 Nov 13. PMID- 29170370 OWN - NLM STAT- MEDLINE DCOM- 20180917 LR - 20181113 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 8 IP - 1 DP - 2017 Nov 23 TI - Receptor-binding loops in alphacoronavirus adaptation and evolution. PG - 1735 LID - 10.1038/s41467-017-01706-x [doi] LID - 1735 AB - RNA viruses are characterized by a high mutation rate, a buffer against environmental change. Nevertheless, the means by which random mutation improves viral fitness is not well characterized. Here we report the X-ray crystal structure of the receptor-binding domain (RBD) of the human coronavirus, HCoV-229E, in complex with the ectodomain of its receptor, aminopeptidase N (APN). Three extended loops are solely responsible for receptor binding and the evolution of HCoV-229E and its close relatives is accompanied by changing loop-receptor interactions. Phylogenetic analysis shows that the natural HCoV-229E receptor-binding loop variation observed defines six RBD classes whose viruses have successively replaced each other in the human population over the past 50 years. These RBD classes differ in their affinity for APN and their ability to bind an HCoV-229E neutralizing antibody. Together, our results provide a model for alphacoronavirus adaptation and evolution based on the use of extended loops for receptor binding. FAU - Wong, Alan H M AU - Wong AHM AD - Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, M5S 1A8. FAU - Tomlinson, Aidan C A AU - Tomlinson ACA AD - Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, M5S 1A8. FAU - Zhou, Dongxia AU - Zhou D AD - Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, M5S 1A8. FAU - Satkunarajah, Malathy AU - Satkunarajah M AD - Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, M5S 1A8. FAU - Chen, Kevin AU - Chen K AD - Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, M5S 1A8. FAU - Sharon, Chetna AU - Sharon C AD - Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, M5S 1A8. FAU - Desforges, Marc AU - Desforges M AD - Laboratory of Neuroimmunovirology, INRS-Institut Armand-Frappier, Institut National de la Recherche Scientifique, Université du Québec, 531 Boulevard des Prairies, Laval, Québec, Canada, H7V 1B7. FAU - Talbot, Pierre J AU - Talbot PJ AD - Laboratory of Neuroimmunovirology, INRS-Institut Armand-Frappier, Institut National de la Recherche Scientifique, Université du Québec, 531 Boulevard des Prairies, Laval, Québec, Canada, H7V 1B7. FAU - Rini, James M AU - Rini JM AD - Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, M5S 1A8. james.rini@utoronto.ca. AD - Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, M5S 1A8. james.rini@utoronto.ca. LA - eng GR - CIHR/Canada PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20171123 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.11.2 (CD13 Antigens) SB - IM MH - Adaptation, Physiological/genetics MH - Amino Acid Sequence MH - CD13 Antigens/chemistry/metabolism MH - Coronavirus 229E, Human/*genetics/pathogenicity/*physiology MH - Coronavirus Infections/virology MH - Crystallography, X-Ray MH - Evolution, Molecular MH - Genetic Variation MH - HEK293 Cells MH - Host-Pathogen Interactions/genetics MH - Humans MH - Models, Biological MH - Models, Molecular MH - Phylogeny MH - Protein Interaction Domains and Motifs MH - Receptors, Virus/chemistry/metabolism MH - Spike Glycoprotein, Coronavirus/chemistry/genetics/metabolism MH - Surface Plasmon Resonance PMC - PMC5701055 COIS- The authors declare no competing financial interests. EDAT- 2017/11/25 06:00 MHDA- 2018/09/18 06:00 PMCR- 2017/11/23 CRDT- 2017/11/25 06:00 PHST- 2017/05/29 00:00 [received] PHST- 2017/10/06 00:00 [accepted] PHST- 2017/11/25 06:00 [entrez] PHST- 2017/11/25 06:00 [pubmed] PHST- 2018/09/18 06:00 [medline] PHST- 2017/11/23 00:00 [pmc-release] AID - 10.1038/s41467-017-01706-x [pii] AID - 1706 [pii] AID - 10.1038/s41467-017-01706-x [doi] PST - epublish SO - Nat Commun. 2017 Nov 23;8(1):1735. doi: 10.1038/s41467-017-01706-x. PMID- 30791586 OWN - NLM STAT- MEDLINE DCOM- 20190917 LR - 20240715 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 11 IP - 2 DP - 2019 Feb 20 TI - Global Epidemiology of Bat Coronaviruses. LID - 10.3390/v11020174 [doi] LID - 174 AB - Bats are a unique group of mammals of the order Chiroptera. They are highly diversified and are the group of mammals with the second largest number of species. Such highly diversified cell types and receptors facilitate them to be potential hosts of a large variety of viruses. Bats are the only group of mammals capable of sustained flight, which enables them to disseminate the viruses they harbor and enhance the chance of interspecies transmission. This article aims at reviewing the various aspects of the global epidemiology of bat coronaviruses (CoVs). Before the SARS epidemic, bats were not known to be hosts for CoVs. In the last 15 years, bats have been found to be hosts of >30 CoVs with complete genomes sequenced, and many more if those without genome sequences are included. Among the four CoV genera, only alphaCoVs and betaCoVs have been found in bats. As a whole, both alphaCoVs and betaCoVs have been detected from bats in Asia, Europe, Africa, North and South America and Australasia; but alphaCoVs seem to be more widespread than betaCoVs, and their detection rate is also higher. For betaCoVs, only those from subgenera Sarbecovirus, Merbecovirus, Nobecovirus and Hibecovirus have been detected in bats. Most notably, horseshoe bats are the reservoir of SARS-CoV, and several betaCoVs from subgenus Merbecovirus are closely related to MERS-CoV. In addition to the interactions among various bat species themselves, bat⁻animal and bat⁻human interactions, such as the presence of live bats in wildlife wet markets and restaurants in Southern China, are important for interspecies transmission of CoVs and may lead to devastating global outbreaks. FAU - Wong, Antonio C P AU - Wong ACP AUID- ORCID: 0000-0002-5565-1121 AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong. antonwcp@hku.hk. FAU - Li, Xin AU - Li X AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong. lixinlyh@connect.hku.hk. FAU - Lau, Susanna K P AU - Lau SKP AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. AD - Research Centre of Infection and Immunology, The University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. AD - Carol Yu Centre for Infection, The University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. AD - Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. FAU - Woo, Patrick C Y AU - Woo PCY AUID- ORCID: 0000-0001-9401-1832 AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. AD - Research Centre of Infection and Immunology, The University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. AD - Carol Yu Centre for Infection, The University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. AD - Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20190220 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Africa/epidemiology MH - Animals MH - Asia/epidemiology MH - Australasia/epidemiology MH - China/epidemiology MH - Chiroptera/*virology MH - Coronavirus/*genetics/isolation & purification MH - Coronavirus Infections/epidemiology/*veterinary MH - Disease Reservoirs/*virology MH - Europe/epidemiology MH - *Evolution, Molecular MH - Genome, Viral MH - Global Health MH - Humans MH - North America/epidemiology MH - Phylogeny MH - Phylogeography MH - South America/epidemiology PMC - PMC6409556 OTO - NOTNLM OT - Alphacoronavirus OT - Betacoronavirus OT - bat OT - coronavirus OT - epidemiology OT - global OT - host OT - interspecies transmission COIS- The authors declare no conflict of interest. EDAT- 2019/02/23 06:00 MHDA- 2019/09/19 06:00 PMCR- 2019/02/01 CRDT- 2019/02/23 06:00 PHST- 2019/01/09 00:00 [received] PHST- 2019/02/12 00:00 [revised] PHST- 2019/02/18 00:00 [accepted] PHST- 2019/02/23 06:00 [entrez] PHST- 2019/02/23 06:00 [pubmed] PHST- 2019/09/19 06:00 [medline] PHST- 2019/02/01 00:00 [pmc-release] AID - v11020174 [pii] AID - viruses-11-00174 [pii] AID - 10.3390/v11020174 [doi] PST - epublish SO - Viruses. 2019 Feb 20;11(2):174. doi: 10.3390/v11020174. PMID- 30110630 OWN - NLM STAT- MEDLINE DCOM- 20191125 LR - 20200605 IS - 2211-1247 (Electronic) VI - 24 IP - 7 DP - 2018 Aug 14 TI - Adaptive Evolution of MERS-CoV to Species Variation in DPP4. PG - 1730-1737 LID - S2211-1247(18)31148-3 [pii] LID - 10.1016/j.celrep.2018.07.045 [doi] AB - Middle East Respiratory Syndrome Coronavirus (MERS-CoV) likely originated in bats and passed to humans through dromedary camels; however, the genetic mechanisms underlying cross-species adaptation remain poorly understood. Variation in the host receptor, dipeptidyl peptidase 4 (DPP4), can block the interaction with the MERS-CoV spike protein and form a species barrier to infection. To better understand the species adaptability of MERS-CoV, we identified a suboptimal species-derived variant of DPP4 to study viral adaption. Passaging virus on cells expressing this DPP4 variant led to accumulation of mutations in the viral spike which increased replication. Parallel passages revealed distinct paths of viral adaptation to the same DPP4 variant. Structural analysis and functional assays showed that these mutations enhanced viral entry with suboptimal DPP4 by altering the surface charge of spike. These findings demonstrate that MERS-CoV spike can utilize multiple paths to rapidly adapt to novel species variation in DPP4. CI - Published by Elsevier Inc. FAU - Letko, Michael AU - Letko M AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA. Electronic address: michael.letko@nih.gov. FAU - Miazgowicz, Kerri AU - Miazgowicz K AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA; Department of Infectious Diseases, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA. FAU - McMinn, Rebekah AU - McMinn R AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA; Department of Microbiology, Immunology, & Pathology, Colorado State University, Fort Collins, CO 80523, USA. FAU - Seifert, Stephanie N AU - Seifert SN AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA. FAU - Sola, Isabel AU - Sola I AD - Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain. FAU - Enjuanes, Luis AU - Enjuanes L AD - Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Cantoblanco, Madrid, Spain. FAU - Carmody, Aaron AU - Carmody A AD - Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA. FAU - van Doremalen, Neeltje AU - van Doremalen N AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA. FAU - Munster, Vincent AU - Munster V AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA. Electronic address: vincent.munster@nih.gov. LA - eng GR - P01 AI060699/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PL - United States TA - Cell Rep JT - Cell reports JID - 101573691 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Adaptation, Physiological MH - Amino Acid Sequence MH - Animals MH - Binding Sites MH - *Biological Coevolution MH - Chiroptera MH - Chlorocebus aethiops MH - Cricetulus MH - Dipeptidyl Peptidase 4/*chemistry/genetics/metabolism MH - Gene Expression MH - Host Specificity MH - Host-Pathogen Interactions/*genetics MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*genetics/metabolism MH - Models, Molecular MH - Mutation MH - Protein Binding MH - Protein Conformation, alpha-Helical MH - Protein Conformation, beta-Strand MH - Protein Interaction Domains and Motifs MH - Receptors, Virus/*chemistry/genetics/metabolism MH - Sequence Alignment MH - Sequence Homology, Amino Acid MH - Spike Glycoprotein, Coronavirus/*chemistry/genetics/metabolism MH - Vero Cells MH - Virus Internalization PMC - PMC7104223 OTO - NOTNLM OT - Adaptation OT - Bat OT - Coronavirus OT - DPP4 OT - Desmodus rotundus OT - Dipeptidyl peptidase IV OT - Evolution OT - MERS OT - Species barrier OT - Spike OT - Zoonosis COIS- The authors declare no competing interests. EDAT- 2018/08/16 06:00 MHDA- 2019/11/26 06:00 PMCR- 2018/08/14 CRDT- 2018/08/16 06:00 PHST- 2018/04/03 00:00 [received] PHST- 2018/06/13 00:00 [revised] PHST- 2018/07/12 00:00 [accepted] PHST- 2018/08/16 06:00 [entrez] PHST- 2018/08/16 06:00 [pubmed] PHST- 2019/11/26 06:00 [medline] PHST- 2018/08/14 00:00 [pmc-release] AID - S2211-1247(18)31148-3 [pii] AID - 10.1016/j.celrep.2018.07.045 [doi] PST - ppublish SO - Cell Rep. 2018 Aug 14;24(7):1730-1737. doi: 10.1016/j.celrep.2018.07.045. PMID- 29134417 OWN - NLM STAT- MEDLINE DCOM- 20181211 LR - 20221207 IS - 1869-1889 (Electronic) IS - 1674-7305 (Print) IS - 1674-7305 (Linking) VI - 60 IP - 12 DP - 2017 Dec TI - Cross-neutralization of SARS coronavirus-specific antibodies against bat SARS-like coronaviruses. PG - 1399-1402 LID - 10.1007/s11427-017-9189-3 [doi] FAU - Zeng, Lei-Ping AU - Zeng LP AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Ge, Xing-Yi AU - Ge XY AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Peng, Cheng AU - Peng C AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Tai, Wanbo AU - Tai W AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY, 10021, USA. FAU - Jiang, Shibo AU - Jiang S AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY, 10021, USA. AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences, Fudan University, Shanghai, 200433, China. FAU - Du, Lanying AU - Du L AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY, 10021, USA. ldu@nybc.org. FAU - Shi, Zheng-Li AU - Shi ZL AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn. LA - eng GR - R01 AI098775/AI/NIAID NIH HHS/United States GR - R01 AI110964/AI/NIAID NIH HHS/United States PT - Letter DEP - 20171110 PL - China TA - Sci China Life Sci JT - Science China. Life sciences JID - 101529880 RN - 0 (Antibodies, Monoclonal) RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (Antigens, Viral) RN - 0 (Epitopes) RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Antibodies, Monoclonal/immunology MH - Antibodies, Neutralizing/*immunology MH - Antibodies, Viral/*immunology MH - Antigens, Viral MH - Binding Sites/genetics/immunology MH - Chiroptera/*virology MH - Chlorocebus aethiops MH - Cross Reactions MH - Epitopes MH - Mice MH - Neutralization Tests MH - Receptors, Virus MH - Severe acute respiratory syndrome-related coronavirus/*classification/genetics/*immunology MH - Spike Glycoprotein, Coronavirus/classification/genetics/*immunology MH - Vero Cells PMC - PMC7089274 EDAT- 2017/11/15 06:00 MHDA- 2018/12/12 06:00 PMCR- 2020/03/23 CRDT- 2017/11/15 06:00 PHST- 2017/07/09 00:00 [received] PHST- 2017/09/16 00:00 [accepted] PHST- 2017/11/15 06:00 [pubmed] PHST- 2018/12/12 06:00 [medline] PHST- 2017/11/15 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s11427-017-9189-3 [pii] AID - 9189 [pii] AID - 10.1007/s11427-017-9189-3 [doi] PST - ppublish SO - Sci China Life Sci. 2017 Dec;60(12):1399-1402. doi: 10.1007/s11427-017-9189-3. Epub 2017 Nov 10. PMID- 28500431 OWN - NLM STAT- MEDLINE DCOM- 20180522 LR - 20200325 IS - 2095-0225 (Electronic) IS - 2095-0217 (Print) IS - 2095-0217 (Linking) VI - 11 IP - 3 DP - 2017 Sep TI - Molecular aspects of MERS-CoV. PG - 365-377 LID - 10.1007/s11684-017-0521-z [doi] AB - Middle East respiratory syndrome coronavirus (MERS-CoV) is a betacoronavirus which can cause acute respiratory distress in humans and is associated with a relatively high mortality rate. Since it was first identified in a patient who died in a Jeddah hospital in 2012, the World Health Organization has been notified of 1735 laboratory-confirmed cases from 27 countries, including 628 deaths. Most cases have occurred in Saudi Arabia. MERS-CoVancestors may be found in OldWorld bats of the Vespertilionidae family. After a proposed bat to camel switching event, transmission of MERS-CoV to humans is likely to have been the result of multiple zoonotic transfers from dromedary camels. Human-to-human transmission appears to require close contact with infected persons, with outbreaks mainly occurring in hospital environments. Outbreaks have been associated with inadequate infection prevention and control implementation, resulting in recommendations on basic and more advanced infection prevention and control measures by the World Health Organization, and issuing of government guidelines based on these recommendations in affected countries including Saudi Arabia. Evolutionary changes in the virus, particularly in the viral spike protein which mediates virus-host cell contact may potentially increase transmission of this virus. Efforts are on-going to identify specific evidence-based therapies or vaccines. The broad-spectrum antiviral nitazoxanide has been shown to have in vitro activity against MERS-CoV. Synthetic peptides and candidate vaccines based on regions of the spike protein have shown promise in rodent and non-human primate models. GLS-5300, a prophylactic DNA-plasmid vaccine encoding S protein, is the first MERS-CoV vaccine to be tested in humans, while monoclonal antibody, m336 has given promising results in animal models and has potential for use in outbreak situations. FAU - Rabaan, Ali A AU - Rabaan AA AD - Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran, 31311, Saudi Arabia. ali.rabaan@jhah.com. FAU - Bazzi, Ali M AU - Bazzi AM AD - Microbiology Laboratory, Johns Hopkins Aramco Healthcare, Dhahran, 31311, Saudi Arabia. FAU - Al-Ahmed, Shamsah H AU - Al-Ahmed SH AD - Specialty Paediatric Medicine, Qatif Central Hospital, Qatif, 32654, Saudi Arabia. FAU - Al-Tawfiq, Jaffar A AU - Al-Tawfiq JA AD - Specialty Internal Medicine, Johns Hopkins Aramco Healthcare, Dhahran, 31311, Saudi Arabia. AD - University School of Medicine, Indianapolis, IN, 46202, USA. LA - eng PT - Journal Article PT - Review DEP - 20170513 PL - China TA - Front Med JT - Frontiers of medicine JID - 101549428 RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Vaccines, DNA) SB - IM MH - Animals MH - Camelus/virology MH - Chiroptera/virology MH - Coronavirus Infections/epidemiology/prevention & control/*therapy/*transmission MH - Disease Outbreaks MH - Host-Pathogen Interactions MH - Humans MH - *Middle East Respiratory Syndrome Coronavirus MH - Mutation MH - Saudi Arabia MH - Spike Glycoprotein, Coronavirus/immunology MH - Vaccines, DNA/immunology PMC - PMC7089120 OTO - NOTNLM OT - MERS-CoV OT - Saudi Arabia OT - evolution OT - spike protein OT - transmission OT - vaccine EDAT- 2017/05/14 06:00 MHDA- 2018/05/23 06:00 PMCR- 2020/03/23 CRDT- 2017/05/14 06:00 PHST- 2016/08/16 00:00 [received] PHST- 2017/01/23 00:00 [accepted] PHST- 2017/05/14 06:00 [pubmed] PHST- 2018/05/23 06:00 [medline] PHST- 2017/05/14 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s11684-017-0521-z [pii] AID - 521 [pii] AID - 10.1007/s11684-017-0521-z [doi] PST - ppublish SO - Front Med. 2017 Sep;11(3):365-377. doi: 10.1007/s11684-017-0521-z. Epub 2017 May 13. PMID- 30832341 OWN - NLM STAT- MEDLINE DCOM- 20190918 LR - 20200309 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 11 IP - 3 DP - 2019 Mar 2 TI - Bat Coronaviruses in China. LID - 10.3390/v11030210 [doi] LID - 210 AB - During the past two decades, three zoonotic coronaviruses have been identified as the cause of large-scale disease outbreaks⁻Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (MERS), and Swine Acute Diarrhea Syndrome (SADS). SARS and MERS emerged in 2003 and 2012, respectively, and caused a worldwide pandemic that claimed thousands of human lives, while SADS struck the swine industry in 2017. They have common characteristics, such as they are all highly pathogenic to humans or livestock, their agents originated from bats, and two of them originated in China. Thus, it is highly likely that future SARS- or MERS-like coronavirus outbreaks will originate from bats, and there is an increased probability that this will occur in China. Therefore, the investigation of bat coronaviruses becomes an urgent issue for the detection of early warning signs, which in turn minimizes the impact of such future outbreaks in China. The purpose of the review is to summarize the current knowledge on viral diversity, reservoir hosts, and the geographical distributions of bat coronaviruses in China, and eventually we aim to predict virus hotspots and their cross-species transmission potential. FAU - Fan, Yi AU - Fan Y AUID- ORCID: 0000-0002-4117-8433 AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. yifanfs0224@163.com. AD - University of Chinese Academy of Sciences, Beijing 100049, China. yifanfs0224@163.com. FAU - Zhao, Kai AU - Zhao K AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. chiukal@163.com. AD - University of Chinese Academy of Sciences, Beijing 100049, China. chiukal@163.com. FAU - Shi, Zheng-Li AU - Shi ZL AUID- ORCID: 0000-0001-8089-163X AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. zlshi@wh.iov.cn. AD - University of Chinese Academy of Sciences, Beijing 100049, China. zlshi@wh.iov.cn. FAU - Zhou, Peng AU - Zhou P AUID- ORCID: 0000-0001-9863-4201 AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. peng.zhou@wh.iov.cn. AD - University of Chinese Academy of Sciences, Beijing 100049, China. peng.zhou@wh.iov.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20190302 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Animals MH - China/epidemiology MH - Chiroptera/*virology MH - Coronavirus/*genetics/isolation & purification MH - Coronavirus Infections/epidemiology/veterinary MH - Disease Outbreaks/prevention & control MH - Disease Reservoirs/*virology MH - *Evolution, Molecular MH - Genetic Variation MH - Genome, Viral MH - Humans MH - Phylogeny MH - Zoonoses/epidemiology/transmission/*virology PMC - PMC6466186 OTO - NOTNLM OT - bat OT - coronavirus OT - cross-species OT - epidemiology OT - zoonosis COIS- The authors declare no conflict of interest. EDAT- 2019/03/06 06:00 MHDA- 2019/09/19 06:00 PMCR- 2019/03/01 CRDT- 2019/03/06 06:00 PHST- 2019/01/29 00:00 [received] PHST- 2019/02/26 00:00 [revised] PHST- 2019/02/26 00:00 [accepted] PHST- 2019/03/06 06:00 [entrez] PHST- 2019/03/06 06:00 [pubmed] PHST- 2019/09/19 06:00 [medline] PHST- 2019/03/01 00:00 [pmc-release] AID - v11030210 [pii] AID - viruses-11-00210 [pii] AID - 10.3390/v11030210 [doi] PST - epublish SO - Viruses. 2019 Mar 2;11(3):210. doi: 10.3390/v11030210. PMID- 28820366 OWN - NLM STAT- MEDLINE DCOM- 20180426 LR - 20181113 IS - 1080-6059 (Electronic) IS - 1080-6040 (Print) IS - 1080-6040 (Linking) VI - 23 IP - 9 DP - 2017 Sep TI - Determination of Ferret Enteric Coronavirus Genome in Laboratory Ferrets. PG - 1568-1570 LID - 10.3201/eid2309.160215 [doi] AB - Ferret enteric coronavirus (FRECV) RNA was detected in laboratory ferrets. Analysis of the complete genome sequence of 2 strains, FRCoV4370 and FRCoV063, revealed that FRECV shared 49.9%-68.9% nucleotide sequence identity with known coronaviruses. These results suggest that FRECV might be classified as a new species in the genus Alphacoronavirus. FAU - Li, Tian-Cheng AU - Li TC FAU - Yoshizaki, Sayaka AU - Yoshizaki S FAU - Kataoka, Michiyo AU - Kataoka M FAU - Doan, Yen Hai AU - Doan YH FAU - Ami, Yasushi AU - Ami Y FAU - Suzaki, Yuriko AU - Suzaki Y FAU - Nakamura, Tomofumi AU - Nakamura T FAU - Takeda, Naokazu AU - Takeda N FAU - Wakita, Takaji AU - Wakita T LA - eng PT - Journal Article PL - United States TA - Emerg Infect Dis JT - Emerging infectious diseases JID - 9508155 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Animals, Laboratory MH - Asymptomatic Diseases MH - Coronavirus/classification/*genetics/isolation & purification MH - Coronavirus Infections/diagnosis/*veterinary/virology MH - Feces/virology MH - Ferrets/*virology MH - *Genome, Viral MH - Japan MH - Open Reading Frames MH - Phylogeny MH - RNA, Viral/*genetics MH - Sequence Analysis, DNA PMC - PMC5572892 OTO - NOTNLM OT - Ferret enteric coronavirus OT - coronavirus OT - enteric infections OT - ferret OT - ferret systemic coronavirus OT - genome OT - next-generation sequencing OT - viruses EDAT- 2017/08/19 06:00 MHDA- 2018/04/27 06:00 PMCR- 2017/09/01 CRDT- 2017/08/19 06:00 PHST- 2017/08/19 06:00 [entrez] PHST- 2017/08/19 06:00 [pubmed] PHST- 2018/04/27 06:00 [medline] PHST- 2017/09/01 00:00 [pmc-release] AID - 16-0215 [pii] AID - 10.3201/eid2309.160215 [doi] PST - ppublish SO - Emerg Infect Dis. 2017 Sep;23(9):1568-1570. doi: 10.3201/eid2309.160215. PMID- 29500691 OWN - NLM STAT- MEDLINE DCOM- 20181001 LR - 20240402 IS - 1995-820X (Electronic) IS - 1674-0769 (Print) IS - 1995-820X (Linking) VI - 33 IP - 1 DP - 2018 Feb TI - Serological Evidence of Bat SARS-Related Coronavirus Infection in Humans, China. PG - 104-107 LID - 10.1007/s12250-018-0012-7 [doi] FAU - Wang, Ning AU - Wang N AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Li, Shi-Yue AU - Li SY AD - School of Health Sciences, Wuhan University, Wuhan, 430071, China. FAU - Yang, Xing-Lou AU - Yang XL AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Huang, Hui-Min AU - Huang HM AD - School of Health Sciences, Wuhan University, Wuhan, 430071, China. FAU - Zhang, Yu-Ji AU - Zhang YJ AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Guo, Hua AU - Guo H AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Luo, Chu-Ming AU - Luo CM AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Miller, Maureen AU - Miller M AD - EcoHealth Alliance, New York, NY, 10001, USA. FAU - Zhu, Guangjian AU - Zhu G AD - EcoHealth Alliance, New York, NY, 10001, USA. FAU - Chmura, Aleksei A AU - Chmura AA AD - EcoHealth Alliance, New York, NY, 10001, USA. FAU - Hagan, Emily AU - Hagan E AD - EcoHealth Alliance, New York, NY, 10001, USA. FAU - Zhou, Ji-Hua AU - Zhou JH AD - Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. FAU - Zhang, Yun-Zhi AU - Zhang YZ AD - Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. AD - School of Public Health, Dali University, Dali, 671000, China. FAU - Wang, Lin-Fa AU - Wang LF AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, 169857, Singapore. FAU - Daszak, Peter AU - Daszak P AD - EcoHealth Alliance, New York, NY, 10001, USA. FAU - Shi, Zheng-Li AU - Shi ZL AUID- ORCID: 0000-0001-8089-163X AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn. LA - eng GR - R01 AI110964/AI/NIAID NIH HHS/United States PT - Letter DEP - 20180302 PL - Netherlands TA - Virol Sin JT - Virologica Sinica JID - 101514185 RN - 0 (Antibodies, Viral) SB - IM MH - Adolescent MH - Adult MH - Aged MH - Aged, 80 and over MH - Animals MH - Antibodies, Viral/*blood MH - Child MH - China/epidemiology MH - Chiroptera/virology MH - Enzyme-Linked Immunosorbent Assay MH - Female MH - Humans MH - Male MH - Middle Aged MH - Severe acute respiratory syndrome-related coronavirus/*immunology MH - *Serologic Tests MH - Severe Acute Respiratory Syndrome/*diagnosis/epidemiology MH - Young Adult MH - Zoonoses/*diagnosis/epidemiology/virology PMC - PMC6178078 COIS- CONFLICT OF INTEREST: The authors declare that they have no conflict of interest. ANIMAL AND HUMAN RIGHTS: This study was approved by the Wuhan Institute of Virology Institutional Review Board (China) and by Hummingbird IRB (USA). EDAT- 2018/03/04 06:00 MHDA- 2018/10/03 06:00 PMCR- 2019/03/02 CRDT- 2018/03/04 06:00 PHST- 2017/11/21 00:00 [received] PHST- 2018/01/08 00:00 [accepted] PHST- 2018/03/04 06:00 [pubmed] PHST- 2018/10/03 06:00 [medline] PHST- 2018/03/04 06:00 [entrez] PHST- 2019/03/02 00:00 [pmc-release] AID - 10.1007/s12250-018-0012-7 [pii] AID - 12 [pii] AID - 10.1007/s12250-018-0012-7 [doi] PST - ppublish SO - Virol Sin. 2018 Feb;33(1):104-107. doi: 10.1007/s12250-018-0012-7. Epub 2018 Mar 2. PMID- 27102167 OWN - NLM STAT- MEDLINE DCOM- 20161107 LR - 20181113 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 8 IP - 3 DP - 2016 Mar 18 TI - Discovery of Novel Alphacoronaviruses in European Rodents and Shrews. PG - 84 LID - 10.3390/v8030084 [doi] LID - 84 AB - Eight hundred and thirteen European rodents and shrews encompassing seven different species were screened for alphacoronaviruses using PCR detection. Novel alphacoronaviruses were detected in the species Rattus norvegicus, Microtus agrestis, Sorex araneus and Myodes glareolus. These, together with the recently described Lucheng virus found in China, form a distinct rodent/shrew-specific clade within the coronavirus phylogeny. Across a highly conserved region of the viral polymerase gene, the new members of this clade were up to 22% dissimilar at the nucleotide level to the previously described Lucheng virus. As such they might represent distinct species of alphacoronaviruses. These data greatly extend our knowledge of wildlife reservoirs of alphacoronaviruses. FAU - Tsoleridis, Theocharis AU - Tsoleridis T AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. stxtt6@exmail.nottingham.ac.uk. FAU - Onianwa, Okechukwu AU - Onianwa O AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. nixoo@exmail.nottingham.ac.uk. FAU - Horncastle, Emma AU - Horncastle E AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. stxerh@nottingham.ac.uk. FAU - Dayman, Emma AU - Dayman E AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. stxed3@nottingham.ac.uk. FAU - Zhu, Miaoran AU - Zhu M AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. msxmz@nottingham.ac.uk. FAU - Danjittrong, Taechasit AU - Danjittrong T AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. taechasit@live.com. FAU - Wachtl, Marta AU - Wachtl M AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. mzymw3@nottingham.ac.uk. FAU - Behnke, Jerzy M AU - Behnke JM AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. J.Behnke@nottingham.ac.uk. FAU - Chapman, Sarah AU - Chapman S AD - Twycross Zoo, Burton Road, Atherstone, Warwickshire CV9 3PX, UK. sarah.chapman@twycrosszoo.org. FAU - Strong, Victoria AU - Strong V AD - Twycross Zoo, Burton Road, Atherstone, Warwickshire CV9 3PX, UK. ntxvjs@exmail.nottingham.ac.uk. FAU - Dobbs, Phillipa AU - Dobbs P AD - Twycross Zoo, Burton Road, Atherstone, Warwickshire CV9 3PX, UK. phillipa.dobbs@twycrosszoo.org. FAU - Ball, Jonathan K AU - Ball JK AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. jonathan.ball@nottingham.ac.uk. FAU - Tarlinton, Rachael E AU - Tarlinton RE AD - School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK. rachael.tarlinton@nottingham.ac.uk. FAU - McClure, C Patrick AU - McClure CP AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. patrick.mcclure@nottingham.ac.uk. LA - eng GR - Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160318 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Viral Proteins) SB - IM MH - Animals MH - Coronavirus/*classification/*isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - Europe MH - Genotype MH - Mass Screening MH - Phylogeny MH - Polymerase Chain Reaction MH - Rodentia/*virology MH - Sequence Analysis, DNA MH - Shrews/*virology MH - Viral Proteins/genetics PMC - PMC4810274 OTO - NOTNLM OT - alphacoronavirus OT - coronaviruses OT - rodents OT - shrews EDAT- 2016/04/23 06:00 MHDA- 2016/11/08 06:00 PMCR- 2016/03/01 CRDT- 2016/04/23 06:00 PHST- 2015/12/04 00:00 [received] PHST- 2016/02/29 00:00 [revised] PHST- 2016/03/11 00:00 [accepted] PHST- 2016/04/23 06:00 [entrez] PHST- 2016/04/23 06:00 [pubmed] PHST- 2016/11/08 06:00 [medline] PHST- 2016/03/01 00:00 [pmc-release] AID - v8030084 [pii] AID - viruses-08-00084 [pii] AID - 10.3390/v8030084 [doi] PST - epublish SO - Viruses. 2016 Mar 18;8(3):84. doi: 10.3390/v8030084. PMID- 30849413 OWN - NLM STAT- MEDLINE DCOM- 20190819 LR - 20240328 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 265 DP - 2019 May TI - PEDV nsp16 negatively regulates innate immunity to promote viral proliferation. PG - 57-66 LID - S0168-1702(18)30625-7 [pii] LID - 10.1016/j.virusres.2019.03.005 [doi] AB - Type-I IFNs (IFN-I) provide a key mediator of innate antiviral response during virus proliferation. Porcine epidemic diarrhea virus (PEDV), which causes diarrhea in swine of all ages, is a worldwide-distributed alphacoronavirus with economic importance. Here, we screened PEDV RNA modification enzymes involved in regulating antiviral response. Whereas the PEDV nsp13 barely regulates type I IFN, inflammatory cytokines (IL-6, TNF-a) and MHCII, nsp16 and nsp14 (to a lesser extent) down-regulate these antiviral effectors. Importantly, we found nsp16 KDKE tetrad appears to play a key role in interferon inhibition by mutating the D129 catalytic residue. Mechanistically, nsp16 down-regulates the activities of RIG-I and MDA5 mediated IFN-β and ISRE. In turn, the mRNA levels of IFIT family members (IFIT1, IFIT2, IFIT3) was inhibited in cells overexpressing nsp16. In addition, nsp10 enhanced the inhibitory effect of nsp16 on IFN-β. Altogether these results indicate PEDV nsp16 negatively regulates innate immunity to promote viral proliferation. Findings from this study provides novel perspective to advance the understanding in the pathogenesis of PEDV. CI - Copyright © 2019. Published by Elsevier B.V. FAU - Shi, Peidian AU - Shi P AD - School of Life Sciences, Tianjin University, Tianjin, 300072, China. FAU - Su, Yanxin AU - Su Y AD - School of Life Sciences, Tianjin University, Tianjin, 300072, China. FAU - Li, Ruiqiao AU - Li R AD - School of Life Sciences, Tianjin University, Tianjin, 300072, China. FAU - Liang, Zhixuan AU - Liang Z AD - Tianjin Center of Animal Disease Preventive and Control, Tianjin, China. FAU - Dong, Shuren AU - Dong S AD - Ninghe breeding pig farm of Tianjin, China. FAU - Huang, Jinhai AU - Huang J AD - School of Life Sciences, Tianjin University, Tianjin, 300072, China. Electronic address: jinhaih@tju.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190305 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Viral Nonstructural Proteins) RN - 77238-31-4 (Interferon-beta) RN - EC 3.6.4.13 (DEAD Box Protein 58) RN - EC 3.6.4.13 (Interferon-Induced Helicase, IFIH1) MH - Animals MH - Cell Line MH - DEAD Box Protein 58/genetics MH - Down-Regulation MH - *Host-Pathogen Interactions MH - *Immunity, Innate MH - Interferon-Induced Helicase, IFIH1/genetics MH - Interferon-beta/*genetics MH - Porcine epidemic diarrhea virus/*genetics/physiology MH - Signal Transduction MH - Swine MH - Viral Nonstructural Proteins/*genetics MH - Virus Replication PMC - PMC7114654 OTO - NOTNLM OT - 2'O-MTase OT - IFIT OT - IFN-β OT - PEDV OT - nsp16 EDAT- 2019/03/09 06:00 MHDA- 2019/08/20 06:00 PMCR- 2019/03/05 CRDT- 2019/03/09 06:00 PHST- 2018/10/16 00:00 [received] PHST- 2019/02/22 00:00 [revised] PHST- 2019/03/04 00:00 [accepted] PHST- 2019/03/09 06:00 [pubmed] PHST- 2019/08/20 06:00 [medline] PHST- 2019/03/09 06:00 [entrez] PHST- 2019/03/05 00:00 [pmc-release] AID - S0168-1702(18)30625-7 [pii] AID - 10.1016/j.virusres.2019.03.005 [doi] PST - ppublish SO - Virus Res. 2019 May;265:57-66. doi: 10.1016/j.virusres.2019.03.005. Epub 2019 Mar 5. PMID- 26049252 OWN - NLM STAT- MEDLINE DCOM- 20150922 LR - 20220330 IS - 1474-547X (Electronic) IS - 0140-6736 (Print) IS - 0140-6736 (Linking) VI - 386 IP - 9997 DP - 2015 Sep 5 TI - Middle East respiratory syndrome. PG - 995-1007 LID - S0140-6736(15)60454-8 [pii] LID - 10.1016/S0140-6736(15)60454-8 [doi] AB - Middle East respiratory syndrome (MERS) is a highly lethal respiratory disease caused by a novel single-stranded, positive-sense RNA betacoronavirus (MERS-CoV). Dromedary camels, hosts for MERS-CoV, are implicated in direct or indirect transmission to human beings, although the exact mode of transmission is unknown. The virus was first isolated from a patient who died from a severe respiratory illness in June, 2012, in Jeddah, Saudi Arabia. As of May 31, 2015, 1180 laboratory-confirmed cases (483 deaths; 40% mortality) have been reported to WHO. Both community-acquired and hospital-acquired cases have been reported with little human-to-human transmission reported in the community. Although most cases of MERS have occurred in Saudi Arabia and the United Arab Emirates, cases have been reported in Europe, the USA, and Asia in people who travelled from the Middle East or their contacts. Clinical features of MERS range from asymptomatic or mild disease to acute respiratory distress syndrome and multiorgan failure resulting in death, especially in individuals with underlying comorbidities. No specific drug treatment exists for MERS and infection prevention and control measures are crucial to prevent spread in health-care facilities. MERS-CoV continues to be an endemic, low-level public health threat. However, the virus could mutate to have increased interhuman transmissibility, increasing its pandemic potential. CI - Copyright © 2015 Elsevier Ltd. All rights reserved. FAU - Zumla, Alimuddin AU - Zumla A AD - Division of Infection and Immunity, University College London, London, UK; NIHR Biomedical Research Centre, UCL Hospitals NHS Foundation Trust, London, UK. FAU - Hui, David S AU - Hui DS AD - Division of Respiratory Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, New Territories, Hong Kong Special Administrative Region, China; Stanley Ho Center for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, New Territories, Hong Kong Special Administrative Region, China. FAU - Perlman, Stanley AU - Perlman S AD - Department of Microbiology, University of Iowa, Iowa City, IA, USA; Department of Pediatrics, University of Iowa, Iowa City, IA, USA. Electronic address: stanley-perlman@uiowa.edu. LA - eng GR - P01 AI060699/AI/NIAID NIH HHS/United States GR - R01 AI091322/AI/NIAID NIH HHS/United States GR - AI060699/AI/NIAID NIH HHS/United States GR - AI091322/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Review DEP - 20150603 PL - England TA - Lancet JT - Lancet (London, England) JID - 2985213R SB - IM MH - Adolescent MH - Adult MH - Aged MH - Aged, 80 and over MH - Animals MH - Camelus/virology MH - Child MH - Child, Preschool MH - *Coronavirus Infections/diagnosis/epidemiology/therapy/transmission MH - Epidemics MH - Female MH - Humans MH - Infant MH - Male MH - Middle Aged MH - Middle East MH - Middle East Respiratory Syndrome Coronavirus/*pathogenicity MH - Young Adult PMC - PMC4721578 MID - NIHMS750472 EDAT- 2015/06/08 06:00 MHDA- 2015/09/24 06:00 PMCR- 2015/06/03 CRDT- 2015/06/08 06:00 PHST- 2015/06/08 06:00 [entrez] PHST- 2015/06/08 06:00 [pubmed] PHST- 2015/09/24 06:00 [medline] PHST- 2015/06/03 00:00 [pmc-release] AID - S0140-6736(15)60454-8 [pii] AID - 10.1016/S0140-6736(15)60454-8 [doi] PST - ppublish SO - Lancet. 2015 Sep 5;386(9997):995-1007. doi: 10.1016/S0140-6736(15)60454-8. Epub 2015 Jun 3. PMID- 29429482 OWN - NLM STAT- MEDLINE DCOM- 20180814 LR - 20240328 IS - 1532-2971 (Electronic) IS - 1090-0233 (Print) IS - 1090-0233 (Linking) VI - 231 DP - 2018 Jan TI - Enteric coronavirus infection in adult horses. PG - 13-18 LID - S1090-0233(17)30222-8 [pii] LID - 10.1016/j.tvjl.2017.11.004 [doi] AB - A new enteric virus of adult horses, equine coronavirus (ECoV), has recently been recognized. It is associated with fever, lethargy, anorexia, and less frequently, colic and diarrhea. This enteric virus is transmitted via the feco-oral route and horses become infected by ingesting fecally contaminated feed and water. Various outbreaks have been reported since 2010 from Japan, Europe and the USA. While the clinical signs are fairly non-specific, lymphopenia and neutropenia are often seen. Specific diagnosis is made by the detection of ECoV in feces by either quantitative real-time PCR, electron microscopy or antigen-capture ELISA. Supportive treatment is usually required, as most infections are self-limiting. However, rare complications, such as endotoxemia, septicemia and hyperammonemia-associated encephalopathy, have been reported, and have been related to the loss of barrier function at the intestinal mucosa. This review article will focus on the latest information pertaining to the virus, epidemiology, clinical signs, diagnosis, pathology, treatment and prevention of ECoV infection in adult horses. CI - Copyright © 2017 Elsevier Ltd. All rights reserved. FAU - Pusterla, N AU - Pusterla N AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. Electronic address: npusterla@ucdavis.edu. FAU - Vin, R AU - Vin R AD - Myhre Equine Clinic, Rochester, NH 03867, USA. FAU - Leutenegger, C M AU - Leutenegger CM AD - IDEXX Laboratories, West Sacramento, CA 95605, USA. FAU - Mittel, L D AU - Mittel LD AD - College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. FAU - Divers, T J AU - Divers TJ AD - College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. LA - eng PT - Journal Article PT - Review DEP - 20171120 PL - England TA - Vet J JT - Veterinary journal (London, England : 1997) JID - 9706281 SB - IM MH - Animals MH - Betacoronavirus 1/*physiology MH - Coronavirus Infections/diagnosis/epidemiology/prevention & control/*veterinary MH - *Horse Diseases/diagnosis/epidemiology/pathology/prevention & control MH - Horses PMC - PMC7110460 OTO - NOTNLM OT - Clinical disease OT - Diagnosis OT - Epidemiology OT - Equine coronavirus OT - Treatment EDAT- 2018/02/13 06:00 MHDA- 2018/08/15 06:00 PMCR- 2017/11/20 CRDT- 2018/02/13 06:00 PHST- 2017/04/07 00:00 [received] PHST- 2017/11/07 00:00 [revised] PHST- 2017/11/15 00:00 [accepted] PHST- 2018/02/13 06:00 [entrez] PHST- 2018/02/13 06:00 [pubmed] PHST- 2018/08/15 06:00 [medline] PHST- 2017/11/20 00:00 [pmc-release] AID - S1090-0233(17)30222-8 [pii] AID - 10.1016/j.tvjl.2017.11.004 [doi] PST - ppublish SO - Vet J. 2018 Jan;231:13-18. doi: 10.1016/j.tvjl.2017.11.004. Epub 2017 Nov 20. PMID- 28279346 OWN - NLM STAT- MEDLINE DCOM- 20170829 LR - 20200930 IS - 1934-6069 (Electronic) IS - 1931-3128 (Print) IS - 1931-3128 (Linking) VI - 21 IP - 3 DP - 2017 Mar 8 TI - Betacoronavirus Adaptation to Humans Involved Progressive Loss of Hemagglutinin-Esterase Lectin Activity. PG - 356-366 LID - S1931-3128(17)30070-7 [pii] LID - 10.1016/j.chom.2017.02.008 [doi] AB - Human beta1-coronavirus (β1CoV) OC43 emerged relatively recently through a single zoonotic introduction. Like related animal β1CoVs, OC43 uses 9-O-acetylated sialic acid as receptor determinant. β1CoV receptor binding is typically controlled by attachment/fusion spike protein S and receptor-binding/receptor-destroying hemagglutinin-esterase protein HE. We show that following OC43's introduction into humans, HE-mediated receptor binding was selected against and ultimately lost through progressive accumulation of mutations in the HE lectin domain. Consequently, virion-associated receptor-destroying activity toward multivalent glycoconjugates was reduced and altered such that some clustered receptor populations are no longer cleaved. Loss of HE lectin function was also observed for another respiratory human coronavirus, HKU1. This thus appears to be an adaptation to the sialoglycome of the human respiratory tract and for replication in human airways. The findings suggest that the dynamics of virion-glycan interactions contribute to host tropism. Our observations are relevant also to other human respiratory viruses of zoonotic origin, particularly influenza A virus. CI - Copyright © 2017 Elsevier Inc. All rights reserved. FAU - Bakkers, Mark J G AU - Bakkers MJ AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - Lang, Yifei AU - Lang Y AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - Feitsma, Louris J AU - Feitsma LJ AD - Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Faculty of Sciences, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - Hulswit, Ruben J G AU - Hulswit RJ AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - de Poot, Stefanie A H AU - de Poot SA AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - van Vliet, Arno L W AU - van Vliet AL AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - Margine, Irina AU - Margine I AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - de Groot-Mijnes, Jolanda D F AU - de Groot-Mijnes JD AD - Department of Medical Microbiology, University Medical Center Utrecht, 3584 CX Utrecht, the Netherlands. FAU - van Kuppeveld, Frank J M AU - van Kuppeveld FJ AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - Langereis, Martijn A AU - Langereis MA AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - Huizinga, Eric G AU - Huizinga EG AD - Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Faculty of Sciences, Utrecht University, 3584 CH Utrecht, the Netherlands. FAU - de Groot, Raoul J AU - de Groot RJ AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, the Netherlands. Electronic address: r.j.degroot@uu.nl. LA - eng PT - Journal Article PL - United States TA - Cell Host Microbe JT - Cell host & microbe JID - 101302316 RN - 0 (Hemagglutinins, Viral) RN - 0 (Lectins) RN - 0 (Receptors, Virus) RN - 0 (Viral Fusion Proteins) RN - 0 (hemagglutinin esterase) SB - IM MH - *Adaptation, Biological MH - Animals MH - Coronavirus OC43, Human/*genetics/physiology MH - Hemagglutinins, Viral/*genetics/*metabolism MH - Humans MH - Lectins/*genetics/*metabolism MH - Mutation MH - Protein Binding MH - Receptors, Virus/metabolism MH - Viral Fusion Proteins/*genetics/*metabolism MH - *Virus Attachment PMC - PMC7104930 EDAT- 2017/03/11 06:00 MHDA- 2017/08/30 06:00 PMCR- 2017/03/08 CRDT- 2017/03/11 06:00 PHST- 2016/10/12 00:00 [received] PHST- 2017/01/07 00:00 [revised] PHST- 2017/02/10 00:00 [accepted] PHST- 2017/03/11 06:00 [entrez] PHST- 2017/03/11 06:00 [pubmed] PHST- 2017/08/30 06:00 [medline] PHST- 2017/03/08 00:00 [pmc-release] AID - S1931-3128(17)30070-7 [pii] AID - 10.1016/j.chom.2017.02.008 [doi] PST - ppublish SO - Cell Host Microbe. 2017 Mar 8;21(3):356-366. doi: 10.1016/j.chom.2017.02.008. PMID- 28993568 OWN - NLM STAT- MEDLINE DCOM- 20180917 LR - 20240327 IS - 1347-7439 (Electronic) IS - 0916-7250 (Print) IS - 0916-7250 (Linking) VI - 79 IP - 11 DP - 2017 Nov 17 TI - Antibody response to equine coronavirus in horses inoculated with a bovine coronavirus vaccine. PG - 1889-1891 LID - 10.1292/jvms.17-0414 [doi] AB - A vaccine for equine coronavirus (ECoV) is so far unavailable. Bovine coronavirus (BCoV) is antigenically related to ECoV; it is therefore possible that BCoV vaccine will induce antibodies against ECoV in horses. This study investigated antibody response to ECoV in horses inoculated with BCoV vaccine. Virus neutralization tests showed that antibody titers against ECoV increased in all six horses tested at 14 days post inoculation, although the antibody titers were lower against ECoV than against BCoV. This study showed that BCoV vaccine provides horses with antibodies against ECoV to some extent. It is unclear whether antibodies provided by BCoV vaccine are effective against ECoV, and therefore ECoV challenge studies are needed to evaluate efficacy of the vaccine in the future. FAU - Nemoto, Manabu AU - Nemoto M AD - Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan. FAU - Kanno, Toru AU - Kanno T AD - Hokkaido Research Station, National Institute of Animal Health, 4 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-0045, Japan. FAU - Bannai, Hiroshi AU - Bannai H AD - Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan. FAU - Tsujimura, Koji AU - Tsujimura K AD - Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan. FAU - Yamanaka, Takashi AU - Yamanaka T AD - Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan. FAU - Kokado, Hiroshi AU - Kokado H AD - Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan. LA - eng PT - Journal Article DEP - 20171006 PL - Japan TA - J Vet Med Sci JT - The Journal of veterinary medical science JID - 9105360 RN - 0 (Antibodies, Viral) RN - 0 (Vaccines) SB - IM MH - Animals MH - Antibodies, Viral MH - Antibody Formation MH - Betacoronavirus 1/*immunology MH - Coronavirus Infections/prevention & control/*veterinary MH - Coronavirus, Bovine/*immunology MH - Horse Diseases/*immunology/prevention & control/*virology MH - Horses MH - Vaccination/*veterinary MH - Vaccines/immunology PMC - PMC5709570 OTO - NOTNLM OT - bovine coronavirus OT - equine coronavirus OT - horses OT - vaccine EDAT- 2017/10/11 06:00 MHDA- 2018/09/18 06:00 PMCR- 2017/11/01 CRDT- 2017/10/11 06:00 PHST- 2017/10/11 06:00 [pubmed] PHST- 2018/09/18 06:00 [medline] PHST- 2017/10/11 06:00 [entrez] PHST- 2017/11/01 00:00 [pmc-release] AID - 17-0414 [pii] AID - 10.1292/jvms.17-0414 [doi] PST - ppublish SO - J Vet Med Sci. 2017 Nov 17;79(11):1889-1891. doi: 10.1292/jvms.17-0414. Epub 2017 Oct 6. PMID- 25810418 OWN - NLM STAT- MEDLINE DCOM- 20150421 LR - 20220311 IS - 1098-6618 (Electronic) IS - 0893-8512 (Print) IS - 0893-8512 (Linking) VI - 28 IP - 2 DP - 2015 Apr TI - Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease. PG - 465-522 LID - 10.1128/CMR.00102-14 [doi] AB - The source of the severe acute respiratory syndrome (SARS) epidemic was traced to wildlife market civets and ultimately to bats. Subsequent hunting for novel coronaviruses (CoVs) led to the discovery of two additional human and over 40 animal CoVs, including the prototype lineage C betacoronaviruses, Tylonycteris bat CoV HKU4 and Pipistrellus bat CoV HKU5; these are phylogenetically closely related to the Middle East respiratory syndrome (MERS) CoV, which has affected more than 1,000 patients with over 35% fatality since its emergence in 2012. All primary cases of MERS are epidemiologically linked to the Middle East. Some of these patients had contacted camels which shed virus and/or had positive serology. Most secondary cases are related to health care-associated clusters. The disease is especially severe in elderly men with comorbidities. Clinical severity may be related to MERS-CoV's ability to infect a broad range of cells with DPP4 expression, evade the host innate immune response, and induce cytokine dysregulation. Reverse transcription-PCR on respiratory and/or extrapulmonary specimens rapidly establishes diagnosis. Supportive treatment with extracorporeal membrane oxygenation and dialysis is often required in patients with organ failure. Antivirals with potent in vitro activities include neutralizing monoclonal antibodies, antiviral peptides, interferons, mycophenolic acid, and lopinavir. They should be evaluated in suitable animal models before clinical trials. Developing an effective camel MERS-CoV vaccine and implementing appropriate infection control measures may control the continuing epidemic. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Chan, Jasper F W AU - Chan JF AD - State Key Laboratory of Emerging Infectious Diseases and Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Lau, Susanna K P AU - Lau SK AD - State Key Laboratory of Emerging Infectious Diseases and Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - To, Kelvin K W AU - To KK AD - State Key Laboratory of Emerging Infectious Diseases and Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Cheng, Vincent C C AU - Cheng VC AD - Carol Yu Centre for Infection, Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Woo, Patrick C Y AU - Woo PC AD - State Key Laboratory of Emerging Infectious Diseases and Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory of Emerging Infectious Diseases and Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review PL - United States TA - Clin Microbiol Rev JT - Clinical microbiology reviews JID - 8807282 RN - 0 (Antiviral Agents) SB - IM MH - Animals MH - Antiviral Agents/therapeutic use MH - Coronavirus Infections/epidemiology/pathology/therapy/transmission/*virology MH - Disease Models, Animal MH - Humans MH - Infection Control MH - Middle East Respiratory Syndrome Coronavirus/classification/*physiology MH - Zoonoses/epidemiology/pathology/therapy/transmission/*virology PMC - PMC4402954 EDAT- 2015/03/27 06:00 MHDA- 2015/04/22 06:00 PMCR- 2016/04/01 CRDT- 2015/03/27 06:00 PHST- 2015/03/27 06:00 [entrez] PHST- 2015/03/27 06:00 [pubmed] PHST- 2015/04/22 06:00 [medline] PHST- 2016/04/01 00:00 [pmc-release] AID - 28/2/465 [pii] AID - 00102-14 [pii] AID - 10.1128/CMR.00102-14 [doi] PST - ppublish SO - Clin Microbiol Rev. 2015 Apr;28(2):465-522. doi: 10.1128/CMR.00102-14. PMID- 31383304 OWN - NLM STAT- MEDLINE DCOM- 20191210 LR - 20211204 IS - 1873-2542 (Electronic) IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 235 DP - 2019 Aug TI - Porcine epidemic diarrhea virus ORF3 protein causes endoplasmic reticulum stress to facilitate autophagy. PG - 209-219 LID - S0378-1135(19)30528-0 [pii] LID - 10.1016/j.vetmic.2019.07.005 [doi] AB - Porcine epidemic diarrhea virus (PEDV), the causative agent of PED, is an enveloped, positive-stranded RNA virus in the genus Alphacoronavirus, family Coronaviridae, order Nidovirales. PEDV non-structural accessory protein ORF3 is an ion channel related to viral infectivity and pathogenicity. Our previous study showed that PEDV ORF3 has expression characteristic of aggregation in cytoplasm, but its biological function remains elusive. Thus in this study, we initiated the construction of various vectors to express ORF3, and found ORF3 localized in the cytoplasm in the aggregation manner. Subsequently, confocal microscopy analysis showed that the aggregated ORF3 localized in endoplasmic reticulum (ER) to trigger ER stress response via up-regulation of GRP78 protein expression and activation of PERK-eIF2α signaling pathway. In addition, our results showed that PEDV ORF3 could induce the autophagy through inducing conversion of LC3-I to LC3-II, but couldn't influence the apoptosis. In contrast, conversion of LC3-I/LC3-II could be significantly inhibited by 4-PBA, an ER stress inhibitor, indicating that ORF3-induced autophagy is dependent on ER stress response. This work not only provides some new findings for the biological function of the PEDV ORF3 protein, but also help us for the further understanding the molecular interaction between PEDV ORF3 protein and cells. CI - Copyright © 2019 Elsevier B.V. All rights reserved. FAU - Zou, Dehua AU - Zou D AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Xu, Jiaxin AU - Xu J AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Duan, Xulai AU - Duan X AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Xu, Xin AU - Xu X AD - Branch of Animal Husbandry and Veterinary of HeiLongJiang Academy of Agricultural Sciences, Qiqihar, 161005, China. FAU - Li, Pengfei AU - Li P AD - Department of Nephrology, The Fifth Affiliated Hospital of Harbin Medical University, Daqing 163319, China. FAU - Cheng, Lixin AU - Cheng L AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Zheng, Liang AU - Zheng L AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Li, Xingzhi AU - Li X AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Zhang, Yating AU - Zhang Y AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Wang, Xianhe AU - Wang X AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Wu, Xuening AU - Wu X AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Shen, Yujiang AU - Shen Y AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Yao, Xiangyu AU - Yao X AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Wei, Jiaqi AU - Wei J AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Yao, Lili AU - Yao L AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Li, Liyang AU - Li L AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Song, Baifen AU - Song B AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Ma, Jinzhu AU - Ma J AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Liu, Xinyang AU - Liu X AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Wu, Zhijun AU - Wu Z AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. FAU - Zhang, Hua AU - Zhang H AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. Electronic address: byndhzh@163.com. FAU - Cao, Hongwei AU - Cao H AD - College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; Biotechnology Center, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. Electronic address: byndcaohongwei@163.com. LA - eng PT - Journal Article DEP - 20190708 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Endoplasmic Reticulum Chaperone BiP) RN - 0 (HSPA5 protein, human) RN - 0 (Heat-Shock Proteins) RN - 0 (Viral Proteins) SB - IM MH - Animals MH - *Autophagy MH - Chlorocebus aethiops MH - Endoplasmic Reticulum/pathology/*virology MH - Endoplasmic Reticulum Chaperone BiP MH - *Endoplasmic Reticulum Stress MH - Genetic Vectors MH - HEK293 Cells MH - HeLa Cells MH - Heat-Shock Proteins/genetics MH - Humans MH - *Open Reading Frames MH - Porcine epidemic diarrhea virus/*pathogenicity MH - Signal Transduction MH - Swine MH - Vero Cells MH - Viral Proteins/*genetics MH - Virus Replication PMC - PMC7117398 OTO - NOTNLM OT - Accessory protein ORF3 OT - Apoptosis OT - Autophagy OT - Endoplasmic reticulum stress OT - Porcine epidemic diarrhea virus EDAT- 2019/08/07 06:00 MHDA- 2019/12/18 06:00 PMCR- 2019/07/08 CRDT- 2019/08/07 06:00 PHST- 2019/04/29 00:00 [received] PHST- 2019/07/05 00:00 [revised] PHST- 2019/07/06 00:00 [accepted] PHST- 2019/08/07 06:00 [entrez] PHST- 2019/08/07 06:00 [pubmed] PHST- 2019/12/18 06:00 [medline] PHST- 2019/07/08 00:00 [pmc-release] AID - S0378-1135(19)30528-0 [pii] AID - 10.1016/j.vetmic.2019.07.005 [doi] PST - ppublish SO - Vet Microbiol. 2019 Aug;235:209-219. doi: 10.1016/j.vetmic.2019.07.005. Epub 2019 Jul 8. PMID- 27274850 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20160609 LR - 20200929 IS - 2052-2975 (Print) IS - 2052-2975 (Electronic) IS - 2052-2975 (Linking) VI - 11 DP - 2016 May TI - First genome sequences of buffalo coronavirus from water buffaloes in Bangladesh. PG - 54-6 LID - 10.1016/j.nmni.2016.02.011 [doi] AB - We report the complete genome sequences of a buffalo coronavirus (BufCoV HKU26) detected from the faecal samples of two domestic water buffaloes (Bubalus bubalis) in Bangladesh. They possessed 98-99% nucleotide identities to bovine coronavirus (BCoV) genomes, supporting BufCoV HKU26 as a member of Betacoronavirus 1. Nevertheless, BufCoV HKU26 possessed distinct accessory proteins between spike and envelope compared to BCoV. Sugar-binding residues in the N-terminal domain of S protein in BCoV are conserved in BufCoV HKU26. FAU - Lau, S K P AU - Lau SK AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China. FAU - Tsang, A K L AU - Tsang AK AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Shakeel Ahmed, S AU - Shakeel Ahmed S AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Mahbub Alam, M AU - Mahbub Alam M AD - United Hospital Limited, Bangladesh. FAU - Ahmed, Z AU - Ahmed Z AD - BRAC Dairy and Food Project, BRAC Enterprises, Dhaka, Bangladesh. FAU - Wong, P-C AU - Wong PC AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Yuen, K-Y AU - Yuen KY AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China. FAU - Woo, P C Y AU - Woo PC AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China. LA - eng PT - Journal Article DEP - 20160303 PL - England TA - New Microbes New Infect JT - New microbes and new infections JID - 101624750 PMC - PMC4879238 OTO - NOTNLM OT - Coronavirus OT - water buffalo EDAT- 2016/06/09 06:00 MHDA- 2016/06/09 06:01 PMCR- 2016/03/03 CRDT- 2016/06/09 06:00 PHST- 2016/01/29 00:00 [received] PHST- 2016/02/23 00:00 [accepted] PHST- 2016/06/09 06:00 [entrez] PHST- 2016/06/09 06:00 [pubmed] PHST- 2016/06/09 06:01 [medline] PHST- 2016/03/03 00:00 [pmc-release] AID - S2052-2975(16)00028-7 [pii] AID - 10.1016/j.nmni.2016.02.011 [doi] PST - epublish SO - New Microbes New Infect. 2016 Mar 3;11:54-6. doi: 10.1016/j.nmni.2016.02.011. eCollection 2016 May. PMID- 27644155 OWN - NLM STAT- MEDLINE DCOM- 20171023 LR - 20221207 IS - 2164-554X (Electronic) IS - 2164-5515 (Print) IS - 2164-5515 (Linking) VI - 13 IP - 1 DP - 2017 Jan 2 TI - Understanding bat SARS-like coronaviruses for the preparation of future coronavirus outbreaks - Implications for coronavirus vaccine development. PG - 186-189 LID - 10.1080/21645515.2016.1228500 [doi] AB - The severe acute respiratory syndrome coronavirus (SARS-CoV) first emerged in 2003, causing the SARS epidemic which resulted in a 10% fatality rate. The advancements in metagenomic techniques have allowed the identification of SARS-like coronaviruses (SL-CoVs) sequences that share high homology to the human SARS-CoV epidemic strains from wildlife bats, presenting concrete evidence that bats are the origin and natural reservoir of SARS-CoV. The application of reverse genetics further enabled that characterization of these bat CoVs and the prediction of their potential to cause disease in humans. The knowledge gained from such studies is valuable in the surveillance and preparation of a possible future outbreak caused by a spill-over of these bat SL-CoVs. FAU - Ng, Oi-Wing AU - Ng OW AD - a Department of Microbiology and Immunology , Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore , Singapore. FAU - Tan, Yee-Joo AU - Tan YJ AD - a Department of Microbiology and Immunology , Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore , Singapore. AD - b Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research) , Singapore. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160920 PL - United States TA - Hum Vaccin Immunother JT - Human vaccines & immunotherapeutics JID - 101572652 RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Chiroptera/*virology MH - *Disease Outbreaks MH - High-Throughput Nucleotide Sequencing MH - Humans MH - Reverse Genetics MH - Severe acute respiratory syndrome-related coronavirus/*genetics/immunology/isolation & purification/pathogenicity MH - Severe Acute Respiratory Syndrome/*epidemiology/*virology MH - Viral Vaccines/*immunology/*isolation & purification PMC - PMC5287300 OTO - NOTNLM OT - Bats OT - Emerging and re-emerging virus OT - SARS-like coronavirus OT - Severe acute respiratory syndrome coronavirus OT - Zoonotic virus EDAT- 2016/09/21 06:00 MHDA- 2017/10/24 06:00 PMCR- 2017/09/20 CRDT- 2016/09/21 06:00 PHST- 2016/09/21 06:00 [pubmed] PHST- 2017/10/24 06:00 [medline] PHST- 2016/09/21 06:00 [entrez] PHST- 2017/09/20 00:00 [pmc-release] AID - 1228500 [pii] AID - 10.1080/21645515.2016.1228500 [doi] PST - ppublish SO - Hum Vaccin Immunother. 2017 Jan 2;13(1):186-189. doi: 10.1080/21645515.2016.1228500. Epub 2016 Sep 20. PMID- 25736566 OWN - NLM STAT- MEDLINE DCOM- 20160316 LR - 20181113 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 206 DP - 2015 Aug 3 TI - The structure and functions of coronavirus genomic 3' and 5' ends. PG - 120-33 LID - S0168-1702(15)00115-X [pii] LID - 10.1016/j.virusres.2015.02.025 [doi] AB - Coronaviruses (CoVs) are an important cause of illness in humans and animals. Most human coronaviruses commonly cause relatively mild respiratory illnesses; however two zoonotic coronaviruses, SARS-CoV and MERS-CoV, can cause severe illness and death. Investigations over the past 35 years have illuminated many aspects of coronavirus replication. The focus of this review is the functional analysis of conserved RNA secondary structures in the 5' and 3' of the betacoronavirus genomes. The 5' 350 nucleotides folds into a set of RNA secondary structures which are well conserved, and reverse genetic studies indicate that these structures play an important role in the discontinuous synthesis of subgenomic RNAs in the betacoronaviruses. These cis-acting elements extend 3' of the 5'UTR into ORF1a. The 3'UTR is similarly conserved and contains all of the cis-acting sequences necessary for viral replication. Two competing conformations near the 5' end of the 3'UTR have been shown to make up a potential molecular switch. There is some evidence that an association between the 3' and 5'UTRs is necessary for subgenomic RNA synthesis, but the basis for this association is not yet clear. A number of host RNA proteins have been shown to bind to the 5' and 3' cis-acting regions, but the significance of these in viral replication is not clear. Two viral proteins have been identified as binding to the 5' cis-acting region, nsp1 and N protein. A genetic interaction between nsp8 and nsp9 and the region of the 3'UTR that contains the putative molecular switch suggests that these two proteins bind to this region. CI - Copyright © 2015 Elsevier B.V. All rights reserved. FAU - Yang, Dong AU - Yang D AD - Department of Microbiology, Immunology & Biochemistry, The University of Tennessee Health Science Center College of Medicine, Memphis, TN 38163, USA. FAU - Leibowitz, Julian L AU - Leibowitz JL AD - Department of Microbial Pathogenesis and Immunology, Texas A&M University, College of Medicine, College Station, TX 77843-1114, USA. Electronic address: jleibowitz@tamu.edu. LA - eng GR - R01 AI067416/AI/NIAID NIH HHS/United States GR - 1R01 AI067416/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20150228 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (3' Untranslated Regions) RN - 0 (5' Untranslated Regions) RN - 0 (RNA, Viral) RN - 0 (RNA-Binding Proteins) SB - IM MH - *3' Untranslated Regions MH - *5' Untranslated Regions MH - Animals MH - Conserved Sequence MH - Coronavirus/genetics/*physiology MH - *Genome, Viral MH - *Host-Pathogen Interactions MH - Humans MH - Models, Biological MH - Nucleic Acid Conformation MH - Promoter Regions, Genetic MH - RNA, Viral/chemistry/genetics/*metabolism MH - RNA-Binding Proteins/metabolism MH - Transcription, Genetic MH - *Virus Replication PMC - PMC4476908 MID - NIHMS668961 OTO - NOTNLM OT - Coronaviruses OT - RNA binding proteins OT - RNA secondary structure OT - Virus replication OT - cis-Acting sequences EDAT- 2015/03/05 06:00 MHDA- 2016/03/17 06:00 PMCR- 2015/02/28 CRDT- 2015/03/05 06:00 PHST- 2014/11/01 00:00 [received] PHST- 2015/02/22 00:00 [revised] PHST- 2015/02/23 00:00 [accepted] PHST- 2015/03/05 06:00 [entrez] PHST- 2015/03/05 06:00 [pubmed] PHST- 2016/03/17 06:00 [medline] PHST- 2015/02/28 00:00 [pmc-release] AID - S0168-1702(15)00115-X [pii] AID - 10.1016/j.virusres.2015.02.025 [doi] PST - ppublish SO - Virus Res. 2015 Aug 3;206:120-33. doi: 10.1016/j.virusres.2015.02.025. Epub 2015 Feb 28. PMID- 29219990 OWN - NLM STAT- MEDLINE DCOM- 20180321 LR - 20230605 IS - 1476-4687 (Electronic) IS - 0028-0836 (Linking) VI - 552 IP - 7683 DP - 2017 Dec 7 TI - Bat cave solves mystery of deadly SARS virus - and suggests new outbreak could occur. PG - 15-16 LID - 10.1038/d41586-017-07766-9 [doi] FAU - Cyranoski, David AU - Cyranoski D LA - eng PT - News PL - England TA - Nature JT - Nature JID - 0410462 SB - IM CIN - Nature. 2018 Jan 18;553(7688):281. doi: 10.1038/d41586-018-00603-7. PMID: 29345663 MH - Animals MH - *Caves MH - China/epidemiology MH - Chiroptera/*virology MH - *Disease Outbreaks/veterinary MH - Evolution, Molecular MH - Humans MH - Molecular Epidemiology MH - Severe acute respiratory syndrome-related coronavirus/*genetics/isolation & purification/pathogenicity MH - Severe Acute Respiratory Syndrome/*epidemiology/transmission/veterinary/*virology MH - Viverridae/virology MH - Zoonoses/epidemiology/mortality/transmission/*virology EDAT- 2017/12/09 06:00 MHDA- 2018/03/22 06:00 CRDT- 2017/12/09 06:00 PHST- 2017/12/09 06:00 [entrez] PHST- 2017/12/09 06:00 [pubmed] PHST- 2018/03/22 06:00 [medline] AID - d41586-017-07766-9 [pii] AID - 10.1038/d41586-017-07766-9 [doi] PST - ppublish SO - Nature. 2017 Dec 7;552(7683):15-16. doi: 10.1038/d41586-017-07766-9. PMID- 25720483 OWN - NLM STAT- MEDLINE DCOM- 20151113 LR - 20200407 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 1282 DP - 2015 TI - A field-proven yeast two-hybrid protocol used to identify coronavirus-host protein-protein interactions. PG - 213-29 LID - 10.1007/978-1-4939-2438-7_18 [doi] AB - Over the last 2 decades, yeast two-hybrid became an invaluable technique to decipher protein-protein interaction networks. In the field of virology, it has proven instrumental to identify virus-host interactions that are involved in viral embezzlement of cellular functions and inhibition of immune mechanisms. Here, we present a yeast two-hybrid protocol that has been used in our laboratory since 2006 to search for cellular partners of more than 300 viral proteins. Our aim was to develop a robust and straightforward pipeline, which minimizes false-positive interactions with a decent coverage of target cDNA libraries, and only requires a minimum of equipment. We also discuss reasons that motivated our technical choices and compromises that had to be made. This protocol has been used to screen most non-structural proteins of murine hepatitis virus (MHV), a member of betacoronavirus genus, against a mouse brain cDNA library. Typical results were obtained and are presented in this report. FAU - Vidalain, Pierre-Olivier AU - Vidalain PO AD - Unité de Génomique Virale etVaccination, Institut Pasteur, Paris, France, pierre-olivier.vidalain@pasteur.fr. FAU - Jacob, Yves AU - Jacob Y FAU - Hagemeijer, Marne C AU - Hagemeijer MC FAU - Jones, Louis M AU - Jones LM FAU - Neveu, Grégory AU - Neveu G FAU - Roussarie, Jean-Pierre AU - Roussarie JP FAU - Rottier, Peter J M AU - Rottier PJ FAU - Tangy, Frédéric AU - Tangy F FAU - de Haan, Cornelis A M AU - de Haan CA LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 0 (Nerve Tissue Proteins) RN - 0 (Viral Proteins) SB - IM MH - Animals MH - Host-Pathogen Interactions MH - Mice MH - Murine hepatitis virus/*physiology MH - Nerve Tissue Proteins/*metabolism MH - *Two-Hybrid System Techniques MH - Viral Proteins/*metabolism MH - Virus Attachment PMC - PMC7121825 EDAT- 2015/02/28 06:00 MHDA- 2015/11/14 06:00 PMCR- 2020/04/03 CRDT- 2015/02/28 06:00 PHST- 2015/02/28 06:00 [entrez] PHST- 2015/02/28 06:00 [pubmed] PHST- 2015/11/14 06:00 [medline] PHST- 2020/04/03 00:00 [pmc-release] AID - 18 [pii] AID - 10.1007/978-1-4939-2438-7_18 [doi] PST - ppublish SO - Methods Mol Biol. 2015;1282:213-29. doi: 10.1007/978-1-4939-2438-7_18. PMID- 28628449 OWN - NLM STAT- MEDLINE DCOM- 20180326 LR - 20181113 IS - 1080-6059 (Electronic) IS - 1080-6040 (Print) IS - 1080-6040 (Linking) VI - 23 IP - 7 DP - 2017 Jul TI - Porcine Hemagglutinating Encephalomyelitis Virus and Respiratory Disease in Exhibition Swine, Michigan, USA, 2015. PG - 1168-1171 LID - 10.3201/eid2307.170019 [doi] AB - Acute outbreaks of respiratory disease in swine at agricultural fairs in Michigan, USA, in 2015 raised concern for potential human exposure to influenza A virus. Testing ruled out influenza A virus and identified porcine hemagglutinating encephalomyelitis virus as the cause of influenza-like illness in the affected swine. FAU - Lorbach, Joshua N AU - Lorbach JN FAU - Wang, Leyi AU - Wang L FAU - Nolting, Jacqueline M AU - Nolting JM FAU - Benjamin, Madonna G AU - Benjamin MG FAU - Killian, Mary Lea AU - Killian ML FAU - Zhang, Yan AU - Zhang Y FAU - Bowman, Andrew S AU - Bowman AS LA - eng GR - HHSN272201400006C/AI/NIAID NIH HHS/United States PT - Historical Article PT - Journal Article PL - United States TA - Emerg Infect Dis JT - Emerging infectious diseases JID - 9508155 SB - IM MH - Animals MH - Betacoronavirus 1/*classification/*genetics MH - Coronavirus Infections/*veterinary MH - Disease Outbreaks MH - Genome, Viral MH - Genotype MH - History, 21st Century MH - Michigan/epidemiology MH - Phylogeny MH - Respiratory Tract Diseases/*veterinary MH - Swine MH - Swine Diseases/*epidemiology/history/*virology PMC - PMC5512476 OTO - NOTNLM OT - Indiana OT - Michigan OT - Ohio OT - United States OT - coronavirus OT - differential diagnosis OT - disease outbreaks OT - exhibition swine OT - influenza A virus OT - porcine hemagglutinating encephalomyelitis OT - porcine hemagglutinating encephalomyelitis virus OT - respiratory infections OT - swine OT - viruses EDAT- 2017/06/20 06:00 MHDA- 2018/03/27 06:00 PMCR- 2017/07/01 CRDT- 2017/06/20 06:00 PHST- 2017/06/20 06:00 [entrez] PHST- 2017/06/20 06:00 [pubmed] PHST- 2018/03/27 06:00 [medline] PHST- 2017/07/01 00:00 [pmc-release] AID - 17-0019 [pii] AID - 10.3201/eid2307.170019 [doi] PST - ppublish SO - Emerg Infect Dis. 2017 Jul;23(7):1168-1171. doi: 10.3201/eid2307.170019. PMID- 29083948 OWN - NLM STAT- MEDLINE DCOM- 20180709 LR - 20231112 IS - 2164-554X (Electronic) IS - 2164-5515 (Print) IS - 2164-5515 (Linking) VI - 13 IP - 12 DP - 2017 Dec 2 TI - Chimpanzee adenoviral vectors as vaccines for outbreak pathogens. PG - 3020-3032 LID - 10.1080/21645515.2017.1383575 [doi] AB - The 2014-15 Ebola outbreak in West Africa highlighted the potential for large disease outbreaks caused by emerging pathogens and has generated considerable focus on preparedness for future epidemics. Here we discuss drivers, strategies and practical considerations for developing vaccines against outbreak pathogens. Chimpanzee adenoviral (ChAd) vectors have been developed as vaccine candidates for multiple infectious diseases and prostate cancer. ChAd vectors are safe and induce antigen-specific cellular and humoral immunity in all age groups, as well as circumventing the problem of pre-existing immunity encountered with human Ad vectors. For these reasons, such viral vectors provide an attractive platform for stockpiling vaccines for emergency deployment in response to a threatened outbreak of an emerging pathogen. Work is already underway to develop vaccines against a number of other outbreak pathogens and we will also review progress on these approaches here, particularly for Lassa fever, Nipah and MERS. FAU - Ewer, Katie AU - Ewer K AUID- ORCID: 0000-0001-9827-9836 AD - a The Jenner Institute, University of Oxford , Old Road Campus Research Building, Headington , Oxford , UK. FAU - Sebastian, Sarah AU - Sebastian S AUID- ORCID: 0000-0001-7918-4321 AD - a The Jenner Institute, University of Oxford , Old Road Campus Research Building, Headington , Oxford , UK. FAU - Spencer, Alexandra J AU - Spencer AJ AUID- ORCID: 0000-0001-7958-6961 AD - a The Jenner Institute, University of Oxford , Old Road Campus Research Building, Headington , Oxford , UK. FAU - Gilbert, Sarah AU - Gilbert S AUID- ORCID: 0000-0002-6823-9750 AD - a The Jenner Institute, University of Oxford , Old Road Campus Research Building, Headington , Oxford , UK. FAU - Hill, Adrian V S AU - Hill AVS AUID- ORCID: 0000-0003-0900-9629 AD - a The Jenner Institute, University of Oxford , Old Road Campus Research Building, Headington , Oxford , UK. FAU - Lambe, Teresa AU - Lambe T AUID- ORCID: 0000-0001-7711-897X AD - a The Jenner Institute, University of Oxford , Old Road Campus Research Building, Headington , Oxford , UK. LA - eng PT - Journal Article PT - Review DEP - 20171030 PL - United States TA - Hum Vaccin Immunother JT - Human vaccines & immunotherapeutics JID - 101572652 RN - 0 (Drug Carriers) RN - 0 (Vaccines) SB - IM MH - Adenoviridae/*genetics/*isolation & purification MH - Animals MH - Betacoronavirus MH - *Drug Carriers MH - Drug Discovery/*methods MH - *Genetic Vectors MH - Humans MH - Pan troglodytes/*virology MH - Vaccines/genetics/*immunology PMC - PMC5718829 OTO - NOTNLM OT - Lassa fever OT - MERS Co-V OT - Nipah OT - Vaccines OT - viral vectors EDAT- 2017/10/31 06:00 MHDA- 2018/07/10 06:00 PMCR- 2017/10/30 CRDT- 2017/10/31 06:00 PHST- 2017/10/31 06:00 [pubmed] PHST- 2018/07/10 06:00 [medline] PHST- 2017/10/31 06:00 [entrez] PHST- 2017/10/30 00:00 [pmc-release] AID - 1383575 [pii] AID - 10.1080/21645515.2017.1383575 [doi] PST - ppublish SO - Hum Vaccin Immunother. 2017 Dec 2;13(12):3020-3032. doi: 10.1080/21645515.2017.1383575. Epub 2017 Oct 30. PMID- 26689940 OWN - NLM STAT- MEDLINE DCOM- 20161006 LR - 20220311 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 12 DP - 2015 Dec 22 TI - Bat origin of human coronaviruses. PG - 221 LID - 10.1186/s12985-015-0422-1 [doi] LID - 221 AB - Bats have been recognized as the natural reservoirs of a large variety of viruses. Special attention has been paid to bat coronaviruses as the two emerging coronaviruses which have caused unexpected human disease outbreaks in the 21st century, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Middle East Respiratory Syndrome Coronavirus (MERS-CoV), are suggested to be originated from bats. Various species of horseshoe bats in China have been found to harbor genetically diverse SARS-like coronaviruses. Some strains are highly similar to SARS-CoV even in the spike protein and are able to use the same receptor as SARS-CoV for cell entry. On the other hand, diverse coronaviruses phylogenetically related to MERS-CoV have been discovered worldwide in a wide range of bat species, some of which can be classified to the same coronavirus species as MERS-CoV. Coronaviruses genetically related to human coronavirus 229E and NL63 have been detected in bats as well. Moreover, intermediate hosts are believed to play an important role in the transmission and emergence of these coronaviruses from bats to humans. Understanding the bat origin of human coronaviruses is helpful for the prediction and prevention of another pandemic emergence in the future. FAU - Hu, Ben AU - Hu B AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Ge, Xingyi AU - Ge X AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Wang, Lin-Fa AU - Wang LF AD - Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore, 169857, Singapore. FAU - Shi, Zhengli AU - Shi Z AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. zlshi@wh.iov.cn. LA - eng GR - R01 AI110964/AI/NIAID NIH HHS/United States GR - NIAID R01AI110964/PHS HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20151222 PL - England TA - Virol J JT - Virology journal JID - 101231645 SB - IM MH - Animals MH - China MH - Chiroptera/*virology MH - Coronaviridae/*classification/genetics/*isolation & purification MH - Coronaviridae Infections/transmission/*veterinary/virology MH - Disease Reservoirs MH - Disease Transmission, Infectious MH - Disease Vectors MH - *Genetic Variation MH - Humans MH - Zoonoses/transmission/virology PMC - PMC4687304 EDAT- 2015/12/23 06:00 MHDA- 2016/10/08 06:00 PMCR- 2015/12/22 CRDT- 2015/12/23 06:00 PHST- 2015/09/16 00:00 [received] PHST- 2015/11/11 00:00 [accepted] PHST- 2015/12/23 06:00 [entrez] PHST- 2015/12/23 06:00 [pubmed] PHST- 2016/10/08 06:00 [medline] PHST- 2015/12/22 00:00 [pmc-release] AID - 10.1186/s12985-015-0422-1 [pii] AID - 422 [pii] AID - 10.1186/s12985-015-0422-1 [doi] PST - epublish SO - Virol J. 2015 Dec 22;12:221. doi: 10.1186/s12985-015-0422-1. PMID- 29331670 OWN - NLM STAT- MEDLINE DCOM- 20190110 LR - 20210109 IS - 1567-7257 (Electronic) IS - 1567-1348 (Print) IS - 1567-1348 (Linking) VI - 58 DP - 2018 Mar TI - Circulation of Alphacoronavirus, Betacoronavirus and Paramyxovirus in Hipposideros bat species in Zimbabwe. PG - 253-257 LID - S1567-1348(18)30007-8 [pii] LID - 10.1016/j.meegid.2018.01.007 [doi] AB - Bats carry a great diversity of zoonotic viruses with a high-impact on human health and livestock. Since the emergence of new coronaviruses and paramyxoviruses in humans (e.g. Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Nipah virus), numerous studies clearly established that bats can maintain some of these viruses. Improving our understanding on the role of bats in the epidemiology of the pathogens they harbour is necessary to prevent cross-species spill over along the wild/domestic/human gradient. In this study, we screened bat faecal samples for the presence of Coronavirus and Paramyxovirus in two caves frequently visited by local people to collect manure and/or to hunt bats in Zimbabwe. We amplified partial RNA-dependent RNA polymerase genes of Alpha and Betacoronavirus together with the partial polymerase gene of Paramyxovirus. Identified coronaviruses were related to pathogenic human strains and the paramyxovirus belonged to the recently described Jeilongvirus genus. Our results highlighted the importance of monitoring virus circulation in wildlife, especially bats, in the context of intense human-wildlife interfaces in order to strengthen prevention measures among local populations and to implement sentinel surveillance in sites with high zoonotic diseases transmission potential. CI - Copyright © 2018 Elsevier B.V. All rights reserved. FAU - Bourgarel, Mathieu AU - Bourgarel M AD - CIRAD, UMR ASTRE, RP-PCP, Harare, Zimbabwe; ASTRE, Univ. Montpellier, CIRAD, INRA, Montpellier, France. Electronic address: mathieu.bourgarel@cirad.com. FAU - Pfukenyi, Davies M AU - Pfukenyi DM AD - Faculty of Veterinary Science, University of Zimbabwe, P.O. Box MP167, Mt. Pleasant Harare, Zimbabwe. FAU - Boué, Vanina AU - Boué V AD - MIVEGEC, IRD, CNRS, Univ. Montpellier, Montpellier, France. FAU - Talignani, Loïc AU - Talignani L AD - MIVEGEC, IRD, CNRS, Univ. Montpellier, Montpellier, France. Electronic address: loic.talignani@ird.fr. FAU - Chiweshe, Ngoni AU - Chiweshe N AD - CIRAD, UMR ASTRE, RP-PCP, Harare, Zimbabwe. FAU - Diop, Fodé AU - Diop F AD - MIVEGEC, IRD, CNRS, Univ. Montpellier, Montpellier, France. Electronic address: fode.diop@ird.fr. FAU - Caron, Alexandre AU - Caron A AD - CIRAD, UMR ASTRE, RP-PCP, Harare, Zimbabwe; CIRAD, UMR ASTRE, RP-PCP, Maputo, Mozambique; Faculdade de Veterinária, Universidade Eduardo Mondlane, Maputo, Mozambique. Electronic address: alexandre.caron@cirad.fr. FAU - Matope, Gift AU - Matope G AD - Faculty of Veterinary Science, University of Zimbabwe, P.O. Box MP167, Mt. Pleasant Harare, Zimbabwe. FAU - Missé, Dorothée AU - Missé D AD - MIVEGEC, IRD, CNRS, Univ. Montpellier, Montpellier, France. Electronic address: dorothee.misse@ird.fr. FAU - Liégeois, Florian AU - Liégeois F AD - MIVEGEC, IRD, CNRS, Univ. Montpellier, Montpellier, France. Electronic address: florian.liegeois@ird.fr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180110 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 SB - IM MH - Alphacoronavirus/classification/*genetics MH - Animals MH - Betacoronavirus/classification/*genetics MH - Chiroptera/*virology MH - Communicable Diseases, Emerging/veterinary MH - Coronavirus Infections/*veterinary MH - Evolution, Molecular MH - Genetic Variation MH - Genome, Viral MH - Paramyxoviridae/classification/*genetics MH - Paramyxoviridae Infections/*veterinary MH - Phylogeny MH - Zimbabwe PMC - PMC7106086 OTO - NOTNLM OT - Bat OT - Coronavirus OT - Emerging infectious diseases OT - Paramyxovirus OT - Phylogeny OT - Zimbabwe COIS- We declare that we have no conflicts of interest. EDAT- 2018/01/15 06:00 MHDA- 2019/01/11 06:00 PMCR- 2018/01/10 CRDT- 2018/01/15 06:00 PHST- 2017/09/28 00:00 [received] PHST- 2018/01/08 00:00 [revised] PHST- 2018/01/09 00:00 [accepted] PHST- 2018/01/15 06:00 [pubmed] PHST- 2019/01/11 06:00 [medline] PHST- 2018/01/15 06:00 [entrez] PHST- 2018/01/10 00:00 [pmc-release] AID - S1567-1348(18)30007-8 [pii] AID - 10.1016/j.meegid.2018.01.007 [doi] PST - ppublish SO - Infect Genet Evol. 2018 Mar;58:253-257. doi: 10.1016/j.meegid.2018.01.007. Epub 2018 Jan 10. PMID- 25307890 OWN - NLM STAT- MEDLINE DCOM- 20150717 LR - 20211021 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 194 DP - 2014 Dec 19 TI - RNA structure analysis of alphacoronavirus terminal genome regions. PG - 76-89 LID - S0168-1702(14)00405-5 [pii] LID - 10.1016/j.virusres.2014.10.001 [doi] AB - Coronavirus genome replication is mediated by a multi-subunit protein complex that is comprised of more than a dozen virally encoded and several cellular proteins. Interactions of the viral replicase complex with cis-acting RNA elements located in the 5' and 3'-terminal genome regions ensure the specific replication of viral RNA. Over the past years, boundaries and structures of cis-acting RNA elements required for coronavirus genome replication have been extensively characterized in betacoronaviruses and, to a lesser extent, other coronavirus genera. Here, we review our current understanding of coronavirus cis-acting elements located in the terminal genome regions and use a combination of bioinformatic and RNA structure probing studies to identify and characterize putative cis-acting RNA elements in alphacoronaviruses. The study suggests significant RNA structure conservation among members of the genus Alphacoronavirus but also across genus boundaries. Overall, the conservation pattern identified for 5' and 3'-terminal RNA structural elements in the genomes of alpha- and betacoronaviruses is in agreement with the widely used replicase polyprotein-based classification of the Coronavirinae, suggesting co-evolution of the coronavirus replication machinery with cognate cis-acting RNA elements. CI - Copyright © 2014 Elsevier B.V. All rights reserved. FAU - Madhugiri, Ramakanth AU - Madhugiri R AD - Institute of Medical Virology, Justus Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, Germany. FAU - Fricke, Markus AU - Fricke M AD - Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743 Jena, Germany. FAU - Marz, Manja AU - Marz M AD - Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Leutragraben 1, 07743 Jena, Germany. FAU - Ziebuhr, John AU - Ziebuhr J AD - Institute of Medical Virology, Justus Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, Germany. Electronic address: john.ziebuhr@viro.med.uni-giessen.de. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20141013 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (RNA, Viral) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Base Sequence MH - Conserved Sequence MH - Coronavirus/chemistry/*genetics/physiology MH - *Genome, Viral MH - Models, Molecular MH - Molecular Sequence Data MH - *Nucleic Acid Conformation MH - Protein Binding MH - RNA, Viral/*chemistry/*genetics MH - RNA-Dependent RNA Polymerase/metabolism MH - Virus Replication PMC - PMC7114417 OTO - NOTNLM OT - Coronavirus OT - RNA structure OT - RNA virus OT - Replication OT - cis-Acting element EDAT- 2014/10/14 06:00 MHDA- 2015/07/18 06:00 PMCR- 2014/10/13 CRDT- 2014/10/14 06:00 PHST- 2014/08/26 00:00 [received] PHST- 2014/09/30 00:00 [revised] PHST- 2014/10/01 00:00 [accepted] PHST- 2014/10/14 06:00 [entrez] PHST- 2014/10/14 06:00 [pubmed] PHST- 2015/07/18 06:00 [medline] PHST- 2014/10/13 00:00 [pmc-release] AID - S0168-1702(14)00405-5 [pii] AID - 10.1016/j.virusres.2014.10.001 [doi] PST - ppublish SO - Virus Res. 2014 Dec 19;194:76-89. doi: 10.1016/j.virusres.2014.10.001. Epub 2014 Oct 13. PMID- 26229568 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20150803 LR - 20200929 IS - 1817-1737 (Print) IS - 1998-3557 (Electronic) IS - 1998-3557 (Linking) VI - 10 IP - 3 DP - 2015 Jul-Sep TI - Re-emerging Middle East respiratory syndrome coronavirus: The hibernating bat hypothesis. PG - 218-9 LID - 10.4103/1817-1737.160847 [doi] FAU - Fakhoury, Hana AU - Fakhoury H AD - Department of Basic Sciences, College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, Riyadh 11481, Saudi Arabia E-mail: hana.fakhoury@gmail.com. FAU - Hajeer, Ali AU - Hajeer A AD - Department of Basic Medical Sciences, College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, Riyadh 14611, Saudi Arabia. LA - eng PT - Journal Article PL - India TA - Ann Thorac Med JT - Annals of thoracic medicine JID - 101280721 PMC - PMC4518356 EDAT- 2015/08/01 06:00 MHDA- 2015/08/01 06:01 PMCR- 2015/07/01 CRDT- 2015/08/01 06:00 PHST- 2015/08/01 06:00 [entrez] PHST- 2015/08/01 06:00 [pubmed] PHST- 2015/08/01 06:01 [medline] PHST- 2015/07/01 00:00 [pmc-release] AID - ATM-10-218 [pii] AID - 10.4103/1817-1737.160847 [doi] PST - ppublish SO - Ann Thorac Med. 2015 Jul-Sep;10(3):218-9. doi: 10.4103/1817-1737.160847. PMID- 29350887 OWN - NLM STAT- MEDLINE DCOM- 20190129 LR - 20210109 IS - 1750-2659 (Electronic) IS - 1750-2640 (Print) IS - 1750-2640 (Linking) VI - 12 IP - 2 DP - 2018 Mar TI - Species-specific clinical characteristics of human coronavirus infection among otherwise healthy adolescents and adults. PG - 299-303 LID - 10.1111/irv.12538 [doi] AB - Human coronavirus (HCoV) is a known cause of influenza-like illness (ILI). In a multisite, observational, longitudinal study of ILI among otherwise healthy adolescents and adults, 12% of subjects were PCR-positive for HCoV. The distribution of species was as follows: HCoV-OC43 (34%), HCoV-229E (28%), HCoV-NL63 (22%), and HCoV-HKU1 (16%). We did not observe species-specific differences in the clinical characteristics of HCoV infection, with the exception of HCoV-HKU1, for which the severity of gastrointestinal symptoms trended higher on the fourth day of illness. CI - © 2018 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. FAU - Bouvier, Monique AU - Bouvier M AD - Betty Irene Moore School of Nursing, University of California Davis, Sacramento, CA, USA. FAU - Chen, Wei-Ju AU - Chen WJ AD - Department of Preventive Medicine and Biostatistics, Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. FAU - Arnold, John C AU - Arnold JC AD - Naval Medical Center, San Diego, CA, USA. FAU - Fairchok, Mary P AU - Fairchok MP AD - Department of Preventive Medicine and Biostatistics, Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. AD - Madigan Army Medical Center, Tacoma, WA, USA. FAU - Danaher, Patrick J AU - Danaher PJ AD - San Antonio Military Medical Center, San Antonio, TX, USA. FAU - Lalani, Tahaniyat AU - Lalani T AD - Department of Preventive Medicine and Biostatistics, Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. FAU - Malone, Leslie AU - Malone L AD - Diatherix Laboratories, LLC, Huntsville, AL, USA. FAU - Mor, Deepika AU - Mor D AD - Department of Preventive Medicine and Biostatistics, Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. FAU - Ridoré, Michelande AU - Ridoré M AD - Children's National Medical Center, Washington, DC, USA. FAU - Burgess, Timothy H AU - Burgess TH AD - Department of Preventive Medicine and Biostatistics, Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. FAU - Millar, Eugene V AU - Millar EV AUID- ORCID: 0000-0002-2019-6480 AD - Department of Preventive Medicine and Biostatistics, Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA. LA - eng GR - Y01 AI005072/AI/NIAID NIH HHS/United States PT - Journal Article PT - Multicenter Study PT - Observational Study PT - Research Support, N.I.H., Extramural DEP - 20180202 PL - England TA - Influenza Other Respir Viruses JT - Influenza and other respiratory viruses JID - 101304007 RN - 0 (RNA, Viral) SB - IM MH - Adolescent MH - Adult MH - Aged MH - Alphacoronavirus/classification/genetics/*isolation & purification/pathogenicity MH - Betacoronavirus/classification/genetics/*isolation & purification/pathogenicity MH - Coronavirus Infections/*pathology/*virology MH - Female MH - Gastrointestinal Diseases/pathology/virology MH - Humans MH - Longitudinal Studies MH - Male MH - Middle Aged MH - Polymerase Chain Reaction MH - RNA, Viral/genetics MH - Respiratory Tract Infections/complications/*pathology/*virology MH - Young Adult PMC - PMC5820427 OTO - NOTNLM OT - clinical characteristics OT - coronavirus OT - influenza-like illness EDAT- 2018/01/20 06:00 MHDA- 2019/01/30 06:00 PMCR- 2018/03/01 CRDT- 2018/01/20 06:00 PHST- 2018/01/03 00:00 [accepted] PHST- 2018/01/20 06:00 [pubmed] PHST- 2019/01/30 06:00 [medline] PHST- 2018/01/20 06:00 [entrez] PHST- 2018/03/01 00:00 [pmc-release] AID - IRV12538 [pii] AID - 10.1111/irv.12538 [doi] PST - ppublish SO - Influenza Other Respir Viruses. 2018 Mar;12(2):299-303. doi: 10.1111/irv.12538. Epub 2018 Feb 2. PMID- 28432925 OWN - NLM STAT- MEDLINE DCOM- 20170717 LR - 20231113 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 507 DP - 2017 Jul TI - Structure of the S1 subunit C-terminal domain from bat-derived coronavirus HKU5 spike protein. PG - 101-109 LID - S0042-6822(17)30124-1 [pii] LID - 10.1016/j.virol.2017.04.016 [doi] AB - Accumulating evidence indicates that MERS-CoV originated from bat coronaviruses (BatCoVs). Previously, we demonstrated that both MERS-CoV and BatCoV HKU4 use CD26 as a receptor, but how the BatCoVs evolved to bind CD26 is an intriguing question. Here, we solved the crystal structure of the S1 subunit C-terminal domain of HKU5 (HKU5-CTD), another BatCoV that is phylogenetically related to MERS-CoV but cannot bind to CD26. We observed that the conserved core subdomain and those of other betacoronaviruses (betaCoVs) have a similar topology of the external subdomain, indicating the same ancestor of lineage C betaCoVs. However, two deletions in two respective loops located in HKU5-CTD result in conformational variations in CD26-binding interface and are responsible for the non-binding of HKU5-CTD to CD26. Combined with sequence variation in the HKU5-CTD receptor binding interface, we propose the necessity for surveilling the mutation in BatCoV HKU5 spike protein in case of bat-to-human interspecies transmission. CI - Copyright © 2017 Elsevier Inc. All rights reserved. FAU - Han, Xue AU - Han X AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Qi, Jianxun AU - Qi J AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Song, Hao AU - Song H AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China. FAU - Wang, Qihui AU - Wang Q AD - CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen 518112, China. FAU - Zhang, Yanfang AU - Zhang Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Wu, Ying AU - Wu Y AD - Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen 518112, China; School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. FAU - Lu, Guangwen AU - Lu G AD - West China Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Pokfulam 999077, Hong Kong Special Administration Region; Department of Microbiology, The University of Hong Kong, Pokfulam 999077, Hong Kong Special Administration Region; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, China. FAU - Shi, Yi AU - Shi Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen 518112, China; Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China. Electronic address: shiyi@im.ac.cn. FAU - Gao, George F AU - Gao GF AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China; Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen 518112, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, China; Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China. Electronic address: gaof@im.ac.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170419 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Amino Acid Sequence MH - Animals MH - Chiroptera/genetics/metabolism/*virology MH - Coronavirus/chemistry/classification/genetics/*metabolism MH - Coronavirus Infections/genetics/metabolism/*veterinary/virology MH - Dipeptidyl Peptidase 4/genetics/metabolism MH - Molecular Sequence Data MH - Protein Conformation MH - Protein Domains MH - Receptors, Virus/genetics/metabolism MH - Sequence Alignment MH - Spike Glycoprotein, Coronavirus/*chemistry/genetics/metabolism PMC - PMC7111649 OTO - NOTNLM OT - BatCoV HKU5 OT - CTD OT - Crystal structure OT - Evolution OT - MERS-CoV EDAT- 2017/04/23 06:00 MHDA- 2017/07/18 06:00 PMCR- 2017/04/19 CRDT- 2017/04/23 06:00 PHST- 2017/03/12 00:00 [received] PHST- 2017/04/10 00:00 [revised] PHST- 2017/04/15 00:00 [accepted] PHST- 2017/04/23 06:00 [pubmed] PHST- 2017/07/18 06:00 [medline] PHST- 2017/04/23 06:00 [entrez] PHST- 2017/04/19 00:00 [pmc-release] AID - S0042-6822(17)30124-1 [pii] AID - 10.1016/j.virol.2017.04.016 [doi] PST - ppublish SO - Virology. 2017 Jul;507:101-109. doi: 10.1016/j.virol.2017.04.016. Epub 2017 Apr 19. PMID- 24960370 OWN - NLM STAT- MEDLINE DCOM- 20150312 LR - 20220311 IS - 1080-6059 (Electronic) IS - 1080-6040 (Print) IS - 1080-6040 (Linking) VI - 20 IP - 7 DP - 2014 Jul TI - Outbreak-related porcine epidemic diarrhea virus strains similar to US strains, South Korea, 2013. PG - 1223-6 LID - 10.3201/eid2007.140294 [doi] AB - In late 2013, outbreaks of porcine epidemic diarrhea virus (PEDV) infection recurred in South Korea. Genetic and phylogenetic analyses showed that isolates from the outbreaks were most closely related to emergent US strains of PEDV. These US strain-like PEDV variants are prevalent in South Korea and responsible for recent outbreaks in the country. FAU - Lee, Sunhee AU - Lee S FAU - Lee, Changhee AU - Lee C LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Emerg Infect Dis JT - Emerging infectious diseases JID - 9508155 SB - IM MH - Animals MH - Coronavirus Infections/*epidemiology/veterinary/virology MH - Disease Outbreaks MH - Phylogeny MH - Porcine epidemic diarrhea virus/*genetics MH - Republic of Korea/epidemiology MH - Swine MH - Swine Diseases/*epidemiology/virology PMC - PMC4073847 OTO - NOTNLM OT - PED OT - PEDV OT - South Korea OT - US strain–like strains OT - economic loss OT - family Coronaviridae OT - genus Alphacoronavirus genus OT - order Nidovirales OT - outbreaks OT - pigs OT - porcine epidemic diarrhea OT - porcine epidemic diarrhea virus OT - pork industry OT - viruses EDAT- 2014/06/25 06:00 MHDA- 2015/03/13 06:00 PMCR- 2014/07/01 CRDT- 2014/06/25 06:00 PHST- 2014/06/25 06:00 [entrez] PHST- 2014/06/25 06:00 [pubmed] PHST- 2015/03/13 06:00 [medline] PHST- 2014/07/01 00:00 [pmc-release] AID - 14-0294 [pii] AID - 10.3201/eid2007.140294 [doi] PST - ppublish SO - Emerg Infect Dis. 2014 Jul;20(7):1223-6. doi: 10.3201/eid2007.140294. PMID- 30530800 OWN - NLM STAT- MEDLINE DCOM- 20190809 LR - 20190809 IS - 2042-7670 (Electronic) IS - 0042-4900 (Linking) VI - 184 IP - 4 DP - 2019 Jan 26 TI - Detection of equine coronavirus in horses in the United Kingdom. PG - 123 LID - 10.1136/vr.105098 [doi] FAU - Bryan, Jill AU - Bryan J AD - Rossdales Laboratories, Rossdale and Partners, Suffolk, UK. FAU - Marr, Celia M AU - Marr CM AD - Rossdales Equine Hospital and Diagnostic Centre, Suffolk, UK. FAU - Mackenzie, Catriona J AU - Mackenzie CJ AD - Rossdales Equine Hospital and Diagnostic Centre, Suffolk, UK. FAU - Mair, Tim S AU - Mair TS AD - Bell Equine Veterinary Clinic, Maidstone, UK. FAU - Fletcher, Adam AU - Fletcher A AD - Rossdales Laboratories, Rossdale and Partners, Suffolk, UK. FAU - Cash, Robert AU - Cash R AD - Rossdales Laboratories, Rossdale and Partners, Suffolk, UK. FAU - Phillips, Monica AU - Phillips M AD - Rossdales Laboratories, Rossdale and Partners, Suffolk, UK. FAU - Pusterla, Nicola AU - Pusterla N AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA. FAU - Mapes, Samantha AU - Mapes S AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA. FAU - Foote, Alastair K AU - Foote AK AD - Rossdales Laboratories, Rossdale and Partners, Suffolk, UK. LA - eng PT - Journal Article DEP - 20181207 PL - England TA - Vet Rec JT - The Veterinary record JID - 0031164 RN - 0 (RNA, Viral) MH - Animals MH - Betacoronavirus 1/*isolation & purification MH - Coronavirus Infections/diagnosis/epidemiology/*veterinary MH - Feces/virology MH - Horse Diseases/*diagnosis/epidemiology/*virology MH - Horses MH - Polymerase Chain Reaction/veterinary MH - RNA, Viral/isolation & purification MH - United Kingdom/epidemiology OTO - NOTNLM OT - PCR OT - enteritis OT - horses OT - viruses COIS- Competing interests: None declared. EDAT- 2018/12/12 06:00 MHDA- 2019/08/10 06:00 CRDT- 2018/12/12 06:00 PHST- 2018/07/05 00:00 [received] PHST- 2018/11/07 00:00 [revised] PHST- 2018/11/16 00:00 [accepted] PHST- 2018/12/12 06:00 [pubmed] PHST- 2019/08/10 06:00 [medline] PHST- 2018/12/12 06:00 [entrez] AID - vr.105098 [pii] AID - 10.1136/vr.105098 [doi] PST - ppublish SO - Vet Rec. 2019 Jan 26;184(4):123. doi: 10.1136/vr.105098. Epub 2018 Dec 7. PMID- 26206723 OWN - NLM STAT- MEDLINE DCOM- 20160426 LR - 20221207 IS - 1878-4380 (Electronic) IS - 0966-842X (Print) IS - 0966-842X (Linking) VI - 23 IP - 8 DP - 2015 Aug TI - Bat-to-human: spike features determining 'host jump' of coronaviruses SARS-CoV, MERS-CoV, and beyond. PG - 468-78 LID - S0966-842X(15)00131-6 [pii] LID - 10.1016/j.tim.2015.06.003 [doi] AB - Both severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) are zoonotic pathogens that crossed the species barriers to infect humans. The mechanism of viral interspecies transmission is an important scientific question to be addressed. These coronaviruses contain a surface-located spike (S) protein that initiates infection by mediating receptor-recognition and membrane fusion and is therefore a key factor in host specificity. In addition, the S protein needs to be cleaved by host proteases before executing fusion, making these proteases a second determinant of coronavirus interspecies infection. Here, we summarize the progress made in the past decade in understanding the cross-species transmission of SARS-CoV and MERS-CoV by focusing on the features of the S protein, its receptor-binding characteristics, and the cleavage process involved in priming. CI - Copyright © 2015 Elsevier Ltd. All rights reserved. FAU - Lu, Guangwen AU - Lu G AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, Sichuan, China. Electronic address: luguangwen2001@126.com. FAU - Wang, Qihui AU - Wang Q AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Gao, George F AU - Gao GF AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Office of Director-General, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China. Electronic address: gaof@im.ac.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20150721 PL - England TA - Trends Microbiol JT - Trends in microbiology JID - 9310916 RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Chiroptera MH - Coronavirus Infections/transmission/*veterinary MH - *Host Specificity MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/genetics/*physiology MH - Protein Binding MH - Severe acute respiratory syndrome-related coronavirus/genetics/*physiology MH - Spike Glycoprotein, Coronavirus/genetics/*metabolism MH - *Viral Tropism MH - Virus Internalization MH - Zoonoses/*transmission PMC - PMC7125587 OTO - NOTNLM OT - MERS-CoV OT - SARS-CoV OT - coronavirus OT - interspecies transmission OT - spike (S) OT - viral and host determinants EDAT- 2015/07/25 06:00 MHDA- 2016/04/27 06:00 PMCR- 2015/07/21 CRDT- 2015/07/25 06:00 PHST- 2015/05/03 00:00 [received] PHST- 2015/06/13 00:00 [revised] PHST- 2015/06/16 00:00 [accepted] PHST- 2015/07/25 06:00 [entrez] PHST- 2015/07/25 06:00 [pubmed] PHST- 2016/04/27 06:00 [medline] PHST- 2015/07/21 00:00 [pmc-release] AID - S0966-842X(15)00131-6 [pii] AID - 10.1016/j.tim.2015.06.003 [doi] PST - ppublish SO - Trends Microbiol. 2015 Aug;23(8):468-78. doi: 10.1016/j.tim.2015.06.003. Epub 2015 Jul 21. PMID- 28190501 OWN - NLM STAT- MEDLINE DCOM- 20170627 LR - 20200407 IS - 1532-2971 (Electronic) IS - 1090-0233 (Print) IS - 1090-0233 (Linking) VI - 220 DP - 2017 Feb TI - Middle East respiratory syndrome (MERS) coronavirus and dromedaries. PG - 75-79 LID - S1090-0233(17)30005-9 [pii] LID - 10.1016/j.tvjl.2016.12.020 [doi] AB - Middle East Respiratory Syndrome (MERS) is a zoonotic viral disease that can be transmitted from dromedaries to human beings. More than 1500 cases of MERS have been reported in human beings to date. Although MERS has been associated with 30% case fatality in human beings, MERS coronavirus (MERS-CoV) infection in dromedaries is usually asymptomatic. In rare cases, dromedaries may develop mild respiratory signs. No MERS-CoV or antibodies against the virus have been detected in camelids other than dromedaries. MERS-CoV is mainly acquired in dromedaries when they are less than 1 year of age, and the proportion of seropositivity increases with age to a seroprevalence of 100% in adult dromedaries. Laboratory diagnosis of MERS-CoV infection in dromedaries can be achieved through virus isolation using Vero cells, RNA detection by real-time quantitative reverse transcriptase-PCR and antigen detection using respiratory specimens or serum. Rapid nucleocapsid antigen detection using a lateral flow platform allows efficient screening of dromedaries carrying MERS-CoV. In addition to MERS-CoV, which is a lineage C virus in the Betacoronavirus (betaCoV) genus, a lineage B betaCoV and a virus in the Alphacoronavirus (alphaCoV) genus have been detected in dromedaries. Dromedary CoV UAE-HKU23 is closely related to human CoV OC43, whereas the alphaCoV has not been detected in human beings to date. CI - Copyright © 2017. Published by Elsevier Ltd. FAU - Wernery, Ulrich AU - Wernery U AD - Central Veterinary Research Laboratory, Dubai, United Arab Emirates. Electronic address: cvrl@cvrl.ae. FAU - Lau, Susanna K P AU - Lau SK AD - Department of Microbiology, University of Hong Kong, Hong Kong, China. FAU - Woo, Patrick C Y AU - Woo PC AD - Department of Microbiology, University of Hong Kong, Hong Kong, China. LA - eng PT - Journal Article PT - Review DEP - 20170109 PL - England TA - Vet J JT - Veterinary journal (London, England : 1997) JID - 9706281 SB - IM CIN - Vet J. 2017 Apr;222:52-53. doi: 10.1016/j.tvjl.2017.02.002. PMID: 28216233 MH - Animals MH - *Camelus MH - Coronavirus Infections/epidemiology/prevention & control/*veterinary/virology MH - *Genome, Viral MH - Middle East Respiratory Syndrome Coronavirus/genetics/*physiology MH - Phylogeny MH - Prevalence MH - Seroepidemiologic Studies MH - Species Specificity MH - Zoonoses/epidemiology/prevention & control/virology PMC - PMC7110516 OTO - NOTNLM OT - Coronavirus OT - Diagnosis OT - Dromedary OT - Middle East respiratory syndrome (MERS) EDAT- 2017/02/14 06:00 MHDA- 2017/06/28 06:00 PMCR- 2017/01/09 CRDT- 2017/02/14 06:00 PHST- 2016/07/06 00:00 [received] PHST- 2016/12/22 00:00 [revised] PHST- 2016/12/28 00:00 [accepted] PHST- 2017/02/14 06:00 [entrez] PHST- 2017/02/14 06:00 [pubmed] PHST- 2017/06/28 06:00 [medline] PHST- 2017/01/09 00:00 [pmc-release] AID - S1090-0233(17)30005-9 [pii] AID - 10.1016/j.tvjl.2016.12.020 [doi] PST - ppublish SO - Vet J. 2017 Feb;220:75-79. doi: 10.1016/j.tvjl.2016.12.020. Epub 2017 Jan 9. PMID- 26063432 OWN - NLM STAT- MEDLINE DCOM- 20160429 LR - 20181113 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 17 DP - 2015 Sep TI - Two Mutations Were Critical for Bat-to-Human Transmission of Middle East Respiratory Syndrome Coronavirus. PG - 9119-23 LID - 10.1128/JVI.01279-15 [doi] AB - To understand how Middle East respiratory syndrome coronavirus (MERS-CoV) transmitted from bats to humans, we compared the virus surface spikes of MERS-CoV and a related bat coronavirus, HKU4. Although HKU4 spike cannot mediate viral entry into human cells, two mutations enabled it to do so by allowing it to be activated by human proteases. These mutations are present in MERS-CoV spike, explaining why MERS-CoV infects human cells. These mutations therefore played critical roles in the bat-to-human transmission of MERS-CoV, either directly or through intermediate hosts. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Yang, Yang AU - Yang Y AD - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA. FAU - Liu, Chang AU - Liu C AD - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA. FAU - Du, Lanying AU - Du L AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA. FAU - Jiang, Shibo AU - Jiang S AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College and Institute of Medical Microbiology, Fudan University, Shanghai, China. FAU - Shi, Zhengli AU - Shi Z AD - Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Baric, Ralph S AU - Baric RS AD - Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina, USA. FAU - Li, Fang AU - Li F AD - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA lifang@umn.edu. LA - eng GR - R01 AI089728/AI/NIAID NIH HHS/United States GR - R01 AI110700/AI/NIAID NIH HHS/United States GR - R01AI089728/AI/NIAID NIH HHS/United States GR - R01AI110700/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20150610 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Receptors, Virus) RN - 0 (Viral Structural Proteins) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Animals MH - Cell Line MH - Chiroptera/virology MH - Coronavirus Infections/genetics/*transmission/virology MH - Dipeptidyl Peptidase 4/*genetics/metabolism MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/genetics/*metabolism MH - Mutation MH - Receptors, Virus/*genetics MH - Viral Structural Proteins/*genetics PMC - PMC4524054 EDAT- 2015/06/13 06:00 MHDA- 2016/04/30 06:00 PMCR- 2015/12/10 CRDT- 2015/06/12 06:00 PHST- 2015/05/15 00:00 [received] PHST- 2015/06/07 00:00 [accepted] PHST- 2015/06/12 06:00 [entrez] PHST- 2015/06/13 06:00 [pubmed] PHST- 2016/04/30 06:00 [medline] PHST- 2015/12/10 00:00 [pmc-release] AID - JVI.01279-15 [pii] AID - 01279-15 [pii] AID - 10.1128/JVI.01279-15 [doi] PST - ppublish SO - J Virol. 2015 Sep;89(17):9119-23. doi: 10.1128/JVI.01279-15. Epub 2015 Jun 10. PMID- 29636467 OWN - NLM STAT- MEDLINE DCOM- 20181115 LR - 20181115 IS - 2222-1751 (Electronic) IS - 2222-1751 (Linking) VI - 7 IP - 1 DP - 2018 Apr 11 TI - Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection. PG - 65 LID - 10.1038/s41426-018-0068-3 [doi] LID - 65 AB - Porcine deltacoronavirus (PDCoV), a member of genus Deltacoronavirus, is an emerging swine enteropathogenic coronavirus (CoV). Although outstanding efforts have led to the identification of Alphacoronavirus and Betacoronavirus receptors, the receptor for Deltacoronavirus is unclear. Here, we compared the amino acid sequences of several representative CoVs. Phylogenetic analysis showed that PDCoV spike (S) protein was close to the cluster containing transmissible gastroenteritis virus (TGEV), which utilizes porcine aminopeptidase N (pAPN) as a functional receptor. Ectopic expression of pAPN in non-susceptible BHK-21 cells rendered them susceptible to PDCoV. These results indicate that pAPN may be a functional receptor for PDCoV infection. However, treatment with APN-specific antibody and inhibitors did not completely block PDCoV infection in IPI-2I porcine intestinal epithelial cells. pAPN knockout in IPI-2I cells completely blocked TGEV infection but only slightly decreased PDCoV infection. Homologous modeling of pAPN with the S1 C-terminal domain (S1-CTD) of PDCoV or TGEV showed that TGEV S1-CTD adopted β-turns (β1-β2 and β3-β4), forming the tip of a β-barrel, to recognize pAPN. However, only the top residues in the β1-β2 turn of PDCoV S1-CTD had the possibility to support an interaction with pAPN, and the β3-β4 turn failed to contact pAPN. We also discuss the evolution and variation of PDCoV S1-CTD based on structure information, providing clues to explain the usage of pAPN by PDCoV. Taken together, the results presented herein reveal that pAPN is likely not a critical functional receptor for PDCoV, although it is involved in PDCoV infection. FAU - Zhu, Xinyu AU - Zhu X AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. FAU - Liu, Shudan AU - Liu S AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. FAU - Wang, Xunlei AU - Wang X AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. FAU - Luo, Zhaochen AU - Luo Z AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. FAU - Shi, Yuejun AU - Shi Y AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. FAU - Wang, Dang AU - Wang D AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. FAU - Peng, Guiqing AU - Peng G AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. FAU - Chen, Huanchun AU - Chen H AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. FAU - Fang, Liurong AU - Fang L AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. fanglr@mail.hzau.edu.cn. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. fanglr@mail.hzau.edu.cn. FAU - Xiao, Shaobo AU - Xiao S AUID- ORCID: 0000-0003-0023-9188 AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. vet@mail.hzau.edu.cn. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. vet@mail.hzau.edu.cn. LA - eng PT - Journal Article DEP - 20180411 PL - United States TA - Emerg Microbes Infect JT - Emerging microbes & infections JID - 101594885 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.11.2 (CD13 Antigens) SB - IM MH - Animals MH - CD13 Antigens/*physiology MH - Cell Line MH - Coronavirus/genetics/*isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - *Host-Pathogen Interactions MH - Phylogeny MH - Receptors, Virus/genetics/*metabolism MH - Spike Glycoprotein, Coronavirus/analysis/genetics MH - Swine MH - Swine Diseases/*virology PMC - PMC5893578 COIS- The authors declare no conflict of interest. EDAT- 2018/04/11 06:00 MHDA- 2018/11/16 06:00 PMCR- 2018/04/11 CRDT- 2018/04/12 06:00 PHST- 2017/12/28 00:00 [received] PHST- 2018/03/21 00:00 [accepted] PHST- 2018/03/18 00:00 [revised] PHST- 2018/04/12 06:00 [entrez] PHST- 2018/04/11 06:00 [pubmed] PHST- 2018/11/16 06:00 [medline] PHST- 2018/04/11 00:00 [pmc-release] AID - 10.1038/s41426-018-0068-3 [pii] AID - 68 [pii] AID - 10.1038/s41426-018-0068-3 [doi] PST - epublish SO - Emerg Microbes Infect. 2018 Apr 11;7(1):65. doi: 10.1038/s41426-018-0068-3. PMID- 25484211 OWN - NLM STAT- MEDLINE DCOM- 20150330 LR - 20190108 IS - 2053-230X (Electronic) IS - 2053-230X (Linking) VI - 70 IP - Pt 12 DP - 2014 Dec 1 TI - Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1. PG - 1620-3 LID - 10.1107/S2053230X14023085 [doi] AB - Human coronavirus HKU1 (HCoV-HKU1), which mainly causes acute self-limited respiratory-tract infections, belongs to group A of the Betacoronavirus genus. Coronavirus genomes encode 16 nonstructural proteins (nsp1-16), which assemble into a large replication-transcription complex mediating virus propagation. Nonstructural protein 9, which binds to the single-stranded DNA/RNA, has been shown to be indispensible for viral replication. Interestingly, a functional mutant (N60K) of nsp9 was identified to compensate for a 6 nt insertion mutation of the 3'-untranslated region (UTR), which is critical for viral RNA synthesis. It has been proposed that the N60K mutation may cause certain conformational changes of nsp9 to rescue the defective insertion mutant. To further investigate the underlying structural mechanism, the N60K mutant of nsp9 from HCoV-HKU1 was successfully crystallized in this study. The crystals diffracted to 2.6 Å resolution and belonged to space group P212121, with unit-cell parameters a = 31.9, b = 85.0, c = 95.0 Å. Two molecules were identified per asymmetric unit. FAU - Chen, Xia AU - Chen X AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Tan, Yusheng AU - Tan Y AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Wang, Fenghua AU - Wang F AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Wang, Jinshan AU - Wang J AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Zhao, Qi AU - Zhao Q AD - Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA. FAU - Li, Shuang AU - Li S AD - Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China. FAU - Fu, Sheng AU - Fu S AD - Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China. FAU - Chen, Cheng AU - Chen C AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Yang, Haitao AU - Yang H AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141114 PL - United States TA - Acta Crystallogr F Struct Biol Commun JT - Acta crystallographica. Section F, Structural biology communications JID - 101620319 RN - 0 (Viral Nonstructural Proteins) SB - IM MH - Amino Acid Sequence MH - Coronavirus/*chemistry/physiology MH - Crystallization MH - Crystallography, X-Ray MH - Molecular Sequence Data MH - Mutation MH - Sequence Homology, Amino Acid MH - Viral Nonstructural Proteins/*chemistry/genetics MH - Virus Replication PMC - PMC4259225 OTO - NOTNLM OT - Human coronavirus HKU1 OT - N60K mutant OT - nonstructural protein 9 EDAT- 2014/12/09 06:00 MHDA- 2015/03/31 06:00 PMCR- 2016/12/01 CRDT- 2014/12/09 06:00 PHST- 2014/08/05 00:00 [received] PHST- 2014/10/20 00:00 [accepted] PHST- 2014/12/09 06:00 [entrez] PHST- 2014/12/09 06:00 [pubmed] PHST- 2015/03/31 06:00 [medline] PHST- 2016/12/01 00:00 [pmc-release] AID - S2053230X14023085 [pii] AID - us5066 [pii] AID - 10.1107/S2053230X14023085 [doi] PST - ppublish SO - Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1620-3. doi: 10.1107/S2053230X14023085. Epub 2014 Nov 14. PMID- 31176401 OWN - NLM STAT- MEDLINE DCOM- 20190626 LR - 20190626 IS - 1873-2542 (Electronic) IS - 0378-1135 (Linking) VI - 233 DP - 2019 Jun TI - ATN-161 reduces virus proliferation in PHEV-infected mice by inhibiting the integrin α5β1-FAK signaling pathway. PG - 147-153 LID - S0378-1135(19)30163-4 [pii] LID - 10.1016/j.vetmic.2019.04.029 [doi] AB - Porcine hemagglutinating encephalomyelitis virus (PHEV) is a typical neurotropic virus that can cause obvious nerve damage. Integrin α5β1 is a transmembrane macromolecular that closely related to neurological function. We recently demonstrated that integrin α5β1 plays a critical role in PHEV invasion in vitro. To determine the function and mechanism of integrin α5β1 in virus proliferation in vivo, we established a mouse model of PHEV infection. Integrin α5β1-FAK signaling pathway was activated in PHEV-infected mice by qPCR, Western blotting, and GST pull-down assays. Viral proliferation and integrin α5β1-FAK signaling pathway were significantly inhibited after intravenous injection of ATN-161, an integrin α5β1 inhibitor. Through a histological analysis, we found that ATN-161-treated mice only showed pathological changes in neuronal cytoplasmic swelling at 5 day post-infection. In summary, our results provide the first evidence that ATN-161 inhibits the proliferation of PHEV in mice and explores its underlying mechanisms of action. CI - Copyright © 2019 Elsevier B.V. All rights reserved. FAU - Lv, Xiaoling AU - Lv X AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. FAU - Li, Zi AU - Li Z AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. FAU - Guan, Jiyu AU - Guan J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. FAU - Zhang, Jing AU - Zhang J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. FAU - Xu, Baofeng AU - Xu B AD - Department of Neurosurgery, The First Hospital of Jilin University, Changchun, 130021, China. FAU - He, Wenqi AU - He W AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. FAU - Lan, Yungang AU - Lan Y AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. FAU - Zhao, Kui AU - Zhao K AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. FAU - Lu, Huijun AU - Lu H AD - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China. FAU - Song, Deguang AU - Song D AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. FAU - Gao, Feng AU - Gao F AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. Electronic address: gaofeng@jlu.edu.cn. LA - eng PT - Journal Article DEP - 20190426 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Antiviral Agents) RN - 0 (Integrin alpha5beta1) RN - 0 (Peptides) SB - IM MH - Animals MH - Antiviral Agents/*administration & dosage MH - Betacoronavirus 1/genetics/*physiology MH - Disease Models, Animal MH - Integrin alpha5beta1/*antagonists & inhibitors/metabolism MH - Mice MH - Mice, Inbred BALB C MH - Peptides/*administration & dosage MH - Real-Time Polymerase Chain Reaction MH - Signal Transduction MH - *Virus Replication OTO - NOTNLM OT - ATN-161 OT - FAK OT - Integrin α5β1 OT - Porcine hemagglutinating encephalomyelitis virus EDAT- 2019/06/10 06:00 MHDA- 2019/06/27 06:00 CRDT- 2019/06/10 06:00 PHST- 2019/02/01 00:00 [received] PHST- 2019/04/24 00:00 [revised] PHST- 2019/04/24 00:00 [accepted] PHST- 2019/06/10 06:00 [entrez] PHST- 2019/06/10 06:00 [pubmed] PHST- 2019/06/27 06:00 [medline] AID - S0378-1135(19)30163-4 [pii] AID - 10.1016/j.vetmic.2019.04.029 [doi] PST - ppublish SO - Vet Microbiol. 2019 Jun;233:147-153. doi: 10.1016/j.vetmic.2019.04.029. Epub 2019 Apr 26. PMID- 26695637 OWN - NLM STAT- MEDLINE DCOM- 20161006 LR - 20231111 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 12 DP - 2015 Dec 22 TI - MERS coronavirus: diagnostics, epidemiology and transmission. PG - 222 LID - 10.1186/s12985-015-0439-5 [doi] LID - 222 AB - The first known cases of Middle East respiratory syndrome (MERS), associated with infection by a novel coronavirus (CoV), occurred in 2012 in Jordan but were reported retrospectively. The case first to be publicly reported was from Jeddah, in the Kingdom of Saudi Arabia (KSA). Since then, MERS-CoV sequences have been found in a bat and in many dromedary camels (DC). MERS-CoV is enzootic in DC across the Arabian Peninsula and in parts of Africa, causing mild upper respiratory tract illness in its camel reservoir and sporadic, but relatively rare human infections. Precisely how virus transmits to humans remains unknown but close and lengthy exposure appears to be a requirement. The KSA is the focal point of MERS, with the majority of human cases. In humans, MERS is mostly known as a lower respiratory tract (LRT) disease involving fever, cough, breathing difficulties and pneumonia that may progress to acute respiratory distress syndrome, multiorgan failure and death in 20% to 40% of those infected. However, MERS-CoV has also been detected in mild and influenza-like illnesses and in those with no signs or symptoms. Older males most obviously suffer severe disease and MERS patients often have comorbidities. Compared to severe acute respiratory syndrome (SARS), another sometimes- fatal zoonotic coronavirus disease that has since disappeared, MERS progresses more rapidly to respiratory failure and acute kidney injury (it also has an affinity for growth in kidney cells under laboratory conditions), is more frequently reported in patients with underlying disease and is more often fatal. Most human cases of MERS have been linked to lapses in infection prevention and control (IPC) in healthcare settings, with approximately 20% of all virus detections reported among healthcare workers (HCWs) and higher exposures in those with occupations that bring them into close contact with camels. Sero-surveys have found widespread evidence of past infection in adult camels and limited past exposure among humans. Sensitive, validated reverse transcriptase real-time polymerase chain reaction (RT-rtPCR)-based diagnostics have been available almost from the start of the emergence of MERS. While the basic virology of MERS-CoV has advanced over the past three years, understanding of the interplay between camel, environment, and human remains limited. FAU - Mackay, Ian M AU - Mackay IM AD - Department of Health, Public and Environmental Health Virology Laboratory, Forensic and Scientific Services, Archerfield, QLD, Australia. ian.mackay.im@gmail.com. AD - The University of Queensland, St Lucia, QLD, Australia. ian.mackay.im@gmail.com. AD - Queensland University of Technology, George St, Brisbane, QLD, Australia. ian.mackay.im@gmail.com. FAU - Arden, Katherine E AU - Arden KE AD - The University of Queensland, St Lucia, QLD, Australia. LA - eng PT - Journal Article PT - Review DEP - 20151222 PL - England TA - Virol J JT - Virology journal JID - 101231645 SB - IM MH - Animals MH - Camelus MH - Coronavirus Infections/diagnosis/*epidemiology/transmission/*veterinary MH - Cross Infection/*transmission MH - *Disease Transmission, Infectious MH - Global Health MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*isolation & purification MH - Molecular Diagnostic Techniques/methods MH - Occupational Exposure MH - Real-Time Polymerase Chain Reaction/methods MH - Reverse Transcriptase Polymerase Chain Reaction/methods MH - Zoonoses/*transmission PMC - PMC4687373 EDAT- 2015/12/24 06:00 MHDA- 2016/10/08 06:00 PMCR- 2015/12/22 CRDT- 2015/12/24 06:00 PHST- 2015/09/14 00:00 [received] PHST- 2015/11/27 00:00 [accepted] PHST- 2015/12/24 06:00 [entrez] PHST- 2015/12/24 06:00 [pubmed] PHST- 2016/10/08 06:00 [medline] PHST- 2015/12/22 00:00 [pmc-release] AID - 10.1186/s12985-015-0439-5 [pii] AID - 439 [pii] AID - 10.1186/s12985-015-0439-5 [doi] PST - epublish SO - Virol J. 2015 Dec 22;12:222. doi: 10.1186/s12985-015-0439-5. PMID- 31319854 OWN - NLM STAT- MEDLINE DCOM- 20191203 LR - 20231013 IS - 1746-6148 (Electronic) IS - 1746-6148 (Linking) VI - 15 IP - 1 DP - 2019 Jul 18 TI - The emergence of porcine epidemic diarrhoea in Croatia: molecular characterization and serology. PG - 249 LID - 10.1186/s12917-019-2002-x [doi] LID - 249 AB - BACKGROUND: Porcine epidemic diarrhoea (PED) is an emergent/re-emergent viral pig disease (caused by the virus belonging to the Coronaviridae family, in specific the Alphacoronavirus genus) of global importance. Clinical presentation is characterized with acute diarrhoea, vomiting and dehydration in pigs of all ages, with a possible high mortality in suckling piglets. The disease emerged in the USA in 2013 causing heavy losses, and re-emerged in Europe in 2014, but with milder consequences. RESULTS: In the spring 2016, PED-like symptoms were reported to be seen on an agricultural holding in Eastern Croatia; laboratory workup confirmed the Croatia's first PED outbreak ever. Porcine epidemic diarrhoea virus (PEDV) strain responsible for the outbreak was of the S-INDEL genotype, much the same as other European PEDV strains. In 2017, a post-outbreak serology was carried out in three counties in Eastern Croatia, revealing seropositivity in pigs bred on four large industrial holdings (9.09%). The seroprevalence across PEDV-positive holdings was up to 82.8%. The latter holdings were unanimously managed by an enterprise that had never reported PED before. CONCLUSIONS: PED has emerged in Croatian pig population causing potentially considerable losses. The circulating strain was of the S-INDEL genotype. Serological workup proved PEDV spread to another four agricultural holdings, demonstrating the importance of not only external, but also internal biosecurity measures. FAU - Brnić, Dragan AU - Brnić D AUID- ORCID: 0000-0002-7318-8337 AD - Virology Department, Croatian Veterinary Institute, Savska Cesta 143, 10000, Zagreb, Croatia. brnic@veinst.hr. FAU - Šimić, Ivana AU - Šimić I AD - Virology Department, Croatian Veterinary Institute, Savska Cesta 143, 10000, Zagreb, Croatia. FAU - Lojkić, Ivana AU - Lojkić I AD - Virology Department, Croatian Veterinary Institute, Savska Cesta 143, 10000, Zagreb, Croatia. FAU - Krešić, Nina AU - Krešić N AD - Virology Department, Croatian Veterinary Institute, Savska Cesta 143, 10000, Zagreb, Croatia. FAU - Jungić, Andreja AU - Jungić A AD - Virology Department, Croatian Veterinary Institute, Savska Cesta 143, 10000, Zagreb, Croatia. FAU - Balić, Davor AU - Balić D AD - Veterinary Department Vinkovci, Croatian Veterinary Institute, Josipa Kozarca 24, 32100, Vinkovci, Croatia. FAU - Lolić, Marica AU - Lolić M AD - Veterinary Department Vinkovci, Croatian Veterinary Institute, Josipa Kozarca 24, 32100, Vinkovci, Croatia. FAU - Knežević, Dražen AU - Knežević D AD - Croatian Agency for Agriculture and Food, Ivana Gundulića 36b, 31000, Osijek, Croatia. FAU - Hengl, Brigita AU - Hengl B AD - Croatian Agency for Agriculture and Food, Ivana Gundulića 36b, 31000, Osijek, Croatia. LA - eng GR - 6/17/Croatian Food Agency/ PT - Journal Article DEP - 20190718 PL - England TA - BMC Vet Res JT - BMC veterinary research JID - 101249759 SB - IM MH - Animals MH - Coronavirus Infections/epidemiology/*veterinary MH - Croatia/epidemiology MH - Disease Outbreaks/veterinary MH - Molecular Epidemiology MH - Porcine epidemic diarrhea virus/genetics/*isolation & purification MH - Seroepidemiologic Studies MH - Swine MH - Swine Diseases/*epidemiology/virology PMC - PMC6637520 OTO - NOTNLM OT - Croatia OT - Domestic pig OT - ELISA OT - Outbreak OT - Porcine epidemic diarrhoea virus OT - S-INDEL COIS- The authors declare that they have no competing interests. EDAT- 2019/07/20 06:00 MHDA- 2019/12/04 06:00 PMCR- 2019/07/18 CRDT- 2019/07/20 06:00 PHST- 2019/02/01 00:00 [received] PHST- 2019/07/11 00:00 [accepted] PHST- 2019/07/20 06:00 [entrez] PHST- 2019/07/20 06:00 [pubmed] PHST- 2019/12/04 06:00 [medline] PHST- 2019/07/18 00:00 [pmc-release] AID - 10.1186/s12917-019-2002-x [pii] AID - 2002 [pii] AID - 10.1186/s12917-019-2002-x [doi] PST - epublish SO - BMC Vet Res. 2019 Jul 18;15(1):249. doi: 10.1186/s12917-019-2002-x. PMID- 26584230 OWN - NLM STAT- MEDLINE DCOM- 20160725 LR - 20181113 IS - 1080-6059 (Electronic) IS - 1080-6040 (Print) IS - 1080-6040 (Linking) VI - 21 IP - 12 DP - 2015 Dec TI - Isolation of Porcine Epidemic Diarrhea Virus during Outbreaks in South Korea, 2013-2014. PG - 2238-40 LID - 10.3201/eid2112.150437 [doi] FAU - Chung, Hee-Chun AU - Chung HC FAU - Nguyen, Van Giap AU - Nguyen VG FAU - Moon, Hyoung-Joon AU - Moon HJ FAU - Lee, Jee-Hoon AU - Lee JH FAU - Park, Seong-Jun AU - Park SJ FAU - Lee, Ga-Eun AU - Lee GE FAU - Kim, Hye-Kwon AU - Kim HK FAU - Noh, You-Shun AU - Noh YS FAU - Lee, Chan-Hee AU - Lee CH FAU - Goede, Dane AU - Goede D FAU - Park, Bong Kyun AU - Park BK LA - eng PT - Letter PT - Research Support, Non-U.S. Gov't PL - United States TA - Emerg Infect Dis JT - Emerging infectious diseases JID - 9508155 SB - IM MH - Animals MH - Coronavirus Infections/*epidemiology/virology MH - Disease Outbreaks/prevention & control MH - Patient Isolation/*methods MH - Porcine epidemic diarrhea virus/genetics/isolation & purification/*pathogenicity MH - Republic of Korea/epidemiology MH - Swine/*genetics/virology MH - Swine Diseases/epidemiology/pathology/virology PMC - PMC4672425 OTO - NOTNLM OT - Alphacoronavirus OT - Coronaviridae OT - Nidovirales OT - North American strain–like strains OT - PED OT - PEDV OT - South Korea OT - isolation OT - outbreaks OT - pigs OT - porcine epidemic diarrhea OT - porcine epidemic diarrhea virus OT - viruses EDAT- 2015/11/20 06:00 MHDA- 2016/07/28 06:00 PMCR- 2015/12/01 CRDT- 2015/11/20 06:00 PHST- 2015/11/20 06:00 [entrez] PHST- 2015/11/20 06:00 [pubmed] PHST- 2016/07/28 06:00 [medline] PHST- 2015/12/01 00:00 [pmc-release] AID - 15-0437 [pii] AID - 10.3201/eid2112.150437 [doi] PST - ppublish SO - Emerg Infect Dis. 2015 Dec;21(12):2238-40. doi: 10.3201/eid2112.150437. PMID- 24766432 OWN - NLM STAT- MEDLINE DCOM- 20150105 LR - 20240328 IS - 1744-8395 (Electronic) IS - 1476-0584 (Print) IS - 1476-0584 (Linking) VI - 13 IP - 6 DP - 2014 Jun TI - Current advancements and potential strategies in the development of MERS-CoV vaccines. PG - 761-74 LID - 10.1586/14760584.2014.912134 [doi] AB - Middle East respiratory syndrome (MERS) is a newly emerging infectious disease caused by a novel coronavirus, MERS-coronavirus (MERS-CoV), a new member in the lineage C of β-coronavirus (β-CoV). The increased human cases and high mortality rate of MERS-CoV infection make it essential to develop safe and effective vaccines. In this review, the current advancements and potential strategies in the development of MERS vaccines, particularly subunit vaccines based on MERS-CoV spike (S) protein and its receptor-binding domain (RBD), are discussed. How to improve the efficacy of subunit vaccines through novel adjuvant formulations and routes of administration as well as currently available animal models for evaluating the in vivo efficacy of MERS-CoV vaccines are also addressed. Overall, these strategies may have important implications for the development of effective and safe vaccines for MERS-CoV in the future. FAU - Zhang, Naru AU - Zhang N AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY, USA. FAU - Jiang, Shibo AU - Jiang S FAU - Du, Lanying AU - Du L LA - eng GR - R21 AI109094/AI/NIAID NIH HHS/United States GR - AI109094/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Review DEP - 20140426 PL - England TA - Expert Rev Vaccines JT - Expert review of vaccines JID - 101155475 RN - 0 (Adjuvants, Immunologic) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Vaccines, Subunit) RN - 0 (Viral Vaccines) SB - IM MH - Adjuvants, Immunologic/administration & dosage/isolation & purification MH - Animals MH - Coronavirus Infections/*prevention & control MH - Disease Models, Animal MH - Drug Discovery/trends MH - Humans MH - Middle East/epidemiology MH - Middle East Respiratory Syndrome Coronavirus/genetics/*immunology MH - Spike Glycoprotein, Coronavirus/genetics/immunology MH - Vaccination/methods MH - Vaccines, Subunit/genetics/immunology/isolation & purification MH - Viral Vaccines/genetics/*immunology/*isolation & purification PMC - PMC4241375 MID - NIHMS616868 OTO - NOTNLM OT - MERS-CoV OT - Middle East respiratory syndrome OT - adjuvants OT - administration routes OT - coronavirus OT - receptor-binding domain OT - spike protein OT - subunit vaccines OT - vaccines EDAT- 2014/04/29 06:00 MHDA- 2015/01/06 06:00 PMCR- 2020/03/30 CRDT- 2014/04/29 06:00 PHST- 2014/04/29 06:00 [entrez] PHST- 2014/04/29 06:00 [pubmed] PHST- 2015/01/06 06:00 [medline] PHST- 2020/03/30 00:00 [pmc-release] AID - 912134 [pii] AID - 10.1586/14760584.2014.912134 [doi] PST - ppublish SO - Expert Rev Vaccines. 2014 Jun;13(6):761-74. doi: 10.1586/14760584.2014.912134. Epub 2014 Apr 26. PMID- 30310104 OWN - NLM STAT- MEDLINE DCOM- 20191029 LR - 20230928 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 8 IP - 1 DP - 2018 Oct 11 TI - Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission. PG - 15177 LID - 10.1038/s41598-018-33487-8 [doi] LID - 15177 AB - A 29 nucleotide deletion in open reading frame 8 (ORF8) is the most obvious genetic change in severe acute respiratory syndrome coronavirus (SARS-CoV) during its emergence in humans. In spite of intense study, it remains unclear whether the deletion actually reflects adaptation to humans. Here we engineered full, partially deleted (-29 nt), and fully deleted ORF8 into a SARS-CoV infectious cDNA clone, strain Frankfurt-1. Replication of the resulting viruses was compared in primate cell cultures as well as Rhinolophus bat cells made permissive for SARS-CoV replication by lentiviral transduction of the human angiotensin-converting enzyme 2 receptor. Cells from cotton rat, goat, and sheep provided control scenarios that represent host systems in which SARS-CoV is neither endemic nor epidemic. Independent of the cell system, the truncation of ORF8 (29 nt deletion) decreased replication up to 23-fold. The effect was independent of the type I interferon response. The 29 nt deletion in SARS-CoV is a deleterious mutation acquired along the initial human-to-human transmission chain. The resulting loss of fitness may be due to a founder effect, which has rarely been documented in processes of viral emergence. These results have important implications for the retrospective assessment of the threat posed by SARS. FAU - Muth, Doreen AU - Muth D AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117, Berlin, Germany. AD - German Center for Infection Research (DZIF), Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. FAU - Corman, Victor Max AU - Corman VM AUID- ORCID: 0000-0002-3605-0136 AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117, Berlin, Germany. AD - German Center for Infection Research (DZIF), Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. FAU - Roth, Hanna AU - Roth H AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. FAU - Binger, Tabea AU - Binger T AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. FAU - Dijkman, Ronald AU - Dijkman R AUID- ORCID: 0000-0003-0320-2743 AD - Federal Department of Home Affairs, Institute of Virology and Immunology IVI, Bern and Mittelhäusern, Sensemattstrasse 293, 3147, Mittelhäusern, Switzerland. AD - Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, 3012, Bern, Switzerland. FAU - Gottula, Lina Theresa AU - Gottula LT AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117, Berlin, Germany. AD - German Center for Infection Research (DZIF), Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. FAU - Gloza-Rausch, Florian AU - Gloza-Rausch F AD - Noctalis, Centre for Bat Protection and Information, Oberbergstraße 27, 23795, Bad Segeberg, Germany. FAU - Balboni, Andrea AU - Balboni A AUID- ORCID: 0000-0002-8049-6645 AD - Dipartimento di Scienze Mediche Veterinarie, Facoltà di Medicina Veterinaria, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064, Ozzano Emilia, (BO), Italy. FAU - Battilani, Mara AU - Battilani M AD - Dipartimento di Scienze Mediche Veterinarie, Facoltà di Medicina Veterinaria, Alma Mater Studiorum-Università di Bologna, Via Tolara di Sopra 50, 40064, Ozzano Emilia, (BO), Italy. FAU - Rihtarič, Danijela AU - Rihtarič D AD - Virology Unit, Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia. FAU - Toplak, Ivan AU - Toplak I AD - Virology Unit, Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia. FAU - Ameneiros, Ramón Seage AU - Ameneiros RS AD - Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Albert-Einstein Allee 11, 89069, Ulm, Germany. AD - Group Morcegos de Galicia, Drosera Society, Pdo. Magdalena, G-2, 2° esq, 15320, As Pontes, Spain. FAU - Pfeifer, Alexander AU - Pfeifer A AD - Institute for Pharmacology and Toxicology, University of Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. FAU - Thiel, Volker AU - Thiel V AD - Federal Department of Home Affairs, Institute of Virology and Immunology IVI, Bern and Mittelhäusern, Sensemattstrasse 293, 3147, Mittelhäusern, Switzerland. AD - Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, 3012, Bern, Switzerland. FAU - Drexler, Jan Felix AU - Drexler JF AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117, Berlin, Germany. AD - German Center for Infection Research (DZIF), Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. FAU - Müller, Marcel Alexander AU - Müller MA AUID- ORCID: 0000-0003-2242-5117 AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117, Berlin, Germany. AD - German Center for Infection Research (DZIF), Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. FAU - Drosten, Christian AU - Drosten C AD - Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, Charitéplatz 1, 10117, Berlin, Germany. christian.drosten@charite.de. AD - German Center for Infection Research (DZIF), Berlin, Germany. christian.drosten@charite.de. AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. christian.drosten@charite.de. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181011 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (RNA, Viral) RN - 0 (Recombinant Proteins) RN - 0 (Viral Matrix Proteins) RN - 0 (sars7a protein, SARS virus) SB - IM CIN - Nature. 2020 Feb;578(7793):15-16. doi: 10.1038/d41586-020-00236-9. PMID: 32020111 MH - Animals MH - Cell Line MH - Cells, Cultured MH - Chiroptera/virology MH - Disease Reservoirs MH - *Host-Pathogen Interactions MH - Humans MH - *RNA, Viral MH - Recombinant Proteins MH - Severe acute respiratory syndrome-related coronavirus/*physiology MH - *Sequence Deletion MH - Severe Acute Respiratory Syndrome/*transmission/*virology MH - Viral Matrix Proteins/genetics/metabolism MH - Virus Replication/*genetics PMC - PMC6181990 COIS- The authors declare no competing interests. EDAT- 2018/10/13 06:00 MHDA- 2019/10/30 06:00 PMCR- 2018/10/11 CRDT- 2018/10/13 06:00 PHST- 2018/07/16 00:00 [received] PHST- 2018/09/27 00:00 [accepted] PHST- 2018/10/13 06:00 [entrez] PHST- 2018/10/13 06:00 [pubmed] PHST- 2019/10/30 06:00 [medline] PHST- 2018/10/11 00:00 [pmc-release] AID - 10.1038/s41598-018-33487-8 [pii] AID - 33487 [pii] AID - 10.1038/s41598-018-33487-8 [doi] PST - epublish SO - Sci Rep. 2018 Oct 11;8(1):15177. doi: 10.1038/s41598-018-33487-8. PMID- 31455974 OWN - NLM STAT- MEDLINE DCOM- 20191105 LR - 20200324 IS - 1432-1289 (Electronic) IS - 0020-9554 (Print) IS - 0020-9554 (Linking) VI - 60 IP - 11 DP - 2019 Nov TI - [Coronaviruses as the cause of respiratory infections]. PG - 1136-1145 LID - 10.1007/s00108-019-00671-5 [doi] AB - BACKGROUND: There are six human pathogenic coronaviruses (CoV), which mainly cause infections of the respiratory system. In everyday clinical practice, it is helpful to know the relevance and characteristics of these pathogens. OBJECTIVE: To present the epidemiology, clinical picture and differences of human pathogenic CoV and to provide information on the diagnostics and treatment of patients suspected of having CoV infections. MATERIAL AND METHODS: Selective literature search, presentation of results and discussion of fundamental works and expert recommendations, including publications by the World Health Organization (WHO), the European Centre for Disease Prevention and Control (ECDC) and the Robert Koch Institute. RESULTS: The four endemic human CoVs (HCoV-NL63, HCoV-229E, HCoV-OC43 and HCoV-HKU1) mainly cause mild respiratory tract infections. In addition to these four endemic HCoV, the two epidemic CoV, severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV can cause severe pneumonia. The SARS-CoV has not been detected in humans in the last 15 years and MERS-CoV has been circulating mainly on the Arabian Peninsula since 2012; however, neither a specific treatment nor approved vaccines exist for any of the six human pathogenic CoVs. CONCLUSION: All six human CoVs can be diagnosed using RT-PCR on respiratory specimens but this is rarely necessary for the four endemic strains. In current clinical practice SARS-CoV has no importance as it has not been detected in humans for 15 years; however, a possible MERS-CoV infection should be taken into account in patients with typical symptoms and travel history to endemic regions. In this case, rapid diagnostic and general hygiene practices are important to prevent further transmission. FAU - Corman, V M AU - Corman VM AD - Institut für Virologie, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Deutschland. FAU - Lienau, J AU - Lienau J AD - Arbeitsbereich Pulmonale Inflammation, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Deutschland. FAU - Witzenrath, M AU - Witzenrath M AD - Arbeitsbereich Pulmonale Inflammation, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Deutschland. martin.witzenrath@charite.de. AD - Medizinische Klinik mit Schwerpunkt Infektiologie und Pneumologie, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Deutschland. martin.witzenrath@charite.de. LA - ger PT - Journal Article PT - Review TT - Coronaviren als Ursache respiratorischer Infektionen. PL - Germany TA - Internist (Berl) JT - Der Internist JID - 0264620 SB - IM MH - Betacoronavirus MH - *Coronavirus MH - Coronavirus 229E, Human MH - Coronavirus Infections/*diagnosis/epidemiology/*prevention & control/virology MH - Coronavirus NL63, Human MH - Coronavirus OC43, Human MH - Humans MH - *Respiratory Tract Infections/diagnosis/epidemiology/virology MH - Severe Acute Respiratory Syndrome/*diagnosis/epidemiology/prevention & control/virology PMC - PMC7079972 OTO - NOTNLM OT - Common cold OT - Middle East respiratory syndrome coronavirus (MERS-CoV) OT - Pneumonia OT - Respiratory tract infections OT - Severe acute respiratory syndrome (SARS) EDAT- 2019/08/29 06:00 MHDA- 2019/11/07 06:00 PMCR- 2020/03/18 CRDT- 2019/08/29 06:00 PHST- 2019/08/29 06:00 [pubmed] PHST- 2019/11/07 06:00 [medline] PHST- 2019/08/29 06:00 [entrez] PHST- 2020/03/18 00:00 [pmc-release] AID - 10.1007/s00108-019-00671-5 [pii] AID - 671 [pii] AID - 10.1007/s00108-019-00671-5 [doi] PST - ppublish SO - Internist (Berl). 2019 Nov;60(11):1136-1145. doi: 10.1007/s00108-019-00671-5. PMID- 31533860 OWN - NLM STAT- MEDLINE DCOM- 20191216 LR - 20200225 IS - 1297-9716 (Electronic) IS - 0928-4249 (Print) IS - 0928-4249 (Linking) VI - 50 IP - 1 DP - 2019 Sep 18 TI - Assessing exhibition swine as potential disseminators of infectious disease through the detection of five respiratory pathogens at agricultural exhibitions. PG - 63 LID - 10.1186/s13567-019-0684-5 [doi] LID - 63 AB - Widespread geographic movement and extensive comingling of exhibition swine facilitates the spread and transmission of infectious pathogens. Nasal samples were collected from 2862 pigs at 102 exhibitions and tested for five pathogens. At least one pathogen was molecularly detected in pigs at 63 (61.8%) exhibitions. Influenza A virus was most prevalent and was detected in 498 (17.4%) samples. Influenza D virus was detected in two (0.07%) samples. More than one pathogen was detected in 165 (5.8%) samples. Influenza A virus remains a top threat to animal and human health, but other pathogens may be disseminated through the exhibition swine population. FAU - Lauterbach, Sarah E AU - Lauterbach SE AD - Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Road, Columbus, OH, 43210, USA. FAU - Nelson, Sarah W AU - Nelson SW AD - Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Road, Columbus, OH, 43210, USA. FAU - Robinson, Meghann E AU - Robinson ME AD - Health Science District, University of California Davis, 1 Garrod Drive, Davis, CA, 95616, USA. FAU - Lorbach, Josh N AU - Lorbach JN AD - Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Road, Columbus, OH, 43210, USA. FAU - Nolting, Jacqueline M AU - Nolting JM AD - Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Road, Columbus, OH, 43210, USA. FAU - Bowman, Andrew S AU - Bowman AS AUID- ORCID: 0000-0002-0738-8453 AD - Department of Veterinary Preventive Medicine, The Ohio State University, 1920 Coffey Road, Columbus, OH, 43210, USA. bowman.214@osu.edu. LA - eng GR - HHSN272201400006C/AI/NIAID NIH HHS/United States GR - HHSN266200700007C/National Institute of Allergy and Infectious Diseases/ PT - Journal Article DEP - 20190918 PL - England TA - Vet Res JT - Veterinary research JID - 9309551 SB - IM MH - Animals MH - Betacoronavirus 1/isolation & purification MH - Coronavirus Infections/epidemiology/veterinary/virology MH - Influenza A virus/isolation & purification MH - Orthomyxoviridae Infections/epidemiology/veterinary/virology MH - Porcine Reproductive and Respiratory Syndrome/epidemiology/virology MH - Porcine respiratory and reproductive syndrome virus/isolation & purification MH - Prevalence MH - Respiratory Tract Diseases/epidemiology/*veterinary/virology MH - Respirovirus/isolation & purification MH - Respirovirus Infections/epidemiology/veterinary/virology MH - Sus scrofa MH - Swine MH - Swine Diseases/*epidemiology/virology MH - Thogotovirus/isolation & purification MH - United States/epidemiology PMC - PMC6749708 COIS- The authors declare that they have no competing interests. EDAT- 2019/09/20 06:00 MHDA- 2019/12/18 06:00 PMCR- 2019/09/18 CRDT- 2019/09/20 06:00 PHST- 2019/07/12 00:00 [received] PHST- 2019/08/27 00:00 [accepted] PHST- 2019/09/20 06:00 [entrez] PHST- 2019/09/20 06:00 [pubmed] PHST- 2019/12/18 06:00 [medline] PHST- 2019/09/18 00:00 [pmc-release] AID - 10.1186/s13567-019-0684-5 [pii] AID - 684 [pii] AID - 10.1186/s13567-019-0684-5 [doi] PST - epublish SO - Vet Res. 2019 Sep 18;50(1):63. doi: 10.1186/s13567-019-0684-5. PMID- 25462342 OWN - NLM STAT- MEDLINE DCOM- 20150306 LR - 20201209 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 475 DP - 2015 Jan 15 TI - SHAPE analysis of the RNA secondary structure of the Mouse Hepatitis Virus 5' untranslated region and N-terminal nsp1 coding sequences. PG - 15-27 LID - S0042-6822(14)00495-4 [pii] LID - 10.1016/j.virol.2014.11.001 [doi] AB - SHAPE technology was used to analyze RNA secondary structure of the 5' most 474 nts of the MHV-A59 genome encompassing the minimal 5' cis-acting region required for defective interfering RNA replication. The structures generated were in agreement with previous characterizations of SL1 through SL4 and two recently predicted secondary structure elements, S5 and SL5A. SHAPE provided biochemical support for four additional stem-loops not previously functionally investigated in MHV. Secondary structure predictions for 5' regions of MHV-A59, BCoV and SARS-CoV were similar despite high sequence divergence. The pattern of SHAPE reactivity of in virio genomic RNA, ex virio genomic RNA, and in vitro synthesized RNA was similar, suggesting that binding of N protein or other proteins to virion RNA fails to protect the RNA from reaction with lipid permeable SHAPE reagent. Reverse genetic experiments suggested that SL5C and SL6 within the nsp1 coding sequence are not required for viral replication. CI - Copyright © 2014 Elsevier Inc. All rights reserved. FAU - Yang, Dong AU - Yang D AD - Department of Microbial Pathogenesis and Immunology, Texas A&M University, College of Medicine, 407 Reynolds Medical Building, 1114 TAMU, College Station, TX 77843-1114, USA. FAU - Liu, Pinghua AU - Liu P AD - Department of Microbial Pathogenesis and Immunology, Texas A&M University, College of Medicine, 407 Reynolds Medical Building, 1114 TAMU, College Station, TX 77843-1114, USA. FAU - Wudeck, Elyse V AU - Wudeck EV AD - Department of Microbial Pathogenesis and Immunology, Texas A&M University, College of Medicine, 407 Reynolds Medical Building, 1114 TAMU, College Station, TX 77843-1114, USA. FAU - Giedroc, David P AU - Giedroc DP AD - Department of Chemistry, Indiana University, Bloomington, IN 47405-7102, USA. FAU - Leibowitz, Julian L AU - Leibowitz JL AD - Department of Microbial Pathogenesis and Immunology, Texas A&M University, College of Medicine, 407 Reynolds Medical Building, 1114 TAMU, College Station, TX 77843-1114, USA. Electronic address: jleibowitz@tamu.edu. LA - eng GR - R01 AI051493/AI/NIAID NIH HHS/United States GR - R01 AI067416/AI/NIAID NIH HHS/United States GR - AI067416/AI/NIAID NIH HHS/United States GR - AI051493/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20141121 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (5' Untranslated Regions) RN - 0 (NSP1 protein, murine hepatitis virus) RN - 0 (RNA, Viral) RN - 0 (Viral Nonstructural Proteins) SB - IM MH - 5' Untranslated Regions/*genetics MH - Animals MH - Gene Expression Regulation, Viral/*physiology MH - Mice MH - Murine hepatitis virus/genetics/*metabolism MH - Nucleic Acid Conformation MH - RNA, Viral/*chemistry/metabolism MH - Viral Nonstructural Proteins/genetics/*metabolism PMC - PMC4280293 MID - NIHMS641531 OTO - NOTNLM OT - 5′ cis-acting region OT - Betacoronavirus OT - Mouse Hepatitis Virus OT - Murine coronavirus OT - RNA secondary structure OT - SHAPE analysis EDAT- 2014/12/03 06:00 MHDA- 2015/03/07 06:00 PMCR- 2014/11/21 CRDT- 2014/12/03 06:00 PHST- 2013/10/15 00:00 [received] PHST- 2013/11/21 00:00 [revised] PHST- 2014/11/03 00:00 [accepted] PHST- 2014/12/03 06:00 [entrez] PHST- 2014/12/03 06:00 [pubmed] PHST- 2015/03/07 06:00 [medline] PHST- 2014/11/21 00:00 [pmc-release] AID - S0042-6822(14)00495-4 [pii] AID - 10.1016/j.virol.2014.11.001 [doi] PST - ppublish SO - Virology. 2015 Jan 15;475:15-27. doi: 10.1016/j.virol.2014.11.001. Epub 2014 Nov 21. PMID- 24769571 OWN - NLM STAT- MEDLINE DCOM- 20150413 LR - 20191112 IS - 0042-6857 (Print) IS - 0042-6857 (Linking) VI - 63 IP - 1 DP - 2013 TI - [A novel coronavirus, MERS-CoV]. PG - 1-6 AB - A novel human coronavirus was identified in Saudi Arabia and Qatar as the causative agent of severe acute respiratory diseases in 2012. The virus was termed Middle East Respiratory Syndrome Coronavirus (MERS-CoV) and is taken notice of important coronavirus caused severe diseases to human after the outbreak of severe acute respiratory syndrome (SARS) coronavirus. There is a lot of unknown characterization regarding MERS-CoV because of less than one year after finding the first case. MERS-CoV was related to the 2C betacoronavirus clade and is closely related to Tylonycteris bat coronavirus HKU4 and Pipistrellus bat coronavirus HKU5. Thus, bats are thought to be natural hosts of this virus. Recently, there were reports supposed to be cases of human to human infection. There are growing concerns about spread of infection. FAU - Mizutani, Tetsuya AU - Mizutani T AD - Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Uirusu JT - Uirusu JID - 0417475 SB - IM MH - Animals MH - Chiroptera/virology MH - *Coronavirus/genetics MH - Coronavirus Infections/epidemiology/transmission/*virology MH - Disease Outbreaks MH - Humans MH - Phylogeny MH - Severe Acute Respiratory Syndrome MH - Zoonoses EDAT- 2013/01/01 00:00 MHDA- 2015/04/14 06:00 CRDT- 2014/04/29 06:00 PHST- 2014/04/29 06:00 [entrez] PHST- 2013/01/01 00:00 [pubmed] PHST- 2015/04/14 06:00 [medline] AID - DN/JST.JSTAGE/jsv/63.1 [pii] AID - 10.2222/jsv.63.1 [doi] PST - ppublish SO - Uirusu. 2013;63(1):1-6. doi: 10.2222/jsv.63.1. PMID- 28549438 OWN - NLM STAT- MEDLINE DCOM- 20180221 LR - 20181113 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 14 IP - 1 DP - 2017 May 26 TI - Detection of alpha- and betacoronaviruses in rodents from Yunnan, China. PG - 98 LID - 10.1186/s12985-017-0766-9 [doi] LID - 98 AB - BACKGROUND: Rodents represent the most diverse mammals on the planet and are important reservoirs of human pathogens. Coronaviruses infect various animals, but to date, relatively few coronaviruses have been identified in rodents worldwide. The evolution and ecology of coronaviruses in rodent have not been fully investigated. RESULTS: In this study, we collected 177 intestinal samples from thress species of rodents in Jianchuan County, Yunnan Province, China. Alphacoronavirus and betacoronavirus were detected in 23 rodent samples from three species, namely Apodemus chevrieri (21/98), Eothenomys fidelis (1/62), and Apodemus ilex (1/17). We further characterized the full-length genome of an alphacoronavirus from the A. chevrieri rat and named it as AcCoV-JC34. The AcCoV-JC34 genome was 27,649 nucleotides long and showed a structure similar to the HKU2 bat coronavirus. Comparing the normal transcription regulatory sequence (TRS), 3 variant TRS sequences upstream the spike (S), ORF3, and ORF8 genes were found in the genome of AcCoV-JC34. In the conserved replicase domains, AcCoV-JC34 was most closely related to Rattus norvegicus coronavirus LNRV but diverged from other alphacoronaviruses, indicating that AcCoV-JC34 and LNRV may represent a novel alphacoronavirus species. However, the S and nucleocapsid proteins showed low similarity to those of LRNV, with 66.5 and 77.4% identities, respectively. Phylogenetic analysis revealed that the S genes of AcCoV-JC34, LRNV, and HKU2 formed a distinct lineage with all known coronaviruses. CONCLUSIONS: Both alphacoronaviruses and betacoronaviruses were detected in Apodemus chevrieri in the Yunnan Province of China, indicating that Apodemus chevrieri is an important host for coronavirus. Several new features were identified in the genome of an Apodemus chevrieri coronavirus. The phylogenetic distance to other coronaviruses suggests a variable origin and evolutionary route of the S genes of AcCoV-JC34, LRNV, and HKU2. These results indicate that the diversity of rodent coronaviruses is much higher than previously expected. Further surveillance and functional studies of these coronaviruses will help to better understand the importance of rodent as host for coronaviruses. FAU - Ge, Xing-Yi AU - Ge XY AD - Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - College of Biology, Hunan University, Changsha, 410082, China. FAU - Yang, Wei-Hong AU - Yang WH AD - Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. FAU - Zhou, Ji-Hua AU - Zhou JH AD - Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. FAU - Li, Bei AU - Li B AD - Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Zhang, Wei AU - Zhang W AD - Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Shi, Zheng-Li AU - Shi ZL AD - Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn. FAU - Zhang, Yun-Zhi AU - Zhang YZ AD - Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. zhangyunzhi1818@163.com. AD - School of Public Health, Dali University, Dali, 671000, China. zhangyunzhi1818@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170526 PL - England TA - Virol J JT - Virology journal JID - 101231645 SB - IM MH - Alphacoronavirus/classification/genetics/*isolation & purification MH - Animals MH - Arvicolinae/*virology MH - Betacoronavirus/classification/genetics/*isolation & purification MH - China MH - Coronavirus Infections/*veterinary/virology MH - Genes, Viral MH - Genetic Variation MH - Genome, Viral MH - Murinae/*virology MH - Phylogeny MH - Sequence Analysis, DNA PMC - PMC5446729 OTO - NOTNLM OT - Coronavirus OT - Genetic diversity OT - Rodent EDAT- 2017/05/28 06:00 MHDA- 2018/02/22 06:00 PMCR- 2017/05/26 CRDT- 2017/05/28 06:00 PHST- 2017/02/18 00:00 [received] PHST- 2017/05/19 00:00 [accepted] PHST- 2017/05/28 06:00 [entrez] PHST- 2017/05/28 06:00 [pubmed] PHST- 2018/02/22 06:00 [medline] PHST- 2017/05/26 00:00 [pmc-release] AID - 10.1186/s12985-017-0766-9 [pii] AID - 766 [pii] AID - 10.1186/s12985-017-0766-9 [doi] PST - epublish SO - Virol J. 2017 May 26;14(1):98. doi: 10.1186/s12985-017-0766-9. PMID- 29482919 OWN - NLM STAT- MEDLINE DCOM- 20180420 LR - 20221207 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 517 DP - 2018 Apr TI - SARS-CoV related Betacoronavirus and diverse Alphacoronavirus members found in western old-world. PG - 88-97 LID - S0042-6822(18)30020-5 [pii] LID - 10.1016/j.virol.2018.01.014 [doi] AB - The emergence of SARS-CoV and MERS-CoV, triggered the discovery of a high diversity of coronaviruses in bats. Studies from Europe have shown that coronaviruses circulate in bats in France but this reflects only a fraction of the whole diversity. In the current study the diversity of coronaviruses circulating in western Europe was extensively explored. Ten alphacoronaviruses in eleven bat species belonging to the Miniopteridae, Vespertilionidae and Rhinolophidae families and, a SARS-CoV-related Betacoronavirus in Rhinolophus ferrumequinum were identified. The diversity and prevalence of bat coronaviruses presently reported from western Europe is much higher than previously described and includes a SARS-CoV sister group. This diversity demonstrates the dynamic evolution and circulation of coronaviruses in this species. That said, the identified coronaviruses were consistently associated with a particular bat species or genus, and these relationships were maintained no matter the geographic location. The observed phylogenetic grouping of coronaviruses from the same species in Europe and Asia, emphasizes the role of host/pathogen coevolution in this group. CI - Copyright © 2018 Elsevier Inc. All rights reserved. FAU - Ar Gouilh, Meriadeg AU - Ar Gouilh M AD - Institut Pasteur, Unité Environnement et Risques Infectieux, CIBU, Infection et Epidemiologie, 75015, Paris, France; Normandie Université, EA2656, Groupe de Recherche sur l'Adaptation Microbienne, 14000, Caen, France. Electronic address: meriadeg.le-gouil@pasteur.fr. FAU - Puechmaille, Sébastien J AU - Puechmaille SJ AD - Greifswald University, 17489, Greifswald, Germany; University College Dublin, Belfield, Dublin 4, Ireland; Chauves-souris Aveyron, 12310, Vimenet, France. FAU - Diancourt, Laure AU - Diancourt L AD - Institut Pasteur, Unité Environnement et Risques Infectieux, CIBU, Infection et Epidemiologie, 75015, Paris, France. FAU - Vandenbogaert, Mathias AU - Vandenbogaert M AD - Institut Pasteur, Unité Environnement et Risques Infectieux, CIBU, Infection et Epidemiologie, 75015, Paris, France. FAU - Serra-Cobo, Jordi AU - Serra-Cobo J AD - IRBIO & Departament de de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain. FAU - Lopez Roïg, Marc AU - Lopez Roïg M AD - IRBIO & Departament de de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain. FAU - Brown, Paul AU - Brown P AD - French Agency for Food, Environmental and Occupational Health Safety (ANSES), Avian and Rabbit Virology Immunology and Parasitology Unit (VIPAC), Université Européenne de Bretagne, Ploufragan/Plouzané laboratory, 22440, Ploufragan, France. FAU - Moutou, François AU - Moutou F AD - Ecole Nationale Vétérinaire d'Alfort, 94704, Maison-Alfort, France. FAU - Caro, Valérie AU - Caro V AD - Institut Pasteur, Unité Environnement et Risques Infectieux, CIBU, Infection et Epidemiologie, 75015, Paris, France. FAU - Vabret, Astrid AU - Vabret A AD - Normandie Université, EA2656, Groupe de Recherche sur l'Adaptation Microbienne, 14000, Caen, France. FAU - Manuguerra, Jean-Claude AU - Manuguerra JC AD - Institut Pasteur, Unité Environnement et Risques Infectieux, CIBU, Infection et Epidemiologie, 75015, Paris, France. CN - EPICOREM consortium LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180223 PL - United States TA - Virology JT - Virology JID - 0110674 SB - IM MH - Alphacoronavirus/*genetics/isolation & purification MH - Animals MH - Betacoronavirus/*genetics/isolation & purification MH - Chiroptera/*virology MH - Coronavirus Infections/*veterinary/virology MH - Genetic Variation MH - Phylogeny MH - Severe acute respiratory syndrome-related coronavirus/*genetics PMC - PMC7112086 OTO - NOTNLM OT - Bats OT - Chiroptera OT - Coronavirus OT - Diversity OT - Emergence OT - Europe OT - Evolution OT - MERS-CoV OT - Phylogenetics OT - SARS-CoV EDAT- 2018/02/28 06:00 MHDA- 2018/04/21 06:00 PMCR- 2018/02/23 CRDT- 2018/02/28 06:00 PHST- 2017/10/09 00:00 [received] PHST- 2017/12/22 00:00 [revised] PHST- 2018/01/18 00:00 [accepted] PHST- 2018/02/28 06:00 [pubmed] PHST- 2018/04/21 06:00 [medline] PHST- 2018/02/28 06:00 [entrez] PHST- 2018/02/23 00:00 [pmc-release] AID - S0042-6822(18)30020-5 [pii] AID - 10.1016/j.virol.2018.01.014 [doi] PST - ppublish SO - Virology. 2018 Apr;517:88-97. doi: 10.1016/j.virol.2018.01.014. Epub 2018 Feb 23. PMID- 26483999 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20151020 LR - 20201001 IS - 2093-2340 (Print) IS - 2092-6448 (Electronic) IS - 1598-8112 (Linking) VI - 47 IP - 3 DP - 2015 Sep TI - Antiviral Treatment Guidelines for Middle East Respiratory Syndrome. PG - 212-22 LID - 10.3947/ic.2015.47.3.212 [doi] AB - Middle East respiratory syndrome (MERS) is an acute infectious disease of the respiratory system caused by the new betacoronavirus (MERS coronavirus, MERS-CoV), which shows high mortality rates. The typical symptoms of MERS are fever, cough, and shortness of breath, and it is often accompanied by pneumonia. The MERS-CoV was introduced to Republic of Korea in May 2015 by a patient returning from Saudi Arabia. The disease spread mostly through hospital infections, and by the time the epidemic ended in August, the total number of confirmed diagnoses was 186, among which 36 patients died. Reflecting the latest evidence for antiviral drugs in the treatment of MERS-CoV infection and the experiences of treating MERS patients in Republic of Korea, these guidelines focus on antiviral drugs to achieve effective treatment of MERS-CoV infections. FAU - Chong, Yong Pil AU - Chong YP AD - Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. FAU - Song, Joon Young AU - Song JY AD - Department of Infectious Diseases, Guro Hospital, Korea University College of Medicine, Seoul, Korea. FAU - Seo, Yu Bin AU - Seo YB AD - Department of Infectious Diseases, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea. FAU - Choi, Jae-Phil AU - Choi JP AD - Department of Infectious Diseases, Seoul Medical Center, Seoul, Korea. FAU - Shin, Hyoung-Shik AU - Shin HS AD - Center for Infectious Diseases, National Medical Center, Seoul, Korea. CN - Rapid Response Team LA - eng PT - Journal Article PT - Review DEP - 20150930 PL - Korea (South) TA - Infect Chemother JT - Infection & chemotherapy JID - 101531537 PMC - PMC4607778 OTO - NOTNLM OT - Antiviral OT - Coronavirus OT - MERS OT - Treatment EDAT- 2015/10/21 06:00 MHDA- 2015/10/21 06:01 PMCR- 2015/09/01 CRDT- 2015/10/21 06:00 PHST- 2015/08/31 00:00 [received] PHST- 2015/10/21 06:00 [entrez] PHST- 2015/10/21 06:00 [pubmed] PHST- 2015/10/21 06:01 [medline] PHST- 2015/09/01 00:00 [pmc-release] AID - 10.3947/ic.2015.47.3.212 [doi] PST - ppublish SO - Infect Chemother. 2015 Sep;47(3):212-22. doi: 10.3947/ic.2015.47.3.212. Epub 2015 Sep 30. PMID- 30176660 OWN - NLM STAT- MEDLINE DCOM- 20190123 LR - 20200425 IS - 1423-0100 (Electronic) IS - 0300-5526 (Print) IS - 0300-5526 (Linking) VI - 61 IP - 2 DP - 2018 TI - Three Main Inducers of Alphacoronavirus Infection of Enterocytes: Sialic Acid, Proteases, and Low pH. PG - 53-63 LID - 10.1159/000492424 [doi] AB - Transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) are similar coronaviruses, causing diseases characterized by vomiting, diarrhea, and death from severe dehydration in piglets. Thus, they have caused huge losses to the swine-breeding industry worldwide. Nowadays, they are easily transmitted among the continents via vehicles, equipment, and cargo. Both viruses establish an infection in porcine enterocytes in the small intestine, and their spike (S) proteins play a key role in the virus-cell binding process under unfavorable conditions when the intestine with a low pH is filled with a thick layer of mucus and proteases. Sialic acid, proteases, and low pH are three main inducers of coronavirus infection. However, the details of how sialic acid and low pH affect virus binding to the host cell are not determined, and the functions of the proteases are unknown. This review emphasizes the role of three factors in the invasion of TGEV and PEDV into porcine enterocytes and offers more insights into Alphacoronavirus infection in the intestinal environment. CI - © 2018 S. Karger AG, Basel. FAU - Yuan, Peng AU - Yuan P FAU - Yang, Zhou AU - Yang Z FAU - Song, Han AU - Song H FAU - Wang, Kai AU - Wang K FAU - Yang, Yang AU - Yang Y FAU - Xie, Luyi AU - Xie L FAU - Huang, Shilei AU - Huang S FAU - Liu, Jia AU - Liu J FAU - Ran, Lin AU - Ran L FAU - Song, Zhenhui AU - Song Z LA - eng PT - Journal Article PT - Review DEP - 20180903 PL - Switzerland TA - Intervirology JT - Intervirology JID - 0364265 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.- (Peptide Hydrolases) RN - GZP2782OP0 (N-Acetylneuraminic Acid) SB - IM MH - Alphacoronavirus/classification/*physiology MH - Animals MH - Coronavirus Infections/*metabolism/*virology MH - Enterocytes/*virology MH - *Host-Pathogen Interactions MH - Hydrogen-Ion Concentration MH - N-Acetylneuraminic Acid/metabolism MH - Peptide Hydrolases/metabolism MH - Phylogeny MH - Porcine epidemic diarrhea virus/physiology MH - Protein Binding MH - Receptors, Virus/metabolism MH - Spike Glycoprotein, Coronavirus/chemistry/metabolism MH - Structure-Activity Relationship MH - Swine MH - Swine Diseases/metabolism/virology MH - Transmissible gastroenteritis virus/physiology PMC - PMC7179561 OTO - NOTNLM OT - Epithelial cells OT - Low pH OT - Porcine epidemic diarrhea virus OT - Porcine small intestine OT - Protease OT - Sialic acid OT - Transmissible gastroenteritis virus COIS- No potential conflict of interest is reported by the authors. EDAT- 2018/09/04 06:00 MHDA- 2019/01/24 06:00 PMCR- 2020/04/23 CRDT- 2018/09/04 06:00 PHST- 2017/11/29 00:00 [received] PHST- 2018/07/19 00:00 [accepted] PHST- 2018/09/04 06:00 [pubmed] PHST- 2019/01/24 06:00 [medline] PHST- 2018/09/04 06:00 [entrez] PHST- 2020/04/23 00:00 [pmc-release] AID - 000492424 [pii] AID - int-0061-0053 [pii] AID - 10.1159/000492424 [doi] PST - ppublish SO - Intervirology. 2018;61(2):53-63. doi: 10.1159/000492424. Epub 2018 Sep 3. PMID- 25997928 OWN - NLM STAT- MEDLINE DCOM- 20160307 LR - 20200409 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 205 DP - 2015 Jul 2 TI - Bats as reservoirs of severe emerging infectious diseases. PG - 1-6 LID - S0168-1702(15)00177-X [pii] LID - 10.1016/j.virusres.2015.05.006 [doi] AB - In recent years severe infectious diseases have been constantly emerging, causing panic in the world. Now we know that many of these terrible diseases are caused by viruses originated from bats (Table 1), such as Ebola virus, Marburg, SARS coronavirus (SARS-CoV), MERS coronavirus (MERS-CoV), Nipah virus (NiV) and Hendra virus (HeV). These viruses have co-evolved with bats due to bats' special social, biological and immunological features. Although bats are not in close contact with humans, spillover of viruses from bats to intermediate animal hosts, such as horses, pigs, civets, or non-human primates, is thought to be the most likely mode to cause human infection. Humans may also become infected with viruses through aerosol by intruding into bat roosting caves or via direct contact with bats, such as catching bats or been bitten by bats. CI - Copyright © 2015 Elsevier B.V. All rights reserved. FAU - Han, Hui-Ju AU - Han HJ AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, China. Electronic address: 201413941@mail.sdu.edu.cn. FAU - Wen, Hong-ling AU - Wen HL AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, China. Electronic address: wenhonglingZ@sdu.edu.cn. FAU - Zhou, Chuan-Min AU - Zhou CM AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, China. Electronic address: zcmsdu@163.com. FAU - Chen, Fang-Fang AU - Chen FF AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, China. Electronic address: chenfangfang5683@163.com. FAU - Luo, Li-Mei AU - Luo LM AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, China. Electronic address: jmsllm@163.com. FAU - Liu, Jian-wei AU - Liu JW AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, China. Electronic address: liujw_2012@163.com. FAU - Yu, Xue-Jie AU - Yu XJ AD - School of Public Health, Shandong University, Jinan 250012, Shandong Province, China; Departments of Pathology and Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA; Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. Electronic address: xuyu@utmb.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20150518 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 SB - IM MH - Animals MH - Chiroptera/*virology MH - Communicable Diseases, Emerging/transmission/*virology MH - Disease Reservoirs/*virology MH - Humans MH - Virus Diseases/transmission/*virology MH - Viruses/classification/genetics/*isolation & purification PMC - PMC7132474 OTO - NOTNLM OT - Bat OT - Ebola OT - Emerging infectious diseases OT - Hendra OT - MERS OT - Natural reservoir OT - Nipah OT - SARS OT - Viruses EDAT- 2015/05/23 06:00 MHDA- 2016/03/08 06:00 PMCR- 2015/05/18 CRDT- 2015/05/23 06:00 PHST- 2015/03/14 00:00 [received] PHST- 2015/05/07 00:00 [revised] PHST- 2015/05/08 00:00 [accepted] PHST- 2015/05/23 06:00 [entrez] PHST- 2015/05/23 06:00 [pubmed] PHST- 2016/03/08 06:00 [medline] PHST- 2015/05/18 00:00 [pmc-release] AID - S0168-1702(15)00177-X [pii] AID - 10.1016/j.virusres.2015.05.006 [doi] PST - ppublish SO - Virus Res. 2015 Jul 2;205:1-6. doi: 10.1016/j.virusres.2015.05.006. Epub 2015 May 18. PMID- 27384656 OWN - NLM STAT- MEDLINE DCOM- 20170508 LR - 20191210 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 90 IP - 18 DP - 2016 Sep 15 TI - Porcine Epidemic Diarrhea Virus Infection Inhibits Interferon Signaling by Targeted Degradation of STAT1. PG - 8281-92 LID - 10.1128/JVI.01091-16 [doi] AB - Porcine epidemic diarrhea virus (PEDV) is a worldwide-distributed alphacoronavirus, but the pathogenesis of PEDV infection is not fully characterized. During virus infection, type I interferon (IFN) is a key mediator of innate antiviral responses. Most coronaviruses develop some strategy for at least partially circumventing the IFN response by limiting the production of IFN and by delaying the activation of the IFN response. However, the molecular mechanisms by which PEDV antagonizes the antiviral effects of interferon have not been fully characterized. Especially, how PEDV impacts IFN signaling components has yet to be elucidated. In this study, we observed that PEDV was relatively resistant to treatment with type I IFN. Western blot analysis showed that STAT1 expression was markedly reduced in PEDV-infected cells and that this reduction was not due to inhibition of STAT1 transcription. STAT1 downregulation was blocked by a proteasome inhibitor but not by an autophagy inhibitor, strongly implicating the ubiquitin-proteasome targeting degradation system. Since PEDV infection-induced STAT1 degradation was evident in cells pretreated with the general tyrosine kinase inhibitor, we conclude that STAT1 degradation is independent of the IFN signaling pathway. Furthermore, we report that PEDV-induced STAT1 degradation inhibits IFN-α signal transduction pathways. Pharmacological inhibition of STAT1 degradation rescued the ability of the host to suppress virus replication. Collectively, these data show that PEDV is capable of subverting the type I interferon response by inducing STAT1 degradation. IMPORTANCE: In this study, we show that PEDV is resistant to the antiviral effect of IFN. The molecular mechanism is the degradation of STAT1 by PEDV infection in a proteasome-dependent manner. This PEDV infection-induced STAT1 degradation contributes to PEDV replication. Our findings reveal a new mechanism evolved by PEDV to circumvent the host antiviral response. CI - Copyright © 2016, American Society for Microbiology. All Rights Reserved. FAU - Guo, Longjun AU - Guo L AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Luo, Xiaolei AU - Luo X AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Li, Ren AU - Li R AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Xu, Yunfei AU - Xu Y AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Zhang, Jian AU - Zhang J AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Ge, Jinying AU - Ge J AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Bu, Zhigao AU - Bu Z AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Feng, Li AU - Feng L AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Wang, Yue AU - Wang Y AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China wangyue@hvri.ac.cn. LA - eng PT - Journal Article DEP - 20160826 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Antiviral Agents) RN - 0 (Interferon-alpha) RN - 0 (STAT1 Transcription Factor) SB - IM MH - Animals MH - Antiviral Agents/*antagonists & inhibitors MH - Blotting, Western MH - Cell Line MH - Chlorocebus aethiops MH - Coronaviridae Infections MH - Down-Regulation MH - *Host-Pathogen Interactions MH - *Immune Evasion MH - Interferon-alpha/*antagonists & inhibitors MH - Porcine epidemic diarrhea virus/*pathogenicity MH - Proteolysis MH - STAT1 Transcription Factor/*antagonists & inhibitors/metabolism MH - Signal Transduction MH - Swine PMC - PMC5008104 EDAT- 2016/07/08 06:00 MHDA- 2017/05/10 06:00 PMCR- 2017/02/26 CRDT- 2016/07/08 06:00 PHST- 2016/06/04 00:00 [received] PHST- 2016/06/28 00:00 [accepted] PHST- 2017/02/26 00:00 [pmc-release] PHST- 2016/07/08 06:00 [entrez] PHST- 2016/07/08 06:00 [pubmed] PHST- 2017/05/10 06:00 [medline] AID - JVI.01091-16 [pii] AID - 01091-16 [pii] AID - 10.1128/JVI.01091-16 [doi] PST - epublish SO - J Virol. 2016 Aug 26;90(18):8281-92. doi: 10.1128/JVI.01091-16. Print 2016 Sep 15. PMID- 27255185 OWN - NLM STAT- MEDLINE DCOM- 20161213 LR - 20220321 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 13 DP - 2016 Jun 3 TI - Middle East Respiratory Syndrome Coronavirus (MERS-CoV) origin and animal reservoir. PG - 87 LID - 10.1186/s12985-016-0544-0 [doi] LID - 87 AB - Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) is a novel coronavirus discovered in 2012 and is responsible for acute respiratory syndrome in humans. Though not confirmed yet, multiple surveillance and phylogenetic studies suggest a bat origin. The disease is heavily endemic in dromedary camel populations of East Africa and the Middle East. It is unclear as to when the virus was introduced to dromedary camels, but data from studies that investigated stored dromedary camel sera and geographical distribution of involved dromedary camel populations suggested that the virus was present in dromedary camels several decades ago. Though bats and alpacas can serve as potential reservoirs for MERS-CoV, dromedary camels seem to be the only animal host responsible for the spill over human infections. FAU - Mohd, Hamzah A AU - Mohd HA AD - Saudi Ministry of Health, Riyadh, Saudi Arabia. FAU - Al-Tawfiq, Jaffar A AU - Al-Tawfiq JA AD - Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia. AD - Indiana University School of Medicine, Indianapolis, IN, USA. FAU - Memish, Ziad A AU - Memish ZA AD - Saudi Ministry of Health, Riyadh, Saudi Arabia. zmemish@yahoo.com. AD - College of Medicine, Alfaisal University, P.O. Box 54146, Riyadh, 11514, Kingdom of Saudi Arabia. zmemish@yahoo.com. LA - eng PT - Journal Article PT - Review DEP - 20160603 PL - England TA - Virol J JT - Virology journal JID - 101231645 SB - IM MH - Africa MH - Animals MH - Camelids, New World MH - Camelus MH - Chiroptera MH - Coronavirus Infections/*epidemiology/*veterinary/virology MH - *Disease Reservoirs MH - Disease Transmission, Infectious MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*genetics/*isolation & purification MH - Zoonoses/epidemiology/virology PMC - PMC4891877 OTO - NOTNLM OT - Animal OT - Bats OT - Camels OT - Coronavirus OT - Dromedary OT - MERS-CoV OT - Middle East OT - Middle East Respiratory Syndrome EDAT- 2016/06/04 06:00 MHDA- 2016/12/15 06:00 PMCR- 2016/06/03 CRDT- 2016/06/04 06:00 PHST- 2016/02/10 00:00 [received] PHST- 2016/05/18 00:00 [accepted] PHST- 2016/06/04 06:00 [entrez] PHST- 2016/06/04 06:00 [pubmed] PHST- 2016/12/15 06:00 [medline] PHST- 2016/06/03 00:00 [pmc-release] AID - 10.1186/s12985-016-0544-0 [pii] AID - 544 [pii] AID - 10.1186/s12985-016-0544-0 [doi] PST - epublish SO - Virol J. 2016 Jun 3;13:87. doi: 10.1186/s12985-016-0544-0. PMID- 26962326 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20160310 LR - 20200929 IS - 1751-0473 (Print) IS - 1751-0473 (Electronic) IS - 1751-0473 (Linking) VI - 11 DP - 2016 TI - Conserved antigenic sites between MERS-CoV and Bat-coronavirus are revealed through sequence analysis. PG - 3 LID - 10.1186/s13029-016-0049-7 [doi] LID - 3 AB - BACKGROUND: MERS-CoV is a newly emerged human coronavirus reported closely related with HKU4 and HKU5 Bat coronaviruses. Bat and MERS corona-viruses are structurally related. Therefore, it is of interest to estimate the degree of conserved antigenic sites among them. It is of importance to elucidate the shared antigenic-sites and extent of conservation between them to understand the evolutionary dynamics of MERS-CoV. RESULTS: Multiple sequence alignment of the spike (S), membrane (M), enveloped (E) and nucleocapsid (N) proteins was employed to identify the sequence conservation among MERS and Bat (HKU4, HKU5) coronaviruses. We used various in silico tools to predict the conserved antigenic sites. We found that MERS-CoV shared 30 % of its S protein antigenic sites with HKU4 and 70 % with HKU5 bat-CoV. Whereas 100 % of its E, M and N protein's antigenic sites are found to be conserved with those in HKU4 and HKU5. CONCLUSION: This sharing suggests that in case of pathogenicity MERS-CoV is more closely related to HKU5 bat-CoV than HKU4 bat-CoV. The conserved epitopes indicates their evolutionary relationship and ancestry of pathogenicity. FAU - Sharmin, Refat AU - Sharmin R AD - Research and Development Department, Incepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341 Bangladesh. FAU - Islam, Abul B M M K AU - Islam AB AD - Department of Genetic Engineering and Biotechnology, University of Dhaka, Science Complex Building, Dhaka, 1000 Bangladesh. LA - eng PT - Journal Article DEP - 20160309 PL - England TA - Source Code Biol Med JT - Source code for biology and medicine JID - 101276533 PMC - PMC4784407 OTO - NOTNLM OT - Epitope OT - HKU4 OT - HKU5 OT - MERS-CoV EDAT- 2016/03/11 06:00 MHDA- 2016/03/11 06:01 PMCR- 2016/03/09 CRDT- 2016/03/11 06:00 PHST- 2015/05/21 00:00 [received] PHST- 2016/02/26 00:00 [accepted] PHST- 2016/03/11 06:00 [entrez] PHST- 2016/03/11 06:00 [pubmed] PHST- 2016/03/11 06:01 [medline] PHST- 2016/03/09 00:00 [pmc-release] AID - 49 [pii] AID - 10.1186/s13029-016-0049-7 [doi] PST - epublish SO - Source Code Biol Med. 2016 Mar 9;11:3. doi: 10.1186/s13029-016-0049-7. eCollection 2016. PMID- 28110779 OWN - NLM STAT- MEDLINE DCOM- 20170227 LR - 20200407 IS - 1873-2542 (Electronic) IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 199 DP - 2017 Feb TI - Transmissible gastroenteritis virus does not suppress IFN-β induction but is sensitive to IFN in IPEC-J2 cells. PG - 128-134 LID - S0378-1135(16)30521-1 [pii] LID - 10.1016/j.vetmic.2016.12.031 [doi] AB - Coronaviruses tend to efficiently evade innate immune sensing. Alpha-coronaviruses interfere with the type I interferon (IFN) response in various ways, ensuring the limited activation of IFN responses. Transmissible gastroenteritis virus (TGEV), an Alphacoronavirus genera virus, is an important pathogen that mainly infects piglet, but little is known about the activation of the host immune response. We show that TGEV induces a delayed activation of the IFN response in intestinal epithelial cells. Briefly, IFN-β expression induced by TGEV infection is delayed with respect to that induced by poly(I:C) transfection. In addition, some of the IFN-stimulated genes (ISGs) were up-regulated in the early infection stage without obvious expression of IFN-β. Moreover, we show that activation of IFN responses induced by poly(I:C) could inhibit viral replication in the early infection stage, but failed in the late infection stage in IPEC-J2 cells. Finally, the activation of IFN responses induced by TGEV infection cannot inhibit viral replication. Taken together, this study provides a preliminary analysis of an interaction between TGEV and IFN-β responses of intestinal epithelial cells. CI - Copyright © 2016 Elsevier B.V. All rights reserved. FAU - Zhu, Liqi AU - Zhu L AD - Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. FAU - Yang, Xing AU - Yang X AD - Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. FAU - Mou, Chunxiao AU - Mou C AD - Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. FAU - Yang, Qian AU - Yang Q AD - Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China. Electronic address: zxbyq@njau.edu.cn. LA - eng PT - Journal Article DEP - 20161221 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Immunologic Factors) RN - 0 (Interferon Type I) RN - 0 (Receptors, Interferon) RN - 77238-31-4 (Interferon-beta) RN - O84C90HH2L (Poly I-C) SB - IM MH - Animals MH - Cell Line MH - Epithelial Cells/*immunology/*virology MH - Gastroenteritis, Transmissible, of Swine/*immunology/virology MH - Gene Expression Regulation/*immunology MH - Gene Knockout Techniques MH - Host-Pathogen Interactions/drug effects/*immunology MH - Immunologic Factors/pharmacology MH - Interferon Type I/immunology MH - Interferon-beta/genetics MH - Poly I-C/pharmacology MH - Receptors, Interferon/genetics MH - Swine MH - Transmissible gastroenteritis virus/*immunology MH - Virus Replication/drug effects PMC - PMC7117263 OTO - NOTNLM OT - IFN-β OT - ISGs OT - Intestinal epithelial cells OT - TGEV EDAT- 2017/01/24 06:00 MHDA- 2017/02/28 06:00 PMCR- 2016/12/21 CRDT- 2017/01/24 06:00 PHST- 2016/10/18 00:00 [received] PHST- 2016/12/19 00:00 [revised] PHST- 2016/12/19 00:00 [accepted] PHST- 2017/01/24 06:00 [entrez] PHST- 2017/01/24 06:00 [pubmed] PHST- 2017/02/28 06:00 [medline] PHST- 2016/12/21 00:00 [pmc-release] AID - S0378-1135(16)30521-1 [pii] AID - 10.1016/j.vetmic.2016.12.031 [doi] PST - ppublish SO - Vet Microbiol. 2017 Feb;199:128-134. doi: 10.1016/j.vetmic.2016.12.031. Epub 2016 Dec 21. PMID- 29070693 OWN - NLM STAT- MEDLINE DCOM- 20180723 LR - 20190319 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 4 DP - 2018 Feb 15 TI - Cryo-Electron Microscopy Structure of Porcine Deltacoronavirus Spike Protein in the Prefusion State. LID - 10.1128/JVI.01556-17 [doi] LID - e01556-17 AB - Coronavirus spike proteins from different genera are divergent, although they all mediate coronavirus entry into cells by binding to host receptors and fusing viral and cell membranes. Here, we determined the cryo-electron microscopy structure of porcine deltacoronavirus (PdCoV) spike protein at 3.3-Å resolution. The trimeric protein contains three receptor-binding S1 subunits that tightly pack into a crown-like structure and three membrane fusion S2 subunits that form a stalk. Each S1 subunit contains two domains, an N-terminal domain (S1-NTD) and C-terminal domain (S1-CTD). PdCoV S1-NTD has the same structural fold as alpha- and betacoronavirus S1-NTDs as well as host galectins, and it recognizes sugar as its potential receptor. PdCoV S1-CTD has the same structural fold as alphacoronavirus S1-CTDs, but its structure differs from that of betacoronavirus S1-CTDs. PdCoV S1-CTD binds to an unidentified receptor on host cell surfaces. PdCoV S2 is locked in the prefusion conformation by structural restraint of S1 from a different monomeric subunit. PdCoV spike possesses several structural features that may facilitate immune evasion by the virus, such as its compact structure, concealed receptor-binding sites, and shielded critical epitopes. Overall, this study reveals that deltacoronavirus spikes are structurally and evolutionally more closely related to alphacoronavirus spikes than to betacoronavirus spikes; it also has implications for the receptor recognition, membrane fusion, and immune evasion by deltacoronaviruses as well as coronaviruses in general. IMPORTANCE In this study, we determined the cryo-electron microscopy structure of porcine deltacoronavirus (PdCoV) spike protein at a 3.3-Å resolution. This is the first atomic structure of a spike protein from the deltacoronavirus genus, which is divergent in amino acid sequences from the well-studied alpha- and betacoronavirus spike proteins. Here, we described the overall structure of the PdCoV spike and the detailed structure of each of its structural elements. Moreover, we analyzed the functions of each of the structural elements. Based on the structures and functions of these structural elements, we discussed the evolution of PdCoV spike protein in relation to the spike proteins from other coronavirus genera. This study combines the structure, function, and evolution of PdCoV spike protein and provides many insights into its receptor recognition, membrane fusion, and immune evasion. CI - Copyright © 2017 American Society for Microbiology. FAU - Shang, Jian AU - Shang J AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Zheng, Yuan AU - Zheng Y AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Yang, Yang AU - Yang Y AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Liu, Chang AU - Liu C AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Geng, Qibin AU - Geng Q AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Tai, Wanbo AU - Tai W AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA. AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Du, Lanying AU - Du L AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA. FAU - Zhou, Yusen AU - Zhou Y AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China. FAU - Zhang, Wei AU - Zhang W AD - Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA zhangwei@umn.edu lifang@umn.edu. AD - Characterization Facility, College of Science and Engineering, University of Minnesota, Minneapolis, Minnesota, USA. FAU - Li, Fang AU - Li F AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA zhangwei@umn.edu lifang@umn.edu. LA - eng GR - R01 AI089728/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20180215 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM CIN - https://doi.org/10.1128/JVI.01628-17 MH - Animals MH - Coronavirus/*ultrastructure MH - *Cryoelectron Microscopy MH - Humans MH - Sf9 Cells MH - Spike Glycoprotein, Coronavirus/*ultrastructure MH - Spodoptera MH - Swine PMC - PMC5790952 EDAT- 2017/10/27 06:00 MHDA- 2018/07/24 06:00 PMCR- 2018/07/30 CRDT- 2017/10/27 06:00 PHST- 2018/10/25 00:00 [aheadofprint] PHST- 2017/10/27 06:00 [entrez] PHST- 2017/10/27 06:00 [pubmed] PHST- 2018/07/24 06:00 [medline] PHST- 2018/07/30 00:00 [pmc-release] AID - JVI.01556-17 [pii] AID - 01556-17 [pii] AID - 10.1128/JVI.01556-17 [doi] PST - epublish SO - J Virol. 2018 Feb 15;92(4):e01556-17. doi: 10.1128/JVI.01556-17. PMID- 30426315 OWN - NLM STAT- MEDLINE DCOM- 20190221 LR - 20210629 IS - 1572-994X (Electronic) IS - 0920-8569 (Print) IS - 0920-8569 (Linking) VI - 55 IP - 1 DP - 2019 Feb TI - Molecular identification of Betacoronavirus in bats from Sardinia (Italy): first detection and phylogeny. PG - 60-67 LID - 10.1007/s11262-018-1614-8 [doi] AB - Bats may be natural reservoirs for a large variety of emerging viruses, including mammalian coronaviruses (CoV). The recent emergence of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) in humans, with evidence that these viruses may have their ancestry in bats, highlights the importance of virus surveillance in bat populations. Here, we report the identification and molecular characterization of a bat β-Coronavirus, detected during a viral survey carried out on different bat species in the island of Sardinia (Italy). Cutaneous, oral swabs, and faecal samples were collected from 46 bats, belonging to 15 different species, and tested for viral presence. Coronavirus RNA was detected in faecal samples from three different species: the greater horseshoe bat (Rhinolophus ferrumequinum), the brown long-eared bat (Plecotus auritus), and the European free-tailed bat (Tadarida teniotis). Phylogenetic analyses based on RNA-dependent RNA polymerase (RdRp) sequences assigned the detected CoV to clade 2b within betacoronaviruses, clustering with SARS-like bat CoVs previously reported. These findings point to the need for continued surveillance of bat CoV circulating in Sardinian bats, and extend the current knowledge on CoV ecology with novel sequences detected in bat species not previously described as β-Coronavirus hosts. FAU - Lecis, Roberta AU - Lecis R AD - Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100, Sassari, Italy. rlecis@uniss.it. AD - Mediterranean Centre for Disease Control, University of Sassari, Via Vienna 2, 07100, Sassari, Italy. rlecis@uniss.it. FAU - Mucedda, Mauro AU - Mucedda M AD - Centro Pipistrelli Sardegna, Via G. Leopardi 1, 07100, Sassari, Italy. FAU - Pidinchedda, Ermanno AU - Pidinchedda E AD - Centro Pipistrelli Sardegna, Via G. Leopardi 1, 07100, Sassari, Italy. FAU - Pittau, Marco AU - Pittau M AD - Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100, Sassari, Italy. AD - Mediterranean Centre for Disease Control, University of Sassari, Via Vienna 2, 07100, Sassari, Italy. FAU - Alberti, Alberto AU - Alberti A AD - Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100, Sassari, Italy. AD - Mediterranean Centre for Disease Control, University of Sassari, Via Vienna 2, 07100, Sassari, Italy. LA - eng PT - Journal Article DEP - 20181113 PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 SB - IM EIN - Virus Genes. 2021 Oct;57(5):474. doi: 10.1007/s11262-021-01856-7. PMID: 34181161 MH - Animal Diseases/*virology MH - Animals MH - Betacoronavirus/*classification/*genetics/physiology MH - Chiroptera/*virology MH - Coronavirus Infections/*veterinary MH - Evolution, Molecular MH - Genome, Viral MH - Host Specificity MH - Humans MH - Italy MH - Phylogeny MH - Polymerase Chain Reaction MH - Sequence Analysis, DNA PMC - PMC7089328 OTO - NOTNLM OT - Bats OT - Coronavirus OT - RNA-dependent RNA polymerase OT - Rhinolophus ferrumequinum OT - Sardinia COIS- All authors declare that they have no conflict of interest. EDAT- 2018/11/15 06:00 MHDA- 2019/02/23 06:00 PMCR- 2020/03/23 CRDT- 2018/11/15 06:00 PHST- 2018/09/25 00:00 [received] PHST- 2018/11/08 00:00 [accepted] PHST- 2018/11/15 06:00 [pubmed] PHST- 2019/02/23 06:00 [medline] PHST- 2018/11/15 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s11262-018-1614-8 [pii] AID - 1614 [pii] AID - 10.1007/s11262-018-1614-8 [doi] PST - ppublish SO - Virus Genes. 2019 Feb;55(1):60-67. doi: 10.1007/s11262-018-1614-8. Epub 2018 Nov 13. PMID- 26847648 OWN - NLM STAT- MEDLINE DCOM- 20161213 LR - 20201209 IS - 1995-820X (Electronic) IS - 1674-0769 (Print) IS - 1995-820X (Linking) VI - 31 IP - 1 DP - 2016 Feb TI - Detection and characterization of diverse alpha- and betacoronaviruses from bats in China. PG - 69-77 LID - 10.1007/s12250-016-3727-3 [doi] AB - Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses (CoVs) were detected in 50 of 951 (positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase (RdRP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus. Among the 50 positive specimens, thirteen gave rise to CoV full-length RdRP gene amplification for further sequence comparison, of which three divergent sequences (two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses (JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus (JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed. FAU - Xu, Lin AU - Xu L AD - Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China. FAU - Zhang, Fuqiang AU - Zhang F AD - Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China. FAU - Yang, Weihong AU - Yang W AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. FAU - Jiang, Tinglei AU - Jiang T AD - School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China. FAU - Lu, Guanjun AU - Lu G AD - School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China. FAU - He, Biao AU - He B AD - Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China. FAU - Li, Xingyu AU - Li X AD - Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China. FAU - Hu, Tingsong AU - Hu T AD - Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China. FAU - Chen, Gang AU - Chen G AD - Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China. FAU - Feng, Yun AU - Feng Y AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. FAU - Zhang, Yuzhen AU - Zhang Y AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. FAU - Fan, Quanshui AU - Fan Q AD - Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China. FAU - Feng, Jiang AU - Feng J AD - School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China. FAU - Zhang, Hailin AU - Zhang H AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. FAU - Tu, Changchun AU - Tu C AD - Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China. changchun_tu@hotmail.com. AD - Jiangsu Co-innovation Center for Prevention and Control of Important, Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. changchun_tu@hotmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160202 PL - Netherlands TA - Virol Sin JT - Virologica Sinica JID - 101514185 RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Animals MH - Base Sequence MH - China MH - Chiroptera/*virology MH - Coronavirus/*classification/genetics MH - Coronavirus Infections/*veterinary/virology MH - Evolution, Molecular MH - *Genetic Variation MH - Genome, Viral MH - Phylogeny MH - RNA-Dependent RNA Polymerase/genetics MH - Real-Time Polymerase Chain Reaction/methods MH - Sequence Analysis PMC - PMC7090707 OTO - NOTNLM OT - Alphacoronavirus OT - Betacoronavirus OT - bats OT - diversity EDAT- 2016/02/06 06:00 MHDA- 2016/12/15 06:00 PMCR- 2017/02/02 CRDT- 2016/02/06 06:00 PHST- 2016/01/15 00:00 [received] PHST- 2016/01/21 00:00 [accepted] PHST- 2016/02/06 06:00 [entrez] PHST- 2016/02/06 06:00 [pubmed] PHST- 2016/12/15 06:00 [medline] PHST- 2017/02/02 00:00 [pmc-release] AID - 10.1007/s12250-016-3727-3 [pii] AID - 3727 [pii] AID - 10.1007/s12250-016-3727-3 [doi] PST - ppublish SO - Virol Sin. 2016 Feb;31(1):69-77. doi: 10.1007/s12250-016-3727-3. Epub 2016 Feb 2. PMID- 25841898 OWN - NLM STAT- MEDLINE DCOM- 20161213 LR - 20200407 IS - 1532-2971 (Electronic) IS - 1090-0233 (Print) IS - 1090-0233 (Linking) VI - 204 IP - 2 DP - 2015 May TI - Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis. PG - 134-43 LID - S1090-0233(15)00078-7 [pii] LID - 10.1016/j.tvjl.2015.02.017 [doi] AB - Porcine epidemic diarrhea virus (PEDV), a member of the genera Alphacoronavirus in the family Coronaviridae, causes acute diarrhea/vomiting, dehydration and high mortality in seronegative neonatal piglets. For the last three decades, PEDV infection has resulted in significant economic losses in the European and Asian pig industries, but in 2013-2014 the disease was also reported in the US, Canada and Mexico. The PED epidemic in the US, from April 2013 to the present, has led to the loss of more than 10% of the US pig population. The disappearance and re-emergence of epidemic PED indicates that the virus is able to escape from current vaccination protocols, biosecurity and control systems. Endemic PED is a significant problem, which is exacerbated by the emergence (or potential importation) of multiple PEDV variants. Epidemic PEDV strains spread rapidly and cause a high number of pig deaths. These strains are highly enteropathogenic and acutely infect villous epithelial cells of the entire small and large intestines although the jejunum and ileum are the primary sites. PEDV infections cause acute, severe atrophic enteritis accompanied by viremia that leads to profound diarrhea and vomiting, followed by extensive dehydration, which is the major cause of death in nursing piglets. A comprehensive understanding of the pathogenic characteristics of epidemic or endemic PEDV strains is needed to prevent and control the disease in affected regions and to develop an effective vaccine. This review focuses on the etiology, epidemiology, disease mechanisms and pathogenesis as well as immunoprophylaxis against PEDV infection. CI - Copyright © 2015 Elsevier Ltd. All rights reserved. FAU - Jung, Kwonil AU - Jung K AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. Electronic address: jung.221@osu.edu. FAU - Saif, Linda J AU - Saif LJ AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20150226 PL - England TA - Vet J JT - Veterinary journal (London, England : 1997) JID - 9706281 RN - 0 (Viral Vaccines) SB - IM CIN - Vet J. 2015 May;204(2):131. doi: 10.1016/j.tvjl.2015.03.019. PMID: 25913224 MH - Animals MH - Coronavirus Infections/epidemiology/prevention & control/*veterinary/virology MH - *Porcine epidemic diarrhea virus MH - Swine MH - Swine Diseases/epidemiology/prevention & control/*virology MH - Viral Vaccines/*immunology PMC - PMC7110711 OTO - NOTNLM OT - Disease OT - Pathogenesis OT - Porcine epidemic diarrhea virus OT - Review OT - Swine OT - Virus EDAT- 2015/04/07 06:00 MHDA- 2016/12/15 06:00 PMCR- 2015/02/26 CRDT- 2015/04/06 06:00 PHST- 2014/09/02 00:00 [received] PHST- 2015/02/16 00:00 [revised] PHST- 2015/02/18 00:00 [accepted] PHST- 2015/04/06 06:00 [entrez] PHST- 2015/04/07 06:00 [pubmed] PHST- 2016/12/15 06:00 [medline] PHST- 2015/02/26 00:00 [pmc-release] AID - S1090-0233(15)00078-7 [pii] AID - 10.1016/j.tvjl.2015.02.017 [doi] PST - ppublish SO - Vet J. 2015 May;204(2):134-43. doi: 10.1016/j.tvjl.2015.02.017. Epub 2015 Feb 26. PMID- 28104327 OWN - NLM STAT- MEDLINE DCOM- 20170310 LR - 20200409 IS - 1879-0070 (Electronic) IS - 0732-8893 (Print) IS - 0732-8893 (Linking) VI - 87 IP - 4 DP - 2017 Apr TI - Clinical validation of 3 commercial real-time reverse transcriptase polymerase chain reaction assays for the detection of Middle East respiratory syndrome coronavirus from upper respiratory tract specimens. PG - 320-324 LID - S0732-8893(17)30007-X [pii] LID - 10.1016/j.diagmicrobio.2017.01.003 [doi] AB - Since discovery of Middle East respiratory syndrome coronavirus (MERS-CoV), a novel betacoronavirus first isolated and characterized in 2012, MERS-CoV real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assays represent one of the most rapidly expanding commercial tests. However, in the absence of extensive evaluations of these assays on positive clinical material of different sources, evaluating their diagnostic effectiveness remains challenging. We describe the diagnostic performance evaluation of 3 common commercial MERS-CoV rRT-PCR assays on a large panel (n = 234) of upper respiratory tract specimens collected during an outbreak episode in Saudi Arabia. Assays were compared to the RealStar® MERS-CoV RT-PCR (Alton Diagnostics, Hamburg, Germany) assay as the gold standard. Results showed i) the TIB MolBiol® LightMix UpE and Orf1a assays (TIB MolBiol, Berlin, Germany) to be the most sensitive, followed by ii) the Anyplex™ Seegene MERS-CoV assay (Seegene, Seoul, Korea), and finally iii) the PrimerDesign™ Genesig® HCoV_2012 assay (PrimerDesign, England, United Kingdom). We also evaluate a modified protocol for the PrimerDesign™ Genesig® HCoV_2012 assay. CI - Copyright © 2017 Elsevier Inc. All rights reserved. FAU - Mohamed, Deqa H AU - Mohamed DH AD - Pathology/Microbiology Division, College of Medicine, King Saud University, Riyadh, Saudi Arabia. Electronic address: dmohamed@ksu.edu.sa. FAU - AlHetheel, AbdulKarim F AU - AlHetheel AF AD - Pathology/Microbiology Division, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Mohamud, Hanat S AU - Mohamud HS AD - Transfusion Microbiology Department, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Aldosari, Kamel AU - Aldosari K AD - Central Blood Bank, Riyadh, Saudi Arabia. FAU - Alzamil, Fahad A AU - Alzamil FA AD - Department of Pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Somily, Ali M AU - Somily AM AD - Pathology/Microbiology Division, College of Medicine, King Saud University, Riyadh, Saudi Arabia. LA - eng PT - Comparative Study PT - Journal Article DEP - 20170110 PL - United States TA - Diagn Microbiol Infect Dis JT - Diagnostic microbiology and infectious disease JID - 8305899 SB - IM MH - Coronavirus Infections/*microbiology MH - Disease Outbreaks MH - England MH - Germany MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*genetics MH - Real-Time Polymerase Chain Reaction/*methods MH - Republic of Korea MH - Respiratory System/*microbiology MH - Respiratory Tract Infections/*microbiology MH - Reverse Transcriptase Polymerase Chain Reaction/*methods MH - Saudi Arabia PMC - PMC7132760 OTO - NOTNLM OT - MERS-CoV OT - Orf1a OT - RT-PCR OT - Saudi Arabia OT - UpE EDAT- 2017/01/21 06:00 MHDA- 2017/03/11 06:00 PMCR- 2017/01/10 CRDT- 2017/01/21 06:00 PHST- 2016/09/13 00:00 [received] PHST- 2017/01/02 00:00 [revised] PHST- 2017/01/04 00:00 [accepted] PHST- 2017/01/21 06:00 [pubmed] PHST- 2017/03/11 06:00 [medline] PHST- 2017/01/21 06:00 [entrez] PHST- 2017/01/10 00:00 [pmc-release] AID - S0732-8893(17)30007-X [pii] AID - 10.1016/j.diagmicrobio.2017.01.003 [doi] PST - ppublish SO - Diagn Microbiol Infect Dis. 2017 Apr;87(4):320-324. doi: 10.1016/j.diagmicrobio.2017.01.003. Epub 2017 Jan 10. PMID- 27048154 OWN - NLM STAT- MEDLINE DCOM- 20170111 LR - 20200324 IS - 1612-9210 (Electronic) IS - 1612-9202 (Print) IS - 1612-9202 (Linking) VI - 13 IP - 1 DP - 2016 Mar TI - Coronavirus Infection and Diversity in Bats in the Australasian Region. PG - 72-82 LID - 10.1007/s10393-016-1116-x [doi] AB - Following the SARS outbreak, extensive surveillance was undertaken globally to detect and identify coronavirus diversity in bats. This study sought to identify the diversity and prevalence of coronaviruses in bats in the Australasian region. We identified four different genotypes of coronavirus, three of which (an alphacoronavirus and two betacoronaviruses) are potentially new species, having less than 90% nucleotide sequence identity with the most closely related described viruses. We did not detect any SARS-like betacoronaviruses, despite targeting rhinolophid bats, the putative natural host taxa. Our findings support the virus-host co-evolution hypothesis, with the detection of Miniopterus bat coronavirus HKU8 (previously reported in Miniopterus species in China, Hong Kong and Bulgaria) in Australian Miniopterus species. Similarly, we detected a novel betacoronavirus genotype from Pteropus alecto which is most closely related to Bat coronavirus HKU9 identified in other pteropodid bats in China, Kenya and the Philippines. We also detected possible cross-species transmission of bat coronaviruses, and the apparent enteric tropism of these viruses. Thus, our findings are consistent with a scenario wherein the current diversity and host specificity of coronaviruses reflects co-evolution with the occasional host shift. FAU - Smith, C S AU - Smith CS AD - School of Veterinary Science, The University of Queensland, Gatton, QLD, 4343, Australia. craig.smith@daf.qld.gov.au. AD - Department of Agriculture and Fisheries, Biosecurity Queensland, PO Box 156, Archerfield BC, Brisbane, QLD, 4108, Australia. craig.smith@daf.qld.gov.au. FAU - de Jong, C E AU - de Jong CE AD - Department of Agriculture and Fisheries, Biosecurity Queensland, PO Box 156, Archerfield BC, Brisbane, QLD, 4108, Australia. FAU - Meers, J AU - Meers J AD - School of Veterinary Science, The University of Queensland, Gatton, QLD, 4343, Australia. FAU - Henning, J AU - Henning J AD - School of Veterinary Science, The University of Queensland, Gatton, QLD, 4343, Australia. FAU - Wang, L- F AU - Wang L AD - Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore, 169857, Singapore. FAU - Field, H E AU - Field HE AD - Department of Agriculture and Fisheries, Biosecurity Queensland, PO Box 156, Archerfield BC, Brisbane, QLD, 4108, Australia. AD - EcoHealth Alliance, New York, NY, 10001, USA. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160405 PL - United States TA - Ecohealth JT - EcoHealth JID - 101222144 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Australasia/epidemiology MH - Base Sequence MH - Chiroptera/*virology MH - Coronavirus/genetics/*isolation & purification MH - Coronavirus Infections/epidemiology/veterinary/*virology MH - Genome, Viral MH - Genotype MH - Phylogeny MH - Polymerase Chain Reaction MH - Prevalence MH - RNA, Viral/genetics MH - Severe Acute Respiratory Syndrome/veterinary MH - Taiwan/epidemiology PMC - PMC7087777 OTO - NOTNLM OT - Asia OT - Australia OT - SARS OT - bat OT - coronavirus OT - diversity EDAT- 2016/04/07 06:00 MHDA- 2017/01/12 06:00 PMCR- 2020/03/23 CRDT- 2016/04/07 06:00 PHST- 2015/10/14 00:00 [received] PHST- 2016/03/06 00:00 [accepted] PHST- 2016/02/01 00:00 [revised] PHST- 2016/04/07 06:00 [entrez] PHST- 2016/04/07 06:00 [pubmed] PHST- 2017/01/12 06:00 [medline] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s10393-016-1116-x [pii] AID - 1116 [pii] AID - 10.1007/s10393-016-1116-x [doi] PST - ppublish SO - Ecohealth. 2016 Mar;13(1):72-82. doi: 10.1007/s10393-016-1116-x. Epub 2016 Apr 5. PMID- 29277555 OWN - NLM STAT- MEDLINE DCOM- 20190110 LR - 20240329 IS - 1567-7257 (Electronic) IS - 1567-1348 (Print) IS - 1567-1348 (Linking) VI - 58 DP - 2018 Mar TI - Distribution of bat-borne viruses and environment patterns. PG - 181-191 LID - S1567-1348(17)30435-5 [pii] LID - 10.1016/j.meegid.2017.12.009 [doi] AB - Environmental modifications are leading to biodiversity changes, loss and habitat disturbance. This in turn increases contacts between wildlife and hence the risk of transmission and emergence of zoonotic diseases. We analyzed the environment and land use using remote spatial data around the sampling locations of bats positive for coronavirus (21 sites) and astrovirus (11 sites) collected in 43 sites. A clear association between viruses and hosts was observed. Viruses associated to synanthropic bat genera, such as Myotis or Scotophilus were associated to highly transformed habitats with human presence while viruses associated to fruit bat genera were correlated with natural environments with dense forest, grassland areas and regions of high elevation. In particular, group C betacoronavirus were associated with mosaic habitats found in anthropized environments. CI - Copyright © 2017 Elsevier B.V. All rights reserved. FAU - Afelt, Aneta AU - Afelt A AD - Interdisciplinary Center for Mathematical and Computational Modelling, University of Warsaw, Tyniecka 15/17, 02-630 Warsaw, Poland. Electronic address: akafelt@uw.edu.pl. FAU - Lacroix, Audrey AU - Lacroix A AD - Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia; CIRAD, UMR 17, CIRAD-IRD, Montpellier, France. FAU - Zawadzka-Pawlewska, Urszula AU - Zawadzka-Pawlewska U AD - Institute of Physical Geography, Faculty of Geography and Regional Studies, University of Warsaw, Warsaw, Poland. FAU - Pokojski, Wojciech AU - Pokojski W AD - Laboratory of Spatial Information Systems, Faculty of Geography and Regional Studies, University of Warsaw, Warsaw, Poland. FAU - Buchy, Philippe AU - Buchy P AD - Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia; GSK Vaccines R&D, 150 Beach road, # 22-00, 189720, Singapore. FAU - Frutos, Roger AU - Frutos R AD - CIRAD, UMR 17, CIRAD-IRD, Montpellier, France; Université de Montpellier, IES, UMR 5214, CNRS-UM, Montpellier, France. LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20171223 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 SB - IM MH - Animals MH - Astroviridae/classification/genetics MH - Cambodia MH - Chiroptera/*virology MH - Cluster Analysis MH - Coronavirus/classification/genetics MH - *Environmental Microbiology MH - Genetic Variation MH - Humans MH - Laos MH - Mice MH - Phylogeny MH - Phylogeography MH - RNA Viruses MH - Zoonoses/*transmission/*virology PMC - PMC7106095 OTO - NOTNLM OT - Bat viruses OT - Cambodia OT - Emerging diseases OT - Environmental analysis OT - Evolution of environment OT - Lao PDR OT - South East Asia EDAT- 2017/12/27 06:00 MHDA- 2019/01/11 06:00 PMCR- 2017/12/23 CRDT- 2017/12/27 06:00 PHST- 2017/09/21 00:00 [received] PHST- 2017/12/08 00:00 [revised] PHST- 2017/12/12 00:00 [accepted] PHST- 2017/12/27 06:00 [pubmed] PHST- 2019/01/11 06:00 [medline] PHST- 2017/12/27 06:00 [entrez] PHST- 2017/12/23 00:00 [pmc-release] AID - S1567-1348(17)30435-5 [pii] AID - 10.1016/j.meegid.2017.12.009 [doi] PST - ppublish SO - Infect Genet Evol. 2018 Mar;58:181-191. doi: 10.1016/j.meegid.2017.12.009. Epub 2017 Dec 23. PMID- 28534504 OWN - NLM STAT- MEDLINE DCOM- 20181114 LR - 20181114 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 8 DP - 2017 May 23 TI - Crystal structure of the receptor binding domain of the spike glycoprotein of human betacoronavirus HKU1. PG - 15216 LID - 10.1038/ncomms15216 [doi] LID - 15216 AB - Human coronavirus (CoV) HKU1 is a pathogen causing acute respiratory illnesses and so far little is known about its biology. HKU1 virus uses its S1 subunit C-terminal domain (CTD) and not the N-terminal domain like other lineage A β-CoVs to bind to its yet unknown human receptor. Here we present the crystal structure of HKU1 CTD at 1.9 Å resolution. The structure consists of three subdomains: core, insertion and subdomain-1 (SD-1). While the structure of the core and SD-1 subdomains of HKU1 are highly similar to those of other β-CoVs, the insertion subdomain adopts a novel fold, which is largely invisible in the cryo-EM structure of the HKU1 S trimer. We identify five residues in the insertion subdomain that are critical for binding of neutralizing antibodies and two residues essential for receptor binding. Our study contributes to a better understanding of entry, immunity and evolution of CoV S proteins. FAU - Ou, Xiuyuan AU - Ou X AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. FAU - Guan, Hongxin AU - Guan H AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. FAU - Qin, Bo AU - Qin B AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. FAU - Mu, Zhixia AU - Mu Z AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. FAU - Wojdyla, Justyna A AU - Wojdyla JA AD - Swiss Light Source at Paul Scherrer Institute, Villigen CH-5232, Switzerland. FAU - Wang, Meitian AU - Wang M AD - Swiss Light Source at Paul Scherrer Institute, Villigen CH-5232, Switzerland. FAU - Dominguez, Samuel R AU - Dominguez SR AD - Departments of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado 80045, USA. FAU - Qian, Zhaohui AU - Qian Z AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. FAU - Cui, Sheng AU - Cui S AUID- ORCID: 0000-0001-6329-3582 AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170523 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Epitopes) RN - 0 (Mutant Proteins) RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Betacoronavirus/*metabolism MH - Cell Line MH - Coronavirus Infections/pathology/virology MH - Crystallography, X-Ray MH - Epitope Mapping MH - Epitopes/chemistry MH - Humans MH - Models, Molecular MH - Mutant Proteins/chemistry MH - Protein Binding MH - Protein Domains MH - Protein Multimerization MH - Receptors, Virus/*chemistry/*metabolism MH - Spike Glycoprotein, Coronavirus/*chemistry/*metabolism MH - Structural Homology, Protein MH - Virus Internalization PMC - PMC5529671 COIS- The authors declare no competing financial interests. EDAT- 2017/05/24 06:00 MHDA- 2018/11/15 06:00 PMCR- 2017/05/23 CRDT- 2017/05/24 06:00 PHST- 2016/09/29 00:00 [received] PHST- 2017/03/10 00:00 [accepted] PHST- 2017/05/24 06:00 [entrez] PHST- 2017/05/24 06:00 [pubmed] PHST- 2018/11/15 06:00 [medline] PHST- 2017/05/23 00:00 [pmc-release] AID - ncomms15216 [pii] AID - 10.1038/ncomms15216 [doi] PST - epublish SO - Nat Commun. 2017 May 23;8:15216. doi: 10.1038/ncomms15216. PMID- 26273575 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20150814 LR - 20240323 IS - 2287-3651 (Print) IS - 2287-366X (Electronic) IS - 2287-3651 (Linking) VI - 4 IP - 2 DP - 2015 Jul TI - Porcine epidemic diarrhea: a review of current epidemiology and available vaccines. PG - 166-76 LID - 10.7774/cevr.2015.4.2.166 [doi] AB - Porcine epidemic diarrhea virus (PEDV), an Alphacoronavirus in the family Coronaviridae, causes acute diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. PEDV can also cause diarrhea, agalactia, and abnormal reproductive cycles in pregnant sows. Although PEDV was first identified in Europe, it has resulted in significant economic losses in many Asian swine-raising countries, including Korea, China, Japan, Vietnam, and the Philippines. However, from April 2013 to the present, major outbreaks of PEDV have been reported in the United States, Canada, and Mexico. Moreover, intercontinental transmission of PEDV has increased mortality rates in seronegative neonatal piglets, resulting in 10% loss of the US pig population. The emergence and re-emergence of PEDV indicates that the virus is able to evade current vaccine strategies. Continuous emergence of multiple mutant strains from several regions has aggravated porcine epidemic diarrhea endemic conditions and highlighted the need for new vaccines based on the current circulating PEDV. Epidemic PEDV strains tend to be more pathogenic and cause increased death in pigs, thereby causing substantial financial losses for swine producers. In this review, we described the epidemiology of PEDV in several countries and present molecular characterization of current strains. We also discuss PEDV vaccines and related issues. FAU - Song, Daesub AU - Song D AD - Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Korea. FAU - Moon, Hyoungjoon AU - Moon H AD - Research Unit, Green Cross Veterinary Products, Yongin, Korea. FAU - Kang, Bokyu AU - Kang B AD - Research Unit, Green Cross Veterinary Products, Yongin, Korea. LA - eng PT - Journal Article PT - Review DEP - 20150729 PL - Korea (South) TA - Clin Exp Vaccine Res JT - Clinical and experimental vaccine research JID - 101592344 PMC - PMC4524901 OTO - NOTNLM OT - Epidemiology OT - Genetics OT - Porcine epidemic diarrhea OT - Vaccine COIS- No potential conflict of interest relevant to this article was reported. EDAT- 2015/08/15 06:00 MHDA- 2015/08/15 06:01 PMCR- 2015/07/01 CRDT- 2015/08/15 06:00 PHST- 2015/05/23 00:00 [received] PHST- 2015/06/20 00:00 [revised] PHST- 2015/06/25 00:00 [accepted] PHST- 2015/08/15 06:00 [entrez] PHST- 2015/08/15 06:00 [pubmed] PHST- 2015/08/15 06:01 [medline] PHST- 2015/07/01 00:00 [pmc-release] AID - 10.7774/cevr.2015.4.2.166 [doi] PST - ppublish SO - Clin Exp Vaccine Res. 2015 Jul;4(2):166-76. doi: 10.7774/cevr.2015.4.2.166. Epub 2015 Jul 29. PMID- 27059240 OWN - NLM STAT- MEDLINE DCOM- 20170315 LR - 20200324 IS - 1572-994X (Electronic) IS - 0920-8569 (Print) IS - 0920-8569 (Linking) VI - 52 IP - 4 DP - 2016 Aug TI - The close genetic relationship of lineage D Betacoronavirus from Nigerian and Kenyan straw-colored fruit bats (Eidolon helvum) is consistent with the existence of a single epidemiological unit across sub-Saharan Africa. PG - 573-7 LID - 10.1007/s11262-016-1331-0 [doi] AB - Straw-colored fruit bats (Eidolon helvum), which have been identified as natural hosts for several zoonotic pathogens, such as lyssaviruses, henipaviruses, and ebolavirus, are associated with human settlements in Nigeria where they are commonly consumed as a delicacy. However, information on the viruses harbored by these bats is scarce. In this study, coronavirus sequences were detected using a nested RT-PCR targeting 440 bp of the RNA-dependent RNA polymerase (RdRp) in six of 79 fecal samples collected from an urban colony of E. helvum in Ibadan, Nigeria. Phylogenetic analysis revealed that all six sequences were monophyletic and clustered in lineage D of Betacoronavirus. The extension of two fragments allowed us to classify our sequences within the RdRp Group Unit defined for Kenyan Betacoronavirus from the same host species. These findings are consistent with the previous suggestion on the existence of a single epidemiological unit of E. helvum across sub-Saharan Africa. This theory, which is supported by the genetic structure of continental E. helvum, could facilitate viral mixing between different colonies across the continent. FAU - Leopardi, Stefania AU - Leopardi S AD - National Reference Centre and OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita` 10, 35020, Legnaro, Padua, Italy. FAU - Oluwayelu, Daniel AU - Oluwayelu D AD - Department of Veterinary Microbiology and Parasitology, University of Ibadan, Ibadan, Nigeria. FAU - Meseko, Clement AU - Meseko C AD - Virology Department, National Veterinary Research Institute, Vom, Nigeria. FAU - Marciano, Sabrina AU - Marciano S AD - National Reference Centre and OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita` 10, 35020, Legnaro, Padua, Italy. FAU - Tassoni, Luca AU - Tassoni L AD - National Reference Centre and OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita` 10, 35020, Legnaro, Padua, Italy. FAU - Bakarey, Solomon AU - Bakarey S AD - Institute of Advanced Medical Research and Training, College of Medicine, University of Ibadan, Ibadan, Nigeria. FAU - Monne, Isabella AU - Monne I AD - National Reference Centre and OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita` 10, 35020, Legnaro, Padua, Italy. FAU - Cattoli, Giovanni AU - Cattoli G AD - National Reference Centre and OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita` 10, 35020, Legnaro, Padua, Italy. FAU - De Benedictis, Paola AU - De Benedictis P AD - National Reference Centre and OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita` 10, 35020, Legnaro, Padua, Italy. pdebenedictis@izsvenezie.it. LA - eng PT - Journal Article DEP - 20160408 PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronavirus/*genetics MH - Coronavirus Infections/virology MH - Kenya MH - Nigeria MH - Phylogeny MH - Zoonoses/virology PMC - PMC7089189 OTO - NOTNLM OT - Betacoronavirus OT - Epidemiological niche OT - Frugivorous bats OT - Nigeria COIS- All authors declare that they have no conflict of interest. EDAT- 2016/04/10 06:00 MHDA- 2017/03/16 06:00 PMCR- 2020/03/23 CRDT- 2016/04/10 06:00 PHST- 2015/11/23 00:00 [received] PHST- 2016/04/01 00:00 [accepted] PHST- 2016/04/10 06:00 [entrez] PHST- 2016/04/10 06:00 [pubmed] PHST- 2017/03/16 06:00 [medline] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s11262-016-1331-0 [pii] AID - 1331 [pii] AID - 10.1007/s11262-016-1331-0 [doi] PST - ppublish SO - Virus Genes. 2016 Aug;52(4):573-7. doi: 10.1007/s11262-016-1331-0. Epub 2016 Apr 8. PMID- 26935699 OWN - NLM STAT- MEDLINE DCOM- 20160321 LR - 20240328 IS - 1476-4687 (Electronic) IS - 0028-0836 (Print) IS - 0028-0836 (Linking) VI - 531 IP - 7592 DP - 2016 Mar 3 TI - Pre-fusion structure of a human coronavirus spike protein. PG - 118-21 LID - 10.1038/nature17200 [doi] AB - HKU1 is a human betacoronavirus that causes mild yet prevalent respiratory disease, and is related to the zoonotic SARS and MERS betacoronaviruses, which have high fatality rates and pandemic potential. Cell tropism and host range is determined in part by the coronavirus spike (S) protein, which binds cellular receptors and mediates membrane fusion. As the largest known class I fusion protein, its size and extensive glycosylation have hindered structural studies of the full ectodomain, thus preventing a molecular understanding of its function and limiting development of effective interventions. Here we present the 4.0 Å resolution structure of the trimeric HKU1 S protein determined using single-particle cryo-electron microscopy. In the pre-fusion conformation, the receptor-binding subunits, S1, rest above the fusion-mediating subunits, S2, preventing their conformational rearrangement. Surprisingly, the S1 C-terminal domains are interdigitated and form extensive quaternary interactions that occlude surfaces known in other coronaviruses to bind protein receptors. These features, along with the location of the two protease sites known to be important for coronavirus entry, provide a structural basis to support a model of membrane fusion mediated by progressive S protein destabilization through receptor binding and proteolytic cleavage. These studies should also serve as a foundation for the structure-based design of betacoronavirus vaccine immunogens. FAU - Kirchdoerfer, Robert N AU - Kirchdoerfer RN AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. FAU - Cottrell, Christopher A AU - Cottrell CA AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. FAU - Wang, Nianshuang AU - Wang N AD - Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755, USA. FAU - Pallesen, Jesper AU - Pallesen J AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. FAU - Yassine, Hadi M AU - Yassine HM AD - Viral Pathogenesis Laboratory, National Institute of Allergy and Infectious Diseases, Building 40, Room 2502, 40 Convent Drive, Bethesda, Maryland 20892, USA. FAU - Turner, Hannah L AU - Turner HL AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. FAU - Corbett, Kizzmekia S AU - Corbett KS AD - Viral Pathogenesis Laboratory, National Institute of Allergy and Infectious Diseases, Building 40, Room 2502, 40 Convent Drive, Bethesda, Maryland 20892, USA. FAU - Graham, Barney S AU - Graham BS AD - Viral Pathogenesis Laboratory, National Institute of Allergy and Infectious Diseases, Building 40, Room 2502, 40 Convent Drive, Bethesda, Maryland 20892, USA. FAU - McLellan, Jason S AU - McLellan JS AD - Department of Biochemistry, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire 03755, USA. FAU - Ward, Andrew B AU - Ward AB AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. LA - eng SI - PDB/5I08 GR - R56 AI118016/AI/NIAID NIH HHS/United States GR - Intramural NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, N.I.H., Intramural PT - Research Support, Non-U.S. Gov't PL - England TA - Nature JT - Nature JID - 0410462 RN - 0 (Protein Subunits) RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Viral Vaccines) SB - IM MH - Cell Line MH - Coronavirus/*chemistry/*ultrastructure MH - Cryoelectron Microscopy MH - Humans MH - Membrane Fusion MH - Models, Molecular MH - Protein Binding MH - Protein Multimerization MH - Protein Structure, Quaternary MH - Protein Structure, Tertiary MH - Protein Subunits/chemistry/metabolism MH - Proteolysis MH - Receptors, Virus/metabolism MH - Spike Glycoprotein, Coronavirus/*chemistry/metabolism/*ultrastructure MH - Viral Vaccines/chemistry/immunology MH - Virus Internalization PMC - PMC4860016 MID - NIHMS780460 COIS- The authors declare no competing financial interests. EDAT- 2016/03/05 06:00 MHDA- 2016/03/22 06:00 PMCR- 2020/03/25 CRDT- 2016/03/04 06:00 PHST- 2016/01/18 00:00 [received] PHST- 2016/02/05 00:00 [accepted] PHST- 2016/03/04 06:00 [entrez] PHST- 2016/03/05 06:00 [pubmed] PHST- 2016/03/22 06:00 [medline] PHST- 2020/03/25 00:00 [pmc-release] AID - nature17200 [pii] AID - BFnature17200 [pii] AID - 10.1038/nature17200 [doi] PST - ppublish SO - Nature. 2016 Mar 3;531(7592):118-21. doi: 10.1038/nature17200. PMID- 29434071 OWN - NLM STAT- MEDLINE DCOM- 20180914 LR - 20191210 IS - 0971-5916 (Print) IS - 0971-5916 (Linking) VI - 146 IP - 4 DP - 2017 Oct TI - Serosurvey of Malsoor virus among Rousettus leschenaulti bat & human population residing nearby Robber's cave, Mahabaleshwar, Maharashtra, India. PG - 545-547 LID - 10.4103/ijmr.IJMR_301_16 [doi] FAU - Yadav, Pragya AU - Yadav P AD - ICMR-National Institute of Virology, Pune 411 021, Maharashtra, India. FAU - Deoshatwar, Avinash AU - Deoshatwar A AD - ICMR-National Institute of Virology, Pune 411 021, Maharashtra, India. FAU - Shete, Anita AU - Shete A AD - ICMR-National Institute of Virology, Pune 411 021, Maharashtra, India. FAU - Tandale, Babasaheb AU - Tandale B AD - ICMR-National Institute of Virology, Pune 411 021, Maharashtra, India. FAU - Patil, Deepak AU - Patil D AD - ICMR-National Institute of Virology, Pune 411 021, Maharashtra, India. FAU - Dalal, Shital AU - Dalal S AD - ICMR-National Institute of Virology, Pune 411 021, Maharashtra, India. FAU - Mourya, Devendra AU - Mourya D AD - ICMR-National Institute of Virology, Pune 411 021, Maharashtra, India. LA - eng PT - Letter PL - India TA - Indian J Med Res JT - The Indian journal of medical research JID - 0374701 SB - IM MH - Adolescent MH - Aged MH - Animals MH - Betacoronavirus/genetics/*isolation & purification/pathogenicity MH - Child MH - Chiroptera/virology MH - Female MH - Humans MH - India/epidemiology MH - Infections/epidemiology/genetics/*transmission/*virology MH - Male MH - Phlebovirus/genetics/*isolation & purification/pathogenicity PMC - PMC5819039 COIS- None EDAT- 2018/02/13 06:00 MHDA- 2018/09/15 06:00 PMCR- 2017/10/01 CRDT- 2018/02/14 06:00 PHST- 2018/02/14 06:00 [entrez] PHST- 2018/02/13 06:00 [pubmed] PHST- 2018/09/15 06:00 [medline] PHST- 2017/10/01 00:00 [pmc-release] AID - IndianJMedRes_2017_146_4_545_224927 [pii] AID - IJMR-146-545 [pii] AID - 10.4103/ijmr.IJMR_301_16 [doi] PST - ppublish SO - Indian J Med Res. 2017 Oct;146(4):545-547. doi: 10.4103/ijmr.IJMR_301_16. PMID- 29102111 OWN - NLM STAT- MEDLINE DCOM- 20180702 LR - 20200407 IS - 1873-2542 (Electronic) IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 211 DP - 2017 Nov TI - Discovery of a novel swine enteric alphacoronavirus (SeACoV) in southern China. PG - 15-21 LID - S0378-1135(17)30940-9 [pii] LID - 10.1016/j.vetmic.2017.09.020 [doi] AB - Outbreaks of diarrhea in newborn piglets without detection of transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV), have been recorded in a pig farm in southern China since February 2017. Isolation and propagation of the pathogen in cell culture resulted in discovery of a novel swine enteric alphacoronavirus (tentatively named SeACoV) related to the bat coronavirus HKU2 identified in the same region a decade ago. Specific fluorescence signal was detected in Vero cells infected with SeACoV by using a positive sow serum collected in the same farm, but not by using TGEV-, PEDV- or PDCoV-specific antibody. Electron microscopy observation demonstrated that the virus particle with surface projections was 100-120nm in diameter. Complete genomic sequencing and analyses of SeACoV indicated that the extreme amino-terminal domain of the SeACoV spike (S) glycoprotein structurally similar to the domain 0 of the alphacoronavirus NL63, whereas the rest part of S structurally resembles domains B to D of the betacoronavirus. The SeACoV-S domain 0 associated with enteric tropism had an extremely high variability, harboring 75-amino-acid (aa) substitutions and a 2-aa insertion, compared to that of HKU2, which is likely responsible for the extended host range or cross-species transmission. The isolated virus was infectious in pigs when inoculated orally into 3-day-old newborn piglets, leading to clinical signs of diarrhea and fecal virus shedding. These results confirmed that it is a novel swine enteric coronavirus representing the fifth porcine coronavirus. CI - Copyright © 2017 Elsevier B.V. All rights reserved. FAU - Pan, Yongfei AU - Pan Y AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Hog Production Division, Guangdong Wen's Foodstuffs Group Co, Ltd, Xinxing, 527439, China. FAU - Tian, Xiaoyan AU - Tian X AD - Hog Production Division, Guangdong Wen's Foodstuffs Group Co, Ltd, Xinxing, 527439, China. FAU - Qin, Pan AU - Qin P AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China. FAU - Wang, Bin AU - Wang B AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China. FAU - Zhao, Pengwei AU - Zhao P AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China. FAU - Yang, Yong-Le AU - Yang YL AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China. FAU - Wang, Lianxiang AU - Wang L AD - Hog Production Division, Guangdong Wen's Foodstuffs Group Co, Ltd, Xinxing, 527439, China. FAU - Wang, Dongdong AU - Wang D AD - Hog Production Division, Guangdong Wen's Foodstuffs Group Co, Ltd, Xinxing, 527439, China. FAU - Song, Yanhua AU - Song Y AD - Hog Production Division, Guangdong Wen's Foodstuffs Group Co, Ltd, Xinxing, 527439, China. FAU - Zhang, Xiangbin AU - Zhang X AD - Hog Production Division, Guangdong Wen's Foodstuffs Group Co, Ltd, Xinxing, 527439, China; College of Animal Sciences, South China Agricultural University, Guangzhou 510642, China. Electronic address: zhangxb@scau.edu.cn. FAU - Huang, Yao-Wei AU - Huang YW AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China. Electronic address: yhuang@zju.edu.cn. LA - eng PT - Journal Article DEP - 20170928 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 SB - IM MH - Alphacoronavirus/genetics/*isolation & purification MH - Animals MH - Animals, Newborn MH - China/epidemiology MH - Chlorocebus aethiops MH - Coronavirus Infections/epidemiology/transmission/*veterinary/virology MH - Diarrhea/*veterinary/virology MH - Feces/virology MH - Female MH - Intestine, Small/virology MH - Swine MH - Swine Diseases/epidemiology/transmission/*virology MH - Vero Cells MH - Virus Shedding PMC - PMC7117260 OTO - NOTNLM OT - Bat OT - Cross-species transmission OT - Spike glycoprotein OT - Swine enteric alphacoronavirus (SeACoV) EDAT- 2017/11/06 06:00 MHDA- 2018/07/03 06:00 PMCR- 2017/09/28 CRDT- 2017/11/06 06:00 PHST- 2017/08/05 00:00 [received] PHST- 2017/09/27 00:00 [revised] PHST- 2017/09/27 00:00 [accepted] PHST- 2017/11/06 06:00 [entrez] PHST- 2017/11/06 06:00 [pubmed] PHST- 2018/07/03 06:00 [medline] PHST- 2017/09/28 00:00 [pmc-release] AID - S0378-1135(17)30940-9 [pii] AID - 10.1016/j.vetmic.2017.09.020 [doi] PST - ppublish SO - Vet Microbiol. 2017 Nov;211:15-21. doi: 10.1016/j.vetmic.2017.09.020. Epub 2017 Sep 28. PMID- 29887526 OWN - NLM STAT- MEDLINE DCOM- 20190213 LR - 20211204 IS - 1083-351X (Electronic) IS - 0021-9258 (Print) IS - 0021-9258 (Linking) VI - 293 IP - 30 DP - 2018 Jul 27 TI - Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells. PG - 11709-11726 LID - 10.1074/jbc.RA118.001897 [doi] AB - Coronavirus tropism is predominantly determined by the interaction between coronavirus spikes and the host receptors. In this regard, coronaviruses have evolved a complicated receptor-recognition system through their spike proteins. Spikes from highly related coronaviruses can recognize distinct receptors, whereas spikes of distant coronaviruses can employ the same cell-surface molecule for entry. Moreover, coronavirus spikes can recognize a broad range of cell-surface molecules in addition to the receptors and thereby can augment coronavirus attachment or entry. The receptor of Middle East respiratory syndrome coronavirus (MERS-CoV) is dipeptidyl peptidase 4 (DPP4). In this study, we identified membrane-associated 78-kDa glucose-regulated protein (GRP78) as an additional binding target of the MERS-CoV spike. Further analyses indicated that GRP78 could not independently render nonpermissive cells susceptible to MERS-CoV infection but could facilitate MERS-CoV entry into permissive cells by augmenting virus attachment. More importantly, by exploring potential interactions between GRP78 and spikes of other coronaviruses, we discovered that the highly conserved human GRP78 could interact with the spike protein of bat coronavirus HKU9 (bCoV-HKU9) and facilitate its attachment to the host cell surface. Taken together, our study has identified GRP78 as a host factor that can interact with the spike proteins of two Betacoronaviruses, the lineage C MERS-CoV and the lineage D bCoV-HKU9. The capacity of GRP78 to facilitate surface attachment of both a human coronavirus and a phylogenetically related bat coronavirus exemplifies the need for continuous surveillance of the evolution of animal coronaviruses to monitor their potential for human adaptations. CI - © 2018 Chu et al. FAU - Chu, Hin AU - Chu H AD - From the State Key Laboratory of Emerging Infectious Diseases. AD - Departments of Microbiology and. FAU - Chan, Che-Man AU - Chan CM AD - From the State Key Laboratory of Emerging Infectious Diseases. AD - Departments of Microbiology and. FAU - Zhang, Xi AU - Zhang X AD - Departments of Microbiology and. FAU - Wang, Yixin AU - Wang Y AD - Departments of Microbiology and. FAU - Yuan, Shuofeng AU - Yuan S AD - Departments of Microbiology and. FAU - Zhou, Jie AU - Zhou J AD - From the State Key Laboratory of Emerging Infectious Diseases. AD - Departments of Microbiology and. FAU - Au-Yeung, Rex Kwok-Him AU - Au-Yeung RK AD - Pathology. FAU - Sze, Kong-Hung AU - Sze KH AD - From the State Key Laboratory of Emerging Infectious Diseases. AD - Departments of Microbiology and. FAU - Yang, Dong AU - Yang D AD - Departments of Microbiology and. FAU - Shuai, Huiping AU - Shuai H AD - Departments of Microbiology and. FAU - Hou, Yuxin AU - Hou Y AD - Departments of Microbiology and. FAU - Li, Cun AU - Li C AD - Departments of Microbiology and. FAU - Zhao, Xiaoyu AU - Zhao X AD - Departments of Microbiology and. FAU - Poon, Vincent Kwok-Man AU - Poon VK AD - Departments of Microbiology and. FAU - Leung, Sze Pui AU - Leung SP AD - Departments of Microbiology and. FAU - Yeung, Man-Lung AU - Yeung ML AD - From the State Key Laboratory of Emerging Infectious Diseases. AD - Departments of Microbiology and. AD - Research Centre of Infection and Immunology. AD - Carol Yu Centre for Infection. FAU - Yan, Jinghua AU - Yan J AD - the CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101. FAU - Lu, Guangwen AU - Lu G AD - the West China Hospital Emergency Department, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, and. FAU - Jin, Dong-Yan AU - Jin DY AD - School of Biomedical Sciences, and. FAU - Gao, George Fu AU - Gao GF AD - the CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101. AD - the National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China. FAU - Chan, Jasper Fuk-Woo AU - Chan JF AD - From the State Key Laboratory of Emerging Infectious Diseases, jfwchan@hku.hk. AD - Departments of Microbiology and. AD - Research Centre of Infection and Immunology. AD - Carol Yu Centre for Infection. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - From the State Key Laboratory of Emerging Infectious Diseases, kyyuen@hku.hk. AD - Departments of Microbiology and. AD - Research Centre of Infection and Immunology. AD - Carol Yu Centre for Infection. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180610 PL - United States TA - J Biol Chem JT - The Journal of biological chemistry JID - 2985121R RN - 0 (Endoplasmic Reticulum Chaperone BiP) RN - 0 (HSPA5 protein, human) RN - 0 (Heat-Shock Proteins) RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.14.5 (DPP4 protein, human) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Animals MH - Betacoronavirus/*physiology MH - Cell Line MH - Chlorocebus aethiops MH - Coronavirus/*physiology MH - Coronavirus Infections/*metabolism MH - Dipeptidyl Peptidase 4/metabolism MH - Endoplasmic Reticulum Chaperone BiP MH - Heat-Shock Proteins/*metabolism MH - Host-Pathogen Interactions MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*physiology MH - Protein Interaction Maps MH - Receptors, Virus/metabolism MH - Spike Glycoprotein, Coronavirus/metabolism MH - Vero Cells MH - *Virus Attachment PMC - PMC6066311 OTO - NOTNLM OT - GRP78 OT - MERS-CoV OT - attachment factors OT - bat CoV-HKU9 OT - coronavirus OT - coronavirus spike OT - infection OT - infectious disease OT - viral attachment OT - viral evolution OT - viral infection OT - virology OT - virus COIS- The authors declare that they have no conflicts of interest with the contents of this article EDAT- 2018/06/12 06:00 MHDA- 2019/02/14 06:00 PMCR- 2019/07/27 CRDT- 2018/06/12 06:00 PHST- 2018/01/22 00:00 [received] PHST- 2018/05/26 00:00 [revised] PHST- 2018/06/12 06:00 [pubmed] PHST- 2019/02/14 06:00 [medline] PHST- 2018/06/12 06:00 [entrez] PHST- 2019/07/27 00:00 [pmc-release] AID - S0021-9258(20)31765-8 [pii] AID - RA118.001897 [pii] AID - 10.1074/jbc.RA118.001897 [doi] PST - ppublish SO - J Biol Chem. 2018 Jul 27;293(30):11709-11726. doi: 10.1074/jbc.RA118.001897. Epub 2018 Jun 10. PMID- 26924345 OWN - NLM STAT- MEDLINE DCOM- 20161031 LR - 20220330 IS - 2047-7732 (Electronic) IS - 2047-7724 (Print) IS - 2047-7724 (Linking) VI - 109 IP - 8 DP - 2015 TI - Middle East respiratory syndrome coronavirus (MERS-CoV): animal to human interaction. PG - 354-62 LID - 10.1080/20477724.2015.1122852 [doi] AB - The Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel enzootic betacoronavirus that was first described in September 2012. The clinical spectrum of MERS-CoV infection in humans ranges from an asymptomatic or mild respiratory illness to severe pneumonia and multi-organ failure; overall mortality is around 35.7%. Bats harbour several betacoronaviruses that are closely related to MERS-CoV but more research is needed to establish the relationship between bats and MERS-CoV. The seroprevalence of MERS-CoV antibodies is very high in dromedary camels in Eastern Africa and the Arabian Peninsula. MERS-CoV RNA and viable virus have been isolated from dromedary camels, including some with respiratory symptoms. Furthermore, near-identical strains of MERS-CoV have been isolated from epidemiologically linked humans and camels, confirming inter-transmission, most probably from camels to humans. Though inter-human spread within health care settings is responsible for the majority of reported MERS-CoV cases, the virus is incapable at present of causing sustained human-to-human transmission. Clusters can be readily controlled with implementation of appropriate infection control procedures. Phylogenetic and sequencing data strongly suggest that MERS-CoV originated from bat ancestors after undergoing a recombination event in the spike protein, possibly in dromedary camels in Africa, before its exportation to the Arabian Peninsula along the camel trading routes. MERS-CoV serosurveys are needed to investigate possible unrecognized human infections in Africa. Amongst the important measures to control MERS-CoV spread are strict regulation of camel movement, regular herd screening and isolation of infected camels, use of personal protective equipment by camel handlers and enforcing rules banning all consumption of unpasteurized camel milk and urine. FAU - Omrani, Ali S AU - Omrani AS AD - 1 Department of Medicine, Section of Infectious Diseases, King Faisal Specialist Hospital and Research Centre , Riyadh, Saudi Arabia. FAU - Al-Tawfiq, Jaffar A AU - Al-Tawfiq JA FAU - Memish, Ziad A AU - Memish ZA LA - eng PT - Journal Article PT - Review PL - England TA - Pathog Glob Health JT - Pathogens and global health JID - 101583421 SB - IM MH - Animals MH - Camelus/virology MH - Chiroptera/virology MH - Coronavirus Infections/transmission/*veterinary/*virology MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/genetics/isolation & purification/*physiology MH - Zoonoses/transmission/*virology PMC - PMC4809235 OTO - NOTNLM OT - Animal OT - Bat OT - Camel OT - Coronavirus OT - Dromedary OT - MERS-CoV OT - Middle East OT - Zoonosis EDAT- 2016/03/01 06:00 MHDA- 2016/11/01 06:00 PMCR- 2016/12/01 CRDT- 2016/03/01 06:00 PHST- 2016/03/01 06:00 [entrez] PHST- 2016/03/01 06:00 [pubmed] PHST- 2016/11/01 06:00 [medline] PHST- 2016/12/01 00:00 [pmc-release] AID - ypgh-109-354 [pii] AID - 10.1080/20477724.2015.1122852 [doi] PST - ppublish SO - Pathog Glob Health. 2015;109(8):354-62. doi: 10.1080/20477724.2015.1122852. PMID- 25410051 OWN - NLM STAT- MEDLINE DCOM- 20151116 LR - 20221207 IS - 1572-994X (Electronic) IS - 0920-8569 (Print) IS - 0920-8569 (Linking) VI - 50 IP - 1 DP - 2015 Feb TI - Acquisition of new protein domains by coronaviruses: analysis of overlapping genes coding for proteins N and 9b in SARS coronavirus. PG - 29-38 LID - 10.1007/s11262-014-1139-8 [doi] AB - Acquisition of new proteins by viruses usually occurs through horizontal gene transfer or through gene duplication, but another, less common mechanism is the usage of completely or partially overlapping reading frames. A case of acquisition of a completely new protein through introduction of a start codon in an alternative reading frame is the protein encoded by open reading frame (orf) 9b of SARS coronavirus. This gene completely overlaps with the nucleocapsid (N) gene (orf9a). Our findings indicate that the orf9b gene features a discordant codon-usage pattern. We analyzed the evolution of orf9b in concert with orf9a using sequence data of betacoronavirus-lineage b and found that orf9b, which encodes the overprinting protein, evolved largely independent of the overprinted orf9a. We also examined the protein products of these genomic sequences for their structural flexibility and found that it is not necessary for a newly acquired, overlapping protein product to be intrinsically disordered, in contrast to earlier suggestions. Our findings contribute to characterizing sequence properties of newly acquired genes making use of overlapping reading frames. FAU - Shukla, Aditi AU - Shukla A AD - Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany. FAU - Hilgenfeld, Rolf AU - Hilgenfeld R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141120 PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 RN - 0 (Coronavirus Nucleocapsid Proteins) RN - 0 (Nucleocapsid Proteins) RN - 0 (ORF-9b protein, SARS coronavirus) RN - 0 (Viral Proteins) SB - IM MH - Coronavirus Nucleocapsid Proteins MH - Genes, Overlapping MH - Genes, Viral MH - Humans MH - Models, Molecular MH - Nucleocapsid Proteins/*chemistry/*genetics MH - Protein Conformation MH - Protein Structure, Tertiary MH - Severe acute respiratory syndrome-related coronavirus/*chemistry/*genetics MH - Viral Proteins/*chemistry/*genetics PMC - PMC7089080 EDAT- 2014/11/21 06:00 MHDA- 2015/11/17 06:00 PMCR- 2020/03/23 CRDT- 2014/11/21 06:00 PHST- 2014/07/17 00:00 [received] PHST- 2014/10/25 00:00 [accepted] PHST- 2014/11/21 06:00 [entrez] PHST- 2014/11/21 06:00 [pubmed] PHST- 2015/11/17 06:00 [medline] PHST- 2020/03/23 00:00 [pmc-release] AID - 1139 [pii] AID - 10.1007/s11262-014-1139-8 [doi] PST - ppublish SO - Virus Genes. 2015 Feb;50(1):29-38. doi: 10.1007/s11262-014-1139-8. Epub 2014 Nov 20. PMID- 27217178 OWN - NLM STAT- MEDLINE DCOM- 20160808 LR - 20160524 IS - 0253-1933 (Print) IS - 0253-1933 (Linking) VI - 35 IP - 1 DP - 2016 Apr TI - Genomics and zoonotic infections: Middle East respiratory syndrome. PG - 191-202 LID - 10.20506/rst.35.1.2427 [doi] AB - The emergence of Middle East respiratory syndrome (MERS) and the discovery of MERS coronavirus (MERS-CoV) in 2012 suggests that another SARS-like epidemic is occurring. Unlike the severe acute respiratory syndrome (SARS) epidemic, which rapidly disappeared in less than one year, MERS has persisted for over three years. More than 1,600 cases of MERS have been reported worldwide, and the disease carries a worryingly high fatality rate of >30%. A total of 182 MERS-CoV genomes have been sequenced, including 94 from humans and 88 from dromedary camels. The 182 genomes all share >99% identity, indicating minimal variation among MERS-CoV genomes. MERS-CoV is a lineage C Betacoronavirus (ßCoV). MERS-CoV genomes can be roughly divided into two clades: clade A, which contains only a few strains, and clade B, to which most strains belong. In contrast to ORF1ab and structural proteins, the putative proteins encoded by ORF3, ORF4a, ORF4b, ORF5 and ORF8b in the MERS-CoV genome do not share homology with any known host or virus protein, other than those of its closely related lineage C ßCoVs. Human and dromedary viral genomes have intermingled, indicating that multiple camel-to-human transmission events have occurred. The multiple origins of MERS-CoV suggest that the virus has been resident in dromedaries for many years. This is consistent with the detection of anti-MERS-CoV antibodies in dromedary camels as early as the 1980s. FAU - Wernery, U AU - Wernery U FAU - Lau, S K P AU - Lau SK FAU - Woo, P C Y AU - Woo PC LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review PL - France TA - Rev Sci Tech JT - Revue scientifique et technique (International Office of Epizootics) JID - 8712301 SB - IM MH - Animals MH - Communicable Diseases, Emerging MH - Coronavirus Infections/epidemiology/mortality/*virology MH - Genome, Viral MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*genetics MH - Zoonoses/*virology OTO - NOTNLM OT - Coronavirus OT - Dromadaire OT - Genomique OT - Infection OT - Moyen-orient OT - Syndrome respiratoire du moyen-orient (mers) EDAT- 2016/05/25 06:00 MHDA- 2016/08/09 06:00 CRDT- 2016/05/25 06:00 PHST- 2016/05/25 06:00 [entrez] PHST- 2016/05/25 06:00 [pubmed] PHST- 2016/08/09 06:00 [medline] AID - 10.20506/rst.35.1.2427 [doi] PST - ppublish SO - Rev Sci Tech. 2016 Apr;35(1):191-202. doi: 10.20506/rst.35.1.2427. PMID- 25392474 OWN - NLM STAT- MEDLINE DCOM- 20150709 LR - 20190131 IS - 1471-2954 (Electronic) IS - 0962-8452 (Print) IS - 0962-8452 (Linking) VI - 282 IP - 1798 DP - 2015 Jan 7 TI - Ecological dynamics of emerging bat virus spillover. PG - 20142124 LID - 10.1098/rspb.2014.2124 [doi] LID - 20142124 AB - Viruses that originate in bats may be the most notorious emerging zoonoses that spill over from wildlife into domestic animals and humans. Understanding how these infections filter through ecological systems to cause disease in humans is of profound importance to public health. Transmission of viruses from bats to humans requires a hierarchy of enabling conditions that connect the distribution of reservoir hosts, viral infection within these hosts, and exposure and susceptibility of recipient hosts. For many emerging bat viruses, spillover also requires viral shedding from bats, and survival of the virus in the environment. Focusing on Hendra virus, but also addressing Nipah virus, Ebola virus, Marburg virus and coronaviruses, we delineate this cross-species spillover dynamic from the within-host processes that drive virus excretion to land-use changes that increase interaction among species. We describe how land-use changes may affect co-occurrence and contact between bats and recipient hosts. Two hypotheses may explain temporal and spatial pulses of virus shedding in bat populations: episodic shedding from persistently infected bats or transient epidemics that occur as virus is transmitted among bat populations. Management of livestock also may affect the probability of exposure and disease. Interventions to decrease the probability of virus spillover can be implemented at multiple levels from targeting the reservoir host to managing recipient host exposure and susceptibility. CI - © 2014 The Author(s) Published by the Royal Society. All rights reserved. FAU - Plowright, Raina K AU - Plowright RK AUID- ORCID: 0000-0002-3338-6590 AD - Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA Center for Infectious Disease Dynamics, Pennsylvania State University, State College, PA, USA raina.plowright@montana.edu. FAU - Eby, Peggy AU - Eby P AD - School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia. FAU - Hudson, Peter J AU - Hudson PJ AD - Center for Infectious Disease Dynamics, Pennsylvania State University, State College, PA, USA. FAU - Smith, Ina L AU - Smith IL AD - New and Emerging Zoonotic Diseases, CSIRO, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia. FAU - Westcott, David AU - Westcott D AD - CSIRO Ecosystem Sciences and Tropical Environment and Sustainability Sciences, James Cook University, Atherton, Queensland 4883, Australia. FAU - Bryden, Wayne L AU - Bryden WL AD - Equine Research Unit, School of Agriculture and Food Sciences, University of Queensland, Gatton, Queensland 4343, Australia. FAU - Middleton, Deborah AU - Middleton D AD - New and Emerging Zoonotic Diseases, CSIRO, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia. FAU - Reid, Peter A AU - Reid PA AD - Equine Veterinary Surgeon, Brisbane, Queensland 4034, Australia. FAU - McFarlane, Rosemary A AU - McFarlane RA AD - National Centre for Epidemiology and Population Health, Australian National University, Canberra 0200, Australia. FAU - Martin, Gerardo AU - Martin G AD - School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Townsville, Queensland 4811, Australia. FAU - Tabor, Gary M AU - Tabor GM AD - Center for Large Landscape Conservation, Bozeman, MT 59771, USA. FAU - Skerratt, Lee F AU - Skerratt LF AD - School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University, Townsville, Queensland 4811, Australia. FAU - Anderson, Dale L AU - Anderson DL AD - Equine Research Unit, School of Agriculture and Food Sciences, University of Queensland, Gatton, Queensland 4343, Australia. FAU - Crameri, Gary AU - Crameri G AD - New and Emerging Zoonotic Diseases, CSIRO, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia. FAU - Quammen, David AU - Quammen D AD - 414 South Third Avenue, Bozeman, MT 59715, USA. FAU - Jordan, David AU - Jordan D AD - New South Wales Department of Primary Industries, 1423 Bruxner Highway, Wollongbar, New South Wales 2477, Australia. FAU - Freeman, Paul AU - Freeman P AD - New South Wales Department of Primary Industries, 1423 Bruxner Highway, Wollongbar, New South Wales 2477, Australia. FAU - Wang, Lin-Fa AU - Wang LF AD - New and Emerging Zoonotic Diseases, CSIRO, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore 169857. FAU - Epstein, Jonathan H AU - Epstein JH AD - EcoHealth Alliance, New York, NY 10001, USA. FAU - Marsh, Glenn A AU - Marsh GA AD - New and Emerging Zoonotic Diseases, CSIRO, Australian Animal Health Laboratory, East Geelong, Victoria 3220, Australia. FAU - Kung, Nina Y AU - Kung NY AD - Animal Biosecurity and Welfare Program, Biosecurity Queensland, Department of Agriculture, Fisheries and Forestry, Brisbane, Queensland 4001, Australia. FAU - McCallum, Hamish AU - McCallum H AD - Griffith School of Environment, Griffith University, Brisbane 4111, Australia. LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Review PL - England TA - Proc Biol Sci JT - Proceedings. Biological sciences JID - 101245157 SB - IM MH - Animals MH - Chiroptera/*virology MH - Humans MH - *Models, Biological MH - Queensland MH - RNA Virus Infections/*transmission/virology MH - RNA Viruses/isolation & purification/*physiology MH - Zoonoses/*transmission/virology PMC - PMC4262174 OTO - NOTNLM OT - Ebola virus OT - Hendra virus in flying-foxes OT - Marburg virus OT - Nipah virus OT - emerging infectious diseases of bat origin OT - severe acute respiratory syndrome coronavirus EDAT- 2014/11/14 06:00 MHDA- 2015/07/15 06:00 PMCR- 2015/01/07 CRDT- 2014/11/14 06:00 PHST- 2014/11/14 06:00 [entrez] PHST- 2014/11/14 06:00 [pubmed] PHST- 2015/07/15 06:00 [medline] PHST- 2015/01/07 00:00 [pmc-release] AID - rspb.2014.2124 [pii] AID - rspb20142124 [pii] AID - 10.1098/rspb.2014.2124 [doi] PST - ppublish SO - Proc Biol Sci. 2015 Jan 7;282(1798):20142124. doi: 10.1098/rspb.2014.2124. PMID- 25019613 OWN - NLM STAT- MEDLINE DCOM- 20160101 LR - 20241105 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 9 IP - 7 DP - 2014 TI - Substitution at aspartic acid 1128 in the SARS coronavirus spike glycoprotein mediates escape from a S2 domain-targeting neutralizing monoclonal antibody. PG - e102415 LID - 10.1371/journal.pone.0102415 [doi] LID - e102415 AB - The Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) is the etiological agent for the infectious disease, SARS, which first emerged 10 years ago. SARS-CoV is a zoonotic virus that has crossed the species barriers to infect humans. Bats, which harbour a diverse pool of SARS-like CoVs (SL-CoVs), are believed to be the natural reservoir. The SARS-CoV surface Spike (S) protein is a major antigenic determinant in eliciting neutralizing antibody production during SARS-CoV infection. In our previous work, we showed that a panel of murine monoclonal antibodies (mAbs) that target the S2 subunit of the S protein are capable of neutralizing SARS-CoV infection in vitro (Lip KM et al, J Virol. 2006 Jan; 80(2): 941-50). In this study, we report our findings on the characterization of one of these mAbs, known as 1A9, which binds to the S protein at a novel epitope within the S2 subunit at amino acids 1111-1130. MAb 1A9 is a broadly neutralizing mAb that prevents viral entry mediated by the S proteins of human and civet SARS-CoVs as well as bat SL-CoVs. By generating mutant SARS-CoV that escapes the neutralization by mAb 1A9, the residue D1128 in S was found to be crucial for its interaction with mAb 1A9. S protein containing the substitution of D1128 with alanine (D1128A) exhibited a significant decrease in binding capability to mAb 1A9 compared to wild-type S protein. By using a pseudotyped viral entry assay, it was shown that the D1128A substitution in the escape virus allows it to overcome the viral entry blockage by mAb 1A9. In addition, the D1128A mutation was found to exert no effects on the S protein cell surface expression and incorporation into virion particles, suggesting that the escape virus retains the same viral entry property as the wild-type virus. FAU - Ng, Oi-Wing AU - Ng OW AD - Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore. FAU - Keng, Choong-Tat AU - Keng CT AD - Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Singapore. FAU - Leung, Cynthia Sau-Wai AU - Leung CS AD - Centre of Influenza Research, School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Peiris, J S Malik AU - Peiris JS AD - Centre of Influenza Research, School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Poon, Leo Lit Man AU - Poon LL AD - Centre of Influenza Research, School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Tan, Yee-Joo AU - Tan YJ AD - Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore; Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Singapore. LA - eng PT - Journal Article DEP - 20140714 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Antibodies, Monoclonal) RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 30KYC7MIAI (Aspartic Acid) SB - IM MH - Amino Acid Sequence MH - *Amino Acid Substitution MH - Animals MH - Antibodies, Monoclonal/physiology MH - Antibodies, Neutralizing/*physiology MH - Antibodies, Viral/physiology MH - Aspartic Acid/*chemistry MH - CHO Cells MH - Chiroptera/virology MH - Chlorocebus aethiops MH - Cricetulus MH - Epitope Mapping MH - HEK293 Cells MH - Humans MH - Mice, Inbred BALB C MH - Molecular Sequence Data MH - Neutralization Tests MH - Severe acute respiratory syndrome-related coronavirus/*genetics/metabolism/pathogenicity MH - Sequence Alignment MH - Spike Glycoprotein, Coronavirus/chemistry/*genetics/immunology MH - Vero Cells MH - Virion/genetics/pathogenicity MH - Viverridae/virology PMC - PMC4097068 COIS- Competing Interests: Leo Lit Man Poon is a PLOS ONE Editorial Board member. This does not alter the authors’ adherence to PLOS ONE Editorial policies and criteria. EDAT- 2014/07/16 06:00 MHDA- 2016/01/02 06:00 PMCR- 2014/07/14 CRDT- 2014/07/15 06:00 PHST- 2014/02/06 00:00 [received] PHST- 2014/06/19 00:00 [accepted] PHST- 2014/07/15 06:00 [entrez] PHST- 2014/07/16 06:00 [pubmed] PHST- 2016/01/02 06:00 [medline] PHST- 2014/07/14 00:00 [pmc-release] AID - PONE-D-14-05442 [pii] AID - 10.1371/journal.pone.0102415 [doi] PST - epublish SO - PLoS One. 2014 Jul 14;9(7):e102415. doi: 10.1371/journal.pone.0102415. eCollection 2014. PMID- 25484209 OWN - NLM STAT- MEDLINE DCOM- 20150330 LR - 20190108 IS - 2053-230X (Electronic) IS - 2053-230X (Linking) VI - 70 IP - Pt 12 DP - 2014 Dec 1 TI - Crystallization and preliminary crystallographic study of Feline infectious peritonitis virus main protease in complex with an inhibitor. PG - 1612-5 LID - 10.1107/S2053230X14022390 [doi] AB - Feline infectious peritonitis virus (FIPV) causes a lethal systemic granulomatous disease in wild and domestic cats around the world. Currently, no effective vaccines or drugs have been developed against it. As a member of the genus Alphacoronavirus, FIPV encodes two polyprotein precursors required for genome replication and transcription. Each polyprotein undergoes extensive proteolytic processing, resulting in functional subunits. This process is mainly mediated by its genome-encoded main protease, which is an attractive target for antiviral drug design. In this study, the main protease of FIPV in complex with a Michael acceptor-type inhibitor was crystallized. The complex crystals diffracted to 2.5 Å resolution and belonged to space group I422, with unit-cell parameters a = 112.3, b = 112.3, c = 102.1 Å. There is one molecule per asymmetric unit. FAU - Wang, Jinshan AU - Wang J AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Wang, Fenghua AU - Wang F AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Tan, Yusheng AU - Tan Y AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Chen, Xia AU - Chen X AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Zhao, Qi AU - Zhao Q AD - Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA. FAU - Fu, Sheng AU - Fu S AD - Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China. FAU - Li, Shuang AU - Li S AD - Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China. FAU - Chen, Cheng AU - Chen C AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. FAU - Yang, Haitao AU - Yang H AD - School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141114 PL - United States TA - Acta Crystallogr F Struct Biol Commun JT - Acta crystallographica. Section F, Structural biology communications JID - 101620319 RN - 0 (Protease Inhibitors) RN - EC 3.4.- (Peptide Hydrolases) SB - IM MH - Amino Acid Sequence MH - Coronavirus, Feline/*enzymology MH - Crystallization MH - Molecular Sequence Data MH - Peptide Hydrolases/*chemistry MH - Protease Inhibitors/*chemistry PMC - PMC4259223 OTO - NOTNLM OT - Feline infectious peritonitis virus OT - N3 inhibitor OT - main protease EDAT- 2014/12/09 06:00 MHDA- 2015/03/31 06:00 PMCR- 2016/12/01 CRDT- 2014/12/09 06:00 PHST- 2014/08/06 00:00 [received] PHST- 2014/10/11 00:00 [accepted] PHST- 2014/12/09 06:00 [entrez] PHST- 2014/12/09 06:00 [pubmed] PHST- 2015/03/31 06:00 [medline] PHST- 2016/12/01 00:00 [pmc-release] AID - S2053230X14022390 [pii] AID - no5064 [pii] AID - 10.1107/S2053230X14022390 [doi] PST - ppublish SO - Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1612-5. doi: 10.1107/S2053230X14022390. Epub 2014 Nov 14. PMID- 25433366 OWN - NLM STAT- MEDLINE DCOM- 20150916 LR - 20181113 IS - 1537-1719 (Electronic) IS - 0737-4038 (Print) IS - 0737-4038 (Linking) VI - 32 IP - 2 DP - 2015 Feb TI - How and why overcome the impediments to resolution: lessons from rhinolophid and hipposiderid bats. PG - 313-33 LID - 10.1093/molbev/msu329 [doi] AB - The phylogenetic and taxonomic relationships among the Old World leaf-nosed bats (Hipposideridae) and the closely related horseshoe bats (Rhinolophidae) remain unresolved. In this study, we generated a novel approximately 10-kb molecular data set of 19 nuclear exon and intron gene fragments for 40 bat species to elucidate the phylogenetic relationships within the families Rhinolophidae and Hipposideridae. We estimated divergence times and explored potential reasons for any incongruent phylogenetic signal. We demonstrated the effects of outlier taxa and genes on phylogenetic reconstructions and compared the relative performance of intron and exon data to resolve phylogenetic relationships. Phylogenetic analyses produced a well-resolved phylogeny, supporting the familial status of Hipposideridae and demonstrated the paraphyly of the largest genus, Hipposideros. A fossil-calibrated timetree and biogeographical analyses estimated that Rhinolophidae and Hipposideridae diverged in Africa during the Eocene approximately 42 Ma. The phylogram, the timetree, and a unique retrotransposon insertion supported the elevation of the subtribe Rhinonycterina to family level and which is diagnosed herein. Comparative analysis of diversification rates showed that the speciose genera Rhinolophus and Hipposideros underwent diversification during the Mid-Miocene Climatic Optimum. The intron versus exon analyses demonstrated the improved nodal support provided by introns for our optimal tree, an important finding for large-scale phylogenomic studies, which typically rely on exon data alone. With the recent outbreak of Middle East respiratory syndrome, caused by a novel coronavirus, the study of these species is urgent as they are considered the natural reservoir for emergent severe acute respiratory syndrome (SARS)-like coronaviruses. It has been shown that host phylogeny is the primary factor that determines a virus's persistence, replicative ability, and can act as a predictor of new emerging disease. Therefore, this newly resolved phylogeny can be used to direct future assessments of viral diversity and to elucidate the origin and development of SARS-like coronaviruses in mammals. CI - © The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. FAU - Foley, Nicole M AU - Foley NM AD - School of Biology & Environmental Science, University College Dublin, Belfield, Dublin, Ireland. FAU - Thong, Vu Dinh AU - Thong VD AD - Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam. FAU - Soisook, Pipat AU - Soisook P AD - Princess Maha Chakri Sirindhorn Natural History Museum, Prince of Songkla University, Hat Yai, Songkhla, Thailand. FAU - Goodman, Steven M AU - Goodman SM AD - Field Museum of Natural History, Chicago, IL, USA Association Vahatra, Antananarivo, Madagascar. FAU - Armstrong, Kyle N AU - Armstrong KN AD - Australian Centre for Evolutionary Biology & Biodiversity, The University of Adelaide, Adelaide, South Australia, Australia South Australian Museum, Adelaide, South Australia, Australia. FAU - Jacobs, David S AU - Jacobs DS AD - Department of Biological Sciences, University of Cape Town, Rondebosch, South Africa. FAU - Puechmaille, Sébastien J AU - Puechmaille SJ AD - School of Biology & Environmental Science, University College Dublin, Belfield, Dublin, Ireland Zoological Institute and Museum, Greifswald University, Greifswald, Germany emma.teeling@ucd.ie s.puechmaille@gmail.com. FAU - Teeling, Emma C AU - Teeling EC AD - School of Biology & Environmental Science, University College Dublin, Belfield, Dublin, Ireland emma.teeling@ucd.ie s.puechmaille@gmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141129 PL - United States TA - Mol Biol Evol JT - Molecular biology and evolution JID - 8501455 SB - IM MH - Animals MH - Chiroptera/*classification/*genetics MH - Exons/genetics MH - Introns/genetics MH - Mammals/classification/genetics MH - Phylogeny PMC - PMC4769323 OTO - NOTNLM OT - Rhinonycteridae OT - biogeography OT - exon versus intron OT - mammals OT - phylogenetics OT - virus EDAT- 2014/11/30 06:00 MHDA- 2015/09/17 06:00 PMCR- 2014/11/29 CRDT- 2014/11/30 06:00 PHST- 2014/11/30 06:00 [entrez] PHST- 2014/11/30 06:00 [pubmed] PHST- 2015/09/17 06:00 [medline] PHST- 2014/11/29 00:00 [pmc-release] AID - msu329 [pii] AID - 10.1093/molbev/msu329 [doi] PST - ppublish SO - Mol Biol Evol. 2015 Feb;32(2):313-33. doi: 10.1093/molbev/msu329. Epub 2014 Nov 29. PMID- 25378498 OWN - NLM STAT- MEDLINE DCOM- 20150413 LR - 20181113 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 2 DP - 2015 Jan 15 TI - Activation of the chicken type I interferon response by infectious bronchitis coronavirus. PG - 1156-67 LID - 10.1128/JVI.02671-14 [doi] AB - Coronaviruses from both the Alphacoronavirus and Betacoronavirus genera interfere with the type I interferon (IFN) response in various ways, ensuring the limited activation of the IFN response in most cell types. Of the gammacoronaviruses that mainly infect birds, little is known about the activation of the host immune response. We show that the prototypical Gammacoronavirus, infectious bronchitis virus (IBV), induces a delayed activation of the IFN response in primary renal cells, tracheal epithelial cells, and a chicken cell line. In fact, Ifnβ expression is delayed with respect to the peak of viral replication and the accompanying accumulation of double-stranded RNA (dsRNA). In addition, we demonstrate that MDA5 is the primary sensor for Gammacoronavirus infections in chicken cells. Furthermore, we provide evidence that accessory proteins 3a and 3b of IBV modulate the response at the transcriptional and translational levels. Finally, we show that, despite the lack of activation of the IFN response during the early phase of IBV infection, the signaling of nonself dsRNA through both MDA5 and TLR3 remains intact in IBV-infected cells. Taken together, this study provides the first comprehensive analysis of host-virus interactions of a Gammacoronavirus with avian innate immune responses. IMPORTANCE: Our results demonstrate that IBV has evolved multiple strategies to avoid the activation of the type I interferon response. Taken together, the present study closes a gap in the understanding of host-IBV interaction and paves the way for further characterization of the mechanisms underlying immune evasion strategies as well as the pathogenesis of gammacoronaviruses. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Kint, Joeri AU - Kint J AD - Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands MSD Animal Health, Bioprocess Technology & Support, Boxmeer, The Netherlands. FAU - Fernandez-Gutierrez, Marcela AU - Fernandez-Gutierrez M AD - Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands. FAU - Maier, Helena J AU - Maier HJ AD - Avian Viral Diseases, The Pirbright Institute, Compton Laboratory, United Kingdom. FAU - Britton, Paul AU - Britton P AD - Avian Viral Diseases, The Pirbright Institute, Compton Laboratory, United Kingdom. FAU - Langereis, Martijn A AU - Langereis MA AD - Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. FAU - Koumans, Joseph AU - Koumans J AD - MSD Animal Health, Bioprocess Technology & Support, Boxmeer, The Netherlands. FAU - Wiegertjes, Geert F AU - Wiegertjes GF AD - Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands. FAU - Forlenza, Maria AU - Forlenza M AUID- ORCID: 0000-0001-9026-7320 AD - Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands maria.forlenza@wur.nl. LA - eng GR - Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141105 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Interferon Type I) RN - 0 (RNA, Viral) RN - 0 (Receptors, Immunologic) RN - EC 3.6.4.13 (DEAD-box RNA Helicases) SB - IM MH - Animals MH - Cells, Cultured MH - Chickens MH - DEAD-box RNA Helicases/immunology/metabolism MH - Epithelial Cells/immunology/virology MH - *Host-Pathogen Interactions MH - Infectious bronchitis virus/*immunology MH - Interferon Type I/*biosynthesis/*immunology MH - RNA, Viral/immunology/metabolism MH - Receptors, Immunologic PMC - PMC4300645 EDAT- 2014/11/08 06:00 MHDA- 2015/04/14 06:00 PMCR- 2015/07/15 CRDT- 2014/11/08 06:00 PHST- 2014/11/08 06:00 [entrez] PHST- 2014/11/08 06:00 [pubmed] PHST- 2015/04/14 06:00 [medline] PHST- 2015/07/15 00:00 [pmc-release] AID - JVI.02671-14 [pii] AID - 02671-14 [pii] AID - 10.1128/JVI.02671-14 [doi] PST - ppublish SO - J Virol. 2015 Jan 15;89(2):1156-67. doi: 10.1128/JVI.02671-14. Epub 2014 Nov 5. PMID- 29273042 OWN - NLM STAT- MEDLINE DCOM- 20180709 LR - 20181113 IS - 1746-6148 (Electronic) IS - 1746-6148 (Linking) VI - 13 IP - 1 DP - 2017 Dec 22 TI - Coronavirus and paramyxovirus in bats from Northwest Italy. PG - 396 LID - 10.1186/s12917-017-1307-x [doi] LID - 396 AB - BACKGROUND: Bat-borne virus surveillance is necessary for determining inter-species transmission risks and is important due to the wide-range of bat species which may harbour potential pathogens. This study aimed to monitor coronaviruses (CoVs) and paramyxoviruses (PMVs) in bats roosting in northwest Italian regions. Our investigation was focused on CoVs and PMVs due to their proven ability to switch host and their zoonotic potential. Here we provide the phylogenetic characterization of the highly conserved polymerase gene fragments. RESULTS: Family-wide PCR screenings were used to test 302 bats belonging to 19 different bat species. Thirty-eight animals from 12 locations were confirmed as PCR positive, with an overall detection rate of 12.6% [95% CI: 9.3-16.8]. CoV RNA was found in 36 bats belonging to eight species, while PMV RNA in three Pipistrellus spp. Phylogenetic characterization have been obtained for 15 alpha- CoVs, 5 beta-CoVs and three PMVs; moreover one P. pipistrellus resulted co-infected with both CoV and PMV. A divergent alpha-CoV clade from Myotis nattereri SpA is also described. The compact cluster of beta-CoVs from R. ferrumequinum roosts expands the current viral sequence database, specifically for this species in Europe. To our knowledge this is the first report of CoVs in Plecotus auritus and M. oxygnathus, and of PMVs in P. kuhlii. CONCLUSIONS: This study identified alpha and beta-CoVs in new bat species and in previously unsurveyed Italian regions. To our knowledge this represents the first and unique report of PMVs in Italy. The 23 new bat genetic sequences presented will expand the current molecular bat-borne virus databases. Considering the amount of novel bat-borne PMVs associated with the emergence of zoonotic infections in animals and humans in the last years, the definition of viral diversity within European bat species is needed. Performing surveillance studies within a specific geographic area can provide awareness of viral burden where bats roost in close proximity to spillover hosts, and form the basis for the appropriate control measures against potential threats for public health and optimal management of bats and their habitats. FAU - Rizzo, Francesca AU - Rizzo F AUID- ORCID: 0000-0001-7659-1773 AD - Istituto zooprofilattico sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10148, Torino, Italy. francesca.rizzo@izsto.it. FAU - Edenborough, Kathryn M AU - Edenborough KM AD - Robert Koch Institute, Seestraße 10, 13353, Berlin, Germany. FAU - Toffoli, Roberto AU - Toffoli R AD - Chirosphera, via Tetti Barbiere 11, 10026, Santena, TO, Italy. FAU - Culasso, Paola AU - Culasso P AD - Chirosphera, via Tetti Barbiere 11, 10026, Santena, TO, Italy. FAU - Zoppi, Simona AU - Zoppi S AD - Istituto zooprofilattico sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10148, Torino, Italy. FAU - Dondo, Alessandro AU - Dondo A AD - Istituto zooprofilattico sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10148, Torino, Italy. FAU - Robetto, Serena AU - Robetto S AD - Istituto zooprofilattico sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10148, Torino, Italy. FAU - Rosati, Sergio AU - Rosati S AD - Department of Veterinary Science, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy. FAU - Lander, Angelika AU - Lander A AD - Robert Koch Institute, Seestraße 10, 13353, Berlin, Germany. FAU - Kurth, Andreas AU - Kurth A AD - Robert Koch Institute, Seestraße 10, 13353, Berlin, Germany. FAU - Orusa, Riccardo AU - Orusa R AD - Istituto zooprofilattico sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10148, Torino, Italy. FAU - Bertolotti, Luigi AU - Bertolotti L AD - Department of Veterinary Science, Largo Paolo Braccini 2, 10095, Grugliasco, TO, Italy. FAU - Mandola, Maria Lucia AU - Mandola ML AD - Istituto zooprofilattico sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10148, Torino, Italy. LA - eng GR - IZS PLV 09/13 RC/Ministero della Salute/ PT - Journal Article DEP - 20171222 PL - England TA - BMC Vet Res JT - BMC veterinary research JID - 101249759 SB - IM MH - Animals MH - Chiroptera/*virology MH - *Coronavirus/genetics MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Female MH - Italy/epidemiology MH - Male MH - *Paramyxoviridae/genetics MH - Paramyxoviridae Infections/epidemiology/*veterinary/virology MH - Phylogeny MH - Polymerase Chain Reaction/veterinary MH - Zoonoses/epidemiology/virology PMC - PMC5741894 OTO - NOTNLM OT - Bat-borne viruses OT - Coronavirus OT - Emerging viruses OT - Genetic characterization OT - Paramyxovirus OT - Surveillance COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Samples collection and bat species identification were performed by expert chiropterologists authorized by the Italian Ministry of Environment (authorization number DPN/2010/0011879 and 000882/PNM/08052014). All field operations were performed in compliance with the Habitats Directive of the European Union [41] and the Agreement on the Conservation of Populations of European Bats [45], and respecting ethical and safety recommendations [46]. CONSENT FOR PUBLICATION: Not applicable COMPETING INTERESTS: The authors declare no competing interests. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2017/12/24 06:00 MHDA- 2018/07/10 06:00 PMCR- 2017/12/22 CRDT- 2017/12/24 06:00 PHST- 2017/08/01 00:00 [received] PHST- 2017/11/28 00:00 [accepted] PHST- 2017/12/24 06:00 [entrez] PHST- 2017/12/24 06:00 [pubmed] PHST- 2018/07/10 06:00 [medline] PHST- 2017/12/22 00:00 [pmc-release] AID - 10.1186/s12917-017-1307-x [pii] AID - 1307 [pii] AID - 10.1186/s12917-017-1307-x [doi] PST - epublish SO - BMC Vet Res. 2017 Dec 22;13(1):396. doi: 10.1186/s12917-017-1307-x. PMID- 24871548 OWN - NLM STAT- MEDLINE DCOM- 20150601 LR - 20211021 IS - 1347-7439 (Electronic) IS - 0916-7250 (Print) IS - 0916-7250 (Linking) VI - 76 IP - 9 DP - 2014 Sep TI - Group B betacoronavirus in rhinolophid bats, Japan. PG - 1267-9 AB - We report group B Betacoronavirus infection in little Japanese horseshoe bats in Iwate prefecture. We then used reverse-transcription PCR to look for the coronavirus RNA-dependent RNA polymerase gene in fecal samples collected from 27 little Japanese horseshoe bats and found eight were provisionally positive. We had a success in the nucleotide sequencing of six of the eight positive samples and compared them with those of authentic coronaviruses. We found that these six samples were positive in coronavirus infection, and they belonged to the group B Betacornavirus by phylogenetic analysis. Virus isolation using the Vero cell culture was unsuccessful. Pathogenic trait of these bat coronaviruses remained unexplored. FAU - Suzuki, Jin AU - Suzuki J AD - Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan. FAU - Sato, Ryota AU - Sato R FAU - Kobayashi, Tomoya AU - Kobayashi T FAU - Aoi, Toshiki AU - Aoi T FAU - Harasawa, Ryô AU - Harasawa R LA - eng SI - GENBANK/AB889995 SI - GENBANK/AB889996 SI - GENBANK/AB889997 SI - GENBANK/AB889998 SI - GENBANK/AB889999 SI - GENBANK/AB890000 PT - Journal Article DEP - 20140527 PL - Japan TA - J Vet Med Sci JT - The Journal of veterinary medical science JID - 9105360 RN - 0 (RNA, Viral) RN - EC 2.7.7.49 (RNA-Directed DNA Polymerase) SB - IM MH - Animals MH - Base Sequence MH - Chiroptera/*virology MH - Coronavirus/enzymology/*genetics MH - Coronavirus Infections/epidemiology/*veterinary MH - Feces/enzymology/virology MH - Japan/epidemiology MH - Molecular Sequence Data MH - *Phylogeny MH - RNA, Viral/chemistry/genetics MH - RNA-Directed DNA Polymerase/*genetics MH - Reverse Transcriptase Polymerase Chain Reaction/veterinary MH - Sequence Alignment MH - Sequence Analysis, DNA PMC - PMC4197156 EDAT- 2014/05/30 06:00 MHDA- 2015/06/02 06:00 PMCR- 2014/09/01 CRDT- 2014/05/30 06:00 PHST- 2014/05/30 06:00 [entrez] PHST- 2014/05/30 06:00 [pubmed] PHST- 2015/06/02 06:00 [medline] PHST- 2014/09/01 00:00 [pmc-release] AID - DN/JST.JSTAGE/jvms/14-0012 [pii] AID - 14-0012 [pii] AID - 10.1292/jvms.14-0012 [doi] PST - ppublish SO - J Vet Med Sci. 2014 Sep;76(9):1267-9. doi: 10.1292/jvms.14-0012. Epub 2014 May 27. PMID- 26869154 OWN - NLM STAT- MEDLINE DCOM- 20180307 LR - 20180307 IS - 1544-2217 (Electronic) IS - 0300-9858 (Linking) VI - 53 IP - 3 DP - 2016 May TI - A Comparative Review of Animal Models of Middle East Respiratory Syndrome Coronavirus Infection. PG - 521-31 LID - 10.1177/0300985815620845 [doi] AB - Middle East respiratory syndrome coronavirus (MERS-CoV) was initially isolated from a Saudi Arabian man with fatal pneumonia. Since the original case in 2012, MERS-CoV infections have been reported in >1500 humans, and the case fatality rate is currently 35%. This lineage C betacoronavirus has been reported to cause a wide range of disease severity in humans, ranging from asymptomatic to progressive fatal pneumonia that may be accompanied by renal or multiorgan failure. Although the clinical presentation of human MERS-CoV infection has been documented, many facets of this emerging disease are still unknown and could be studied with animal models. Several animal models of MERS-CoV have been developed, including New Zealand white rabbits, transduced or transgenic mice that express human dipeptidyl peptidase 4, rhesus macaques, and common marmosets. This review provides an overview of the current state of knowledge on human MERS-CoV infections, the probable origin of MERS-CoV, and the available animal models of MERS-CoV infection. Evaluation of the benefits and limitations of these models will aid in appropriate model selection for studying viral pathogenesis and transmission, as well as for testing vaccines and antivirals against MERS-CoV. CI - © The Author(s) 2016. FAU - Baseler, L AU - Baseler L AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA Department of Veterinary Medicine and Surgery, University of Texas MD Anderson Cancer Center, Houston, TX, USA. FAU - de Wit, E AU - de Wit E AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA. FAU - Feldmann, H AU - Feldmann H AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA feldmannh@niaid.nih.gov. LA - eng PT - Journal Article PT - Review DEP - 20160211 PL - United States TA - Vet Pathol JT - Veterinary pathology JID - 0312020 RN - EC 3.4.14.5 (DPP4 protein, human) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Animals MH - Callithrix MH - *Coronavirus Infections/pathology/virology MH - Dipeptidyl Peptidase 4/genetics MH - *Disease Models, Animal MH - Humans MH - Macaca mulatta MH - Mice MH - Mice, Transgenic MH - Middle East Respiratory Syndrome Coronavirus/genetics/*pathogenicity MH - Rabbits OTO - NOTNLM OT - Middle East respiratory syndrome coronavirus OT - animal models OT - common marmosets OT - dipeptidyl peptidase 4 OT - mice OT - pathogenicity OT - rabbits OT - respiratory system OT - review OT - rhesus macaques OT - transgenic EDAT- 2016/02/13 06:00 MHDA- 2018/03/08 06:00 CRDT- 2016/02/13 06:00 PHST- 2016/02/13 06:00 [entrez] PHST- 2016/02/13 06:00 [pubmed] PHST- 2018/03/08 06:00 [medline] AID - 0300985815620845 [pii] AID - 10.1177/0300985815620845 [doi] PST - ppublish SO - Vet Pathol. 2016 May;53(3):521-31. doi: 10.1177/0300985815620845. Epub 2016 Feb 11. PMID- 25656066 OWN - NLM STAT- MEDLINE DCOM- 20160216 LR - 20231113 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 202 DP - 2015 Apr 16 TI - Middle East respiratory syndrome: An emerging coronavirus infection tracked by the crowd. PG - 60-88 LID - S0168-1702(15)00046-5 [pii] LID - 10.1016/j.virusres.2015.01.021 [doi] AB - In 2012 in Jordan, infection by a novel coronavirus (CoV) caused the first known cases of Middle East respiratory syndrome (MERS). MERS-CoV sequences have since been found in a bat and the virus appears to be enzootic among dromedary camels across the Arabian Peninsula and in parts of Africa. The majority of human cases have occurred in the Kingdom of Saudi Arabia (KSA). In humans, the etiologic agent, MERS-CoV, has been detected in severe, mild and influenza-like illness and in those without any obvious signs or symptoms of disease. MERS is often a lower respiratory tract disease associated with fever, cough, breathing difficulties, pneumonia that can progress to acute respiratory distress syndrome, multiorgan failure and death among more than a third of those infected. Severe disease is usually found in older males and comorbidities are frequently present in cases of MERS. Compared to SARS, MERS progresses more rapidly to respiratory failure and acute kidney injury, is more often observed as severe disease in patients with underlying illnesses and is more often fatal. MERS-CoV has a broader tropism than SARS-CoV, rapidly triggers cellular damage, employs a different receptor and induces a delayed proinflammatory response in cells. Most human cases have been linked to lapses in infection prevention and control in healthcare settings, with a fifth of virus detections reported among healthcare workers. This review sets out what is currently known about MERS and the MERS-CoV, summarises the new phenomenon of crowd-sourced epidemiology and lists some of the many questions that remain unanswered, nearly three years after the first reported case. CI - Copyright © 2015 Elsevier B.V. All rights reserved. FAU - Mackay, Ian M AU - Mackay IM AD - Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia. Electronic address: ian.mackay@uq.edu.au. FAU - Arden, Katherine E AU - Arden KE AD - Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, The University of Queensland, Brisbane, Australia. LA - eng PT - Journal Article PT - Review DEP - 20150202 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 SB - IM MH - Africa/epidemiology MH - Animals MH - Arabia/epidemiology MH - Camelus MH - Chiroptera MH - Communicable Diseases, Emerging/*epidemiology/pathology/virology MH - Coronavirus Infections/*epidemiology/pathology/virology MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/isolation & purification MH - Zoonoses/*epidemiology/pathology/virology PMC - PMC7114422 OTO - NOTNLM OT - Camel OT - Emerging infectious disease OT - Healthcare worker OT - MERS OT - MERS-CoV OT - Zoonosis EDAT- 2015/02/07 06:00 MHDA- 2016/02/18 06:00 PMCR- 2015/02/02 CRDT- 2015/02/07 06:00 PHST- 2014/06/09 00:00 [received] PHST- 2015/01/22 00:00 [revised] PHST- 2015/01/23 00:00 [accepted] PHST- 2015/02/07 06:00 [entrez] PHST- 2015/02/07 06:00 [pubmed] PHST- 2016/02/18 06:00 [medline] PHST- 2015/02/02 00:00 [pmc-release] AID - S0168-1702(15)00046-5 [pii] AID - 10.1016/j.virusres.2015.01.021 [doi] PST - ppublish SO - Virus Res. 2015 Apr 16;202:60-88. doi: 10.1016/j.virusres.2015.01.021. Epub 2015 Feb 2. PMID- 26788414 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20160120 LR - 20200930 IS - 2093-2340 (Print) IS - 2092-6448 (Electronic) IS - 1598-8112 (Linking) VI - 47 IP - 4 DP - 2015 Dec TI - Middle East Respiratory Syndrome Infection Control and Prevention Guideline for Healthcare Facilities. PG - 278-302 LID - 10.3947/ic.2015.47.4.278 [doi] AB - Middle East Respiratory Syndrome (MERS) is an acute viral respiratory illness with high mortality caused by a new strain of betacoronavirus (MERS-CoV). Since the report of the first patient in Saudi Arabia in 2012, large-scale outbreaks through hospital-acquired infection and inter-hospital transmission have been reported. Most of the patients reported in South Korea were also infected in hospital settings. Therefore, to eliminate the spread of MERS-CoV, infection prevention and control measures should be implemented with rigor. The present guideline has been drafted on the basis of the experiences of infection control in the South Korean hospitals involved in the recent MERS outbreak and on domestic and international infection prevention and control guidelines. To ensure efficient MERS-CoV infection prevention and control, care should be taken to provide comprehensive infection control measures including contact control, hand hygiene, personal protective equipment, disinfection, and environmental cleaning. FAU - Kim, Jin Yong AU - Kim JY AD - Division of Infectious Diseases, Department of Internal Medicine, Incheon Medical Center, Incheon, Korea. FAU - Song, Joon Young AU - Song JY AD - Division of Infectious Diseases, Department of Internal Medicine, Guro Hospital, Korea University College of Medicine, Seoul, Korea. FAU - Yoon, Young Kyung AU - Yoon YK AD - Division of Infectious Diseases, Department of Internal Medicine, Anam Hospital, Korea University College of Medicine, Seoul, Korea. FAU - Choi, Seong-Ho AU - Choi SH AD - Division of Infectious Diseases, Department of Internal Medicine, Seoul Hospital, Chung-Ang University College of Medicine, Seoul, Korea. FAU - Song, Young Goo AU - Song YG AD - Division of Infectious Diseases, Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. FAU - Kim, Sung-Ran AU - Kim SR AD - Department of Infection Control, Korea University Guro Hospital, Seoul, Korea. FAU - Son, Hee-Jung AU - Son HJ AD - Department of Infection Control, Ewha Womens University Mokdong Hospital, Seoul, Korea. FAU - Jeong, Sun-Young AU - Jeong SY AD - Keonyang University College of Nursing, Daejeon, Korea. FAU - Choi, Jung-Hwa AU - Choi JH AD - Department of Infection Control, Konkuk University Seoul Hospital, Seoul, Korea. FAU - Kim, Kyung Mi AU - Kim KM AD - Catholic University of Korea College of Nursing, Seoul, Korea. FAU - Yoon, Hee Jung AU - Yoon HJ AD - Division of Infectious Diseases, Department of Internal Medicine, Seoul Metropolitan Government Seobuk Hospital, Seoul, Korea. FAU - Choi, Jun Yong AU - Choi JY AD - Division of Infectious Diseases, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. FAU - Kim, Tae Hyong AU - Kim TH AD - Division of Infectious Diseases, Department of Internal Medicine, Soonchunhyang University College of Medicine, Seoul, Korea. FAU - Choi, Young Hwa AU - Choi YH AD - Division of Infectious Diseases, Department of Internal Medicine, Ajou University School of Medicine, Suwon, Korea. FAU - Kim, Hong Bin AU - Kim HB AD - Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea. FAU - Yoon, Ji Hyun AU - Yoon JH AD - Division of Infectious Diseases, Department of Internal Medicine, Eulji University Hospital, Daejeon, Korea. FAU - Lee, Jacob AU - Lee J AD - Division of Infectious Diseases, Department of Internal Medicine, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea. FAU - Eom, Joong Sik AU - Eom JS AD - Division of Infectious Diseases, Department of Internal Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea. FAU - Lee, Sang-Oh AU - Lee SO AD - Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. FAU - Oh, Won Sup AU - Oh WS AD - Division of Infectious Diseases, Department of Internal Medicine, Kangwon National University School of Medicine, Chucheon, Korea. FAU - Choi, Jung-Hyun AU - Choi JH AD - Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. FAU - Yoo, Jin-Hong AU - Yoo JH AD - Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. FAU - Kim, Woo Joo AU - Kim WJ AD - Division of Infectious Diseases, Department of Internal Medicine, Guro Hospital, Korea University College of Medicine, Seoul, Korea. FAU - Cheong, Hee Jin AU - Cheong HJ AD - Division of Infectious Diseases, Department of Internal Medicine, Guro Hospital, Korea University College of Medicine, Seoul, Korea. LA - eng PT - Journal Article PT - Review DEP - 20151230 PL - Korea (South) TA - Infect Chemother JT - Infection & chemotherapy JID - 101531537 PMC - PMC4716282 OTO - NOTNLM OT - Disinfection OT - Infection control OT - Middle east respiratory syndrome coronavirus OT - Personal protective equipment OT - Quarantine COIS- Conflict of Interest: This guideline was developed with the support of the Korean Society of Infectious Diseases, Korean Society for Healthcare-associated Infection Control and Prevention, and Korean Association of Infection Control Nurses. We hereby declare that the researchers participating in the development and review of this guideline did not receive any research grant that could potentially influence the finalized guideline and that they were not influenced by any particular interest groups. EDAT- 2016/01/21 06:00 MHDA- 2016/01/21 06:01 PMCR- 2015/12/01 CRDT- 2016/01/21 06:00 PHST- 2015/12/01 00:00 [received] PHST- 2016/01/21 06:00 [entrez] PHST- 2016/01/21 06:00 [pubmed] PHST- 2016/01/21 06:01 [medline] PHST- 2015/12/01 00:00 [pmc-release] AID - 10.3947/ic.2015.47.4.278 [doi] PST - ppublish SO - Infect Chemother. 2015 Dec;47(4):278-302. doi: 10.3947/ic.2015.47.4.278. Epub 2015 Dec 30. PMID- 29223446 OWN - NLM STAT- MEDLINE DCOM- 20180420 LR - 20210109 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 517 DP - 2018 Apr TI - Structural and functional conservation of cis-acting RNA elements in coronavirus 5'-terminal genome regions. PG - 44-55 LID - S0042-6822(17)30404-X [pii] LID - 10.1016/j.virol.2017.11.025 [doi] AB - Structure predictions suggest a partial conservation of RNA structure elements in coronavirus terminal genome regions. Here, we determined the structures of stem-loops (SL) 1 and 2 of two alphacoronaviruses, human coronavirus (HCoV) 229E and NL63, by RNA structure probing and studied the functional relevance of these putative cis-acting elements. HCoV-229E SL1 and SL2 mutants generated by reverse genetics were used to study the effects on viral replication of single-nucleotide substitutions predicted to destabilize the SL1 and SL2 structures. The data provide conclusive evidence for the critical role of SL1 and SL2 in HCoV-229E replication and, in some cases, revealed parallels with previously characterized betacoronavirus SL1 and SL2 elements. Also, we were able to rescue viable HCoV-229E mutants carrying replacements of SL2 with equivalent betacoronavirus structural elements. The data obtained in this study reveal a remarkable degree of structural and functional conservation of 5'-terminal RNA structural elements across coronavirus genus boundaries. CI - Copyright © 2017 Elsevier Inc. All rights reserved. FAU - Madhugiri, Ramakanth AU - Madhugiri R AD - Institute of Medical Virology, Justus Liebig University, Giessen, Germany. FAU - Karl, Nadja AU - Karl N AD - Institute of Medical Virology, Justus Liebig University, Giessen, Germany. FAU - Petersen, Daniel AU - Petersen D AD - Institute of Medical Virology, Justus Liebig University, Giessen, Germany. FAU - Lamkiewicz, Kevin AU - Lamkiewicz K AD - Faculty of Mathematics and Computer Science, Friedrich Schiller University, Jena, Germany; European Virus Bioinformatics Center, Jena, Germany. FAU - Fricke, Markus AU - Fricke M AD - Faculty of Mathematics and Computer Science, Friedrich Schiller University, Jena, Germany; European Virus Bioinformatics Center, Jena, Germany. FAU - Wend, Ulrike AU - Wend U AD - Institute of Medical Virology, Justus Liebig University, Giessen, Germany. FAU - Scheuer, Robina AU - Scheuer R AD - Institute of Medical Virology, Justus Liebig University, Giessen, Germany. FAU - Marz, Manja AU - Marz M AD - Faculty of Mathematics and Computer Science, Friedrich Schiller University, Jena, Germany; FLI Leibniz Institute for Age Research, Jena, Germany; European Virus Bioinformatics Center, Jena, Germany. FAU - Ziebuhr, John AU - Ziebuhr J AD - Institute of Medical Virology, Justus Liebig University, Giessen, Germany; European Virus Bioinformatics Center, Jena, Germany. Electronic address: john.ziebuhr@viro.med.uni-giessen.de. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20171206 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (RNA, Viral) SB - IM MH - Base Sequence MH - Cell Line MH - Coronavirus 229E, Human/*genetics MH - Coronavirus NL63, Human/*genetics MH - *Genome, Viral MH - Humans MH - Nucleic Acid Conformation MH - RNA, Viral/chemistry/genetics MH - Regulatory Sequences, Nucleic Acid/*physiology MH - Virus Replication/physiology PMC - PMC7112051 OTO - NOTNLM OT - Coronavirus OT - Coronavirus phylogeny OT - RNA structure OT - Replication OT - Stem-loop OT - cis-acting RNA element EDAT- 2017/12/11 06:00 MHDA- 2018/04/21 06:00 PMCR- 2017/12/06 CRDT- 2017/12/11 06:00 PHST- 2017/10/01 00:00 [received] PHST- 2017/11/28 00:00 [revised] PHST- 2017/11/29 00:00 [accepted] PHST- 2017/12/11 06:00 [pubmed] PHST- 2018/04/21 06:00 [medline] PHST- 2017/12/11 06:00 [entrez] PHST- 2017/12/06 00:00 [pmc-release] AID - S0042-6822(17)30404-X [pii] AID - 10.1016/j.virol.2017.11.025 [doi] PST - ppublish SO - Virology. 2018 Apr;517:44-55. doi: 10.1016/j.virol.2017.11.025. Epub 2017 Dec 6. PMID- 29760102 OWN - NLM STAT- MEDLINE DCOM- 20180904 LR - 20200306 IS - 1091-6490 (Electronic) IS - 0027-8424 (Print) IS - 0027-8424 (Linking) VI - 115 IP - 22 DP - 2018 May 29 TI - Broad receptor engagement of an emerging global coronavirus may potentiate its diverse cross-species transmissibility. PG - E5135-E5143 LID - 10.1073/pnas.1802879115 [doi] AB - Porcine deltacoronavirus (PDCoV), identified in 2012, is a common enteropathogen of swine with worldwide distribution. The source and evolutionary history of this virus is, however, unknown. PDCoV belongs to the Deltacoronavirus genus that comprises predominantly avian CoV. Phylogenetic analysis suggests that PDCoV originated relatively recently from a host-switching event between birds and mammals. Insight into receptor engagement by PDCoV may shed light into such an exceptional phenomenon. Here we report that PDCoV employs host aminopeptidase N (APN) as an entry receptor and interacts with APN via domain B of its spike (S) protein. Infection of porcine cells with PDCoV was drastically reduced by APN knockout and rescued after reconstitution of APN expression. In addition, we observed that PDCoV efficiently infects cells of unusual broad species range, including human and chicken. Accordingly, PDCoV S was found to target the phylogenetically conserved catalytic domain of APN. Moreover, transient expression of porcine, feline, human, and chicken APN renders cells susceptible to PDCoV infection. Binding of PDCoV to an interspecies conserved site on APN may facilitate direct transmission of PDCoV to nonreservoir species, including humans, potentially reflecting the mechanism that enabled a virus, ancestral to PDCoV, to breach the species barrier between birds and mammals. The APN cell surface protein is also used by several members of the Alphacoronavirus genus. Hence, our data constitute the second identification of CoVs from different genera that use the same receptor, implying that CoV receptor selection is subjected to specific restrictions that are still poorly understood. FAU - Li, Wentao AU - Li W AD - Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands. FAU - Hulswit, Ruben J G AU - Hulswit RJG AD - Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands. FAU - Kenney, Scott P AU - Kenney SP AD - Department of Veterinary Preventive Medicine, Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691. FAU - Widjaja, Ivy AU - Widjaja I AD - Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands. FAU - Jung, Kwonil AU - Jung K AD - Department of Veterinary Preventive Medicine, Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691. FAU - Alhamo, Moyasar A AU - Alhamo MA AD - Department of Veterinary Preventive Medicine, Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691. FAU - van Dieren, Brenda AU - van Dieren B AD - Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands. FAU - van Kuppeveld, Frank J M AU - van Kuppeveld FJM AD - Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands. FAU - Saif, Linda J AU - Saif LJ AD - Department of Veterinary Preventive Medicine, Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691 saif.2@osu.edu b.j.bosch@uu.nl. FAU - Bosch, Berend-Jan AU - Bosch BJ AD - Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands; saif.2@osu.edu b.j.bosch@uu.nl. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180514 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Antibodies, Viral) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.11.2 (CD13 Antigens) SB - IM MH - Animals MH - Antibodies, Viral/immunology MH - CD13 Antigens/metabolism MH - Cats MH - Cell Line MH - Chickens MH - Chlorocebus aethiops MH - *Communicable Diseases, Emerging/transmission/veterinary/virology MH - Coronavirus/immunology/*pathogenicity/*physiology MH - *Coronavirus Infections/transmission/veterinary/virology MH - Dogs MH - Host Specificity MH - Host-Pathogen Interactions/*physiology MH - Humans MH - Madin Darby Canine Kidney Cells MH - Mice MH - Spike Glycoprotein, Coronavirus/chemistry/genetics/metabolism MH - Swine MH - Vero Cells MH - Zoonoses/transmission/virology PMC - PMC5984533 OTO - NOTNLM OT - APN OT - PDCoV OT - cross-species transmission OT - receptor OT - spike COIS- The authors declare no conflict of interest. EDAT- 2018/05/16 06:00 MHDA- 2018/09/05 06:00 PMCR- 2018/05/14 CRDT- 2018/05/16 06:00 PHST- 2018/05/16 06:00 [pubmed] PHST- 2018/09/05 06:00 [medline] PHST- 2018/05/16 06:00 [entrez] PHST- 2018/05/14 00:00 [pmc-release] AID - 1802879115 [pii] AID - 201802879 [pii] AID - 10.1073/pnas.1802879115 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5135-E5143. doi: 10.1073/pnas.1802879115. Epub 2018 May 14. PMID- 30704076 OWN - NLM STAT- MEDLINE DCOM- 20190619 LR - 20240715 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 11 IP - 2 DP - 2019 Jan 30 TI - Shared Common Ancestry of Rodent Alphacoronaviruses Sampled Globally. LID - 10.3390/v11020125 [doi] LID - 125 AB - The recent discovery of novel alphacoronaviruses (alpha-CoVs) in European and Asian rodents revealed that rodent coronaviruses (CoVs) sampled worldwide formed a discrete phylogenetic group within this genus. To determine the evolutionary history of rodent CoVs in more detail, particularly the relative frequencies of virus-host co-divergence and cross-species transmission, we recovered longer fragments of CoV genomes from previously discovered European rodent alpha-CoVs using a combination of PCR and high-throughput sequencing. Accordingly, the full genome sequence was retrieved from the UK rat coronavirus, along with partial genome sequences from the UK field vole and Poland-resident bank vole CoVs, and a short conserved ORF1b fragment from the French rabbit CoV. Genome and phylogenetic analysis showed that despite their diverse geographic origins, all rodent alpha-CoVs formed a single monophyletic group and shared similar features, such as the same gene constellations, a recombinant beta-CoV spike gene, and similar core transcriptional regulatory sequences (TRS). These data suggest that all rodent alpha CoVs sampled so far originate from a single common ancestor, and that there has likely been a long-term association between alpha CoVs and rodents. Despite this likely antiquity, the phylogenetic pattern of the alpha-CoVs was also suggestive of relatively frequent host-jumping among the different rodent species. FAU - Tsoleridis, Theocharis AU - Tsoleridis T AUID- ORCID: 0000-0002-9685-6318 AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. t.tsoleridis@nottingham.ac.uk. FAU - Chappell, Joseph G AU - Chappell JG AUID- ORCID: 0000-0002-8205-4242 AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. nixjc1@exmail.nottingham.ac.uk. FAU - Onianwa, Okechukwu AU - Onianwa O AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. okechukwu.onianwa@phe.gov.uk. FAU - Marston, Denise A AU - Marston DA AD - Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency (APHA), Weybridge-London KT15 3NB, UK. Denise.Marston@apha.gsi.gov.uk. FAU - Fooks, Anthony R AU - Fooks AR AUID- ORCID: 0000-0002-3243-6154 AD - Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency (APHA), Weybridge-London KT15 3NB, UK. Tony.Fooks@apha.gsi.gov.uk. FAU - Monchatre-Leroy, Elodie AU - Monchatre-Leroy E AUID- ORCID: 0000-0002-3231-398X AD - Anses, Laboratoire de la rage et de la faune sauvage, 54220 Malzéville, France. elodie.monchatre-leroy@anses.fr. FAU - Umhang, Gérald AU - Umhang G AD - Anses, Laboratoire de la rage et de la faune sauvage, 54220 Malzéville, France. gerald.umhang@anses.fr. FAU - Müller, Marcel A AU - Müller MA AD - Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, 10117 Berlin, Germany. marcel.mueller@charite.de. FAU - Drexler, Jan F AU - Drexler JF AD - Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, 10117 Berlin, Germany. felix.drexler@charite.de. FAU - Drosten, Christian AU - Drosten C AD - Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, 10117 Berlin, Germany. christian.drosten@charite.de. FAU - Tarlinton, Rachael E AU - Tarlinton RE AUID- ORCID: 0000-0003-3325-2311 AD - School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK. rachael.tarlinton@nottingham.ac.uk. FAU - McClure, Charles P AU - McClure CP AUID- ORCID: 0000-0002-1710-1049 AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. patrick.mcclure@nottingham.ac.uk. FAU - Holmes, Edward C AU - Holmes EC AUID- ORCID: 0000-0001-9596-3552 AD - Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia. edward.holmes@sydney.edu.au. FAU - Ball, Jonathan K AU - Ball JK AD - School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK. jonathan.ball@nottingham.ac.uk. LA - eng GR - BB/J014508/1/Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190130 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Alphacoronavirus/*classification MH - Animals MH - Arvicolinae/virology MH - Asia MH - Coronavirus/genetics MH - Coronavirus Infections/transmission MH - Europe MH - *Evolution, Molecular MH - Genetic Variation MH - *Genome, Viral MH - Murinae/virology MH - Phylogeny MH - Rabbits/virology MH - Rats/virology MH - Recombination, Genetic MH - Rodentia/*virology MH - Sequence Analysis, DNA PMC - PMC6409636 OTO - NOTNLM OT - alphacoronavirus OT - ancestry OT - coronavirus OT - evolution OT - recombination OT - rodents COIS- The authors declare no conflict of interest. EDAT- 2019/02/02 06:00 MHDA- 2019/06/20 06:00 PMCR- 2019/02/01 CRDT- 2019/02/02 06:00 PHST- 2019/01/14 00:00 [received] PHST- 2019/01/25 00:00 [revised] PHST- 2019/01/28 00:00 [accepted] PHST- 2019/02/02 06:00 [entrez] PHST- 2019/02/02 06:00 [pubmed] PHST- 2019/06/20 06:00 [medline] PHST- 2019/02/01 00:00 [pmc-release] AID - v11020125 [pii] AID - viruses-11-00125 [pii] AID - 10.3390/v11020125 [doi] PST - epublish SO - Viruses. 2019 Jan 30;11(2):125. doi: 10.3390/v11020125. PMID- 27178103 OWN - NLM STAT- MEDLINE DCOM- 20170913 LR - 20221207 IS - 1863-2378 (Electronic) IS - 1863-1959 (Print) IS - 1863-1959 (Linking) VI - 63 IP - 8 DP - 2016 Dec TI - Detection of the Severe Acute Respiratory Syndrome-Related Coronavirus and Alphacoronavirus in the Bat Population of Taiwan. PG - 608-615 LID - 10.1111/zph.12271 [doi] AB - Bats have been demonstrated to be natural reservoirs of severe acute respiratory syndrome coronavirus (SARS CoV) and Middle East respiratory syndrome (MERS) CoV. Faecal samples from 248 individuals of 20 bat species were tested for partial RNA-dependent RNA polymerase gene of CoV and 57 faecal samples from eight bat species were tested positive. The highest detection rate of 44% for Scotophilus kuhlii, followed by 30% for Rhinolophus monoceros. Significantly higher detection rates of coronaviral RNA were found in female bats and Scotophilus kuhlii roosting in palm trees. Phylogenetic analysis classified the positive samples into SARS-related (SARSr) CoV, Scotophilus bat CoV 512 close to those from China and Philippines, and Miniopterus bat CoV 1A-related lineages. Coronaviral RNA was also detected in bat guano from Scotophilus kuhlii and Myotis formosus flavus on the ground and had potential risk for human exposure. Diverse bat CoV with zoonotic potential could be introduced by migratory bats and maintained in the endemic bat population in Taiwan. CI - © 2016 Blackwell Verlag GmbH. FAU - Chen, Y-N AU - Chen YN AD - Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan, Taiwan. FAU - Phuong, V N AU - Phuong VN AD - Department of Bioscience Technology, Chung Yuan Christian University, Taoyuan, Taiwan. FAU - Chen, H C AU - Chen HC AD - Institute of Ecology and Evolutionary Biology, National Taiwan University, Taipei, Taiwan. FAU - Chou, C-H AU - Chou CH AD - Endemic Species Research Institute, Council of Agriculture, Nantou, Taiwan. FAU - Cheng, H-C AU - Cheng HC AD - Endemic Species Research Institute, Council of Agriculture, Nantou, Taiwan. FAU - Wu, C-H AU - Wu CH AD - Department of Applied Mathematics, Chung Yuan Christian University, Taoyuan, Taiwan. LA - eng SI - GENBANK/KT381902 SI - GENBANK/KT381925 PT - Journal Article DEP - 20160513 PL - Germany TA - Zoonoses Public Health JT - Zoonoses and public health JID - 101300786 RN - 0 (RNA, Viral) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Animals MH - Chiroptera/*virology MH - Feces/virology MH - Female MH - Male MH - Phylogeny MH - RNA, Viral/isolation & purification MH - RNA-Dependent RNA Polymerase/genetics MH - Risk Factors MH - Severe acute respiratory syndrome-related coronavirus/classification/genetics/*isolation & purification MH - Severe Acute Respiratory Syndrome/epidemiology/*veterinary MH - Species Specificity MH - Taiwan MH - Zoonoses PMC - PMC7165716 OTO - NOTNLM OT - Chiroptera OT - Taiwan OT - coronavirus OT - reverse transcription polymerase chain reaction OT - severe acute respiratory syndrome virus OT - zoonosis EDAT- 2016/05/15 06:00 MHDA- 2017/09/14 06:00 PMCR- 2020/04/18 CRDT- 2016/05/15 06:00 PHST- 2015/12/02 00:00 [received] PHST- 2016/05/15 06:00 [pubmed] PHST- 2017/09/14 06:00 [medline] PHST- 2016/05/15 06:00 [entrez] PHST- 2020/04/18 00:00 [pmc-release] AID - ZPH12271 [pii] AID - 10.1111/zph.12271 [doi] PST - ppublish SO - Zoonoses Public Health. 2016 Dec;63(8):608-615. doi: 10.1111/zph.12271. Epub 2016 May 13. PMID- 27125516 OWN - NLM STAT- MEDLINE DCOM- 20170317 LR - 20200325 IS - 1869-1889 (Electronic) IS - 1674-7305 (Print) IS - 1674-7305 (Linking) VI - 59 IP - 6 DP - 2016 Jun TI - Genetic diversity of coronaviruses in Miniopterus fuliginosus bats. PG - 604-14 LID - 10.1007/s11427-016-5039-0 [doi] AB - Coronaviruses, such as severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, pose significant public health threats. Bats have been suggested to act as natural reservoirs for both these viruses, and periodic monitoring of coronaviruses in bats may thus provide important clues about emergent infectious viruses. The Eastern bent-wing bat Miniopterus fuliginosus is distributed extensively throughout China. We therefore analyzed the genetic diversity of coronaviruses in samples of M. fuliginosus collected from nine Chinese provinces during 2011-2013. The only coronavirus genus found was Alphacoronavirus. We established six complete and five partial genomic sequences of alphacoronaviruses, which revealed that they could be divided into two distinct lineages, with close relationships to coronaviruses in Miniopterus magnater and Miniopterus pusillus. Recombination was confirmed by detecting putative breakpoints of Lineage 1 coronaviruses in M. fuliginosus and M. pusillus (Wu et al., 2015), which supported the results of topological and phylogenetic analyses. The established alphacoronavirus genome sequences showed high similarity to other alphacoronaviruses found in other Miniopterus species, suggesting that their transmission in different Miniopterus species may provide opportunities for recombination with different alphacoronaviruses. The genetic information for these novel alphacoronaviruses will improve our understanding of the evolution and genetic diversity of coronaviruses, with potentially important implications for the transmission of human diseases. FAU - Du, Jiang AU - Du J AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. FAU - Yang, Li AU - Yang L AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. FAU - Ren, Xianwen AU - Ren X AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. FAU - Zhang, Junpeng AU - Zhang J AD - State Key Laboratory of Estuarine and Coastal Research, Institute of Estuarine and Coastal Research, East China Normal University, Shanghai, 200062, China. FAU - Dong, Jie AU - Dong J AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. FAU - Sun, Lilian AU - Sun L AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. FAU - Zhu, Yafang AU - Zhu Y AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. FAU - Yang, Fan AU - Yang F AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. FAU - Zhang, Shuyi AU - Zhang S AD - College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. FAU - Wu, Zhiqiang AU - Wu Z AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. wuzq2009@ipbcams.ac.cn. FAU - Jin, Qi AU - Jin Q AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100176, China. zdsys@vip.sina.com. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, 310003, China. zdsys@vip.sina.com. LA - eng PT - Journal Article DEP - 20160428 PL - China TA - Sci China Life Sci JT - Science China. Life sciences JID - 101529880 SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronavirus/classification/*genetics MH - Genes, Viral MH - *Genetic Variation MH - Phylogeny PMC - PMC7089092 OTO - NOTNLM OT - Miniopterus fuliginosus OT - bat OT - co-infection OT - coronavirus OT - recombination EDAT- 2016/04/30 06:00 MHDA- 2017/03/18 06:00 PMCR- 2016/04/28 CRDT- 2016/04/30 06:00 PHST- 2016/02/01 00:00 [received] PHST- 2016/02/22 00:00 [accepted] PHST- 2016/04/30 06:00 [entrez] PHST- 2016/04/30 06:00 [pubmed] PHST- 2017/03/18 06:00 [medline] PHST- 2016/04/28 00:00 [pmc-release] AID - 10.1007/s11427-016-5039-0 [pii] AID - 5039 [pii] AID - 10.1007/s11427-016-5039-0 [doi] PST - ppublish SO - Sci China Life Sci. 2016 Jun;59(6):604-14. doi: 10.1007/s11427-016-5039-0. Epub 2016 Apr 28. PMID- 28580734 OWN - NLM STAT- MEDLINE DCOM- 20170925 LR - 20230807 IS - 1469-896X (Electronic) IS - 0961-8368 (Print) IS - 0961-8368 (Linking) VI - 26 IP - 9 DP - 2017 Sep TI - SARS-unique fold in the Rousettus bat coronavirus HKU9. PG - 1726-1737 LID - 10.1002/pro.3208 [doi] AB - The coronavirus nonstructural protein 3 (nsp3) is a multifunctional protein that comprises multiple structural domains. This protein assists viral polyprotein cleavage, host immune interference, and may play other roles in genome replication or transcription. Here, we report the solution NMR structure of a protein from the "SARS-unique region" of the bat coronavirus HKU9. The protein contains a frataxin fold or double-wing motif, which is an α + β fold that is associated with protein/protein interactions, DNA binding, and metal ion binding. High structural similarity to the human severe acute respiratory syndrome (SARS) coronavirus nsp3 is present. A possible functional site that is conserved among some betacoronaviruses has been identified using bioinformatics and biochemical analyses. This structure provides strong experimental support for the recent proposal advanced by us and others that the "SARS-unique" region is not unique to the human SARS virus, but is conserved among several different phylogenetic groups of coronaviruses and provides essential functions. CI - © 2017 The Protein Society. FAU - Hammond, Robert G AU - Hammond RG AD - Department of Chemistry, University of Alabama at Birmingham, Birmingham, Alabama, 35294. FAU - Tan, Xuan AU - Tan X AD - Department of Chemistry, University of Alabama at Birmingham, Birmingham, Alabama, 35294. FAU - Johnson, Margaret A AU - Johnson MA AUID- ORCID: 0000-0002-4422-399X AD - Department of Chemistry, University of Alabama at Birmingham, Birmingham, Alabama, 35294. LA - eng GR - R35 GM119456/GM/NIGMS NIH HHS/United States GR - S10 RR022994/RR/NCRR NIH HHS/United States PT - Journal Article DEP - 20170615 PL - United States TA - Protein Sci JT - Protein science : a publication of the Protein Society JID - 9211750 RN - 0 (Viral Nonstructural Proteins) RN - EC 2.7.7.48 (Nsp3 protein, SARS-CoV) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Animals MH - Chiroptera MH - Coronavirus/*chemistry MH - Models, Molecular MH - Nuclear Magnetic Resonance, Biomolecular MH - Protein Domains MH - Protein Folding MH - RNA-Dependent RNA Polymerase/*chemistry/*metabolism MH - Severe acute respiratory syndrome-related coronavirus/*chemistry MH - Viral Nonstructural Proteins/*chemistry/*metabolism PMC - PMC5563143 OTO - NOTNLM OT - NMR OT - SARS-unique domain OT - coronavirus OT - double-wing motif OT - frataxin OT - nonstructural protein OT - protein functional annotation OT - viral protein EDAT- 2017/06/06 06:00 MHDA- 2017/09/26 06:00 PMCR- 2018/09/01 CRDT- 2017/06/06 06:00 PHST- 2017/03/01 00:00 [received] PHST- 2017/05/26 00:00 [revised] PHST- 2017/05/26 00:00 [accepted] PHST- 2017/06/06 06:00 [pubmed] PHST- 2017/09/26 06:00 [medline] PHST- 2017/06/06 06:00 [entrez] PHST- 2018/09/01 00:00 [pmc-release] AID - PRO3208 [pii] AID - 10.1002/pro.3208 [doi] PST - ppublish SO - Protein Sci. 2017 Sep;26(9):1726-1737. doi: 10.1002/pro.3208. Epub 2017 Jun 15. PMID- 30258004 OWN - NLM STAT- MEDLINE DCOM- 20190830 LR - 20221207 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 24 DP - 2018 Dec 15 TI - Lysosomal Proteases Are a Determinant of Coronavirus Tropism. LID - 10.1128/JVI.01504-18 [doi] LID - e01504-18 AB - Cell entry by coronaviruses involves two principal steps, receptor binding and membrane fusion; the latter requires activation by host proteases, particularly lysosomal proteases. Despite the importance of lysosomal proteases in both coronavirus entry and cell metabolism, the correlation between lysosomal proteases and cell tropism of coronaviruses has not been established. Here, we examined the roles of lysosomal proteases in activating coronavirus surface spike proteins for membrane fusion, using the spike proteins from severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) as the model system. To this end, we controlled the contributions from receptor binding and other host proteases, thereby attributing coronavirus entry solely or mainly to the efficiency of lysosomal proteases in activating coronavirus spike-mediated membrane fusion. Our results showed that lysosomal proteases from bat cells support coronavirus spike-mediated pseudovirus entry and cell-cell fusion more effectively than their counterparts from human cells. Moreover, purified lysosomal extracts from bat cells cleave cell surface-expressed coronavirus spikes more efficiently than their counterparts from human cells. Overall, our study suggests that different lysosomal protease activities from different host species and tissue cells are an important determinant of the species and tissue tropism of coronaviruses.IMPORTANCE Coronaviruses are capable of colonizing new species, as evidenced by the recent emergence of SARS and MERS coronaviruses; they can also infect multiple tissues in the same species. Lysosomal proteases play critical roles in coronavirus entry by cleaving coronavirus surface spike proteins and activating the fusion of host and viral membranes; they also play critical roles in cell physiology by processing cellular products. How do different lysosomal protease activities from different cells impact coronavirus entry? Here, we controlled the contributions from known factors that function in coronavirus entry so that lysosomal protease activities became the only or the main determinant of coronavirus entry. Using pseudovirus entry, cell-cell fusion, and biochemical assays, we showed that lysosomal proteases from bat cells activate coronavirus spike-mediated membrane fusion more efficiently than their counterparts from human cells. Our study provides the first direct evidence supporting lysosomal proteases as a determinant of the species and tissue tropisms of coronaviruses. CI - Copyright © 2018 American Society for Microbiology. FAU - Zheng, Yuan AU - Zheng Y AD - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Shang, Jian AU - Shang J AD - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Yang, Yang AU - Yang Y AD - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Liu, Chang AU - Liu C AD - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Wan, Yushun AU - Wan Y AD - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Geng, Qibin AU - Geng Q AD - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Wang, Michelle AU - Wang M AD - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Baric, Ralph AU - Baric R AD - Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina, USA. FAU - Li, Fang AU - Li F AD - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota, USA lifang@umn.edu. LA - eng GR - R01 AI089728/AI/NIAID NIH HHS/United States GR - R01 AI110700/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20181127 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.- (Peptide Hydrolases) SB - IM MH - A549 Cells MH - Animals MH - Cells, Cultured MH - Chiroptera MH - Chlorocebus aethiops MH - Coronavirus Infections/*metabolism MH - HEK293 Cells MH - HeLa Cells MH - Humans MH - Lysosomes/*enzymology MH - Middle East Respiratory Syndrome Coronavirus/metabolism/*physiology MH - Peptide Hydrolases/*metabolism MH - Severe acute respiratory syndrome-related coronavirus/metabolism/*physiology MH - Spike Glycoprotein, Coronavirus/*metabolism MH - Vero Cells MH - Viral Tropism MH - Virus Internalization PMC - PMC6258935 OTO - NOTNLM OT - coronavirus spike protein OT - lysosomal proteases OT - species tropism OT - tissue tropism EDAT- 2018/09/28 06:00 MHDA- 2019/08/31 06:00 PMCR- 2019/05/27 CRDT- 2018/09/28 06:00 PHST- 2018/08/30 00:00 [received] PHST- 2018/09/19 00:00 [accepted] PHST- 2018/09/28 06:00 [pubmed] PHST- 2019/08/31 06:00 [medline] PHST- 2018/09/28 06:00 [entrez] PHST- 2019/05/27 00:00 [pmc-release] AID - JVI.01504-18 [pii] AID - JVI01504-18 [pii] AID - 10.1128/JVI.01504-18 [doi] PST - epublish SO - J Virol. 2018 Nov 27;92(24):e01504-18. doi: 10.1128/JVI.01504-18. Print 2018 Dec 15. PMID- 28296228 OWN - NLM STAT- MEDLINE DCOM- 20180511 LR - 20200423 IS - 1865-1682 (Electronic) IS - 1865-1674 (Print) IS - 1865-1674 (Linking) VI - 64 IP - 6 DP - 2017 Dec TI - Coronavirus infections in horses in Saudi Arabia and Oman. PG - 2093-2103 LID - 10.1111/tbed.12630 [doi] AB - Equine coronaviruses (ECoV) are the only coronavirus known to infect horses. So far, data on ECoV infection in horses remain limited to the USA, France and Japan and its geographic distribution is not well understood. We carried out RT-PCR on 306 nasal and 315 rectal swabs and tested 243 sera for antibodies to detect coronavirus infections in apparently healthy horses in Saudi Arabia and Oman. We document evidence of infection with ECoV and HKU23 coronavirus by RT-PCR. There was no conclusive evidence of Middle East respiratory syndrome coronavirus infection in horses. Serological data suggest that lineage A betacoronavirus infections are commonly infecting horses in Saudi Arabia and Oman but antibody cross-reactivities between these viruses do not permit us to use serological data alone to identify which coronaviruses are causing these infections. CI - © 2017 Blackwell Verlag GmbH. FAU - Hemida, M G AU - Hemida MG AD - Department of Microbiology and Parasitology, College of Veterinary Medicine, King Faisal University, Al-Hasa, Saudi Arabia. AD - Department of Virology Faculty of Veterinary Medicine, Kaferelsheik University, Kaferelsheik, Egypt. FAU - Chu, D K W AU - Chu DKW AUID- ORCID: 0000-0002-9219-8979 AD - School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Perera, R A P M AU - Perera RAPM AD - School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Ko, R L W AU - Ko RLW AD - School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - So, R T Y AU - So RTY AD - School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Ng, B C Y AU - Ng BCY AD - School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Chan, S M S AU - Chan SMS AD - School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Chu, S AU - Chu S AD - School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Alnaeem, A A AU - Alnaeem AA AD - Department of Clinical Studies, College of Veterinary Medicine, King Faisal University, Al-Hasa, Saudi Arabia. FAU - Alhammadi, M A AU - Alhammadi MA AD - Department of Microbiology and Parasitology, College of Veterinary Medicine, King Faisal University, Al-Hasa, Saudi Arabia. FAU - Webby, R J AU - Webby RJ AD - Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA. FAU - Poon, L L M AU - Poon LLM AD - School of Public Health, The University of Hong Kong, Hong Kong, China. FAU - Balasuriya, U B R AU - Balasuriya UBR AD - Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, USA. AD - Department of Microbiology, Immunology and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY, USA. FAU - Peiris, M AU - Peiris M AD - School of Public Health, The University of Hong Kong, Hong Kong, China. LA - eng SI - GENBANK/EF446615 GR - HHSN272201500006C/AI/NIAID NIH HHS/United States PT - Journal Article DEP - 20170313 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 SB - IM MH - Animals MH - Betacoronavirus/genetics/*immunology/isolation & purification MH - Chlorocebus aethiops MH - Coronavirus/genetics/*immunology/isolation & purification MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Cross Reactions MH - Horse Diseases/*epidemiology/virology MH - Horses MH - Middle East Respiratory Syndrome Coronavirus/genetics/*immunology/isolation & purification MH - Oman/epidemiology MH - Saudi Arabia/epidemiology MH - Vero Cells PMC - PMC7169745 OTO - NOTNLM OT - HKU23 OT - cross-neutralization OT - equine coronavirus OT - middle east respiratory syndrome coronavirus OT - polymerase chain reaction OT - serology COIS- We declare that we have no conflict of interests. EDAT- 2017/03/16 06:00 MHDA- 2018/05/12 06:00 PMCR- 2020/04/20 CRDT- 2017/03/16 06:00 PHST- 2016/11/07 00:00 [received] PHST- 2017/03/16 06:00 [pubmed] PHST- 2018/05/12 06:00 [medline] PHST- 2017/03/16 06:00 [entrez] PHST- 2020/04/20 00:00 [pmc-release] AID - TBED12630 [pii] AID - 10.1111/tbed.12630 [doi] PST - ppublish SO - Transbound Emerg Dis. 2017 Dec;64(6):2093-2103. doi: 10.1111/tbed.12630. Epub 2017 Mar 13. PMID- 28725945 OWN - NLM STAT- MEDLINE DCOM- 20190116 LR - 20200324 IS - 1432-184X (Electronic) IS - 0095-3628 (Print) IS - 0095-3628 (Linking) VI - 75 IP - 1 DP - 2018 Jan TI - Genetic Characteristics of Coronaviruses from Korean Bats in 2016. PG - 174-182 LID - 10.1007/s00248-017-1033-8 [doi] AB - Bats have increasingly been recognized as the natural reservoir of severe acute respiratory syndrome (SARS), coronavirus, and other coronaviruses found in mammals. However, little research has been conducted on bat coronaviruses in South Korea. In this study, bat samples (332 oral swabs, 245 fecal samples, 38 urine samples, and 57 bat carcasses) were collected at 33 natural bat habitat sites in South Korea. RT-PCR and sequencing were performed for specific coronavirus genes to identify the bat coronaviruses in different bat samples. Coronaviruses were detected in 2.7% (18/672) of the samples: 13 oral swabs from one species of the family Rhinolophidae, and four fecal samples and one carcass (intestine) from three species of the family Vespertiliodae. To determine the genetic relationships of the 18 sequences obtained in this study and previously known coronaviruses, the nucleotide sequences of a 392-nt region of the RNA-dependent RNA polymerase (RdRp) gene were analyzed phylogenetically. Thirteen sequences belonging to SARS-like betacoronaviruses showed the highest nucleotide identity (97.1-99.7%) with Bat-CoV-JTMC15 reported in China. The other five sequences were most similar to MERS-like betacoronaviruses. Four nucleotide sequences displayed the highest identity (94.1-95.1%) with Bat-CoV-HKU5 from Hong Kong. The one sequence from a carcass showed the highest nucleotide identity (99%) with Bat-CoV-SC2013 from China. These results suggest that careful surveillance of coronaviruses from bats should be continued, because animal and human infections may result from the genetic variants present in bat coronavirus reservoirs. FAU - Lee, Saemi AU - Lee S AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. FAU - Jo, Seong-Deok AU - Jo SD AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. FAU - Son, Kidong AU - Son K AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. FAU - An, Injung AU - An I AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. FAU - Jeong, Jipseol AU - Jeong J AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. FAU - Wang, Seung-Jun AU - Wang SJ AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. FAU - Kim, Yongkwan AU - Kim Y AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. FAU - Jheong, Weonhwa AU - Jheong W AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. FAU - Oem, Jae-Ku AU - Oem JK AUID- ORCID: 0000-0002-4298-0604 AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Republic of Korea. jku0623@korea.kr. AD - Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of Korea. jku0623@korea.kr. LA - eng PT - Journal Article DEP - 20170719 PL - United States TA - Microb Ecol JT - Microbial ecology JID - 7500663 SB - IM MH - Animals MH - China MH - Chiroptera/*virology MH - Coronavirus/classification/genetics/*isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - Genetic Variation MH - Genome, Viral MH - Hong Kong MH - Humans MH - Phylogeny MH - Republic of Korea PMC - PMC7079938 OTO - NOTNLM OT - Bats OT - Coronavirus OT - Middle East respiratory syndrome OT - Phylogenetic analysis OT - Severe acute respiratory syndrome OT - South Korea EDAT- 2017/07/21 06:00 MHDA- 2019/01/17 06:00 PMCR- 2020/03/18 CRDT- 2017/07/21 06:00 PHST- 2017/02/24 00:00 [received] PHST- 2017/07/04 00:00 [accepted] PHST- 2017/07/21 06:00 [pubmed] PHST- 2019/01/17 06:00 [medline] PHST- 2017/07/21 06:00 [entrez] PHST- 2020/03/18 00:00 [pmc-release] AID - 10.1007/s00248-017-1033-8 [pii] AID - 1033 [pii] AID - 10.1007/s00248-017-1033-8 [doi] PST - ppublish SO - Microb Ecol. 2018 Jan;75(1):174-182. doi: 10.1007/s00248-017-1033-8. Epub 2017 Jul 19. PMID- 27786402 OWN - NLM STAT- MEDLINE DCOM- 20170823 LR - 20200422 IS - 1099-1654 (Electronic) IS - 1052-9276 (Print) IS - 1052-9276 (Linking) VI - 27 IP - 2 DP - 2017 Mar TI - Vaccines against Middle East respiratory syndrome coronavirus for humans and camels. LID - 10.1002/rmv.1917 [doi] LID - e1917 AB - Middle East respiratory syndrome coronavirus (MERS-CoV) is caused by a novel betacoronavirus that was isolated in late 2012 in Saudi Arabia. The viral infections have been reported in more than 1700 humans, ranging from asymptomatic or mild cases to severe pneumonia with a mortality rate of 40%. It is well documented now that dromedary camels contract the infection and shed the virus without notable symptoms, and such animals had been infected by at least the early 1980s. The mechanism of camel to human transmission is still not clear, but several primary cases have been associated with camel contact. There is no approved antiviral drug or vaccine against MERS-CoV despite the active research in this area. Vaccine candidates have been developed using various platforms and regimens and have been tested in several animal models. Here, this article reviews the published studies on MERS-CoV vaccines with more focus on vaccines tested in large animals, including camels. It is foreseeable that the 1-health approach could be the best way of tackling the MERS-CoV endemic in the Arabian Peninsula, by using the mass vaccination of camels in the affected areas to block camel to human transmission. Camel vaccines can be developed in a faster time with fewer regulations and lower costs and could clear this virus from the Arabian Peninsula if accompanied by efficient public health measures. CI - Copyright © 2016 John Wiley & Sons, Ltd. FAU - Alharbi, Naif Khalaf AU - Alharbi NK AUID- ORCID: 0000-0002-6049-3383 AD - Infectious Diseases Research Department, King Abdullah International Medical Research Center (KAIMRC), Riyadh, Saudi Arabia. AD - The Jenner Institute, University of Oxford, Oxford, United Kingdom. LA - eng PT - Journal Article PT - Review DEP - 20161027 PL - England TA - Rev Med Virol JT - Reviews in medical virology JID - 9112448 RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Camelus MH - Coronavirus Infections/immunology/*prevention & control MH - Drug Evaluation, Preclinical MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*immunology MH - Saudi Arabia MH - Viral Vaccines/*immunology/*isolation & purification PMC - PMC7169231 OTO - NOTNLM OT - MERS coronavirus vaccine OT - Middle East Respiratory Syndrome OT - camel vaccine EDAT- 2016/10/28 06:00 MHDA- 2017/08/24 06:00 PMCR- 2020/04/20 CRDT- 2016/10/28 06:00 PHST- 2016/04/25 00:00 [received] PHST- 2016/09/01 00:00 [revised] PHST- 2016/09/14 00:00 [accepted] PHST- 2016/10/28 06:00 [pubmed] PHST- 2017/08/24 06:00 [medline] PHST- 2016/10/28 06:00 [entrez] PHST- 2020/04/20 00:00 [pmc-release] AID - RMV1917 [pii] AID - 10.1002/rmv.1917 [doi] PST - ppublish SO - Rev Med Virol. 2017 Mar;27(2):e1917. doi: 10.1002/rmv.1917. Epub 2016 Oct 27. PMID- 26920708 OWN - NLM STAT- MEDLINE DCOM- 20161213 LR - 20221207 IS - 1995-820X (Electronic) IS - 1674-0769 (Print) IS - 1995-820X (Linking) VI - 31 IP - 1 DP - 2016 Feb TI - Coexistence of multiple coronaviruses in several bat colonies in an abandoned mineshaft. PG - 31-40 LID - 10.1007/s12250-016-3713-9 [doi] AB - Since the 2002-2003 severe acute respiratory syndrome (SARS) outbreak prompted a search for the natural reservoir of the SARS coronavirus, numerous alpha- and betacoronaviruses have been discovered in bats around the world. Bats are likely the natural reservoir of alpha- and betacoronaviruses, and due to the rich diversity and global distribution of bats, the number of bat coronaviruses will likely increase. We conducted a surveillance of coronaviruses in bats in an abandoned mineshaft in Mojiang County, Yunnan Province, China, from 2012-2013. Six bat species were frequently detected in the cave: Rhinolophus sinicus, Rhinolophus affinis, Hipposideros pomona, Miniopterus schreibersii, Miniopterus fuliginosus, and Miniopterus fuscus. By sequencing PCR products of the coronavirus RNA-dependent RNA polymerase gene (RdRp), we found a high frequency of infection by a diverse group of coronaviruses in different bat species in the mineshaft. Sequenced partial RdRp fragments had 80%-99% nucleic acid sequence identity with well-characterized Alphacoronavirus species, including BtCoV HKU2, BtCoV HKU8, and BtCoV1, and unassigned species BtCoV HKU7 and BtCoV HKU10. Additionally, the surveillance identified two unclassified betacoronaviruses, one new strain of SARS-like coronavirus, and one potentially new betacoronavirus species. Furthermore, coronavirus co-infection was detected in all six bat species, a phenomenon that fosters recombination and promotes the emergence of novel virus strains. Our findings highlight the importance of bats as natural reservoirs of coronaviruses and the potentially zoonotic source of viral pathogens. FAU - Ge, Xing-Yi AU - Ge XY AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Wang, Ning AU - Wang N AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Zhang, Wei AU - Zhang W AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Hu, Ben AU - Hu B AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Li, Bei AU - Li B AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Zhang, Yun-Zhi AU - Zhang YZ AD - Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. AD - School of Public Health, Dali University, Dali, 671000, China. FAU - Zhou, Ji-Hua AU - Zhou JH AD - Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. FAU - Luo, Chu-Ming AU - Luo CM AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Yang, Xing-Lou AU - Yang XL AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Wu, Li-Jun AU - Wu LJ AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Wang, Bo AU - Wang B AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Zhang, Yun AU - Zhang Y AD - Mojiang Center for Diseases Control and Prevention, Mojiang, 654800, China. FAU - Li, Zong-Xiao AU - Li ZX AD - Mojiang Center for Diseases Control and Prevention, Mojiang, 654800, China. FAU - Shi, Zheng-Li AU - Shi ZL AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160218 PL - Netherlands TA - Virol Sin JT - Virologica Sinica JID - 101514185 RN - 0 (RNA, Viral) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - China/epidemiology MH - Chiroptera/*virology MH - Coronavirus/genetics/*isolation & purification MH - Coronavirus Infections/epidemiology/veterinary/*virology MH - Genome, Viral MH - Phylogeny MH - Polymerase Chain Reaction/methods MH - RNA, Viral/genetics MH - RNA-Dependent RNA Polymerase/genetics MH - Severe acute respiratory syndrome-related coronavirus/genetics/isolation & purification MH - Sequence Analysis, DNA MH - Severe Acute Respiratory Syndrome/epidemiology/genetics/veterinary PMC - PMC7090819 OTO - NOTNLM OT - bat OT - coinfection OT - coronavirus OT - mineshaft EDAT- 2016/02/28 06:00 MHDA- 2016/12/15 06:00 PMCR- 2017/02/18 CRDT- 2016/02/28 06:00 PHST- 2016/01/01 00:00 [received] PHST- 2016/01/22 00:00 [accepted] PHST- 2016/02/28 06:00 [entrez] PHST- 2016/02/28 06:00 [pubmed] PHST- 2016/12/15 06:00 [medline] PHST- 2017/02/18 00:00 [pmc-release] AID - 10.1007/s12250-016-3713-9 [pii] AID - 3713 [pii] AID - 10.1007/s12250-016-3713-9 [doi] PST - ppublish SO - Virol Sin. 2016 Feb;31(1):31-40. doi: 10.1007/s12250-016-3713-9. Epub 2016 Feb 18. PMID- 28384506 OWN - NLM STAT- MEDLINE DCOM- 20170717 LR - 20200407 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 507 DP - 2017 Jul TI - Extensive diversity of coronaviruses in bats from China. PG - 1-10 LID - S0042-6822(17)30100-9 [pii] LID - 10.1016/j.virol.2017.03.019 [doi] AB - To help reveal the diversity and evolution of bat coronaviruses we collected 1067 bats from 21 species in China. A total of 73 coronaviruses (32 alphacoronaviruses and 41 betacoronaviruses) were identified in these bats, with an overall prevalence of 6.84%. All newly-identified betacoronaviruses were SARS-related Rhinolophus bat coronaviruses (SARSr-Rh-BatCoV). Importantly, with the exception of the S gene, the genome sequences of the SARSr-Rh-BatCoVs sampled in Guizhou province were closely related to SARS-related human coronavirus. Additionally, the newly-identified alphacoronaviruses exhibited high genetic diversity and some may represent novel species. Our phylogenetic analyses also provided insights into the transmission of these viruses among bat species, revealing a general clustering by geographic location rather than by bat species. Inter-species transmission among bats from the same genus was also commonplace in both the alphacoronaviruses and betacoronaviruses. Overall, these data suggest that high contact rates among specific bat species enable the acquisition and spread of coronaviruses. CI - Copyright © 2017 Elsevier Inc. All rights reserved. FAU - Lin, Xian-Dan AU - Lin XD AD - State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Wenzhou Center for Disease Control and Prevention, Wenzhou, Zhejiang Province, China. FAU - Wang, Wen AU - Wang W AD - State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. FAU - Hao, Zong-Yu AU - Hao ZY AD - Henan Center for Disease Control and Prevention, Zhengzhou, Henan Province, China. FAU - Wang, Zhao-Xiao AU - Wang ZX AD - Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou Province, China. FAU - Guo, Wen-Ping AU - Guo WP AD - State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. FAU - Guan, Xiao-Qing AU - Guan XQ AD - State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. FAU - Wang, Miao-Ruo AU - Wang MR AD - Longquan Center for Disease Control and Prevention, Longquan, Zhejiang Province, China. FAU - Wang, Hong-Wei AU - Wang HW AD - Neixiang Center for Disease Control and Prevention, Neixiang, Henan Province, China. FAU - Zhou, Run-Hong AU - Zhou RH AD - State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. FAU - Li, Ming-Hui AU - Li MH AD - State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. FAU - Tang, Guang-Peng AU - Tang GP AD - Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou Province, China. FAU - Wu, Jun AU - Wu J AD - Jiyuan Center for Disease Control and Prevention, Jiyuan, Henan Province, China. FAU - Holmes, Edward C AU - Holmes EC AD - Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia. FAU - Zhang, Yong-Zhen AU - Zhang YZ AD - State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. Electronic address: zhangyongzhen@icdc.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170403 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - China MH - Chiroptera/*virology MH - Coronavirus/classification/*genetics/*isolation & purification MH - *Genetic Variation MH - Genome, Viral MH - Phylogeny MH - RNA, Viral/genetics PMC - PMC7111643 OTO - NOTNLM OT - Bats OT - Coronavirus OT - Evolution OT - Phylogeny OT - SARS OT - Transmission EDAT- 2017/04/07 06:00 MHDA- 2017/07/18 06:00 PMCR- 2017/04/03 CRDT- 2017/04/07 06:00 PHST- 2017/02/12 00:00 [received] PHST- 2017/03/27 00:00 [revised] PHST- 2017/03/28 00:00 [accepted] PHST- 2017/04/07 06:00 [pubmed] PHST- 2017/07/18 06:00 [medline] PHST- 2017/04/07 06:00 [entrez] PHST- 2017/04/03 00:00 [pmc-release] AID - S0042-6822(17)30100-9 [pii] AID - 10.1016/j.virol.2017.03.019 [doi] PST - ppublish SO - Virology. 2017 Jul;507:1-10. doi: 10.1016/j.virol.2017.03.019. Epub 2017 Apr 3. PMID- 25463600 OWN - NLM STAT- MEDLINE DCOM- 20150424 LR - 20240727 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 474 DP - 2015 Jan 1 TI - Discovery, diversity and evolution of novel coronaviruses sampled from rodents in China. PG - 19-27 LID - S0042-6822(14)00472-3 [pii] LID - 10.1016/j.virol.2014.10.017 [doi] AB - Although rodents are important reservoirs for RNA viruses, to date only one species of rodent coronavirus (CoV) has been identified. Herein, we describe a new CoV, denoted Lucheng Rn rat coronavirus (LRNV), and novel variants of two Betacoronavirus species termed Longquan Aa mouse coronavirus (LAMV) and Longquan Rl rat coronavirus (LRLV), that were identified in a survey of 1465 rodents sampled in China during 2011-2013. Phylogenetic analysis revealed that LAMV and LRLV fell into lineage A of the genus Betacoronavirus, which included CoVs discovered in humans and domestic and wild animals. In contrast, LRNV harbored by Rattus norvegicus formed a distinct lineage within the genus Alphacoronavirus in the 3CL(pro), RdRp, and Hel gene trees, but formed a more divergent lineage in the N and S gene trees, indicative of a recombinant origin. Additional recombination events were identified in LRLV. Together, these data suggest that rodents may carry additional unrecognized CoVs. CI - Copyright © 2014 Elsevier Inc. All rights reserved. FAU - Wang, Wen AU - Wang W AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China. FAU - Lin, Xian-Dan AU - Lin XD AD - Wenzhou Center for Disease Control and Prevention, Wenzhou, Zhejiang Province, China. FAU - Guo, Wen-Ping AU - Guo WP AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China. FAU - Zhou, Run-Hong AU - Zhou RH AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. FAU - Wang, Miao-Ruo AU - Wang MR AD - Longquan Center for Disease Control and Prevention, Longquan, Zhejiang Province, China. FAU - Wang, Cai-Qiao AU - Wang CQ AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. FAU - Ge, Shuang AU - Ge S AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China. FAU - Mei, Sheng-Hua AU - Mei SH AD - Longquan Center for Disease Control and Prevention, Longquan, Zhejiang Province, China. FAU - Li, Ming-Hui AU - Li MH AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China. FAU - Shi, Mang AU - Shi M AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Marie Bashir Institute of Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Biological Sciences and Sydney Medical School, The University of Sydney, Sydney, New South Wales 2006, Australia. FAU - Holmes, Edward C AU - Holmes EC AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Marie Bashir Institute of Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Biological Sciences and Sydney Medical School, The University of Sydney, Sydney, New South Wales 2006, Australia. FAU - Zhang, Yong-Zhen AU - Zhang YZ AD - State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China. Electronic address: zhangyongzhen@icdc.cn. LA - eng SI - GENBANK/KF294345 SI - GENBANK/KF294346 SI - GENBANK/KF294347 SI - GENBANK/KF294348 SI - GENBANK/KF294349 SI - GENBANK/KF294350 SI - GENBANK/KF294351 SI - GENBANK/KF294352 SI - GENBANK/KF294353 SI - GENBANK/KF294354 SI - GENBANK/KF294355 SI - GENBANK/KF294356 SI - GENBANK/KF294357 SI - GENBANK/KF294358 SI - GENBANK/KF294359 SI - GENBANK/KF294360 SI - GENBANK/KF294361 SI - GENBANK/KF294362 SI - GENBANK/KF294363 SI - GENBANK/KF294364 SI - GENBANK/KF294365 SI - GENBANK/KF294366 SI - GENBANK/KF294367 SI - GENBANK/KF294368 SI - GENBANK/KF294369 SI - GENBANK/KF294370 SI - GENBANK/KF294371 SI - GENBANK/KF294372 SI - GENBANK/KF294379 SI - GENBANK/KF294380 SI - GENBANK/KF294387 SI - GENBANK/KF294388 SI - GENBANK/KF294389 SI - GENBANK/KF294390 SI - GENBANK/KF294391 SI - GENBANK/KF294392 SI - GENBANK/KF294393 SI - GENBANK/KF294394 SI - GENBANK/KF294395 SI - GENBANK/KF294396 SI - GENBANK/KF294397 SI - GENBANK/KF294398 SI - GENBANK/KF294399 SI - GENBANK/KF294400 SI - GENBANK/KF294401 SI - GENBANK/KF294402 SI - GENBANK/KF294403 SI - GENBANK/KF294404 SI - GENBANK/KF294405 SI - GENBANK/KF294406 SI - GENBANK/KF294407 SI - GENBANK/KF294408 SI - GENBANK/KF294409 SI - GENBANK/KF294410 SI - GENBANK/KF294411 SI - GENBANK/KF294412 SI - GENBANK/KF294413 SI - GENBANK/KF294414 SI - GENBANK/KF294415 SI - GENBANK/KF294416 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141109 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - China MH - Coronavirus/classification/*genetics/*isolation & purification MH - Coronavirus, Rat/classification/genetics/isolation & purification MH - Disease Reservoirs/virology MH - Evolution, Molecular MH - Genetic Variation MH - Genome, Viral MH - Humans MH - Mice MH - Molecular Sequence Data MH - Phylogeny MH - Rats MH - Rodentia/*virology MH - Spike Glycoprotein, Coronavirus/chemistry/genetics PMC - PMC7112057 OTO - NOTNLM OT - Coronavirus OT - Evolution OT - Phylogeny OT - Recombination OT - Rodents EDAT- 2014/12/03 06:00 MHDA- 2015/04/25 06:00 PMCR- 2014/11/09 CRDT- 2014/12/03 06:00 PHST- 2014/07/17 00:00 [received] PHST- 2014/09/23 00:00 [revised] PHST- 2014/10/17 00:00 [accepted] PHST- 2014/12/03 06:00 [entrez] PHST- 2014/12/03 06:00 [pubmed] PHST- 2015/04/25 06:00 [medline] PHST- 2014/11/09 00:00 [pmc-release] AID - S0042-6822(14)00472-3 [pii] AID - 10.1016/j.virol.2014.10.017 [doi] PST - ppublish SO - Virology. 2015 Jan 1;474:19-27. doi: 10.1016/j.virol.2014.10.017. Epub 2014 Nov 9. PMID- 30632200 OWN - NLM STAT- MEDLINE DCOM- 20190429 LR - 20231006 IS - 1939-1676 (Electronic) IS - 0891-6640 (Print) IS - 0891-6640 (Linking) VI - 33 IP - 2 DP - 2019 Mar TI - Disease features of equine coronavirus and enteric salmonellosis are similar in horses. PG - 912-917 LID - 10.1111/jvim.15386 [doi] AB - BACKGROUND: Equine coronavirus (ECoV) is an emerging pathogen associated with fever and enteric disease in adult horses. Clinical features of ECoV infection have been described, but no study has compared these features to those of Salmonella infections. OBJECTIVES: Compare the clinical features of ECoV infection with enteric salmonellosis and establish a disease signature to increase clinical suspicion of ECoV infection in adult horses. ANIMALS: Forty-three horses >1 year of age with results of CBC, serum biochemistry, and fecal diagnostic testing for ECoV and Salmonella spp. METHODS: Medical records of horses presented to the North Carolina State University Equine and Farm Animal Veterinary Center (2003-016) were retrospectively reviewed. Horses were divided into 3 groups based on fecal diagnostic test results: ECoV-positive, Salmonella-positive, or unknown diagnosis (UNK). Time of year presented, clinical signs, CBC, and serum biochemistry test results were recorded. Data were analyzed by 1-way analysis of variance, Kruskal-Wallis test, or Fisher's exact test with significance set at P < .05. RESULTS: Most common presenting complaints were fever and colic and were similar across groups. Horses with ECoV had significantly decreased neutrophil counts when compared to those with no diagnosis but were not different from horses with Salmonella. Horses with Salmonella had significantly lower mean leukocyte counts compared to those with UNK. No significant differences were found among groups for any other examined variable. CONCLUSIONS AND CLINICAL IMPORTANCE: Equine coronavirus and Salmonella infections share clinical features, suggesting both diseases should be differential diagnoses for horses with fever and enteric clinical signs. CI - © 2019 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine. FAU - Manship, Arlie J AU - Manship AJ AD - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina. FAU - Blikslager, Anthony T AU - Blikslager AT AUID- ORCID: 0000-0002-0867-7310 AD - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina. FAU - Elfenbein, Johanna R AU - Elfenbein JR AUID- ORCID: 0000-0002-4764-0713 AD - Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina. LA - eng PT - Comparative Study PT - Journal Article DEP - 20190110 PL - United States TA - J Vet Intern Med JT - Journal of veterinary internal medicine JID - 8708660 SB - IM MH - Animals MH - Betacoronavirus 1/isolation & purification MH - Blood Cell Count/veterinary MH - Blood Chemical Analysis/veterinary MH - Communicable Diseases, Emerging MH - Coronavirus Infections/diagnosis/*veterinary MH - Diagnosis, Differential MH - Feces/microbiology MH - Female MH - Horse Diseases/*diagnosis/microbiology MH - Horses MH - Male MH - Retrospective Studies MH - Salmonella/isolation & purification MH - Salmonella Infections, Animal/*diagnosis PMC - PMC6430874 OTO - NOTNLM OT - colic OT - equine coronavirus OT - fever OT - salmonella COIS- Authors declare no conflict of interest. EDAT- 2019/01/12 06:00 MHDA- 2019/04/30 06:00 PMCR- 2019/03/01 CRDT- 2019/01/12 06:00 PHST- 2018/05/29 00:00 [received] PHST- 2018/11/14 00:00 [accepted] PHST- 2019/01/12 06:00 [pubmed] PHST- 2019/04/30 06:00 [medline] PHST- 2019/01/12 06:00 [entrez] PHST- 2019/03/01 00:00 [pmc-release] AID - JVIM15386 [pii] AID - 10.1111/jvim.15386 [doi] PST - ppublish SO - J Vet Intern Med. 2019 Mar;33(2):912-917. doi: 10.1111/jvim.15386. Epub 2019 Jan 10. PMID- 25211075 OWN - NLM STAT- MEDLINE DCOM- 20150616 LR - 20240328 IS - 1934-6069 (Electronic) IS - 1931-3128 (Print) IS - 1931-3128 (Linking) VI - 16 IP - 3 DP - 2014 Sep 10 TI - Bat origins of MERS-CoV supported by bat coronavirus HKU4 usage of human receptor CD26. PG - 328-37 LID - S1931-3128(14)00301-1 [pii] LID - 10.1016/j.chom.2014.08.009 [doi] AB - The recently reported Middle East respiratory syndrome coronavirus (MERS-CoV) is phylogenetically closely related to the bat coronaviruses (BatCoVs) HKU4 and HKU5. However, the evolutionary pathway of MERS-CoV is still unclear. A receptor binding domain (RBD) in the MERS-CoV envelope-embedded spike protein specifically engages human CD26 (hCD26) to initiate viral entry. The high sequence identity in the viral spike protein prompted us to investigate if HKU4 and HKU5 can recognize hCD26 for cell entry. We found that HKU4-RBD, but not HKU5-RBD, binds to hCD26, and pseudotyped viruses embedding HKU4 spike can infect cells via hCD26 recognition. The structure of the HKU4-RBD/hCD26 complex revealed a hCD26-binding mode similar overall to that observed for MERS-RBD. HKU4-RBD, however, is less adapted to hCD26 than MERS-RBD, explaining its lower affinity for receptor binding. Our findings support a bat origin for MERS-CoV and indicate the need for surveillance of HKU4-related viruses in bats. CI - Copyright © 2014 Elsevier Inc. All rights reserved. FAU - Wang, Qihui AU - Wang Q AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Qi, Jianxun AU - Qi J AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Yuan, Yuan AU - Yuan Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; School of Life Sciences, University of Science and Technology of China, Hefei 230027, Anhui Province, China. FAU - Xuan, Yifang AU - Xuan Y AD - Research Network of Immunity and Health, Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China. FAU - Han, Pengcheng AU - Han P AD - State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Wan, Yuhua AU - Wan Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; School of Life Sciences, Anhui University, Hefei 230039, China. FAU - Ji, Wei AU - Ji W AD - National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China. FAU - Li, Yan AU - Li Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Wu, Ying AU - Wu Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Wang, Jianwei AU - Wang J AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. FAU - Iwamoto, Aikichi AU - Iwamoto A AD - China-Japan Joint Laboratory of Molecular Microbiology and Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Division of Infectious Diseases, Advanced Clinical Research Center, Department of Infectious Diseases and Applied Immunology, Research Hospital, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan. FAU - Woo, Patrick C Y AU - Woo PC AD - State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region 999077, China; Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region 999077, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region 999077, China; Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region 999077, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, China. FAU - Yan, Jinghua AU - Yan J AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Lu, Guangwen AU - Lu G AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Gao, George F AU - Gao GF AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; School of Life Sciences, University of Science and Technology of China, Hefei 230027, Anhui Province, China; Research Network of Immunity and Health, Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310003, China; Office of Director-General, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China. Electronic address: gaof@im.ac.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cell Host Microbe JT - Cell host & microbe JID - 101302316 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Amino Acid Sequence MH - Animals MH - Chiroptera/*virology MH - Coronavirus/classification/genetics/isolation & purification/*metabolism MH - Coronavirus Infections/genetics/*metabolism/virology MH - Dipeptidyl Peptidase 4/genetics/*metabolism MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/classification/genetics/*metabolism MH - Molecular Sequence Data MH - Phylogeny MH - Protein Binding MH - Receptors, Virus/chemistry/genetics/*metabolism MH - Sequence Alignment MH - Spike Glycoprotein, Coronavirus/chemistry/genetics/metabolism PMC - PMC7104937 EDAT- 2014/09/12 06:00 MHDA- 2015/06/17 06:00 PMCR- 2014/09/11 CRDT- 2014/09/12 06:00 PHST- 2014/05/14 00:00 [received] PHST- 2014/07/30 00:00 [revised] PHST- 2014/08/22 00:00 [accepted] PHST- 2014/09/12 06:00 [entrez] PHST- 2014/09/12 06:00 [pubmed] PHST- 2015/06/17 06:00 [medline] PHST- 2014/09/11 00:00 [pmc-release] AID - S1931-3128(14)00301-1 [pii] AID - 10.1016/j.chom.2014.08.009 [doi] PST - ppublish SO - Cell Host Microbe. 2014 Sep 10;16(3):328-37. doi: 10.1016/j.chom.2014.08.009. PMID- 26177344 OWN - NLM STAT- MEDLINE DCOM- 20160222 LR - 20181113 IS - 1080-6059 (Electronic) IS - 1080-6040 (Print) IS - 1080-6040 (Linking) VI - 21 IP - 8 DP - 2015 Aug TI - Human-Bat Interactions in Rural West Africa. PG - 1418-21 LID - 10.3201/eid2108.142015 [doi] AB - Because some bats host viruses with zoonotic potential, we investigated human-bat interactions in rural Ghana during 2011-2012. Nearly half (46.6%) of respondents regularly visited bat caves; 37.4% had been bitten, scratched, or exposed to bat urine; and 45.6% ate bat meat. Human-bat interactions in rural Ghana are frequent and diverse. FAU - Anti, Priscilla AU - Anti P FAU - Owusu, Michael AU - Owusu M FAU - Agbenyega, Olivia AU - Agbenyega O FAU - Annan, Augustina AU - Annan A FAU - Badu, Ebenezer Kofi AU - Badu EK FAU - Nkrumah, Evans Ewald AU - Nkrumah EE FAU - Tschapka, Marco AU - Tschapka M FAU - Oppong, Samuel AU - Oppong S FAU - Adu-Sarkodie, Yaw AU - Adu-Sarkodie Y FAU - Drosten, Christian AU - Drosten C LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Emerg Infect Dis JT - Emerging infectious diseases JID - 9508155 SB - IM MH - Animal Diseases/epidemiology/*transmission MH - Animals MH - Chiroptera/*virology MH - Coronavirus Infections/*transmission/virology MH - Diet, Paleolithic/*adverse effects MH - Disease Reservoirs/statistics & numerical data/virology MH - *Disease Vectors MH - Ghana/epidemiology MH - Humans MH - *Rural Population MH - Zoonoses/*transmission/virology PMC - PMC4517717 OTO - NOTNLM OT - Ebola OT - MERS OT - Middle East respiratory syndrome OT - Nipah OT - SARS OT - West Africa OT - bats OT - severe acute respiratory syndrome OT - virus reservoir OT - viruses OT - zoonoses EDAT- 2015/07/16 06:00 MHDA- 2016/02/24 06:00 PMCR- 2015/08/01 CRDT- 2015/07/16 06:00 PHST- 2015/07/16 06:00 [entrez] PHST- 2015/07/16 06:00 [pubmed] PHST- 2016/02/24 06:00 [medline] PHST- 2015/08/01 00:00 [pmc-release] AID - 14-2015 [pii] AID - 10.3201/eid2108.142015 [doi] PST - ppublish SO - Emerg Infect Dis. 2015 Aug;21(8):1418-21. doi: 10.3201/eid2108.142015. PMID- 29186061 OWN - NLM STAT- MEDLINE DCOM- 20180716 LR - 20181113 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 9 IP - 12 DP - 2017 Nov 29 TI - Identification of Alpha and Beta Coronavirus in Wildlife Species in France: Bats, Rodents, Rabbits, and Hedgehogs. LID - 10.3390/v9120364 [doi] LID - 364 AB - Coronaviruses are closely monitored in the context of emerging diseases and, as illustrated with Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) and Middle East Respiratory Syndrome-coronavirus (MERS-CoV), are known to cross the species barrier and eventually to move from wildlife to humans. Knowledge of the diversity of coronaviruses in wildlife is therefore essential to better understand and prevent emergence events. This study explored the presence of coronaviruses in four wild mammal orders in France: Bats, rodents, lagomorphs, and hedgehogs. Betacoronavirus and Alphacoronavirus genera were identified. The results obtained suggest the circulation of potentially evolving virus strains, with the potential to cross the species barrier. FAU - Monchatre-Leroy, Elodie AU - Monchatre-Leroy E AUID- ORCID: 0000-0002-3231-398X AD - ANSES, Laboratoire de la rage et de la faune sauvage, 54220 Nancy, France. elodie.monchatre-leroy@anses.fr. FAU - Boué, Franck AU - Boué F AD - ANSES, Laboratoire de la rage et de la faune sauvage, 54220 Nancy, France. franck.boue@anses.fr. FAU - Boucher, Jean-Marc AU - Boucher JM AD - ANSES, Laboratoire de la rage et de la faune sauvage, 54220 Nancy, France. jean-marc.boucher@anses.fr. FAU - Renault, Camille AU - Renault C AD - ANSES, Laboratoire de la rage et de la faune sauvage, 54220 Nancy, France. camille.renault20@gmail.com. FAU - Moutou, François AU - Moutou F AD - ANSES, ENVA, 94701 Maisons-Alfort, France. francoismoutou@orange.fr. FAU - Ar Gouilh, Meriadeg AU - Ar Gouilh M AUID- ORCID: 0000-0002-3672-9974 AD - Université de Normandie, EA 2656, GRAM-Groupe de Recherche sur l'Adaptation Microbienne, UNICAEN/UNIROUEN, 14000 Caen, France. meriadeg.legouil@normandie-univ.fr. AD - Institut Pasteur, Infection et Epidemiologie, Unité Environnement et Risques Infectieux, 75015 Paris, France. meriadeg.legouil@normandie-univ.fr. FAU - Umhang, Gérald AU - Umhang G AUID- ORCID: 0000-0001-6944-7474 AD - ANSES, Laboratoire de la rage et de la faune sauvage, 54220 Nancy, France. gerald.umhang@anses.fr. LA - eng PT - Journal Article DEP - 20171129 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Alphacoronavirus/classification/isolation & purification MH - Animals MH - Betacoronavirus/classification/isolation & purification MH - Chiroptera/virology MH - Coronavirus/*classification/genetics/isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - Disease Reservoirs/*virology MH - France MH - *Genetic Variation MH - Geography MH - Hedgehogs MH - Phylogeny MH - Rabbits MH - Rodentia PMC - PMC5744139 OTO - NOTNLM OT - France OT - bats OT - coronavirus OT - genetic diversity OT - hedgehogs OT - rodents OT - wild rabbits OT - wildlife COIS- The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. EDAT- 2017/12/01 06:00 MHDA- 2018/07/17 06:00 PMCR- 2017/12/01 CRDT- 2017/11/30 06:00 PHST- 2017/11/02 00:00 [received] PHST- 2017/11/20 00:00 [revised] PHST- 2017/11/21 00:00 [accepted] PHST- 2017/11/30 06:00 [entrez] PHST- 2017/12/01 06:00 [pubmed] PHST- 2018/07/17 06:00 [medline] PHST- 2017/12/01 00:00 [pmc-release] AID - v9120364 [pii] AID - viruses-09-00364 [pii] AID - 10.3390/v9120364 [doi] PST - epublish SO - Viruses. 2017 Nov 29;9(12):364. doi: 10.3390/v9120364. PMID- 25552712 OWN - NLM STAT- MEDLINE DCOM- 20150526 LR - 20181113 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 6 DP - 2015 Mar TI - Discovery of a novel coronavirus, China Rattus coronavirus HKU24, from Norway rats supports the murine origin of Betacoronavirus 1 and has implications for the ancestor of Betacoronavirus lineage A. PG - 3076-92 LID - 10.1128/JVI.02420-14 [doi] AB - We discovered a novel Betacoronavirus lineage A coronavirus, China Rattus coronavirus (ChRCoV) HKU24, from Norway rats in China. ChRCoV HKU24 occupied a deep branch at the root of members of Betacoronavirus 1, being distinct from murine coronavirus and human coronavirus HKU1. Its unique putative cleavage sites between nonstructural proteins 1 and 2 and in the spike (S) protein and low sequence identities to other lineage A betacoronaviruses (βCoVs) in conserved replicase domains support ChRCoV HKU24 as a separate species. ChRCoV HKU24 possessed genome features that resemble those of both Betacoronavirus 1 and murine coronavirus, being closer to Betacoronavirus 1 in most predicted proteins but closer to murine coronavirus by G+C content, the presence of a single nonstructural protein (NS4), and an absent transcription regulatory sequence for the envelope (E) protein. Its N-terminal domain (NTD) demonstrated higher sequence identity to the bovine coronavirus (BCoV) NTD than to the mouse hepatitis virus (MHV) NTD, with 3 of 4 critical sugar-binding residues in BCoV and 2 of 14 contact residues at the MHV NTD/murine CEACAM1a interface being conserved. Molecular clock analysis dated the time of the most recent common ancestor of ChRCoV HKU24, Betacoronavirus 1, and rabbit coronavirus HKU14 to about the year 1400. Cross-reactivities between other lineage A and B βCoVs and ChRCoV HKU24 nucleocapsid but not spike polypeptide were demonstrated. Using the spike polypeptide-based Western blot assay, we showed that only Norway rats and two oriental house rats from Guangzhou, China, were infected by ChRCoV HKU24. Other rats, including Norway rats from Hong Kong, possessed antibodies only against N protein and not against the spike polypeptide, suggesting infection by βCoVs different from ChRCoV HKU24. ChRCoV HKU24 may represent the murine origin of Betacoronavirus 1, and rodents are likely an important reservoir for ancestors of lineage A βCoVs. IMPORTANCE: While bats and birds are hosts for ancestors of most coronaviruses (CoVs), lineage A βCoVs have never been found in these animals and the origin of Betacoronavirus lineage A remains obscure. We discovered a novel lineage A βCoV, China Rattus coronavirus HKU24 (ChRCoV HKU24), from Norway rats in China with a high seroprevalence. The unique genome features and phylogenetic analysis supported the suggestion that ChRCoV HKU24 represents a novel CoV species, occupying a deep branch at the root of members of Betacoronavirus 1 and being distinct from murine coronavirus. Nevertheless, ChRCoV HKU24 possessed genome characteristics that resemble those of both Betacoronavirus 1 and murine coronavirus. Our data suggest that ChRCoV HKU24 represents the murine origin of Betacoronavirus 1, with interspecies transmission from rodents to other mammals having occurred centuries ago, before the emergence of human coronavirus (HCoV) OC43 in the late 1800s. Rodents are likely an important reservoir for ancestors of lineage A βCoVs. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Lau, Susanna K P AU - Lau SK AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Woo, Patrick C Y AU - Woo PC AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Li, Kenneth S M AU - Li KS AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Tsang, Alan K L AU - Tsang AK AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Fan, Rachel Y Y AU - Fan RY AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Luk, Hayes K H AU - Luk HK AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Cai, Jian-Piao AU - Cai JP AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Chan, Kwok-Hung AU - Chan KH AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Zheng, Bo-Jian AU - Zheng BJ AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Wang, Ming AU - Wang M AD - Guangzhou Center for Disease Control and Prevention, Guangzhou, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China kyyuen@hkucc.hku.hk. LA - eng SI - GENBANK/KM349742 SI - GENBANK/KM349743 SI - GENBANK/KM349744 SI - PDB/4H14 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141231 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Viral Proteins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Cattle MH - Coronaviridae/chemistry/*classification/genetics/*isolation & purification MH - Coronaviridae Infections/*veterinary/virology MH - *Evolution, Molecular MH - Genome, Viral MH - Humans MH - Mice MH - Molecular Sequence Data MH - Phylogeny MH - Rabbits MH - Rats/*virology MH - Rodent Diseases/*virology MH - Sequence Alignment MH - Viral Proteins/chemistry/genetics PMC - PMC4337523 EDAT- 2015/01/02 06:00 MHDA- 2015/05/27 06:00 PMCR- 2015/09/15 CRDT- 2015/01/02 06:00 PHST- 2015/01/02 06:00 [entrez] PHST- 2015/01/02 06:00 [pubmed] PHST- 2015/05/27 06:00 [medline] PHST- 2015/09/15 00:00 [pmc-release] AID - JVI.02420-14 [pii] AID - 02420-14 [pii] AID - 10.1128/JVI.02420-14 [doi] PST - ppublish SO - J Virol. 2015 Mar;89(6):3076-92. doi: 10.1128/JVI.02420-14. Epub 2014 Dec 31. PMID- 28446791 OWN - NLM STAT- MEDLINE DCOM- 20180906 LR - 20201209 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 7 IP - 1 DP - 2017 Apr 26 TI - Tissue Distribution of the MERS-Coronavirus Receptor in Bats. PG - 1193 LID - 10.1038/s41598-017-01290-6 [doi] LID - 1193 AB - Middle East respiratory syndrome coronavirus (MERS-CoV) has been shown to infect both humans and dromedary camels using dipeptidyl peptidase-4 (DPP4) as its receptor. The distribution of DPP4 in the respiratory tract tissues of humans and camels reflects MERS-CoV tropism. Apart from dromedary camels, insectivorous bats are suggested as another natural reservoir for MERS-like-CoVs. In order to gain insight on the tropism of these viruses in bats, we studied the DPP4 distribution in the respiratory and extra-respiratory tissues of two frugivorous bat species (Epomophorus gambianus and Rousettus aegyptiacus) and two insectivorous bat species (Pipistrellus pipistrellus and Eptesicus serotinus). In the frugivorous bats, DPP4 was present in epithelial cells of both the respiratory and the intestinal tract, similar to what has been reported for camels and humans. In the insectivorous bats, however, DPP4 expression in epithelial cells of the respiratory tract was almost absent. The preferential expression of DPP4 in the intestinal tract of insectivorous bats, suggests that transmission of MERS-like-CoVs mainly occurs via the fecal-oral route. Our results highlight differences in the distribution of DPP4 expression among MERS-CoV susceptible species, which might influence variability in virus tropism, pathogenesis and transmission route. FAU - Widagdo, W AU - Widagdo W AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Begeman, Lineke AU - Begeman L AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Schipper, Debby AU - Schipper D AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Run, Peter R van AU - Run PRV AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Cunningham, Andrew A AU - Cunningham AA AD - Institute of Zoology, Zoological Society of London, Regents Park, London, United Kingdom. FAU - Kley, Nils AU - Kley N AD - Institute for Novel and Emerging Infectious Diseases, Friedrich Loeffler Institute, Greifswald, Mecklenburg-Vorpommern, Germany. FAU - Reusken, Chantal B AU - Reusken CB AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. FAU - Haagmans, Bart L AU - Haagmans BL AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. b.haagmans@erasmusmc.nl. FAU - van den Brand, Judith M A AU - van den Brand JMA AD - Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170426 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Receptors, Coronavirus) RN - 0 (Receptors, Virus) SB - IM MH - Animals MH - *Chiroptera MH - Epithelial Cells/*chemistry MH - Intestinal Mucosa/*chemistry MH - Middle East Respiratory Syndrome Coronavirus/*physiology MH - Receptors, Coronavirus MH - Receptors, Virus/*analysis MH - Respiratory Mucosa/*chemistry MH - *Viral Tropism PMC - PMC5430768 COIS- The authors declare that they have no competing interests. EDAT- 2017/04/28 06:00 MHDA- 2018/09/07 06:00 PMCR- 2017/04/26 CRDT- 2017/04/28 06:00 PHST- 2017/01/26 00:00 [received] PHST- 2017/03/27 00:00 [accepted] PHST- 2017/04/28 06:00 [entrez] PHST- 2017/04/28 06:00 [pubmed] PHST- 2018/09/07 06:00 [medline] PHST- 2017/04/26 00:00 [pmc-release] AID - 10.1038/s41598-017-01290-6 [pii] AID - 1290 [pii] AID - 10.1038/s41598-017-01290-6 [doi] PST - epublish SO - Sci Rep. 2017 Apr 26;7(1):1193. doi: 10.1038/s41598-017-01290-6. PMID- 25643817 OWN - NLM STAT- MEDLINE DCOM- 20150526 LR - 20200325 IS - 1432-8798 (Electronic) IS - 0304-8608 (Print) IS - 0304-8608 (Linking) VI - 160 IP - 4 DP - 2015 Apr TI - Detection of coronavirus genomes in Moluccan naked-backed fruit bats in Indonesia. PG - 1113-8 LID - 10.1007/s00705-015-2342-1 [doi] AB - Bats have been shown to serve as natural reservoirs for numerous emerging viruses including severe acute respiratory syndrome coronavirus (SARS-CoV). In the present study, we report the discovery of bat CoV genes in Indonesian Moluccan naked-backed fruit bats (Dobsonia moluccensis). A partial RNA-dependent RNA polymerase gene sequence was detected in feces and tissues samples from the fruit bats, and the region between the RdRp and helicase genes could also be amplified from fecal samples. Phylogenetic analysis suggested that these bat CoVs are related to members of the genus Betacoronavirus. FAU - Anindita, Paulina Duhita AU - Anindita PD AD - Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, North 20 West 10, Kita-ku, Sapporo, 001-0020, Japan. FAU - Sasaki, Michihito AU - Sasaki M FAU - Setiyono, Agus AU - Setiyono A FAU - Handharyani, Ekowati AU - Handharyani E FAU - Orba, Yasuko AU - Orba Y FAU - Kobayashi, Shintaro AU - Kobayashi S FAU - Rahmadani, Ibnu AU - Rahmadani I FAU - Taha, Siswatiana AU - Taha S FAU - Adiani, Sri AU - Adiani S FAU - Subangkit, Mawar AU - Subangkit M FAU - Nakamura, Ichiro AU - Nakamura I FAU - Sawa, Hirofumi AU - Sawa H FAU - Kimura, Takashi AU - Kimura T LA - eng SI - GENBANK/AB918718 SI - GENBANK/AB918719 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150204 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronavirus/classification/*genetics/*isolation & purification MH - Disease Reservoirs/*virology MH - *Genome, Viral MH - Indonesia MH - Molecular Sequence Data MH - Phylogeny PMC - PMC7086880 EDAT- 2015/02/04 06:00 MHDA- 2015/05/27 06:00 PMCR- 2020/03/23 CRDT- 2015/02/04 06:00 PHST- 2014/10/01 00:00 [received] PHST- 2015/01/15 00:00 [accepted] PHST- 2015/02/04 06:00 [entrez] PHST- 2015/02/04 06:00 [pubmed] PHST- 2015/05/27 06:00 [medline] PHST- 2020/03/23 00:00 [pmc-release] AID - 2342 [pii] AID - 10.1007/s00705-015-2342-1 [doi] PST - ppublish SO - Arch Virol. 2015 Apr;160(4):1113-8. doi: 10.1007/s00705-015-2342-1. Epub 2015 Feb 4. PMID- 27473780 OWN - NLM STAT- MEDLINE DCOM- 20170926 LR - 20200605 IS - 1567-7257 (Electronic) IS - 1567-1348 (Print) IS - 1567-1348 (Linking) VI - 44 DP - 2016 Oct TI - Genetic diversity of bats coronaviruses in the Atlantic Forest hotspot biome, Brazil. PG - 510-513 LID - S1567-1348(16)30324-0 [pii] LID - 10.1016/j.meegid.2016.07.034 [doi] AB - Bats are notorious reservoirs of genetically-diverse and high-profile pathogens, and are playing crucial roles in the emergence and re-emergence of viruses, both in human and in animals. In this report, we identified and characterized previously unknown and diverse genetic clusters of bat coronaviruses in the Atlantic Forest Biome, Brazil. These results highlight the virus richness of bats and their possible roles in the public health. CI - Copyright © 2016 Elsevier B.V. All rights reserved. FAU - Góes, Luiz Gustavo Bentim AU - Góes LGB AD - Universidade de São Paulo (USP), Dept. Microbiologia, Instituto de Ciências Biomédicas (ICB), Sao Paulo, SP, Brazil. Electronic address: lgbgoes@usp.br. FAU - Campos, Angélica Cristine de Almeida AU - Campos ACA AD - Universidade de São Paulo (USP), Dept. Microbiologia, Instituto de Ciências Biomédicas (ICB), Sao Paulo, SP, Brazil. Electronic address: camposac@usp.br. FAU - Carvalho, Cristiano de AU - Carvalho C AD - Universidade Estadual Paulista (UNESP), Dept. de Apoio, Produção e Saúde Animal, Faculdade de Medicina Veterinária de Araçatuba, Araçatuba, SP, Brazil. Electronic address: criscar@fmva.unesp.br. FAU - Ambar, Guilherme AU - Ambar G AD - Universidade Estadual Paulista (UNESP), Dept. Zoologia, Instituto de Biociências, Rio Claro, SP, Brazil. Electronic address: guilhermeambar@gmail.com. FAU - Queiroz, Luzia Helena AU - Queiroz LH AD - Universidade Estadual Paulista (UNESP), Dept. de Apoio, Produção e Saúde Animal, Faculdade de Medicina Veterinária de Araçatuba, Araçatuba, SP, Brazil. Electronic address: lhqueiroz@fmva.unesp.br. FAU - Cruz-Neto, Ariovaldo Pereira AU - Cruz-Neto AP AD - Universidade Estadual Paulista (UNESP), Dept. Zoologia, Instituto de Biociências, Rio Claro, SP, Brazil. Electronic address: ariovaldopcruz@gmail.com. FAU - Munir, Muhammad AU - Munir M AD - The Pirbright Institute, Woking, Surrey, United Kingdom. Electronic address: muhammad.munir@pirbright.ac.uk. FAU - Durigon, Edison Luiz AU - Durigon EL AD - Universidade de São Paulo (USP), Dept. Microbiologia, Instituto de Ciências Biomédicas (ICB), Sao Paulo, SP, Brazil. Electronic address: eldurigo@usp.br. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160726 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Brazil MH - Chiroptera/*virology MH - Coronavirus/*classification/*genetics MH - Female MH - *Forests MH - *Genetic Variation MH - Genome, Viral MH - Genotype MH - Male MH - Phylogeny MH - Phylogeography MH - RNA, Viral PMC - PMC7106056 EDAT- 2016/07/31 06:00 MHDA- 2017/09/28 06:00 PMCR- 2016/07/26 CRDT- 2016/07/31 06:00 PHST- 2016/05/03 00:00 [received] PHST- 2016/07/22 00:00 [revised] PHST- 2016/07/25 00:00 [accepted] PHST- 2016/07/31 06:00 [entrez] PHST- 2016/07/31 06:00 [pubmed] PHST- 2017/09/28 06:00 [medline] PHST- 2016/07/26 00:00 [pmc-release] AID - S1567-1348(16)30324-0 [pii] AID - 10.1016/j.meegid.2016.07.034 [doi] PST - ppublish SO - Infect Genet Evol. 2016 Oct;44:510-513. doi: 10.1016/j.meegid.2016.07.034. Epub 2016 Jul 26. PMID- 26916286 OWN - NLM STAT- MEDLINE DCOM- 20161013 LR - 20240324 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 13 DP - 2016 Feb 25 TI - Molecular epidemiology and evolutionary histories of human coronavirus OC43 and HKU1 among patients with upper respiratory tract infections in Kuala Lumpur, Malaysia. PG - 33 LID - 10.1186/s12985-016-0488-4 [doi] LID - 33 AB - BACKGROUND: Despite the worldwide circulation of human coronavirus OC43 (HCoV-OC43) and HKU1 (HCoV-HKU1), data on their molecular epidemiology and evolutionary dynamics in the tropical Southeast Asia region is lacking. METHODS: The study aimed to investigate the genetic diversity, temporal distribution, population history and clinical symptoms of betacoronavirus infections in Kuala Lumpur, Malaysia between 2012 and 2013. A total of 2,060 adults presented with acute respiratory symptoms were screened for the presence of betacoronaviruses using multiplex PCR. The spike glycoprotein, nucleocapsid and 1a genes were sequenced for phylogenetic reconstruction and Bayesian coalescent inference. RESULTS: A total of 48/2060 (2.4 %) specimens were tested positive for HCoV-OC43 (1.3 %) and HCoV-HKU1 (1.1 %). Both HCoV-OC43 and HCoV-HKU1 were co-circulating throughout the year, with the lowest detection rates reported in the October-January period. Phylogenetic analysis of the spike gene showed that the majority of HCoV-OC43 isolates were grouped into two previously undefined genotypes, provisionally assigned as novel lineage 1 and novel lineage 2. Sign of natural recombination was observed in these potentially novel lineages. Location mapping showed that the novel lineage 1 is currently circulating in Malaysia, Thailand, Japan and China, while novel lineage 2 can be found in Malaysia and China. Molecular dating showed the origin of HCoV-OC43 around late 1950s, before it diverged into genotypes A (1960s), B (1990s), and other genotypes (2000s). Phylogenetic analysis revealed that 27.3 % of the HCoV-HKU1 strains belong to genotype A while 72.7 % belongs to genotype B. The tree root of HCoV-HKU1 was similar to that of HCoV-OC43, with the tMRCA of genotypes A and B estimated around the 1990s and 2000s, respectively. Correlation of HCoV-OC43 and HCoV-HKU1 with the severity of respiratory symptoms was not observed. CONCLUSIONS: The present study reported the molecular complexity and evolutionary dynamics of human betacoronaviruses among adults with acute respiratory symptoms in a tropical country. Two novel HCoV-OC43 genetic lineages were identified, warranting further investigation on their genotypic and phenotypic characteristics. FAU - Al-Khannaq, Maryam Nabiel AU - Al-Khannaq MN AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. maryasala@gmail.com. FAU - Ng, Kim Tien AU - Ng KT AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. kimtien@siswa.um.edu.my. FAU - Oong, Xiang Yong AU - Oong XY AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. oxy123@siswa.um.edu.my. FAU - Pang, Yong Kek AU - Pang YK AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. ykpang@ummc.edu.my. FAU - Takebe, Yutaka AU - Takebe Y AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. takebe@niid.go.jp. AD - AIDS Research Center, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, Japan. takebe@niid.go.jp. AD - School of Medicine, Yokohama City University, Yokohama, Kanagawa, Japan. takebe@niid.go.jp. FAU - Chook, Jack Bee AU - Chook JB AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. jackbee2002@um.edu.my. FAU - Hanafi, Nik Sherina AU - Hanafi NS AD - Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. sherina@ummc.edu.my. FAU - Kamarulzaman, Adeeba AU - Kamarulzaman A AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. adeeba@ummc.edu.my. FAU - Tee, Kok Keng AU - Tee KK AD - Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. k2tee@um.edu.my. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160225 PL - England TA - Virol J JT - Virology journal JID - 101231645 RN - 0 (RNA, Viral) SB - IM MH - Adult MH - Aged MH - Coronavirus Infections/diagnosis/*epidemiology/*virology MH - Coronavirus OC43, Human/classification/*genetics/isolation & purification MH - *Evolution, Molecular MH - Female MH - Genes, Viral MH - *Genetic Variation MH - Genotype MH - Humans MH - Malaysia/epidemiology MH - Male MH - Middle Aged MH - Nasopharynx/virology MH - Phylogeny MH - Population Surveillance MH - RNA, Viral MH - Respiratory Tract Infections/diagnosis/*epidemiology/*virology MH - Young Adult PMC - PMC4766700 EDAT- 2016/02/27 06:00 MHDA- 2016/10/14 06:00 PMCR- 2016/02/25 CRDT- 2016/02/27 06:00 PHST- 2015/11/26 00:00 [received] PHST- 2016/02/11 00:00 [accepted] PHST- 2016/02/27 06:00 [entrez] PHST- 2016/02/27 06:00 [pubmed] PHST- 2016/10/14 06:00 [medline] PHST- 2016/02/25 00:00 [pmc-release] AID - 10.1186/s12985-016-0488-4 [pii] AID - 488 [pii] AID - 10.1186/s12985-016-0488-4 [doi] PST - epublish SO - Virol J. 2016 Feb 25;13:33. doi: 10.1186/s12985-016-0488-4. PMID- 26221765 OWN - NLM STAT- MEDLINE DCOM- 20160817 LR - 20200407 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 210 DP - 2015 Dec 2 TI - Full-length genome analysis of canine coronavirus type I. PG - 100-5 LID - S0168-1702(15)30022-8 [pii] LID - 10.1016/j.virusres.2015.07.018 [doi] AB - Canine coronavirus types I (CCoV-I) and II (CCoV-II) are usually responsible for mild enteritis in dogs. While the CCoV-II genome has been completely sequenced, to date there are no complete genomic sequence data available publicly for CCoV-I. Thus, the aim of the present study was to analyze the full-length genome of a CCoV-I prototype strain that had been recovered from a dog with diarrhea in Italy. CCoV-I strain 23/03 has a genome of 30,000 nucleotides, excluding the 3' poly(A) tail, displaying the typical Alphacoronavirus-1 organization and the highest genetic relatedness to CCoV-II. However, two distinct features were observed in the CCoV-I genome: (i) the presence of an additional ORF between the spike (S) protein gene and ORF3a; (ii) the diversity of the S protein, which is more closely related to that of feline coronavirus type I and presents a furin cleavage site. The present study may contribute to a better understanding of the Alphacoronavirus-1 evolutionary pattern and may be paradigmatic of how coronaviruses evolve through gene losses, acquisition and exchanges among different members. CI - Copyright © 2015 Elsevier B.V. All rights reserved. FAU - Decaro, Nicola AU - Decaro N AD - Department of Veterinary Medicine, University of Bari, Valenzano, Italy. Electronic address: nicola.decaro@uniba.it. FAU - Mari, Viviana AU - Mari V AD - Department of Veterinary Medicine, University of Bari, Valenzano, Italy. FAU - Elia, Gabriella AU - Elia G AD - Department of Veterinary Medicine, University of Bari, Valenzano, Italy. FAU - Lanave, Gianvito AU - Lanave G AD - Department of Veterinary Medicine, University of Bari, Valenzano, Italy. FAU - Dowgier, Giulia AU - Dowgier G AD - Department of Veterinary Medicine, University of Bari, Valenzano, Italy. FAU - Colaianni, Maria Loredana AU - Colaianni ML AD - Department of Veterinary Medicine, University of Bari, Valenzano, Italy. FAU - Martella, Vito AU - Martella V AD - Department of Veterinary Medicine, University of Bari, Valenzano, Italy. FAU - Buonavoglia, Canio AU - Buonavoglia C AD - Department of Veterinary Medicine, University of Bari, Valenzano, Italy. LA - eng PT - Journal Article DEP - 20150726 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Cluster Analysis MH - Coronavirus, Canine/*genetics/isolation & purification MH - Diarrhea/virology MH - Dog Diseases/virology MH - Dogs MH - Gene Order MH - Genes, Viral MH - *Genome, Viral MH - Italy MH - Molecular Sequence Data MH - Phylogeny MH - RNA Viruses/*genetics/isolation & purification MH - RNA, Viral/*genetics MH - *Sequence Analysis, DNA MH - Sequence Homology PMC - PMC7114546 OTO - NOTNLM OT - Canine coronavirus type I OT - Dog OT - Genomic analysis EDAT- 2015/07/30 06:00 MHDA- 2016/08/18 06:00 PMCR- 2015/07/26 CRDT- 2015/07/30 06:00 PHST- 2015/05/12 00:00 [received] PHST- 2015/07/09 00:00 [revised] PHST- 2015/07/20 00:00 [accepted] PHST- 2015/07/30 06:00 [entrez] PHST- 2015/07/30 06:00 [pubmed] PHST- 2016/08/18 06:00 [medline] PHST- 2015/07/26 00:00 [pmc-release] AID - S0168-1702(15)30022-8 [pii] AID - 10.1016/j.virusres.2015.07.018 [doi] PST - ppublish SO - Virus Res. 2015 Dec 2;210:100-5. doi: 10.1016/j.virusres.2015.07.018. Epub 2015 Jul 26. PMID- 27128350 OWN - NLM STAT- MEDLINE DCOM- 20170504 LR - 20200407 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 494 DP - 2016 Jul TI - Structural basis for the dimerization and substrate recognition specificity of porcine epidemic diarrhea virus 3C-like protease. PG - 225-35 LID - S0042-6822(16)30076-9 [pii] LID - 10.1016/j.virol.2016.04.018 [doi] AB - Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, has caused significant damage to the Asian and American pork industries. Coronavirus 3C-like protease (3CL(pro)), which is involved in the processing of viral polyproteins for viral replication, is an appealing antiviral drug target. Here, we present the crystal structures of PEDV 3CL(pro) and a molecular complex between an inactive PEDV 3CL(pro) variant C144A bound to a peptide substrate. Structural characterization, mutagenesis and biochemical analysis reveal the substrate-binding pockets and the residues that comprise the active site of PEDV 3CL(pro). The dimerization of PEDV 3CL(pro) is similar to that of other Alphacoronavirus 3CL(pro)s but has several differences from that of SARS-CoV 3CL(pro) from the genus Betacoronavirus. Furthermore, the non-conserved motifs in the pockets cause different cleavage of substrate between PEDV and SARS-CoV 3CL(pro)s, which may provide new insights into the recognition of substrates by 3CL(pro)s in various coronavirus genera. CI - Copyright © 2016 Elsevier Inc. All rights reserved. FAU - Ye, Gang AU - Ye G AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Deng, Feng AU - Deng F AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Shen, Zhou AU - Shen Z AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Luo, Rui AU - Luo R AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Zhao, Ling AU - Zhao L AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Xiao, Shaobo AU - Xiao S AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Fu, Zhen F AU - Fu ZF AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. FAU - Peng, Guiqing AU - Peng G AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China. Electronic address: penggq@mail.hzau.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160426 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Viral Proteins) RN - EC 3.4.- (Peptide Hydrolases) SB - IM MH - Amino Acid Motifs MH - Amino Acid Sequence MH - Animals MH - Binding Sites MH - Catalysis MH - Catalytic Domain MH - Enzyme Activation MH - *Models, Molecular MH - Peptide Hydrolases/*chemistry/metabolism MH - Porcine epidemic diarrhea virus/*enzymology MH - Protein Binding MH - Protein Conformation MH - Protein Interaction Domains and Motifs MH - *Protein Multimerization MH - Structure-Activity Relationship MH - Substrate Specificity MH - Swine MH - Viral Proteins/*chemistry/metabolism PMC - PMC7111274 OTO - NOTNLM OT - Corona virus OT - Crystal structure OT - Dimerization OT - PEDV 3CL(pro) OT - Substrate specificity EDAT- 2016/04/30 06:00 MHDA- 2017/05/05 06:00 PMCR- 2016/04/26 CRDT- 2016/04/30 06:00 PHST- 2015/10/19 00:00 [received] PHST- 2016/04/14 00:00 [revised] PHST- 2016/04/15 00:00 [accepted] PHST- 2016/04/30 06:00 [entrez] PHST- 2016/04/30 06:00 [pubmed] PHST- 2017/05/05 06:00 [medline] PHST- 2016/04/26 00:00 [pmc-release] AID - S0042-6822(16)30076-9 [pii] AID - 10.1016/j.virol.2016.04.018 [doi] PST - ppublish SO - Virology. 2016 Jul;494:225-35. doi: 10.1016/j.virol.2016.04.018. Epub 2016 Apr 26. PMID- 28418192 OWN - NLM STAT- MEDLINE DCOM- 20180808 LR - 20231113 IS - 1863-2378 (Electronic) IS - 1863-1959 (Print) IS - 1863-1959 (Linking) VI - 65 IP - 1 DP - 2018 Feb TI - Detection of potentially novel paramyxovirus and coronavirus viral RNA in bats and rats in the Mekong Delta region of southern Viet Nam. PG - 30-42 LID - 10.1111/zph.12362 [doi] AB - Bats and rodents are being increasingly recognized as reservoirs of emerging zoonotic viruses. Various studies have investigated bat viruses in tropical regions, but to date there are no data regarding viruses with zoonotic potential that circulate in bat and rat populations in Viet Nam. To address this paucity of data, we sampled three bat farms and three wet markets trading in rat meat in the Mekong Delta region of southern Viet Nam. Faecal and urine samples were screened for the presence of RNA from paramyxoviruses, coronaviruses and filoviruses. Paramyxovirus RNA was detected in 4 of 248 (1%) and 11 of 222 (4.9%) bat faecal and urine samples, respectively. Coronavirus RNA was detected in 55 of 248 (22%) of bat faecal samples; filovirus RNA was not detected in any of the bat samples. Further, coronavirus RNA was detected in 12 of 270 (4.4%) of rat faecal samples; all samples tested negative for paramyxovirus. Phylogenetic analysis revealed that the bat paramyxoviruses and bat and rat coronaviruses were related to viruses circulating in bat and rodent populations globally, but showed no cross-species mixing of viruses between bat and rat populations within Viet Nam. Our study shows that potentially novel variants of paramyxoviruses and coronaviruses commonly circulate in bat and rat populations in Viet Nam. Further characterization of the viruses and additional human and animal surveillance is required to evaluate the likelihood of viral spillover and to assess whether these viruses pose a risk to human health. CI - © 2017 The Authors. Zoonoses and Public Health Published by Blackwell Verlag GmbH. FAU - Berto, A AU - Berto A AUID- ORCID: 0000-0003-4005-9464 AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. FAU - Anh, P H AU - Anh PH AUID- ORCID: 0000-0001-9668-9233 AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. FAU - Carrique-Mas, J J AU - Carrique-Mas JJ AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. AD - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK. FAU - Simmonds, P AU - Simmonds P AD - Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK. FAU - Van Cuong, N AU - Van Cuong N AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. FAU - Tue, N T AU - Tue NT AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. FAU - Van Dung, N AU - Van Dung N AD - Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK. FAU - Woolhouse, M E AU - Woolhouse ME AD - Centre for Immunity, Infection & Evolution, The University of Edinburgh, Edinburgh, UK. FAU - Smith, I AU - Smith I AD - Health and Biosecurity, CSIRO, Australian Animal Health Laboratory, Geelong, Vic., Australia. FAU - Marsh, G A AU - Marsh GA AD - Health and Biosecurity, CSIRO, Australian Animal Health Laboratory, Geelong, Vic., Australia. FAU - Bryant, J E AU - Bryant JE AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. AD - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK. FAU - Thwaites, G E AU - Thwaites GE AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. AD - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK. FAU - Baker, S AU - Baker S AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. AD - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK. AD - The London School of Hygiene and Tropical Medicine, London, UK. FAU - Rabaa, M A AU - Rabaa MA AUID- ORCID: 0000-0003-0529-2228 AD - Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. AD - Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, Oxford University, Oxford, UK. CN - VIZIONS consortium LA - eng SI - GENBANK/KX092148 SI - GENBANK/KX092159 SI - GENBANK/KX092163 SI - GENBANK/KX092228 GR - WT/093724/WT_/Wellcome Trust/United Kingdom GR - MC_UU_12014/12/MRC_/Medical Research Council/United Kingdom GR - 110085/Z/15/Z/Wellcome Trust/United Kingdom GR - Wellcome Trust/United Kingdom GR - 100087/Z/12/Z/WT_/Wellcome Trust/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170418 PL - Germany TA - Zoonoses Public Health JT - Zoonoses and public health JID - 101300786 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Chiroptera/virology MH - Coronavirus/*genetics/isolation & purification MH - Feces/virology MH - Filoviridae/isolation & purification MH - Humans MH - Paramyxoviridae/*genetics/isolation & purification MH - Phylogeny MH - RNA, Viral/isolation & purification MH - Rats MH - Urine/virology MH - Vietnam PMC - PMC5811810 OTO - NOTNLM OT - Viet Nam OT - bats OT - coronavirus OT - paramyxovirus OT - rats OT - zoonotic viruses FIR - Kiet, Bach Tuan IR - Kiet BT FIR - Boni, Maciej F IR - Boni MF FIR - Phu, Bui Duc IR - Phu BD FIR - Campbell, James I IR - Campbell JI FIR - Hung, Dang Manh IR - Hung DM FIR - Huong, Dang Thao IR - Huong DT FIR - Oanh, Dang Tram IR - Oanh DT FIR - Day, Jeremy N IR - Day JN FIR - Van Tan, Dinh IR - Van Tan D FIR - van Doorn, H Rogier IR - van Doorn HR FIR - Han, Duong An IR - Han DA FIR - Farrar, Jeremy J IR - Farrar JJ FIR - Trang, Hau Thi Thu IR - Trang HTT FIR - Nghia, Ho Dang Trung IR - Nghia HDT FIR - Long, Hoang Bao IR - Long HB FIR - Van Duong, Hoang IR - Van Duong H FIR - Thu, Huynh Thi Kim IR - Thu HTK FIR - Cuong, Lam Chi IR - Cuong LC FIR - Hung, Manh IR - Hung M FIR - Phuong, Thanh IR - Phuong T FIR - Phuc, Thi IR - Phuc T FIR - Phuong, Thi IR - Phuong T FIR - Luat, Xuan IR - Luat X FIR - Ha, Luu Thi Thu IR - Ha LTT FIR - Van Chuong, Ly IR - Van Chuong L FIR - Loan, Mai Thi Phuoc IR - Loan MTP FIR - Nadjm, Behzad IR - Nadjm B FIR - Bao, Ngo Thanh IR - Bao NT FIR - Tu, Nguyen Canh IR - Tu NC FIR - Thuan, Nguyen Dac IR - Thuan ND FIR - Dong, Nguyen IR - Dong N FIR - Chuyen, Nguyen Khac IR - Chuyen NK FIR - An, Nguyen Ngoc IR - An NN FIR - Vinh, Nguyen Ngoc IR - Vinh NN FIR - Hung, Nguyen Quoc IR - Hung NQ FIR - Dung, Nguyen Thanh IR - Dung NT FIR - Minh, Nguyen Thanh IR - Minh NT FIR - Binh, Nguyen Thi IR - Binh NT FIR - Tham, Nguyen Thi Hong IR - Tham NTH FIR - Tien, Nguyen Thi Hong IR - Tien NTH FIR - Chuc, Nguyen Thi Kim IR - Chuc NTK FIR - Le Ngoc, Nguyen Thi IR - Le Ngoc NT FIR - Ha, Nguyen Thi Lien IR - Ha NTL FIR - Lien, Nguyen Thi Nam IR - Lien NTN FIR - Diep, Nguyen Thi Ngoc IR - Diep NTN FIR - Nhung, Nguyen Thi IR - Nhung NT FIR - Chau, Nguyen Thi Song IR - Chau NTS FIR - Chi, Nguyen Thi Yen IR - Chi NTY FIR - Trinh, Nguyen Thieu IR - Trinh NT FIR - Van, Nguyen Thu IR - Van NT FIR - Van Hung, Nguyen IR - Van Hung N FIR - Van Kinh, Nguyen IR - Van Kinh N FIR - Van Minh Hoang, Nguyen IR - Van Minh Hoang N FIR - Van My, Nguyen IR - Van My N FIR - Van Thang, Nguyen IR - Van Thang N FIR - Van Thanh, Nguyen IR - Van Thanh N FIR - Van Vinh Chau, Nguyen IR - Van Vinh Chau N FIR - Van Xang, Nguyen IR - Van Xang N FIR - My, Pham Ha IR - My PH FIR - Khoa, Pham Thi Minh IR - Khoa PTM FIR - Tam, Pham Thi Thanh IR - Tam PTT FIR - Van Lao, Pham IR - Van Lao P FIR - Van Minh, Pham IR - Van Minh P FIR - Van Be Bay, Phan IR - Van Be Bay P FIR - Rahman, Motiur IR - Rahman M FIR - Thompson, Corinne IR - Thompson C FIR - Ngan, Ta Thi Dieu IR - Ngan TTD FIR - Nhu, Tran Do Hoang IR - Nhu TDH FIR - Chau, Tran Hoang Minh IR - Chau THM FIR - Toan, Tran Khanh IR - Toan TK FIR - Phuc, Tran My IR - Phuc TM FIR - Hong, Tran Thi Kim IR - Hong TTK FIR - Dung, Tran Thi Ngoc IR - Dung TTN FIR - Thanh, Tran Thi Thanh IR - Thanh TTT FIR - Minh, Tran Thi Thuy IR - Minh TTT FIR - Nguyen, Tran Thua IR - Nguyen TT FIR - Hien, Tran Tinh IR - Hien TT FIR - Tri, Trinh Quang IR - Tri TQ FIR - Hien, Vo Be IR - Hien VB FIR - Tai, Vo Nhut IR - Tai VN FIR - Cuong, Vo Quoc IR - Cuong VQ FIR - Phat, Voong Vinh IR - Phat VV FIR - Huong, Vu Thi Lan IR - Huong VTL FIR - Hang, Vu Thi Ty IR - Hang VTT FIR - Wertheim, Heiman IR - Wertheim H FIR - Bogaardt, Carlijn IR - Bogaardt C FIR - Brierley, Liam IR - Brierley L FIR - Chase-Topping, Margo IR - Chase-Topping M FIR - Ivens, Al IR - Ivens A FIR - Lu, Lu IR - Lu L FIR - Rambaut, Andrew IR - Rambaut A FIR - Woolhouse, Mark IR - Woolhouse M FIR - Cotten, Matthew IR - Cotten M FIR - Oude Munnink, Bas B IR - Oude Munnink BB FIR - Kellam, Paul IR - Kellam P FIR - Phan, My Vu Tra IR - Phan MVT FIR - van der Hoek, Lia IR - van der Hoek L FIR - Deijs, Martin IR - Deijs M FIR - Jebbink, Maarten F IR - Jebbink MF FIR - Farsani, Seyed Mohammad Jazaeri IR - Farsani SMJ FIR - Saylors, Karen IR - Saylors K FIR - Wolfe, Nathan IR - Wolfe N EDAT- 2017/04/19 06:00 MHDA- 2018/08/09 06:00 PMCR- 2020/04/18 CRDT- 2017/04/19 06:00 PHST- 2016/06/06 00:00 [received] PHST- 2017/04/19 06:00 [pubmed] PHST- 2018/08/09 06:00 [medline] PHST- 2017/04/19 06:00 [entrez] PHST- 2020/04/18 00:00 [pmc-release] AID - ZPH12362 [pii] AID - 10.1111/zph.12362 [doi] PST - ppublish SO - Zoonoses Public Health. 2018 Feb;65(1):30-42. doi: 10.1111/zph.12362. Epub 2017 Apr 18. PMID- 28659436 OWN - NLM STAT- MEDLINE DCOM- 20180330 LR - 20201209 IS - 1946-6242 (Electronic) IS - 1946-6234 (Print) IS - 1946-6234 (Linking) VI - 9 IP - 396 DP - 2017 Jun 28 TI - Broad-spectrum antiviral GS-5734 inhibits both epidemic and zoonotic coronaviruses. LID - 10.1126/scitranslmed.aal3653 [doi] LID - eaal3653 AB - Emerging viral infections are difficult to control because heterogeneous members periodically cycle in and out of humans and zoonotic hosts, complicating the development of specific antiviral therapies and vaccines. Coronaviruses (CoVs) have a proclivity to spread rapidly into new host species causing severe disease. Severe acute respiratory syndrome CoV (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV) successively emerged, causing severe epidemic respiratory disease in immunologically naïve human populations throughout the globe. Broad-spectrum therapies capable of inhibiting CoV infections would address an immediate unmet medical need and could be invaluable in the treatment of emerging and endemic CoV infections. We show that a nucleotide prodrug, GS-5734, currently in clinical development for treatment of Ebola virus disease, can inhibit SARS-CoV and MERS-CoV replication in multiple in vitro systems, including primary human airway epithelial cell cultures with submicromolar IC(50) values. GS-5734 was also effective against bat CoVs, prepandemic bat CoVs, and circulating contemporary human CoV in primary human lung cells, thus demonstrating broad-spectrum anti-CoV activity. In a mouse model of SARS-CoV pathogenesis, prophylactic and early therapeutic administration of GS-5734 significantly reduced lung viral load and improved clinical signs of disease as well as respiratory function. These data provide substantive evidence that GS-5734 may prove effective against endemic MERS-CoV in the Middle East, circulating human CoV, and, possibly most importantly, emerging CoV of the future. CI - Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. FAU - Sheahan, Timothy P AU - Sheahan TP AUID- ORCID: 0000-0001-9181-2183 AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. FAU - Sims, Amy C AU - Sims AC AUID- ORCID: 0000-0003-0178-4225 AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. FAU - Graham, Rachel L AU - Graham RL AUID- ORCID: 0000-0002-3143-6515 AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. FAU - Menachery, Vineet D AU - Menachery VD AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. FAU - Gralinski, Lisa E AU - Gralinski LE AUID- ORCID: 0000-0003-1374-8002 AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. FAU - Case, James B AU - Case JB AD - Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA. FAU - Leist, Sarah R AU - Leist SR AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. FAU - Pyrc, Krzysztof AU - Pyrc K AUID- ORCID: 0000-0002-3867-7688 AD - Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Feng, Joy Y AU - Feng JY AUID- ORCID: 0000-0003-4837-1911 AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Trantcheva, Iva AU - Trantcheva I AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Bannister, Roy AU - Bannister R AUID- ORCID: 0000-0002-6311-1440 AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Park, Yeojin AU - Park Y AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Babusis, Darius AU - Babusis D AUID- ORCID: 0000-0001-5599-1671 AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Clarke, Michael O AU - Clarke MO AUID- ORCID: 0000-0002-7266-1364 AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Mackman, Richard L AU - Mackman RL AUID- ORCID: 0000-0001-8861-7205 AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Spahn, Jamie E AU - Spahn JE AUID- ORCID: 0000-0003-2110-5824 AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Palmiotti, Christopher A AU - Palmiotti CA AUID- ORCID: 0000-0001-9367-2487 AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Siegel, Dustin AU - Siegel D AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Ray, Adrian S AU - Ray AS AUID- ORCID: 0000-0002-3508-3008 AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Cihlar, Tomas AU - Cihlar T AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Jordan, Robert AU - Jordan R AD - Gilead Sciences Inc., Foster City, CA 94404, USA. FAU - Denison, Mark R AU - Denison MR AD - Division of Infectious Diseases, Department of Pediatrics and Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA. mark.denison@vanderbilt.edu rbaric@email.unc.edu. FAU - Baric, Ralph S AU - Baric RS AUID- ORCID: 0000-0001-6827-8701 AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. mark.denison@vanderbilt.edu rbaric@email.unc.edu. LA - eng GR - U19 AI109761/AI/NIAID NIH HHS/United States GR - R01 AI108197/AI/NIAID NIH HHS/United States GR - R01 AI132178/AI/NIAID NIH HHS/United States GR - P30 DK065988/DK/NIDDK NIH HHS/United States GR - U19 AI109680/AI/NIAID NIH HHS/United States PT - Journal Article PL - United States TA - Sci Transl Med JT - Science translational medicine JID - 101505086 RN - 0 (Antiviral Agents) RN - 0 (Ribonucleotides) RN - 3QKI37EEHE (remdesivir) RN - 415SHH325A (Adenosine Monophosphate) RN - OF5P57N2ZX (Alanine) SB - IM MH - Adenosine Monophosphate/analogs & derivatives MH - Alanine/*analogs & derivatives/metabolism/pharmacokinetics/pharmacology/toxicity MH - Animals MH - Antiviral Agents/metabolism/pharmacokinetics/*pharmacology/toxicity MH - Callithrix MH - Cell Line MH - Coronavirus/*drug effects MH - *Epidemics MH - Epithelial Cells/virology MH - Humans MH - Lung/pathology MH - Mice MH - Ribonucleotides/metabolism/pharmacokinetics/*pharmacology/toxicity MH - Virus Replication/drug effects MH - Zoonoses/*epidemiology/prevention & control/*virology PMC - PMC5567817 MID - NIHMS895143 COIS- Competing interests: The authors affiliated with Gilead Sciences are employees of the company and may own company stock. M.O.C., J.Y.F, R.J., R.L.M., A.S.R., and D.S. are listed as inventors on International Application No. PCT/US2016/052092 filed by Gilead Sciences, Inc., directed to methods of treating coronaviridae virus infections. Travel of M.R.D. to Gilead Sciences, Inc. to discuss this project was paid for by Gilead Sciences. EDAT- 2017/07/01 06:00 MHDA- 2018/03/31 06:00 PMCR- 2017/08/23 CRDT- 2017/06/30 06:00 PHST- 2016/11/08 00:00 [received] PHST- 2017/05/17 00:00 [accepted] PHST- 2017/06/30 06:00 [entrez] PHST- 2017/07/01 06:00 [pubmed] PHST- 2018/03/31 06:00 [medline] PHST- 2017/08/23 00:00 [pmc-release] AID - 9/396/eaal3653 [pii] AID - 10.1126/scitranslmed.aal3653 [doi] PST - ppublish SO - Sci Transl Med. 2017 Jun 28;9(396):eaal3653. doi: 10.1126/scitranslmed.aal3653. PMID- 29249267 OWN - NLM STAT- MEDLINE DCOM- 20180420 LR - 20210109 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 517 DP - 2018 Apr TI - Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture. PG - 148-156 LID - S0042-6822(17)30401-4 [pii] LID - 10.1016/j.virol.2017.11.022 [doi] AB - Cyclophilin A (CypA) is an important host factor in the replication of a variety of RNA viruses. Also the replication of several nidoviruses was reported to depend on CypA, although possibly not to the same extent. These prior studies are difficult to compare, since different nidoviruses, cell lines and experimental set-ups were used. Here, we investigated the CypA dependence of three distantly related nidoviruses that can all replicate in Huh7 cells: the arterivirus equine arteritis virus (EAV), the alphacoronavirus human coronavirus 229E (HCoV-229E), and the betacoronavirus Middle East respiratory syndrome coronavirus (MERS-CoV). The replication of these viruses was compared in the same parental Huh7 cells and in CypA-knockout Huh7 cells generated using CRISPR/Cas9-technology. CypA depletion reduced EAV yields by ~ 3-log, whereas MERS-CoV progeny titers were modestly reduced (3-fold) and HCoV-229E replication was unchanged. This study reveals that the replication of nidoviruses can differ strikingly in its dependence on cellular CypA. CI - Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved. FAU - de Wilde, Adriaan H AU - de Wilde AH AD - Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: A.H.de_Wilde@lumc.nl. FAU - Zevenhoven-Dobbe, Jessika C AU - Zevenhoven-Dobbe JC AD - Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. FAU - Beugeling, Corrine AU - Beugeling C AD - Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. FAU - Chatterji, Udayan AU - Chatterji U AD - Department of Immunology & Microbiology, The Scripps Research Institute, La Jolla, CA 92037, United States. FAU - de Jong, Danielle AU - de Jong D AD - Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands. FAU - Gallay, Philippe AU - Gallay P AD - Department of Immunology & Microbiology, The Scripps Research Institute, La Jolla, CA 92037, United States. FAU - Szuhai, Karoly AU - Szuhai K AD - Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands. FAU - Posthuma, Clara C AU - Posthuma CC AD - Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. FAU - Snijder, Eric J AU - Snijder EJ AD - Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: E.J.Snijder@lumc.nl. LA - eng PT - Journal Article DEP - 20171215 PL - United States TA - Virology JT - Virology JID - 0110674 RN - EC 5.2.1.- (Cyclophilin A) SB - IM MH - Animals MH - Arterivirus/*physiology MH - Cell Line MH - Coronavirus/*physiology MH - Cricetinae MH - Cyclophilin A/*metabolism MH - Humans MH - *Virus Cultivation MH - Virus Replication/*physiology PMC - PMC7112125 OTO - NOTNLM OT - Arterivirus OT - CRISPR/Cas9 OT - Cyclophilin OT - CypA OT - EAV OT - Knockout OT - MERS-coronavirus OT - human coronavirus-229E EDAT- 2017/12/19 06:00 MHDA- 2018/04/21 06:00 PMCR- 2017/12/15 CRDT- 2017/12/19 06:00 PHST- 2017/10/20 00:00 [received] PHST- 2017/11/27 00:00 [revised] PHST- 2017/11/28 00:00 [accepted] PHST- 2017/12/19 06:00 [pubmed] PHST- 2018/04/21 06:00 [medline] PHST- 2017/12/19 06:00 [entrez] PHST- 2017/12/15 00:00 [pmc-release] AID - S0042-6822(17)30401-4 [pii] AID - 10.1016/j.virol.2017.11.022 [doi] PST - ppublish SO - Virology. 2018 Apr;517:148-156. doi: 10.1016/j.virol.2017.11.022. Epub 2017 Dec 15. PMID- 25653111 OWN - NLM STAT- MEDLINE DCOM- 20160927 LR - 20240328 IS - 1863-2378 (Electronic) IS - 1863-1959 (Print) IS - 1863-1959 (Linking) VI - 62 IP - 7 DP - 2015 Nov TI - Neotropical Bats from Costa Rica harbour Diverse Coronaviruses. PG - 501-5 LID - 10.1111/zph.12181 [doi] AB - Bats are hosts of diverse coronaviruses (CoVs) known to potentially cross the host-species barrier. For analysing coronavirus diversity in a bat species-rich country, a total of 421 anal swabs/faecal samples from Costa Rican bats were screened for CoV RNA-dependent RNA polymerase (RdRp) gene sequences by a pancoronavirus PCR. Six families, 24 genera and 41 species of bats were analysed. The detection rate for CoV was 1%. Individuals (n = 4) from four different species of frugivorous (Artibeus jamaicensis, Carollia perspicillata and Carollia castanea) and nectivorous (Glossophaga soricina) bats were positive for coronavirus-derived nucleic acids. Analysis of 440 nt. RdRp sequences allocated all Costa Rican bat CoVs to the α-CoV group. Several CoVs sequences clustered near previously described CoVs from the same species of bat, but were phylogenetically distant from the human CoV sequences identified to date, suggesting no recent spillover events. The Glossophaga soricina CoV sequence is sufficiently dissimilar (26% homology to the closest known bat CoVs) to represent a unique coronavirus not clustering near other CoVs found in the same bat species so far, implying an even higher CoV diversity than previously suspected. CI - © 2015 Blackwell Verlag GmbH. FAU - Moreira-Soto, A AU - Moreira-Soto A AD - CIET (Research Center for Tropical Diseases), Virology, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica. FAU - Taylor-Castillo, L AU - Taylor-Castillo L AD - CIET (Research Center for Tropical Diseases), Virology, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica. FAU - Vargas-Vargas, N AU - Vargas-Vargas N AD - CIET (Research Center for Tropical Diseases), Virology, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica. FAU - Rodríguez-Herrera, B AU - Rodríguez-Herrera B AD - School of Biology, University of Costa Rica, San José, Costa Rica. FAU - Jiménez, C AU - Jiménez C AD - Tropical Diseases Research Program (PIET), National University of Costa Rica, Heredia, Costa Rica. FAU - Corrales-Aguilar, E AU - Corrales-Aguilar E AD - CIET (Research Center for Tropical Diseases), Virology, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica. LA - eng SI - GENBANK/EU769558 SI - GENBANK/JQ731784 SI - GENBANK/KC779225 SI - GENBANK/KC779226 SI - GENBANK/KM215146 SI - GENBANK/KM215147 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150204 PL - Germany TA - Zoonoses Public Health JT - Zoonoses and public health JID - 101300786 RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Animals MH - Bayes Theorem MH - Chiroptera/classification/*virology MH - Communicable Diseases, Emerging/genetics/veterinary/virology MH - Coronavirus/enzymology/*genetics/isolation & purification MH - Coronavirus Infections/epidemiology/genetics/*veterinary/virology MH - Costa Rica/epidemiology MH - Feces/virology MH - Genetic Variation MH - Genome, Viral MH - Humans MH - Phylogeny MH - RNA-Dependent RNA Polymerase/genetics MH - Reverse Transcriptase Polymerase Chain Reaction MH - Sequence Analysis PMC - PMC7165833 OTO - NOTNLM OT - Bats OT - Coronavirus OT - Costa Rica OT - phylogenetic analysis COIS- The authors disclose no conflict of interest. EDAT- 2015/02/06 06:00 MHDA- 2016/09/28 06:00 PMCR- 2020/04/18 CRDT- 2015/02/06 06:00 PHST- 2014/08/30 00:00 [received] PHST- 2015/02/06 06:00 [entrez] PHST- 2015/02/06 06:00 [pubmed] PHST- 2016/09/28 06:00 [medline] PHST- 2020/04/18 00:00 [pmc-release] AID - ZPH12181 [pii] AID - 10.1111/zph.12181 [doi] PST - ppublish SO - Zoonoses Public Health. 2015 Nov;62(7):501-5. doi: 10.1111/zph.12181. Epub 2015 Feb 4. PMID- 29704627 OWN - NLM STAT- MEDLINE DCOM- 20190530 LR - 20200605 IS - 1567-7257 (Electronic) IS - 1567-1348 (Print) IS - 1567-1348 (Linking) VI - 62 DP - 2018 Aug TI - Newly emerged porcine enteric alphacoronavirus in southern China: Identification, origin and evolutionary history analysis. PG - 179-187 LID - S1567-1348(18)30210-7 [pii] LID - 10.1016/j.meegid.2018.04.031 [doi] AB - Coronaviruses have a wide host range and can cause a variety of diseases with varying severity in different animals. Several enteric coronaviruses have been identified that are associated with diarrhea in swine and that have caused substantial economic losses. In this study, a newly emerged porcine enteric alphacoronavirus (PEAV), PEAV-GD-CH/2017, was identified from suckling piglets with diarrhea in southern China, and a full-length genome sequence of PEAV was obtained for systematic analysis. The novel PEAV sequence was most identical to that of bat-HKU2, and the differences between them were comprehensively compared, especially the uniform features of the S protein, which was shown to have a close relationship with betacoronaviruses and to perhaps represent unrecognized betacoronaviruses. In addition, Bayesian analysis was conducted to address the origin of PEAV, and the divergence time between PEAV and bat-HKU2 was estimated at 1926, which indicates that PEAV is not newly emerged and may have circulated in swine herds for several decades since the interspecies transmission of this coronavirus from bat to swine. The evolutionary rate of coronaviruses was estimated to be 1.93 × 10(-4) substitutions per site per year for the RdRp gene in our analysis. For the origin of PEAV, we suspect that it is the result of the interspecies transmission of bat-HKU2 from bat to swine. Our results provide valuable information about the uniform features, origin and evolution of the novel PEAV, which will facilitate further investigations of this newly emerged pathogen. CI - Copyright © 2018 Elsevier B.V. All rights reserved. FAU - Fu, Xinliang AU - Fu X AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Fang, Bo AU - Fang B AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Liu, Yixing AU - Liu Y AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Cai, Mengkai AU - Cai M AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Jun, Junming AU - Jun J AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Ma, Jun AU - Ma J AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases of Guangdong Province, Guangzhou, China. FAU - Bu, Dexin AU - Bu D AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. FAU - Wang, Lifang AU - Wang L AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases of Guangdong Province, Guangzhou, China. FAU - Zhou, Pei AU - Zhou P AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases of Guangdong Province, Guangzhou, China. FAU - Wang, Heng AU - Wang H AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases of Guangdong Province, Guangzhou, China. Electronic address: wangheng2009@scau.edu.cn. FAU - Zhang, Guihong AU - Zhang G AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China. Electronic address: guihongzh@scau.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180425 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 SB - IM MH - Alphacoronavirus/genetics/*isolation & purification MH - Animals MH - *Biological Evolution MH - China/epidemiology MH - Communicable Diseases, Emerging/epidemiology/*veterinary/virology MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Genome, Viral MH - Phylogeny MH - Swine MH - Swine Diseases/epidemiology/*virology PMC - PMC7106130 OTO - NOTNLM OT - Coronavirus OT - Diarrhea OT - Evolutionary analysis OT - Origin OT - PEAV EDAT- 2018/04/29 06:00 MHDA- 2019/05/31 06:00 PMCR- 2018/04/25 CRDT- 2018/04/29 06:00 PHST- 2018/01/11 00:00 [received] PHST- 2018/04/02 00:00 [revised] PHST- 2018/04/24 00:00 [accepted] PHST- 2018/04/29 06:00 [pubmed] PHST- 2019/05/31 06:00 [medline] PHST- 2018/04/29 06:00 [entrez] PHST- 2018/04/25 00:00 [pmc-release] AID - S1567-1348(18)30210-7 [pii] AID - 10.1016/j.meegid.2018.04.031 [doi] PST - ppublish SO - Infect Genet Evol. 2018 Aug;62:179-187. doi: 10.1016/j.meegid.2018.04.031. Epub 2018 Apr 25. PMID- 28608547 OWN - NLM STAT- MEDLINE DCOM- 20180528 LR - 20200422 IS - 1099-1352 (Electronic) IS - 0952-3499 (Print) IS - 0952-3499 (Linking) VI - 30 IP - 11 DP - 2017 Nov TI - Computational modeling of the bat HKU4 coronavirus 3CL(pro) inhibitors as a tool for the development of antivirals against the emerging Middle East respiratory syndrome (MERS) coronavirus. LID - 10.1002/jmr.2644 [doi] LID - e2644 AB - The Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging virus that poses a major challenge to clinical management. The 3C-like protease (3CL(pro) ) is essential for viral replication and thus represents a potential target for antiviral drug development. Presently, very few data are available on MERS-CoV 3CL(pro) inhibition by small molecules. We conducted extensive exploration of the pharmacophoric space of a recently identified set of peptidomimetic inhibitors of the bat HKU4-CoV 3CL(pro) . HKU4-CoV 3CL(pro) shares high sequence identity (81%) with the MERS-CoV enzyme and thus represents a potential surrogate model for anti-MERS drug discovery. We used 2 well-established methods: Quantitative structure-activity relationship (QSAR)-guided modeling and docking-based comparative intermolecular contacts analysis. The established pharmacophore models highlight structural features needed for ligand recognition and revealed important binding-pocket regions involved in 3CL(pro) -ligand interactions. The best models were used as 3D queries to screen the National Cancer Institute database for novel nonpeptidomimetic 3CL(pro) inhibitors. The identified hits were tested for HKU4-CoV and MERS-CoV 3CL(pro) inhibition. Two hits, which share the phenylsulfonamide fragment, showed moderate inhibitory activity against the MERS-CoV 3CL(pro) and represent a potential starting point for the development of novel anti-MERS agents. To the best of our knowledge, this is the first pharmacophore modeling study supported by in vitro validation on the MERS-CoV 3CL(pro) . HIGHLIGHTS: MERS-CoV is an emerging virus that is closely related to the bat HKU4-CoV. 3CL(pro) is a potential drug target for coronavirus infection. HKU4-CoV 3CL(pro) is a useful surrogate model for the identification of MERS-CoV 3CL(pro) enzyme inhibitors. dbCICA is a very robust modeling method for hit identification. The phenylsulfonamide scaffold represents a potential starting point for MERS coronavirus 3CL(pro) inhibitors development. CI - Copyright © 2017 John Wiley & Sons, Ltd. FAU - Abuhammad, Areej AU - Abuhammad A AUID- ORCID: 0000-0003-4978-5059 AD - Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, Jordan. FAU - Al-Aqtash, Rua'a A AU - Al-Aqtash RA AD - Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, Jordan. FAU - Anson, Brandon J AU - Anson BJ AD - Department of Biological Sciences, Purdue University, West Lafayette, IN, USA. FAU - Mesecar, Andrew D AU - Mesecar AD AD - Department of Biological Sciences, Purdue University, West Lafayette, IN, USA. AD - Department of Chemistry, Purdue University, West Lafayette, IN, USA. AD - Centers for Cancer Research & Drug Discovery, Purdue University, West Lafayette, IN, USA. FAU - Taha, Mutasem O AU - Taha MO AUID- ORCID: 0000-0002-4453-072X AD - Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, Jordan. LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20170613 PL - England TA - J Mol Recognit JT - Journal of molecular recognition : JMR JID - 9004580 RN - 0 (Antiviral Agents) RN - 0 (Ligands) RN - 0 (Protease Inhibitors) RN - 0 (Viral Proteins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Antiviral Agents/*pharmacology MH - Betacoronavirus/drug effects/*enzymology MH - Binding Sites MH - Chiroptera/*virology MH - Computer Simulation MH - Ligands MH - Middle East Respiratory Syndrome Coronavirus/*drug effects MH - Models, Molecular MH - Protease Inhibitors/chemistry/*pharmacology MH - Quantitative Structure-Activity Relationship MH - ROC Curve MH - Reproducibility of Results MH - Viral Proteins/*antagonists & inhibitors/chemistry PMC - PMC7166879 OTO - NOTNLM OT - 3CLpro inhibitors OT - MERS OT - coronavirus OT - dbCICA OT - pharmacophore modeling EDAT- 2017/06/14 06:00 MHDA- 2018/05/29 06:00 PMCR- 2020/04/19 CRDT- 2017/06/14 06:00 PHST- 2017/02/18 00:00 [received] PHST- 2017/05/01 00:00 [revised] PHST- 2017/05/09 00:00 [accepted] PHST- 2017/06/14 06:00 [pubmed] PHST- 2018/05/29 06:00 [medline] PHST- 2017/06/14 06:00 [entrez] PHST- 2020/04/19 00:00 [pmc-release] AID - JMR2644 [pii] AID - 10.1002/jmr.2644 [doi] PST - ppublish SO - J Mol Recognit. 2017 Nov;30(11):e2644. doi: 10.1002/jmr.2644. Epub 2017 Jun 13. PMID- 28506792 OWN - NLM STAT- MEDLINE DCOM- 20180222 LR - 20200407 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 237 DP - 2017 Jun 2 TI - Discovery of a novel canine respiratory coronavirus support genetic recombination among betacoronavirus1. PG - 7-13 LID - S0168-1702(17)30233-2 [pii] LID - 10.1016/j.virusres.2017.05.006 [doi] AB - Although canine respiratory coronavirus (CRCoV) is an important respiratory pathogen that is prevalent in many countries, only one complete genome sequence of CRCoV (South Korea strain K37) has been obtained to date. Genome-wide analyses and recombination have rarely been conducted, as small numbers of samples and limited genomic characterization have previously prevented further analyses. Herein, we report a unique CRCoV strain, denoted strain BJ232, derived from a CRCoV-positive dog with a mild respiratory infection. Phylogenetic analysis based on complete genome of all available coronaviruses consistently show that CRCoV BJ232 is most closely related to human coronavirus OC43 (HCoV-OC43) and BCoV, forming a separate clade that split off early from other Betacoronavirus 1. Based on the phylogenetic and SimPlot analysis we propose that CRCoV-K37 was derived from genetic recombination between CRCoV-BJ232 and BCoV. In detail, spike (S) gene of CRCoV-K37 clustered with CRCoV-BJ232. However orf1ab, membrane (M) and nucleocapsid (N) genes were more related to Bovine coronavirus (BCoV) than CRCoV-B232. Molecular epidemic analysis confirmed the prevalence of CRCoV-BJ232 lineage around the world for a long time. Recombinant events among Betacoronavirus 1 may have implications for CRCoV transmissibility. All these findings provide further information regarding the origin of CRCoV. CI - Copyright © 2017. Published by Elsevier B.V. FAU - Lu, Shuai AU - Lu S AD - Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325000, China; Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. FAU - Wang, Yanqun AU - Wang Y AD - Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China; State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China. FAU - Chen, Yingzhu AU - Chen Y AD - Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325000, China. FAU - Wu, Bingjie AU - Wu B AD - Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325000, China. FAU - Qin, Kun AU - Qin K AD - Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. FAU - Zhao, Jincun AU - Zhao J AD - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China. FAU - Lou, Yongliang AU - Lou Y AD - Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325000, China. FAU - Tan, Wenjie AU - Tan W AD - Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325000, China; Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China. Electronic address: tanwj28@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170513 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (RNA, Viral) RN - 0 (Viral Proteins) SB - IM MH - Animals MH - Betacoronavirus 1/*genetics MH - Coronavirus Infections/veterinary/virology MH - Coronavirus, Canine/classification/*genetics/*isolation & purification MH - Dog Diseases/virology MH - Dogs MH - Korea MH - Molecular Epidemiology MH - Phylogeny MH - RNA, Viral/genetics MH - *Recombination, Genetic MH - Respiratory Tract Infections/veterinary/virology MH - Sequence Analysis, DNA MH - Viral Proteins/genetics MH - Whole Genome Sequencing PMC - PMC7114567 OTO - NOTNLM OT - Betacoronavirus OT - Canine respiratory coronavirus OT - Genome OT - Origin OT - Phylogenetic analysis OT - Recombination EDAT- 2017/05/17 06:00 MHDA- 2018/02/23 06:00 PMCR- 2017/05/13 CRDT- 2017/05/17 06:00 PHST- 2017/03/16 00:00 [received] PHST- 2017/05/01 00:00 [revised] PHST- 2017/05/10 00:00 [accepted] PHST- 2017/05/17 06:00 [pubmed] PHST- 2018/02/23 06:00 [medline] PHST- 2017/05/17 06:00 [entrez] PHST- 2017/05/13 00:00 [pmc-release] AID - S0168-1702(17)30233-2 [pii] AID - 10.1016/j.virusres.2017.05.006 [doi] PST - ppublish SO - Virus Res. 2017 Jun 2;237:7-13. doi: 10.1016/j.virusres.2017.05.006. Epub 2017 May 13. PMID- 29511173 OWN - NLM STAT- MEDLINE DCOM- 20180820 LR - 20181114 IS - 2222-1751 (Electronic) IS - 2222-1751 (Linking) VI - 7 IP - 1 DP - 2018 Mar 7 TI - Rapid detection of MERS coronavirus-like viruses in bats: pote1ntial for tracking MERS coronavirus transmission and animal origin. PG - 18 LID - 10.1038/s41426-017-0016-7 [doi] LID - 18 AB - Recently, we developed a monoclonal antibody-based rapid nucleocapsid protein detection assay for diagnosis of MERS coronavirus (MERS-CoV) in humans and dromedary camels. In this study, we examined the usefulness of this assay to detect other lineage C betacoronaviruses closely related to MERS-CoV in bats. The rapid MERS-CoV nucleocapsid protein detection assay was tested positive in 24 (88.9%) of 27 Tylonycteris bat CoV HKU4 (Ty-BatCoV-HKU4) RNA-positive alimentary samples of Tylonycteris pachypus and 4 (19.0%) of 21 Pipistrellus bat CoV HKU5 (Pi-BatCoV-HKU5) RNA-positive alimentary samples of Pipistrellus abramus. There was significantly more Ty-BatCoV-HKU4 RNA-positive alimentary samples than Pi-BatCoV-HKU5 RNA-positive alimentary samples that were tested positive by the rapid MERS-CoV nucleocapsid protein detection assay (P < 0.001 by Chi-square test). The rapid assay was tested negative in all 51 alimentary samples RNA-positive for alphacoronaviruses (Rhinolophus bat CoV HKU2, Myotis bat CoV HKU6, Miniopterus bat CoV HKU8 and Hipposideros batCoV HKU10) and 32 alimentary samples positive for lineage B (SARS-related Rhinolophus bat CoV HKU3) and lineage D (Rousettus bat CoV HKU9) betacoronaviruses. No significant difference was observed between the viral loads of Ty-BatCoV-HKU4/Pi-BatCoV-HKU5 RNA-positive alimentary samples that were tested positive and negative by the rapid test (Mann-Witney U test). The rapid MERS-CoV nucleocapsid protein detection assay is able to rapidly detect lineage C betacoronaviruses in bats. It detected significantly more Ty-BatCoV-HKU4 than Pi-BatCoV-HKU5 because MERS-CoV is more closely related to Ty-BatCoV-HKU4 than Pi-BatCoV-HKU5. This assay will facilitate rapid on-site mass screening of animal samples for ancestors of MERS-CoV and tracking transmission in the related bat species. FAU - Woo, Patrick C Y AU - Woo PCY AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk. AD - Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk. AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk. FAU - Lau, Susanna K P AU - Lau SKP AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China. skplau@hku.hk. AD - Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China. skplau@hku.hk. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, China. skplau@hku.hk. AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. skplau@hku.hk. FAU - Chen, Yixin AU - Chen Y AD - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, 361102, Fujian, China. FAU - Wong, Emily Y M AU - Wong EYM AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Chan, Kwok-Hung AU - Chan KH AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Chen, Honglin AU - Chen H AUID- ORCID: 0000-0001-5108-8338 AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China. AD - Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, China. AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Zhang, Libiao AU - Zhang L AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China. FAU - Xia, Ningshao AU - Xia N AD - State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, 361102, Fujian, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China. AD - Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, China. AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. LA - eng PT - Journal Article DEP - 20180307 PL - United States TA - Emerg Microbes Infect JT - Emerging microbes & infections JID - 101594885 RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Chiroptera/classification/*virology MH - Coronavirus Infections/diagnosis/transmission/*veterinary/virology MH - Middle East Respiratory Syndrome Coronavirus/classification/genetics/*isolation & purification/metabolism MH - Phylogeny MH - Spike Glycoprotein, Coronavirus/genetics/metabolism PMC - PMC5841240 COIS- The authors declare no conflict of interest. EDAT- 2018/03/08 06:00 MHDA- 2018/08/21 06:00 PMCR- 2018/03/07 CRDT- 2018/03/08 06:00 PHST- 2017/07/14 00:00 [received] PHST- 2017/12/14 00:00 [accepted] PHST- 2017/11/28 00:00 [revised] PHST- 2018/03/08 06:00 [entrez] PHST- 2018/03/08 06:00 [pubmed] PHST- 2018/08/21 06:00 [medline] PHST- 2018/03/07 00:00 [pmc-release] AID - 10.1038/s41426-017-0016-7 [pii] AID - 16 [pii] AID - 10.1038/s41426-017-0016-7 [doi] PST - epublish SO - Emerg Microbes Infect. 2018 Mar 7;7(1):18. doi: 10.1038/s41426-017-0016-7. PMID- 26899616 OWN - NLM STAT- MEDLINE DCOM- 20170203 LR - 20240211 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 6 DP - 2016 Feb 22 TI - Replication and shedding of MERS-CoV in Jamaican fruit bats (Artibeus jamaicensis). PG - 21878 LID - 10.1038/srep21878 [doi] LID - 21878 AB - The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) highlights the zoonotic potential of Betacoronaviruses. Investigations into the origin of MERS-CoV have focused on two potential reservoirs: bats and camels. Here, we investigated the role of bats as a potential reservoir for MERS-CoV. In vitro, the MERS-CoV spike glycoprotein interacted with Jamaican fruit bat (Artibeus jamaicensis) dipeptidyl peptidase 4 (DPP4) receptor and MERS-CoV replicated efficiently in Jamaican fruit bat cells, suggesting there is no restriction at the receptor or cellular level for MERS-CoV. To shed light on the intrinsic host-virus relationship, we inoculated 10 Jamaican fruit bats with MERS-CoV. Although all bats showed evidence of infection, none of the bats showed clinical signs of disease. Virus shedding was detected in the respiratory and intestinal tract for up to 9 days. MERS-CoV replicated transiently in the respiratory and, to a lesser extent, the intestinal tracts and internal organs; with limited histopathological changes observed only in the lungs. Analysis of the innate gene expression in the lungs showed a moderate, transient induction of expression. Our results indicate that MERS-CoV maintains the ability to replicate in bats without clinical signs of disease, supporting the general hypothesis of bats as ancestral reservoirs for MERS-CoV. FAU - Munster, Vincent J AU - Munster VJ AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. FAU - Adney, Danielle R AU - Adney DR AD - Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA. FAU - van Doremalen, Neeltje AU - van Doremalen N AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. FAU - Brown, Vienna R AU - Brown VR AD - Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA. FAU - Miazgowicz, Kerri L AU - Miazgowicz KL AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. FAU - Milne-Price, Shauna AU - Milne-Price S AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. FAU - Bushmaker, Trenton AU - Bushmaker T AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. FAU - Rosenke, Rebecca AU - Rosenke R AD - Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. FAU - Scott, Dana AU - Scott D AD - Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. FAU - Hawkinson, Ann AU - Hawkinson A AD - Department of Biology, University of Northern Colorado, Greeley, Colorado, USA. FAU - de Wit, Emmie AU - de Wit E AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. FAU - Schountz, Tony AU - Schountz T AD - Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA. FAU - Bowen, Richard A AU - Bowen RA AD - Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA. LA - eng GR - R15 AI089419/AI/NIAID NIH HHS/United States GR - R15-AI089419/AI/NIAID NIH HHS/United States GR - ImNIH/Intramural NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, N.I.H., Intramural PT - Research Support, Non-U.S. Gov't DEP - 20160222 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Antibodies, Viral) RN - 0 (Receptors, Virus) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Animals MH - Antibodies, Viral/blood MH - Chiroptera/virology MH - Chlorocebus aethiops MH - Coronavirus Infections/blood/immunology/*veterinary/virology MH - Cricetinae MH - Dipeptidyl Peptidase 4/metabolism MH - Immunity, Innate MH - Lung/pathology/virology MH - Middle East Respiratory Syndrome Coronavirus/*physiology MH - Receptors, Virus/metabolism MH - Vero Cells MH - Viral Load MH - *Virus Replication MH - *Virus Shedding PMC - PMC4761889 EDAT- 2016/02/24 06:00 MHDA- 2017/02/06 06:00 PMCR- 2016/02/22 CRDT- 2016/02/23 06:00 PHST- 2015/08/28 00:00 [received] PHST- 2016/02/03 00:00 [accepted] PHST- 2016/02/23 06:00 [entrez] PHST- 2016/02/24 06:00 [pubmed] PHST- 2017/02/06 06:00 [medline] PHST- 2016/02/22 00:00 [pmc-release] AID - srep21878 [pii] AID - 10.1038/srep21878 [doi] PST - epublish SO - Sci Rep. 2016 Feb 22;6:21878. doi: 10.1038/srep21878. PMID- 27479465 OWN - NLM STAT- MEDLINE DCOM- 20170504 LR - 20210119 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 497 DP - 2016 Oct TI - Tubulins interact with porcine and human S proteins of the genus Alphacoronavirus and support successful assembly and release of infectious viral particles. PG - 185-197 LID - S0042-6822(16)30185-4 [pii] LID - 10.1016/j.virol.2016.07.022 [doi] AB - Coronavirus spike proteins mediate host-cell-attachment and virus entry. Virus replication takes place within the host cell cytosol, whereas assembly and budding occur at the endoplasmic reticulum-Golgi intermediate compartment. In this study we demonstrated that the last 39 amino acid stretches of Alphacoronavirus spike cytoplasmic domains of the human coronavirus 229E, NL63, and the porcine transmissible gastroenteritis virus TGEV interact with tubulin alpha and beta chains. In addition, a partial co-localization of TGEV spike proteins with authentic host cell β-tubulin was observed. Furthermore, drug-induced microtubule depolymerization led to changes in spike protein distribution, a reduction in the release of infectious virus particles and less amount of spike protein incorporated into virions. These data demonstrate that interaction of Alphacoronavirus spike proteins with tubulin supports S protein transport and incorporation into virus particles. CI - Copyright © 2016 Elsevier Inc. All rights reserved. FAU - Rüdiger, Anna-Theresa AU - Rüdiger AT AD - Institute of Virology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany. FAU - Mayrhofer, Peter AU - Mayrhofer P AD - Virology Department, Max-von-Pettenkofer Institute, Ludwig-Maximilians University Munich, Pettenkoferstraße 9a, 80336 Munich, Germany. FAU - Ma-Lauer, Yue AU - Ma-Lauer Y AD - Virology Department, Max-von-Pettenkofer Institute, Ludwig-Maximilians University Munich, Pettenkoferstraße 9a, 80336 Munich, Germany. FAU - Pohlentz, Gottfried AU - Pohlentz G AD - Institute for Hygiene, University of Münster, Robert-Koch-Straße 41, 48149 Münster, Germany. FAU - Müthing, Johannes AU - Müthing J AD - Institute for Hygiene, University of Münster, Robert-Koch-Straße 41, 48149 Münster, Germany. FAU - von Brunn, Albrecht AU - von Brunn A AD - Virology Department, Max-von-Pettenkofer Institute, Ludwig-Maximilians University Munich, Pettenkoferstraße 9a, 80336 Munich, Germany; German Centers for Infection Research (DZIF), Ludwig-Maximilians-University Munich, Germany. Electronic address: vonbrunn@mvp.uni-muenchen.de. FAU - Schwegmann-Weßels, Christel AU - Schwegmann-Weßels C AD - Institute of Virology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany. Electronic address: christel.schwegmann@tiho-hannover.de. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160730 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Tubulin) RN - SH1WY3R615 (Nocodazole) SB - IM MH - Animals MH - Cell Line MH - Coronaviridae/drug effects/*physiology MH - Coronaviridae Infections/*metabolism/*virology MH - Gastroenteritis, Transmissible, of Swine/metabolism/virology MH - Humans MH - Intracellular Space/metabolism/virology MH - Nocodazole/pharmacology MH - Protein Binding MH - Protein Interaction Domains and Motifs MH - Protein Transport MH - Spike Glycoprotein, Coronavirus/chemistry/*metabolism MH - Swine MH - Tubulin/*metabolism MH - *Virus Assembly/drug effects MH - Virus Release MH - *Virus Replication/drug effects PMC - PMC7111311 OTO - NOTNLM OT - Assembly OT - Coronavirus OT - Incorporation OT - Interaction OT - Intracellular transport OT - Microtubule OT - Spike protein OT - TGEV OT - Viral infectivity EDAT- 2016/08/02 06:00 MHDA- 2017/05/05 06:00 PMCR- 2016/07/30 CRDT- 2016/08/02 06:00 PHST- 2016/04/25 00:00 [received] PHST- 2016/07/16 00:00 [revised] PHST- 2016/07/18 00:00 [accepted] PHST- 2016/08/02 06:00 [entrez] PHST- 2016/08/02 06:00 [pubmed] PHST- 2017/05/05 06:00 [medline] PHST- 2016/07/30 00:00 [pmc-release] AID - S0042-6822(16)30185-4 [pii] AID - 10.1016/j.virol.2016.07.022 [doi] PST - ppublish SO - Virology. 2016 Oct;497:185-197. doi: 10.1016/j.virol.2016.07.022. Epub 2016 Jul 30. PMID- 31176408 OWN - NLM STAT- MEDLINE DCOM- 20190626 LR - 20200407 IS - 1873-2542 (Electronic) IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 233 DP - 2019 Jun TI - Susceptibility of porcine IPI-2I intestinal epithelial cells to infection with swine enteric coronaviruses. PG - 21-27 LID - S0378-1135(19)30155-5 [pii] LID - 10.1016/j.vetmic.2019.04.014 [doi] AB - Swine enteric coronavirus (CoV) is an important group of pathogens causing diarrhea in piglets. At least four kinds of swine enteric CoVs have been identified, including transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and the emerging HKU2-like porcine enteric alphacoronavirus (PEAV). The small intestines, particularly the jejunum and ileum, are the most common targets of these four CoVs in vivo, and co-infections by these CoVs are frequently observed in clinically infected pigs. This study was conducted to investigate the susceptibility of the porcine ileum epithelial cell line, IPI-2I, to different swine enteric CoVs. We found that IPI-2I cells are highly susceptible to TGEV, PDCoV, and PEAV, as demonstrated by cytopathic effect and virus multiplication. However, only a small number of cells could be infected by PEDV, possibly due to the heterogeneity of IPI-2I cells. A homogeneous cell line, designated IPI-FX, obtained from IPI-2I cells by sub-cloning with limited serial dilutions, was found to be highly susceptible to PEDV. Furthermore, IPI-FX cells were also highly susceptible to TGEV, PDCoV, as well as PEAV. Thus, this sub-cloned IPI-FX cell line is an ideal cell model to study the mechanisms of infection, particularly co-infections of swine enteric CoVs. CI - Copyright © 2019 Elsevier B.V. All rights reserved. FAU - Wang, Xunlei AU - Wang X AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Fang, Liurong AU - Fang L AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. Electronic address: fanglr@mail.hzau.edu.cn. FAU - Liu, Shudan AU - Liu S AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Ke, Wenting AU - Ke W AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Wang, Dang AU - Wang D AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Peng, Guiqing AU - Peng G AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. FAU - Xiao, Shaobo AU - Xiao S AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. LA - eng PT - Journal Article DEP - 20190414 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 SB - IM MH - Animals MH - Cell Culture Techniques/*veterinary MH - Cell Line MH - Coronavirus/*pathogenicity MH - Diarrhea/virology MH - Epithelial Cells/*virology MH - Feces/virology MH - Intestine, Small/*cytology MH - Phylogeny MH - Porcine epidemic diarrhea virus/genetics/*pathogenicity MH - Swine MH - Swine Diseases/virology MH - Virus Replication PMC - PMC7117161 OTO - NOTNLM OT - Porcine ileum epithelial cell (IPI-2I) OT - Susceptibility OT - Swine enteric coronavirus EDAT- 2019/06/10 06:00 MHDA- 2019/06/27 06:00 PMCR- 2019/04/14 CRDT- 2019/06/10 06:00 PHST- 2019/01/31 00:00 [received] PHST- 2019/04/08 00:00 [revised] PHST- 2019/04/10 00:00 [accepted] PHST- 2019/06/10 06:00 [entrez] PHST- 2019/06/10 06:00 [pubmed] PHST- 2019/06/27 06:00 [medline] PHST- 2019/04/14 00:00 [pmc-release] AID - S0378-1135(19)30155-5 [pii] AID - 10.1016/j.vetmic.2019.04.014 [doi] PST - ppublish SO - Vet Microbiol. 2019 Jun;233:21-27. doi: 10.1016/j.vetmic.2019.04.014. Epub 2019 Apr 14. PMID- 25787280 OWN - NLM STAT- MEDLINE DCOM- 20150727 LR - 20201209 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 11 DP - 2015 Jun TI - Receptor usage and cell entry of porcine epidemic diarrhea coronavirus. PG - 6121-5 LID - 10.1128/JVI.00430-15 [doi] AB - Porcine epidemic diarrhea coronavirus (PEDV) has significantly damaged America's pork industry. Here we investigate the receptor usage and cell entry of PEDV. PEDV recognizes protein receptor aminopeptidase N from pig and human and sugar coreceptor N-acetylneuraminic acid. Moreover, PEDV infects cells from pig, human, monkey, and bat. These results support the idea of bats as an evolutionary origin for PEDV, implicate PEDV as a potential threat to other species, and suggest antiviral strategies to control its spread. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Liu, Chang AU - Liu C AD - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA. FAU - Tang, Jian AU - Tang J AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA. FAU - Ma, Yuanmei AU - Ma Y AD - Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA. FAU - Liang, Xueya AU - Liang X AD - Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA. FAU - Yang, Yang AU - Yang Y AD - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA. FAU - Peng, Guiqing AU - Peng G AD - State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Qi, Qianqian AU - Qi Q AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College and Institute of Medical Microbiology, Fudan University, Shanghai, China. FAU - Jiang, Shibo AU - Jiang S AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College and Institute of Medical Microbiology, Fudan University, Shanghai, China. FAU - Li, Jianrong AU - Li J AD - Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA. FAU - Du, Lanying AU - Du L AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA ldu@nybloodcenter.org lifang@umn.edu. FAU - Li, Fang AU - Li F AD - Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA ldu@nybloodcenter.org lifang@umn.edu. LA - eng GR - R01 AI089728/AI/NIAID NIH HHS/United States GR - R01AI089728/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20150318 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Receptors, Coronavirus) RN - 0 (Receptors, Virus) RN - EC 3.4.11.2 (CD13 Antigens) RN - GZP2782OP0 (N-Acetylneuraminic Acid) SB - IM MH - Animals MH - CD13 Antigens/*metabolism MH - Chiroptera MH - Haplorhini MH - Humans MH - N-Acetylneuraminic Acid/*metabolism MH - Porcine epidemic diarrhea virus/*physiology MH - Receptors, Coronavirus MH - Receptors, Virus/*metabolism MH - Swine MH - *Virus Attachment MH - *Virus Internalization PMC - PMC4442452 EDAT- 2015/03/20 06:00 MHDA- 2015/07/28 06:00 PMCR- 2015/12/01 CRDT- 2015/03/20 06:00 PHST- 2015/02/15 00:00 [received] PHST- 2015/03/10 00:00 [accepted] PHST- 2015/03/20 06:00 [entrez] PHST- 2015/03/20 06:00 [pubmed] PHST- 2015/07/28 06:00 [medline] PHST- 2015/12/01 00:00 [pmc-release] AID - JVI.00430-15 [pii] AID - 00430-15 [pii] AID - 10.1128/JVI.00430-15 [doi] PST - ppublish SO - J Virol. 2015 Jun;89(11):6121-5. doi: 10.1128/JVI.00430-15. Epub 2015 Mar 18. PMID- 30531999 OWN - NLM STAT- MEDLINE DCOM- 20190422 LR - 20221207 IS - 2222-1751 (Electronic) IS - 2222-1751 (Linking) VI - 7 IP - 1 DP - 2018 Dec 10 TI - Replication of MERS and SARS coronaviruses in bat cells offers insights to their ancestral origins. PG - 209 LID - 10.1038/s41426-018-0208-9 [doi] LID - 209 AB - Previous findings of Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses in bats, and the ability of Tylonycteris-BatCoV HKU4 spike protein to utilize MERS-CoV receptor, human dipeptidyl peptidase 4 hDPP4, suggest a bat ancestral origin of MERS-CoV. We developed 12 primary bat cell lines from seven bat species, including Tylonycteris pachypus, Pipistrellus abramus and Rhinolophus sinicus (hosts of Tylonycteris-BatCoV HKU4, Pipistrellus-BatCoV HKU5, and SARS-related-CoV respectively), and tested their susceptibilities to MERS-CoVs, SARS-CoV, and human coronavirus 229E (HCoV-229E). Five cell lines, including P. abramus and R. sinicus but not T. pachypus cells, were susceptible to human MERS-CoV EMC/2012. However, three tested camel MERS-CoV strains showed different infectivities, with only two strains capable of infecting three and one cell lines respectively. SARS-CoV can only replicate in R. sinicus cells, while HCoV-229E cannot replicate in any bat cells. Bat dipeptidyl peptidase 4 (DPP4) sequences were closely related to those of human and non-human primates but distinct from dromedary DPP4 sequence. Critical residues for binding to MERS-CoV spike protein were mostly conserved in bat DPP4. DPP4 was expressed in the five bat cells susceptible to MERS-CoV, with significantly higher mRNA expression levels than those in non-susceptible cells (P = 0.0174), supporting that DPP4 expression is critical for MERS-CoV infection in bats. However, overexpression of T. pachypus DPP4 failed to confer MERS-CoV susceptibility in T. pachypus cells, suggesting other cellular factors in determining viral replication. The broad cellular tropism of MERS-CoV should prompt further exploration of host diversity of related viruses to identify its ancestral origin. FAU - Lau, Susanna K P AU - Lau SKP AD - State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. skplau@hku.hk. AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. skplau@hku.hk. AD - Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. skplau@hku.hk. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. skplau@hku.hk. FAU - Fan, Rachel Y Y AU - Fan RYY AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Luk, Hayes K H AU - Luk HKH AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Zhu, Longchao AU - Zhu L AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Fung, Joshua AU - Fung J AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Li, Kenneth S M AU - Li KSM AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Wong, Emily Y M AU - Wong EYM AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Ahmed, Syed Shakeel AU - Ahmed SS AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Chan, Jasper F W AU - Chan JFW AD - State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Kok, Raven K H AU - Kok RKH AUID- ORCID: 0000-0003-3426-332X AD - State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Chan, Kwok-Hung AU - Chan KH AD - State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Wernery, Ulrich AU - Wernery U AD - Central Veterinary Research Laboratory, Dubai, United Arab Emirates. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. FAU - Woo, Patrick C Y AU - Woo PCY AD - State Key Laboratory of Emerging Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk. AD - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk. AD - Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. pcywoo@hku.hk. LA - eng PT - Journal Article DEP - 20181210 PL - United States TA - Emerg Microbes Infect JT - Emerging microbes & infections JID - 101594885 RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Animals MH - Camelus MH - Cell Line MH - Cells, Cultured MH - Chiroptera/*virology MH - Dipeptidyl Peptidase 4/genetics MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/genetics/*physiology MH - Phylogeny MH - Primates MH - Severe acute respiratory syndrome-related coronavirus/genetics/*physiology MH - Spike Glycoprotein, Coronavirus/metabolism MH - Viral Tropism MH - Virus Attachment MH - *Virus Replication PMC - PMC6286955 COIS- The authors declare that they have no conflict of interest. EDAT- 2018/12/12 06:00 MHDA- 2019/04/23 06:00 PMCR- 2018/12/10 CRDT- 2018/12/12 06:00 PHST- 2018/08/24 00:00 [received] PHST- 2018/11/11 00:00 [accepted] PHST- 2018/11/08 00:00 [revised] PHST- 2018/12/12 06:00 [entrez] PHST- 2018/12/12 06:00 [pubmed] PHST- 2019/04/23 06:00 [medline] PHST- 2018/12/10 00:00 [pmc-release] AID - 10.1038/s41426-018-0208-9 [pii] AID - 208 [pii] AID - 10.1038/s41426-018-0208-9 [doi] PST - epublish SO - Emerg Microbes Infect. 2018 Dec 10;7(1):209. doi: 10.1038/s41426-018-0208-9. PMID- 27696819 OWN - NLM STAT- MEDLINE DCOM- 20170502 LR - 20200318 IS - 1520-4995 (Electronic) IS - 0006-2960 (Print) IS - 0006-2960 (Linking) VI - 55 IP - 43 DP - 2016 Nov 1 TI - Putative Receptor Binding Domain of Bat-Derived Coronavirus HKU9 Spike Protein: Evolution of Betacoronavirus Receptor Binding Motifs. PG - 5977-5988 AB - The suggested bat origin for Middle East respiratory syndrome coronavirus (MERS-CoV) has revitalized the studies of other bat-derived coronaviruses with respect to interspecies transmission potential. Bat coronavirus (BatCoV) HKU9 is an important betacoronavirus (betaCoV) that is phylogenetically affiliated with the same genus as MERS-CoV. The bat surveillance data indicated that BatCoV HKU9 has been widely spreading and circulating in bats. This highlights the necessity of characterizing the virus for its potential to cross species barriers. The receptor binding domain (RBD) of the coronavirus spike (S) protein recognizes host receptors to mediate virus entry and is therefore a key factor determining the viral tropism and transmission capacity. In this study, the putative S RBD of BatCoV HKU9 (HKU9-RBD), which is homologous to other betaCoV RBDs that have been structurally and functionally defined, was characterized via a series of biophysical and crystallographic methods. By using surface plasmon resonance, we demonstrated that HKU9-RBD binds to neither SARS-CoV receptor ACE2 nor MERS-CoV receptor CD26. We further determined the atomic structure of HKU9-RBD, which as expected is composed of a core and an external subdomain. The core subdomain fold resembles those of other betaCoV RBDs, whereas the external subdomain is structurally unique with a single helix, explaining the inability of HKU9-RBD to react with either ACE2 or CD26. Via comparison of the available RBD structures, we further proposed a homologous intersubdomain binding mode in betaCoV RBDs that anchors the external subdomain to the core subdomain. The revealed RBD features would shed light on the evolution route of betaCoV. FAU - Huang, Canping AU - Huang C AD - National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC) , Beijing 102206, China. FAU - Qi, Jianxun AU - Qi J AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, China. FAU - Lu, Guangwen AU - Lu G AD - West China Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China. FAU - Wang, Qihui AU - Wang Q AD - CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, China. FAU - Yuan, Yuan AU - Yuan Y AD - School of Life Sciences, University of Science and Technology of China , Hefei, Anhui Province 230026, China. FAU - Wu, Ying AU - Wu Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, China. FAU - Zhang, Yanfang AU - Zhang Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, China. FAU - Yan, Jinghua AU - Yan J AD - CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, China. FAU - Gao, George F AU - Gao GF AD - National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC) , Beijing 102206, China. AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101, China. AD - School of Life Sciences, University of Science and Technology of China , Hefei, Anhui Province 230026, China. AD - Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China. AD - Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences , Beijing 100101, China. LA - eng PT - Journal Article DEP - 20161018 PL - United States TA - Biochemistry JT - Biochemistry JID - 0370623 RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Binding Sites MH - Chiroptera MH - Coronavirus/chemistry/classification MH - Crystallography, X-Ray MH - Phylogeny MH - Protein Conformation MH - Spike Glycoprotein, Coronavirus/chemistry/*metabolism MH - Surface Plasmon Resonance PMC - PMC7075523 COIS- The authors declare no competing financial interest. EDAT- 2016/11/02 06:00 MHDA- 2017/05/04 06:00 PMCR- 2020/03/16 CRDT- 2016/10/05 06:00 PHST- 2016/11/02 06:00 [pubmed] PHST- 2017/05/04 06:00 [medline] PHST- 2016/10/05 06:00 [entrez] PHST- 2020/03/16 00:00 [pmc-release] AID - 10.1021/acs.biochem.6b00790 [doi] PST - ppublish SO - Biochemistry. 2016 Nov 1;55(43):5977-5988. doi: 10.1021/acs.biochem.6b00790. Epub 2016 Oct 18. PMID- 26330152 OWN - NLM STAT- MEDLINE DCOM- 20160629 LR - 20221207 IS - 1961-9049 (Electronic) IS - 0037-9085 (Print) IS - 0037-9085 (Linking) VI - 108 IP - 4 DP - 2015 Oct TI - [Bats and Viruses: complex relationships]. PG - 272-89 LID - 10.1007/s13149-015-0448-z [doi] AB - With more than 1 200 species, bats and flying foxes (Order Chiroptera) constitute the most important and diverse order of Mammals after Rodents. Many species of bats are insectivorous while others are frugivorous and few of them are hematophagous. Some of these animals fly during the night, others are crepuscular or diurnal. Some fly long distances during seasonal migrations. Many species are colonial cave-dwelling, living in a rather small home range while others are relatively solitary. However, in spite of the importance of bats for terrestrial biotic communities and ecosystem ecology, the diversity in their biology and lifestyles remain poorly known and underappreciated. More than sixty viruses have been detected or isolated in bats; these animals are therefore involved in the natural cycles of many of them. This is the case, for instance, of rabies virus and other Lyssavirus (Family Rhabdoviridae), Nipah and Hendra viruses (Paramyxoviridae), Ebola and Marburg viruses (Filoviridae), SARS-CoV and MERS-CoV (Coronaviridae). For these zoonotic viruses, a number of bat species are considered as important reservoir hosts, efficient disseminators or even directly responsible of the transmission. Some of these bat-borne viruses cause highly pathogenic diseases while others are of potential significance for humans and domestic or wild animals; so, bats are an important risk in human and animal public health. Moreover, some groups of viruses developed through different phylogenetic mechanisms of coevolution between viruses and bats. The fact that most of these viral infections are asymptomatic in bats has been observed since a long time but the mechanisms of the viral persistence are not clearly understood. The various bioecology of the different bat populations allows exchange of virus between migrating and non-migrating conspecific species. For a better understanding of the role of bats in the circulation of these viral zoonoses, epidemiologists must pay attention to some of their biologic properties which are not fully documented, like their extreme longevity, their diet, the population size and the particular densities observed in species with crowded roosting behavior, the population structure and migrations, the hibernation permitting overwintering of viruses, their particular innate and acquired immune response, probably related at least partially to their ability to fly, allowing persistent virus infections and preventing immunopathological consequences, etc. It is also necessary to get a better knowledge of the interactions between bats and ecologic changes induced by man and to attentively follow bat populations and their viruses through surveillance networks involving human and veterinary physicians, specialists of wild fauna, ecologists, etc. in order to understand the mechanisms of disease emergence, to try to foresee and, perhaps, to prevent viral emergences beforehand. Finally, a more fundamental research about immune mechanisms developed in viral infections is essential to reveal the reasons why Chiroptera are so efficient reservoir hosts. Clearly, a great deal of additional work is needed to document the roles of bats in the natural history of viruses. FAU - Rodhain, F AU - Rodhain F AD - Professeur honoraire à l'Institut Pasteur, 132, boulevard du Montparnasse, 75014, Paris, France. francoisrodhain@gmail.com. LA - fre PT - English Abstract PT - Journal Article PT - Review TT - Chauves-souris et virus: des relations complexes. DEP - 20150901 PL - France TA - Bull Soc Pathol Exot JT - Bulletin de la Societe de pathologie exotique (1990) JID - 9212564 SB - IM MH - Animals MH - Chiroptera/*virology MH - Disease Reservoirs/*virology MH - Ecology MH - Hemorrhagic Fever, Ebola/epidemiology/virology MH - Humans MH - Male MH - Middle East Respiratory Syndrome Coronavirus/isolation & purification/physiology MH - Public Health MH - Severe acute respiratory syndrome-related coronavirus/isolation & purification/physiology MH - Virus Diseases/epidemiology/transmission MH - Viruses/classification/immunology/*isolation & purification MH - Zoonoses/*epidemiology/transmission/virology PMC - PMC7097034 OAB - With more than 1 200 species, bats and flying foxes (Order Chiroptera) constitute the most important and diverse order of Mammals after Rodents. Many species of bats are insectivorous while others are frugivorous and few of them are hematophagous. Some of these animals fly during the night, others are crepuscular or diurnal. Some fly long distances during seasonal migrations. Many species are colonial cave-dwelling, living in a rather small home range while others are relatively solitary. However, in spite of the importance of bats for terrestrial biotic communities and ecosystem ecology, the diversity in their biology and lifestyles remain poorly known and underappreciated. More than sixty viruses have been detected or isolated in bats; these animals are therefore involved in the natural cycles of many of them. This is the case, for instance, of rabies virus and other Lyssavirus (Family Rhabdoviridae), Nipah and Hendra viruses (Paramyxoviridae), Ebola and Marburg viruses (Filoviridae), SARS-CoV and MERS-CoV (Coronaviridae). For these zoonotic viruses, a number of bat species are considered as important reservoir hosts, efficient disseminators or even directly responsible of the transmission. Some of these bat-borne viruses cause highly pathogenic diseases while others are of potential significance for humans and domestic or wild animals; so, bats are an important risk in human and animal public health. Moreover, some groups of viruses developed through different phylogenetic mechanisms of coevolution between viruses and bats. The fact that most of these viral infections are asymptomatic in bats has been observed since a long time but the mechanisms of the viral persistence are not clearly understood. The various bioecology of the different bat populations allows exchange of virus between migrating and non-migrating conspecific species. For a better understanding of the role of bats in the circulation of these viral zoonoses, epidemiologists must pay attention to some of their biologic properties which are not fully documented, like their extreme longevity, their diet, the population size and the particular densities observed in species with crowded roosting behavior, the population structure and migrations, the hibernation permitting overwintering of viruses, their particular innate and acquired immune response, probably related at least partially to their ability to fly, allowing persistent virus infections and preventing immunopathological consequences, etc. It is also necessary to get a better knowledge of the interactions between bats and ecologic changes induced by man and to attentively follow bat populations and their viruses through surveillance networks involving human and veterinary physicians, specialists of wild fauna, ecologists, etc. in order to understand the mechanisms of disease emergence, to try to foresee and, perhaps, to prevent viral emergences beforehand. Finally, a more fundamental research about immune mechanisms developed in viral infections is essential to reveal the reasons why Chiroptera are so efficient reservoir hosts. Clearly, a great deal of additional work is needed to document the roles of bats in the natural history of viruses. OABL- eng OTO - NOTNLM OT - Bats OT - Chiroptera OT - Emerging epidemics OT - Epidemiology OT - Immune evasion and Virus persistence OT - Man OT - Viral zoonoses OT - Virus EDAT- 2015/09/04 06:00 MHDA- 2016/06/30 06:00 PMCR- 2020/03/26 CRDT- 2015/09/03 06:00 PHST- 2014/01/20 00:00 [received] PHST- 2015/07/28 00:00 [accepted] PHST- 2015/09/03 06:00 [entrez] PHST- 2015/09/04 06:00 [pubmed] PHST- 2016/06/30 06:00 [medline] PHST- 2020/03/26 00:00 [pmc-release] AID - 10.1007/s13149-015-0448-z [pii] AID - 448 [pii] AID - 10.1007/s13149-015-0448-z [doi] PST - ppublish SO - Bull Soc Pathol Exot. 2015 Oct;108(4):272-89. doi: 10.1007/s13149-015-0448-z. Epub 2015 Sep 1. PMID- 24511826 OWN - NLM STAT- MEDLINE DCOM- 20150409 LR - 20140211 IS - 0005-9366 (Print) IS - 0005-9366 (Linking) VI - 126 IP - 11-12 DP - 2013 Nov-Dec TI - [Bats, viruses and humans: coronaviruses on the rise?]. PG - 509-13 AB - The outbreak of the SARS coronavirus in 2002/2003 and the recent disease cases with a new human coronavirus (originally designated EMC-CoV, recently renamed MERS-CoV) have put the focus onto the virus family Coronaviridae. Both viruses appeared to have managed to jump over the species barrier from a bat reservoir to the human population. Bats are considered to serve as a natural reservoir for coronaviruses infecting mammals. An important factor for crossing the species-barrier is the adaptation to a new receptor on cells of the new host species. During evolution coronaviruses have developed a large diversity of binding specificities demonstrating the high flexibility of the coronaviral spike protein, which is responsible for binding to target cells. FAU - Winter, Christine AU - Winter C AD - Institut für Virologie der Tierärztliche Hochschule Hannover. christine.winter@tiho-hannover.de FAU - Herrler, Georg AU - Herrler G AD - Institut für Virologie der Tierärztliche Hochschule Hannover. LA - ger PT - English Abstract PT - Journal Article TT - Von Fledermläusen, Viren und Menschen: Coronaviren auf dem Vormarsch? PL - Germany TA - Berl Munch Tierarztl Wochenschr JT - Berliner und Munchener tierarztliche Wochenschrift JID - 0003163 RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronaviridae/*physiology MH - Coronaviridae Infections/epidemiology/*transmission MH - Disease Reservoirs/*virology MH - Humans MH - Spike Glycoprotein, Coronavirus/*metabolism EDAT- 2014/02/12 06:00 MHDA- 2015/04/10 06:00 CRDT- 2014/02/12 06:00 PHST- 2014/02/12 06:00 [entrez] PHST- 2014/02/12 06:00 [pubmed] PHST- 2015/04/10 06:00 [medline] PST - ppublish SO - Berl Munch Tierarztl Wochenschr. 2013 Nov-Dec;126(11-12):509-13. PMID- 29618640 OWN - NLM STAT- MEDLINE DCOM- 20180619 LR - 20201209 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 12 DP - 2018 Jun 15 TI - Porcine Deltacoronavirus Engages the Transmissible Gastroenteritis Virus Functional Receptor Porcine Aminopeptidase N for Infectious Cellular Entry. LID - 10.1128/JVI.00318-18 [doi] LID - e00318-18 AB - Identification of cellular receptors used by coronavirus (CoV) entry into the host cells is critical to an understanding of pathogenesis and to development of intervention strategies. The fourth CoV genus, Deltacoronavirus, evolutionarily related to the Gammacoronavirus, has just been defined recently. In the current study, we demonstrate that porcine aminopeptidase N (pAPN) acts as a cross-genus CoV functional receptor for both enteropathogenic porcine deltacoronovirus (PDCoV) and alphacoronovirus (AlphaCoV) (transmissible gastroenteritis virus [TGEV]) based upon three lines of evidence. First, the soluble S1 protein of PDCoV bound to the surface of target porcine cell lines known to express pAPN as efficiently as TGEV-S1, which could be blocked by soluble pAPN pretreatment. Second, both PDCoV-S1 and TGEV-S1 physically recognized and interacted with pAPN by coimmunoprecipitation in pAPN cDNA-transfected cells and by dot blot hybridization assay. Finally, exogenous expression of pAPN in refractory cells conferred susceptibility to PDCoV-S1 binding and to PDCoV entry and productive infection. PDCoV-S1 appeared to have a lower pAPN-binding affinity and likely consequent lower infection efficiency in pAPN-expressing refractory cells than TGEV-S1, suggesting that there may be differences between these two viruses in the virus-binding regions in pAPN. This study paves the way for dissecting the molecular mechanisms of PDCoV-host interactions and pathogenesis as well as facilitates future vaccine development and intervention strategies against PDCoV infection.IMPORTANCE The emergence of new human and animal coronaviruses is believed to have occurred through interspecies transmission that is mainly mediated by a species-specific receptor of the host. Among the four genera of the Coronavirinae, a couple of functional receptors for the representative members in the genera Alphacoronavirus and Betacoronavirus have been identified, whereas receptors for Gammacoronavirus and Deltacoronavirus, which are believed to originate from birds, are still unknown. Porcine coronaviruses, including the newly discovered porcine deltacoronavirus (PDCoV) associated with diarrhea in newborn piglets, have posed a serious threat to the pork industry in Asia and North America. Here, we report that PDCoV employs the alphacoronavirus TGEV functional receptor porcine aminopeptidase N (pAPN) for cellular entry, demonstrating the usage of pAPN as a cross-genus CoV functional receptor. The identification of the PDCoV receptor provides another example of the expanded host range of CoV and paves the way for further investigation of PDCoV-host interaction and pathogenesis. CI - Copyright © 2018 American Society for Microbiology. FAU - Wang, Bin AU - Wang B AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Liu, Yan AU - Liu Y AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Ji, Chun-Miao AU - Ji CM AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Yang, Yong-Le AU - Yang YL AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Liang, Qi-Zhang AU - Liang QZ AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Zhao, Pengwei AU - Zhao P AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Xu, Ling-Dong AU - Xu LD AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Lei, Xi-Mei AU - Lei XM AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Luo, Wen-Ting AU - Luo WT AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Qin, Pan AU - Qin P AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Zhou, Jiyong AU - Zhou J AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Huang, Yao-Wei AU - Huang YW AUID- ORCID: 0000-0001-9755-8411 AD - Institute of Preventive Veterinary Medicine and Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China yhuang@zju.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180529 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Receptors, Coronavirus) RN - 0 (Receptors, Virus) RN - EC 3.4.11.2 (CD13 Antigens) SB - IM MH - Animals MH - CD13 Antigens/*metabolism MH - Cell Line MH - Chlorocebus aethiops MH - Coronavirus/*metabolism MH - Coronavirus Infections/pathology/virology MH - Cricetinae MH - Host Specificity/genetics MH - Receptors, Coronavirus MH - Receptors, Virus/genetics/*metabolism MH - Swine MH - Swine Diseases/pathology/virology MH - Transmissible gastroenteritis virus/*metabolism MH - Vero Cells MH - *Virus Attachment MH - Virus Internalization PMC - PMC5974500 OTO - NOTNLM OT - APN OT - PDCoV OT - aminopeptidase N OT - cellular receptor OT - coronavirus OT - entry OT - porcine deltacoronavirus OT - virus entry EDAT- 2018/04/06 06:00 MHDA- 2018/06/21 06:00 PMCR- 2018/11/29 CRDT- 2018/04/06 06:00 PHST- 2018/02/26 00:00 [received] PHST- 2018/03/27 00:00 [accepted] PHST- 2018/04/06 06:00 [pubmed] PHST- 2018/06/21 06:00 [medline] PHST- 2018/04/06 06:00 [entrez] PHST- 2018/11/29 00:00 [pmc-release] AID - JVI.00318-18 [pii] AID - 00318-18 [pii] AID - 10.1128/JVI.00318-18 [doi] PST - epublish SO - J Virol. 2018 May 29;92(12):e00318-18. doi: 10.1128/JVI.00318-18. Print 2018 Jun 15. PMID- 28516065 OWN - NLM STAT- MEDLINE DCOM- 20171227 LR - 20181113 IS - 2235-2988 (Electronic) IS - 2235-2988 (Linking) VI - 7 DP - 2017 TI - miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1. PG - 155 LID - 10.3389/fcimb.2017.00155 [doi] LID - 155 AB - Porcine hemagglutinating encephalomyelitis virus (PHEV) invades the central nervous system (CNS) and causes neurodegenerative disease in suckling piglets, but the understanding of its neuropathogenicity for neurological dysfunction remains limited. Here, we report that miR-142-5p is localized to neurons and negatively regulates neuronal morphogenesis in porcine hemagglutinating encephalomyelitis (PHE). This phenotype was mediated by miR-142-5p inhibition of an mRNA encoding unc-51-like-kinase1 (Ulk1), which controls axon outgrowth and dendrite formation. Modulating miR-142-5p activity by microRNA mimics or inhibitors induced neurodegeneration, including stunted axon elongation, unstable dendritic spine formation, and irregular swelling and disconnection in neurites. Relieving Ulk1 mRNA repression in primary cortical neurons by miR-142-5p antagomirs or replication-deficient adenoviruses encoding Ulk1 (Ad5-Ulk1), which improved rescue of nerve injury, restricted viral replication, and increased survival rate in mice underlying PHEV infection. In contrast, disrupting Ulk1 in RNAi-expressing neurons mostly led to significantly shortened axon elongation and/or an abnormally large number of branched dendrites. Taken together, we demonstrated that the abnormal neuronal morphogenesis underlying PHEV infection was mainly caused by functional mRNA repression of the miR-142-5p target Ulk1. Our data revealed that PHEV adapted to use spatiotemporal control of host microRNAs to invade CNS, and provided new insights into the virus-associated neurological dysfunction microenvironment. FAU - Li, Zi AU - Li Z AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Lan, Yungang AU - Lan Y AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Zhao, Kui AU - Zhao K AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Lv, Xiaoling AU - Lv X AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Ding, Ning AU - Ding N AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Lu, Huijun AU - Lu H AD - Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin UniversityChangchun, China. FAU - Zhang, Jing AU - Zhang J AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Yue, Huiqing AU - Yue H AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Shi, Junchao AU - Shi J AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Song, Deguang AU - Song D AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - Gao, Feng AU - Gao F AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. FAU - He, Wenqi AU - He W AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin UniversityChangchun, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170503 PL - Switzerland TA - Front Cell Infect Microbiol JT - Frontiers in cellular and infection microbiology JID - 101585359 RN - 0 (MicroRNAs) RN - 0 (Mirn142 microRNA, mouse) RN - EC 2.7.11.1 (Autophagy-Related Protein-1 Homolog) RN - EC 2.7.11.1 (Ulk1 protein, mouse) SB - IM MH - Animals MH - Autophagy-Related Protein-1 Homolog/*drug effects/genetics/*metabolism/physiology MH - Axons/drug effects/pathology/virology MH - Betacoronavirus 1/*metabolism MH - Cell Proliferation/drug effects MH - Cerebral Cortex/drug effects/pathology/virology MH - Coronavirus Infections/virology MH - Dendrites/drug effects/pathology/virology MH - Disease Models, Animal MH - Gene Expression Regulation MH - Host-Pathogen Interactions MH - Male MH - Mice MH - Mice, Inbred BALB C MH - MicroRNAs/*antagonists & inhibitors/genetics/pharmacology MH - Morphogenesis/*drug effects MH - Neurons/*drug effects/pathology/*virology MH - Rats MH - Rats, Sprague-Dawley MH - Virus Replication/drug effects PMC - PMC5413507 OTO - NOTNLM OT - Porcine hemagglutinating encephalomyelitis virus OT - Ulk1 OT - miR-142-5p OT - neuronal morphogenesis OT - neurotropic virus EDAT- 2017/05/19 06:00 MHDA- 2017/12/28 06:00 PMCR- 2017/01/01 CRDT- 2017/05/19 06:00 PHST- 2017/01/21 00:00 [received] PHST- 2017/04/12 00:00 [accepted] PHST- 2017/05/19 06:00 [entrez] PHST- 2017/05/19 06:00 [pubmed] PHST- 2017/12/28 06:00 [medline] PHST- 2017/01/01 00:00 [pmc-release] AID - 10.3389/fcimb.2017.00155 [doi] PST - epublish SO - Front Cell Infect Microbiol. 2017 May 3;7:155. doi: 10.3389/fcimb.2017.00155. eCollection 2017. PMID- 26378164 OWN - NLM STAT- MEDLINE DCOM- 20160301 LR - 20201209 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 23 DP - 2015 Dec TI - Evidence for an Ancestral Association of Human Coronavirus 229E with Bats. PG - 11858-70 LID - 10.1128/JVI.01755-15 [doi] AB - We previously showed that close relatives of human coronavirus 229E (HCoV-229E) exist in African bats. The small sample and limited genomic characterizations have prevented further analyses so far. Here, we tested 2,087 fecal specimens from 11 bat species sampled in Ghana for HCoV-229E-related viruses by reverse transcription-PCR (RT-PCR). Only hipposiderid bats tested positive. To compare the genetic diversity of bat viruses and HCoV-229E, we tested historical isolates and diagnostic specimens sampled globally over 10 years. Bat viruses were 5- and 6-fold more diversified than HCoV-229E in the RNA-dependent RNA polymerase (RdRp) and spike genes. In phylogenetic analyses, HCoV-229E strains were monophyletic and not intermixed with animal viruses. Bat viruses formed three large clades in close and more distant sister relationships. A recently described 229E-related alpaca virus occupied an intermediate phylogenetic position between bat and human viruses. According to taxonomic criteria, human, alpaca, and bat viruses form a single CoV species showing evidence for multiple recombination events. HCoV-229E and the alpaca virus showed a major deletion in the spike S1 region compared to all bat viruses. Analyses of four full genomes from 229E-related bat CoVs revealed an eighth open reading frame (ORF8) located at the genomic 3' end. ORF8 also existed in the 229E-related alpaca virus. Reanalysis of HCoV-229E sequences showed a conserved transcription regulatory sequence preceding remnants of this ORF, suggesting its loss after acquisition of a 229E-related CoV by humans. These data suggested an evolutionary origin of 229E-related CoVs in hipposiderid bats, hypothetically with camelids as intermediate hosts preceding the establishment of HCoV-229E. IMPORTANCE: The ancestral origins of major human coronaviruses (HCoVs) likely involve bat hosts. Here, we provide conclusive genetic evidence for an evolutionary origin of the common cold virus HCoV-229E in hipposiderid bats by analyzing a large sample of African bats and characterizing several bat viruses on a full-genome level. Our evolutionary analyses show that animal and human viruses are genetically closely related, can exchange genetic material, and form a single viral species. We show that the putative host switches leading to the formation of HCoV-229E were accompanied by major genomic changes, including deletions in the viral spike glycoprotein gene and loss of an open reading frame. We reanalyze a previously described genetically related alpaca virus and discuss the role of camelids as potential intermediate hosts between bat and human viruses. The evolutionary history of HCoV-229E likely shares important characteristics with that of the recently emerged highly pathogenic Middle East respiratory syndrome (MERS) coronavirus. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Corman, Victor Max AU - Corman VM AUID- ORCID: 0000-0002-3605-0136 AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany German Centre for Infection Research, (DZIF), Partner Site Bonn-Cologne, Bonn, Germany. FAU - Baldwin, Heather J AU - Baldwin HJ AD - Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Ulm, Germany Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia. FAU - Tateno, Adriana Fumie AU - Tateno AF AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany Laboratório de Virologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil. FAU - Zerbinati, Rodrigo Melim AU - Zerbinati RM AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany Laboratório de Virologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil. FAU - Annan, Augustina AU - Annan A AD - Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana. FAU - Owusu, Michael AU - Owusu M AD - Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana. FAU - Nkrumah, Evans Ewald AU - Nkrumah EE AD - Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana. FAU - Maganga, Gael Darren AU - Maganga GD AD - Centre International de Recherches Médicales de Franceville, Franceville, Gabon. FAU - Oppong, Samuel AU - Oppong S AD - Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. FAU - Adu-Sarkodie, Yaw AU - Adu-Sarkodie Y AD - Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. FAU - Vallo, Peter AU - Vallo P AD - Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Ulm, Germany Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic. FAU - da Silva Filho, Luiz Vicente Ribeiro Ferreira AU - da Silva Filho LV AD - Laboratório de Virologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil Instituto da Criança, Hospital das Clínicas da FMUSP, São Paulo, Brazil. FAU - Leroy, Eric M AU - Leroy EM AD - Centre International de Recherches Médicales de Franceville, Franceville, Gabon Institut de Recherche pour le Développement, UMR 224 (MIVEGEC), IRD/CNRS/UM1, Montpellier, France. FAU - Thiel, Volker AU - Thiel V AD - Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty Bern, University of Bern, Bern, Switzerland Federal Department of Home Affairs, Institute of Virology and Immunology, Bern and Mittelhäusern, Switzerland. FAU - van der Hoek, Lia AU - van der Hoek L AD - Department of Medical Microbiology, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands. FAU - Poon, Leo L M AU - Poon LL AD - School of Public Health, The University of Hong Kong, Hong Kong, Special Administrative Region, People's Republic of China. FAU - Tschapka, Marco AU - Tschapka M AD - Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Ulm, Germany Smithsonian Tropical Research Institute, Balboa, Panama. FAU - Drosten, Christian AU - Drosten C AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany German Centre for Infection Research, (DZIF), Partner Site Bonn-Cologne, Bonn, Germany drosten@virology-bonn.de drexler@virology-bonn.de. FAU - Drexler, Jan Felix AU - Drexler JF AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany German Centre for Infection Research, (DZIF), Partner Site Bonn-Cologne, Bonn, Germany drosten@virology-bonn.de drexler@virology-bonn.de. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150916 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (DNA Primers) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Animals MH - Base Sequence MH - Bayes Theorem MH - *Biological Evolution MH - Camelids, New World/virology MH - Chiroptera/*virology MH - Coronavirus 229E, Human/*genetics MH - DNA Primers/genetics MH - Feces/virology MH - *Genetic Variation MH - Ghana MH - Humans MH - Models, Genetic MH - Molecular Sequence Data MH - *Phylogeny MH - RNA-Dependent RNA Polymerase/genetics MH - Reverse Transcriptase Polymerase Chain Reaction MH - Sequence Analysis, DNA MH - Spike Glycoprotein, Coronavirus/genetics PMC - PMC4645311 EDAT- 2015/09/18 06:00 MHDA- 2016/03/02 06:00 PMCR- 2016/06/01 CRDT- 2015/09/18 06:00 PHST- 2015/07/09 00:00 [received] PHST- 2015/09/07 00:00 [accepted] PHST- 2015/09/18 06:00 [entrez] PHST- 2015/09/18 06:00 [pubmed] PHST- 2016/03/02 06:00 [medline] PHST- 2016/06/01 00:00 [pmc-release] AID - JVI.01755-15 [pii] AID - 01755-15 [pii] AID - 10.1128/JVI.01755-15 [doi] PST - ppublish SO - J Virol. 2015 Dec;89(23):11858-70. doi: 10.1128/JVI.01755-15. Epub 2015 Sep 16. PMID- 28738245 OWN - NLM STAT- MEDLINE DCOM- 20170829 LR - 20221207 IS - 1096-0341 (Electronic) IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 510 DP - 2017 Oct TI - Two-amino acids change in the nsp4 of SARS coronavirus abolishes viral replication. PG - 165-174 LID - S0042-6822(17)30238-6 [pii] LID - 10.1016/j.virol.2017.07.019 [doi] AB - Infection with coronavirus rearranges the host cell membrane to assemble a replication/transcription complex in which replication of the viral genome and transcription of viral mRNA occur. Although coexistence of nsp3 and nsp4 is known to cause membrane rearrangement, the mechanisms underlying the interaction of these two proteins remain unclear. We demonstrated that binding of nsp4 with nsp3 is essential for membrane rearrangement and identified amino acid residues in nsp4 responsible for the interaction with nsp3. In addition, we revealed that the nsp3-nsp4 interaction is not sufficient to induce membrane rearrangement, suggesting the participation of other factors such as host proteins. Finally, we showed that loss of the nsp3-nsp4 interaction eliminated viral replication by using an infectious cDNA clone and replicon system of SARS-CoV. These findings provide clues to the mechanism of the replication/transcription complex assembly of SARS-CoV and could reveal an antiviral target for the treatment of betacoronavirus infection. CI - Copyright © 2017 Elsevier Inc. All rights reserved. FAU - Sakai, Yusuke AU - Sakai Y AD - Laboratory of Clinical Research on Infectious Diseases, Osaka, Japan; Laboratory of Veterinary Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan. FAU - Kawachi, Kengo AU - Kawachi K AD - Laboratory of Clinical Research on Infectious Diseases, Osaka, Japan; Department of Molecular Virology, Osaka, Japan. FAU - Terada, Yutaka AU - Terada Y AD - Laboratory of Clinical Research on Infectious Diseases, Osaka, Japan. FAU - Omori, Hiroko AU - Omori H AD - Core Instrumentation Facility, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan. FAU - Matsuura, Yoshiharu AU - Matsuura Y AD - Department of Molecular Virology, Osaka, Japan. FAU - Kamitani, Wataru AU - Kamitani W AD - Laboratory of Clinical Research on Infectious Diseases, Osaka, Japan; Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Tsukuba, Ibaraki 305-0843, Japan. Electronic address: wakamita@biken.osaka-u.ac.jp. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170721 PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Viral Nonstructural Proteins) SB - IM MH - *Amino Acid Substitution MH - DNA Mutational Analysis MH - Protein Interaction Mapping MH - Severe acute respiratory syndrome-related coronavirus/genetics/*physiology MH - Viral Nonstructural Proteins/*genetics MH - *Virus Replication PMC - PMC7111695 OTO - NOTNLM OT - Coronavirus OT - Nsp4 OT - Severe acute respiratory syndrome OT - Viral replication EDAT- 2017/07/25 06:00 MHDA- 2017/08/30 06:00 PMCR- 2017/07/21 CRDT- 2017/07/25 06:00 PHST- 2017/06/05 00:00 [received] PHST- 2017/07/09 00:00 [revised] PHST- 2017/07/14 00:00 [accepted] PHST- 2017/07/25 06:00 [pubmed] PHST- 2017/08/30 06:00 [medline] PHST- 2017/07/25 06:00 [entrez] PHST- 2017/07/21 00:00 [pmc-release] AID - S0042-6822(17)30238-6 [pii] AID - 10.1016/j.virol.2017.07.019 [doi] PST - ppublish SO - Virology. 2017 Oct;510:165-174. doi: 10.1016/j.virol.2017.07.019. Epub 2017 Jul 21. PMID- 28331093 OWN - NLM STAT- MEDLINE DCOM- 20170727 LR - 20190403 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 91 IP - 11 DP - 2017 Jun 1 TI - Structural Characterization of Human Coronavirus NL63 N Protein. LID - 10.1128/JVI.02503-16 [doi] LID - e02503-16 AB - Coronaviruses are responsible for upper and lower respiratory tract infections in humans. It is estimated that 1 to 10% of the population suffers annually from cold-like symptoms related to infection with human coronavirus NL63 (HCoV-NL63), an alphacoronavirus. The nucleocapsid (N) protein, the major structural component of the capsid, facilitates RNA packing, links the capsid to the envelope, and is also involved in multiple other processes, including viral replication and evasion of the immune system. Although the role of N protein in viral replication is relatively well described, no structural data are currently available regarding the N proteins of alphacoronaviruses. Moreover, our understanding of the mechanisms of RNA binding and nucleocapsid formation remains incomplete. In this study, we solved the crystal structures of the N- and C-terminal domains (NTD, residues 10 to 140, and CTD, residues 221 to 340, respectively) of the N protein of HCoV-NL63, both at a 1.5-Å resolution. Based on our structure of NTD solved here, we proposed and experimentally evaluated a model of RNA binding. The structure of the CTD reveals the mode of N protein dimerization. Overall, this study expands our understanding of the initial steps of N protein-nucleic acid interaction and may facilitate future efforts to control the associated infections.IMPORTANCE Coronaviruses are responsible for the common cold and other respiratory tract infections in humans. According to multiple studies, 1 to 10% of the population is infected each year with HCoV-NL63. Viruses are relatively simple organisms composed of a few proteins and the nucleic acids that carry the information determining their composition. The nucleocapsid (N) protein studied in this work protects the nucleic acid from the environmental factors during virus transmission. This study investigated the structural arrangement of N protein, explaining the first steps of its interaction with nucleic acid at the initial stages of virus structure assembly. The results expand our understanding of coronavirus physiology and may facilitate future efforts to control the associated infections. CI - Copyright © 2017 American Society for Microbiology. FAU - Szelazek, Bozena AU - Szelazek B AD - Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. AD - Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Kabala, Wojciech AU - Kabala W AD - Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Kus, Krzysztof AU - Kus K AD - Instituto Gulbenkian de Ciencia, Oeiras, Portugal. FAU - Zdzalik, Michal AU - Zdzalik M AD - Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Twarda-Clapa, Aleksandra AU - Twarda-Clapa A AD - Faculty of Chemistry, Jagiellonian University, Krakow, Poland. AD - Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Golik, Przemyslaw AU - Golik P AD - Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. AD - Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Burmistrz, Michal AU - Burmistrz M AD - Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Florek, Dominik AU - Florek D AD - Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Wladyka, Benedykt AU - Wladyka B AD - Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. AD - Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Pyrc, Krzysztof AU - Pyrc K AUID- ORCID: 0000-0002-3867-7688 AD - Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. AD - Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Dubin, Grzegorz AU - Dubin G AUID- ORCID: 0000-0002-5535-5760 AD - Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland grzegorz.dubin@uj.edu.pl. AD - Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170512 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Nucleocapsid Proteins) RN - 0 (RNA, Viral) SB - IM MH - Coronavirus NL63, Human/*chemistry/physiology MH - Crystallization MH - Crystallography, X-Ray MH - Humans MH - Models, Molecular MH - Nucleocapsid Proteins/*chemistry/*metabolism MH - Protein Conformation MH - Protein Multimerization MH - RNA, Viral/metabolism MH - Virus Assembly MH - Virus Replication PMC - PMC5432860 OTO - NOTNLM OT - CTD OT - N protein OT - NL63 OT - NTD OT - coronavirus OT - nucleocapsid OT - structure EDAT- 2017/03/24 06:00 MHDA- 2017/07/28 06:00 PMCR- 2017/11/12 CRDT- 2017/03/24 06:00 PHST- 2016/12/28 00:00 [received] PHST- 2017/02/07 00:00 [accepted] PHST- 2017/03/24 06:00 [pubmed] PHST- 2017/07/28 06:00 [medline] PHST- 2017/03/24 06:00 [entrez] PHST- 2017/11/12 00:00 [pmc-release] AID - JVI.02503-16 [pii] AID - 02503-16 [pii] AID - 10.1128/JVI.02503-16 [doi] PST - epublish SO - J Virol. 2017 May 12;91(11):e02503-16. doi: 10.1128/JVI.02503-16. Print 2017 Jun 1. PMID- 27025250 OWN - NLM STAT- MEDLINE DCOM- 20170110 LR - 20220321 IS - 2150-7511 (Electronic) VI - 7 IP - 2 DP - 2016 Mar 29 TI - Middle East Respiratory Syndrome Coronavirus NS4b Protein Inhibits Host RNase L Activation. PG - e00258 LID - 10.1128/mBio.00258-16 [doi] LID - e00258-16 AB - Middle East respiratory syndrome coronavirus (MERS-CoV) is the first highly pathogenic human coronavirus to emerge since severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002. Like many coronaviruses, MERS-CoV carries genes that encode multiple accessory proteins that are not required for replication of the genome but are likely involved in pathogenesis. Evasion of host innate immunity through interferon (IFN) antagonism is a critical component of viral pathogenesis. The IFN-inducible oligoadenylate synthetase (OAS)-RNase L pathway activates upon sensing of viral double-stranded RNA (dsRNA). Activated RNase L cleaves viral and host single-stranded RNA (ssRNA), which leads to translational arrest and subsequent cell death, preventing viral replication and spread. Here we report that MERS-CoV, a lineage CBetacoronavirus, and related bat CoV NS4b accessory proteins have phosphodiesterase (PDE) activity and antagonize OAS-RNase L by enzymatically degrading 2',5'-oligoadenylate (2-5A), activators of RNase L. This is a novel function for NS4b, which has previously been reported to antagonize IFN signaling. NS4b proteins are distinct from lineage ABetacoronavirusPDEs and rotavirus gene-encoded PDEs, in having an amino-terminal nuclear localization signal (NLS) and are localized mostly to the nucleus. However, the expression level of cytoplasmic MERS-CoV NS4b protein is sufficient to prevent activation of RNase L. Finally, this is the first report of an RNase L antagonist expressed by a human or bat coronavirus and provides a specific mechanism by which this occurs. Our findings provide a potential mechanism for evasion of innate immunity by MERS-CoV while also identifying a potential target for therapeutic intervention. IMPORTANCE: Middle East respiratory syndrome coronavirus (MERS-CoV) is the first highly pathogenic human coronavirus to emerge since severe acute respiratory syndrome coronavirus (SARS-CoV). MERS-CoV, like other coronaviruses, carries genes that encode accessory proteins that antagonize the host antiviral response, often the type I interferon response, and contribute to virulence. We found that MERS-CoV NS4b and homologs from related lineage C bat betacoronaviruses BtCoV-SC2013 (SC2013) and BtCoV-HKU5 (HKU5) are members of the 2H-phosphoesterase (2H-PE) enzyme family with phosphodiesterase (PDE) activity. Like murine coronavirus NS2, a previously characterized PDE, MERS NS4b, can antagonize activation of the OAS-RNase L pathway, an interferon-induced potent antiviral activity. Furthermore, MERS-CoV mutants with deletion of genes encoding accessory proteins NS3 to NS5 or NS4b alone or inactivation of the PDE can activate RNase L during infection of Calu-3 cells. Our report may offer a potential target for therapeutic intervention if NS4b proves to be critical to pathogenesis inin vivomodels of MERS-CoV infection. CI - Copyright © 2016 Thornbrough et al. FAU - Thornbrough, Joshua M AU - Thornbrough JM AD - Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Jha, Babal K AU - Jha BK AD - Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA. FAU - Yount, Boyd AU - Yount B AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Goldstein, Stephen A AU - Goldstein SA AD - Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Li, Yize AU - Li Y AD - Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Elliott, Ruth AU - Elliott R AD - Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Sims, Amy C AU - Sims AC AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Baric, Ralph S AU - Baric RS AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Silverman, Robert H AU - Silverman RH AD - Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA. FAU - Weiss, Susan R AU - Weiss SR AUID- ORCID: 0000-0002-8155-4528 AD - Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA weisssr@upenn.edu. LA - eng GR - T32 AI007324/AI/NIAID NIH HHS/United States GR - F32 AI114143/AI/NIAID NIH HHS/United States GR - R21 AI114920/AI/NIAID NIH HHS/United States GR - R01 AI104887/AI/NIAID NIH HHS/United States GR - R21AI114920/AI/NIAID NIH HHS/United States GR - F32AI114143/AI/NIAID NIH HHS/United States GR - U19AI107810/AI/NIAID NIH HHS/United States GR - R01AI104887/AI/NIAID NIH HHS/United States GR - U19 AI107810/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20160329 PL - United States TA - mBio JT - mBio JID - 101519231 RN - 0 (Adenine Nucleotides) RN - 0 (Nuclear Localization Signals) RN - 0 (Oligoribonucleotides) RN - 0 (Viral Nonstructural Proteins) RN - 61172-40-5 (2',5'-oligoadenylate) RN - EC 3.1.- (Endoribonucleases) RN - EC 3.1.26.- (2-5A-dependent ribonuclease) SB - IM MH - Adenine Nucleotides/*metabolism MH - Animals MH - Cricetinae MH - Endoribonucleases/*antagonists & inhibitors MH - *Host-Pathogen Interactions MH - Humans MH - Immune Evasion MH - Mice MH - Middle East Respiratory Syndrome Coronavirus/*physiology MH - Nuclear Localization Signals MH - Oligoribonucleotides/*metabolism MH - Viral Nonstructural Proteins/genetics/*metabolism PMC - PMC4817253 EDAT- 2016/03/31 06:00 MHDA- 2017/01/11 06:00 PMCR- 2016/03/29 CRDT- 2016/03/31 06:00 PHST- 2016/03/31 06:00 [entrez] PHST- 2016/03/31 06:00 [pubmed] PHST- 2017/01/11 06:00 [medline] PHST- 2016/03/29 00:00 [pmc-release] AID - mBio.00258-16 [pii] AID - mBio00258-16 [pii] AID - 10.1128/mBio.00258-16 [doi] PST - epublish SO - mBio. 2016 Mar 29;7(2):e00258. doi: 10.1128/mBio.00258-16. PMID- 25884446 OWN - NLM STAT- MEDLINE DCOM- 20160122 LR - 20181113 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 12 DP - 2015 Apr 11 TI - Diversity of coronavirus in bats from Eastern Thailand. PG - 57 LID - 10.1186/s12985-015-0289-1 [doi] LID - 57 AB - BACKGROUND: Bats are reservoirs for a diverse range of coronaviruses (CoVs), including those closely related to human pathogens such as Severe Acute Respiratory Syndrome (SARS) CoV and Middle East Respiratory Syndrome CoV. There are approximately 139 bat species reported to date in Thailand, of which two are endemic species. Due to the zoonotic potential of CoVs, standardized surveillance efforts to characterize viral diversity in wildlife are imperative. FINDINGS: A total of 626 bats from 19 different bat species were individually sampled from 5 provinces in Eastern Thailand between 2008 and 2013 (84 fecal and 542 rectal swabs). Samples collected (either fresh feces or rectal swabs) were placed directly into RNA stabilization reagent, transported on ice within 24 hours and preserved at -80°C until further analysis. CoV RNA was detected in 47 specimens (7.6%), from 13 different bat species, using broadly reactive consensus PCR primers targeting the RNA-Dependent RNA Polymerase gene designed to detect all CoVs. Thirty seven alphacoronaviruses, nine lineage D betacoronaviruses, and one lineage B betacoronavirus (SARS-CoV related) were identified. Six new bat CoV reservoirs were identified in our study, namely Cynopterus sphinx, Taphozous melanopogon, Hipposideros lekaguli, Rhinolophus shameli, Scotophilus heathii and Megaderma lyra. CONCLUSIONS: CoVs from the same genetic lineage were found in different bat species roosting in similar or different locations. These data suggest that bat CoV lineages are not strictly concordant with their hosts. Our phylogenetic data indicates high diversity and a complex ecology of CoVs in bats sampled from specific areas in eastern regions of Thailand. Further characterization of additional CoV genes may be useful to better describe the CoV divergence. FAU - Wacharapluesadee, Supaporn AU - Wacharapluesadee S AD - World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. spwa@hotmail.com. FAU - Duengkae, Prateep AU - Duengkae P AD - Faculty of Forestry, Kasetsart University, Bangkok, Thailand. prateepd@hotmail.com. FAU - Rodpan, Apaporn AU - Rodpan A AD - World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. wava_38@hotmail.com. FAU - Kaewpom, Thongchai AU - Kaewpom T AD - World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. thongchai_ae@hotmail.com. FAU - Maneeorn, Patarapol AU - Maneeorn P AD - Department of National Parks, Wildlife and Plant Conservation, Bangkok, Thailand. lotterwildlifevet@gmail.com. FAU - Kanchanasaka, Budsabong AU - Kanchanasaka B AD - Department of National Parks, Wildlife and Plant Conservation, Bangkok, Thailand. kbudsabong@yahoo.com. FAU - Yingsakmongkon, Sangchai AU - Yingsakmongkon S AD - World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. sangchai59@hotmail.com. AD - Inter-Department Program of Biomedical Sciences, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand. sangchai59@hotmail.com. FAU - Sittidetboripat, Nuntaporn AU - Sittidetboripat N AD - World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. n.sittidetboripat@gmail.com. FAU - Chareesaen, Chaiyaporn AU - Chareesaen C AD - Department of National Parks, Wildlife and Plant Conservation, Bangkok, Thailand. poom_forest@hotmail.com. FAU - Khlangsap, Nathawat AU - Khlangsap N AD - Faculty of Forestry, Kasetsart University, Bangkok, Thailand. rdispk@ku.ac.th. FAU - Pidthong, Apisit AU - Pidthong A AD - Department of National Parks, Wildlife and Plant Conservation, Bangkok, Thailand. iamapisit@gmail.com. FAU - Leadprathom, Kumron AU - Leadprathom K AD - Royal Forest Department, Bangkok, Thailand. kumron57@gmail.com. FAU - Ghai, Siriporn AU - Ghai S AD - World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. siriporn.ghai@gmail.com. FAU - Epstein, Jonathan H AU - Epstein JH AD - EcoHealth Alliance, New York, USA. epstein@ecohealthalliance.org. FAU - Daszak, Peter AU - Daszak P AD - EcoHealth Alliance, New York, USA. daszak@ecohealthalliance.org. FAU - Olival, Kevin J AU - Olival KJ AD - EcoHealth Alliance, New York, USA. olival@ecohealthalliance.org. FAU - Blair, Patrick J AU - Blair PJ AD - Naval Medical Research Center-Asia, Singapore, Singapore. patricknhrc@gmail.com. FAU - Callahan, Michael V AU - Callahan MV AD - World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. mvcallahan@mgh.harvard.edu. AD - Massachusetts General Hospital, Boston, MA, USA. mvcallahan@mgh.harvard.edu. FAU - Hemachudha, Thiravat AU - Hemachudha T AD - World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. fmedthm@gmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150411 PL - England TA - Virol J JT - Virology journal JID - 101231645 SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronavirus/classification/*genetics/*isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - *Genetic Variation MH - Genome, Viral MH - Humans MH - Molecular Sequence Data MH - Phylogeny MH - Thailand PMC - PMC4416284 EDAT- 2015/04/18 06:00 MHDA- 2016/01/23 06:00 PMCR- 2015/04/11 CRDT- 2015/04/18 06:00 PHST- 2014/10/05 00:00 [received] PHST- 2015/03/25 00:00 [accepted] PHST- 2015/04/18 06:00 [entrez] PHST- 2015/04/18 06:00 [pubmed] PHST- 2016/01/23 06:00 [medline] PHST- 2015/04/11 00:00 [pmc-release] AID - 10.1186/s12985-015-0289-1 [pii] AID - 289 [pii] AID - 10.1186/s12985-015-0289-1 [doi] PST - epublish SO - Virol J. 2015 Apr 11;12:57. doi: 10.1186/s12985-015-0289-1. PMID- 31207129 OWN - NLM STAT- MEDLINE DCOM- 20191219 LR - 20200422 IS - 1865-1682 (Electronic) IS - 1865-1674 (Print) IS - 1865-1674 (Linking) VI - 66 IP - 5 DP - 2019 Sep TI - The re-emerging of SADS-CoV infection in pig herds in Southern China. PG - 2180-2183 LID - 10.1111/tbed.13270 [doi] AB - A new highly virulent swine acute diarrhoea syndrome coronavirus (SADS-CoV) emerged in Guangdong province in 2017 followed by fatal diarrhoea that involved the death of 24,693 piglets. And yet from May 2017 to January 2019, there were no new SADS cases arising in pig herds in Guangdong. In this study, we reported the recent diarrhoea outbreak of SADS-CoV in Southern China on February 2019. Intestinal samples collected from diarrhoeal piglets were detected for common swine virus and confirmed that SADS-CoV was responsible for the diarrhoea case. Meanwhile, serological investigation of sows' sera implied that SADS-CoV has existed in the farm and PEDV antibody may not directly contribute to the amplification of SADS-CoV. Homology and phylogenetic analysis of the whole genome showed that the re-emerging SADS-CoV strain shared high sequence identities with existing SADS-CoV strains and all strains clustered together in Alpha coronavirus. All in all, the report herein emphasized the re-emerging of SADS-CoV and highlights continuous monitoring for this virus. CI - © 2019 Blackwell Verlag GmbH. FAU - Zhou, Ling AU - Zhou L AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Li, Qian N AU - Li QN AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Su, Jiang N AU - Su JN AD - College of Animal Science, South China Agricultural University, Guangzhou, China. FAU - Chen, Gui H AU - Chen GH AD - College of Animal Science, South China Agricultural University, Guangzhou, China. FAU - Wu, Zi X AU - Wu ZX AD - College of Animal Science, South China Agricultural University, Guangzhou, China. FAU - Luo, Ying AU - Luo Y AD - College of Animal Science, South China Agricultural University, Guangzhou, China. FAU - Wu, Rui T AU - Wu RT AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Sun, Yuan AU - Sun Y AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. FAU - Lan, Tian AU - Lan T AD - College of Animal Science, South China Agricultural University, Guangzhou, China. FAU - Ma, Jing Y AU - Ma JY AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, China. LA - eng GR - 2016YFD0501304/National Key Research and Development Program of China/ GR - 201904010433/Science and Technology Program of Guangzhou City of China/ PT - Journal Article DEP - 20190710 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 RN - Swine acute diarrhea syndrome coronavirus SB - IM MH - Alphacoronavirus/genetics/*physiology MH - Animals MH - China/epidemiology MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Diarrhea/epidemiology/veterinary/virology MH - Disease Outbreaks/*veterinary MH - Phylogeny MH - Swine MH - Swine Diseases/*epidemiology/virology PMC - PMC7168562 OTO - NOTNLM OT - SADS-CoV OT - Southern China OT - phylogenetic analysis OT - re-emerging COIS- The authors declare no conflict of interests with any organization. EDAT- 2019/06/18 06:00 MHDA- 2019/12/20 06:00 PMCR- 2020/04/20 CRDT- 2019/06/18 06:00 PHST- 2019/03/25 00:00 [received] PHST- 2019/05/26 00:00 [revised] PHST- 2019/06/11 00:00 [accepted] PHST- 2019/06/18 06:00 [pubmed] PHST- 2019/12/20 06:00 [medline] PHST- 2019/06/18 06:00 [entrez] PHST- 2020/04/20 00:00 [pmc-release] AID - TBED13270 [pii] AID - 10.1111/tbed.13270 [doi] PST - ppublish SO - Transbound Emerg Dis. 2019 Sep;66(5):2180-2183. doi: 10.1111/tbed.13270. Epub 2019 Jul 10. PMID- 31076983 OWN - NLM STAT- MEDLINE DCOM- 20191119 LR - 20221207 IS - 1572-994X (Electronic) IS - 0920-8569 (Print) IS - 0920-8569 (Linking) VI - 55 IP - 4 DP - 2019 Aug TI - Complete genome analysis of a SARS-like bat coronavirus identified in the Republic of Korea. PG - 545-549 LID - 10.1007/s11262-019-01668-w [doi] AB - Bats have been widely known as natural reservoir hosts of zoonotic diseases, such as severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) caused by coronaviruses (CoVs). In the present study, we investigated the whole genomic sequence of a SARS-like bat CoV (16BO133) and found it to be 29,075 nt in length with a 40.9% G+C content. Phylogenetic analysis using amino acid sequences of the ORF 1ab and the spike gene showed that the bat coronavirus strain 16BO133 was grouped with the Beta-CoV lineage B and was closely related to the JTMC15 strain isolated from Rhinolophus ferrumequinum in China. However, 16BO133 was distinctly located in the phylogenetic topology of the human SARS CoV strain (Tor2). Interestingly, 16BO133 showed complete elimination of ORF8 regions induced by a frame shift of the stop codon in ORF7b. The lowest amino acid identity of 16BO133 was identified at the spike region among various ORFs. The spike region of 16BO133 showed 84.7% and 75.2% amino acid identity with Rf1 (SARS-like bat CoV) and Tor2 (human SARS CoV), respectively. In addition, the S gene of 16BO133 was found to contain the amino acid substitution of two critical residues (N479S and T487 V) associated with human infection. In conclusion, we firstly carried out whole genome characterization of the SARS-like bat coronavirus discovered in the Republic of Korea; however, it presumably has no human infectivity. However, continuous surveillance and genomic characterization of coronaviruses from bats are necessary due to potential risks of human infection induced by genetic mutation. FAU - Kim, Yongkwan AU - Kim Y AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, Republic of Korea. AD - Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea. FAU - Son, Kidong AU - Son K AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, Republic of Korea. FAU - Kim, Young-Sik AU - Kim YS AD - Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea. FAU - Lee, Sook-Young AU - Lee SY AD - Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea. FAU - Jheong, Weonhwa AU - Jheong W AD - Environmental Health Research Department, National Institute of Environmental Research, Hwangyeong-ro 42, Seo-gu, Incheon, Republic of Korea. FAU - Oem, Jae-Ku AU - Oem JK AD - Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea. jku0623@jbnu.ac.kr. LA - eng GR - NRF-2018R1D1A1B07041764/National Research Foundation of Korea/ PT - Journal Article DEP - 20190510 PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 SB - IM MH - Animals MH - Betacoronavirus/classification/genetics/*isolation & purification MH - Chiroptera/*virology MH - *Genome, Viral MH - Humans MH - Molecular Typing MH - Phylogeny MH - Republic of Korea MH - Severe acute respiratory syndrome-related coronavirus/genetics MH - Sequence Analysis, Protein MH - Species Specificity MH - Whole Genome Sequencing PMC - PMC7089380 OTO - NOTNLM OT - Bat OT - Frame shift OT - SARS-like coronavirus OT - Whole genome OT - Zoonotic disease COIS- The authors declare that there are no conflicts of interest. EDAT- 2019/05/12 06:00 MHDA- 2019/11/20 06:00 PMCR- 2020/03/23 CRDT- 2019/05/12 06:00 PHST- 2019/02/22 00:00 [received] PHST- 2019/04/30 00:00 [accepted] PHST- 2019/05/12 06:00 [pubmed] PHST- 2019/11/20 06:00 [medline] PHST- 2019/05/12 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s11262-019-01668-w [pii] AID - 1668 [pii] AID - 10.1007/s11262-019-01668-w [doi] PST - ppublish SO - Virus Genes. 2019 Aug;55(4):545-549. doi: 10.1007/s11262-019-01668-w. Epub 2019 May 10. PMID- 28077633 OWN - NLM STAT- MEDLINE DCOM- 20170529 LR - 20190329 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 91 IP - 5 DP - 2017 Mar 1 TI - Surveillance of Bat Coronaviruses in Kenya Identifies Relatives of Human Coronaviruses NL63 and 229E and Their Recombination History. LID - 10.1128/JVI.01953-16 [doi] LID - e01953-16 AB - Bats harbor a large diversity of coronaviruses (CoVs), several of which are related to zoonotic pathogens that cause severe disease in humans. Our screening of bat samples collected in Kenya from 2007 to 2010 not only detected RNA from several novel CoVs but, more significantly, identified sequences that were closely related to human CoVs NL63 and 229E, suggesting that these two human viruses originate from bats. We also demonstrated that human CoV NL63 is a recombinant between NL63-like viruses circulating in Triaenops bats and 229E-like viruses circulating in Hipposideros bats, with the breakpoint located near 5' and 3' ends of the spike (S) protein gene. In addition, two further interspecies recombination events involving the S gene were identified, suggesting that this region may represent a recombination "hot spot" in CoV genomes. Finally, using a combination of phylogenetic and distance-based approaches, we showed that the genetic diversity of bat CoVs is primarily structured by host species and subsequently by geographic distances.IMPORTANCE Understanding the driving forces of cross-species virus transmission is central to understanding the nature of disease emergence. Previous studies have demonstrated that bats are the ultimate reservoir hosts for a number of coronaviruses (CoVs), including ancestors of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and human CoV 229E (HCoV-229E). However, the evolutionary pathways of bat CoVs remain elusive. We provide evidence for natural recombination between distantly related African bat coronaviruses associated with Triaenops afer and Hipposideros sp. bats that resulted in a NL63-like virus, an ancestor of the human pathogen HCoV-NL63. These results suggest that interspecies recombination may play an important role in CoV evolution and the emergence of novel CoVs with zoonotic potential. CI - Copyright © 2017 American Society for Microbiology. FAU - Tao, Ying AU - Tao Y AD - Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Shi, Mang AU - Shi M AD - Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, Australia. FAU - Chommanard, Christina AU - Chommanard C AD - Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Queen, Krista AU - Queen K AD - Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Zhang, Jing AU - Zhang J AD - Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Markotter, Wanda AU - Markotter W AD - Centre for Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa. FAU - Kuzmin, Ivan V AU - Kuzmin IV AD - Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Holmes, Edward C AU - Holmes EC AD - Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, Australia. FAU - Tong, Suxiang AU - Tong S AD - Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA sot1@cdc.gov. LA - eng PT - Journal Article DEP - 20170214 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Viral Proteins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Chiroptera/*virology MH - Conserved Sequence MH - Coronavirus Infections/epidemiology/*veterinary MH - Coronavirus NL63, Human MH - Epidemiological Monitoring MH - Evolution, Molecular MH - Genetic Variation MH - Genome, Viral MH - Kenya/epidemiology MH - Phylogeny MH - Phylogeography MH - Prevalence MH - Recombination, Genetic MH - Respiratory Tract Infections/epidemiology/*veterinary MH - Sequence Analysis, DNA MH - Viral Proteins/chemistry/genetics PMC - PMC5309958 OTO - NOTNLM OT - Africa OT - HCoV-229E OT - HCoV-NL63 OT - bats OT - coronavirus OT - recombination OT - zoonoses EDAT- 2017/01/13 06:00 MHDA- 2017/05/30 06:00 PMCR- 2017/08/14 CRDT- 2017/01/13 06:00 PHST- 2016/09/29 00:00 [received] PHST- 2016/12/04 00:00 [accepted] PHST- 2017/01/13 06:00 [pubmed] PHST- 2017/05/30 06:00 [medline] PHST- 2017/01/13 06:00 [entrez] PHST- 2017/08/14 00:00 [pmc-release] AID - JVI.01953-16 [pii] AID - 01953-16 [pii] AID - 10.1128/JVI.01953-16 [doi] PST - epublish SO - J Virol. 2017 Feb 14;91(5):e01953-16. doi: 10.1128/JVI.01953-16. Print 2017 Mar 1. PMID- 27932284 OWN - NLM STAT- MEDLINE DCOM- 20171103 LR - 20201209 IS - 1567-7257 (Electronic) IS - 1567-1348 (Print) IS - 1567-1348 (Linking) VI - 48 DP - 2017 Mar TI - Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia. PG - 10-18 LID - S1567-1348(16)30513-5 [pii] LID - 10.1016/j.meegid.2016.11.029 [doi] AB - South-East Asia is a hot spot for emerging zoonotic diseases, and bats have been recognized as hosts for a large number of zoonotic viruses such as Severe Acute Respiratory Syndrome (SARS), responsible for acute respiratory syndrome outbreaks. Thus, it is important to expand our knowledge of the presence of viruses in bats which could represent a risk to humans. Coronaviruses (CoVs) have been reported in bat species from Thailand, China, Indonesia, Taiwan and the Philippines. However no such work was conducted in Cambodia or Lao PDR. Between 2010 and 2013, 1965 bats were therefore sampled at interfaces with human populations in these two countries. They were tested for the presence of coronavirus by consensus reverse transcription-PCR assay. A total of 93 samples (4.7%) from 17 genera of bats tested positive. Sequence analysis revealed the presence of potentially 37 and 56 coronavirus belonging to alpha-coronavirus (αCoV) and beta-CoV (βCoV), respectively. The βCoVs group is known to include some coronaviruses highly pathogenic to human, such as SARS-CoV and MERS-CoV. All coronavirus sequences generated from frugivorous bats (family Pteropodidae) (n=55) clustered with other bat βCoVs of lineage D, whereas one coronavirus from Pipistrellus coromandra fell in the lineage C of βCoVs which also includes the MERS-CoV. αCoVs were all detected in various genera of insectivorous bats and clustered with diverse bat αCoV sequences previously published. A closely related strain of PEDV, responsible for severe diarrhea in pigs (PEDV-CoV), was detected in 2 Myotis bats. We highlighted the presence and the high diversity of coronaviruses circulating in bats from Cambodia and Lao PDR. Three new bat genera and species were newly identified as host of coronaviruses, namely Macroglossus sp., Megaerops niphanae and Myotis horsfieldii. CI - Copyright © 2016 Elsevier B.V. All rights reserved. FAU - Lacroix, Audrey AU - Lacroix A AD - Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia. FAU - Duong, Veasna AU - Duong V AD - Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia. FAU - Hul, Vibol AU - Hul V AD - Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia. FAU - San, Sorn AU - San S AD - National Veterinary Research Institute, Ministry of Agriculture Forestry and Fisheries, Cambodia. FAU - Davun, Hull AU - Davun H AD - National Veterinary Research Institute, Ministry of Agriculture Forestry and Fisheries, Cambodia. FAU - Omaliss, Keo AU - Omaliss K AD - Forest Administration, Ministry of Agriculture Forestry and Fisheries, Cambodia. FAU - Chea, Sokha AU - Chea S AD - Wildlife Conservation Society, Cambodia. FAU - Hassanin, Alexandre AU - Hassanin A AD - Muséum national d'Histoire naturelle, Institut de Systématique, Evolution, Biodiversité (ISYEB), UMR 7205 MNHN CNRS UPMC, France. FAU - Theppangna, Watthana AU - Theppangna W AD - National Animal Health Laboratory, Ministry of Agriculture Forestry and Fisheries, Lao Democratic People's Republic. FAU - Silithammavong, Soubanh AU - Silithammavong S AD - Wildlife Conservation Society, Lao Democratic People's Republic; Metabiota Inc., Vientiane, Lao Democratic People's Republic. FAU - Khammavong, Kongsy AU - Khammavong K AD - Wildlife Conservation Society, Lao Democratic People's Republic. FAU - Singhalath, Sinpakone AU - Singhalath S AD - Wildlife Conservation Society, Lao Democratic People's Republic. FAU - Greatorex, Zoe AU - Greatorex Z AD - Wildlife Conservation Society, Lao Democratic People's Republic. FAU - Fine, Amanda E AU - Fine AE AD - Wildlife Conservation Society, Vietnam Program, Hanoi, Vietnam. FAU - Goldstein, Tracey AU - Goldstein T AD - One Health Institute, School of Veterinary Medicine, University of California, Davis, USA. FAU - Olson, Sarah AU - Olson S AD - Wildlife Conservation Society, Wildlife Health Program, Bronx, New York, USA. FAU - Joly, Damien O AU - Joly DO AD - Wildlife Conservation Society, Wildlife Health Program, Bronx, New York, USA; Metabiota Inc., Nanaimo, British Columbia, Canada. FAU - Keatts, Lucy AU - Keatts L AD - Wildlife Conservation Society, Cambodia. FAU - Dussart, Philippe AU - Dussart P AD - Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia. FAU - Afelt, Aneta AU - Afelt A AD - Institute of Physical Geography, Faculty of Geography and Regional Studies, University of Warsaw, Warsaw, Poland. FAU - Frutos, Roger AU - Frutos R AD - Cirad, UMR 17, Cirad-Ird, TA-A17/G, Montpellier, France; Université de Montpellier, IES, UMR 5214, CNRS-UM, Montpellier, France. Electronic address: roger.frutos@ies.univ-montp2.fr. FAU - Buchy, Philippe AU - Buchy P AD - Institut Pasteur du Cambodge, Virology Unit, Phnom Penh, Cambodia; GSK Vaccines R&D, 150 Beach road, # 22-00, 189720, Singapore. Electronic address: buchyphilippe@hotmail.com. LA - eng PT - Journal Article DEP - 20161206 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 RN - 0 (Viral Proteins) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Animals MH - Base Sequence MH - Cambodia MH - Chiroptera/*virology MH - Coronavirus/*genetics MH - Disease Reservoirs MH - Evolution, Molecular MH - Genes, Viral MH - Genetic Variation MH - Laos MH - Phylogeny MH - Phylogeography MH - RNA-Dependent RNA Polymerase/genetics MH - Sequence Analysis, DNA MH - Viral Proteins/genetics PMC - PMC7106194 OTO - NOTNLM OT - Bats OT - Cambodia OT - Coronaviruses OT - Genetic diversity OT - Lao PDR EDAT- 2016/12/10 06:00 MHDA- 2017/11/04 06:00 PMCR- 2016/12/06 CRDT- 2016/12/10 06:00 PHST- 2016/08/01 00:00 [received] PHST- 2016/11/26 00:00 [revised] PHST- 2016/11/26 00:00 [accepted] PHST- 2016/12/10 06:00 [pubmed] PHST- 2017/11/04 06:00 [medline] PHST- 2016/12/10 06:00 [entrez] PHST- 2016/12/06 00:00 [pmc-release] AID - S1567-1348(16)30513-5 [pii] AID - 10.1016/j.meegid.2016.11.029 [doi] PST - ppublish SO - Infect Genet Evol. 2017 Mar;48:10-18. doi: 10.1016/j.meegid.2016.11.029. Epub 2016 Dec 6. PMID- 26190463 OWN - NLM STAT- MEDLINE DCOM- 20160601 LR - 20240328 IS - 1464-3391 (Electronic) IS - 0968-0896 (Print) IS - 0968-0896 (Linking) VI - 23 IP - 17 DP - 2015 Sep 1 TI - Targeting zoonotic viruses: Structure-based inhibition of the 3C-like protease from bat coronavirus HKU4--The likely reservoir host to the human coronavirus that causes Middle East Respiratory Syndrome (MERS). PG - 6036-48 LID - S0968-0896(15)00533-7 [pii] LID - 10.1016/j.bmc.2015.06.039 [doi] AB - The bat coronavirus HKU4 belongs to the same 2c lineage as that of the deadly Middle East Respiratory Syndrome coronavirus (MERS-CoV) and shows high sequence similarity, therefore potentiating a threat to the human population through a zoonotic shift or 'spill over' event. To date, there are no effective vaccines or antiviral treatments available that are capable of limiting the pathogenesis of any human coronaviral infection. An attractive target for the development of anti-coronaviral therapeutics is the 3C-like protease (3CL(pro)), which is essential for the progression of the coronaviral life cycle. Herein, we report the screening results of a small, 230-member peptidomimetic library against HKU4-CoV 3CL(pro) and the identification of 43 peptidomimetic compounds showing good to excellent inhibitory potency of HKU4-CoV 3CL(pro) with IC50 values ranging from low micromolar to sub-micromolar. We established structure-activity relationships (SARs) describing the important ligand-based features required for potent HKU4-CoV 3CL(pro) inhibition and identified a seemingly favored peptidic backbone for HKU4-CoV 3CL(pro) inhibition. To investigate this, a molecular sub-structural analysis of the most potent HKU4-CoV 3CL(pro) inhibitor was accomplished by the synthesis and testing of the lead peptidomimetic inhibitor's sub-structural components, confirming the activity of the favored backbone (22A) identified via SAR analysis. In order to elucidate the structural reasons for such potent HKU4-CoV 3CL(pro) inhibition by the peptidomimetics having the 22A backbone, we determined the X-ray structures of HKU4-CoV 3CL(pro) in complex with three peptidomimetic inhibitors. Sequence alignment of HKU4-CoV 3CL(pro), and two other lineage C Betacoronaviruses 3CL(pro)'s, HKU5-CoV and MERS-CoV 3CL(pro), show that the active site residues of HKU4-CoV 3CL(pro) that participate in inhibitor binding are conserved in HKU5-CoV and MERS-CoV 3CL(pro). Furthermore, we assayed our most potent HKU4-CoV 3CL(pro) inhibitor for inhibition of HKU5-CoV 3CL(pro) and found it to have sub-micromolar inhibitory activity (IC50=0.54±0.03μM). The X-ray structures and SAR analysis reveal critical insights into the structure and inhibition of HKU4-CoV 3CL(pro), providing fundamental knowledge that may be exploited in the development of anti-coronaviral therapeutics for coronaviruses emerging from zoonotic reservoirs. CI - Copyright © 2015 Elsevier Ltd. All rights reserved. FAU - St John, Sarah E AU - St John SE AD - Department of Biological Sciences, Purdue University, West Lafayette, IN, USA; Department of Chemistry, Purdue University, West Lafayette, IN, USA; Centers for Cancer Research & Drug Discovery, Purdue University, West Lafayette, IN, USA. FAU - Tomar, Sakshi AU - Tomar S AD - Department of Biological Sciences, Purdue University, West Lafayette, IN, USA; Centers for Cancer Research & Drug Discovery, Purdue University, West Lafayette, IN, USA. FAU - Stauffer, Shaun R AU - Stauffer SR AD - Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Chemistry, Vanderbilt University Medical Center, Nashville, TN, USA. FAU - Mesecar, Andrew D AU - Mesecar AD AD - Department of Biological Sciences, Purdue University, West Lafayette, IN, USA; Department of Chemistry, Purdue University, West Lafayette, IN, USA; Centers for Cancer Research & Drug Discovery, Purdue University, West Lafayette, IN, USA. Electronic address: amesecar@purdue.edu. LA - eng GR - R01 AI026603/AI/NIAID NIH HHS/United States GR - U54 MH084512/MH/NIMH NIH HHS/United States GR - R01 AI085089/AI/NIAID NIH HHS/United States GR - AI085089/AI/NIAID NIH HHS/United States GR - 1U54MH084659/MH/NIMH NIH HHS/United States GR - P30 CA023168/CA/NCI NIH HHS/United States GR - AI26603/AI/NIAID NIH HHS/United States GR - MH084512/MH/NIMH NIH HHS/United States GR - U54 MH084659/MH/NIMH NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20150619 PL - England TA - Bioorg Med Chem JT - Bioorganic & medicinal chemistry JID - 9413298 RN - 0 (Protease Inhibitors) SB - IM MH - Animals MH - Chiroptera MH - Coronavirus Infections/*enzymology/virology MH - Disease Models, Animal MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*genetics MH - Protease Inhibitors/*therapeutic use MH - Structure-Activity Relationship PMC - PMC5433438 MID - NIHMS705452 OTO - NOTNLM OT - 3C-like protease OT - Broad-spectrum inhibitors OT - Coronavirus OT - HKU4 OT - HKU5 OT - MERS OT - Peptidomimetic compounds OT - Protease inhibitors OT - SARS OT - Zoonotic reservoir EDAT- 2015/07/21 06:00 MHDA- 2016/06/02 06:00 PMCR- 2015/06/19 CRDT- 2015/07/21 06:00 PHST- 2015/04/16 00:00 [received] PHST- 2015/06/02 00:00 [revised] PHST- 2015/06/10 00:00 [accepted] PHST- 2015/07/21 06:00 [entrez] PHST- 2015/07/21 06:00 [pubmed] PHST- 2016/06/02 06:00 [medline] PHST- 2015/06/19 00:00 [pmc-release] AID - S0968-0896(15)00533-7 [pii] AID - 10.1016/j.bmc.2015.06.039 [doi] PST - ppublish SO - Bioorg Med Chem. 2015 Sep 1;23(17):6036-48. doi: 10.1016/j.bmc.2015.06.039. Epub 2015 Jun 19. PMID- 30078094 OWN - NLM STAT- MEDLINE DCOM- 20181211 LR - 20200324 IS - 1572-994X (Electronic) IS - 0920-8569 (Print) IS - 0920-8569 (Linking) VI - 54 IP - 5 DP - 2018 Oct TI - Genomic characterization and pathogenicity of a porcine hemagglutinating encephalomyelitis virus strain isolated in China. PG - 672-683 LID - 10.1007/s11262-018-1591-y [doi] AB - Porcine hemagglutinating encephalomyelitis virus (PHEV) is a member of the genus betacoronavirus within the family coronaviridae, which invades the central nervous system (CNS) via peripheral nervous system and causes encephalomyelitis or vomiting and wasting disease (VWD) in sucking piglets. Up to now, although few complete nucleotide sequences of PHEV have been reported, they are not annotated. This study aimed to illuminate genome characterization, phylogenesis and pathogenicity of the PHEV/2008 strain. The full length of the PHEV/2008 strain genome was 30,684 bp, with a G + C content of 37.27%. The genome included at a minimum of 11 predicted open reading frames (ORFs) flanked by 5' and 3' untranslated regions (UTR) of 211 and 289 nucleotides. The replicase polyproteins pp1a and pp1ab, which had 4382 and 7094 amino acid residues, respectively, were predicted to be cleaved into 16 subunits by two viral proteinases. Phylogenetic analysis based on the complete genome sequence revealed that PHEV/2008 strain was genetically different from other known PHEV types, which represented a novel genotype (GI-1). In addition, we found that PHEV/2008 was neurotropic and highly pathogenic to 4-week-old BALB/c mice. Taken together, this is the first detailed annotated, complete genomic sequence of a new genotype PHEV strain in China. FAU - Shi, Junchao AU - Shi J AUID- ORCID: 0000-0003-0989-0495 AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Zhao, Kui AU - Zhao K AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lu, Huijun AU - Lu H AD - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China. FAU - Li, Zi AU - Li Z AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lv, Xiaoling AU - Lv X AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lan, Yungang AU - Lan Y AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Guan, Jiyu AU - Guan J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - He, Wenqi AU - He W AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Gao, Feng AU - Gao F AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. gaofeng@jlu.edu.cn. LA - eng GR - 2016YFD0500102/the National Key Research and Development Program of China/ GR - 2016YFD0500707/the National Key Research and Development Program of China/ GR - 31772704/the National Natural Science Foundation of China/ GR - 31672519/the National Natural Science Foundation of China/ GR - 31602018/the National Natural Science Foundation of China/ GR - 20180101270JC/the Scientific and Technological Project of Jilin Province/ GR - 20170204033NY/the Scientific and Technological Project of Jilin Province/ GR - 20160520033JH/the Scientific and Technological Project of Jilin Province/ PT - Journal Article DEP - 20180804 PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 RN - 0 (DNA, Viral) RN - 0 (Viral Proteins) SB - IM MH - Animals MH - Betacoronavirus 1/*genetics/isolation & purification/*pathogenicity MH - China MH - Cloning, Molecular MH - Coronavirus Infections/virology MH - DNA, Viral MH - Female MH - *Genome, Viral MH - Humans MH - Mice, Inbred BALB C MH - Molecular Typing MH - Open Reading Frames MH - Phylogeny MH - Real-Time Polymerase Chain Reaction MH - Sequence Analysis, DNA MH - Swine/virology MH - Viral Proteins/chemistry/genetics PMC - PMC7089186 OTO - NOTNLM OT - Complete genome sequence OT - Detailed annotation OT - Expression strategy OT - PHEV OT - Pathogenicity OT - Phylogenetic analysis COIS- The authors declare that they have no conflict of interest. EDAT- 2018/08/06 06:00 MHDA- 2018/12/12 06:00 PMCR- 2020/03/23 CRDT- 2018/08/06 06:00 PHST- 2018/06/03 00:00 [received] PHST- 2018/07/19 00:00 [accepted] PHST- 2018/08/06 06:00 [pubmed] PHST- 2018/12/12 06:00 [medline] PHST- 2018/08/06 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s11262-018-1591-y [pii] AID - 1591 [pii] AID - 10.1007/s11262-018-1591-y [doi] PST - ppublish SO - Virus Genes. 2018 Oct;54(5):672-683. doi: 10.1007/s11262-018-1591-y. Epub 2018 Aug 4. PMID- 26719272 OWN - NLM STAT- MEDLINE DCOM- 20160722 LR - 20221207 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 90 IP - 6 DP - 2015 Dec 30 TI - Isolation and Characterization of a Novel Bat Coronavirus Closely Related to the Direct Progenitor of Severe Acute Respiratory Syndrome Coronavirus. PG - 3253-6 LID - 10.1128/JVI.02582-15 [doi] AB - We report the isolation and characterization of a novel bat coronavirus which is much closer to the severe acute respiratory syndrome coronavirus (SARS-CoV) in genomic sequence than others previously reported, particularly in its S gene. Cell entry and susceptibility studies indicated that this virus can use ACE2 as a receptor and infect animal and human cell lines. Our results provide further evidence of the bat origin of the SARS-CoV and highlight the likelihood of future bat coronavirus emergence in humans. CI - Copyright © 2016, American Society for Microbiology. All Rights Reserved. FAU - Yang, Xing-Lou AU - Yang XL AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Hu, Ben AU - Hu B AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Wang, Bo AU - Wang B AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Wang, Mei-Niang AU - Wang MN AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zhang, Qian AU - Zhang Q AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zhang, Wei AU - Zhang W AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Wu, Li-Jun AU - Wu LJ AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Ge, Xing-Yi AU - Ge XY AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zhang, Yun-Zhi AU - Zhang YZ AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, China. FAU - Daszak, Peter AU - Daszak P AD - EcoHealth Alliance, New York, New York, USA. FAU - Wang, Lin-Fa AU - Wang LF AD - Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore, Singapore. FAU - Shi, Zheng-Li AU - Shi ZL AUID- ORCID: 0000-0001-8089-163X AD - Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China zlshi@wh.iov.cn. LA - eng GR - R01 AI110964/AI/NIAID NIH HHS/United States GR - R01AI110964/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20151230 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (RNA, Viral) RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.15.1 (Peptidyl-Dipeptidase A) RN - EC 3.4.17.23 (ACE2 protein, human) RN - EC 3.4.17.23 (Angiotensin-Converting Enzyme 2) SB - IM MH - Angiotensin-Converting Enzyme 2 MH - Animals MH - Cell Line MH - Chiroptera/*virology MH - Coronavirus/*classification/genetics/*isolation & purification/physiology MH - Humans MH - Molecular Sequence Data MH - Peptidyl-Dipeptidase A/metabolism MH - *Phylogeny MH - RNA, Viral/genetics MH - Receptors, Virus/metabolism MH - Severe acute respiratory syndrome-related coronavirus/genetics MH - Sequence Analysis, DNA MH - Sequence Homology MH - Spike Glycoprotein, Coronavirus/genetics MH - Virus Attachment PMC - PMC4810638 EDAT- 2016/01/01 06:00 MHDA- 2016/07/23 06:00 PMCR- 2016/08/26 CRDT- 2016/01/01 06:00 PHST- 2015/10/13 00:00 [received] PHST- 2015/12/21 00:00 [accepted] PHST- 2016/01/01 06:00 [entrez] PHST- 2016/01/01 06:00 [pubmed] PHST- 2016/07/23 06:00 [medline] PHST- 2016/08/26 00:00 [pmc-release] AID - JVI.02582-15 [pii] AID - 02582-15 [pii] AID - 10.1128/JVI.02582-15 [doi] PST - epublish SO - J Virol. 2015 Dec 30;90(6):3253-6. doi: 10.1128/JVI.02582-15. PMID- 29463282 OWN - NLM STAT- MEDLINE DCOM- 20180918 LR - 20181113 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 15 IP - 1 DP - 2018 Feb 20 TI - Longitudinal study of age-specific pattern of coronavirus infection in Lyle's flying fox (Pteropus lylei) in Thailand. PG - 38 LID - 10.1186/s12985-018-0950-6 [doi] LID - 38 AB - BACKGROUND: Bats are natural reservoirs for several highly pathogenic and novel viruses including coronaviruses (CoVs) (mainly Alphacoronavirus and Betacoronavirus). Lyle's flying fox (Pteropus lylei)'s roosts and foraging sites are usually in the proximity to humans and animals. Knowledge about age-specific pattern of CoV infection in P. lylei, prevalence, and viral shedding at roosts and foraging sites may have an impact on infection-age-structure model to control CoV outbreak. METHODS: P. lylei bats were captured monthly during January-December 2012 for detection of CoV at three areas in Chonburi province; two human dwellings, S1 and S2, where few fruit trees were located with an open pig farm, 0.6 km and 5.5 km away from the bat roost, S3. Nested RT-PCR of RNA-dependent RNA polymerase (RdRp) gene from rectal swabs was used for CoV detection. The strain of CoV was confirmed by sequencing and phylogenetic analysis. RESULTS: CoV infection was found in both juveniles and adult bats between May and October (January, in adults only and April, in juveniles only). Of total rectal swab positives (68/367, 18.5%), ratio was higher in bats captured at S1 (11/44, 25.0%) and S2 (35/99, 35.4%) foraging sites than at roost (S3) (22/224, 9.8%). Juveniles (forearm length ≤ 136 mm) were found with more CoV infection than adults at all three sites; S1 (9/24, 37.5% vs 2/20, 10%), S2 (22/49, 44.9% vs 13/50, 26.0%), and S3 (10/30, 33.3% vs 12/194, 6.2%). The average BCI of CoV infected bats was significantly lower than uninfected bats. No gender difference related to infection was found at the sites. Phylogenetic analysis of conserved RdRp gene revealed that the detected CoVs belonged to group D betacoronavirus (n = 64) and alphacoronavirus (n = 4). CONCLUSIONS: The fact that CoV infection and shedding was found in more juvenile than adult bats may suggest transmission from mother during peripartum period. Whether viral reactivation during parturition period or stress is responsible in maintaining transmission in the bat colony needs to be explored. FAU - Wacharapluesadee, Supaporn AU - Wacharapluesadee S AUID- ORCID: 0000-0001-7636-1900 AD - Thai Red Cross Emerging Infectious Diseases - Health Science Centre, World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, Chulalongkorn Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. spwa@hotmail.com. FAU - Duengkae, Prateep AU - Duengkae P AD - Faculty of Forestry, Kasetsart University, Bangkok, Thailand. FAU - Chaiyes, Aingorn AU - Chaiyes A AD - Faculty of Forestry, Kasetsart University, Bangkok, Thailand. FAU - Kaewpom, Thongchai AU - Kaewpom T AD - Thai Red Cross Emerging Infectious Diseases - Health Science Centre, World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, Chulalongkorn Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. FAU - Rodpan, Apaporn AU - Rodpan A AD - Thai Red Cross Emerging Infectious Diseases - Health Science Centre, World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, Chulalongkorn Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. FAU - Yingsakmongkon, Sangchai AU - Yingsakmongkon S AD - Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand. FAU - Petcharat, Sininat AU - Petcharat S AD - Thai Red Cross Emerging Infectious Diseases - Health Science Centre, World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, Chulalongkorn Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. FAU - Phengsakul, Patcharakiti AU - Phengsakul P AD - Faculty of Forestry, Kasetsart University, Bangkok, Thailand. FAU - Maneeorn, Pattarapol AU - Maneeorn P AD - Department of National Parks, Wildlife and Plant Conservation, Bangkok, Thailand. FAU - Hemachudha, Thiravat AU - Hemachudha T AD - Thai Red Cross Emerging Infectious Diseases - Health Science Centre, World Health Organization Collaborating Centre for Research and Training on Viral Zoonoses, Chulalongkorn Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180220 PL - England TA - Virol J JT - Virology journal JID - 101231645 RN - 0 (RNA, Viral) SB - IM MH - Age Factors MH - Animal Diseases/*epidemiology/*virology MH - Animals MH - Chiroptera/*virology MH - *Coronavirus/genetics MH - Coronavirus Infections/*veterinary MH - Female MH - Genome, Viral MH - Longitudinal Studies MH - Male MH - Phylogeny MH - Prevalence MH - RNA, Viral MH - Thailand/epidemiology MH - Virus Shedding PMC - PMC5819653 OTO - NOTNLM OT - Chiroptera OT - Coronavirus OT - Pteropus OT - Thailand COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Bat specimens were collected with permission from the Department of National Parks, Wildlife and Plant Conservation (No. 0909.204/2686) and the Animal Use Protocol No.1473001 approved by Chulalongkorn University Animal Care and Use Committee. CONSENT FOR PUBLICATION: Not Applicable. COMPETING INTERESTS: The authors claim no conflict of interest in the publication of this information. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/02/22 06:00 MHDA- 2018/09/19 06:00 PMCR- 2018/02/20 CRDT- 2018/02/22 06:00 PHST- 2017/11/21 00:00 [received] PHST- 2018/02/15 00:00 [accepted] PHST- 2018/02/22 06:00 [entrez] PHST- 2018/02/22 06:00 [pubmed] PHST- 2018/09/19 06:00 [medline] PHST- 2018/02/20 00:00 [pmc-release] AID - 10.1186/s12985-018-0950-6 [pii] AID - 950 [pii] AID - 10.1186/s12985-018-0950-6 [doi] PST - epublish SO - Virol J. 2018 Feb 20;15(1):38. doi: 10.1186/s12985-018-0950-6. PMID- 28003490 OWN - NLM STAT- MEDLINE DCOM- 20170529 LR - 20200402 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 91 IP - 5 DP - 2017 Mar 1 TI - Lineage A Betacoronavirus NS2 Proteins and the Homologous Torovirus Berne pp1a Carboxy-Terminal Domain Are Phosphodiesterases That Antagonize Activation of RNase L. LID - 10.1128/JVI.02201-16 [doi] LID - e02201-16 AB - Viruses in the family Coronaviridae, within the order Nidovirales, are etiologic agents of a range of human and animal diseases, including both mild and severe respiratory diseases in humans. These viruses encode conserved replicase and structural proteins as well as more diverse accessory proteins, encoded in the 3' ends of their genomes, that often act as host cell antagonists. We previously showed that 2',5'-phosphodiesterases (2',5'-PDEs) encoded by the prototypical Betacoronavirus, mouse hepatitis virus (MHV), and by Middle East respiratory syndrome-associated coronavirus antagonize the oligoadenylate-RNase L (OAS-RNase L) pathway. Here we report that additional coronavirus superfamily members, including lineage A betacoronaviruses and toroviruses infecting both humans and animals, encode 2',5'-PDEs capable of antagonizing RNase L. We used a chimeric MHV system (MHV(Mut)) in which exogenous PDEs were expressed from an MHV backbone lacking the gene for a functional NS2 protein, the endogenous RNase L antagonist. With this system, we found that 2',5'-PDEs encoded by the human coronavirus HCoV-OC43 (OC43; an agent of the common cold), human enteric coronavirus (HECoV), equine coronavirus (ECoV), and equine torovirus Berne (BEV) are enzymatically active, rescue replication of MHV(Mut) in bone marrow-derived macrophages, and inhibit RNase L-mediated rRNA degradation in these cells. Additionally, PDEs encoded by OC43 and BEV rescue MHV(Mut) replication and restore pathogenesis in wild-type (WT) B6 mice. This finding expands the range of viruses known to encode antagonists of the potent OAS-RNase L antiviral pathway, highlighting its importance in a range of species as well as the selective pressures exerted on viruses to antagonize it.IMPORTANCE Viruses in the family Coronaviridae include important human and animal pathogens, including the recently emerged viruses severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and Middle East respiratory syndrome-associated coronavirus (MERS-CoV). We showed previously that two viruses within the genus Betacoronavirus, mouse hepatitis virus (MHV) and MERS-CoV, encode 2',5'-phosphodiesterases (2',5'-PDEs) that antagonize the OAS-RNase L pathway, and we report here that these proteins are furthermore conserved among additional coronavirus superfamily members, including lineage A betacoronaviruses and toroviruses, suggesting that they may play critical roles in pathogenesis. As there are no licensed vaccines or effective antivirals against human coronaviruses and few against those infecting animals, identifying viral proteins contributing to virulence can inform therapeutic development. Thus, this work demonstrates that a potent antagonist of host antiviral defenses is encoded by multiple and diverse viruses within the family Coronaviridae, presenting a possible broad-spectrum therapeutic target. CI - Copyright © 2017 American Society for Microbiology. FAU - Goldstein, Stephen A AU - Goldstein SA AD - Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Thornbrough, Joshua M AU - Thornbrough JM AD - Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Zhang, Rong AU - Zhang R AD - Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Jha, Babal K AU - Jha BK AUID- ORCID: 0000-0002-7660-5255 AD - Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA. FAU - Li, Yize AU - Li Y AD - Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Elliott, Ruth AU - Elliott R AD - Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Quiroz-Figueroa, Katherine AU - Quiroz-Figueroa K AD - Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Chen, Annie I AU - Chen AI AD - Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Silverman, Robert H AU - Silverman RH AD - Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA. FAU - Weiss, Susan R AU - Weiss SR AUID- ORCID: 0000-0002-8155-4528 AD - Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA weisssr@upenn.edu. LA - eng GR - F32 AI114143/AI/NIAID NIH HHS/United States GR - R01 AI104887/AI/NIAID NIH HHS/United States GR - R21 AI114920/AI/NIAID NIH HHS/United States GR - T32 AI007324/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20170214 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Adenine Nucleotides) RN - 0 (Oligoribonucleotides) RN - 0 (Viral Nonstructural Proteins) RN - 61172-40-5 (2',5'-oligoadenylate) RN - EC 3.1.- (Endoribonucleases) RN - EC 3.1.26.- (2-5A-dependent ribonuclease) RN - EC 3.1.4.- (Phosphoric Diester Hydrolases) SB - IM MH - Adenine Nucleotides/chemistry MH - Amino Acid Sequence MH - Animals MH - Catalytic Domain MH - Cell Line MH - Conserved Sequence MH - Cricetinae MH - Endoribonucleases/*metabolism MH - Enzyme Activation MH - Macrophages/virology MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - Middle East Respiratory Syndrome Coronavirus/*enzymology MH - Murine hepatitis virus/*enzymology MH - Oligoribonucleotides/chemistry MH - Phosphoric Diester Hydrolases/chemistry/*physiology MH - Torovirus/*enzymology MH - Viral Nonstructural Proteins/chemistry/*physiology MH - Virus Replication PMC - PMC5309944 OTO - NOTNLM OT - RNase L OT - interferon antagonist OT - lineage A coronavirus OT - oligoadenylate synthetase OT - phosphodiesterase OT - torovirus EDAT- 2016/12/23 06:00 MHDA- 2017/05/30 06:00 PMCR- 2017/08/14 CRDT- 2016/12/23 06:00 PHST- 2016/11/08 00:00 [received] PHST- 2016/12/13 00:00 [accepted] PHST- 2016/12/23 06:00 [pubmed] PHST- 2017/05/30 06:00 [medline] PHST- 2016/12/23 06:00 [entrez] PHST- 2017/08/14 00:00 [pmc-release] AID - JVI.02201-16 [pii] AID - 02201-16 [pii] AID - 10.1128/JVI.02201-16 [doi] PST - epublish SO - J Virol. 2017 Feb 14;91(5):e02201-16. doi: 10.1128/JVI.02201-16. Print 2017 Mar 1. PMID- 28293544 OWN - NLM STAT- MEDLINE DCOM- 20171201 LR - 20181113 IS - 2235-2988 (Electronic) IS - 2235-2988 (Linking) VI - 7 DP - 2017 TI - Induction of Atypical Autophagy by Porcine Hemagglutinating Encephalomyelitis Virus Contributes to Viral Replication. PG - 56 LID - 10.3389/fcimb.2017.00056 [doi] LID - 56 AB - Autophagy is a basic biological metabolic process involving in intracellular membrane transport pathways that recycle cellular components and eliminate intracellular microorganisms within the lysosome. Autophagy also plays an important part in virus infection and propagation. However, some pathogens, including viruses, have evolved unique trick to escape or exploit autophagy. This study explores the mechanism of autophagy induction by porcine hemagglutinating encephalomyelitis virus (PHEV) in Neuro-2a cells, and examines the role of autophagy in PHEV replication. PHEV triggered autophagy in Neuro-2a cells is dependent on the presence of bulk double- or single-membrane vacuoles, the accumulation of GFP-LC3 fluorescent dots, and the LC3 lipidation. In addition, PHEV induced an incomplete autophagic effect because the degradation level of p62 did not change in PHEV-infected cells. Further validation was captured using LysoTracker and lysosome-associated membrane protein by indirect immunofluorescence labeling in PHEV-infected cells. We also investigated the change in viral replication by pharmacological experiments with the autophagy inducer rapamycin or the autophagy inhibitor 3-MA, and the lysosomal inhibitor chloroquine (CQ). Suppression of autophagy by 3-MA increased viral replication, compared with the mock treatment, while promoting of autophagy by rapamycin reduced PHEV replication. CQ treatment enhanced the LC3 lipidation in PHEV-infected Neuro-2a cells but lowered PHEV replication. These results show that PHEV infection induces atypical autophagy and causes the appearance of autophagosomes but blocks the fusion with lysosomes, which is necessary for the replication of PHEV in nerve cells. FAU - Ding, Ning AU - Ding N AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, China. FAU - Zhao, Kui AU - Zhao K AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, China. FAU - Lan, Yungang AU - Lan Y AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, China. FAU - Li, Zi AU - Li Z AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, China. FAU - Lv, Xiaoling AU - Lv X AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, China. FAU - Su, Jingjing AU - Su J AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, China. FAU - Lu, Huijun AU - Lu H AD - Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University Changchun, China. FAU - Gao, Feng AU - Gao F AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, China. FAU - He, Wenqi AU - He W AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University Changchun, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170228 PL - Switzerland TA - Front Cell Infect Microbiol JT - Frontiers in cellular and infection microbiology JID - 101585359 RN - 0 (Membrane Proteins) RN - 5142-23-4 (3-methyladenine) RN - 886U3H6UFF (Chloroquine) RN - JAC85A2161 (Adenine) RN - W36ZG6FT64 (Sirolimus) SB - IM MH - Adenine/analogs & derivatives/pharmacology MH - Animals MH - Autophagosomes/metabolism/ultrastructure/*virology MH - Autophagy/drug effects/*physiology MH - Betacoronavirus 1/pathogenicity/*physiology MH - Cell Line MH - Cell Survival MH - Chloroquine/pharmacology MH - Coronavirus Infections/virology MH - Lysosomes/metabolism/virology MH - Membrane Proteins/metabolism MH - Mice MH - Sirolimus/pharmacology MH - Vacuoles/metabolism MH - Virus Replication/drug effects/*physiology PMC - PMC5328988 OTO - NOTNLM OT - LC3 OT - atypical autophagy OT - autophagic flux OT - neuro-2a cells OT - neurotropic virus OT - porcine hemagglutinating encephalomyelitis virus OT - virus replication EDAT- 2017/03/16 06:00 MHDA- 2017/12/02 06:00 PMCR- 2017/01/01 CRDT- 2017/03/16 06:00 PHST- 2016/12/10 00:00 [received] PHST- 2017/02/13 00:00 [accepted] PHST- 2017/03/16 06:00 [entrez] PHST- 2017/03/16 06:00 [pubmed] PHST- 2017/12/02 06:00 [medline] PHST- 2017/01/01 00:00 [pmc-release] AID - 10.3389/fcimb.2017.00056 [doi] PST - epublish SO - Front Cell Infect Microbiol. 2017 Feb 28;7:56. doi: 10.3389/fcimb.2017.00056. eCollection 2017. PMID- 27928918 OWN - NLM STAT- MEDLINE DCOM- 20170627 LR - 20191113 IS - 0001-723X (Print) IS - 0001-723X (Linking) VI - 60 IP - 4 DP - 2016 TI - Characterization and phylogenetic analysis of new bat astroviruses detected in Gabon, Central Africa. PG - 386-392 AB - Astroviruses are emerging RNA viruses that cause enteropathogenic infections in humans and in other mammals. The identification of astroviruses in a wide range of animals highlights the zoonotic importance of these viruses. Bats can harbor many different viruses, among which some are highly pathogenic for humans (for instance, Nipah, Ebola and SARS coronavirus), and also several astroviruses. As some RNA viruses can be directly transmitted from bats to humans, it is crucial to collect data about their frequency, genetic diversity and phylogenetic characterization. In this study, we report the molecular identification of 44 new astroviruses (with a detection rate of 4.5%) in 962 apparently healthy bats that belong to five different species and that were captured in different caves in North-East Gabon, Central Africa. Our results show that bat astroviruses form a group that is genetically distinct from astroviruses infecting other mammals. Moreover, these astroviruses showed an important genetic diversity and low host restriction in bat species. FAU - Rougeron, V AU - Rougeron V FAU - Suquet, E AU - Suquet E FAU - Maganga, G D AU - Maganga GD FAU - Jiolle, D AU - Jiolle D FAU - Mombo, I M AU - Mombo IM FAU - Bourgarel, M AU - Bourgarel M FAU - Motsch, P AU - Motsch P FAU - Arnathau, C AU - Arnathau C FAU - Durand, P AU - Durand P FAU - Drexler, F AU - Drexler F FAU - Drosten, C AU - Drosten C FAU - Renaud, F AU - Renaud F FAU - Prugnolle, F AU - Prugnolle F FAU - Leroy, E M AU - Leroy EM LA - eng PT - Journal Article PL - Switzerland TA - Acta Virol JT - Acta virologica JID - 0370401 SB - IM MH - Animals MH - Astroviridae/classification/*genetics/isolation & purification MH - Astroviridae Infections/*veterinary/virology MH - Chiroptera/*virology MH - Gabon MH - Genetic Variation MH - Humans MH - Mammals/virology MH - *Phylogeny OTO - NOTNLM OT - bat; astroviruses; Gabon; host restriction; genetic diversity. EDAT- 2016/12/09 06:00 MHDA- 2017/06/28 06:00 CRDT- 2016/12/09 06:00 PHST- 2016/12/09 06:00 [entrez] PHST- 2016/12/09 06:00 [pubmed] PHST- 2017/06/28 06:00 [medline] AID - 10.4149/av_2016_04_386 [doi] PST - ppublish SO - Acta Virol. 2016;60(4):386-392. doi: 10.4149/av_2016_04_386. PMID- 27164099 OWN - NLM STAT- MEDLINE DCOM- 20170309 LR - 20181113 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 17 IP - 5 DP - 2016 May 7 TI - Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23. LID - 10.3390/ijms17050691 [doi] LID - 691 AB - Recently, we reported the discovery of a dromedary camel coronavirus UAE-HKU23 (DcCoV UAE-HKU23) from dromedaries in the Middle East. In this study, DcCoV UAE-HKU23 was successfully isolated in two of the 14 dromedary fecal samples using HRT-18G cells, with cytopathic effects observed five days after inoculation. Northern blot analysis revealed at least seven distinct RNA species, corresponding to predicted subgenomic mRNAs and confirming the core sequence of transcription regulatory sequence motifs as 5'-UCUAAAC-3' as we predicted previously. Antibodies against DcCoV UAE-HKU23 were detected in 58 (98.3%) and 59 (100%) of the 59 dromedary sera by immunofluorescence and neutralization antibody tests, respectively. There was significant correlation between the antibody titers determined by immunofluorescence and neutralization assays (Pearson coefficient = 0.525, p < 0.0001). Immunization of mice using recombinant N proteins of DcCoV UAE-HKU23 and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, and heat-inactivated DcCoV UAE-HKU23 showed minimal cross-antigenicity between DcCoV UAE-HKU23 and MERS-CoV by Western blot and neutralization antibody assays. Codon usage and genetic distance analysis of RdRp, S and N genes showed that the 14 strains of DcCoV UAE-HKU23 formed a distinct cluster, separated from those of other closely related members of Betacoronavirus 1, including alpaca CoV, confirming that DcCoV UAE-HKU23 is a novel member of Betacoronavirus 1. FAU - Woo, Patrick C Y AU - Woo PC AD - State Key Laboratory of Emerging Infectious Diseases, the University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. AD - Research Centre of Infection and Immunology, the University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. AD - Carol Yu Centre for Infection, the University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310006, China. pcywoo@hku.hk. FAU - Lau, Susanna K P AU - Lau SK AD - State Key Laboratory of Emerging Infectious Diseases, the University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. AD - Research Centre of Infection and Immunology, the University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. AD - Carol Yu Centre for Infection, the University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310006, China. skplau@hku.hk. FAU - Fan, Rachel Y Y AU - Fan RY AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. rachelfyy2004@yahoo.com.hk. FAU - Lau, Candy C Y AU - Lau CC AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. candylaucy@gmail.com. FAU - Wong, Emily Y M AU - Wong EY AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. emilyhk2811@gmail.com. FAU - Joseph, Sunitha AU - Joseph S AD - Central Veterinary Research Laboratory, Dubai, UAE. sjoseph@cvrl.ae. FAU - Tsang, Alan K L AU - Tsang AK AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. alantsangmb@gmail.com. FAU - Wernery, Renate AU - Wernery R AD - Central Veterinary Research Laboratory, Dubai, UAE. wernery@cvrl.ae. FAU - Yip, Cyril C Y AU - Yip CC AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. cyrilyip@gmail.com. FAU - Tsang, Chi-Ching AU - Tsang CC AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. microbioct@connect.hku.hk. FAU - Wernery, Ulrich AU - Wernery U AD - Central Veterinary Research Laboratory, Dubai, UAE. cvrl@cvrl.ae. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory of Emerging Infectious Diseases, the University of Hong Kong, Pokfulam, Hong Kong. kyyuen@hku.hk. AD - Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. kyyuen@hku.hk. AD - Research Centre of Infection and Immunology, the University of Hong Kong, Pokfulam, Hong Kong. kyyuen@hku.hk. AD - Carol Yu Centre for Infection, the University of Hong Kong, Pokfulam, Hong Kong. kyyuen@hku.hk. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310006, China. kyyuen@hku.hk. LA - eng PT - Journal Article DEP - 20160507 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Animals MH - Camelus/*virology MH - Cell Line, Tumor MH - *Cross Reactions MH - Genes, Viral MH - Humans MH - Mice MH - Middle East Respiratory Syndrome Coronavirus/classification/genetics/*immunology MH - Phylogeny PMC - PMC4881517 OTO - NOTNLM OT - characterization OT - coronavirus OT - dromedary camel OT - isolation EDAT- 2016/05/11 06:00 MHDA- 2017/03/10 06:00 PMCR- 2016/05/01 CRDT- 2016/05/11 06:00 PHST- 2016/03/17 00:00 [received] PHST- 2016/04/21 00:00 [revised] PHST- 2016/04/25 00:00 [accepted] PHST- 2016/05/11 06:00 [entrez] PHST- 2016/05/11 06:00 [pubmed] PHST- 2017/03/10 06:00 [medline] PHST- 2016/05/01 00:00 [pmc-release] AID - ijms17050691 [pii] AID - ijms-17-00691 [pii] AID - 10.3390/ijms17050691 [doi] PST - epublish SO - Int J Mol Sci. 2016 May 7;17(5):691. doi: 10.3390/ijms17050691. PMID- 30676269 OWN - NLM STAT- MEDLINE DCOM- 20190812 LR - 20220129 IS - 1557-7759 (Electronic) IS - 1530-3667 (Print) IS - 1530-3667 (Linking) VI - 19 IP - 3 DP - 2019 Mar TI - What Have We Learned About Middle East Respiratory Syndrome Coronavirus Emergence in Humans? A Systematic Literature Review. PG - 174-192 LID - 10.1089/vbz.2017.2191 [doi] AB - BACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV) was first identified in humans in 2012. A systematic literature review was conducted to synthesize current knowledge and identify critical knowledge gaps. MATERIALS AND METHODS: We conducted a systematic review on MERS-CoV using PRISMA guidelines. We identified 407 relevant, peer-reviewed publications and selected 208 of these based on their contributions to four key areas: virology; clinical characteristics, outcomes, therapeutic and preventive options; epidemiology and transmission; and animal interface and the search for natural hosts of MERS-CoV. RESULTS: Dipeptidyl peptidase 4 (DPP4/CD26) was identified as the human receptor for MERS-CoV, and a variety of molecular and serological assays developed. Dromedary camels remain the only documented zoonotic source of human infection, but MERS-like CoVs have been detected in bat species globally, as well as in dromedary camels throughout the Middle East and Africa. However, despite evidence of camel-to-human MERS-CoV transmission and cases apparently related to camel contact, the source of many primary cases remains unknown. There have been sustained health care-associated human outbreaks in Saudi Arabia and South Korea, the latter originating from one traveler returning from the Middle East. Transmission mechanisms are poorly understood; for health care, this may include environmental contamination. Various potential therapeutics have been identified, but not yet evaluated in human clinical trials. At least one candidate vaccine has progressed to Phase I trials. CONCLUSIONS: There has been substantial MERS-CoV research since 2012, but significant knowledge gaps persist, especially in epidemiology and natural history of the infection. There have been few rigorous studies of baseline prevalence, transmission, and spectrum of disease. Terms such as "camel exposure" and the epidemiological relationships of cases should be clearly defined and standardized. We strongly recommend a shared and accessible registry or database. Coronaviruses will likely continue to emerge, arguing for a unified "One Health" approach. FAU - Dawson, Patrick AU - Dawson P AD - 1 Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York. FAU - Malik, Mamunur Rahman AU - Malik MR AD - 2 Infectious Hazard Management, Department of Health Emergency, World Health Organization Eastern Mediterranean Regional Office (WHO/EMRO), Cairo, Egypt. FAU - Parvez, Faruque AU - Parvez F AD - 3 Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, New York. FAU - Morse, Stephen S AU - Morse SS AD - 1 Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York. LA - eng GR - 001/WHO_/World Health Organization/International PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Systematic Review DEP - 20190124 PL - United States TA - Vector Borne Zoonotic Dis JT - Vector borne and zoonotic diseases (Larchmont, N.Y.) JID - 100965525 MH - Africa/epidemiology MH - Animals MH - Communicable Diseases, Emerging/*virology MH - Coronavirus Infections/epidemiology/*virology MH - Disease Outbreaks MH - Humans MH - Middle East/epidemiology MH - Middle East Respiratory Syndrome Coronavirus/*physiology MH - Zoonoses PMC - PMC6396572 OTO - NOTNLM OT - MERS OT - MERS epidemiology OT - MERS-CoV OT - coronaviruses OT - severe acute respiratory infection (SARI) OT - zoonotic disease COIS- No competing financial interests exist. EDAT- 2019/01/25 06:00 MHDA- 2019/08/14 06:00 PMCR- 2019/02/26 CRDT- 2019/01/25 06:00 PHST- 2019/01/25 06:00 [pubmed] PHST- 2019/08/14 06:00 [medline] PHST- 2019/01/25 06:00 [entrez] PHST- 2019/02/26 00:00 [pmc-release] AID - 10.1089/vbz.2017.2191 [pii] AID - 10.1089/vbz.2017.2191 [doi] PST - ppublish SO - Vector Borne Zoonotic Dis. 2019 Mar;19(3):174-192. doi: 10.1089/vbz.2017.2191. Epub 2019 Jan 24. PMID- 28710271 OWN - NLM STAT- MEDLINE DCOM- 20180108 LR - 20221207 IS - 1098-5336 (Electronic) IS - 0099-2240 (Print) IS - 0099-2240 (Linking) VI - 83 IP - 18 DP - 2017 Sep 15 TI - Novel Alphacoronaviruses and Paramyxoviruses Cocirculate with Type 1 and Severe Acute Respiratory System (SARS)-Related Betacoronaviruses in Synanthropic Bats of Luxembourg. LID - 10.1128/AEM.01326-17 [doi] LID - e01326-17 AB - Several infectious disease outbreaks with high mortality in humans have been attributed to viruses that are thought to have evolved from bat viruses. In this study from Luxembourg, the genetic diversity and epidemiology of paramyxoviruses and coronaviruses shed by the bat species Rhinolophus ferrumequinum and Myotis emarginatus were evaluated. Feces collection (n = 624) was performed longitudinally in a mixed-species colony in 2015 and 2016. In addition, feces (n = 254) were collected cross-sectionally from six Myotis emarginatus colonies in 2016. By use of degenerate primers in a nested format, overall prevalences of 1.1% (10/878) and 4.9% (43/878) were determined for paramyxoviruses and coronaviruses. Sequences of the partial RNA-dependent RNA polymerase and spike glycoprotein genes of coronaviruses, as well as sequences of the partial L gene of paramyxoviruses, were obtained. Novel paramyxovirus and Alphacoronavirus strains were identified in different Myotis emarginatus colonies, and severe acute respiratory syndrome (SARS)-related Betacoronavirus strains were shed by Rhinolophus ferrumequinum Logistic regression revealed that the level of Alphacoronavirus shedding was highest in July (odds ratio, 2.8; P < 0.01), probably due to periparturient stress. Phylogenetic analyses point to close virus-host coevolution, and the high genetic similarity of the study strains suggests that the Myotis emarginatus colonies in Luxembourg are socially connected. Most interestingly, we show that bats also host Betacoronavirus1 strains. The high similarity of the spike gene sequences of these viruses with mammalian Betacoronavirus 1 strains may be of concern. Both the SARS-related and Betacoronavirus 1 strains detected in bats in Luxembourg may cross the species barrier after a host adaptation process.IMPORTANCE Bats are a natural reservoir of a number of zoonotic pathogens. Several severe outbreaks in humans (e.g., a Nipah virus outbreak in Malaysia in 1998, and the almost global spread of severe acute respiratory syndrome in 2003) have been caused by bat-borne viruses that were transmitted to humans mostly after virus adaptation (e.g., in intermediate animal hosts). Despite the indigenousness of bat species that host viruses with suspected zoonotic potential and despite the zoonotic transmission of European bat 1 lyssavirus in Luxembourg, knowledge about the diversity and epidemiology of bat viruses remains limited in this country. Moreover, in contrast to other European countries, bat viruses are currently not included in the national surveillance activities of this land-locked country. We suggest that this gap in disease surveillance should be addressed, since we show here that synanthropic bats host viruses that may be able to cross the species barrier. CI - Copyright © 2017 American Society for Microbiology. FAU - Pauly, Maude AU - Pauly M AD - Infectious Diseases Research Unit, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg maude.pauly@lih.lu. FAU - Pir, Jacques B AU - Pir JB AD - Section Zoologie des Vertébrés, Centre de Recherche Scientifique, Musée National d'Histoire Naturelle, Luxembourg, Luxembourg. FAU - Loesch, Catherine AU - Loesch C AD - Infectious Diseases Research Unit, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg. FAU - Sausy, Aurélie AU - Sausy A AD - Infectious Diseases Research Unit, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg. FAU - Snoeck, Chantal J AU - Snoeck CJ AD - Infectious Diseases Research Unit, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg. FAU - Hübschen, Judith M AU - Hübschen JM AD - Infectious Diseases Research Unit, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg. FAU - Muller, Claude P AU - Muller CP AD - Infectious Diseases Research Unit, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170831 PL - United States TA - Appl Environ Microbiol JT - Applied and environmental microbiology JID - 7605801 SB - IM MH - Alphacoronavirus/classification/genetics/*isolation & purification MH - Animals MH - Chiroptera/classification/*virology MH - Evolution, Molecular MH - Genetic Variation MH - Genome, Viral MH - Humans MH - Luxembourg MH - Paramyxovirinae/classification/genetics/*isolation & purification MH - Phylogeny MH - Severe acute respiratory syndrome-related coronavirus/classification/genetics/*isolation & purification PMC - PMC5583486 OTO - NOTNLM OT - Chiroptera OT - Luxembourg OT - coronavirus OT - molecular epidemiology OT - paramyxovirus OT - phylogenetic analysis OT - surveillance studies OT - virology OT - zoonotic infections EDAT- 2017/07/16 06:00 MHDA- 2018/01/09 06:00 PMCR- 2018/02/28 CRDT- 2017/07/16 06:00 PHST- 2017/06/14 00:00 [received] PHST- 2017/07/05 00:00 [accepted] PHST- 2017/07/16 06:00 [pubmed] PHST- 2018/01/09 06:00 [medline] PHST- 2017/07/16 06:00 [entrez] PHST- 2018/02/28 00:00 [pmc-release] AID - AEM.01326-17 [pii] AID - 01326-17 [pii] AID - 10.1128/AEM.01326-17 [doi] PST - epublish SO - Appl Environ Microbiol. 2017 Aug 31;83(18):e01326-17. doi: 10.1128/AEM.01326-17. Print 2017 Sep 15. PMID- 26038405 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20150603 LR - 20181113 IS - 2222-1751 (Print) IS - 2222-1751 (Electronic) IS - 2222-1751 (Linking) VI - 1 IP - 11 DP - 2012 Nov TI - Genetic relatedness of the novel human group C betacoronavirus to Tylonycteris bat coronavirus HKU4 and Pipistrellus bat coronavirus HKU5. PG - e35 LID - 10.1038/emi.2012.45 [doi] AB - The recent outbreak of severe respiratory infections associated with a novel group C betacoronavirus (HCoV-EMC) from Saudi Arabia has drawn global attention to another highly probable "SARS-like" animal-to-human interspecies jumping event in coronavirus (CoV). The genome of HCoV-EMC is most closely related to Tylonycteris bat coronavirus HKU4 (Ty-BatCoV HKU4) and Pipistrellus bat coronavirus HKU5 (Pi-BatCoV HKU5) we discovered in 2006. Phylogenetically, HCoV-EMC is clustered with Ty-BatCoV HKU4/Pi-BatCoV HKU5 with high bootstrap supports, indicating that HCoV-EMC is a group C betaCoV. The major difference between HCoV-EMC and Ty-BatCoV HKU4/Pi-BatCoV HKU5 is in the region between S and E, where HCoV-EMC possesses five ORFs (NS3a-NS3e) instead of four, with low (31%-62%) amino acid identities to Ty-BatCoV HKU4/Pi-BatCoV HKU5. Comparison of the seven conserved replicase domains for species demarcation shows that HCoV-EMC is a novel CoV species. More intensive surveillance studies in bats and other animals may reveal the natural host of HCoV-EMC. FAU - Woo, Patrick Cy AU - Woo PC AD - Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China. FAU - Lau, Susanna Kp AU - Lau SK AD - Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China. FAU - Li, Kenneth Sm AU - Li KS AD - Department of Microbiology, The University of Hong Kong , Hong Kong, China. FAU - Tsang, Alan Kl AU - Tsang AK AD - Department of Microbiology, The University of Hong Kong , Hong Kong, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - Department of Microbiology, The University of Hong Kong , Hong Kong, China ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong , Hong Kong, China ; Research Centre of Infection and Immunology, The University of Hong Kong , Hong Kong, China ; Carol Yu Centre for Infection, The University of Hong Kong , Hong Kong, China. LA - eng PT - Journal Article DEP - 20121107 PL - United States TA - Emerg Microbes Infect JT - Emerging microbes & infections JID - 101594885 PMC - PMC3630921 EDAT- 2012/11/01 00:00 MHDA- 2012/11/01 00:01 PMCR- 2012/11/01 CRDT- 2015/06/04 06:00 PHST- 2012/10/06 00:00 [received] PHST- 2012/10/09 00:00 [revised] PHST- 2012/10/09 00:00 [accepted] PHST- 2015/06/04 06:00 [entrez] PHST- 2012/11/01 00:00 [pubmed] PHST- 2012/11/01 00:01 [medline] PHST- 2012/11/01 00:00 [pmc-release] AID - emi201245 [pii] AID - 10.1038/emi.2012.45 [doi] PST - ppublish SO - Emerg Microbes Infect. 2012 Nov;1(11):e35. doi: 10.1038/emi.2012.45. Epub 2012 Nov 7. PMID- 29970183 OWN - NLM STAT- MEDLINE DCOM- 20181016 LR - 20181114 IS - 1297-9716 (Electronic) IS - 0928-4249 (Print) IS - 0928-4249 (Linking) VI - 49 IP - 1 DP - 2018 Jul 3 TI - Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells. PG - 55 LID - 10.1186/s13567-018-0551-9 [doi] LID - 55 AB - Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization. FAU - Szczepanski, Artur AU - Szczepanski A AD - Virogenetics, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. AD - Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Owczarek, Katarzyna AU - Owczarek K AD - Virogenetics, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. AD - Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Milewska, Aleksandra AU - Milewska A AD - Virogenetics, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. AD - Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Baster, Zbigniew AU - Baster Z AD - Institute of Physics, Faculty of Physics, Astronomy and Applied Computer Sciences, Jagiellonian University, Lojasiewicza 11, 30-348, Krakow, Poland. FAU - Rajfur, Zenon AU - Rajfur Z AD - Institute of Physics, Faculty of Physics, Astronomy and Applied Computer Sciences, Jagiellonian University, Lojasiewicza 11, 30-348, Krakow, Poland. FAU - Mitchell, Judy A AU - Mitchell JA AD - Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hatfield, Hertfordshire, AL9 7TA, UK. FAU - Pyrc, Krzysztof AU - Pyrc K AUID- ORCID: 0000-0002-3867-7688 AD - Virogenetics, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. k.a.pyrc@uj.edu.pl. AD - Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. k.a.pyrc@uj.edu.pl. LA - eng GR - UMO 2012/07/E/NZ6/01712/Narodowe Centrum Nauki (PL)/International GR - CM1407/European Cooperation in Science and Technology ()/International GR - UMO-2017/25/N/NZ6/01310/Narodowe Centrum Nauki/International PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180703 PL - England TA - Vet Res JT - Veterinary research JID - 9309551 RN - 0 (Caveolin 1) SB - IM MH - Caveolin 1/*metabolism MH - Cell Line, Tumor MH - Coronavirus Infections/*virology MH - Coronavirus, Canine/*physiology MH - Endocytosis MH - Humans MH - *Virus Internalization PMC - PMC6029178 EDAT- 2018/07/05 06:00 MHDA- 2018/10/17 06:00 PMCR- 2018/07/03 CRDT- 2018/07/05 06:00 PHST- 2018/02/14 00:00 [received] PHST- 2018/06/14 00:00 [accepted] PHST- 2018/07/05 06:00 [entrez] PHST- 2018/07/05 06:00 [pubmed] PHST- 2018/10/17 06:00 [medline] PHST- 2018/07/03 00:00 [pmc-release] AID - 10.1186/s13567-018-0551-9 [pii] AID - 551 [pii] AID - 10.1186/s13567-018-0551-9 [doi] PST - epublish SO - Vet Res. 2018 Jul 3;49(1):55. doi: 10.1186/s13567-018-0551-9. PMID- 25086180 OWN - NLM STAT- MEDLINE DCOM- 20150604 LR - 20211021 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 191 DP - 2014 Oct 13 TI - Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells. PG - 21-9 LID - S0168-1702(14)00300-1 [pii] LID - 10.1016/j.virusres.2014.07.022 [doi] AB - Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and inducing membrane fusion between viral envelopes and cellular membranes. However, the entry mechanism of PEDV is not studied. Here, we determined the entry mechanism of PEDV into Vero cells. Our data confirmed that PEDV entry followed clathrin-mediated endocytosis independence of caveolae-coated pit assembly. The internalized PEDV was co-localized with the clathrin-mediated endocytic marker, but not with the caveolae-mediated endocytic marker. In addition, cells treated with lysosomotropic agents and serine protease inhibitors were resistant to PEDV. Our data revealed that PEDV entry followed clathrin-mediated endocytosis and was dependent on a low pH and serine proteolysis for successful entry into cells. CI - Copyright © 2014 Elsevier B.V. All rights reserved. FAU - Park, Jung-Eun AU - Park JE AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, South Korea. FAU - Cruz, Deu John M AU - Cruz DJ AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, South Korea. FAU - Shin, Hyun-Jin AU - Shin HJ AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, South Korea; Research Institute of Veterinary Medicine, Chungnam National University, South Korea. Electronic address: shin0089@cnu.ac.kr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140731 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Clathrin) RN - EC 3.4.21.- (Serine Endopeptidases) SB - IM MH - Animals MH - Chlorocebus aethiops MH - Clathrin/*metabolism MH - Coronavirus Infections/enzymology/metabolism/*veterinary/virology MH - Endocytosis MH - Porcine epidemic diarrhea virus/*physiology MH - Serine Endopeptidases/*metabolism MH - Swine MH - Swine Diseases/*enzymology/metabolism/virology MH - Vero Cells MH - *Virus Internalization PMC - PMC7114442 OTO - NOTNLM OT - Clathrin OT - Coronavirus OT - Entry OT - Low pH OT - Porcine epidemic diarrhea OT - Serine protease EDAT- 2014/08/03 06:00 MHDA- 2015/06/05 06:00 PMCR- 2014/07/31 CRDT- 2014/08/03 06:00 PHST- 2014/06/22 00:00 [received] PHST- 2014/07/17 00:00 [revised] PHST- 2014/07/21 00:00 [accepted] PHST- 2014/08/03 06:00 [entrez] PHST- 2014/08/03 06:00 [pubmed] PHST- 2015/06/05 06:00 [medline] PHST- 2014/07/31 00:00 [pmc-release] AID - S0168-1702(14)00300-1 [pii] AID - 10.1016/j.virusres.2014.07.022 [doi] PST - ppublish SO - Virus Res. 2014 Oct 13;191:21-9. doi: 10.1016/j.virusres.2014.07.022. Epub 2014 Jul 31. PMID- 27639955 OWN - NLM STAT- MEDLINE DCOM- 20170731 LR - 20210119 IS - 1879-0984 (Electronic) IS - 0166-0934 (Print) IS - 0166-0934 (Linking) VI - 237 DP - 2016 Nov TI - Generation and Characterization of Eptesicus fuscus (Big brown bat) kidney cell lines immortalized using the Myotis polyomavirus large T-antigen. PG - 166-173 LID - S0166-0934(16)30244-0 [pii] LID - 10.1016/j.jviromet.2016.09.008 [doi] AB - It is speculated that bats are important reservoir hosts for numerous viruses, with 27 viral families reportedly detected in bats. Majority of these viruses have not been isolated and there is little information regarding their biology in bats. Establishing a well-characterized bat cell line supporting the replication of bat-borne viruses would facilitate the analysis of virus-host interactions in an in vitro model. Currently, few bat cell lines have been developed and only Tb1-Lu, derived from Tadarida brasiliensis is commercially available. Here we describe a method to establish and immortalize big brown bat (Eptesicus fuscus) kidney (Efk3) cells using the Myotis polyomavirus T-antigen. Subclones of this cell line expressed both epithelial and fibroblast markers to varying extents. Cell clones expressed interferon beta in response to poly(I:C) stimulation and supported the replication of four different viruses, namely, vesicular stomatitis virus (VSV), porcine epidemic diarrhea coronavirus (PED-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and herpes simplex virus (HSV). To our knowledge, this is the first bat cell line from a northern latitude insectivorous bat developed using a novel technology. The cell line has the potential to be used for isolation of bat viruses and for studying virus-bat interactions in culture. CI - Copyright © 2016 Elsevier B.V. All rights reserved. FAU - Banerjee, Arinjay AU - Banerjee A AD - Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Rapin, Noreen AU - Rapin N AD - Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Miller, Megan AU - Miller M AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA. FAU - Griebel, Philip AU - Griebel P AD - Vaccine and Infectious Disease Organization - International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Zhou, Yan AU - Zhou Y AD - Vaccine and Infectious Disease Organization - International Vaccine Center (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Munster, Vincent AU - Munster V AD - Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT, USA. FAU - Misra, Vikram AU - Misra V AD - Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. Electronic address: vikram.misra@usask.ca. LA - eng PT - Journal Article PT - Research Support, N.I.H., Intramural PT - Research Support, Non-U.S. Gov't DEP - 20160914 PL - Netherlands TA - J Virol Methods JT - Journal of virological methods JID - 8005839 RN - 0 (Antigens, Polyomavirus Transforming) RN - 0 (Vimentin) RN - 68238-35-7 (Keratins) SB - IM MH - Animals MH - *Antigens, Polyomavirus Transforming MH - Cell Culture Techniques MH - *Cell Line MH - *Cell Transformation, Viral MH - *Chiroptera MH - Epithelial Cells/virology MH - Fibroblasts/virology MH - Keratins/genetics MH - *Kidney MH - Middle East Respiratory Syndrome Coronavirus/growth & development MH - Polyomavirus/growth & development/*physiology MH - Porcine epidemic diarrhea virus/growth & development MH - Simplexvirus/growth & development MH - Vesiculovirus/growth & development MH - Vimentin/genetics PMC - PMC7113758 OTO - NOTNLM OT - Big brown bat OT - Cell-line OT - HSV OT - Kidney OT - MERS-CoV OT - PED-CoV OT - VSV EDAT- 2016/09/19 06:00 MHDA- 2017/08/02 06:00 PMCR- 2016/09/14 CRDT- 2016/09/19 06:00 PHST- 2016/05/11 00:00 [received] PHST- 2016/09/06 00:00 [revised] PHST- 2016/09/07 00:00 [accepted] PHST- 2016/09/19 06:00 [pubmed] PHST- 2017/08/02 06:00 [medline] PHST- 2016/09/19 06:00 [entrez] PHST- 2016/09/14 00:00 [pmc-release] AID - S0166-0934(16)30244-0 [pii] AID - 10.1016/j.jviromet.2016.09.008 [doi] PST - ppublish SO - J Virol Methods. 2016 Nov;237:166-173. doi: 10.1016/j.jviromet.2016.09.008. Epub 2016 Sep 14. PMID- 25888853 OWN - NLM STAT- MEDLINE DCOM- 20160324 LR - 20181113 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 12 DP - 2015 Mar 12 TI - Detection of new genetic variants of Betacoronaviruses in Endemic Frugivorous Bats of Madagascar. PG - 42 LID - 10.1186/s12985-015-0271-y [doi] LID - 42 AB - BACKGROUND: Bats are amongst the natural reservoirs of many coronaviruses (CoVs) of which some can lead to severe infection in human. African bats are known to harbor a range of pathogens (e.g., Ebola and Marburg viruses) that can infect humans and cause disease outbreaks. A recent study in South Africa isolated a genetic variant closely related to MERS-CoV from an insectivorous bat. Though Madagascar is home to 44 bat species (41 insectivorous and 3 frugivorous) of which 34 are endemic, no data exists concerning the circulation of CoVs in the island's chiropteran fauna. Certain Malagasy bats can be frequently found in close contact with humans and frugivorous bats feed in the same trees where people collect and consume fruits and are hunted and consumed as bush meat. The purpose of our study is to detect and identify CoVs from frugivorous bats in Madagascar to evaluate the risk of human infection from infected bats. METHODS: Frugivorous bats belonging to three species were captured in four different regions of Madagascar. We analyzed fecal and throat swabs to detect the presence of virus through amplification of the RNA-dependent RNA polymerase (RdRp) gene, which is highly conserved in all known coronaviruses. Phylogenetic analyses were performed from positive specimens. RESULTS: From 351 frugivorous bats, we detected 14 coronaviruses from two endemic bats species, of which 13 viruses were identified from Pteropus rufus and one from Eidolon dupreanum, giving an overall prevalence of 4.5%. Phylogenetic analysis revealed that the Malagasy strains belong to the genus Betacoronavirus but form three distinct clusters, which seem to represent previously undescribed genetic lineages. CONCLUSIONS: Our findings suggest that CoVs circulate in frugivorous bats of Madagascar, demonstrating the needs to evaluate spillover risk to human populations especially for individuals that hunt and consume infected bats. Possible dispersal mechanisms as to how coronaviruses arrived on Madagascar are discussed. FAU - Razanajatovo, Norosoa H AU - Razanajatovo NH AD - Virology Unit, Institut Pasteur of Madagascar, Ambatofotsikely, BP 1274, Antananarivo, Madagascar, Dummy_Only. norosoa@pasteur.mg. FAU - Nomenjanahary, Lalaina A AU - Nomenjanahary LA AD - Virology Unit, Institut Pasteur of Madagascar, Ambatofotsikely, BP 1274, Antananarivo, Madagascar, Dummy_Only. lalaina@pasteur.mg. FAU - Wilkinson, David A AU - Wilkinson DA AD - Centre de Recherche et de Veille sur les Maladies Emergentes dans l'Ocean Indien (CRVOI), Plateforme de Recherche CYROI, 2 rue Maxime Riviere, 97490, Sainte Clotilde, La Reunion, France. dwilkin799@gmail.com. FAU - Razafimanahaka, Julie H AU - Razafimanahaka JH AD - Madagasikara Voakajy, BP 5181, Antananarivo, Madagascar. hantajulie@gmail.com. AD - Department of Animal Biology, Faculty of Sciences, University of Antananarivo, BP 906, Antananarivo, Madagascar. hantajulie@gmail.com. FAU - Goodman, Steven M AU - Goodman SM AD - Association Vahatra, BP 3972, Antananarivo, Madagascar. sgoodman@fieldmuseum.org. FAU - Jenkins, Richard K AU - Jenkins RK AD - School of Environment, Natural Resources and Geography, Bangor University, Bangor, Gwynedd, United Kingdom. rkbjenkins@gmail.com. FAU - Jones, Julia P G AU - Jones JP AD - School of Environment, Natural Resources and Geography, Bangor University, Bangor, Gwynedd, United Kingdom. julia.jones@bangor.ac.uk. FAU - Heraud, Jean-Michel AU - Heraud JM AD - Virology Unit, Institut Pasteur of Madagascar, Ambatofotsikely, BP 1274, Antananarivo, Madagascar, Dummy_Only. jmheraud@pasteur.mg. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150312 PL - England TA - Virol J JT - Virology journal JID - 101231645 SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronaviridae/classification/*genetics/isolation & purification MH - Coronaviridae Infections/*veterinary MH - Genes, Viral MH - *Genetic Variation MH - Geography MH - Madagascar MH - Phylogeny PMC - PMC4404003 EDAT- 2015/04/19 06:00 MHDA- 2016/03/25 06:00 PMCR- 2015/03/12 CRDT- 2015/04/19 06:00 PHST- 2014/09/04 00:00 [received] PHST- 2015/02/24 00:00 [accepted] PHST- 2015/04/19 06:00 [entrez] PHST- 2015/04/19 06:00 [pubmed] PHST- 2016/03/25 06:00 [medline] PHST- 2015/03/12 00:00 [pmc-release] AID - 10.1186/s12985-015-0271-y [pii] AID - 271 [pii] AID - 10.1186/s12985-015-0271-y [doi] PST - epublish SO - Virol J. 2015 Mar 12;12:42. doi: 10.1186/s12985-015-0271-y. PMID- 25187545 OWN - NLM STAT- MEDLINE DCOM- 20150210 LR - 20220223 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 88 IP - 22 DP - 2014 Nov TI - Human coronavirus NL63 utilizes heparan sulfate proteoglycans for attachment to target cells. PG - 13221-30 LID - 10.1128/JVI.02078-14 [doi] AB - Human coronavirus NL63 (HCoV-NL63) is an alphacoronavirus that was first identified in 2004 in the nasopharyngeal aspirate from a 7-month-old patient with a respiratory tract infection. Previous studies showed that HCoV-NL63 and the genetically distant severe acute respiratory syndrome (SARS)-CoV employ the same receptor for host cell entry, angiotensin-converting enzyme 2 (ACE2), but it is largely unclear whether ACE2 interactions are sufficient to allow HCoV-NL63 binding to cells. The present study showed that directed expression of angiotensin-converting enzyme 2 (ACE2) on cells previously resistant to HCoV-NL63 renders them susceptible, showing that ACE2 protein acts as a functional receptor and that its expression is required for infection. However, comparative analysis showed that directed expression or selective scission of the ACE2 protein had no measurable effect on virus adhesion. In contrast, binding of HCoV-NL63 to heparan sulfates was required for viral attachment and infection of target cells, showing that these molecules serve as attachment receptors for HCoV-NL63. IMPORTANCE: ACE2 protein was proposed as a receptor for HCoV-NL63 already in 2005, but an in-depth analysis of early events during virus infection had not been performed thus far. Here, we show that the ACE2 protein is required for viral entry but that it is not the primary binding site on the cell surface. Conducted research showed that heparan sulfate proteoglycans function as adhesion molecules, increasing the virus density on cell surface and possibly facilitating the interaction between HCoV-NL63 and its receptor. Obtained results show that the initial events during HCoV-NL63 infection are more complex than anticipated and that a newly described interaction may be essential for understanding the infection process and, possibly, also assist in drug design. CI - Copyright © 2014, American Society for Microbiology. All Rights Reserved. FAU - Milewska, Aleksandra AU - Milewska A AD - Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Zarebski, Miroslaw AU - Zarebski M AD - Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Nowak, Paulina AU - Nowak P AD - Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Stozek, Karol AU - Stozek K AD - Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. FAU - Potempa, Jan AU - Potempa J AD - Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland Department of Oral Immunology and Infectious Diseases, School of Dentistry, University of Louisville, Louisville, Kentucky, USA. FAU - Pyrc, Krzysztof AU - Pyrc K AD - Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland k.a.pyrc@uj.edu.pl. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140903 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Heparan Sulfate Proteoglycans) RN - EC 3.4.15.1 (Peptidyl-Dipeptidase A) RN - EC 3.4.17.23 (ACE2 protein, human) RN - EC 3.4.17.23 (Angiotensin-Converting Enzyme 2) SB - IM MH - Angiotensin-Converting Enzyme 2 MH - Animals MH - Cell Line MH - Coronavirus NL63, Human/*physiology MH - Heparan Sulfate Proteoglycans/*metabolism MH - Humans MH - Peptidyl-Dipeptidase A/genetics/*metabolism MH - *Virus Attachment PMC - PMC4249106 EDAT- 2014/09/05 06:00 MHDA- 2015/02/11 06:00 PMCR- 2015/05/01 CRDT- 2014/09/05 06:00 PHST- 2014/09/05 06:00 [entrez] PHST- 2014/09/05 06:00 [pubmed] PHST- 2015/02/11 06:00 [medline] PHST- 2015/05/01 00:00 [pmc-release] AID - JVI.02078-14 [pii] AID - 02078-14 [pii] AID - 10.1128/JVI.02078-14 [doi] PST - ppublish SO - J Virol. 2014 Nov;88(22):13221-30. doi: 10.1128/JVI.02078-14. Epub 2014 Sep 3. PMID- 29500692 OWN - NLM STAT- MEDLINE DCOM- 20181001 LR - 20190302 IS - 1995-820X (Electronic) IS - 1674-0769 (Print) IS - 1995-820X (Linking) VI - 33 IP - 1 DP - 2018 Feb TI - Longitudinal Surveillance of Betacoronaviruses in Fruit Bats in Yunnan Province, China During 2009-2016. PG - 87-95 LID - 10.1007/s12250-018-0017-2 [doi] AB - Previous studies indicated that fruit bats carry two betacoronaviruses, BatCoV HKU9 and BatCoV GCCDC1. To investigate the epidemiology and genetic diversity of these coronaviruses, we conducted a longitudinal surveillance in fruit bats in Yunnan province, China during 2009-2016. A total of 59 (10.63%) bat samples were positive for the two betacorona-viruses, 46 (8.29%) for HKU9 and 13 (2.34%) for GCCDC1, or closely related viruses. We identified a novel HKU9 strain, tentatively designated as BatCoV HKU9-2202, by sequencing the full-length genome. The BatCoV HKU9-2202 shared 83% nucleotide identity with other BatCoV HKU9 stains based on whole genome sequences. The most divergent region is in the spike protein, which only shares 68% amino acid identity with BatCoV HKU9. Quantitative PCR revealed that the intestine was the primary infection organ of BatCoV HKU9 and GCCDC1, but some HKU9 was also detected in the heart, kidney, and lung tissues of bats. This study highlights the importance of virus surveillance in natural reservoirs and emphasizes the need for preparedness against the potential spill-over of these viruses to local residents living near bat caves. FAU - Luo, Yun AU - Luo Y AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Li, Bei AU - Li B AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Jiang, Ren-Di AU - Jiang RD AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Hu, Bing-Jie AU - Hu BJ AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Luo, Dong-Sheng AU - Luo DS AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Zhu, Guang-Jian AU - Zhu GJ AD - EcoHealth Alliance, New York, NY, 10001, USA. FAU - Hu, Ben AU - Hu B AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Liu, Hai-Zhou AU - Liu HZ AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Zhang, Yun-Zhi AU - Zhang YZ AD - Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China. AD - School of Public Health, Dali University, Dali, 671000, China. FAU - Yang, Xing-Lou AU - Yang XL AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Shi, Zheng-Li AU - Shi ZL AUID- ORCID: 0000-0001-8089-163X AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn. LA - eng GR - R01 AI110964/AI/NIAID NIH HHS/United States PT - Journal Article DEP - 20180302 PL - Netherlands TA - Virol Sin JT - Virologica Sinica JID - 101514185 RN - 0 (RNA, Viral) SB - IM MH - Animal Structures/virology MH - Animals MH - Betacoronavirus/classification/genetics/*isolation & purification MH - China/epidemiology MH - Chiroptera/*virology MH - Coronavirus Infections/*veterinary/virology MH - Disease Reservoirs MH - Epidemiological Monitoring MH - *Genetic Variation MH - RNA, Viral/genetics MH - Real-Time Polymerase Chain Reaction MH - Sequence Analysis, DNA MH - Whole Genome Sequencing PMC - PMC6178081 OTO - NOTNLM OT - Betacoronavirus OT - Genetic diversity OT - Surveillance COIS- CONFLICT OF INTEREST: The authors declare that they have no conflict of interest. ANIMAL AND HUMAN RIGHTS STATEMENT: This study was approved by the Animal Ethics Committee of the Wuhan Institute of Virology. All institutional and national guidelines for the care and use of animals were followed. EDAT- 2018/03/04 06:00 MHDA- 2018/10/03 06:00 PMCR- 2019/03/02 CRDT- 2018/03/04 06:00 PHST- 2017/12/26 00:00 [received] PHST- 2018/01/30 00:00 [accepted] PHST- 2018/03/04 06:00 [pubmed] PHST- 2018/10/03 06:00 [medline] PHST- 2018/03/04 06:00 [entrez] PHST- 2019/03/02 00:00 [pmc-release] AID - 10.1007/s12250-018-0017-2 [pii] AID - 17 [pii] AID - 10.1007/s12250-018-0017-2 [doi] PST - ppublish SO - Virol Sin. 2018 Feb;33(1):87-95. doi: 10.1007/s12250-018-0017-2. Epub 2018 Mar 2. PMID- 30208582 OWN - NLM STAT- MEDLINE DCOM- 20181112 LR - 20181114 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 10 IP - 9 DP - 2018 Sep 11 TI - Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark. LID - 10.3390/v10090486 [doi] LID - 486 AB - Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations. FAU - Lazov, Christina M AU - Lazov CM AD - DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. chmari@vet.dtu.dk. FAU - Chriél, Mariann AU - Chriél M AD - DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. march@vet.dtu.dk. FAU - Baagøe, Hans J AU - Baagøe HJ AD - The Natural History Museum of Denmark, University of Copenhagen, 2100 Copenhagen, Denmark. hjbaagoe@snm.ku.dk. FAU - Fjederholt, Esben AU - Fjederholt E AD - The Natural History Museum of Denmark, University of Copenhagen, 2100 Copenhagen, Denmark. esbenfjederholt@gmail.com. FAU - Deng, Yu AU - Deng Y AD - DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. dengyu127@163.com. AD - School of Animal Science, Xichang College, Xichang 615013, China. dengyu127@163.com. FAU - Kooi, Engbert A AU - Kooi EA AD - Wageningen Bioveterinary Research, 8221 RA Lelystad, The Netherlands. bart.kooi@wur.nl. FAU - Belsham, Graham J AU - Belsham GJ AUID- ORCID: 0000-0003-1187-4873 AD - DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. grbe@vet.dtu.dk. FAU - Bøtner, Anette AU - Bøtner A AD - DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. aneb@vet.dtu.dk. FAU - Rasmussen, Thomas Bruun AU - Rasmussen TB AUID- ORCID: 0000-0002-4241-1559 AD - DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark. tbrur@vet.dtu.dk. LA - eng GR - None/Danish Veterinary and Food Administration/International GR - CSC201408515154/China Scholarship Council/International PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180911 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Alphacoronavirus/*classification/genetics/*isolation & purification MH - Animals MH - Chiroptera/*virology MH - Cluster Analysis MH - Coronavirus Infections/*veterinary/virology MH - Denmark MH - Feces/virology MH - *Genetic Variation MH - Phylogeny MH - Real-Time Polymerase Chain Reaction MH - Reverse Transcriptase Polymerase Chain Reaction MH - Sequence Analysis, DNA MH - Sequence Homology PMC - PMC6163574 OTO - NOTNLM OT - Europe OT - Vespertilionidae OT - coronavirus OT - host restriction OT - nucleotide sequencing OT - phylogenetic analysis COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. EDAT- 2018/09/14 06:00 MHDA- 2018/11/13 06:00 PMCR- 2018/09/01 CRDT- 2018/09/14 06:00 PHST- 2018/08/03 00:00 [received] PHST- 2018/09/03 00:00 [revised] PHST- 2018/09/10 00:00 [accepted] PHST- 2018/09/14 06:00 [entrez] PHST- 2018/09/14 06:00 [pubmed] PHST- 2018/11/13 06:00 [medline] PHST- 2018/09/01 00:00 [pmc-release] AID - v10090486 [pii] AID - viruses-10-00486 [pii] AID - 10.3390/v10090486 [doi] PST - epublish SO - Viruses. 2018 Sep 11;10(9):486. doi: 10.3390/v10090486. PMID- 28840816 OWN - NLM STAT- MEDLINE DCOM- 20170919 LR - 20200320 IS - 1465-2099 (Electronic) IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 98 IP - 9 DP - 2017 Sep TI - A persistently infecting coronavirus in hibernating Myotis lucifugus, the North American little brown bat. PG - 2297-2309 LID - 10.1099/jgv.0.000898 [doi] AB - Bats are important reservoir hosts for emerging viruses, including coronaviruses that cause diseases in people. Although there have been several studies on the pathogenesis of coronaviruses in humans and surrogate animals, there is little information on the interactions of these viruses with their natural bat hosts. We detected a coronavirus in the intestines of 53/174 hibernating little brown bats (Myotis lucifugus), as well as in the lungs of some of these individuals. Interestingly, the presence of the virus was not accompanied by overt inflammation. Viral RNA amplified from little brown bats in this study appeared to be from two distinct clades. The sequences in clade 1 were very similar to the archived sequence derived from little brown bats and the sequences from clade 2 were more closely related to the archived sequence from big brown bats. This suggests that two closely related coronaviruses may circulate in little brown bats. Sequence variation among coronavirus detected from individual bats suggested that infection occurred prior to hibernation, and that the virus persisted for up to 4 months of hibernation in the laboratory. Based on the sequence of its genome, the coronavirus was placed in the Alphacoronavirus genus, along with some human coronaviruses, bat viruses and the porcine epidemic diarrhoea virus. The detection and identification of an apparently persistent coronavirus in a local bat species creates opportunities to understand the dynamics of coronavirus circulation in bat populations. FAU - Subudhi, Sonu AU - Subudhi S AD - Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Rapin, Noreen AU - Rapin N AD - Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Bollinger, Trent K AU - Bollinger TK AD - Department of Pathology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Hill, Janet E AU - Hill JE AD - Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Donaldson, Michael E AU - Donaldson ME AD - Trent University, Peterborough, Ontario, Canada. FAU - Davy, Christina M AU - Davy CM AD - Trent University, Peterborough, Ontario, Canada. FAU - Warnecke, Lisa AU - Warnecke L AD - Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada. FAU - Turner, James M AU - Turner JM AD - Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada. FAU - Kyle, Christopher J AU - Kyle CJ AD - Trent University, Peterborough, Ontario, Canada. FAU - Willis, Craig K R AU - Willis CKR AD - Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada. FAU - Misra, Vikram AU - Misra V AD - Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. LA - eng PT - Journal Article DEP - 20170825 PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronavirus/genetics/*isolation & purification/physiology MH - Coronavirus Infections/pathology/*veterinary/virology MH - Lung/pathology/virology MH - Phylogeny MH - United States PMC - PMC7079692 EDAT- 2017/08/26 06:00 MHDA- 2017/09/20 06:00 PMCR- 2017/08/25 CRDT- 2017/08/26 06:00 PHST- 2017/08/26 06:00 [pubmed] PHST- 2017/09/20 06:00 [medline] PHST- 2017/08/26 06:00 [entrez] PHST- 2017/08/25 00:00 [pmc-release] AID - 000898 [pii] AID - 10.1099/jgv.0.000898 [doi] PST - ppublish SO - J Gen Virol. 2017 Sep;98(9):2297-2309. doi: 10.1099/jgv.0.000898. Epub 2017 Aug 25. PMID- 28956766 OWN - NLM STAT- MEDLINE DCOM- 20171206 LR - 20181113 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 91 IP - 23 DP - 2017 Dec 1 TI - Porcine Hemagglutinating Encephalomyelitis Virus Enters Neuro-2a Cells via Clathrin-Mediated Endocytosis in a Rab5-, Cholesterol-, and pH-Dependent Manner. LID - 10.1128/JVI.01083-17 [doi] LID - e01083-17 AB - Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that invades the central nervous system (CNS) in piglets. Although important progress has been made toward understanding the biology of PHEV, many aspects of its life cycle remain obscure. Here we dissected the molecular mechanism underlying cellular entry and intracellular trafficking of PHEV in mouse neuroblastoma (Neuro-2a) cells. We first performed a thin-section transmission electron microscopy (TEM) assay to characterize the kinetics of PHEV, and we found that viral entry and transfer occur via membranous coating-mediated endo- and exocytosis. To verify the roles of distinct endocytic pathways, systematic approaches were used, including pharmacological inhibition, RNA interference, confocal microscopy analysis, use of fluorescently labeled virus particles, and overexpression of a dominant negative (DN) mutant. Quantification of infected cells showed that PHEV enters cells by clathrin-mediated endocytosis (CME) and that low pH, dynamin, cholesterol, and Eps15 are indispensably involved in this process. Intriguingly, PHEV invasion leads to rapid actin rearrangement, suggesting that the intactness and dynamics of the actin cytoskeleton are positively correlated with viral endocytosis. We next investigated the trafficking of internalized PHEV and found that Rab5- and Rab7-dependent pathways are required for the initiation of a productive infection. Furthermore, a GTPase activation assay suggested that endogenous Rab5 is activated by PHEV and is crucial for viral progression. Our findings demonstrate that PHEV hijacks the CME and endosomal system of the host to enter and traffic within neural cells, providing new insights into PHEV pathogenesis and guidance for antiviral drug design.IMPORTANCE Porcine hemagglutinating encephalomyelitis virus (PHEV), a nonsegmented, positive-sense, single-stranded RNA coronavirus, invades the central nervous system (CNS) and causes neurological dysfunction. Neural cells are its targets for viral progression. However, the detailed mechanism underlying PHEV entry and trafficking remains unknown. PHEV is the etiological agent of porcine hemagglutinating encephalomyelitis, which is an acute and highly contagious disease that causes numerous deaths in suckling piglets and enormous economic losses in China. Understanding the viral entry pathway will not only advance our knowledge of PHEV infection and pathogenesis but also open new approaches to the development of novel therapeutic strategies. Therefore, we employed systematic approaches to dissect the internalization and intracellular trafficking mechanism of PHEV in Neuro-2a cells. This is the first report to describe the process of PHEV entry into nerve cells via clathrin-mediated endocytosis in a dynamin-, cholesterol-, and pH-dependent manner that requires Rab5 and Rab7. CI - Copyright © 2017 Li et al. FAU - Li, Zi AU - Li Z AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Zhao, Kui AU - Zhao K AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lan, Yungang AU - Lan Y AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lv, Xiaoling AU - Lv X AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Hu, Shiyu AU - Hu S AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Guan, Jiyu AU - Guan J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lu, Huijun AU - Lu H AD - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China. FAU - Zhang, Jing AU - Zhang J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Shi, Junchao AU - Shi J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Yang, Yawen AU - Yang Y AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Song, Deguang AU - Song D AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Gao, Feng AU - Gao F AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - He, Wenqi AU - He W AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China hewq@jlu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20171114 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Actins) RN - 0 (Adaptor Proteins, Signal Transducing) RN - 0 (Clathrin) RN - 0 (Eps15 protein, mouse) RN - 97C5T2UQ7J (Cholesterol) RN - EC 3.6.5.2 (rab5 GTP-Binding Proteins) RN - EC 3.6.5.5 (Dynamins) SB - IM MH - Actin Cytoskeleton/metabolism MH - Actins/metabolism MH - Adaptor Proteins, Signal Transducing/metabolism MH - Animals MH - Betacoronavirus 1/drug effects/genetics/pathogenicity/*physiology MH - Cell Line, Tumor MH - Cholesterol/*metabolism MH - Clathrin/*metabolism MH - Dynamins/metabolism MH - *Endocytosis MH - Hydrogen-Ion Concentration MH - Kinetics MH - Mice MH - Mutation MH - Neuroblastoma MH - RNA Interference MH - *Virus Internalization MH - rab5 GTP-Binding Proteins/*metabolism PMC - PMC5686734 OTO - NOTNLM OT - Rab OT - clathrin OT - endocytosis OT - neurovirulent coronavirus OT - porcine hemagglutinating encephalomyelitis virus EDAT- 2017/09/29 06:00 MHDA- 2017/12/07 06:00 PMCR- 2017/11/14 CRDT- 2017/09/29 06:00 PHST- 2017/06/29 00:00 [received] PHST- 2017/09/07 00:00 [accepted] PHST- 2017/09/29 06:00 [pubmed] PHST- 2017/12/07 06:00 [medline] PHST- 2017/09/29 06:00 [entrez] PHST- 2017/11/14 00:00 [pmc-release] AID - JVI.01083-17 [pii] AID - 01083-17 [pii] AID - 10.1128/JVI.01083-17 [doi] PST - epublish SO - J Virol. 2017 Nov 14;91(23):e01083-17. doi: 10.1128/JVI.01083-17. Print 2017 Dec 1. PMID- 30209269 OWN - NLM STAT- MEDLINE DCOM- 20190122 LR - 20201228 IS - 2222-1751 (Electronic) IS - 2222-1751 (Linking) VI - 7 IP - 1 DP - 2018 Sep 12 TI - Genomic characterization and infectivity of a novel SARS-like coronavirus in Chinese bats. PG - 154 LID - 10.1038/s41426-018-0155-5 [doi] LID - 154 AB - SARS coronavirus (SARS-CoV), the causative agent of the large SARS outbreak in 2003, originated in bats. Many SARS-like coronaviruses (SL-CoVs) have been detected in bats, particularly those that reside in China, Europe, and Africa. To further understand the evolutionary relationship between SARS-CoV and its reservoirs, 334 bats were collected from Zhoushan city, Zhejiang province, China, between 2015 and 2017. PCR amplification of the conserved coronaviral protein RdRp detected coronaviruses in 26.65% of bats belonging to this region, and this number was influenced by seasonal changes. Full genomic analyses of the two new SL-CoVs from Zhoushan (ZXC21 and ZC45) showed that their genomes were 29,732 nucleotides (nt) and 29,802 nt in length, respectively, with 13 open reading frames (ORFs). These results revealed 81% shared nucleotide identity with human/civet SARS CoVs, which was more distant than that observed previously for bat SL-CoVs in China. Importantly, using pathogenic tests, we found that the virus can reproduce and cause disease in suckling rats, and further studies showed that the virus-like particles can be observed in the brains of suckling rats by electron microscopy. Thus, this study increased our understanding of the genetic diversity of the SL-CoVs carried by bats and also provided a new perspective to study the possibility of cross-species transmission of SL-CoVs using suckling rats as an animal model. FAU - Hu, Dan AU - Hu D AD - Department of Epidemiology, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China. AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Zhu, Changqiang AU - Zhu C AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Ai, Lele AU - Ai L AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - He, Ting AU - He T AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Wang, Yi AU - Wang Y AD - Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province, 214064, P.R. China. FAU - Ye, Fuqiang AU - Ye F AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Yang, Lu AU - Yang L AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Ding, Chenxi AU - Ding C AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Zhu, Xuhui AU - Zhu X AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Lv, Ruicheng AU - Lv R AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Zhu, Jin AU - Zhu J AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Hassan, Bachar AU - Hassan B AD - Stony Brook University, Stony Brook, 11794, USA. FAU - Feng, Youjun AU - Feng Y AD - Department of Pathogen Biology & Microbiology and Department of General Intensive Care Unit of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, China. fengyj@zju.edu.cn. FAU - Tan, Weilong AU - Tan W AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. njcdc@163.com. FAU - Wang, Changjun AU - Wang C AD - Department of Epidemiology, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China. science2008@hotmail.com. AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. science2008@hotmail.com. LA - eng GR - U1602223/National Natural Science Foundation of China (National Science Foundation of China)/ GR - BE2017620/Science and Technology Support Program of Jiangsu Province (Jiangsu Province Science and Technology Support Program)/ PT - Journal Article DEP - 20180912 PL - United States TA - Emerg Microbes Infect JT - Emerging microbes & infections JID - 101594885 RN - 0 (Viral Proteins) SB - IM EIN - Emerg Microbes Infect. 2020 Dec;9(1):2727. doi: 10.1080/22221751.2020.1857048. PMID: 33356975 MH - Animals MH - China MH - Chiroptera/*virology MH - Coronavirus/classification/*genetics/isolation & purification/*pathogenicity MH - Female MH - *Genome, Viral MH - Male MH - Open Reading Frames MH - Phylogeny MH - Rats MH - Viral Proteins/chemistry/genetics/metabolism MH - Virulence PMC - PMC6135831 COIS- All authors declare that they have no conflicts of interest. EDAT- 2018/09/14 06:00 MHDA- 2019/01/23 06:00 PMCR- 2018/09/12 CRDT- 2018/09/14 06:00 PHST- 2018/04/10 00:00 [received] PHST- 2018/08/02 00:00 [accepted] PHST- 2018/07/09 00:00 [revised] PHST- 2018/09/14 06:00 [entrez] PHST- 2018/09/14 06:00 [pubmed] PHST- 2019/01/23 06:00 [medline] PHST- 2018/09/12 00:00 [pmc-release] AID - 10.1038/s41426-018-0155-5 [pii] AID - 155 [pii] AID - 10.1038/s41426-018-0155-5 [doi] PST - epublish SO - Emerg Microbes Infect. 2018 Sep 12;7(1):154. doi: 10.1038/s41426-018-0155-5. PMID- 26319601 OWN - NLM STAT- MEDLINE DCOM- 20160512 LR - 20190522 IS - 1943-4936 (Electronic) IS - 1040-6387 (Linking) VI - 27 IP - 5 DP - 2015 Sep TI - Glomerulonephritis in a ferret with feline coronavirus infection. PG - 637-40 LID - 10.1177/1040638715599570 [doi] AB - A male domestic ferret (Mustela putorius furo), which was purchased from outside of Japan at 13 weeks of age, was euthanized at 18 months of age because of poor health. At autopsy, the liver, spleen, and mesenteric lymph node were enlarged, and white foci were observed on the outer surface of the liver. The outer surface of the mesenteric lymph node was dark red. Histologically, granulomas were observed in the liver, spleen, bone marrow, and lymph nodes, composed mainly of aggregated epithelioid macrophages, some of which were positive to an anti-feline coronavirus (FCoV; Alphacoronavirus 1) antibody in immunohistochemistry. Mesangioproliferative glomerulonephritis was observed, and periodic acid-Schiff-positive deposits were observed along glomerular capillary walls. These deposits stained pale red with periodic acid-methenamine silver stain and red with Masson trichrome stain, and were also observed in the mesangial matrix. In affected glomeruli, glomerular capillary walls and mesangial areas were positive for anti-ferret immunoglobulin G. By electron microscopy, subepithelial and mesangial electron-dense deposits were observed consistent with immune complex deposition. The deposition of immune complexes may have been associated with FCoV infection. CI - © 2015 The Author(s). FAU - Fujii, Yuta AU - Fujii Y AD - Preclinical Research Laboratories, Sumitomo Dainippon Pharma Co. Ltd., Konohana-ku, Osaka, Japan yuta-fujii@ds-pharma.co.jp. FAU - Tochitani, Tomoaki AU - Tochitani T AD - Preclinical Research Laboratories, Sumitomo Dainippon Pharma Co. Ltd., Konohana-ku, Osaka, Japan. FAU - Kouchi, Mami AU - Kouchi M AD - Preclinical Research Laboratories, Sumitomo Dainippon Pharma Co. Ltd., Konohana-ku, Osaka, Japan. FAU - Matsumoto, Izumi AU - Matsumoto I AD - Preclinical Research Laboratories, Sumitomo Dainippon Pharma Co. Ltd., Konohana-ku, Osaka, Japan. FAU - Yamada, Toru AU - Yamada T AD - Preclinical Research Laboratories, Sumitomo Dainippon Pharma Co. Ltd., Konohana-ku, Osaka, Japan. FAU - Funabashi, Hitoshi AU - Funabashi H AD - Preclinical Research Laboratories, Sumitomo Dainippon Pharma Co. Ltd., Konohana-ku, Osaka, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20150828 PL - United States TA - J Vet Diagn Invest JT - Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc JID - 9011490 SB - IM MH - Animals MH - Bone Marrow/pathology MH - Coronavirus Infections/complications/diagnosis/pathology/*veterinary MH - Coronavirus, Feline/immunology/*isolation & purification MH - Diagnosis, Differential MH - *Ferrets MH - Glomerulonephritis/complications/diagnosis/pathology/*veterinary MH - Immunohistochemistry/veterinary MH - Japan MH - Liver/pathology MH - Lymph Nodes/pathology MH - Male MH - Spleen/pathology OTO - NOTNLM OT - Feline infectious peritonitis OT - ferrets OT - glomerulonephritis OT - immune complex EDAT- 2015/09/01 06:00 MHDA- 2016/05/14 06:00 CRDT- 2015/08/31 06:00 PHST- 2015/08/31 06:00 [entrez] PHST- 2015/09/01 06:00 [pubmed] PHST- 2016/05/14 06:00 [medline] AID - 1040638715599570 [pii] AID - 10.1177/1040638715599570 [doi] PST - ppublish SO - J Vet Diagn Invest. 2015 Sep;27(5):637-40. doi: 10.1177/1040638715599570. Epub 2015 Aug 28. PMID- 29299855 OWN - NLM STAT- MEDLINE DCOM- 20181211 LR - 20240727 IS - 1869-1889 (Electronic) IS - 1674-7305 (Print) IS - 1674-7305 (Linking) VI - 60 IP - 12 DP - 2017 Dec TI - The persistent prevalence and evolution of cross-family recombinant coronavirus GCCDC1 among a bat population: a two-year follow-up. PG - 1357-1363 LID - 10.1007/s11427-017-9263-6 [doi] AB - Bats are connected with the increasing numbers of emerging and re-emerging viruses that may break the species barrier and spread into the human population. Coronaviruses are one of the most common viruses discovered in bats, which were considered as the natural source of recent human-susceptible coronaviruses, i.e. SARS-COV and MERS-CoV. Our previous study reported the discovery of a bat-derived putative cross-family recombinant coronavirus with a reovirus gene p10, named as Ro-BatCoV GCCDC1. In this report, through a two-year follow-up of a special bat population in one specific cave of south China, we illustrate that Ro-BatCoV GCCDC1 persistently circulates among bats. Notably, through the longitudinal observation, we identified the dynamic evolution of Ro-BatCoV GCCDC1 in bats represented by continuously recombination events. Our study provides the first glimpse of the virus evolution in one longitudinally observed bat population cohort and underlines the surveillance and pre-warning of potential interspecies transmittable viruses in bats. FAU - Obameso, Joseph O AU - Obameso JO AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Li, Hong AU - Li H AD - Yunnan Provincial Center for Disease Control and Prevention, Kunming, 650022, China. FAU - Jia, Hao AU - Jia H AD - College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China. AD - Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. FAU - Han, Min AU - Han M AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. FAU - Zhu, Shiyan AU - Zhu S AD - College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China. AD - Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. FAU - Huang, Canping AU - Huang C AD - Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. FAU - Zhao, Yuhui AU - Zhao Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. FAU - Zhao, Min AU - Zhao M AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Bai, Yu AU - Bai Y AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Yuan, Fei AU - Yuan F AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. FAU - Zhao, Honglan AU - Zhao H AD - Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. FAU - Peng, Xia AU - Peng X AD - Yunnan Provincial Center for Disease Control and Prevention, Kunming, 650022, China. FAU - Xu, Wen AU - Xu W AD - Yunnan Provincial Center for Disease Control and Prevention, Kunming, 650022, China. FAU - Tan, Wenjie AU - Tan W AD - Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. FAU - Zhao, Yingze AU - Zhao Y AD - Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, Hong Kong, China. FAU - Liu, William J AU - Liu WJ AD - College of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, China. AD - Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. FAU - Lu, Lin AU - Lu L AD - Yunnan Provincial Center for Disease Control and Prevention, Kunming, 650022, China. lulin@yncdc.cn. FAU - Gao, George F AU - Gao GF AD - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. gaofu@chinacdc.cn. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. gaofu@chinacdc.cn. AD - Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. gaofu@chinacdc.cn. LA - eng PT - Journal Article DEP - 20171201 PL - China TA - Sci China Life Sci JT - Science China. Life sciences JID - 101529880 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - China/epidemiology MH - Chiroptera/*virology MH - Coronavirus/*classification/genetics/isolation & purification MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - *Evolution, Molecular MH - Follow-Up Studies MH - Genes, Viral/genetics MH - Genome, Viral/genetics MH - *Phylogeny MH - Prevalence MH - RNA, Viral/genetics MH - *Recombination, Genetic MH - Sequence Analysis, DNA PMC - PMC7088801 OTO - NOTNLM OT - bat population OT - coronavirus OT - evolution EDAT- 2018/01/05 06:00 MHDA- 2018/12/12 06:00 PMCR- 2020/03/23 CRDT- 2018/01/05 06:00 PHST- 2017/09/11 00:00 [received] PHST- 2017/10/23 00:00 [accepted] PHST- 2018/01/05 06:00 [pubmed] PHST- 2018/12/12 06:00 [medline] PHST- 2018/01/05 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s11427-017-9263-6 [pii] AID - 9263 [pii] AID - 10.1007/s11427-017-9263-6 [doi] PST - ppublish SO - Sci China Life Sci. 2017 Dec;60(12):1357-1363. doi: 10.1007/s11427-017-9263-6. Epub 2017 Dec 1. PMID- 27342195 OWN - NLM STAT- MEDLINE DCOM- 20170731 LR - 20190111 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 13 DP - 2016 Jun 24 TI - Alphacoronavirus in urban Molossidae and Phyllostomidae bats, Brazil. PG - 110 LID - 10.1186/s12985-016-0569-4 [doi] LID - 110 AB - BACKGROUND: Bats have been implicated as the main reservoir of coronavirus (CoV). Thus the role of these hosts on the evolution and spread of CoVs currently deserve the attention of emerging diseases surveillance programs. On the view of the interest on and importance of CoVs in bats the occurrence and molecular characterization of CoV were conducted in bats from Brazil. FINDINGS: Three hundred five enteric contents of 29 bat species were tested using a panCoV nested RT-PCR. Nine specimens were positive and eight was suitable for RdRp gene sequencing. RdRp gene phylogeny showed that all CoVs strains from this study cluster in Alphacoronavirus genus, with one Molossidae and one Phlyllostomidae-CoV specific groups. Phylogenetic analyses of two S gene sequences showed a large diversity within the Alphacoronavirus genus. CONCLUSIONS: This study indicated a CoV-to-host specificity and draws attention for CoV detection in Cynomops sp, a potential new reservoir. The phylogenetic analyses indicate that diversity of CoV in bats is higher than previously known. FAU - Asano, Karen Miyuki AU - Asano KM AD - Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil. karen.asano@gmail.com. AD - Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil. karen.asano@gmail.com. FAU - Hora, Aline Santana AU - Hora AS AD - Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil. FAU - Scheffer, Karin Côrrea AU - Scheffer KC AD - Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil. FAU - Fahl, Willian Oliveira AU - Fahl WO AD - Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil. FAU - Iamamoto, Keila AU - Iamamoto K AD - Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil. FAU - Mori, Enio AU - Mori E AD - Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil. FAU - Brandão, Paulo Eduardo AU - Brandão PE AD - Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160624 PL - England TA - Virol J JT - Virology journal JID - 101231645 SB - IM EIN - Virol J. 2016 Jul 7;13(1):124. doi: 10.1186/s12985-016-0581-8. PMID: 27388456 MH - Animals MH - Brazil MH - Chiroptera/*virology MH - Coronavirus/classification/genetics/*isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - Genome, Viral MH - Phylogeny PMC - PMC4920988 OTO - NOTNLM OT - Bat OT - Coronavirus OT - Molossidae OT - Phyllostomidae EDAT- 2016/06/28 06:00 MHDA- 2017/08/02 06:00 PMCR- 2016/06/24 CRDT- 2016/06/26 06:00 PHST- 2016/04/20 00:00 [received] PHST- 2016/06/21 00:00 [accepted] PHST- 2016/06/26 06:00 [entrez] PHST- 2016/06/28 06:00 [pubmed] PHST- 2017/08/02 06:00 [medline] PHST- 2016/06/24 00:00 [pmc-release] AID - 10.1186/s12985-016-0569-4 [pii] AID - 569 [pii] AID - 10.1186/s12985-016-0569-4 [doi] PST - epublish SO - Virol J. 2016 Jun 24;13:110. doi: 10.1186/s12985-016-0569-4. PMID- 26928836 OWN - NLM STAT- MEDLINE DCOM- 20170509 LR - 20181113 IS - 1476-1645 (Electronic) IS - 0002-9637 (Print) IS - 0002-9637 (Linking) VI - 94 IP - 5 DP - 2016 May 4 TI - Diversity and Evolutionary Histories of Human Coronaviruses NL63 and 229E Associated with Acute Upper Respiratory Tract Symptoms in Kuala Lumpur, Malaysia. PG - 1058-64 LID - 10.4269/ajtmh.15-0810 [doi] AB - The human alphacoronaviruses HCoV-NL63 and HCoV-229E are commonly associated with upper respiratory tract infections (URTI). Information on their molecular epidemiology and evolutionary dynamics in the tropical region of southeast Asia however is limited. Here, we analyzed the phylogenetic, temporal distribution, population history, and clinical manifestations among patients infected with HCoV-NL63 and HCoV-229E. Nasopharyngeal swabs were collected from 2,060 consenting adults presented with acute URTI symptoms in Kuala Lumpur, Malaysia, between 2012 and 2013. The presence of HCoV-NL63 and HCoV-229E was detected using multiplex polymerase chain reaction (PCR). The spike glycoprotein, nucleocapsid, and 1a genes were sequenced for phylogenetic reconstruction and Bayesian coalescent inference. A total of 68/2,060 (3.3%) subjects were positive for human alphacoronavirus; HCoV-NL63 and HCoV-229E were detected in 45 (2.2%) and 23 (1.1%) patients, respectively. A peak in the number of HCoV-NL63 infections was recorded between June and October 2012. Phylogenetic inference revealed that 62.8% of HCoV-NL63 infections belonged to genotype B, 37.2% was genotype C, while all HCoV-229E sequences were clustered within group 4. Molecular dating analysis indicated that the origin of HCoV-NL63 was dated to 1921, before it diverged into genotype A (1975), genotype B (1996), and genotype C (2003). The root of the HCoV-229E tree was dated to 1955, before it diverged into groups 1-4 between the 1970s and 1990s. The study described the seasonality, molecular diversity, and evolutionary dynamics of human alphacoronavirus infections in a tropical region. CI - © The American Society of Tropical Medicine and Hygiene. FAU - Al-Khannaq, Maryam Nabiel AU - Al-Khannaq MN AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. FAU - Ng, Kim Tien AU - Ng KT AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. FAU - Oong, Xiang Yong AU - Oong XY AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. FAU - Pang, Yong Kek AU - Pang YK AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. FAU - Takebe, Yutaka AU - Takebe Y AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. FAU - Chook, Jack Bee AU - Chook JB AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. FAU - Hanafi, Nik Sherina AU - Hanafi NS AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. FAU - Kamarulzaman, Adeeba AU - Kamarulzaman A AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. FAU - Tee, Kok Keng AU - Tee KK AD - Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan; School of Medicine, Yokohama City University, Kanagawa, Japan; Department of Health Sciences, Faculty of Health and Life Sciences, Management and Science University, Selangor, Malaysia; Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia k2tee@um.edu.my. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160229 PL - United States TA - Am J Trop Med Hyg JT - The American journal of tropical medicine and hygiene JID - 0370507 RN - 0 (Viral Proteins) SB - IM MH - Common Cold/*epidemiology/*virology MH - Coronavirus 229E, Human/*genetics MH - Coronavirus Infections/*epidemiology/*virology MH - Coronavirus NL63, Human/*genetics MH - Gene Expression Regulation, Viral MH - Humans MH - Malaysia/epidemiology MH - Phylogeny MH - Viral Proteins/genetics/metabolism PMC - PMC4856603 EDAT- 2016/03/02 06:00 MHDA- 2017/05/10 06:00 PMCR- 2017/05/04 CRDT- 2016/03/02 06:00 PHST- 2015/11/12 00:00 [received] PHST- 2016/01/13 00:00 [accepted] PHST- 2016/03/02 06:00 [entrez] PHST- 2016/03/02 06:00 [pubmed] PHST- 2017/05/10 06:00 [medline] PHST- 2017/05/04 00:00 [pmc-release] AID - ajtmh.15-0810 [pii] AID - 10.4269/ajtmh.15-0810 [doi] PST - ppublish SO - Am J Trop Med Hyg. 2016 May 4;94(5):1058-64. doi: 10.4269/ajtmh.15-0810. Epub 2016 Feb 29. PMID- 29237834 OWN - NLM STAT- MEDLINE DCOM- 20180417 LR - 20221207 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 5 DP - 2018 Mar 1 TI - Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1. LID - 10.1128/JVI.01896-17 [doi] LID - e01896-17 AB - Porcine epidemic diarrhea virus (PEDV), an enteropathogenic Alphacoronavirus, has caused enormous economic losses in the pork industry. Nonstructural protein 1 (nsp1) is a characteristic feature of alpha- and betacoronaviruses, which exhibits both functional conservation and mechanistic diversity in inhibiting host gene expression and antiviral responses. However, the detailed structure and molecular mechanisms underlying the Alphacoronavirus nsp1 inhibition of host gene expression remain unclear. Here, we report the first full-length crystal structure of Alphacoronavirus nsp1 from PEDV. The structure displays a six-stranded β-barrel fold in the middle of two α-helices. The core structure of PEDV nsp1 shows high similarity to those of severe acute respiratory syndrome coronavirus (SARS-CoV) nsp1 and transmissible gastroenteritis virus (TGEV) nsp1, despite its low degree of sequence homology. Using ribopuromycylation and Renilla luciferase reporter assays, we showed that PEDV nsp1 can dramatically inhibit general host gene expression. Furthermore, three motifs (amino acids [aa] 67 to 71, 78 to 85, and 103 to 110) of PEDV nsp1 create a stable functional region for inhibiting protein synthesis, differing considerably from Betacoronavirus nsp1. These results elucidate the detailed structural basis through which PEDV nsp1 inhibits host gene expression, providing insight into the development of a new attenuated vaccine with nsp1 modifications.IMPORTANCE Porcine epidemic diarrhea virus (PEDV) has led to tremendous economic losses in the global swine industry. PEDV nsp1 plays a crucial role in inhibiting host gene expression, but its functional mechanism remains unclear. Here, we report the full-length structure of PEDV nsp1, the first among coronaviruses to be reported. The 1.25-Å resolution crystal structure of PEDV nsp1 shows high similarity to severe acute respiratory syndrome coronavirus (SARS-CoV) nsp1(13-128) and transmissible gastroenteritis virus (TGEV) nsp1(1-104), despite a lack of sequence homology. Structural and biochemical characterization demonstrated that PEDV nsp1 possesses a stable functional region for inhibition of host protein synthesis, which is formed by loops at residues 67 to 71, 78 to 85, and 103 to 110. The different functional regions in PEDV nsp1 and SARS-CoV nsp1 may explain their distinct mechanisms. Importantly, our structural data are conducive to understanding the mechanism of PEDV nsp1 inhibition of the expression of host genes and may aid in the development of a new attenuated vaccine. CI - Copyright © 2018 American Society for Microbiology. FAU - Shen, Zhou AU - Shen Z AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Ye, Gang AU - Ye G AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Deng, Feng AU - Deng F AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Wang, Gang AU - Wang G AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Cui, Min AU - Cui M AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Fang, Liurong AU - Fang L AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Xiao, Shaobo AU - Xiao S AUID- ORCID: 0000-0003-0023-9188 AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. FAU - Fu, Zhen F AU - Fu ZF AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. AD - Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA. FAU - Peng, Guiqing AU - Peng G AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China penggq@mail.hzau.edu.cn. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. AD - The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180212 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Vaccines, Attenuated) RN - 0 (Viral Nonstructural Proteins) RN - EC 2.7.7.48 (Nsp1 protein, SARS coronavirus) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Amino Acid Sequence MH - Animals MH - Cell Line MH - Coronavirus/genetics MH - Coronavirus Infections/prevention & control/virology MH - Crystallography, X-Ray MH - *Gene Expression Regulation MH - HEK293 Cells MH - *Host-Pathogen Interactions/genetics/physiology MH - Humans MH - Models, Molecular MH - Porcine epidemic diarrhea virus/*chemistry/genetics MH - Protein Folding MH - Protein Structure, Tertiary MH - RNA-Dependent RNA Polymerase MH - Severe acute respiratory syndrome-related coronavirus/chemistry/genetics MH - Sequence Alignment MH - Sequence Homology MH - Swine MH - Swine Diseases/prevention & control/virology MH - Transmissible gastroenteritis virus/chemistry/genetics MH - Vaccines, Attenuated MH - Viral Nonstructural Proteins/*chemistry/classification/genetics/metabolism PMC - PMC5809747 OTO - NOTNLM OT - C terminus OT - host gene expression inhibition OT - nonstructural protein 1 OT - porcine epidemic diarrhea virus OT - structure EDAT- 2017/12/15 06:00 MHDA- 2018/04/18 06:00 PMCR- 2018/08/12 CRDT- 2017/12/15 06:00 PHST- 2017/11/01 00:00 [received] PHST- 2017/11/29 00:00 [accepted] PHST- 2017/12/15 06:00 [pubmed] PHST- 2018/04/18 06:00 [medline] PHST- 2017/12/15 06:00 [entrez] PHST- 2018/08/12 00:00 [pmc-release] AID - JVI.01896-17 [pii] AID - 01896-17 [pii] AID - 10.1128/JVI.01896-17 [doi] PST - epublish SO - J Virol. 2018 Feb 12;92(5):e01896-17. doi: 10.1128/JVI.01896-17. Print 2018 Mar 1. PMID- 27009949 OWN - NLM STAT- MEDLINE DCOM- 20170428 LR - 20201215 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 90 IP - 11 DP - 2016 Jun 1 TI - Mutagenesis of Coronavirus nsp14 Reveals Its Potential Role in Modulation of the Innate Immune Response. PG - 5399-5414 LID - 10.1128/JVI.03259-15 [doi] AB - Coronavirus (CoV) nonstructural protein 14 (nsp14) is a 60-kDa protein encoded by the replicase gene that is part of the replication-transcription complex. It is a bifunctional enzyme bearing 3'-to-5' exoribonuclease (ExoN) and guanine-N7-methyltransferase (N7-MTase) activities. ExoN hydrolyzes single-stranded RNAs and double-stranded RNAs (dsRNAs) and is part of a proofreading system responsible for the high fidelity of CoV replication. nsp14 N7-MTase activity is required for viral mRNA cap synthesis and prevents the recognition of viral mRNAs as "non-self" by the host cell. In this work, a set of point mutants affecting different motifs within the ExoN domain of nsp14 was generated, using transmissible gastroenteritis virus as a model of Alphacoronavirus Mutants lacking ExoN activity were nonviable despite being competent in both viral RNA and protein synthesis. A specific mutation within zinc finger 1 (ZF-C) led to production of a viable virus with growth and viral RNA synthesis kinetics similar to that of the parental virus. Mutant recombinant transmissible gastroenteritis virus (TGEV) ZF-C (rTGEV-ZF-C) caused decreased cytopathic effect and apoptosis compared with the wild-type virus and reduced levels of dsRNA accumulation at late times postinfection. Consequently, the mutant triggered a reduced antiviral response, which was confirmed by evaluating different stages of the dsRNA-induced antiviral pathway. The expression of beta interferon (IFN-β), tumor necrosis factor (TNF), and interferon-stimulated genes in cells infected with mutant rTGEV-ZF-C was reduced compared to the levels seen with the parental virus. Overall, our data revealed a potential role for CoV nsp14 in modulation of the innate immune response. IMPORTANCE: The innate immune response is the first line of antiviral defense that culminates in the synthesis of interferon and proinflammatory cytokines to control viral replication. CoVs have evolved several mechanisms to counteract the innate immune response at different levels, but the role of CoV-encoded ribonucleases in preventing activation of the dsRNA-induced antiviral response has not been described to date. The introduction of a mutation in zinc finger 1 of the ExoN domain of nsp14 led to production of a virus that induced a weak antiviral response, most likely due to the accumulation of lower levels of dsRNA in the late phases of infection. These observations allowed us to propose a novel role for CoV nsp14 ExoN activity in counteracting the antiviral response, which could serve as a novel target for the design of antiviral strategies. CI - Copyright © 2016, American Society for Microbiology. All Rights Reserved. FAU - Becares, Martina AU - Becares M AD - Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain. FAU - Pascual-Iglesias, Alejandro AU - Pascual-Iglesias A AD - Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain. FAU - Nogales, Aitor AU - Nogales A AD - Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain. FAU - Sola, Isabel AU - Sola I AD - Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain. FAU - Enjuanes, Luis AU - Enjuanes L AD - Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain L.Enjuanes@cnb.csic.es. FAU - Zuñiga, Sonia AU - Zuñiga S AD - Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain. LA - eng GR - P01 AI060699/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20160512 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (RNA, Viral) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (Viral Nonstructural Proteins) RN - 77238-31-4 (Interferon-beta) RN - EC 2.1.1.- (Methyltransferases) RN - EC 3.1.- (Exoribonucleases) SB - IM MH - Cytopathogenic Effect, Viral MH - Exoribonucleases/genetics/metabolism MH - Humans MH - *Immunity, Innate MH - *Immunomodulation MH - Interferon-beta/genetics MH - Methyltransferases/genetics/metabolism MH - *Mutagenesis MH - Point Mutation MH - RNA, Viral MH - Transmissible gastroenteritis virus/*genetics/*physiology MH - Tumor Necrosis Factor-alpha/genetics MH - Viral Nonstructural Proteins/*genetics/*metabolism MH - Virus Replication MH - Zinc Fingers/genetics PMC - PMC4934755 EDAT- 2016/03/25 06:00 MHDA- 2017/04/30 06:00 PMCR- 2016/11/12 CRDT- 2016/03/25 06:00 PHST- 2015/12/30 00:00 [received] PHST- 2016/03/15 00:00 [accepted] PHST- 2016/03/25 06:00 [entrez] PHST- 2016/03/25 06:00 [pubmed] PHST- 2017/04/30 06:00 [medline] PHST- 2016/11/12 00:00 [pmc-release] AID - JVI.03259-15 [pii] AID - 03259-15 [pii] AID - 10.1128/JVI.03259-15 [doi] PST - epublish SO - J Virol. 2016 May 12;90(11):5399-5414. doi: 10.1128/JVI.03259-15. Print 2016 Jun 1. PMID- 25589635 OWN - NLM STAT- MEDLINE DCOM- 20150526 LR - 20181113 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 6 DP - 2015 Mar TI - Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains. PG - 3332-42 LID - 10.1128/JVI.03196-14 [doi] AB - Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are economically important swine enteropathogenic coronaviruses. These two viruses belong to two distinct species of the Alphacoronavirus genus within Coronaviridae and induce similar clinical signs and pathological lesions in newborn piglets, but they are presumed to be antigenically distinct. In the present study, two-way antigenic cross-reactivity examinations between the prototype PEDV CV777 strain, three distinct U.S. PEDV strains (the original highly virulent PC22A, S indel Iowa106, and S 197del PC177), and two representative U.S. TGEV strains (Miller and Purdue) were conducted by cell culture immunofluorescent (CCIF) and viral neutralization (VN) assays. None of the pig TGEV antisera neutralized PEDV and vice versa. One-way cross-reactions were observed by CCIF between TGEV Miller hyperimmune pig antisera and all PEDV strains. Enzyme-linked immunosorbent assays, immunoblotting using monoclonal antibodies and Escherichia coli-expressed recombinant PEDV and TGEV nucleocapsid (N) proteins, and sequence analysis suggested at least one epitope on the N-terminal region of PEDV/TGEV N protein that contributed to this cross-reactivity. Biologically, PEDV strain CV777 induced greater cell fusion in Vero cells than did U.S. PEDV strains. Consistent with the reported genetic differences, the results of CCIF and VN assays also revealed higher antigenic variation between PEDV CV777 and U.S. strains. IMPORTANCE: Evidence of antigenic cross-reactivity between porcine enteric coronaviruses, PEDV and TGEV, in CCIF assays supports the idea that these two species are evolutionarily related, but they are distinct species defined by VN assays. Identification of PEDV- or TGEV-specific antigenic regions allows the development of more specific immunoassays for each virus. Antigenic and biologic variations between the prototype and current PEDV strains could explain, at least partially, the recurrence of PEDV epidemics. Information on the conserved antigenicity among PEDV strains is important for the development of PEDV vaccines to protect swine from current highly virulent PEDV infections. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Lin, Chun-Ming AU - Lin CM AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. FAU - Gao, Xiang AU - Gao X AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. FAU - Oka, Tomoichiro AU - Oka T AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. FAU - Vlasova, Anastasia N AU - Vlasova AN AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. FAU - Esseili, Malak A AU - Esseili MA AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA. FAU - Wang, Qiuhong AU - Wang Q AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA saif.2@osu.edu wang.655@osu.edu. FAU - Saif, Linda J AU - Saif LJ AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio, USA saif.2@osu.edu wang.655@osu.edu. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150114 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Antibodies, Viral) SB - IM MH - Amino Acid Sequence MH - Animals MH - Antibodies, Viral/*immunology MH - Antigenic Variation MH - Coronavirus Infections/immunology/*veterinary/virology MH - Cross Reactions MH - Gastroenteritis, Transmissible, of Swine/immunology/virology MH - Molecular Sequence Data MH - Porcine epidemic diarrhea virus/chemistry/classification/genetics/*immunology MH - Sequence Alignment MH - Swine MH - Swine Diseases/*immunology/virology MH - Transmissible gastroenteritis virus/chemistry/classification/genetics/*immunology PMC - PMC4337547 EDAT- 2015/01/16 06:00 MHDA- 2015/05/27 06:00 PMCR- 2015/09/15 CRDT- 2015/01/16 06:00 PHST- 2015/01/16 06:00 [entrez] PHST- 2015/01/16 06:00 [pubmed] PHST- 2015/05/27 06:00 [medline] PHST- 2015/09/15 00:00 [pmc-release] AID - JVI.03196-14 [pii] AID - 03196-14 [pii] AID - 10.1128/JVI.03196-14 [doi] PST - ppublish SO - J Virol. 2015 Mar;89(6):3332-42. doi: 10.1128/JVI.03196-14. Epub 2015 Jan 14. PMID- 27287409 OWN - NLM STAT- MEDLINE DCOM- 20170915 LR - 20221207 IS - 1097-4180 (Electronic) IS - 1074-7613 (Print) IS - 1074-7613 (Linking) VI - 44 IP - 6 DP - 2016 Jun 21 TI - Airway Memory CD4(+) T Cells Mediate Protective Immunity against Emerging Respiratory Coronaviruses. PG - 1379-91 LID - S1074-7613(16)30160-1 [pii] LID - 10.1016/j.immuni.2016.05.006 [doi] AB - Two zoonotic coronaviruses (CoVs)-SARS-CoV and MERS-CoV-have crossed species to cause severe human respiratory disease. Here, we showed that induction of airway memory CD4(+) T cells specific for a conserved epitope shared by SARS-CoV and MERS-CoV is a potential strategy for developing pan-coronavirus vaccines. Airway memory CD4(+) T cells differed phenotypically and functionally from lung-derived cells and were crucial for protection against both CoVs in mice. Protection was dependent on interferon-γ and required early induction of robust innate and virus-specific CD8(+) T cell responses. The conserved epitope was also recognized in SARS-CoV- and MERS-CoV-infected human leukocyte antigen DR2 and DR3 transgenic mice, indicating potential relevance in human populations. Additionally, this epitope was cross-protective between human and bat CoVs, the progenitors for many human CoVs. Vaccine strategies that induce airway memory CD4(+) T cells targeting conserved epitopes might have broad applicability in the context of new CoVs and other respiratory virus outbreaks. CI - Copyright © 2016 Elsevier Inc. All rights reserved. FAU - Zhao, Jincun AU - Zhao J AD - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China; Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. Electronic address: zhaojincun@gird.cn. FAU - Zhao, Jingxian AU - Zhao J AD - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. FAU - Mangalam, Ashutosh K AU - Mangalam AK AD - Department of Pathology, University of Iowa, Iowa City, IA 52242, USA. FAU - Channappanavar, Rudragouda AU - Channappanavar R AD - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. FAU - Fett, Craig AU - Fett C AD - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. FAU - Meyerholz, David K AU - Meyerholz DK AD - Department of Pathology, University of Iowa, Iowa City, IA 52242, USA. FAU - Agnihothram, Sudhakar AU - Agnihothram S AD - Department of Microbiology and Immunology and Department of Epidemiology, University of North Carolina, Chapel Hill, NC 27599, USA. FAU - Baric, Ralph S AU - Baric RS AD - Department of Microbiology and Immunology and Department of Epidemiology, University of North Carolina, Chapel Hill, NC 27599, USA. FAU - David, Chella S AU - David CS AD - Department of Immunology, Mayo Clinic, Rochester, MI 55905, USA. FAU - Perlman, Stanley AU - Perlman S AD - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA. Electronic address: stanley-perlman@uiowa.edu. LA - eng GR - P01 AI060699/AI/NIAID NIH HHS/United States GR - R01 AI091322/AI/NIAID NIH HHS/United States GR - U19 AI100625/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20160607 PL - United States TA - Immunity JT - Immunity JID - 9432918 RN - 0 (Antigens, Viral) RN - 0 (Epitopes, T-Lymphocyte) RN - 0 (Viral Vaccines) RN - 82115-62-6 (Interferon-gamma) SB - IM MH - Animals MH - Antigens, Viral/immunology MH - CD4-Positive T-Lymphocytes/*immunology/virology MH - CD8-Positive T-Lymphocytes/*immunology/virology MH - Cells, Cultured MH - Coronavirus Infections/*immunology MH - Cross Reactions MH - Epitopes, T-Lymphocyte/immunology MH - Humans MH - Immunity MH - Immunologic Memory MH - Interferon-gamma/metabolism MH - Mice MH - Mice, Inbred Strains MH - Respiratory System/*immunology MH - Severe acute respiratory syndrome-related coronavirus/*immunology MH - Severe Acute Respiratory Syndrome/*immunology MH - Vaccination MH - Viral Vaccines/*immunology MH - Virion/immunology PMC - PMC4917442 MID - NIHMS786479 EDAT- 2016/06/12 06:00 MHDA- 2017/09/16 06:00 PMCR- 2016/06/07 CRDT- 2016/06/12 06:00 PHST- 2015/11/19 00:00 [received] PHST- 2016/02/14 00:00 [revised] PHST- 2016/03/08 00:00 [accepted] PHST- 2016/06/12 06:00 [entrez] PHST- 2016/06/12 06:00 [pubmed] PHST- 2017/09/16 06:00 [medline] PHST- 2016/06/07 00:00 [pmc-release] AID - S1074-7613(16)30160-1 [pii] AID - 10.1016/j.immuni.2016.05.006 [doi] PST - ppublish SO - Immunity. 2016 Jun 21;44(6):1379-91. doi: 10.1016/j.immuni.2016.05.006. Epub 2016 Jun 7. PMID- 30103259 OWN - NLM STAT- MEDLINE DCOM- 20190311 LR - 20200422 IS - 1865-1682 (Electronic) IS - 1865-1674 (Print) IS - 1865-1674 (Linking) VI - 66 IP - 1 DP - 2019 Jan TI - Isolation and characterization of a highly pathogenic strain of Porcine enteric alphacoronavirus causing watery diarrhoea and high mortality in newborn piglets. PG - 119-130 LID - 10.1111/tbed.12992 [doi] AB - Porcine enteric alphacoronavirus (PEAV) was first discovered in China in February 2017, and the origin and virulence of this novel porcine coronavirus were not fully characterized. Here, we isolated a strain of PEAV, named GDS04 that is identified by immunofluorescence and typical crown-shaped particles observed with electron microscopy. Genomic analysis reveals that PEAV GDS04 shares a close relationship with SADS-CoV and SeACoV. Furthermore, newborn piglets orally challenged with PEAV GDS04 developed typical clinical symptoms as watery diarrhoea in neonatal piglets. Viral RNA was detected in faeces and various tissues of the infected piglets. Moreover, macroscopic and microscopic lesions in whole intestinal tract were observed, and viral antigen could be detected in the small intestines by immunohistochemical staining and electron microscopy. Importantly, the mortality rate of inoculated-newborn piglets was 100% and half of the cohabiting piglets died. Collectively, we demonstrate that PEAV is highly pathogenic in newborn piglets. CI - © 2018 Blackwell Verlag GmbH. FAU - Xu, Zhichao AU - Xu Z AUID- ORCID: 0000-0002-5697-5734 AD - State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China. FAU - Zhang, Yun AU - Zhang Y AD - State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China. FAU - Gong, Lang AU - Gong L AD - State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China. FAU - Huang, Licheng AU - Huang L AD - State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China. FAU - Lin, Ying AU - Lin Y AD - State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China. FAU - Qin, Jianru AU - Qin J AD - State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China. FAU - Du, Yunping AU - Du Y AD - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd, Xinxing, China. FAU - Zhou, Qingfeng AU - Zhou Q AD - Guangdong Wen's Group Academy, Guangdong Wen's Foodstuffs Group Co., Ltd, Xinxing, China. FAU - Xue, Chunyi AU - Xue C AD - State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China. FAU - Cao, Yongchang AU - Cao Y AUID- ORCID: 0000-0002-0311-5474 AD - State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, China. LA - eng SI - GENBANK/MF167434.1 SI - GENBANK/MH697599 SI - GENBANK/MF167434 GR - 2016YFD0500101/National Key Research and Development Program/ GR - 31741118/National Natural Science Foundation/ GR - 2018B030314003/Guangdong Natural Science Foundation/ PT - Journal Article DEP - 20180910 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 RN - 0 (Antigens, Viral) RN - 0 (RNA, Viral) SB - IM MH - Alphacoronavirus/isolation & purification/*physiology MH - Animals MH - Antigens, Viral/analysis MH - China MH - Coronavirus Infections/mortality/*veterinary/virology MH - Diarrhea/*veterinary/virology MH - Feces/virology MH - Intestines/pathology MH - RNA, Viral/analysis MH - Swine MH - Swine Diseases/*mortality/*virology PMC - PMC7168553 OTO - NOTNLM OT - Porcine enteric alphacoronavirus (PEAV) OT - newborn piglets OT - pathogenicity COIS- The authors declare that they have no conflict of interest. EDAT- 2018/08/14 06:00 MHDA- 2019/03/12 06:00 PMCR- 2020/04/20 CRDT- 2018/08/14 06:00 PHST- 2018/04/09 00:00 [received] PHST- 2018/08/03 00:00 [revised] PHST- 2018/08/06 00:00 [accepted] PHST- 2018/08/14 06:00 [pubmed] PHST- 2019/03/12 06:00 [medline] PHST- 2018/08/14 06:00 [entrez] PHST- 2020/04/20 00:00 [pmc-release] AID - TBED12992 [pii] AID - 10.1111/tbed.12992 [doi] PST - ppublish SO - Transbound Emerg Dis. 2019 Jan;66(1):119-130. doi: 10.1111/tbed.12992. Epub 2018 Sep 10. PMID- 26678874 OWN - NLM STAT- MEDLINE DCOM- 20160119 LR - 20220311 IS - 1095-9203 (Electronic) IS - 0036-8075 (Linking) VI - 351 IP - 6268 DP - 2016 Jan 1 TI - Co-circulation of three camel coronavirus species and recombination of MERS-CoVs in Saudi Arabia. PG - 81-4 LID - 10.1126/science.aac8608 [doi] AB - Outbreaks of Middle East respiratory syndrome (MERS) raise questions about the prevalence and evolution of the MERS coronavirus (CoV) in its animal reservoir. Our surveillance in Saudi Arabia in 2014 and 2015 showed that viruses of the MERS-CoV species and a human CoV 229E-related lineage co-circulated at high prevalence, with frequent co-infections in the upper respiratory tract of dromedary camels. viruses of the betacoronavirus 1 species, we found that dromedary camels share three CoV species with humans. Several MERS-CoV lineages were present in camels, including a recombinant lineage that has been dominant since December 2014 and that subsequently led to the human outbreaks in 2015. Camels therefore serve as an important reservoir for the maintenance and diversification of the MERS-CoVs and are the source of human infections with this virus. CI - Copyright © 2016, American Association for the Advancement of Science. FAU - Sabir, Jamal S M AU - Sabir JS AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Lam, Tommy T-Y AU - Lam TT AD - State Key Laboratory of Emerging Infectious Diseases (The University of Hong Kong-Shenzhen Branch), Shenzhen Third People's Hospital, Shenzhen, China. Shantou University-The University of Hong Kong Joint Institute of Virology, Shantou University, Shantou, China. Centre of Influenza Research and State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Ahmed, Mohamed M M AU - Ahmed MM AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Department of Nucleic Acids Research, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Borg El-Arab, Post Office Box 21934, Alexandria, Egypt. FAU - Li, Lifeng AU - Li L AD - Shantou University-The University of Hong Kong Joint Institute of Virology, Shantou University, Shantou, China. Centre of Influenza Research and State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Shen, Yongyi AU - Shen Y AD - Shantou University-The University of Hong Kong Joint Institute of Virology, Shantou University, Shantou, China. Centre of Influenza Research and State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Abo-Aba, Salah E M AU - Abo-Aba SE AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Microbial Genetics Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Giza, Egypt. FAU - Qureshi, Muhammd I AU - Qureshi MI AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Abu-Zeid, Mohamed AU - Abu-Zeid M AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. Microbial Genetics Department, Genetic Engineering and Biotechnology Division, National Research Center, Dokki, Giza, Egypt. FAU - Zhang, Yu AU - Zhang Y AD - State Key Laboratory of Emerging Infectious Diseases (The University of Hong Kong-Shenzhen Branch), Shenzhen Third People's Hospital, Shenzhen, China. Shantou University-The University of Hong Kong Joint Institute of Virology, Shantou University, Shantou, China. Centre of Influenza Research and State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Khiyami, Mohammad A AU - Khiyami MA AD - King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Alharbi, Njud S AU - Alharbi NS AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Hajrah, Nahid H AU - Hajrah NH AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Sabir, Meshaal J AU - Sabir MJ AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Mutwakil, Mohammed H Z AU - Mutwakil MH AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Kabli, Saleh A AU - Kabli SA AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Alsulaimany, Faten A S AU - Alsulaimany FA AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Obaid, Abdullah Y AU - Obaid AY AD - Department of Chemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Zhou, Boping AU - Zhou B AD - State Key Laboratory of Emerging Infectious Diseases (The University of Hong Kong-Shenzhen Branch), Shenzhen Third People's Hospital, Shenzhen, China. FAU - Smith, David K AU - Smith DK AD - Centre of Influenza Research and State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region, China. FAU - Holmes, Edward C AU - Holmes EC AD - Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Biological Sciences and Sydney Medical School, The University of Sydney, Sydney, New South Wales 2006, Australia. FAU - Zhu, Huachen AU - Zhu H AD - State Key Laboratory of Emerging Infectious Diseases (The University of Hong Kong-Shenzhen Branch), Shenzhen Third People's Hospital, Shenzhen, China. Shantou University-The University of Hong Kong Joint Institute of Virology, Shantou University, Shantou, China. Centre of Influenza Research and State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region, China. zhuhch@hku.hk yguan@hku.hk. FAU - Guan, Yi AU - Guan Y AD - Biotechnology Research Group, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. State Key Laboratory of Emerging Infectious Diseases (The University of Hong Kong-Shenzhen Branch), Shenzhen Third People's Hospital, Shenzhen, China. Shantou University-The University of Hong Kong Joint Institute of Virology, Shantou University, Shantou, China. Centre of Influenza Research and State Key Laboratory of Emerging Infectious Diseases, School of Public Health, The University of Hong Kong, Hong Kong Special Administrative Region, China. zhuhch@hku.hk yguan@hku.hk. LA - eng SI - GENBANK/KT368824 SI - GENBANK/KT368825 SI - GENBANK/KT368826 SI - GENBANK/KT368827 SI - GENBANK/KT368828 SI - GENBANK/KT368829 SI - GENBANK/KT368830 SI - GENBANK/KT368831 SI - GENBANK/KT368832 SI - GENBANK/KT368833 SI - GENBANK/KT368834 SI - GENBANK/KT368835 SI - GENBANK/KT368836 SI - GENBANK/KT368837 SI - GENBANK/KT368838 SI - GENBANK/KT368839 SI - GENBANK/KT368840 SI - GENBANK/KT368841 SI - GENBANK/KT368842 SI - GENBANK/KT368843 SI - GENBANK/KT368844 SI - GENBANK/KT368845 SI - GENBANK/KT368846 SI - GENBANK/KT368847 SI - GENBANK/KT368848 SI - GENBANK/KT368849 SI - GENBANK/KT368850 SI - GENBANK/KT368851 SI - GENBANK/KT368852 SI - GENBANK/KT368853 SI - GENBANK/KT368854 SI - GENBANK/KT368855 SI - GENBANK/KT368856 SI - GENBANK/KT368857 SI - GENBANK/KT368858 SI - GENBANK/KT368859 SI - GENBANK/KT368860 SI - GENBANK/KT368861 SI - GENBANK/KT368862 SI - GENBANK/KT368863 SI - GENBANK/KT368864 SI - GENBANK/KT368865 SI - GENBANK/KT368866 SI - GENBANK/KT368867 SI - GENBANK/KT368868 SI - GENBANK/KT368869 SI - GENBANK/KT368870 SI - GENBANK/KT368871 SI - GENBANK/KT368872 SI - GENBANK/KT368873 SI - GENBANK/KT368874 SI - GENBANK/KT368875 SI - GENBANK/KT368876 SI - GENBANK/KT368877 SI - GENBANK/KT368878 SI - GENBANK/KT368879 SI - GENBANK/KT368880 SI - GENBANK/KT368881 SI - GENBANK/KT368882 SI - GENBANK/KT368883 SI - GENBANK/KT368884 SI - GENBANK/KT368885 SI - GENBANK/KT368886 SI - GENBANK/KT368887 SI - GENBANK/KT368888 SI - GENBANK/KT368889 SI - GENBANK/KT368890 SI - GENBANK/KT368891 SI - GENBANK/KT368892 SI - GENBANK/KT368893 SI - GENBANK/KT368894 SI - GENBANK/KT368895 SI - GENBANK/KT368896 SI - GENBANK/KT368897 SI - GENBANK/KT368898 SI - GENBANK/KT368899 SI - GENBANK/KT368900 SI - GENBANK/KT368901 SI - GENBANK/KT368902 SI - GENBANK/KT368903 SI - GENBANK/KT368904 SI - GENBANK/KT368905 SI - GENBANK/KT368906 SI - GENBANK/KT368907 SI - GENBANK/KT368908 SI - GENBANK/KT368909 SI - GENBANK/KT368910 SI - GENBANK/KT368911 SI - GENBANK/KT368912 SI - GENBANK/KT368913 SI - GENBANK/KT368914 SI - GENBANK/KT368915 SI - GENBANK/KT368916 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151217 PL - United States TA - Science JT - Science (New York, N.Y.) JID - 0404511 SB - IM MH - Animals MH - Camelus/*virology MH - Coinfection/epidemiology/veterinary/*virology MH - Coronavirus Infections/epidemiology/veterinary/*virology MH - Disease Reservoirs/veterinary/*virology MH - Epidemiological Monitoring MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/classification/*genetics/*physiology MH - Molecular Sequence Data MH - Phylogeny MH - *Recombination, Genetic MH - Saudi Arabia/epidemiology EDAT- 2015/12/19 06:00 MHDA- 2016/01/20 06:00 CRDT- 2015/12/19 06:00 PHST- 2015/06/24 00:00 [received] PHST- 2015/11/12 00:00 [accepted] PHST- 2015/12/19 06:00 [entrez] PHST- 2015/12/19 06:00 [pubmed] PHST- 2016/01/20 06:00 [medline] AID - science.aac8608 [pii] AID - 10.1126/science.aac8608 [doi] PST - ppublish SO - Science. 2016 Jan 1;351(6268):81-4. doi: 10.1126/science.aac8608. Epub 2015 Dec 17. PMID- 28677421 OWN - NLM STAT- MEDLINE DCOM- 20180228 LR - 20190522 IS - 1943-4936 (Electronic) IS - 1040-6387 (Linking) VI - 29 IP - 5 DP - 2017 Sep TI - Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil. PG - 660-668 LID - 10.1177/1040638717720245 [doi] AB - We describe molecular testing for felid alphaherpesvirus 1 (FHV-1), carnivore protoparvovirus 1 (CPPV-1), feline calicivirus (FCV), alphacoronavirus 1 (feline coronavirus [FCoV]), feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), and canine distemper virus (CDV) in whole blood samples of 109 free-ranging and 68 captive neotropical felids from Brazil. Samples from 2 jaguars ( Panthera onca) and 1 oncilla ( Leopardus tigrinus) were positive for FHV-1; 2 jaguars, 1 puma ( Puma concolor), and 1 jaguarundi ( Herpairulus yagouaroundi) tested positive for CPPV-1; and 1 puma was positive for FIV. Based on comparison of 103 nucleotides of the UL24-UL25 gene, the FHV-1 sequences were 99-100% similar to the FHV-1 strain of domestic cats. Nucleotide sequences of CPPV-1 were closely related to sequences detected in other wild carnivores, comparing 294 nucleotides of the VP1 gene. The FIV nucleotide sequence detected in the free-ranging puma, based on comparison of 444 nucleotides of the pol gene, grouped with other lentiviruses described in pumas, and had 82.4% identity with a free-ranging puma from Yellowstone Park and 79.5% with a captive puma from Brazil. Our data document the circulation of FHV-1, CPPV-1, and FIV in neotropical felids in Brazil. FAU - Furtado, Mariana M AU - Furtado MM AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Taniwaki, Sueli A AU - Taniwaki SA AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - de Barros, Iracema N AU - de Barros IN AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Brandão, Paulo E AU - Brandão PE AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Catão-Dias, José L AU - Catão-Dias JL AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Cavalcanti, Sandra AU - Cavalcanti S AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Cullen, Laury AU - Cullen L AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Filoni, Claudia AU - Filoni C AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Jácomo, Anah T de Almeida AU - Jácomo ATA AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Jorge, Rodrigo S P AU - Jorge RSP AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Silva, Nairléia Dos Santos AU - Silva NDS AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Silveira, Leandro AU - Silveira L AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). FAU - Ferreira Neto, José S AU - Ferreira Neto JS AD - Departments of Preventive Veterinary Medicine and Animal Health (Furtado, Taniwaki, Barros, Brandão, Silva, Ferreira Neto), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Pathology (Catão-Dias), School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo-SP, Brazil. AD - Jaguar Conservation Fund/Instituto Onça-Pintada, Mineiros-GO, Brazil (Furtado, Jácomo, Silveira). AD - Institute for the Conservation of Neotropical Carnivores/Instituto Pró-Carnívoros, Atibaia-SP, Brazil (Cavalcanti, Jorge). AD - Institute for Ecological Research/Instituto de Pesquisas Ecológicas (IPE), Piracicaba-SP, Brazil (Cullen). AD - Department of Microbiology and Immunology, Biosciences Institute, State University of São Paulo (UNESP) Júlio de Mesquita Filho, Botucatu-SP, Brazil (Filoni). AD - Brazilian Institute for Conservation Medicine/Instituto Brasileiro para Medicina da Conservação (TRÍADE), Recife, PE, Brazil (Jorge). AD - Chico Mendes Institute for Biodiversity Conservation/ICMBio, Brasília-DF, Brazil (Jorge). LA - eng PT - Journal Article DEP - 20170705 PL - United States TA - J Vet Diagn Invest JT - Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc JID - 9011490 SB - IM MH - Animals MH - Animals, Wild MH - Animals, Zoo MH - Brazil MH - Calicivirus, Feline/genetics/isolation & purification MH - Coronavirus, Feline/genetics/isolation & purification MH - Distemper Virus, Canine/genetics/isolation & purification MH - Felidae/blood/*virology MH - Herpesviridae/genetics/isolation & purification MH - Immunodeficiency Virus, Feline/genetics/isolation & purification MH - Leukemia Virus, Feline/genetics/isolation & purification MH - Parvovirinae/genetics/isolation & purification MH - Serologic Tests/veterinary MH - Varicellovirus/genetics/isolation & purification MH - Virus Diseases/diagnosis/*veterinary/virology OTO - NOTNLM OT - Carnivore protoparvovirus 1 OT - Herpairulus yagouaroundi OT - Leopardus tigrinus OT - Panthera onca OT - Puma concolor. OT - felid alphaherpesvirus 1 OT - feline immunodeficiency virus EDAT- 2017/07/06 06:00 MHDA- 2018/03/01 06:00 CRDT- 2017/07/06 06:00 PHST- 2017/07/06 06:00 [pubmed] PHST- 2018/03/01 06:00 [medline] PHST- 2017/07/06 06:00 [entrez] AID - 10.1177/1040638717720245 [doi] PST - ppublish SO - J Vet Diagn Invest. 2017 Sep;29(5):660-668. doi: 10.1177/1040638717720245. Epub 2017 Jul 5. PMID- 30248143 OWN - NLM STAT- MEDLINE DCOM- 20190122 LR - 20240402 IS - 1553-7374 (Electronic) IS - 1553-7366 (Print) IS - 1553-7366 (Linking) VI - 14 IP - 9 DP - 2018 Sep TI - The papain-like protease determines a virulence trait that varies among members of the SARS-coronavirus species. PG - e1007296 LID - 10.1371/journal.ppat.1007296 [doi] LID - e1007296 AB - SARS-coronavirus (CoV) is a zoonotic agent derived from rhinolophid bats, in which a plethora of SARS-related, conspecific viral lineages exist. Whereas the variability of virulence among reservoir-borne viruses is unknown, it is generally assumed that the emergence of epidemic viruses from animal reservoirs requires human adaptation. To understand the influence of a viral factor in relation to interspecies spillover, we studied the papain-like protease (PLP) of SARS-CoV. This key enzyme drives the early stages of infection as it cleaves the viral polyprotein, deubiquitinates viral and cellular proteins, and antagonizes the interferon (IFN) response. We identified a bat SARS-CoV PLP, which shared 86% amino acid identity with SARS-CoV PLP, and used reverse genetics to insert it into the SARS-CoV genome. The resulting virus replicated like SARS-CoV in Vero cells but was suppressed in IFN competent MA-104 (3.7-fold), Calu-3 (2.6-fold) and human airway epithelial cells (10.3-fold). Using ectopically-expressed PLP variants as well as full SARS-CoV infectious clones chimerized for PLP, we found that a protease-independent, anti-IFN function exists in SARS-CoV, but not in a SARS-related, bat-borne virus. This PLP-mediated anti-IFN difference was seen in primate, human as well as bat cells, thus independent of the host context. The results of this study revealed that coronavirus PLP confers a variable virulence trait among members of the species SARS-CoV, and that a SARS-CoV lineage with virulent PLPs may have pre-existed in the reservoir before onset of the epidemic. FAU - Niemeyer, Daniela AU - Niemeyer D AUID- ORCID: 0000-0002-1897-6365 AD - Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany. AD - German Centre for Infection Research, associated partner Charité, Berlin, Germany. FAU - Mösbauer, Kirstin AU - Mösbauer K AD - Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Klein, Eva M AU - Klein EM AUID- ORCID: 0000-0002-5622-8202 AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany. FAU - Sieberg, Andrea AU - Sieberg A AD - Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany. FAU - Mettelman, Robert C AU - Mettelman RC AD - Department of Microbiology and Immunology, Loyola University of Chicago, Maywood, IL, United States of America. FAU - Mielech, Anna M AU - Mielech AM AD - Department of Microbiology and Immunology, Loyola University of Chicago, Maywood, IL, United States of America. FAU - Dijkman, Ronald AU - Dijkman R AUID- ORCID: 0000-0003-0320-2743 AD - Institute of Virology and Immunology, Bern & Mittelhäusern, Switzerland. AD - Department of Infectious Diseases and Pathobiology, University of Bern, Bern, Switzerland. FAU - Baker, Susan C AU - Baker SC AD - Department of Microbiology and Immunology, Loyola University of Chicago, Maywood, IL, United States of America. FAU - Drosten, Christian AU - Drosten C AD - Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany. AD - German Centre for Infection Research, associated partner Charité, Berlin, Germany. FAU - Müller, Marcel A AU - Müller MA AUID- ORCID: 0000-0003-2242-5117 AD - Institute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany. AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany. AD - German Centre for Infection Research, associated partner Charité, Berlin, Germany. LA - eng GR - R01 AI085089/AI/NIAID NIH HHS/United States GR - T32 AI007508/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20180924 PL - United States TA - PLoS Pathog JT - PLoS pathogens JID - 101238921 RN - 0 (Ubiquitin) RN - 0 (Viral Proteins) RN - 9008-11-1 (Interferons) RN - EC 3.4.22.- (Cysteine Endopeptidases) RN - EC 3.4.22.28 (Coronavirus 3C Proteases) SB - IM MH - Amino Acid Sequence MH - Animals MH - Chiroptera/virology MH - Chlorocebus aethiops MH - Coronavirus 3C Proteases MH - Cysteine Endopeptidases/genetics/*physiology MH - Disease Reservoirs/virology MH - HEK293 Cells MH - Host Specificity MH - Host-Pathogen Interactions MH - Humans MH - Interferons/antagonists & inhibitors MH - Phylogeny MH - Severe acute respiratory syndrome-related coronavirus/*enzymology/genetics/*pathogenicity MH - Sequence Homology, Amino Acid MH - Severe Acute Respiratory Syndrome/epidemiology/virology MH - Ubiquitin/metabolism MH - Vero Cells MH - Viral Proteins/genetics/*physiology MH - Virulence/genetics/physiology MH - Virus Replication/genetics/physiology MH - Zoonoses/epidemiology/virology PMC - PMC6171950 COIS- The authors have declared that no competing interests exist. EDAT- 2018/09/25 06:00 MHDA- 2019/01/23 06:00 PMCR- 2018/09/24 CRDT- 2018/09/25 06:00 PHST- 2018/04/12 00:00 [received] PHST- 2018/08/26 00:00 [accepted] PHST- 2018/10/04 00:00 [revised] PHST- 2018/09/25 06:00 [pubmed] PHST- 2019/01/23 06:00 [medline] PHST- 2018/09/25 06:00 [entrez] PHST- 2018/09/24 00:00 [pmc-release] AID - PPATHOGENS-D-18-00742 [pii] AID - 10.1371/journal.ppat.1007296 [doi] PST - epublish SO - PLoS Pathog. 2018 Sep 24;14(9):e1007296. doi: 10.1371/journal.ppat.1007296. eCollection 2018 Sep. PMID- 29377436 OWN - NLM STAT- MEDLINE DCOM- 20181015 LR - 20181015 IS - 1365-2893 (Electronic) IS - 1352-0504 (Linking) VI - 25 IP - 6 DP - 2018 Jun TI - Hepatitis E virus seroprevalence, seroincidence and seroreversion in the German adult population. PG - 752-758 LID - 10.1111/jvh.12868 [doi] AB - A steep rise in Hepatitis E diagnoses is currently being observed in Germany and other European countries. The objective of this study was (i) to assess whether this trend mirrors an increase in infection pressure or is caused by increased attention and testing and (ii) estimate individual and population-based Hepatitis E Virus (HEV) seroconversion and seroreversion rates for Germany. We measured anti-HEV IgG prevalence in 10 407 adults participating in two linked, population-representative serosurveys (total n = 12 971) conducted in 1998 and 2010. In this period, we found a moderate but statistically significant decline of overall anti-HEV IgG prevalence from 18.6% to 15.3%. At both time points, seroprevalence increased with age and peaked in persons born between 1935 and 1959 suggesting a past period of increased infection pressure. Paired samples of individuals participating in 1998 and 2010 (n = 2564) revealed respective seroconversion and seroreversion rates of 6.2% and 22.6% among seronegative and seropositive individuals during 12 years, or 5.2 and 2.9 per 1000 inhabitants per year. This corresponds to a total of 417 242 [95%CI: 344 363-495 971] new seroconversions per year in the German population. While anti-HEV seroprevalence has decreased in the last decade, infection pressure and seroincidence remains high in Germany. Continuously rising numbers of Hepatitis E diagnoses in Europe are likely due to an increased awareness of clinicians and indicate that still there is a gap between incident and diagnosed cases. Studies on the true burden of the disease, specific risk factors and sources of autochthonous infections as well as targeted prevention measures are urgently needed. CI - © 2018 John Wiley & Sons Ltd. FAU - Faber, M AU - Faber M AUID- ORCID: 0000-0002-9196-570X AD - Department for Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany. FAU - Willrich, N AU - Willrich N AD - Department for Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany. FAU - Schemmerer, M AU - Schemmerer M AD - Institute of Clinical Microbiology and Hygiene, University Medical Center Regensburg, Regensburg, Germany. FAU - Rauh, C AU - Rauh C AD - Institute of Clinical Microbiology and Hygiene, University Medical Center Regensburg, Regensburg, Germany. FAU - Kuhnert, R AU - Kuhnert R AD - Department of Epidemiology and Health Reporting, Robert Koch Institute, Berlin, Germany. FAU - Stark, K AU - Stark K AD - Department for Infectious Disease Epidemiology, Robert Koch Institute, Berlin, Germany. FAU - Wenzel, J J AU - Wenzel JJ AUID- ORCID: 0000-0001-8422-6581 AD - Institute of Clinical Microbiology and Hygiene, University Medical Center Regensburg, Regensburg, Germany. LA - eng PT - Journal Article DEP - 20180301 PL - England TA - J Viral Hepat JT - Journal of viral hepatitis JID - 9435672 RN - 0 (Hepatitis Antibodies) RN - 0 (Immunoglobulin G) SB - IM MH - Adolescent MH - Adult MH - Aged MH - Betacoronavirus 1 MH - Female MH - Germany/epidemiology MH - Hepatitis Antibodies/blood MH - Hepatitis E/*epidemiology MH - Hepatitis E virus/*immunology MH - Humans MH - Immunoglobulin G/blood MH - Incidence MH - Male MH - Middle Aged MH - Seroconversion MH - Seroepidemiologic Studies MH - Young Adult OTO - NOTNLM OT - anti-HEV IgG OT - epidemiology OT - foodborne infections OT - hepatitis E OT - longitudinal study OT - viral hepatitis EDAT- 2018/01/30 06:00 MHDA- 2018/10/16 06:00 CRDT- 2018/01/30 06:00 PHST- 2017/09/04 00:00 [received] PHST- 2017/12/18 00:00 [accepted] PHST- 2018/01/30 06:00 [pubmed] PHST- 2018/10/16 06:00 [medline] PHST- 2018/01/30 06:00 [entrez] AID - 10.1111/jvh.12868 [doi] PST - ppublish SO - J Viral Hepat. 2018 Jun;25(6):752-758. doi: 10.1111/jvh.12868. Epub 2018 Mar 1. PMID- 29875237 OWN - NLM STAT- MEDLINE DCOM- 20180817 LR - 20190131 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 16 DP - 2018 Aug 15 TI - Ulk1 Governs Nerve Growth Factor/TrkA Signaling by Mediating Rab5 GTPase Activation in Porcine Hemagglutinating Encephalomyelitis Virus-Induced Neurodegenerative Disorders. LID - 10.1128/JVI.00325-18 [doi] LID - e00325-18 AB - Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus and causes neurological dysfunction in the central nervous system (CNS), but the neuropathological mechanism of PHEV remains poorly understood. We report that Unc51-like kinase 1 (Ulk1/Unc51.1) is a pivotal regulator of PHEV-induced neurological disorders and functions to selectively control the initiation of nerve growth factor (NGF)/TrkA endosome trafficking. We first identified the function of Ulk1 by histopathologic evaluation in a PHEV-infected mouse model in which neuronal loss was accompanied by the suppression of Ulk1 expression. Morphogenesis assessments in the primary cortical neurons revealed that overexpression or mutations of Ulk1 modulated neurite outgrowth, collateral sprouting, and endosomal transport. Likewise, Ulk1 expression was decreased following PHEV infection, suggesting that there was a correlation between the neurodegeneration and functional Ulk1 deficiency. We then showed that Ulk1 forms a multiprotein complex with TrkA and the early endosome marker Rab5 and that Ulk1 defects lead to either blocking of NGF/TrkA endocytosis or premature degradation of pTrkA via constitutive activation of the Rab5 GTPase. Further investigation determined that the ectopic expression of Rab5 mutants induces aberrant endosomal accumulation of activated pTrkA, proving that targeting of Ulk1-TrkA-NGF signaling to the retrograde transport route in the neurodegenerative process that underlies PHEV infection is dependent on Rab5 GTPase activity. Therefore, we described a long-distance signaling mechanism of PHEV-driven deficits in neurons and suggested that such Ulk1 repression may result in limited NGF/TrkA retrograde signaling within activated Rab5 endosomes, explaining the progressive failure of neurite outgrowth and survival.IMPORTANCE Porcine hemagglutinating encephalomyelitis virus (PHEV) is a neurotropic coronavirus and targets neurons in the nervous system for proliferation, frequently leaving behind grievous neurodegeneration. Structural plasticity disorders occur in the axons, dendrites, and dendritic spines of PHEV-infected neurons, and dysfunction of this neural process may contribute to neurologic pathologies, but the mechanisms remain undetermined. Further understanding of the neurological manifestations underlying PHEV infection in the CNS may provide insights into both neurodevelopmental and neurodegenerative diseases that may be conducive to targeted approaches for treatment. The significance of our research is in identifying an Ulk1-related neurodegenerative mechanism, focusing on the regulatory functions of Ulk1 in the transport of long-distance trophic signaling endosomes, thereby explaining the progressive failure of neurite outgrowth and survival associated with PHEV aggression. This is the first report to define a mechanistic link between alterations in signaling from endocytic pathways and the neuropathogenesis of PHEV-induced CNS disease. CI - Copyright © 2018 American Society for Microbiology. FAU - Li, Zi AU - Li Z AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Zhao, Kui AU - Zhao K AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lv, Xiaoling AU - Lv X AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lan, Yungang AU - Lan Y AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Hu, Shiyu AU - Hu S AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Shi, Junchao AU - Shi J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Guan, Jiyu AU - Guan J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Yang, Yawen AU - Yang Y AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lu, Huijun AU - Lu H AD - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China. FAU - He, Hongbin AU - He H AD - Key Laboratory of Animal Resistant Biology of Shandong, Ruminant Disease Research Center, College of Life Sciences, Shandong Normal University, Jinan, China. FAU - Gao, Feng AU - Gao F AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - He, Wenqi AU - He W AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China hewq@jlu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180731 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 9061-61-4 (Nerve Growth Factor) RN - EC 2.7.10.1 (Receptor, trkA) RN - EC 2.7.11.1 (Autophagy-Related Protein-1 Homolog) RN - EC 2.7.11.1 (Ulk1 protein, mouse) RN - EC 3.6.5.2 (rab5 GTP-Binding Proteins) SB - IM MH - Animals MH - Autophagy-Related Protein-1 Homolog/*metabolism MH - Betacoronavirus 1/*growth & development MH - Coronavirus Infections/pathology/*veterinary MH - Disease Models, Animal MH - Host-Pathogen Interactions MH - Mice MH - Nerve Growth Factor/*metabolism MH - Neurodegenerative Diseases/pathology/*veterinary MH - Receptor, trkA/*metabolism MH - Signal Transduction MH - rab5 GTP-Binding Proteins/*metabolism PMC - PMC6069187 OTO - NOTNLM OT - NGF OT - Rab5 OT - Ulk1 OT - neurodegeneration OT - neurovirulent coronavirus OT - porcine hemagglutinating encephalomyelitis virus EDAT- 2018/06/08 06:00 MHDA- 2018/08/18 06:00 PMCR- 2019/01/31 CRDT- 2018/06/08 06:00 PHST- 2018/02/27 00:00 [received] PHST- 2018/05/25 00:00 [accepted] PHST- 2018/06/08 06:00 [pubmed] PHST- 2018/08/18 06:00 [medline] PHST- 2018/06/08 06:00 [entrez] PHST- 2019/01/31 00:00 [pmc-release] AID - JVI.00325-18 [pii] AID - 00325-18 [pii] AID - 10.1128/JVI.00325-18 [doi] PST - epublish SO - J Virol. 2018 Jul 31;92(16):e00325-18. doi: 10.1128/JVI.00325-18. Print 2018 Aug 15. PMID- 29800601 OWN - NLM STAT- MEDLINE DCOM- 20181023 LR - 20200407 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 253 DP - 2018 Jul 15 TI - Porcine epidemic diarrhea virus through p53-dependent pathway causes cell cycle arrest in the G0/G1 phase. PG - 1-11 LID - S0168-1702(18)30175-8 [pii] LID - 10.1016/j.virusres.2018.05.019 [doi] AB - Porcine epidemic diarrhea virus (PEDV), an enteropathogenic Alphacoronavirus, has caused enormous economic losses in the swine industry. p53 protein exists in a wide variety of animal cells, which is involved in cell cycle regulation, apoptosis, cell differentiation and other biological functions. In this study, we investigated the effects of PEDV infection on the cell cycle of Vero cells and p53 activation. The results demonstrated that PEDV infection induces cell cycle arrest at G0/G1 phase in Vero cells, while UV-inactivated PEDV does not cause cell cycle arrest. PEDV infection up-regulates the levels of p21, cdc2, cdk2, cdk4, Cyclin A protein and down-regulates Cyclin E protein. Further research results showed that inhibition of p53 signaling pathway can reverse the cell cycle arrest in G0/G1 phase induced by PEDV infection and cancel out the up-regulation of p21 and corresponding Cyclin/cdk mentioned above. In addition, PEDV infection of the cells synchronized in various stages of cell cycle showed that viral subgenomic RNA and virus titer were higher in the cells released from G0/G1 phase synchronized cells than that in the cells released from the G1/S phase and G2/M phase synchronized or asynchronous cells after 18 h p.i.. This is the first report to demonstrate that the p53-dependent pathway plays an important role in PEDV induced cell cycle arrest and beneficially contributes to viral infection. CI - Copyright © 2018 Elsevier B.V. All rights reserved. FAU - Sun, Pei AU - Sun P AD - College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, 230036, China. FAU - Wu, Haoyang AU - Wu H AD - College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, 230036, China. FAU - Huang, Jiali AU - Huang J AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. FAU - Xu, Ying AU - Xu Y AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. FAU - Yang, Feng AU - Yang F AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. FAU - Zhang, Qi AU - Zhang Q AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. FAU - Xu, Xingang AU - Xu X AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address: tiger2003@nwsuaf.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180522 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Cyclin-Dependent Kinase Inhibitor p21) RN - 0 (Tumor Suppressor Protein p53) RN - EC 2.7.11.22 (Cyclin-Dependent Kinase 2) SB - IM MH - Animals MH - Chlorocebus aethiops MH - Coronavirus Infections/metabolism/physiopathology/*veterinary/virology MH - Cyclin-Dependent Kinase 2/genetics/metabolism MH - Cyclin-Dependent Kinase Inhibitor p21/genetics/metabolism MH - G1 Phase MH - *G1 Phase Cell Cycle Checkpoints MH - Porcine epidemic diarrhea virus MH - Resting Phase, Cell Cycle MH - Swine MH - Swine Diseases/genetics/*metabolism/physiopathology/virology MH - Tumor Suppressor Protein p53/genetics/*metabolism MH - Vero Cells PMC - PMC7114671 OTO - NOTNLM OT - Cell cycle arrest OT - PEDV OT - Virus replication OT - p53 pathway EDAT- 2018/05/26 06:00 MHDA- 2018/10/24 06:00 PMCR- 2018/05/22 CRDT- 2018/05/26 06:00 PHST- 2018/03/17 00:00 [received] PHST- 2018/05/17 00:00 [revised] PHST- 2018/05/21 00:00 [accepted] PHST- 2018/05/26 06:00 [pubmed] PHST- 2018/10/24 06:00 [medline] PHST- 2018/05/26 06:00 [entrez] PHST- 2018/05/22 00:00 [pmc-release] AID - S0168-1702(18)30175-8 [pii] AID - 10.1016/j.virusres.2018.05.019 [doi] PST - ppublish SO - Virus Res. 2018 Jul 15;253:1-11. doi: 10.1016/j.virusres.2018.05.019. Epub 2018 May 22. PMID- 25428188 OWN - NLM STAT- MEDLINE DCOM- 20150914 LR - 20190522 IS - 1943-4936 (Electronic) IS - 1040-6387 (Linking) VI - 27 IP - 1 DP - 2015 Jan TI - Enteric disease in postweaned beef calves associated with Bovine coronavirus clade 2. PG - 97-101 LID - 10.1177/1040638714559026 [doi] AB - Bovine coronavirus (BoCV; Betacoronavirus 1) infections are associated with varied clinical presentations including neonatal diarrhea, winter dysentery in dairy cattle, and respiratory disease in various ages of cattle. The current report presents information on BoCV infections associated with enteric disease of postweaned beef cattle in Oklahoma. In 3 separate accessions from a single herd, 1 in 2012 and 2 in 2013, calves were observed with bloody diarrhea. One calf in 2012 died and was necropsied, and 2 calves from this herd died in 2013 and were necropsied. A third calf from another herd died and was necropsied. The gross and histologic diagnosis was acute, hemorrhagic colitis in all 4 cattle. Colonic tissues from all 4 animals were positive by fluorescent antibody testing and/or immunohistochemical staining for BoCV antigen. Bovine coronavirus was isolated in human rectal tumor cells from swabs of colon surfaces of all animals. The genomic information from a region of the S envelope region revealed BoCV clade 2. Detection of BoCV clade 2 in beef cattle in Oklahoma is consistent with recovery of BoCV clade 2 from the respiratory tract of postweaned beef calves that had respiratory disease signs or were healthy. Further investigations on the ecology of BoCV in cattle are important, as BoCV may be an emerging disease beyond the initial descriptions. Challenge studies are needed to determine pathogenicity of these strains, and to determine if current BoCV vaccines are efficacious against the BoCV clade 2 strains. CI - © 2014 The Author(s). FAU - Fulton, Robert W AU - Fulton RW AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK (Fulton, Herd, Sorensen, Confer, Ritchey, Burge)U.S. Department of Agriculture, Agriculture Research Service, National Animal Disease Center, Ames, IA (Ridpath) robert.fulton@okstate.edu. FAU - Herd, Heather R AU - Herd HR AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK (Fulton, Herd, Sorensen, Confer, Ritchey, Burge)U.S. Department of Agriculture, Agriculture Research Service, National Animal Disease Center, Ames, IA (Ridpath). FAU - Sorensen, Nicholas J AU - Sorensen NJ AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK (Fulton, Herd, Sorensen, Confer, Ritchey, Burge)U.S. Department of Agriculture, Agriculture Research Service, National Animal Disease Center, Ames, IA (Ridpath). FAU - Confer, Anthony W AU - Confer AW AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK (Fulton, Herd, Sorensen, Confer, Ritchey, Burge)U.S. Department of Agriculture, Agriculture Research Service, National Animal Disease Center, Ames, IA (Ridpath). FAU - Ritchey, Jerry W AU - Ritchey JW AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK (Fulton, Herd, Sorensen, Confer, Ritchey, Burge)U.S. Department of Agriculture, Agriculture Research Service, National Animal Disease Center, Ames, IA (Ridpath). FAU - Ridpath, Julia F AU - Ridpath JF AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK (Fulton, Herd, Sorensen, Confer, Ritchey, Burge)U.S. Department of Agriculture, Agriculture Research Service, National Animal Disease Center, Ames, IA (Ridpath). FAU - Burge, Lurinda J AU - Burge LJ AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK (Fulton, Herd, Sorensen, Confer, Ritchey, Burge)U.S. Department of Agriculture, Agriculture Research Service, National Animal Disease Center, Ames, IA (Ridpath). LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20141126 PL - United States TA - J Vet Diagn Invest JT - Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc JID - 9011490 SB - IM MH - Animals MH - Cattle MH - Cattle Diseases/*diagnosis/microbiology/pathology MH - Colitis/diagnosis/microbiology/pathology/*veterinary MH - Coronavirus Infections/diagnosis/microbiology/pathology/*veterinary MH - Coronavirus, Bovine/*isolation & purification MH - Female MH - Male MH - Molecular Sequence Data MH - Oklahoma MH - Phylogeny MH - Sequence Analysis, DNA/veterinary OTO - NOTNLM OT - Beef cattle OT - Bovine coronavirus OT - enteric disease EDAT- 2014/11/28 06:00 MHDA- 2015/09/15 06:00 CRDT- 2014/11/28 06:00 PHST- 2014/11/28 06:00 [entrez] PHST- 2014/11/28 06:00 [pubmed] PHST- 2015/09/15 06:00 [medline] AID - 1040638714559026 [pii] AID - 10.1177/1040638714559026 [doi] PST - ppublish SO - J Vet Diagn Invest. 2015 Jan;27(1):97-101. doi: 10.1177/1040638714559026. Epub 2014 Nov 26. PMID- 25589656 OWN - NLM STAT- MEDLINE DCOM- 20150521 LR - 20201209 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 7 DP - 2015 Apr TI - The nsp1, nsp13, and M proteins contribute to the hepatotropism of murine coronavirus JHM.WU. PG - 3598-609 LID - 10.1128/JVI.03535-14 [doi] AB - Mouse hepatitis virus (MHV) isolates JHM.WU and JHM.SD promote severe central nervous system disease. However, while JHM.WU replicates robustly and induces hepatitis, JHM.SD fails to replicate or induce pathology in the liver. These two JHM variants encode homologous proteins with few polymorphisms, and little is known about which viral proteins(s) is responsible for the liver tropism of JHM.WU. We constructed reverse genetic systems for JHM.SD and JHM.WU and, utilizing these full-length cDNA clones, constructed chimeric viruses and mapped the virulence factors involved in liver tropism. Exchanging the spike proteins of the two viruses neither increased replication of JHM.SD in the liver nor attenuated JHM.WU. By further mapping, we found that polymorphisms in JHM.WU structural protein M and nonstructural replicase proteins nsp1 and nsp13 are essential for liver pathogenesis. M protein and nsp13, the helicase, of JHM.WU are required for efficient replication in vitro and in the liver in vivo. The JHM.SD nsp1 protein contains a K194R substitution of Lys194, a residue conserved among all other MHV strains. The K194R polymorphism has no effect on in vitro replication but influences hepatotropism, and introduction of R194K into JHM.SD promotes replication in the liver. Conversely, a K194R substitution in nsp1 of JHM.WU or A59, another hepatotropic strain, significantly attenuates replication of each strain in the liver and increases IFN-β expression in macrophages in culture. Our data indicate that both structural and nonstructural proteins contribute to MHV liver pathogenesis and support previous reports that nsp1 is a Betacoronavirus virulence factor. IMPORTANCE: The Betacoronavirus genus includes human pathogens, some of which cause severe respiratory disease. The spread of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) into human populations demonstrates the zoonotic potential of emerging coronaviruses, and there are currently no vaccines or effective antivirals for human coronaviruses. Thus, it is important to understand the virus-host interaction that regulates coronavirus pathogenesis. Murine coronavirus infection of mice provides a useful model for the study of coronavirus-host interactions, including the determinants of tropism and virulence. We found that very small changes in coronavirus proteins can profoundly affect tropism and virulence. Furthermore, the hepatotropism of MHV-JHM depends not on the spike protein and viral entry but rather on a combination of the structural protein M and nonstructural replicase-associated proteins nsp1 and nsp13, which are conserved among betacoronaviruses. Understanding virulence determinants will aid in the design of vaccines and antiviral strategies. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Zhang, Rong AU - Zhang R AD - Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Li, Yize AU - Li Y AD - Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Cowley, Timothy J AU - Cowley TJ AD - Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Steinbrenner, Adam D AU - Steinbrenner AD AD - Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Phillips, Judith M AU - Phillips JM AD - Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Yount, Boyd L AU - Yount BL AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Baric, Ralph S AU - Baric RS AD - Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Weiss, Susan R AU - Weiss SR AUID- ORCID: 0000-0002-8155-4528 AD - Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA weisssr@upenn.edu. LA - eng GR - K08-AI098503/AI/NIAID NIH HHS/United States GR - T32 AI007324/AI/NIAID NIH HHS/United States GR - U19 AI109761/AI/NIAID NIH HHS/United States GR - R01-NS081008/NS/NINDS NIH HHS/United States GR - T32-AI007324/AI/NIAID NIH HHS/United States GR - R01 NS081008/NS/NINDS NIH HHS/United States GR - K08 AI098503/AI/NIAID NIH HHS/United States GR - R56 AI095285/AI/NIAID NIH HHS/United States GR - R56-AI095285/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20150114 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Coronavirus M Proteins) RN - 0 (Viral Matrix Proteins) RN - 0 (Viral Nonstructural Proteins) SB - IM MH - Animals MH - Cell Line MH - Coronavirus M Proteins MH - Cricetinae MH - Hepatitis, Viral, Animal/virology MH - Liver/*virology MH - Mice, Inbred C57BL MH - Murine hepatitis virus/genetics/*physiology MH - Reverse Genetics MH - Viral Matrix Proteins/genetics/*metabolism MH - Viral Nonstructural Proteins/genetics/*metabolism MH - *Viral Tropism MH - Virus Replication PMC - PMC4403414 EDAT- 2015/01/16 06:00 MHDA- 2015/05/23 06:00 PMCR- 2015/10/01 CRDT- 2015/01/16 06:00 PHST- 2015/01/16 06:00 [entrez] PHST- 2015/01/16 06:00 [pubmed] PHST- 2015/05/23 06:00 [medline] PHST- 2015/10/01 00:00 [pmc-release] AID - JVI.03535-14 [pii] AID - 03535-14 [pii] AID - 10.1128/JVI.03535-14 [doi] PST - ppublish SO - J Virol. 2015 Apr;89(7):3598-609. doi: 10.1128/JVI.03535-14. Epub 2015 Jan 14. PMID- 28352124 OWN - NLM STAT- MEDLINE DCOM- 20170406 LR - 20181113 IS - 2222-1751 (Electronic) IS - 2222-1751 (Linking) VI - 6 IP - 3 DP - 2017 Mar 29 TI - Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China. PG - e14 LID - 10.1038/emi.2016.140 [doi] AB - To investigate bat coronaviruses (CoVs), we collected 132 rectal swabs and urine samples from five bat species in three countries in southwestern China. Seven CoVs belonging to distinct groups of severe acute respiratory syndrome (SARS)-like CoVs and α-CoVs were detected in samples from least horseshoe bats. Samples from other bat species were negative for these viruses, indicating that the least horseshoe bat represents one of the natural reservoirs and mixers for strains of CoVs and has a pivotal role in the evolution and dissemination of these viruses. The genetic and evolutionary characteristics of these strains were described. Whole-genome sequencing of a new isolate (F46) from a rectal swab from a least horseshoe bat showed that it contained 29 699 nucleotides, excluding the poly (A) tail, with 13 open reading frames (ORFs). Phylogenetic and recombination analyses of F46 provided evidence of natural recombination between bat SARS-like CoVs (Rs3367 and LYRa11) or SARS-CoV (BJ01), suggesting that F46 could be a new recombinant virus from SARS-like CoVs or SARS-CoVs. FAU - Wang, Lihua AU - Wang L AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China. FAU - Fu, Shihong AU - Fu S AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China. FAU - Cao, Yuxi AU - Cao Y AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China. FAU - Zhang, Hailin AU - Zhang H AD - Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China. FAU - Feng, Yun AU - Feng Y AD - Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China. FAU - Yang, Weihong AU - Yang W AD - Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China. FAU - Nie, Kai AU - Nie K AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China. FAU - Ma, Xuejun AU - Ma X AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China. FAU - Liang, Guodong AU - Liang G AD - State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China. LA - eng PT - Journal Article DEP - 20170329 PL - United States TA - Emerg Microbes Infect JT - Emerging microbes & infections JID - 101594885 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Chiroptera/classification/*virology MH - Coronavirus/*classification/genetics/*isolation & purification MH - Evolution, Molecular MH - Genome Size MH - *Genome, Viral MH - Open Reading Frames MH - Phylogeny MH - RNA, Viral/genetics MH - Recombination, Genetic MH - Rectum/virology MH - Urine/virology PMC - PMC5378919 EDAT- 2017/03/30 06:00 MHDA- 2017/04/07 06:00 PMCR- 2017/03/01 CRDT- 2017/03/30 06:00 PHST- 2016/08/23 00:00 [received] PHST- 2016/12/21 00:00 [revised] PHST- 2016/12/27 00:00 [accepted] PHST- 2017/03/30 06:00 [entrez] PHST- 2017/03/30 06:00 [pubmed] PHST- 2017/04/07 06:00 [medline] PHST- 2017/03/01 00:00 [pmc-release] AID - emi2016140 [pii] AID - 10.1038/emi.2016.140 [doi] PST - epublish SO - Emerg Microbes Infect. 2017 Mar 29;6(3):e14. doi: 10.1038/emi.2016.140. PMID- 28623921 OWN - NLM STAT- MEDLINE DCOM- 20180308 LR - 20181113 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 14 IP - 1 DP - 2017 Jun 17 TI - Genome-wide analysis of codon usage bias in Bovine Coronavirus. PG - 115 LID - 10.1186/s12985-017-0780-y [doi] LID - 115 AB - BACKGROUND: Bovine coronavirus (BCoV) belong to the genus Betacoronavirus of the family Coronaviridae. BCoV are widespread around the world and cause enteric or respiratory infections among cattle, leading to important economic losses to the beef and dairy industry worldwide. To study the relation of codon usage among viruses and their hosts is essential to understand host-pathogen interaction, evasion from host's immune system and evolution. METHODS: We performed a comprehensive analysis of codon usage and composition of BCoV. RESULTS: The global codon usage among BCoV strains is similar. Significant differences of codon preferences in BCoV genes in relation to codon usage of Bos taurus host genes were found. Most of the highly frequent codons are U-ending. G + C compositional constraint and dinucleotide composition also plays a role in the overall pattern of BCoV codon usage. CONCLUSIONS: The results of these studies revealed that mutational bias is a leading force shaping codon usage in this virus. Additionally, relative dinucleotide frequencies, geographical distribution, and evolutionary processes also influenced the codon usage pattern. FAU - Castells, Matías AU - Castells M AD - Laboratorio de Virología Molecular, Sede Salto, Centro Universitario Regional Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay. FAU - Victoria, Matías AU - Victoria M AD - Laboratorio de Virología Molecular, Sede Salto, Centro Universitario Regional Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay. FAU - Colina, Rodney AU - Colina R AD - Laboratorio de Virología Molecular, Sede Salto, Centro Universitario Regional Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay. FAU - Musto, Héctor AU - Musto H AD - Laboratorio de Organización y Evolución del Genoma, Unidad de Genómica Evolutiva, Instituto de Biología, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay. FAU - Cristina, Juan AU - Cristina J AD - Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay. cristina@cin.edu.uy. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170617 PL - England TA - Virol J JT - Virology journal JID - 101231645 RN - 0 (Codon) SB - IM MH - Adaptation, Biological MH - Animals MH - Cattle MH - *Codon MH - Coronavirus, Bovine/*genetics MH - Evolution, Molecular MH - *Genome, Viral MH - *Protein Biosynthesis PMC - PMC5474002 OTO - NOTNLM OT - Bovine OT - Codon usage OT - Coronavirus OT - Evolution EDAT- 2017/06/19 06:00 MHDA- 2018/03/09 06:00 PMCR- 2017/06/17 CRDT- 2017/06/19 06:00 PHST- 2017/01/26 00:00 [received] PHST- 2017/06/06 00:00 [accepted] PHST- 2017/06/19 06:00 [entrez] PHST- 2017/06/19 06:00 [pubmed] PHST- 2018/03/09 06:00 [medline] PHST- 2017/06/17 00:00 [pmc-release] AID - 10.1186/s12985-017-0780-y [pii] AID - 780 [pii] AID - 10.1186/s12985-017-0780-y [doi] PST - epublish SO - Virol J. 2017 Jun 17;14(1):115. doi: 10.1186/s12985-017-0780-y. PMID- 30353949 OWN - NLM STAT- MEDLINE DCOM- 20190117 LR - 20210109 IS - 1939-1676 (Electronic) IS - 0891-6640 (Print) IS - 0891-6640 (Linking) VI - 32 IP - 6 DP - 2018 Nov TI - Investigation of an experimental infection model of equine coronavirus in adult horses. PG - 2099-2104 LID - 10.1111/jvim.15318 [doi] AB - BACKGROUND: Equine coronavirus (ECoV) is a recently reported enteric disease of adult horses. Natural infection by ECoV has been reported in adult horses worldwide, whereas experimental infection has only been reported in juvenile horses. An experimental infection model is needed to study the clinical presentation, laboratory abnormalities, and pathophysiological changes associated with ECoV. OBJECTIVES: To investigate the clinical, hematologic, molecular, and serological features of adult horses experimentally infected with ECoV. ANIMALS: Eight adult horses. METHODS: Four horses were intragastrically infected with fecal material containing 10(9) genome equivalents of ECoV. Four additional horses were exposed daily to the feces from the experimentally-infected horses. Monitoring included physical examinations, as well as daily nasal swab, whole blood, and fecal collection for molecular detection of ECoV. Blood was collected every other day for hematologic analysis and weekly for serologic analysis. RESULTS: All 8 horses shed ECoV in feces. Six of the 8 horses (75%) exhibited mild, clinical disease with soft, formed manure; 1 horse exhibited transient pyrexia. All horses maintained total white cell counts within normal limits, but 3 horses developed transient lymphopenia. No statistically significant differences (P = .20) were observed in quantity of fecal shedding of ECoV between the 2 groups. CONCLUSIONS AND CLINICAL IMPORTANCE: Experimental infection of adult horses with ECoV was associated with mild and self-limiting clinical signs, transient lymphopenia, and fecal shedding of ECoV, which mimics natural infection. No differences between experimentally-infected horses and horses exposed to ECoV-containing feces were identified. Results of our study support a fecal-oral route of transmission. CI - © 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine. FAU - Schaefer, Emily AU - Schaefer E AUID- ORCID: 0000-0002-8314-3861 AD - William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, Davis, California. FAU - Harms, Corey AU - Harms C AD - William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, Davis, California. FAU - Viner, Molly AU - Viner M AD - William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, Davis, California. FAU - Barnum, Samantha AU - Barnum S AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, California. FAU - Pusterla, Nicola AU - Pusterla N AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, California. LA - eng GR - Boehringer Ingelheim/ PT - Journal Article DEP - 20181024 PL - United States TA - J Vet Intern Med JT - Journal of veterinary internal medicine JID - 8708660 SB - IM MH - Animals MH - *Betacoronavirus 1 MH - Coronavirus Infections/pathology/transmission/*veterinary/virology MH - Disease Models, Animal MH - Feces/virology MH - Female MH - Horse Diseases/pathology/transmission/*virology MH - Horses MH - Lymphocyte Count/veterinary MH - Male MH - Real-Time Polymerase Chain Reaction/veterinary MH - Virus Shedding PMC - PMC6271284 OTO - NOTNLM OT - enteric OT - experimental infection OT - polymerase chain reaction OT - serology EDAT- 2018/10/26 06:00 MHDA- 2019/01/18 06:00 PMCR- 2018/11/01 CRDT- 2018/10/25 06:00 PHST- 2017/11/14 00:00 [received] PHST- 2018/06/02 00:00 [revised] PHST- 2018/08/09 00:00 [accepted] PHST- 2018/10/26 06:00 [pubmed] PHST- 2019/01/18 06:00 [medline] PHST- 2018/10/25 06:00 [entrez] PHST- 2018/11/01 00:00 [pmc-release] AID - JVIM15318 [pii] AID - 10.1111/jvim.15318 [doi] PST - ppublish SO - J Vet Intern Med. 2018 Nov;32(6):2099-2104. doi: 10.1111/jvim.15318. Epub 2018 Oct 24. PMID- 29355607 OWN - NLM STAT- MEDLINE DCOM- 20190110 LR - 20231113 IS - 1567-7257 (Electronic) IS - 1567-1348 (Print) IS - 1567-1348 (Linking) VI - 58 DP - 2018 Mar TI - Interplay between co-divergence and cross-species transmission in the evolutionary history of bat coronaviruses. PG - 279-289 LID - S1567-1348(18)30012-1 [pii] LID - 10.1016/j.meegid.2018.01.012 [doi] AB - Coronaviruses (CoVs) have been documented in almost every species of bat sampled. Bat CoVs exhibit both extensive genetic diversity and a broad geographic range, indicative of a long-standing host association. Despite this, the respective roles of long-term virus-host co-divergence and cross-species transmission (host-jumping) in the evolution of bat coronaviruses are unclear. Using a phylogenetic approach we provide evidence that CoV diversity in bats is shaped by both species richness and their geographical distribution, and that CoVs exhibit clustering at the level of bat genera, with these genus-specific clusters largely associated with distinct CoV species. Co-phylogenetic analyses revealed that cross-species transmission has been more common than co-divergence across coronavirus evolution as a whole, and that cross-species transmission events were more likely between sympatric bat hosts. Notably, however, an analysis of the CoV RNA polymerase phylogeny suggested that many such host-jumps likely resulted in short-term spill-over infections, with little evidence for sustained onward transmission in new co-roosting host species. CI - Copyright © 2018 Elsevier B.V. All rights reserved. FAU - Leopardi, Stefania AU - Leopardi S AD - National Reference Centre, OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita' 10, Legnaro, Padova 35020, Italy. Electronic address: Sleopardi@izsvenezie.it. FAU - Holmes, Edward C AU - Holmes EC AD - Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, Australia. FAU - Gastaldelli, Michele AU - Gastaldelli M AD - National Reference Centre, OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita' 10, Legnaro, Padova 35020, Italy. FAU - Tassoni, Luca AU - Tassoni L AD - National Reference Centre, OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita' 10, Legnaro, Padova 35020, Italy. FAU - Priori, Pamela AU - Priori P AD - S.T.E.R.N.A., Forlì, Italy. FAU - Scaravelli, Dino AU - Scaravelli D AD - S.T.E.R.N.A., Forlì, Italy. FAU - Zamperin, Gianpiero AU - Zamperin G AD - National Reference Centre, OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita' 10, Legnaro, Padova 35020, Italy. FAU - De Benedictis, Paola AU - De Benedictis P AD - National Reference Centre, OIE Collaborating Centre for Diseases at the Animal-Human Interface, Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Universita' 10, Legnaro, Padova 35020, Italy. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180130 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 SB - IM MH - Animal Diseases/*transmission/*virology MH - Animals MH - Chiroptera/*virology MH - Coronavirus/classification/*genetics MH - Coronavirus Infections/*veterinary MH - Evolution, Molecular MH - Genetic Variation MH - Genome, Viral MH - Host Specificity MH - Phylogeny MH - Phylogeography PMC - PMC7106311 OTO - NOTNLM OT - Bats OT - Coronaviruses OT - Cross-species transmission OT - Evolution OT - Phylogeny co-divergence OT - Virus EDAT- 2018/01/23 06:00 MHDA- 2019/01/11 06:00 PMCR- 2018/01/30 CRDT- 2018/01/23 06:00 PHST- 2017/09/25 00:00 [received] PHST- 2018/01/10 00:00 [revised] PHST- 2018/01/12 00:00 [accepted] PHST- 2018/01/23 06:00 [pubmed] PHST- 2019/01/11 06:00 [medline] PHST- 2018/01/23 06:00 [entrez] PHST- 2018/01/30 00:00 [pmc-release] AID - S1567-1348(18)30012-1 [pii] AID - 10.1016/j.meegid.2018.01.012 [doi] PST - ppublish SO - Infect Genet Evol. 2018 Mar;58:279-289. doi: 10.1016/j.meegid.2018.01.012. Epub 2018 Jan 30. PMID- 30198895 OWN - NLM STAT- MEDLINE DCOM- 20181106 LR - 20241212 IS - 2059-7983 (Electronic) IS - 2059-7983 (Linking) VI - 74 IP - Pt 9 DP - 2018 Sep 1 TI - Crystal structure of the post-fusion core of the Human coronavirus 229E spike protein at 1.86 Å resolution. PG - 841-851 LID - 10.1107/S2059798318008318 [doi] AB - Human coronavirus 229E (HCoV-229E) usually causes mild upper respiratory infections in heathy adults, but may lead to severe complications or mortality in individuals with weakened immune systems. Virus entry of HCoV-229E is mediated by its spike (S) protein, where the S1 domain facilitates attachment to host cells and the S2 domain is involved in subsequent fusion of the virus and host membranes. During the fusion process, two heptad repeats, HR1 and HR2, in the S2 domain assemble into a six-helix membrane-fusion structure termed the fusion core. Here, the complete fusion-core structure of HCoV-229E has been determined at 1.86 Å resolution, representing the most complete post-fusion conformation thus far among published human alphacoronavirus (α-HCoV) fusion-core structures. The overall structure of the HCoV-229E fusion core is similar to those of SARS, MERS and HCoV-NL63, but the packing of its 3HR1 core differs from those of SARS and MERS in that it contains more noncanonical `x' and `da' layers. Side-by-side electrostatic surface comparisons reveal that the electrostatic surface potentials are opposite in α-HCoVs and β-HCoVs at certain positions and that the HCoV-229E surface also appears to be the most hydrophobic among the various HCoVs. In addition to the highly conserved hydrophobic interactions between HR1 and HR2, some polar and electrostatic interactions are also well preserved across different HCoVs. This study adds to the structural profiling of HCoVs to aid in the structure-based design of pan-coronavirus small molecules or peptides to inhibit viral fusion. CI - open access. FAU - Yan, Lei AU - Yan L AD - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai 201210, People's Republic of China. FAU - Meng, Bing AU - Meng B AD - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai 201210, People's Republic of China. FAU - Xiang, Jiangchao AU - Xiang J AD - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai 201210, People's Republic of China. FAU - Wilson, Ian A AU - Wilson IA AD - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai 201210, People's Republic of China. FAU - Yang, Bei AU - Yang B AUID- ORCID: 0000-0001-5389-3859 AD - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 393 Middle Huaxia Road, Shanghai 201210, People's Republic of China. LA - eng GR - 31600619/National Natural Science Foundation of China/ GR - 16PJ1407500/Science and Technology Commission of Shanghai Municipality/ PT - Journal Article DEP - 20180903 PL - United States TA - Acta Crystallogr D Struct Biol JT - Acta crystallographica. Section D, Structural biology JID - 101676043 RN - 0 (Recombinant Fusion Proteins) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Amino Acid Sequence MH - Coronavirus 229E, Human/*isolation & purification/physiology MH - Coronavirus Infections/*virology MH - Crystallography, X-Ray MH - Humans MH - Models, Molecular MH - Protein Conformation MH - Recombinant Fusion Proteins/*chemistry MH - Sequence Alignment MH - Spike Glycoprotein, Coronavirus/*chemistry PMC - PMC6130466 OTO - NOTNLM OT - HCoV-229E OT - MERS OT - SARS OT - X-ray structure OT - coronavirus OT - post-fusion core OT - spike protein EDAT- 2018/09/11 06:00 MHDA- 2018/11/07 06:00 PMCR- 2018/09/03 CRDT- 2018/09/11 06:00 PHST- 2018/04/07 00:00 [received] PHST- 2018/06/05 00:00 [accepted] PHST- 2018/09/11 06:00 [entrez] PHST- 2018/09/11 06:00 [pubmed] PHST- 2018/11/07 06:00 [medline] PHST- 2018/09/03 00:00 [pmc-release] AID - S2059798318008318 [pii] AID - ud5004 [pii] AID - 10.1107/S2059798318008318 [doi] PST - ppublish SO - Acta Crystallogr D Struct Biol. 2018 Sep 1;74(Pt 9):841-851. doi: 10.1107/S2059798318008318. Epub 2018 Sep 3. PMID- 30171801 OWN - NLM STAT- MEDLINE DCOM- 20190502 LR - 20201209 IS - 1865-1682 (Electronic) IS - 1865-1674 (Print) IS - 1865-1674 (Linking) VI - 66 IP - 2 DP - 2019 Mar TI - Retrospective detection and phylogenetic analysis of swine acute diarrhoea syndrome coronavirus in pigs in southern China. PG - 687-695 LID - 10.1111/tbed.13008 [doi] AB - Swine acute diarrhoea syndrome coronavirus (SADS-CoV), a novel coronavirus, was first discovered in southern China in January 2017 and caused a large scale outbreak of fatal diarrheal disease in piglets. Here, we conducted a retrospective investigation of 236 samples from 45 swine farms with a clinical history of diarrheal disease to evaluate the emergence and the distribution of SADS-CoV in pigs in China. Our results suggest that SADS-CoV has emerged in China at least since August 2016. Meanwhile, we detected a prevalence of SADS-CoV (43.53%), porcine deltacoronavirus (8.83%), porcine epidemic diarrhoea virus (PEDV) (78.25%), rotavirus (21.77%), and transmissible gastroenteritis virus (0%), and we also found the co-infection of SADS-CoV and PEDV occurred most frequently with the rate of 17.65%. We screened and obtained two new complete genomes, five N and five S genes of SADS-CoV. Phylogenetic analysis based on these sequences revealed that all SADS-CoV sequences in this study clustered with previously reported SADS-CoV strains to form a well defined branch that grouped with the bat coronavirus HKU2 strains. This study is the first retrospective investigation for SADS-CoV and provides the epidemiological information of this new virus in China, which highlights the urgency to develop effective measures to control SADS-CoV. CI - © 2018 Blackwell Verlag GmbH. FAU - Zhou, Ling AU - Zhou L AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Sun, Yuan AU - Sun Y AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Lan, Tian AU - Lan T AD - College of Animal Science, South China Agricultural University, Guangzhou, China. FAU - Wu, Ruiting AU - Wu R AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Chen, Junwei AU - Chen J AD - Guangdong Wen's Foodstuffs Group Co., Ltd., Yunfu, Guangdong, China. FAU - Wu, Zixian AU - Wu Z AD - Guangdong Wen's Foodstuffs Group Co., Ltd., Yunfu, Guangdong, China. FAU - Xie, Qingmei AU - Xie Q AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Zhang, Xiangbin AU - Zhang X AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. AD - Guangdong Wen's Foodstuffs Group Co., Ltd., Yunfu, Guangdong, China. FAU - Ma, Jingyun AU - Ma J AUID- ORCID: 0000-0001-6285-312X AD - College of Animal Science, South China Agricultural University, Guangzhou, China. AD - Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. LA - eng SI - GENBANK/MF094681 SI - GENBANK/MF094684 SI - GENBANK/MG605090 SI - GENBANK/MG605091 SI - GENBANK/MG605087 SI - GENBANK/MG605088 SI - GENBANK/MG605089 SI - GENBANK/MG775251 SI - GENBANK/MG775253 SI - GENBANK/MG605084 SI - GENBANK/MG605085 SI - GENBANK/MG605086 SI - GENBANK/MG775250 SI - GENBANK/MG775252 SI - GENBANK/MF167434 SI - GENBANK/MF370205 GR - 2016YFD0501304/National Key Research and Development Program of China/ PT - Journal Article DEP - 20190109 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 RN - 0 (Coronavirus Nucleocapsid Proteins) RN - 0 (Nucleocapsid Proteins) RN - 0 (Spike Glycoprotein, Coronavirus) RN - Swine acute diarrhea syndrome coronavirus SB - IM MH - Alphacoronavirus/classification/*genetics/*isolation & purification MH - Animals MH - China/epidemiology MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Coronavirus Nucleocapsid Proteins MH - Feces/virology MH - Female MH - *Genome, Viral MH - Nucleocapsid Proteins/analysis MH - Phylogeny MH - Polymerase Chain Reaction/veterinary MH - Prevalence MH - Retrospective Studies MH - Spike Glycoprotein, Coronavirus/analysis MH - Swine MH - Swine Diseases/*epidemiology/virology PMC - PMC7168530 OTO - NOTNLM OT - Swine Acute Diarrhoea Syndrome Coronavirus OT - phylogenetic analysis OT - prevalence OT - retrospective detection COIS- The authors declare no conflict of interests with any organization. EDAT- 2018/09/02 06:00 MHDA- 2019/05/03 06:00 PMCR- 2020/04/20 CRDT- 2018/09/02 06:00 PHST- 2018/03/19 00:00 [received] PHST- 2018/08/03 00:00 [revised] PHST- 2018/08/29 00:00 [accepted] PHST- 2018/09/02 06:00 [pubmed] PHST- 2019/05/03 06:00 [medline] PHST- 2018/09/02 06:00 [entrez] PHST- 2020/04/20 00:00 [pmc-release] AID - TBED13008 [pii] AID - 10.1111/tbed.13008 [doi] PST - ppublish SO - Transbound Emerg Dis. 2019 Mar;66(2):687-695. doi: 10.1111/tbed.13008. Epub 2019 Jan 9. PMID- 30341341 OWN - NLM STAT- MEDLINE DCOM- 20191119 LR - 20191119 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 8 IP - 1 DP - 2018 Oct 19 TI - White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats. PG - 15508 LID - 10.1038/s41598-018-33975-x [doi] LID - 15508 AB - Spillover of viruses from bats to other animals may be associated with increased contact between them, as well as increased shedding of viruses by bats. Here, we tested the prediction that little brown bats (Myotis lucifugus) co-infected with the M. lucifugus coronavirus (Myl-CoV) and with Pseudogymnoascus destructans (Pd), the fungus that causes bat white-nose syndrome (WNS), exhibit different disease severity, viral shedding and molecular responses than bats infected with only Myl-CoV or only P. destructans. We took advantage of the natural persistence of Myl-CoV in bats that were experimentally inoculated with P. destructans in a previous study. Here, we show that the intestines of virus-infected bats that were also infected with fungus contained on average 60-fold more viral RNA than bats with virus alone. Increased viral RNA in the intestines correlated with the severity of fungus-related pathology. Additionally, the intestines of bats infected with fungus exhibited different expression of mitogen-activated protein kinase pathway and cytokine related transcripts, irrespective of viral presence. Levels of coronavirus antibodies were also higher in fungal-infected bats. Our results suggest that the systemic effects of WNS may down-regulate anti-viral responses in bats persistently infected with M. lucifugus coronavirus and increase the potential of virus shedding. FAU - Davy, Christina M AU - Davy CM AD - Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada. AD - Ontario Ministry of Natural Resources and Forestry, Wildlife Research and Monitoring Section, Trent University, Peterborough, ON, Canada. FAU - Donaldson, Michael E AU - Donaldson ME AD - Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada. FAU - Subudhi, Sonu AU - Subudhi S AUID- ORCID: 0000-0002-5937-1880 AD - Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Rapin, Noreen AU - Rapin N AD - Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Warnecke, Lisa AU - Warnecke L AD - Department of Biology, University of Winnipeg, Manitoba, Canada. AD - Department of Animal Ecology and Conservation, University Hamburg, Hamburg, Hamburg, Germany. FAU - Turner, James M AU - Turner JM AD - Department of Biology, University of Winnipeg, Manitoba, Canada. AD - Institute for Land Water and Society, Charles Sturt University, Albury, New South Wales, Australia. FAU - Bollinger, Trent K AU - Bollinger TK AD - Canadian Wildlife Health Cooperative and Department of Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Kyle, Christopher J AU - Kyle CJ AD - Forensic Science Department, Trent University, Peterborough, ON, Canada. FAU - Dorville, Nicole A S-Y AU - Dorville NAS AD - Department of Biology, University of Winnipeg, Manitoba, Canada. FAU - Kunkel, Emma L AU - Kunkel EL AD - Department of Biology, University of Winnipeg, Manitoba, Canada. FAU - Norquay, Kaleigh J O AU - Norquay KJO AD - Department of Biology, University of Winnipeg, Manitoba, Canada. FAU - Dzal, Yvonne A AU - Dzal YA AD - Department of Biology, University of Winnipeg, Manitoba, Canada. FAU - Willis, Craig K R AU - Willis CKR AD - Department of Biology, University of Winnipeg, Manitoba, Canada. FAU - Misra, Vikram AU - Misra V AUID- ORCID: 0000-0001-6818-7156 AD - Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. vikram.misra@usask.ca. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20181019 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Antibodies, Viral) RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Antibodies, Viral/metabolism MH - Ascomycota/*physiology MH - Chiroptera/*microbiology/*virology MH - Coinfection/microbiology/virology MH - Coronavirus/*physiology MH - Gene Expression Regulation MH - Immunity, Innate/genetics MH - Intestines/immunology/microbiology/virology MH - Male MH - Models, Biological MH - Mycoses/*veterinary MH - RNA, Viral/metabolism MH - Virus Replication/*physiology PMC - PMC6195612 COIS- The authors declare no competing interests. EDAT- 2018/10/21 06:00 MHDA- 2019/11/20 06:00 PMCR- 2018/10/19 CRDT- 2018/10/21 06:00 PHST- 2018/07/16 00:00 [received] PHST- 2018/10/09 00:00 [accepted] PHST- 2018/10/21 06:00 [entrez] PHST- 2018/10/21 06:00 [pubmed] PHST- 2019/11/20 06:00 [medline] PHST- 2018/10/19 00:00 [pmc-release] AID - 10.1038/s41598-018-33975-x [pii] AID - 33975 [pii] AID - 10.1038/s41598-018-33975-x [doi] PST - epublish SO - Sci Rep. 2018 Oct 19;8(1):15508. doi: 10.1038/s41598-018-33975-x. PMID- 29769338 OWN - NLM STAT- MEDLINE DCOM- 20180723 LR - 20190118 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 15 DP - 2018 Aug 1 TI - The PERK Arm of the Unfolded Protein Response Negatively Regulates Transmissible Gastroenteritis Virus Replication by Suppressing Protein Translation and Promoting Type I Interferon Production. LID - 10.1128/JVI.00431-18 [doi] LID - e00431-18 AB - Coronavirus replication is closely associated with the endoplasmic reticulum (ER), the primary cellular organelle for protein synthesis, folding, and modification. ER stress is a common consequence in coronavirus-infected cells. However, how the virus-induced ER stress influences coronavirus replication and pathogenesis remains controversial. Here, we demonstrated that infection with the alphacoronavirus transmissible gastroenteritis virus (TGEV) induced ER stress and triggered the unfolded protein response (UPR) in vitro and in vivo, and ER stress negatively regulated TGEV replication in vitro Although TGEV infection activated all three UPR pathways (activating transcription factor 6 [ATF6], inositol-requiring enzyme 1 [IRE1], and protein kinase R-like ER kinase [PERK]), the virus-triggered UPR suppressed TGEV replication in both swine testicular (ST) and IPEC-J2 cells primarily through activation of the PERK-eukaryotic initiation factor 2α (eIF2α) axis, as shown by functional studies with overexpression, small interfering RNA (siRNA), or specific chemical inhibitors. Moreover, we demonstrated that PERK-eIF2α axis-mediated inhibition of TGEV replication occurs through phosphorylated eIF2α-induced overall attenuation of protein translation. In addition to direct inhibition of viral production, the PERK-eIF2α pathway activated NF-κB and then facilitated type I IFN production, resulting in TGEV suppression. Taken together, our results suggest that the TGEV-triggered PERK-eIF2α pathway negatively regulates TGEV replication and represents a vital aspect of host innate responses to invading pathogens.IMPORTANCE The induction of ER stress is a common outcome in cells infected with coronaviruses. The UPR initiated by ER stress is actively involved in viral replication and modulates the host innate responses to the invading viruses, but these underlying mechanisms remain incompletely understood. We show here that infection with the alphacoronavirus TGEV elicited ER stress in vitro and in vivo, and the UPR PERK-eIF2α branch was predominantly responsible for the suppression of TGEV replication by ER stress. Furthermore, the PERK-eIF2α axis inhibited TGEV replication through direct inhibition of viral proteins due to global translation inhibition and type I IFN induction. These findings highlight a critical role of the UPR PERK-eIF2α pathway in modulating host innate immunity and coronavirus replication. CI - Copyright © 2018 American Society for Microbiology. FAU - Xue, Mei AU - Xue M AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Fu, Fang AU - Fu F AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Ma, Yanlong AU - Ma Y AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Zhang, Xin AU - Zhang X AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Li, Liang AU - Li L AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Feng, Li AU - Feng L AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China fengli_h@163.com liupinghuang@caas.cn. FAU - Liu, Pinghuang AU - Liu P AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China fengli_h@163.com liupinghuang@caas.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180717 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Activating Transcription Factor 6) RN - 0 (Eukaryotic Initiation Factor-2) RN - 0 (Interferon Type I) RN - EC 2.7.11.1 (PERK kinase) RN - EC 2.7.11.1 (eIF-2 Kinase) SB - IM MH - Activating Transcription Factor 6/genetics/metabolism MH - Animals MH - Cell Line MH - Eukaryotic Initiation Factor-2/genetics/metabolism MH - Interferon Type I/*biosynthesis/genetics MH - *Protein Biosynthesis MH - Swine MH - Transmissible gastroenteritis virus/*physiology MH - *Unfolded Protein Response MH - Virus Replication/*physiology MH - eIF-2 Kinase/genetics/*metabolism PMC - PMC6052291 OTO - NOTNLM OT - endoplasmic reticulum stress OT - interferon OT - protein kinase R-like ER kinase OT - translation attenuation OT - transmissible gastroenteritis virus OT - unfolded protein response EDAT- 2018/05/18 06:00 MHDA- 2018/07/24 06:00 PMCR- 2019/01/17 CRDT- 2018/05/18 06:00 PHST- 2018/03/14 00:00 [received] PHST- 2018/04/30 00:00 [accepted] PHST- 2018/05/18 06:00 [pubmed] PHST- 2018/07/24 06:00 [medline] PHST- 2018/05/18 06:00 [entrez] PHST- 2019/01/17 00:00 [pmc-release] AID - JVI.00431-18 [pii] AID - 00431-18 [pii] AID - 10.1128/JVI.00431-18 [doi] PST - epublish SO - J Virol. 2018 Jul 17;92(15):e00431-18. doi: 10.1128/JVI.00431-18. Print 2018 Aug 1. PMID- 30356097 OWN - NLM STAT- MEDLINE DCOM- 20191211 LR - 20221207 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 8 IP - 1 DP - 2018 Oct 24 TI - Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis. PG - 15701 LID - 10.1038/s41598-018-34171-7 [doi] LID - 15701 AB - Severe acute respiratory syndrome coronavirus (SARS-CoV) emerged in 2002 as a highly transmissible pathogenic human betacoronavirus. The viral spike glycoprotein (S) utilizes angiotensin-converting enzyme 2 (ACE2) as a host protein receptor and mediates fusion of the viral and host membranes, making S essential to viral entry into host cells and host species tropism. As SARS-CoV enters host cells, the viral S is believed to undergo a number of conformational transitions as it is cleaved by host proteases and binds to host receptors. We recently developed stabilizing mutations for coronavirus spikes that prevent the transition from the pre-fusion to post-fusion states. Here, we present cryo-EM analyses of a stabilized trimeric SARS-CoV S, as well as the trypsin-cleaved, stabilized S, and its interactions with ACE2. Neither binding to ACE2 nor cleavage by trypsin at the S1/S2 cleavage site impart large conformational changes within stabilized SARS-CoV S or expose the secondary cleavage site, S2'. FAU - Kirchdoerfer, Robert N AU - Kirchdoerfer RN AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA. FAU - Wang, Nianshuang AU - Wang N AD - Department of Biochemistry and Cellular Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA. AD - Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, 78712, USA. FAU - Pallesen, Jesper AU - Pallesen J AUID- ORCID: 0000-0002-3270-1587 AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA. FAU - Wrapp, Daniel AU - Wrapp D AD - Department of Biochemistry and Cellular Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA. AD - Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, 78712, USA. FAU - Turner, Hannah L AU - Turner HL AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA. FAU - Cottrell, Christopher A AU - Cottrell CA AUID- ORCID: 0000-0002-3364-3083 AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA. FAU - Corbett, Kizzmekia S AU - Corbett KS AD - Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD, 20814, USA. FAU - Graham, Barney S AU - Graham BS AD - Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD, 20814, USA. FAU - McLellan, Jason S AU - McLellan JS AD - Department of Biochemistry and Cellular Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA. AD - Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, 78712, USA. FAU - Ward, Andrew B AU - Ward AB AD - Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA. andrew@scripps.edu. LA - eng GR - K99 AI123498/AI/NIAID NIH HHS/United States GR - R00 AI123498/AI/NIAID NIH HHS/United States GR - R01 AI127521/AI/NIAID NIH HHS/United States GR - S10 OD021634/OD/NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20181024 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 9DLQ4CIU6V (Proline) RN - EC 3.4.- (Peptide Hydrolases) RN - EC 3.4.15.1 (Peptidyl-Dipeptidase A) RN - EC 3.4.17.23 (ACE2 protein, human) RN - EC 3.4.17.23 (Angiotensin-Converting Enzyme 2) RN - EC 3.4.21.4 (Trypsin) SB - IM EIN - Sci Rep. 2018 Dec 10;8(1):17823. doi: 10.1038/s41598-018-36918-8. PMID: 30531867 MH - Angiotensin-Converting Enzyme 2 MH - Binding Sites MH - Cryoelectron Microscopy MH - Glycosylation MH - HEK293 Cells MH - Humans MH - Mutation MH - Peptide Hydrolases/chemistry MH - Peptidyl-Dipeptidase A/chemistry MH - Proline/genetics MH - *Protein Stability MH - *Protein Structure, Secondary MH - *Proteolysis MH - Receptors, Virus/*chemistry MH - Severe acute respiratory syndrome-related coronavirus/chemistry MH - Spike Glycoprotein, Coronavirus/*chemistry MH - Trypsin/chemistry MH - Viral Tropism MH - Virus Internalization PMC - PMC6200764 COIS- R.N.K., N.W., J.P., H.L.T., C.A.C., K.S.C., B.S.G., J.S.M. and A.B.W. are inventors on US patent Application No. 62/412,703, entitled “Prefusion Coronavirus Spike Proteins and Their Use”. D.W. has no competing interests. EDAT- 2018/10/26 06:00 MHDA- 2019/12/18 06:00 PMCR- 2018/10/24 CRDT- 2018/10/26 06:00 PHST- 2018/07/13 00:00 [received] PHST- 2018/10/12 00:00 [accepted] PHST- 2018/10/26 06:00 [entrez] PHST- 2018/10/26 06:00 [pubmed] PHST- 2019/12/18 06:00 [medline] PHST- 2018/10/24 00:00 [pmc-release] AID - 10.1038/s41598-018-34171-7 [pii] AID - 34171 [pii] AID - 10.1038/s41598-018-34171-7 [doi] PST - epublish SO - Sci Rep. 2018 Oct 24;8(1):15701. doi: 10.1038/s41598-018-34171-7. PMID- 29579103 OWN - NLM STAT- MEDLINE DCOM- 20180705 LR - 20181114 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 13 IP - 3 DP - 2018 TI - A metagenomic viral discovery approach identifies potential zoonotic and novel mammalian viruses in Neoromicia bats within South Africa. PG - e0194527 LID - 10.1371/journal.pone.0194527 [doi] LID - e0194527 AB - Species within the Neoromicia bat genus are abundant and widely distributed in Africa. It is common for these insectivorous bats to roost in anthropogenic structures in urban regions. Additionally, Neoromicia capensis have previously been identified as potential hosts for Middle East respiratory syndrome (MERS)-related coronaviruses. This study aimed to ascertain the gastrointestinal virome of these bats, as viruses excreted in fecal material or which may be replicating in rectal or intestinal tissues have the greatest opportunities of coming into contact with other hosts. Samples were collected in five regions of South Africa over eight years. Initial virome composition was determined by viral metagenomic sequencing by pooling samples and enriching for viral particles. Libraries were sequenced on the Illumina MiSeq and NextSeq500 platforms, producing a combined 37 million reads. Bioinformatics analysis of the high throughput sequencing data detected the full genome of a novel species of the Circoviridae family, and also identified sequence data from the Adenoviridae, Coronaviridae, Herpesviridae, Parvoviridae, Papillomaviridae, Phenuiviridae, and Picornaviridae families. Metagenomic sequencing data was insufficient to determine the viral diversity of certain families due to the fragmented coverage of genomes and lack of suitable sequencing depth, as some viruses were detected from the analysis of reads-data only. Follow up conventional PCR assays targeting conserved gene regions for the Adenoviridae, Coronaviridae, and Herpesviridae families were used to confirm metagenomic data and generate additional sequences to determine genetic diversity. The complete coding genome of a MERS-related coronavirus was recovered with additional amplicon sequencing on the MiSeq platform. The new genome shared 97.2% overall nucleotide identity to a previous Neoromicia-associated MERS-related virus, also from South Africa. Conventional PCR analysis detected diverse adenovirus and herpesvirus sequences that were widespread throughout Neoromicia populations in South Africa. Furthermore, similar adenovirus sequences were detected within these populations throughout several years. With the exception of the coronaviruses, the study represents the first report of sequence data from several viral families within a Southern African insectivorous bat genus; highlighting the need for continued investigations in this regard. FAU - Geldenhuys, Marike AU - Geldenhuys M AD - Centre for Viral Zoonoses, Department of Medical Virology, University of Pretoria, Pretoria, South Africa. FAU - Mortlock, Marinda AU - Mortlock M AD - Centre for Viral Zoonoses, Department of Medical Virology, University of Pretoria, Pretoria, South Africa. FAU - Weyer, Jacqueline AU - Weyer J AD - Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa. FAU - Bezuidt, Oliver AU - Bezuidt O AD - Centre for Microbial Ecology and Genomics, University of Pretoria, Pretoria, South Africa. FAU - Seamark, Ernest C J AU - Seamark ECJ AD - AfricanBats NPC, Pretoria, South Africa. AD - Eugène Marais Chair of Wildlife Management, Mammal Research Institute, University of Pretoria, Pretoria, South Africa. FAU - Kearney, Teresa AU - Kearney T AD - Ditsong National Museum of Natural History, Pretoria, South Africa. AD - School of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa. FAU - Gleasner, Cheryl AU - Gleasner C AD - Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America. FAU - Erkkila, Tracy H AU - Erkkila TH AD - Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America. FAU - Cui, Helen AU - Cui H AD - Los Alamos National Laboratory, Los Alamos, New Mexico, United States of America. FAU - Markotter, Wanda AU - Markotter W AD - Centre for Viral Zoonoses, Department of Medical Virology, University of Pretoria, Pretoria, South Africa. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180326 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Adenoviridae/genetics/pathogenicity MH - Animals MH - Chiroptera/physiology/*virology MH - Computational Biology MH - Coronavirus/genetics/pathogenicity MH - Coronavirus Infections/veterinary/*virology MH - Gastrointestinal Tract/physiology/virology MH - Genetic Variation MH - Genome, Viral/*genetics MH - Herpesviridae/genetics/pathogenicity MH - High-Throughput Nucleotide Sequencing MH - Humans MH - Mammals/*virology MH - Metagenomics/methods MH - Phylogeny MH - Phylogeography MH - Sequence Analysis, DNA MH - South Africa MH - Zoonoses/*virology PMC - PMC5868816 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2018/03/27 06:00 MHDA- 2018/07/06 06:00 PMCR- 2018/03/26 CRDT- 2018/03/27 06:00 PHST- 2017/12/14 00:00 [received] PHST- 2018/03/05 00:00 [accepted] PHST- 2018/03/27 06:00 [entrez] PHST- 2018/03/27 06:00 [pubmed] PHST- 2018/07/06 06:00 [medline] PHST- 2018/03/26 00:00 [pmc-release] AID - PONE-D-17-43706 [pii] AID - 10.1371/journal.pone.0194527 [doi] PST - epublish SO - PLoS One. 2018 Mar 26;13(3):e0194527. doi: 10.1371/journal.pone.0194527. eCollection 2018. PMID- 28706276 OWN - NLM STAT- MEDLINE DCOM- 20190212 LR - 20240613 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 7 IP - 1 DP - 2017 Jul 13 TI - Kanyawara Virus: A Novel Rhabdovirus Infecting Newly Discovered Nycteribiid Bat Flies Infesting Previously Unknown Pteropodid Bats in Uganda. PG - 5287 LID - 10.1038/s41598-017-05236-w [doi] LID - 5287 AB - Bats are natural reservoir hosts of highly virulent pathogens such as Marburg virus, Nipah virus, and SARS coronavirus. However, little is known about the role of bat ectoparasites in transmitting and maintaining such viruses. The intricate relationship between bats and their ectoparasites suggests that ectoparasites might serve as viral vectors, but evidence to date is scant. Bat flies, in particular, are highly specialized obligate hematophagous ectoparasites that incidentally bite humans. Using next-generation sequencing, we discovered a novel ledantevirus (mononegaviral family Rhabdoviridae, genus Ledantevirus) in nycteribiid bat flies infesting pteropodid bats in western Uganda. Mitochondrial DNA analyses revealed that both the bat flies and their bat hosts belong to putative new species. The coding-complete genome of the new virus, named Kanyawara virus (KYAV), is only distantly related to that of its closest known relative, Mount Elgon bat virus, and was found at high titers in bat flies but not in blood or on mucosal surfaces of host bats. Viral genome analysis indicates unusually low CpG dinucleotide depletion in KYAV compared to other ledanteviruses and rhabdovirus groups, with KYAV displaying values similar to rhabdoviruses of arthropods. Our findings highlight the possibility of a yet-to-be-discovered diversity of potentially pathogenic viruses in bat ectoparasites. FAU - Goldberg, Tony L AU - Goldberg TL AUID- ORCID: 0000-0003-3962-4913 AD - Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, 53706, USA. tony.goldberg@wisc.edu. AD - Global Health Institute, University of Wisconsin-Madison, Madison, Wisconsin, 53706, USA. tony.goldberg@wisc.edu. AD - Department of Zoology, Makerere University, Kampala, Uganda. tony.goldberg@wisc.edu. FAU - Bennett, Andrew J AU - Bennett AJ AD - Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin, 53706, USA. FAU - Kityo, Robert AU - Kityo R AD - Department of Zoology, Makerere University, Kampala, Uganda. FAU - Kuhn, Jens H AU - Kuhn JH AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, 21702, USA. FAU - Chapman, Colin A AU - Chapman CA AD - Department of Zoology, Makerere University, Kampala, Uganda. AD - Department of Anthropology and School of Environment, McGill University, Montreal, Quebec, H3A 2T7, Canada. LA - eng GR - HHSN272200700016I/AI/NIAID NIH HHS/United States GR - R01 TW009237/TW/FIC NIH HHS/United States GR - T32 AI078985/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20170713 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 SB - IM MH - Animals MH - Chiroptera/*parasitology MH - Diptera/*virology MH - Disease Reservoirs/*parasitology/virology MH - *Genome, Viral MH - Phylogeny MH - Prevalence MH - Rhabdoviridae/*classification/genetics/*isolation & purification MH - Rhabdoviridae Infections/*epidemiology/transmission/virology PMC - PMC5509700 COIS- The authors declare that they have no competing interests. EDAT- 2017/07/15 06:00 MHDA- 2019/02/13 06:00 PMCR- 2017/07/13 CRDT- 2017/07/15 06:00 PHST- 2017/04/19 00:00 [received] PHST- 2017/05/25 00:00 [accepted] PHST- 2017/07/15 06:00 [entrez] PHST- 2017/07/15 06:00 [pubmed] PHST- 2019/02/13 06:00 [medline] PHST- 2017/07/13 00:00 [pmc-release] AID - 10.1038/s41598-017-05236-w [pii] AID - 5236 [pii] AID - 10.1038/s41598-017-05236-w [doi] PST - epublish SO - Sci Rep. 2017 Jul 13;7(1):5287. doi: 10.1038/s41598-017-05236-w. PMID- 29498228 OWN - NLM STAT- MEDLINE DCOM- 20180921 LR - 20200423 IS - 1865-1682 (Electronic) IS - 1865-1674 (Print) IS - 1865-1674 (Linking) VI - 65 IP - 4 DP - 2018 Aug TI - Coronaviruses in guano from Pteropus medius bats in Peradeniya, Sri Lanka. PG - 1122-1124 LID - 10.1111/tbed.12851 [doi] AB - Bats are a unique group of mammals well suited to be hosts for emerging viruses. With current rates of deforestation and urbanization, redistribution of bat habitats to urban and suburban areas may bring bats into closer contact with livestock and humans. Common flying fox, Pteropus medius (previously known as Pteropus giganteus), forms large communal roosts on treetops, often in close proximity to human habitation in Sri Lanka. This report describes the detection of coronavirus RNA in P. medius bat guano collected in Peradeniya, Sri Lanka. These viruses had >97% nucleotide identity with coronaviruses detected in Cynopterus sphinx, Scotophilus heathii and S. kuhlii bats in Thailand. Pteropus medius is widespread in Asia and appears to excrete group D coronaviruses, which are hitherto confined to bats; however, these findings may have public health implications in the future. CI - © 2018 Blackwell Verlag GmbH. FAU - Kudagammana, H D W S AU - Kudagammana HDWS AUID- ORCID: 0000-0003-4906-0998 AD - Faculty of Allied Health Sciences, University of Peradeniya, Peradeniya, Sri Lanka. AD - Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. FAU - Thevanesam, V AU - Thevanesam V AD - Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. FAU - Chu, D K W AU - Chu DKW AUID- ORCID: 0000-0002-9219-8979 AD - School of Public Health, University of Hong Kong, Hong Kong, Hong Kong. FAU - Eriyagama, N B AU - Eriyagama NB AD - Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. FAU - Peiris, J S M AU - Peiris JSM AD - School of Public Health, University of Hong Kong, Hong Kong, Hong Kong. FAU - Noordeen, F AU - Noordeen F AD - Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. LA - eng GR - HHSN272201500006C/AI/NIAID NIH HHS/United States PT - Journal Article DEP - 20180302 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronavirus/genetics/*isolation & purification MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Feces/*virology MH - Genome, Viral/genetics MH - Phylogeny MH - RNA, Viral/genetics MH - Sri Lanka/epidemiology MH - Thailand PMC - PMC7169738 OTO - NOTNLM OT - Sri Lanka OT - coronavirus OT - flying fox COIS- The authors of the work have no conflict of interests to disclose. EDAT- 2018/03/03 06:00 MHDA- 2018/09/22 06:00 PMCR- 2020/04/20 CRDT- 2018/03/03 06:00 PHST- 2017/09/09 00:00 [received] PHST- 2018/03/03 06:00 [pubmed] PHST- 2018/09/22 06:00 [medline] PHST- 2018/03/03 06:00 [entrez] PHST- 2020/04/20 00:00 [pmc-release] AID - TBED12851 [pii] AID - 10.1111/tbed.12851 [doi] PST - ppublish SO - Transbound Emerg Dis. 2018 Aug;65(4):1122-1124. doi: 10.1111/tbed.12851. Epub 2018 Mar 2. PMID- 31025076 OWN - NLM STAT- MEDLINE DCOM- 20190912 LR - 20200325 IS - 1432-0614 (Electronic) IS - 0175-7598 (Print) IS - 0175-7598 (Linking) VI - 103 IP - 12 DP - 2019 Jun TI - A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses. PG - 4943-4952 LID - 10.1007/s00253-019-09835-7 [doi] AB - Swine enteric coronaviruses are a group of most significant pathogens causing diarrhea in piglets with similar clinical symptoms and pathological changes. To develop a simple, rapid, accurate, and high-throughput detection method for diagnosis and differential diagnosis on swine enteric coronaviruses, specific primers and probes were designed based on the highly conserved regions of transmissible gastroenteritis virus (TGEV) N, porcine epidemic diarrhea virus (PEDV) M, porcine deltacoronavirus (PDCoV) M, and porcine enteric alphacoronavirus (PEAV) N genes respectively. A TaqMan-probe-based multiplex real-time RT-qPCR assay was developed and optimized to simultaneously detect these swine enteric coronaviruses. The results showed that the limit of detection can reach as low as 10 copies in singular real-time RT-qPCR assays and 100 copies in multiplex real-time RT-qPCR assay, with all correlation coefficients (R(2)) at above 0.99, and the amplification efficiency at between 90 and 120%. This multiplex real-time RT-qPCR assay demonstrated high sensitivity, extreme specificity, and excellent repeatability. The multiplex real-time RT-qPCR assay was then employed to detect the swine enteric coronavirus from 354 field diarrheal samples. The results manifested that TGEV and PDCoV were the main pathogens in these samples, accompanied by co-infections. This well-established multiplex real-time RT-qPCR assay provided a rapid, efficient, specific, and sensitive tool for detection of swine enteric coronaviruses. FAU - Huang, Xin AU - Huang X AD - College of Veterinary Medicine, Xinjiang Agricultural University, 311 East Noda Road, Urumqi, 830052, Xinjiang, China. AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 XuJiaPing, YanChangBu, ChengGuan District, Lanzhou, 730046, Gansu, China. FAU - Chen, Jianing AU - Chen J AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 XuJiaPing, YanChangBu, ChengGuan District, Lanzhou, 730046, Gansu, China. FAU - Yao, Gang AU - Yao G AD - College of Veterinary Medicine, Xinjiang Agricultural University, 311 East Noda Road, Urumqi, 830052, Xinjiang, China. FAU - Guo, Qingyong AU - Guo Q AD - College of Veterinary Medicine, Xinjiang Agricultural University, 311 East Noda Road, Urumqi, 830052, Xinjiang, China. FAU - Wang, Jinquan AU - Wang J AD - College of Veterinary Medicine, Xinjiang Agricultural University, 311 East Noda Road, Urumqi, 830052, Xinjiang, China. Wangjinquan163@163.com. FAU - Liu, Guangliang AU - Liu G AUID- ORCID: 0000-0001-8158-5749 AD - College of Veterinary Medicine, Xinjiang Agricultural University, 311 East Noda Road, Urumqi, 830052, Xinjiang, China. LiuGuangliang01@caas.cn. AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 XuJiaPing, YanChangBu, ChengGuan District, Lanzhou, 730046, Gansu, China. LiuGuangliang01@caas.cn. LA - eng GR - 2016YFD0500103/National Key R&D Program of China/ GR - 31572498/National Natural Science Foundation of China/ GR - 31702209/National Natural Science Foundation of China/ GR - N/A/Elite Youth Program of CAAS/ PT - Journal Article DEP - 20190426 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - 0 (DNA Primers) SB - IM MH - Animals MH - Coronavirus/classification/*isolation & purification MH - Coronavirus Infections/diagnosis/*veterinary MH - DNA Primers/genetics MH - Diagnosis, Differential MH - Diarrhea/virology MH - Feces/virology MH - Gastroenteritis, Transmissible, of Swine/diagnosis/virology MH - Limit of Detection MH - Multiplex Polymerase Chain Reaction/*methods MH - Porcine epidemic diarrhea virus/genetics/isolation & purification MH - Real-Time Polymerase Chain Reaction/*methods MH - Reproducibility of Results MH - Sensitivity and Specificity MH - Swine MH - Swine Diseases/*diagnosis/virology MH - Transmissible gastroenteritis virus/genetics/isolation & purification PMC - PMC7080015 OTO - NOTNLM OT - Diagnosis OT - Multiplex real-time RT-qPCR OT - Swine enteric coronaviruses OT - TaqMan probe COIS- The authors declare that they have no competing interests. EDAT- 2019/04/27 06:00 MHDA- 2019/09/13 06:00 PMCR- 2020/03/18 CRDT- 2019/04/27 06:00 PHST- 2019/02/20 00:00 [received] PHST- 2019/04/07 00:00 [accepted] PHST- 2019/03/29 00:00 [revised] PHST- 2019/04/27 06:00 [pubmed] PHST- 2019/09/13 06:00 [medline] PHST- 2019/04/27 06:00 [entrez] PHST- 2020/03/18 00:00 [pmc-release] AID - 10.1007/s00253-019-09835-7 [pii] AID - 9835 [pii] AID - 10.1007/s00253-019-09835-7 [doi] PST - ppublish SO - Appl Microbiol Biotechnol. 2019 Jun;103(12):4943-4952. doi: 10.1007/s00253-019-09835-7. Epub 2019 Apr 26. PMID- 28589292 OWN - NLM STAT- MEDLINE DCOM- 20180305 LR - 20190822 IS - 1995-820X (Electronic) IS - 1674-0769 (Print) IS - 1995-820X (Linking) VI - 32 IP - 3 DP - 2017 Jun TI - Detection of diverse viruses in alimentary specimens of bats in Macau. PG - 226-234 LID - 10.1007/s12250-017-3976-9 [doi] AB - Bats carry a variety of viruses, and some of them cause public health problems. Macau, which is famous for its gambling industry, has a complex population structure. The globalization in such an international metropolis has enhanced the chance of disease transmission. Therefore, surveillance of zoonotic viruses is necessary for the early warning of potential emerging infectious diseases. Here, we report the first surveillance of bat viruses in Macau. In this study, we collected 1004 samples involving 10 bat species from 7 sites from April 2015 to May 2016, and examined the presence of viruses using nucleic acid-based methods. Coronaviruses, adenoviruses and paramyxoviruses were detected in these samples, with a high prevalence of coronaviruses. While, none was positive for hepatitis A virus, hepatitis E virus or hantavirus. Co-infections are not common in those bat species, but coronavirus HKU6 and adenovirus can be found commonly occurred in Myotis ricketti. FAU - Liang, Jie AU - Liang J AD - College of Life Science, Guangzhou University, Guangzhou, 510006, China. AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China. FAU - Yang, Xing-Lou AU - Yang XL AD - CAS Key Laboratory of Special Pathogens, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Li, Bei AU - Li B AD - CAS Key Laboratory of Special Pathogens, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Liu, Qi AU - Liu Q AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China. FAU - Zhang, Qin AU - Zhang Q AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China. FAU - Liu, Hui AU - Liu H AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China. FAU - Kan, Hon-Pio AU - Kan HP AD - Macau Civic and Municipal Affairs Bureau, Macao SAR, China. FAU - Wong, Kai-Chin AU - Wong KC AD - Macau Civic and Municipal Affairs Bureau, Macao SAR, China. FAU - Chek, Si-Nga AU - Chek SN AD - Macau Civic and Municipal Affairs Bureau, Macao SAR, China. FAU - He, Xiangyang AU - He X AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China. FAU - Peng, Xingwen AU - Peng X AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China. FAU - Shi, Zheng-Li AU - Shi ZL AD - CAS Key Laboratory of Special Pathogens, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Wu, Yi AU - Wu Y AUID- ORCID: 0000-0003-1010-8640 AD - College of Life Science, Guangzhou University, Guangzhou, 510006, China. wuyizhouq@263.net. FAU - Zhang, Libiao AU - Zhang L AUID- ORCID: 0000-0002-6919-7695 AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China. zhanglb@gdei.gd.cn. LA - eng PT - Journal Article DEP - 20170601 PL - Netherlands TA - Virol Sin JT - Virologica Sinica JID - 101514185 SB - IM MH - Animals MH - *Biodiversity MH - Chiroptera MH - Feces/*virology MH - Macau MH - Viruses/*classification/*isolation & purification PMC - PMC6598931 OTO - NOTNLM OT - adenovirus OT - bat virus OT - coronavirus OT - paramyxovirus OT - viral detection EDAT- 2017/06/08 06:00 MHDA- 2018/03/06 06:00 PMCR- 2018/06/01 CRDT- 2017/06/08 06:00 PHST- 2017/03/12 00:00 [received] PHST- 2017/05/09 00:00 [accepted] PHST- 2017/06/08 06:00 [pubmed] PHST- 2018/03/06 06:00 [medline] PHST- 2017/06/08 06:00 [entrez] PHST- 2018/06/01 00:00 [pmc-release] AID - 10.1007/s12250-017-3976-9 [pii] AID - 3976 [pii] AID - 10.1007/s12250-017-3976-9 [doi] PST - ppublish SO - Virol Sin. 2017 Jun;32(3):226-234. doi: 10.1007/s12250-017-3976-9. Epub 2017 Jun 1. PMID- 30969980 OWN - NLM STAT- MEDLINE DCOM- 20191219 LR - 20200617 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 14 IP - 4 DP - 2019 TI - A viral metagenomic survey identifies known and novel mammalian viruses in bats from Saudi Arabia. PG - e0214227 LID - 10.1371/journal.pone.0214227 [doi] LID - e0214227 AB - Bats are implicated as natural reservoirs for a wide range of zoonotic viruses including SARS and MERS coronaviruses, Ebola, Marburg, Nipah, Hendra, Rabies and other lyssaviruses. Accordingly, many One Health surveillance and viral discovery programs have focused on bats. In this report we present viral metagenomic data from bats collected in the Kingdom of Saudi Arabia [KSA]. Unbiased high throughput sequencing of fecal samples from 72 bat individuals comprising four species; lesser mouse-tailed bat (Rhinopoma hardwickii), Egyptian tomb bat (Taphozous perforatus), straw-colored fruit bat (Eidolon helvum), and Egyptian fruit bat (Rousettus aegyptiacus) revealed molecular evidence of a diverse set of viral families: Picornaviridae (hepatovirus, teschovirus, parechovirus), Reoviridae (rotavirus), Polyomaviridae (polyomavirus), Papillomaviridae (papillomavirus), Astroviridae (astrovirus), Caliciviridae (sapovirus), Coronaviridae (coronavirus), Adenoviridae (adenovirus), Paramyxoviridae (paramyxovirus), and unassigned mononegavirales (chuvirus). Additionally, we discovered a bastro-like virus (Middle East Hepe-Astrovirus), with a genomic organization similar to Hepeviridae. However, since it shared homology with Hepeviridae and Astroviridae at ORF1 and in ORF2, respectively, the newly discovered Hepe-Astrovirus may represent a phylogenetic bridge between Hepeviridae and Astroviridae. FAU - Mishra, Nischay AU - Mishra N AUID- ORCID: 0000-0002-1177-9641 AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Fagbo, Shamsudeen F AU - Fagbo SF AD - One Health Unit, Executive Directorate for Surveillance and Response, National Center for Disease Prevention and Control, Riyadh, Saudi Arabia. FAU - Alagaili, Abdulaziz N AU - Alagaili AN AD - KSU Mammals Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia. FAU - Nitido, Adam AU - Nitido A AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Williams, Simon H AU - Williams SH AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Ng, James AU - Ng J AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Lee, Bohyun AU - Lee B AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Durosinlorun, Abdulkareem AU - Durosinlorun A AD - Federal Ministry of Agriculture and Rural Development, Kaduna, Nigeria. FAU - Garcia, Joel A AU - Garcia JA AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Jain, Komal AU - Jain K AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Kapoor, Vishal AU - Kapoor V AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Epstein, Jonathan H AU - Epstein JH AD - EcoHealth Alliance, New York, New York, United States of America. FAU - Briese, Thomas AU - Briese T AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. FAU - Memish, Ziad A AU - Memish ZA AD - The College of Medicine, Al faisal University & Prince Mohammed Bin Abdulaziz Hospital, Ministry of Health, Riyadh, Kingdom of Saudi Arabia. FAU - Olival, Kevin J AU - Olival KJ AD - EcoHealth Alliance, New York, New York, United States of America. FAU - Lipkin, W Ian AU - Lipkin WI AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, United States of America. LA - eng GR - U19 AI109761/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20190410 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Animals MH - Caliciviridae/genetics/isolation & purification MH - Chiroptera/genetics/*virology MH - Egypt MH - Feces/virology MH - High-Throughput Nucleotide Sequencing MH - Humans MH - Mammals/virology MH - Metagenome/*genetics MH - *Metagenomics MH - Middle East MH - Middle East Respiratory Syndrome Coronavirus MH - Paramyxoviridae/genetics/isolation & purification MH - *Phylogeny MH - Picornaviridae/genetics/isolation & purification MH - RNA Viruses/genetics MH - Rotavirus/genetics/isolation & purification MH - Saudi Arabia PMC - PMC6457491 COIS- The authors have declared that no competing interests exist. EDAT- 2019/04/11 06:00 MHDA- 2019/12/20 06:00 PMCR- 2019/04/10 CRDT- 2019/04/11 06:00 PHST- 2018/10/10 00:00 [received] PHST- 2019/03/09 00:00 [accepted] PHST- 2019/04/11 06:00 [entrez] PHST- 2019/04/11 06:00 [pubmed] PHST- 2019/12/20 06:00 [medline] PHST- 2019/04/10 00:00 [pmc-release] AID - PONE-D-18-27977 [pii] AID - 10.1371/journal.pone.0214227 [doi] PST - epublish SO - PLoS One. 2019 Apr 10;14(4):e0214227. doi: 10.1371/journal.pone.0214227. eCollection 2019. PMID- 29427670 OWN - NLM STAT- MEDLINE DCOM- 20180926 LR - 20210109 IS - 1879-0984 (Electronic) IS - 0166-0934 (Print) IS - 0166-0934 (Linking) VI - 255 DP - 2018 May TI - Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs. PG - 66-70 LID - S0166-0934(17)30719-X [pii] LID - 10.1016/j.jviromet.2018.02.002 [doi] AB - Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus which was first reported in southern China in 2017. It can cause severe diarrhea disease in pigs. In order to detect this new emerging virus rapidly and reliably, a TaqMan-based real-time RT-PCR assay was established in this study. Specific primers and probe were designed and synthesized based on the conserved region within the N gene of the viral genome. Results showed that the lowest limit of detection was 3.0 × 10(1) copies/μL. This approach was specific for SADS-CoV, and there were no cross-reaction observed against other 15 swine viruses. It was 10 times more sensitive than the conventional PCR and gave higher SADS-CoV positive detection rate (70.69%, 123/174) than the conventional PCR (51.15%, 89/174) from clinical samples. These data indicated that the TaqMan-based real-time RT-PCR assay established here was an effective method with high sensitivity, specificity and reproducibility for faster and more accurate detection and quantification of SADS-CoV. CI - Copyright © 2018 Elsevier B.V. All rights reserved. FAU - Zhou, Ling AU - Zhou L AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Sun, Yuan AU - Sun Y AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Wu, Jiao-Ling AU - Wu JL AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Mai, Kai-Jie AU - Mai KJ AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Chen, Gui-Hua AU - Chen GH AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Wu, Zi-Xian AU - Wu ZX AD - Guangdong Wen's Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing, 527400, Guangdong, China. FAU - Bai, Yang AU - Bai Y AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Li, Di AU - Li D AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Zhou, Zhi-Hai AU - Zhou ZH AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Cheng, Jian AU - Cheng J AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Wu, Rui-Ting AU - Wu RT AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. FAU - Zhang, Xiang-Bin AU - Zhang XB AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China; Hog Production Division, Guangdong Wen's Foodstuffs Group Co., Ltd., Xinxing, 527439, China. Electronic address: Zhangxb@scau.edu.cn. FAU - Ma, Jing-Yun AU - Ma JY AD - College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China. Electronic address: majy2400@scau.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180207 PL - Netherlands TA - J Virol Methods JT - Journal of virological methods JID - 8005839 RN - Swine acute diarrhea syndrome coronavirus SB - IM MH - Alphacoronavirus/*genetics MH - Animals MH - Coronavirus Infections/*veterinary MH - Diarrhea/*virology MH - *Real-Time Polymerase Chain Reaction/methods MH - Reproducibility of Results MH - Sensitivity and Specificity MH - Swine MH - Swine Diseases/*diagnosis/*virology PMC - PMC7113665 OTO - NOTNLM OT - Diagnosis OT - Quantification OT - SADS-CoV OT - TaqMan-based real-time RT-PCR EDAT- 2018/02/11 06:00 MHDA- 2018/09/27 06:00 PMCR- 2018/02/07 CRDT- 2018/02/11 06:00 PHST- 2017/11/12 00:00 [received] PHST- 2018/01/21 00:00 [revised] PHST- 2018/02/02 00:00 [accepted] PHST- 2018/02/11 06:00 [pubmed] PHST- 2018/09/27 06:00 [medline] PHST- 2018/02/11 06:00 [entrez] PHST- 2018/02/07 00:00 [pmc-release] AID - S0166-0934(17)30719-X [pii] AID - 10.1016/j.jviromet.2018.02.002 [doi] PST - ppublish SO - J Virol Methods. 2018 May;255:66-70. doi: 10.1016/j.jviromet.2018.02.002. Epub 2018 Feb 7. PMID- 29258555 OWN - NLM STAT- MEDLINE DCOM- 20180717 LR - 20181113 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 14 IP - 1 DP - 2017 Dec 19 TI - Detection and full genome characterization of two beta CoV viruses related to Middle East respiratory syndrome from bats in Italy. PG - 239 LID - 10.1186/s12985-017-0907-1 [doi] LID - 239 AB - BACKGROUND: Middle East respiratory syndrome coronavirus (MERS-CoV), which belongs to beta group of coronavirus, can infect multiple host species and causes severe diseases in humans. Multiple surveillance and phylogenetic studies suggest a bat origin. In this study, we describe the detection and full genome characterization of two CoVs closely related to MERS-CoV from two Italian bats, Pipistrellus kuhlii and Hypsugo savii. METHODS: Pool of viscera were tested by a pan-coronavirus RT-PCR. Virus isolation was attempted by inoculation in different cell lines. Full genome sequencing was performed using the Ion Torrent platform and phylogenetic trees were performed using IQtree software. Similarity plots of CoV clade c genomes were generated by using SSE v1.2. The three dimensional macromolecular structure (3DMMS) of the receptor binding domain (RBD) in the S protein was predicted by sequence-homology method using the protein data bank (PDB). RESULTS: Both samples resulted positive to the pan-coronavirus RT-PCR (IT-batCoVs) and their genome organization showed identical pattern of MERS CoV. Phylogenetic analysis showed a monophyletic group placed in the Beta2c clade formed by MERS-CoV sequences originating from humans and camels and bat-related sequences from Africa, Italy and China. The comparison of the secondary and 3DMMS of the RBD of IT-batCoVs with MERS, HKU4 and HKU5 bat sequences showed two aa deletions located in a region corresponding to the external subdomain of MERS-RBD in IT-batCoV and HKU5 RBDs. CONCLUSIONS: This study reported two beta CoVs closely related to MERS that were obtained from two bats belonging to two commonly recorded species in Italy (P. kuhlii and H. savii). The analysis of the RBD showed similar structure in IT-batCoVs and HKU5 respect to HKU4 sequences. Since the RBD domain of HKU4 but not HKU5 can bind to the human DPP4 receptor for MERS-CoV, it is possible to suggest also for IT-batCoVs the absence of DPP4-binding potential. More surveillance studies are needed to better investigate the potential intermediate hosts that may play a role in the interspecies transmission of known and currently unknown coronaviruses with particular attention to the S protein and the receptor specificity and binding affinity. FAU - Moreno, Ana AU - Moreno A AUID- ORCID: 0000-0002-8497-9708 AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. anamaria.morenomartin@izsler.it. FAU - Lelli, Davide AU - Lelli D AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. FAU - de Sabato, Luca AU - de Sabato L AD - Department of Food Safety, Istituto Superiore di Sanità. Nutrition and Veterinary Public Health, Viale Regina Elena 299, 00161, Rome, Italy. AD - Dept. of Sciences, University Roma Tre, Viale Guglielmo Marconi 446, 00146, Rome, Italy. FAU - Zaccaria, Guendalina AU - Zaccaria G AD - Department of Food Safety, Istituto Superiore di Sanità. Nutrition and Veterinary Public Health, Viale Regina Elena 299, 00161, Rome, Italy. FAU - Boni, Arianna AU - Boni A AD - Department of Food Safety, Istituto Superiore di Sanità. Nutrition and Veterinary Public Health, Viale Regina Elena 299, 00161, Rome, Italy. FAU - Sozzi, Enrica AU - Sozzi E AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. FAU - Prosperi, Alice AU - Prosperi A AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. FAU - Lavazza, Antonio AU - Lavazza A AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. FAU - Cella, Eleonora AU - Cella E AD - University Campus Bio-Medico of Rome, Via Álvaro del Portillo, 21, 00128, Rome, Italy. FAU - Castrucci, Maria Rita AU - Castrucci MR AD - Department of Food Safety, Istituto Superiore di Sanità. Nutrition and Veterinary Public Health, Viale Regina Elena 299, 00161, Rome, Italy. FAU - Ciccozzi, Massimo AU - Ciccozzi M AD - University Campus Bio-Medico of Rome, Via Álvaro del Portillo, 21, 00128, Rome, Italy. FAU - Vaccari, Gabriele AU - Vaccari G AD - Department of Food Safety, Istituto Superiore di Sanità. Nutrition and Veterinary Public Health, Viale Regina Elena 299, 00161, Rome, Italy. LA - eng PT - Journal Article DEP - 20171219 PL - England TA - Virol J JT - Virology journal JID - 101231645 RN - 0 (RNA, Viral) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM EIN - Virol J. 2018 Jan 12;15(1):10. doi: 10.1186/s12985-018-0921-y. PMID: 29329554 MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Chiroptera/*virology MH - Genome, Viral/*genetics MH - Italy MH - Middle East Respiratory Syndrome Coronavirus/chemistry/*classification/*genetics/isolation & purification MH - *Phylogeny MH - Polymerase Chain Reaction MH - Protein Interaction Domains and Motifs MH - Protein Structure, Tertiary/genetics MH - RNA, Viral/genetics MH - Sequence Analysis, RNA MH - Spike Glycoprotein, Coronavirus/chemistry/genetics PMC - PMC5735805 OTO - NOTNLM OT - Bats OT - Full genome sequencing OT - Italy OT - MERS-like Beta-CoV viruses OT - Phylogenetic and molecular analyses COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable CONSENT FOR PUBLICATION: Not applicable COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2017/12/21 06:00 MHDA- 2018/07/18 06:00 PMCR- 2017/12/19 CRDT- 2017/12/21 06:00 PHST- 2017/10/20 00:00 [received] PHST- 2017/12/06 00:00 [accepted] PHST- 2017/12/21 06:00 [entrez] PHST- 2017/12/21 06:00 [pubmed] PHST- 2018/07/18 06:00 [medline] PHST- 2017/12/19 00:00 [pmc-release] AID - 10.1186/s12985-017-0907-1 [pii] AID - 907 [pii] AID - 10.1186/s12985-017-0907-1 [doi] PST - epublish SO - Virol J. 2017 Dec 19;14(1):239. doi: 10.1186/s12985-017-0907-1. PMID- 26633467 OWN - NLM STAT- MEDLINE DCOM- 20160929 LR - 20201209 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 7 IP - 12 DP - 2015 Dec 2 TI - Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats. PG - 6279-90 LID - 10.3390/v7122937 [doi] AB - Bats are a reservoir for a diverse range of viruses, including coronaviruses (CoVs). To determine the presence of CoVs in French bats, fecal samples were collected between July and August of 2014 from four bat species in seven different locations around the city of Bourges in France. We present for the first time the presence of alpha-CoVs in French Pipistrellus pipistrellus bat species with an estimated prevalence of 4.2%. Based on the analysis of a fragment of the RNA-dependent RNA polymerase (RdRp) gene, phylogenetic analyses show that alpha-CoVs sequences detected in French bats are closely related to other European bat alpha-CoVs. Phylogeographic analyses of RdRp sequences show that several CoVs strains circulate in European bats: (i) old strains detected that have probably diverged a long time ago and are detected in different bat subspecies; (ii) strains detected in Myotis and Pipistrellus bat species that have more recently diverged. Our findings support previous observations describing the complexity of the detected CoVs in bats worldwide. FAU - Goffard, Anne AU - Goffard A AD - Molecular & Cellular Virology, University Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 8204-CIIL-Centre d'Infection et d'Immunité de Lille, Bâtiment IBL. 1 rue du Pr. Calmette CS 50447, 59021 Lille Cedex, France. anne.goffard@univ-lille2.fr. FAU - Demanche, Christine AU - Demanche C AD - Bacterial Respiratory Infections: Pertussis and Tuberculosis, University Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 8204-CIIL-Centre d'Infection et d'Immunité de Lille, F-59000 Lille, France. christine.demanche@univ-lille2.fr. FAU - Arthur, Laurent AU - Arthur L AD - Museum d'Histoire Naturelle de Bourges, Les Rives d'Auron, allée René Ménard, 18000 Bourges, France. laurent.arthur@ville-bourges.fr. FAU - Pinçon, Claire AU - Pinçon C AD - University Lille, CHU Lille, EA 2694-Santé publique: épidémiologie et qualité des soins, F-59000 Lille, France. claire.pincon@univ-lille2.fr. FAU - Michaux, Johan AU - Michaux J AD - Conservation Genetics Unit, Institute of Botany (B. 22), University Liège, 4000 Liège, Belgium. johan.michaux@ulg.ac.be. AD - CIRAD TA C-22/E-Campus international de Baillarguet, 34398 Montpellier Cedex 5, France. johan.michaux@ulg.ac.be. FAU - Dubuisson, Jean AU - Dubuisson J AD - Molecular & Cellular Virology, University Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 8204-CIIL-Centre d'Infection et d'Immunité de Lille, Bâtiment IBL. 1 rue du Pr. Calmette CS 50447, 59021 Lille Cedex, France. jean.dubuisson@ibl.cnrs.fr. LA - eng PT - Journal Article DEP - 20151202 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (RNA, Viral) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Animals MH - Chiroptera/*virology MH - Cluster Analysis MH - Coronavirus/*classification/*genetics/isolation & purification MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Feces/virology MH - France/epidemiology MH - *Phylogeography MH - Prevalence MH - RNA, Viral/genetics MH - RNA-Dependent RNA Polymerase/genetics MH - Sequence Analysis, DNA MH - Sequence Homology PMC - PMC4690861 OTO - NOTNLM OT - Europe OT - alphacoronavirus OT - bats OT - coronavirus OT - molecular characterization OT - phylogenetic analysis OT - phylogeographic analysis EDAT- 2015/12/04 06:00 MHDA- 2016/09/30 06:00 PMCR- 2015/12/01 CRDT- 2015/12/04 06:00 PHST- 2015/09/14 00:00 [received] PHST- 2015/11/17 00:00 [revised] PHST- 2015/11/23 00:00 [accepted] PHST- 2015/12/04 06:00 [entrez] PHST- 2015/12/04 06:00 [pubmed] PHST- 2016/09/30 06:00 [medline] PHST- 2015/12/01 00:00 [pmc-release] AID - v7122937 [pii] AID - viruses-07-02937 [pii] AID - 10.3390/v7122937 [doi] PST - epublish SO - Viruses. 2015 Dec 2;7(12):6279-90. doi: 10.3390/v7122937. PMID- 25409519 OWN - NLM STAT- MEDLINE DCOM- 20150709 LR - 20240613 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 9 IP - 11 DP - 2014 TI - CD26/DPP4 cell-surface expression in bat cells correlates with bat cell susceptibility to Middle East respiratory syndrome coronavirus (MERS-CoV) infection and evolution of persistent infection. PG - e112060 LID - 10.1371/journal.pone.0112060 [doi] LID - e112060 AB - Middle East respiratory syndrome coronavirus (MERS-CoV) is a recently isolated betacoronavirus identified as the etiologic agent of a frequently fatal disease in Western Asia, Middle East respiratory syndrome. Attempts to identify the natural reservoirs of MERS-CoV have focused in part on dromedaries. Bats are also suspected to be reservoirs based on frequent detection of other betacoronaviruses in these mammals. For this study, ten distinct cell lines derived from bats of divergent species were exposed to MERS-CoV. Plaque assays, immunofluorescence assays, and transmission electron microscopy confirmed that six bat cell lines can be productively infected. We found that the susceptibility or resistance of these bat cell lines directly correlates with the presence or absence of cell surface-expressed CD26/DPP4, the functional human receptor for MERS-CoV. Human anti-CD26/DPP4 antibodies inhibited infection of susceptible bat cells in a dose-dependent manner. Overexpression of human CD26/DPP4 receptor conferred MERS-CoV susceptibility to resistant bat cell lines. Finally, sequential passage of MERS-CoV in permissive bat cells established persistent infection with concomitant downregulation of CD26/DPP4 surface expression. Together, these results imply that bats indeed could be among the MERS-CoV host spectrum, and that cellular restriction of MERS-CoV is determined by CD26/DPP4 expression rather than by downstream restriction factors. FAU - Caì, Yíngyún AU - Caì Y AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Yú, Shu Qìng AU - Yú SQ AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Postnikova, Elena N AU - Postnikova EN AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Mazur, Steven AU - Mazur S AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Bernbaum, John G AU - Bernbaum JG AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Burk, Robin AU - Burk R AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Zhāng, Téngfēi AU - Zhāng T AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Radoshitzky, Sheli R AU - Radoshitzky SR AD - United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, United States of America. FAU - Müller, Marcel A AU - Müller MA AD - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany. FAU - Jordan, Ingo AU - Jordan I AD - ProBioGen AG, Berlin, Germany. FAU - Bollinger, Laura AU - Bollinger L AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Hensley, Lisa E AU - Hensley LE AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Jahrling, Peter B AU - Jahrling PB AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. FAU - Kuhn, Jens H AU - Kuhn JH AD - Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America. LA - eng GR - HHSN272200700016I/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20141119 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Antibodies, Monoclonal) RN - 0 (Receptors, Virus) RN - EC 3.4.14.5 (DPP4 protein, human) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Animals MH - Antibodies, Monoclonal/*immunology MH - Cell Line MH - Chiroptera/*virology MH - Chlorocebus aethiops MH - Coronavirus Infections/transmission/virology MH - Dipeptidyl Peptidase 4/*metabolism MH - Disease Reservoirs/virology MH - Disease Susceptibility MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/pathogenicity/*physiology MH - Receptors, Virus/metabolism MH - Vero Cells MH - Viral Tropism PMC - PMC4237331 COIS- Competing Interests: Dr. Ingo Jordan is employed by ProBioGen and is an author on patent application WO 2009/109377 A1 covering also the R05T and R06E cell lines. Both cell lines are freely available for research without restrictions. The authors confirm that Dr. Jens H. Kuhn is a PLOS ONE Editorial Board member. This does not alter the authors' adherence to PLOS ONE Editorial policies and criteria. EDAT- 2014/11/20 06:00 MHDA- 2015/07/15 06:00 PMCR- 2014/11/19 CRDT- 2014/11/20 06:00 PHST- 2014/06/30 00:00 [received] PHST- 2014/10/12 00:00 [accepted] PHST- 2014/11/20 06:00 [entrez] PHST- 2014/11/20 06:00 [pubmed] PHST- 2015/07/15 06:00 [medline] PHST- 2014/11/19 00:00 [pmc-release] AID - PONE-D-14-29261 [pii] AID - 10.1371/journal.pone.0112060 [doi] PST - epublish SO - PLoS One. 2014 Nov 19;9(11):e112060. doi: 10.1371/journal.pone.0112060. eCollection 2014. PMID- 29346682 OWN - NLM STAT- MEDLINE DCOM- 20190912 LR - 20200403 IS - 1537-6613 (Electronic) IS - 0022-1899 (Print) IS - 0022-1899 (Linking) VI - 218 IP - 2 DP - 2018 Jun 20 TI - Receptor Usage of a Novel Bat Lineage C Betacoronavirus Reveals Evolution of Middle East Respiratory Syndrome-Related Coronavirus Spike Proteins for Human Dipeptidyl Peptidase 4 Binding. PG - 197-207 LID - 10.1093/infdis/jiy018 [doi] AB - Although bats are known to harbor Middle East Respiratory Syndrome coronavirus (MERS-CoV)-related viruses, the role of bats in the evolutionary origin and pathway remains obscure. We identified a novel MERS-CoV-related betacoronavirus, Hp-BatCoV HKU25, from Chinese pipistrelle bats. Although it is closely related to MERS-CoV in most genome regions, its spike protein occupies a phylogenetic position between that of Ty-BatCoV HKU4 and Pi-BatCoV HKU5. Because Ty-BatCoV HKU4 but not Pi-BatCoV HKU5 can use the MERS-CoV receptor human dipeptidyl peptidase 4 (hDPP4) for cell entry, we tested the ability of Hp-BatCoV HKU25 to bind and use hDPP4. The HKU25-receptor binding domain (RBD) can bind to hDPP4 protein and hDPP4-expressing cells, but it does so with lower efficiency than that of MERS-RBD. Pseudovirus assays showed that HKU25-spike can use hDPP4 for entry to hDPP4-expressing cells, although with lower efficiency than that of MERS-spike and HKU4-spike. Our findings support a bat origin of MERS-CoV and suggest that bat CoV spike proteins may have evolved in a stepwise manner for binding to hDPP4. FAU - Lau, Susanna K P AU - Lau SKP AD - State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, China. AD - Department of Microbiology The University of Hong Kong, China. AD - Carol Yu Centre for Infection The University of Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China. FAU - Zhang, Libiao AU - Zhang L AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, and Guangdong Institute of Applied Biological Resources, Guangzhou, Guangdong Province, China. FAU - Luk, Hayes K H AU - Luk HKH AD - Department of Microbiology The University of Hong Kong, China. FAU - Xiong, Lifeng AU - Xiong L AD - Department of Microbiology The University of Hong Kong, China. FAU - Peng, Xingwen AU - Peng X AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, and Guangdong Institute of Applied Biological Resources, Guangzhou, Guangdong Province, China. FAU - Li, Kenneth S M AU - Li KSM AD - Department of Microbiology The University of Hong Kong, China. FAU - He, Xiangyang AU - He X AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, and Guangdong Institute of Applied Biological Resources, Guangzhou, Guangdong Province, China. FAU - Zhao, Pyrear Su-Hui AU - Zhao PS AD - Department of Microbiology The University of Hong Kong, China. FAU - Fan, Rachel Y Y AU - Fan RYY AD - Department of Microbiology The University of Hong Kong, China. FAU - Wong, Antonio C P AU - Wong ACP AD - Department of Microbiology The University of Hong Kong, China. FAU - Ahmed, Syed Shakeel AU - Ahmed SS AD - Department of Microbiology The University of Hong Kong, China. FAU - Cai, Jian-Piao AU - Cai JP AD - Department of Microbiology The University of Hong Kong, China. FAU - Chan, Jasper F W AU - Chan JFW AD - State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, China. AD - Department of Microbiology The University of Hong Kong, China. AD - Carol Yu Centre for Infection The University of Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China. FAU - Sun, Yinyan AU - Sun Y AD - National Institute of Biological Sciences, Zhongguancun Life Science Park, Changping, Beijing, China. FAU - Jin, Dongyan AU - Jin D AD - School of Biomedical Sciences, The University of Hong Kong, China. FAU - Chen, Honglin AU - Chen H AD - State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, China. AD - Department of Microbiology The University of Hong Kong, China. AD - Carol Yu Centre for Infection The University of Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China. FAU - Lau, Terrence C K AU - Lau TCK AD - Department of Biomedical Sciences, City University of Hong Kong, China. FAU - Kok, Raven K H AU - Kok RKH AD - State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, China. AD - Department of Microbiology The University of Hong Kong, China. AD - Carol Yu Centre for Infection The University of Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China. FAU - Li, Wenhui AU - Li W AD - National Institute of Biological Sciences, Zhongguancun Life Science Park, Changping, Beijing, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, China. AD - Department of Microbiology The University of Hong Kong, China. AD - Carol Yu Centre for Infection The University of Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China. FAU - Woo, Patrick C Y AU - Woo PCY AD - State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong, China. AD - Department of Microbiology The University of Hong Kong, China. AD - Carol Yu Centre for Infection The University of Hong Kong, China. AD - Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Infect Dis JT - The Journal of infectious diseases JID - 0413675 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.14.5 (DPP4 protein, human) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Animals MH - Betacoronavirus/classification/genetics/isolation & purification/*physiology MH - *Chiroptera MH - Dipeptidyl Peptidase 4/*metabolism MH - *Evolution, Molecular MH - HEK293 Cells MH - Humans MH - Phylogeny MH - Protein Binding MH - Receptors, Virus/*metabolism MH - Sequence Analysis, DNA MH - Spike Glycoprotein, Coronavirus/genetics/*metabolism MH - *Virus Internalization PMC - PMC7107427 EDAT- 2018/01/19 06:00 MHDA- 2019/09/13 06:00 PMCR- 2019/06/20 CRDT- 2018/01/19 06:00 PHST- 2017/11/20 00:00 [received] PHST- 2018/01/15 00:00 [accepted] PHST- 2018/01/19 06:00 [pubmed] PHST- 2019/09/13 06:00 [medline] PHST- 2018/01/19 06:00 [entrez] PHST- 2019/06/20 00:00 [pmc-release] AID - 4810771 [pii] AID - jiy018 [pii] AID - 10.1093/infdis/jiy018 [doi] PST - ppublish SO - J Infect Dis. 2018 Jun 20;218(2):197-207. doi: 10.1093/infdis/jiy018. PMID- 30185587 OWN - NLM STAT- MEDLINE DCOM- 20190327 LR - 20190429 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 22 DP - 2018 Nov 15 TI - The Coronavirus Transmissible Gastroenteritis Virus Evades the Type I Interferon Response through IRE1α-Mediated Manipulation of the MicroRNA miR-30a-5p/SOCS1/3 Axis. LID - 10.1128/JVI.00728-18 [doi] LID - e00728-18 AB - In host innate immunity, type I interferons (IFN-I) are major antiviral molecules, and coronaviruses have evolved diverse strategies to counter the IFN-I response during infection. Transmissible gastroenteritis virus (TGEV), a member of the Alphacoronavirus family, induces endoplasmic reticulum (ER) stress and significant IFN-I production after infection. However, how TGEV evades the IFN-I antiviral response despite the marked induction of endogenous IFN-I has remained unclear. Inositol-requiring enzyme 1 α (IRE1α), a highly conserved ER stress sensor with both kinase and RNase activities, is involved in the IFN response. In this study, IRE1α facilitated TGEV replication via downmodulating the host microRNA (miR) miR-30a-5p abundance. miR-30a-5p normally enhances IFN-I antiviral activity by directly targeting the negative regulators of Janus family kinase (JAK)-signal transducer and activator of transcription (STAT), the suppressor of cytokine signaling protein 1 (SOCS1), and SOCS3. Furthermore, TGEV infection increased SOCS1 and SOCS3 expression, which dampened the IFN-I antiviral response and facilitated TGEV replication. Importantly, compared with mock infection, TGEV infection in vivo resulted in decreased miR-30a-5p levels and significantly elevated SOCS1 and SOCS3 expression in the piglet ileum. Taken together, our data reveal a new strategy used by TGEV to escape the IFN-I response by engaging the IRE1α-miR-30a-5p/SOCS1/3 axis, thus improving our understanding of how TGEV escapes host innate immune defenses.IMPORTANCE Type I interferons (IFN-I) play essential roles in restricting viral infections. Coronavirus infection induces ER stress and the interferon response, which reflects different adaptive cellular processes. An understanding of how coronavirus-elicited ER stress is actively involved in viral replication and manipulates the host IFN-I response has remained elusive. Here, TGEV inhibited host miR-30a-5p via the ER stress sensor IRE1α, which led to the increased expression of negative regulators of JAK-STAT signaling cascades, namely, SOCS1 and SOCS3. Increased SOCS1 or SOCS3 expression impaired the IFN-I antiviral response, promoting TGEV replication. These findings enhance our understanding of the strategies used by coronaviruses to antagonize IFN-I innate immunity via IRE1α-mediated manipulation of the miR-30a-5p/SOCS axis, highlighting the crucial role of IRE1α in innate antiviral resistance and the potential of IRE1α as a novel target against coronavirus infection. CI - Copyright © 2018 American Society for Microbiology. FAU - Ma, Yanlong AU - Ma Y AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Wang, Changlin AU - Wang C AD - Department of Urology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China. FAU - Xue, Mei AU - Xue M AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Fu, Fang AU - Fu F AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Zhang, Xin AU - Zhang X AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Li, Liang AU - Li L AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Yin, Lingdan AU - Yin L AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Xu, Wanhai AU - Xu W AD - Department of Urology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China. FAU - Feng, Li AU - Feng L AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China fengli@caas.cn liupinghuang@caas.cn. FAU - Liu, Pinghuang AU - Liu P AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China fengli@caas.cn liupinghuang@caas.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181029 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Interferon Type I) RN - 0 (MicroRNAs) RN - 0 (Suppressor of Cytokine Signaling 1 Protein) RN - 0 (Suppressor of Cytokine Signaling 3 Protein) RN - EC 2.7.10.2 (Janus Kinases) RN - EC 3.1.- (Endoribonucleases) SB - IM MH - Animals MH - Cell Line MH - Endoplasmic Reticulum Stress/genetics/*immunology MH - Endoribonucleases/*metabolism MH - Host-Pathogen Interactions/immunology MH - Immune Evasion/genetics/*immunology MH - Immunity, Innate/immunology MH - Interferon Type I/*immunology MH - Janus Kinases/metabolism MH - MicroRNAs/*genetics MH - Suppressor of Cytokine Signaling 1 Protein/*metabolism MH - Suppressor of Cytokine Signaling 3 Protein/*metabolism MH - Sus scrofa MH - Transmissible gastroenteritis virus/genetics/*immunology MH - Virus Replication/physiology PMC - PMC6206482 OTO - NOTNLM OT - IRE1α OT - SOCS OT - miR-30a-5p OT - transmissible gastroenteritis virus (TGEV) OT - type I interferon EDAT- 2018/09/07 06:00 MHDA- 2019/03/28 06:00 PMCR- 2019/04/29 CRDT- 2018/09/07 06:00 PHST- 2018/04/26 00:00 [received] PHST- 2018/08/23 00:00 [accepted] PHST- 2018/09/07 06:00 [pubmed] PHST- 2019/03/28 06:00 [medline] PHST- 2018/09/07 06:00 [entrez] PHST- 2019/04/29 00:00 [pmc-release] AID - JVI.00728-18 [pii] AID - 00728-18 [pii] AID - 10.1128/JVI.00728-18 [doi] PST - epublish SO - J Virol. 2018 Oct 29;92(22):e00728-18. doi: 10.1128/JVI.00728-18. Print 2018 Nov 15. PMID- 30903596 OWN - NLM STAT- MEDLINE DCOM- 20191114 LR - 20200325 IS - 1867-0342 (Electronic) IS - 1867-0334 (Print) IS - 1867-0334 (Linking) VI - 11 IP - 2 DP - 2019 Jun TI - Glass Wool Concentration Optimization for the Detection of Enveloped and Non-enveloped Waterborne Viruses. PG - 184-192 LID - 10.1007/s12560-019-09378-0 [doi] AB - An extremely affordable virus concentration method based on adsorption-elution to glass wool and subsequent reconcentration through polyethylene glycol 6000 (PEG) precipitation was optimized to recover not only non-enveloped viruses but also enveloped viruses. Hepatitis A virus (HAV) and transmissible gastroenteritis virus (TGEV) were employed as surrogates for naked and enveloped viruses, respectively, to set up the methodology. Initial experimentation in small-volume samples showed that both types of particles readily adsorbed to the positively charged glass wool but were poorly detached from it through standard elution with 0.05 M glycine with 3% of beef extract buffer, pH 9.5, with elution efficiencies of 7.2% and 2.6%, for HAV and TGEV, respectively. To improve the recovery of enveloped viruses, several modifications in the elution were assayed: increasing the elution pH, extending glass wool and eluent contact time, adding a detergent, or performing the elution by recirculation or under agitation. Considering practicability and performance, recircularization of the eluent at pH 11.0 for 20 min was the elution procedure of choice, with efficiencies of 25.7% and 18.8% for HAV and TGEV in 50 L of water. Additionally, employing 20% PEG instead of 10% for virus reconcentration improved recoveries up to 47% and 51%, respectively. The optimized procedure was applied to detect naturally occurring HAV and coronaviruses in surface water of Wadi Hanifa, Riyadh. HAV was detected in 38% of the samples, while one sample was positive for an alphacoronavirus. This cheap virus detection system enables the comprehensive surveillance of viruses present in water samples. FAU - Blanco, Albert AU - Blanco A AD - Enteric Virus Laboratory, Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain. AD - Institute of Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain. FAU - Abid, Islem AU - Abid I AD - Botany and Microbiology Department, Science College, King Saud University, Riyadh, Saudi Arabia. FAU - Al-Otaibi, Nawal AU - Al-Otaibi N AD - Botany and Microbiology Department, Science College, King Saud University, Riyadh, Saudi Arabia. FAU - Pérez-Rodríguez, Francisco José AU - Pérez-Rodríguez FJ AD - Enteric Virus Laboratory, Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain. AD - Institute of Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain. FAU - Fuentes, Cristina AU - Fuentes C AD - Enteric Virus Laboratory, Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain. AD - Institute of Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain. FAU - Guix, Susana AU - Guix S AD - Enteric Virus Laboratory, Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain. AD - Institute of Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain. FAU - Pintó, Rosa M AU - Pintó RM AD - Enteric Virus Laboratory, Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain. AD - Institute of Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain. FAU - Bosch, Albert AU - Bosch A AUID- ORCID: 0000-0002-8111-9059 AD - Enteric Virus Laboratory, Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Spain. abosch@ub.edu. AD - Institute of Nutrition and Food Safety (INSA·UB), University of Barcelona, Barcelona, Spain. abosch@ub.edu. LA - eng GR - 12-ENV2528-02/MAARIFAH/International GR - Food-FP7-311846/FP7 International Cooperation/International PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190321 PL - United States TA - Food Environ Virol JT - Food and environmental virology JID - 101483831 SB - IM MH - Adsorption MH - Fresh Water/*virology MH - Glass/*chemistry MH - Hepatitis A virus/*chemistry/isolation & purification MH - Transmissible gastroenteritis virus/*chemistry/isolation & purification MH - Virology/instrumentation/*methods MH - Viruses/chemistry/isolation & purification PMC - PMC7090506 OTO - NOTNLM OT - Coronavirus OT - Enveloped viruses OT - Hepatitis A virus OT - Non-enveloped viruses OT - Water concentration COIS- The authors declare that they have no conflict of interest. EDAT- 2019/03/25 06:00 MHDA- 2019/11/15 06:00 PMCR- 2020/03/24 CRDT- 2019/03/24 06:00 PHST- 2018/07/26 00:00 [received] PHST- 2019/03/13 00:00 [accepted] PHST- 2019/03/25 06:00 [pubmed] PHST- 2019/11/15 06:00 [medline] PHST- 2019/03/24 06:00 [entrez] PHST- 2020/03/24 00:00 [pmc-release] AID - 10.1007/s12560-019-09378-0 [pii] AID - 9378 [pii] AID - 10.1007/s12560-019-09378-0 [doi] PST - ppublish SO - Food Environ Virol. 2019 Jun;11(2):184-192. doi: 10.1007/s12560-019-09378-0. Epub 2019 Mar 21. PMID- 25648965 OWN - NLM STAT- MEDLINE DCOM- 20170418 LR - 20170418 IS - 1544-2217 (Electronic) IS - 0300-9858 (Linking) VI - 52 IP - 6 DP - 2015 Nov TI - Necrotizing Enteritis and Hyperammonemic Encephalopathy Associated With Equine Coronavirus Infection in Equids. PG - 1148-56 LID - 10.1177/0300985814568683 [doi] AB - Equine coronavirus (ECoV) is a Betacoronavirus recently associated clinically and epidemiologically with emerging outbreaks of pyrogenic, enteric, and/or neurologic disease in horses in the United States, Japan, and Europe. We describe the pathologic, immunohistochemical, ultrastructural, and molecular findings in 2 horses and 1 donkey that succumbed to natural infection with ECoV. One horse and the donkey (case Nos. 1, 3) had severe diffuse necrotizing enteritis with marked villous attenuation, epithelial cell necrosis at the tips of the villi, neutrophilic and fibrinous extravasation into the small intestinal lumen (pseudomembrane formation), as well as crypt necrosis, microthrombosis, and hemorrhage. The other horse (case No. 2) had hyperammonemic encephalopathy with Alzheimer type II astrocytosis throughout the cerebral cortex. ECoV was detected by quantitative polymerase chain reaction in small intestinal tissue, contents, and/or feces, and coronavirus antigen was detected by immunohistochemistry in the small intestine in all cases. Coronavirus-like particles characterized by spherical, moderately electron lucent, enveloped virions with distinct peplomer-like structures projecting from the surface were detected by negatively stained transmission electron microscopy in small intestine in case No. 1, and transmission electron microscopy of fixed small intestinal tissue from the same case revealed similar 85- to 100-nm intracytoplasmic particles located in vacuoles and free in the cytoplasm of unidentified (presumably epithelial) cells. Sequence comparison showed 97.9% to 99.0% sequence identity with the ECoV-NC99 and Tokachi09 strains. All together, these results indicate that ECoV is associated with necrotizing enteritis and hyperammonemic encephalopathy in equids. CI - © The Author(s) 2015. FAU - Giannitti, F AU - Giannitti F AD - California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA fgiannitti@yahoo.com. FAU - Diab, S AU - Diab S AD - California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA. FAU - Mete, A AU - Mete A AD - California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA. FAU - Stanton, J B AU - Stanton JB AD - Department of Veterinary Microbiology and Pathology and Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman, WA, USA. FAU - Fielding, L AU - Fielding L AD - Loomis Basin Equine Medical Center, Loomis, CA, USA. FAU - Crossley, B AU - Crossley B AD - California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA. FAU - Sverlow, K AU - Sverlow K AD - California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA. FAU - Fish, S AU - Fish S AD - California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA. FAU - Mapes, S AU - Mapes S AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, USA. FAU - Scott, L AU - Scott L AD - Idaho Equine Hospital, Nampa, ID, USA. FAU - Pusterla, N AU - Pusterla N AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, USA. LA - eng PT - Case Reports PT - Journal Article DEP - 20150203 PL - United States TA - Vet Pathol JT - Veterinary pathology JID - 0312020 SB - IM MH - Animals MH - Base Sequence MH - Brain Diseases/pathology/*veterinary/virology MH - Coronavirus/genetics/*immunology/isolation & purification MH - Coronavirus Infections/pathology/*veterinary/virology MH - Enteritis/pathology/*veterinary/virology MH - *Equidae MH - Feces/virology MH - Female MH - Horse Diseases/*pathology/virology MH - Horses MH - Hyperammonemia/veterinary MH - Intestine, Small/pathology/virology MH - Molecular Sequence Data MH - Necrosis/veterinary MH - Sequence Analysis, DNA/veterinary OTO - NOTNLM OT - betacoronavirus OT - encephalopathy OT - enteritis OT - equine coronavirus OT - horse OT - hyperammonemia OT - immunohistochemistry OT - infectious disease EDAT- 2015/02/05 06:00 MHDA- 2017/04/19 06:00 CRDT- 2015/02/05 06:00 PHST- 2015/02/05 06:00 [entrez] PHST- 2015/02/05 06:00 [pubmed] PHST- 2017/04/19 06:00 [medline] AID - 0300985814568683 [pii] AID - 10.1177/0300985814568683 [doi] PST - ppublish SO - Vet Pathol. 2015 Nov;52(6):1148-56. doi: 10.1177/0300985814568683. Epub 2015 Feb 3. PMID- 26907329 OWN - NLM STAT- MEDLINE DCOM- 20161107 LR - 20181113 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 8 IP - 3 DP - 2016 Feb 23 TI - Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus Strains. PG - 55 LID - 10.3390/v8030055 [doi] LID - 55 AB - Porcine epidemic diarrhea virus (PEDV), a member of Alphacoronavirus, has caused huge economic losses for the global pork industry recently. The spike (S) protein mediates PEDV entry into host cells. Herein, we investigated the interactions between the S protein and its receptor porcine aminopeptidase N (pAPN) or co-receptor sugars. The C-terminal domain (CTD) of the S1 domain is bound to pAPN. The prototype strain demonstrated similar receptor-binding activity compared with the variant field isolate. Three loops at the tips of the β-barrel domains did not play crucial roles in the PEDV S-pAPN association, indicating that PEDV conforms to a different receptor recognition model compared with transmissible gastroenteritis virus (TGEV), porcine respiratory CoV (PRCV), and human coronavirus NL63 (HCoV-NL63). The N-terminal domain (NTD) of the PEDV S1 domain could bind sugar, a possible co-receptor for PEDV. The prototype strain exhibited weaker sugar-binding activity compared with the variant field isolate. Strategies targeting the receptor binding domain (RBD) may be helpful for developing vaccines or antiviral drugs for PEDV. Understanding the differences in receptor binding between the prototype and the variant strains may provide insight into PEDV pathogenesis. FAU - Deng, Feng AU - Deng F AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. dengfeng207@163.com. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. dengfeng207@163.com. FAU - Ye, Gang AU - Ye G AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. ehuangliu@webmail.hzau.edu.cn. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. ehuangliu@webmail.hzau.edu.cn. FAU - Liu, Qianqian AU - Liu Q AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. liuqq563705@163.com. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. liuqq563705@163.com. FAU - Navid, Muhammad Tariq AU - Navid MT AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. dr_tariqnaveed@hotmail.com. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. dr_tariqnaveed@hotmail.com. FAU - Zhong, Xiaoli AU - Zhong X AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. Shirleyandyueyue@163.com. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Shirleyandyueyue@163.com. FAU - Li, Youwen AU - Li Y AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. lyw_lk@163.com. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. lyw_lk@163.com. FAU - Wan, Chunyun AU - Wan C AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. supwzfu@163.com. FAU - Xiao, Shaobo AU - Xiao S AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. vet@mail.hzau.edu.cn. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. vet@mail.hzau.edu.cn. FAU - He, Qigai AU - He Q AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. heqigai@yahoo.com. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. heqigai@yahoo.com. FAU - Fu, Zhen F AU - Fu ZF AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. zhenfu@uga.edu. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. zhenfu@uga.edu. AD - Departments of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. zhenfu@uga.edu. FAU - Peng, Guiqing AU - Peng G AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. penggq@mail.hzau.edu.cn. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. penggq@mail.hzau.edu.cn. AD - The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China. penggq@mail.hzau.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160223 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.11.2 (CD13 Antigens) SB - IM MH - Animals MH - CD13 Antigens/*metabolism MH - Cell Line MH - Porcine epidemic diarrhea virus/*physiology MH - Protein Binding MH - Protein Interaction Domains and Motifs MH - Protein Interaction Mapping MH - Receptors, Virus/*metabolism MH - Spike Glycoprotein, Coronavirus/genetics/*metabolism MH - Swine PMC - PMC4810246 OTO - NOTNLM OT - CHGD-01 strain OT - CV777 strain OT - PEDV OT - RBD OT - S protein OT - pAPN OT - sugar EDAT- 2016/02/26 06:00 MHDA- 2016/11/08 06:00 PMCR- 2016/03/01 CRDT- 2016/02/25 06:00 PHST- 2015/08/28 00:00 [received] PHST- 2016/02/04 00:00 [revised] PHST- 2016/02/16 00:00 [accepted] PHST- 2016/02/25 06:00 [entrez] PHST- 2016/02/26 06:00 [pubmed] PHST- 2016/11/08 06:00 [medline] PHST- 2016/03/01 00:00 [pmc-release] AID - v8030055 [pii] AID - viruses-08-00055 [pii] AID - 10.3390/v8030055 [doi] PST - epublish SO - Viruses. 2016 Feb 23;8(3):55. doi: 10.3390/v8030055. PMID- 29669833 OWN - NLM STAT- MEDLINE DCOM- 20180730 LR - 20241109 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 13 DP - 2018 Jul 1 TI - Discovery of Novel Bat Coronaviruses in South China That Use the Same Receptor as Middle East Respiratory Syndrome Coronavirus. LID - 10.1128/JVI.00116-18 [doi] LID - e00116-18 AB - Middle East respiratory syndrome coronavirus (MERS-CoV) has represented a human health threat since 2012. Although several MERS-related CoVs that belong to the same species as MERS-CoV have been identified from bats, they do not use the MERS-CoV receptor, dipeptidyl peptidase 4 (DPP4). Here, we screened 1,059 bat samples from at least 30 bat species collected in different regions in south China and identified 89 strains of lineage C betacoronaviruses, including Tylonycteris pachypus coronavirus HKU4, Pipistrellus pipistrelluscoronavirus HKU5, and MERS-related CoVs. We sequenced the full-length genomes of two positive samples collected from the great evening bat, Ia io, from Guangdong Province. The two genomes were highly similar and exhibited genomic structures identical to those of other lineage C betacoronaviruses. While they exhibited genome-wide nucleotide identities of only 75.3 to 81.2% with other MERS-related CoVs, their gene-coding regions were highly similar to their counterparts, except in the case of the spike proteins. Further protein-protein interaction assays demonstrated that the spike proteins of these MERS-related CoVs bind to the receptor DPP4. Recombination analysis suggested that the newly discovered MERS-related CoVs have acquired their spike genes from a DPP4-recognizing bat coronavirus HKU4. Our study provides further evidence that bats represent the evolutionary origins of MERS-CoV.IMPORTANCE Previous studies suggested that MERS-CoV originated in bats. However, its evolutionary path from bats to humans remains unclear. In this study, we discovered 89 novel lineage C betacoronaviruses in eight bat species. We provide evidence of a MERS-related CoV derived from the great evening bat that uses the same host receptor as human MERS-CoV. This virus also provides evidence for a natural recombination event between the bat MERS-related CoV and another bat coronavirus, HKU4. Our study expands the host ranges of MERS-related CoV and represents an important step toward establishing bats as the natural reservoir of MERS-CoV. These findings may lead to improved epidemiological surveillance of MERS-CoV and the prevention and control of the spread of MERS-CoV to humans. CI - Copyright © 2018 American Society for Microbiology. FAU - Luo, Chu-Ming AU - Luo CM AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China. AD - University of Chinese Academy of Sciences, Beijing, China. AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Wang, Ning AU - Wang N AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Yang, Xing-Lou AU - Yang XL AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China. FAU - Liu, Hai-Zhou AU - Liu HZ AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China. FAU - Zhang, Wei AU - Zhang W AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China. FAU - Li, Bei AU - Li B AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China. FAU - Hu, Ben AU - Hu B AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China. FAU - Peng, Cheng AU - Peng C AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China. FAU - Geng, Qi-Bin AU - Geng QB AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA. FAU - Zhu, Guang-Jian AU - Zhu GJ AD - EcoHealth Alliance, New York, New York, USA. FAU - Li, Fang AU - Li F AD - Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, USA lifang@umn.edu zlshi@wh.iov.cn. FAU - Shi, Zheng-Li AU - Shi ZL AUID- ORCID: 0000-0001-8089-163X AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China lifang@umn.edu zlshi@wh.iov.cn. LA - eng GR - R01 AI089728/AI/NIAID NIH HHS/United States GR - R01 AI110700/AI/NIAID NIH HHS/United States GR - R01 AI110964/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20180613 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Receptors, Virus) RN - 0 (Viral Proteins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Chiroptera/genetics/*virology MH - Coronavirus Infections/transmission/*veterinary/virology MH - *Evolution, Molecular MH - *Genome, Viral MH - Host Specificity MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/classification/*pathogenicity MH - Phylogeny MH - Receptors, Virus/genetics/*metabolism MH - Sequence Homology MH - Viral Proteins/genetics/*metabolism PMC - PMC6002729 OTO - NOTNLM OT - MERS-related coronavirus OT - bat OT - dipeptidyl peptidase 4 OT - virus discovery EDAT- 2018/04/20 06:00 MHDA- 2018/07/31 06:00 PMCR- 2018/12/13 CRDT- 2018/04/20 06:00 PHST- 2018/01/22 00:00 [received] PHST- 2018/04/03 00:00 [accepted] PHST- 2018/04/20 06:00 [pubmed] PHST- 2018/07/31 06:00 [medline] PHST- 2018/04/20 06:00 [entrez] PHST- 2018/12/13 00:00 [pmc-release] AID - JVI.00116-18 [pii] AID - 00116-18 [pii] AID - 10.1128/JVI.00116-18 [doi] PST - epublish SO - J Virol. 2018 Jun 13;92(13):e00116-18. doi: 10.1128/JVI.00116-18. Print 2018 Jul 1. PMID- 26584511 OWN - NLM STAT- MEDLINE DCOM- 20161006 LR - 20240328 IS - 1567-7257 (Electronic) IS - 1567-1348 (Print) IS - 1567-1348 (Linking) VI - 37 DP - 2016 Jan TI - Insectivorous bats carry host specific astroviruses and coronaviruses across different regions in Germany. PG - 108-16 LID - S1567-1348(15)30040-X [pii] LID - 10.1016/j.meegid.2015.11.010 [doi] AB - Recently several infectious agents with a zoonotic potential have been detected in different bat species. However, there is still a lack of knowledge on the transmission dynamics within and between bat species, as well as from bats to other mammals. To better understand these processes, it is important to compare the phylogenetic relationships between different agents to that of their respective hosts. In this study, we analysed more than 950 urine, faeces and oral swab samples collected from 653 bats from mainly four species (Myotis nattereri, Myotis bechsteinii, Myotis daubentonii, and Plecotus auritus) for the presence of coronavirus, paramyxovirus and astrovirus related nucleic acids located in three different regions of Germany. Using hemi-nested reverse transcriptase (RT)-PCR amplification of fragments within the highly conserved regions of the respective RNA dependent RNA polymerase (RdRp) genes, we detected astrovirus sequences at an overall detection rate of 25.8% of the analysed animals, with a maximum of 65% in local populations. The detection rates for coronaviruses and paramyxoviruses were distinctly lower, ranging between 1.4% and 3.1%. Interestingly, the sequence similarities in samples collected from the same bat species in different geographical areas were distinctly larger than the sequence similarities between samples from different species sampled at the same location. This indicates that host specificity may be more important than host ecology for the presence of certain viruses in bats. CI - Copyright © 2015 Elsevier B.V. All rights reserved. FAU - Fischer, Kerstin AU - Fischer K AD - Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Suedufer 10, 17493 Greifswald-Insel Riems, Germany. Electronic address: Kerstin.fischer@fli.bund.de. FAU - Zeus, Veronika AU - Zeus V AD - Ernst-Moritz-Arndt Universität Greifswald, Zoological Institute and Museum, Johann Sebastian Bach-Str. 11/12, 17489 Greifswald, Germany. Electronic address: zeusv@uni-greifswald.de. FAU - Kwasnitschka, Linda AU - Kwasnitschka L AD - Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Suedufer 10, 17493 Greifswald-Insel Riems, Germany. FAU - Kerth, Gerald AU - Kerth G AD - Ernst-Moritz-Arndt Universität Greifswald, Zoological Institute and Museum, Johann Sebastian Bach-Str. 11/12, 17489 Greifswald, Germany. Electronic address: gerald.kerth@uni-greifswald.de. FAU - Haase, Martin AU - Haase M AD - Ernst-Moritz-Arndt Universität Greifswald, Zoological Institute and Museum, Soldmannstraße 23, 17489 Greifswald, Germany. Electronic address: mhaase@uni-greifswald.de. FAU - Groschup, Martin H AU - Groschup MH AD - Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Suedufer 10, 17493 Greifswald-Insel Riems, Germany. Electronic address: martin.groschup@fli.bund.de. FAU - Balkema-Buschmann, Anne AU - Balkema-Buschmann A AD - Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Suedufer 10, 17493 Greifswald-Insel Riems, Germany. Electronic address: anne.buschmann@fli.bund.de. LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151114 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 RN - 0 (Viral Proteins) RN - EC 2.7.7.48 (RNA-Dependent RNA Polymerase) SB - IM MH - Animals MH - Avastrovirus/classification/*genetics/*isolation & purification MH - Chiroptera/*virology MH - Coronavirus/classification/*genetics/*isolation & purification MH - Feces/virology MH - Germany MH - Host Specificity MH - Mouth/virology MH - Phylogeny MH - Phylogeography MH - RNA-Dependent RNA Polymerase/genetics MH - Urine/virology MH - Viral Proteins/genetics PMC - PMC7106178 OTO - NOTNLM OT - Astroviruses OT - Coronaviruses OT - Host specificity OT - Insectivore bats OT - Paramyxoviruses EDAT- 2015/11/21 06:00 MHDA- 2016/10/08 06:00 PMCR- 2015/11/14 CRDT- 2015/11/21 06:00 PHST- 2015/06/02 00:00 [received] PHST- 2015/10/21 00:00 [revised] PHST- 2015/11/12 00:00 [accepted] PHST- 2015/11/21 06:00 [entrez] PHST- 2015/11/21 06:00 [pubmed] PHST- 2016/10/08 06:00 [medline] PHST- 2015/11/14 00:00 [pmc-release] AID - S1567-1348(15)30040-X [pii] AID - 10.1016/j.meegid.2015.11.010 [doi] PST - ppublish SO - Infect Genet Evol. 2016 Jan;37:108-16. doi: 10.1016/j.meegid.2015.11.010. Epub 2015 Nov 14. PMID- 27213718 OWN - NLM STAT- MEDLINE DCOM- 20170119 LR - 20221207 IS - 1865-1682 (Electronic) IS - 1865-1674 (Print) IS - 1865-1674 (Linking) VI - 63 IP - 4 DP - 2016 Aug TI - Detection of Severe Acute Respiratory Syndrome-Like, Middle East Respiratory Syndrome-Like Bat Coronaviruses and Group H Rotavirus in Faeces of Korean Bats. PG - 365-72 LID - 10.1111/tbed.12515 [doi] AB - Bat species around the world have recently been recognized as major reservoirs of several zoonotic viruses, such as severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), Nipah virus and Hendra virus. In this study, consensus primer-based reverse transcriptase polymerase chain reactions (RT-PCRs) and high-throughput sequencing were performed to investigate viruses in bat faecal samples collected at 11 natural bat habitat sites from July to December 2015 in Korea. Diverse coronaviruses were first detected in Korean bat faeces, including alphacoronaviruses, SARS-CoV-like and MERS-CoV-like betacoronaviruses. In addition, we identified a novel bat rotavirus belonging to group H rotavirus which has only been described in human and pigs until now. Therefore, our results suggest the need for continuing surveillance and additional virological studies in domestic bat. CI - © 2016 Blackwell Verlag GmbH. FAU - Kim, H K AU - Kim HK AD - Research Center for Viral Infectious Diseases and Control, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. FAU - Yoon, S-W AU - Yoon SW AD - Research Center for Viral Infectious Diseases and Control, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. FAU - Kim, D-J AU - Kim DJ AD - Research Center for Viral Infectious Diseases and Control, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. FAU - Koo, B-S AU - Koo BS AD - Research Center for Viral Infectious Diseases and Control, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. FAU - Noh, J Y AU - Noh JY AD - Research Center for Viral Infectious Diseases and Control, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. FAU - Kim, J H AU - Kim JH AD - Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon, Korea. FAU - Choi, Y G AU - Choi YG AD - The Korean Institute of Biospeleology, Daejeon, Korea. FAU - Na, W AU - Na W AD - Research Center for Viral Infectious Diseases and Control, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. AD - Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Korea. FAU - Chang, K-T AU - Chang KT AD - Research Center for Viral Infectious Diseases and Control, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. FAU - Song, D AU - Song D AD - Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Korea. FAU - Jeong, D G AU - Jeong DG AD - Research Center for Viral Infectious Diseases and Control, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea. AD - Bio-Analytical Science Division, Korea University of Science and Technology (UST), Daejeon, Korea. LA - eng SI - GENBANK/AB576625 SI - GENBANK/AB576629 SI - GENBANK/AB576631 SI - GENBANK/AB576634 SI - GENBANK/DQ648828 SI - GENBANK/DQ648841 SI - GENBANK/DQ648849 SI - GENBANK/EF065512 SI - GENBANK/EU265697 SI - GENBANK/HQ585081 SI - GENBANK/HQ585082 SI - GENBANK/JX537911 SI - GENBANK/KC154063 SI - GENBANK/KF294381 SI - GENBANK/KF294382 SI - GENBANK/KF294456 SI - GENBANK/KF430219 SI - GENBANK/KJ473821 SI - GENBANK/KU528584 SI - GENBANK/KU528594 PT - Journal Article DEP - 20160523 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 SB - IM MH - Animals MH - Chiroptera/*virology MH - Coronavirus/*isolation & purification MH - Feces/*virology MH - Republic of Korea MH - Rotavirus/*isolation & purification MH - Severe acute respiratory syndrome-related coronavirus/*isolation & purification PMC - PMC7169817 OTO - NOTNLM OT - Korea OT - Middle East respiratory syndrome OT - bat OT - coronavirus OT - group H rotavirus OT - severe acute respiratory syndrome EDAT- 2016/05/24 06:00 MHDA- 2017/01/20 06:00 PMCR- 2020/04/20 CRDT- 2016/05/24 06:00 PHST- 2016/02/02 00:00 [received] PHST- 2016/05/24 06:00 [entrez] PHST- 2016/05/24 06:00 [pubmed] PHST- 2017/01/20 06:00 [medline] PHST- 2020/04/20 00:00 [pmc-release] AID - TBED12515 [pii] AID - 10.1111/tbed.12515 [doi] PST - ppublish SO - Transbound Emerg Dis. 2016 Aug;63(4):365-72. doi: 10.1111/tbed.12515. Epub 2016 May 23. PMID- 30804542 OWN - NLM STAT- MEDLINE DCOM- 20190626 LR - 20220420 IS - 2058-5276 (Electronic) IS - 2058-5276 (Linking) VI - 4 IP - 5 DP - 2019 May TI - Dampened NLRP3-mediated inflammation in bats and implications for a special viral reservoir host. PG - 789-799 LID - 10.1038/s41564-019-0371-3 [doi] AB - Bats are special in their ability to host emerging viruses. As the only flying mammal, bats endure high metabolic rates yet exhibit elongated lifespans. It is currently unclear whether these unique features are interlinked. The important inflammasome sensor, NLR family pyrin domain containing 3 (NLRP3), has been linked to both viral-induced and age-related inflammation. Here, we report significantly dampened activation of the NLRP3 inflammasome in bat primary immune cells compared to human or mouse counterparts. Lower induction of apoptosis-associated speck-like protein containing a CARD (ASC) speck formation and secretion of interleukin-1β in response to both 'sterile' stimuli and infection with multiple zoonotic viruses including influenza A virus (-single-stranded (ss) RNA), Melaka virus (PRV3M, double-stranded RNA) and Middle East respiratory syndrome coronavirus (+ssRNA) was observed. Importantly, this reduction of inflammation had no impact on the overall viral loads. We identified dampened transcriptional priming, a novel splice variant and an altered leucine-rich repeat domain of bat NLRP3 as the cause. Our results elucidate an important mechanism through which bats dampen inflammation with implications for longevity and unique viral reservoir status. FAU - Ahn, Matae AU - Ahn M AUID- ORCID: 0000-0003-2114-8250 AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Anderson, Danielle E AU - Anderson DE AUID- ORCID: 0000-0003-4791-5024 AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Zhang, Qian AU - Zhang Q AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Tan, Chee Wah AU - Tan CW AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Lim, Beng Lee AU - Lim BL AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Luko, Katarina AU - Luko K AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Wen, Ming AU - Wen M AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Chia, Wan Ni AU - Chia WN AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Mani, Shailendra AU - Mani S AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Wang, Loo Chien AU - Wang LC AD - Functional Proteomics Laboratory, Institute of Molecular and Cellular Biology (A*STAR), Singapore, Singapore. FAU - Ng, Justin Han Jia AU - Ng JHJ AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. FAU - Sobota, Radoslaw M AU - Sobota RM AUID- ORCID: 0000-0002-2455-2526 AD - Functional Proteomics Laboratory, Institute of Molecular and Cellular Biology (A*STAR), Singapore, Singapore. AD - Institute of Medical Biology (IMB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. FAU - Dutertre, Charles-Antoine AU - Dutertre CA AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. AD - Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. FAU - Ginhoux, Florent AU - Ginhoux F AUID- ORCID: 0000-0002-2857-7755 AD - Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. FAU - Shi, Zheng-Li AU - Shi ZL AUID- ORCID: 0000-0001-8089-163X AD - CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Irving, Aaron T AU - Irving AT AUID- ORCID: 0000-0002-0196-1570 AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. aaron.irving@duke-nus.edu.sg. FAU - Wang, Lin-Fa AU - Wang LF AUID- ORCID: 0000-0003-2752-0535 AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore. linfa.wang@duke-nus.edu.sg. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190225 PL - England TA - Nat Microbiol JT - Nature microbiology JID - 101674869 RN - 0 (Inflammasomes) RN - 0 (Interleukin-1beta) RN - 0 (NLR Family, Pyrin Domain-Containing 3 Protein) SB - IM CIN - Nat Rev Microbiol. 2019 May;17(5):265. doi: 10.1038/s41579-019-0177-6. PMID: 30842614 CIN - Nat Microbiol. 2019 May;4(5):728-729. doi: 10.1038/s41564-019-0430-9. PMID: 31015739 MH - Animals MH - Chiroptera/genetics/*immunology/*virology MH - Coronavirus Infections/immunology/virology MH - Disease Reservoirs/*virology MH - Humans MH - Inflammasomes/chemistry/genetics/immunology MH - Influenza A virus/genetics/immunology MH - Influenza, Human/genetics/immunology/virology MH - Interleukin-1beta/genetics/immunology MH - Mice MH - Middle East Respiratory Syndrome Coronavirus/genetics/immunology MH - NLR Family, Pyrin Domain-Containing 3 Protein/chemistry/genetics/*immunology MH - Protein Domains PMC - PMC7096966 COIS- The authors declare no competing interests. EDAT- 2019/02/26 06:00 MHDA- 2019/06/27 06:00 PMCR- 2020/03/26 CRDT- 2019/02/27 06:00 PHST- 2018/08/14 00:00 [received] PHST- 2019/01/16 00:00 [accepted] PHST- 2019/02/26 06:00 [pubmed] PHST- 2019/06/27 06:00 [medline] PHST- 2019/02/27 06:00 [entrez] PHST- 2020/03/26 00:00 [pmc-release] AID - 10.1038/s41564-019-0371-3 [pii] AID - 371 [pii] AID - 10.1038/s41564-019-0371-3 [doi] PST - ppublish SO - Nat Microbiol. 2019 May;4(5):789-799. doi: 10.1038/s41564-019-0371-3. Epub 2019 Feb 25. PMID- 25514120 OWN - NLM STAT- MEDLINE DCOM- 20150826 LR - 20141217 IS - 1557-7759 (Electronic) IS - 1530-3667 (Linking) VI - 14 IP - 12 DP - 2014 Dec TI - Molecular survey of RNA viruses in Hungarian bats: discovering novel astroviruses, coronaviruses, and caliciviruses. PG - 846-55 LID - 10.1089/vbz.2014.1637 [doi] AB - Background: Bat-borne viruses pose a potential risk to human health and are the focus of increasing scientific interest. To start gaining information about bat-transmitted viruses in Hungary, we tested multiple bat species for several virus groups between 2012 and 2013. MATERIALS AND METHODS: Fecal samples were collected from bats across Hungary. We performed group-specific RT-PCR screening for astro-, calici-, corona-, lyssa-, othoreo-, paramyxo-, and rotaviruses. Positive samples were selected and sequenced for further phylogenetic analyses. RESULTS: A total of 447 fecal samples, representing 24 European bat species were tested. Novel strains of astroviruses, coronaviruses, and caliciviruses were detected and analyzed phylogenetically. Out of the 447 tested samples, 40 (9%) bats were positive for at least one virus. Bat-transmitted astroviruses (BtAstV) were detected in eight species with a 6.93% detection rate (95% confidence interval [CI] 4.854, 9.571). Coronaviruses (BtCoV) were detected in seven bat species with a detection rate of 1.79% (95% CI 0.849, 3.348), whereas novel caliciviruses (BtCalV) were detected in three bat species with a detection rate of 0.67% (95% CI 0.189, 1.780). Phylogenetic analyses revealed a great diversity among astrovirus strains, whereas the Hungarian BtCoV strains clustered together with both alpha- and betacoronavirus strains from other European countries. One of the most intriguing findings of our investigation is the discovery of novel BtCalVs in Europe. The Hungarian BtCalV did not cluster with any of the calcivirus genera identified in the family so far. CONCLUSIONS: We have successfully confirmed BtCoVs in numerous bat species. Furthermore, we have described new bat species harboring BtAstVs in Europe and found new species of CalVs. Further long-term investigations involving more species are needed in the Central European region for a better understanding on the host specificity, seasonality, phylogenetic relationships, and the possible zoonotic potential of these newly described viruses. FAU - Kemenesi, Gábor AU - Kemenesi G AD - 1 Virological Research Group, János Szentágothai Research Center, University of Pécs , Pécs, Hungary . FAU - Dallos, Bianka AU - Dallos B FAU - Görföl, Tamás AU - Görföl T FAU - Boldogh, Sándor AU - Boldogh S FAU - Estók, Péter AU - Estók P FAU - Kurucz, Kornélia AU - Kurucz K FAU - Kutas, Anna AU - Kutas A FAU - Földes, Fanni AU - Földes F FAU - Oldal, Miklós AU - Oldal M FAU - Németh, Viktória AU - Németh V FAU - Martella, Vito AU - Martella V FAU - Bányai, Krisztián AU - Bányai K FAU - Jakab, Ferenc AU - Jakab F LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Vector Borne Zoonotic Dis JT - Vector borne and zoonotic diseases (Larchmont, N.Y.) JID - 100965525 SB - IM MH - Animals MH - Astroviridae/classification/genetics/isolation & purification MH - Base Sequence MH - Caliciviridae/classification/genetics/isolation & purification MH - Chiroptera/*virology MH - Coronavirus/classification/genetics/isolation & purification MH - Hungary/epidemiology MH - Phylogeny MH - Prevalence MH - RNA Virus Infections/epidemiology/*veterinary/virology MH - RNA Viruses/*classification/*genetics/isolation & purification MH - Real-Time Polymerase Chain Reaction OTO - NOTNLM OT - Astroviruses OT - Bats OT - Calciviruses OT - Central Europe OT - Coronaviruses EDAT- 2014/12/17 06:00 MHDA- 2015/08/27 06:00 CRDT- 2014/12/17 06:00 PHST- 2014/12/17 06:00 [entrez] PHST- 2014/12/17 06:00 [pubmed] PHST- 2015/08/27 06:00 [medline] AID - 10.1089/vbz.2014.1637 [doi] PST - ppublish SO - Vector Borne Zoonotic Dis. 2014 Dec;14(12):846-55. doi: 10.1089/vbz.2014.1637. PMID- 26714453 OWN - NLM STAT- MEDLINE DCOM- 20160902 LR - 20181113 IS - 1746-6148 (Electronic) IS - 1746-6148 (Linking) VI - 11 DP - 2015 Dec 30 TI - First detection, clinical presentation and phylogenetic characterization of Porcine epidemic diarrhea virus in Austria. PG - 310 LID - 10.1186/s12917-015-0624-1 [doi] LID - 310 AB - BACKGROUND: Porcine epidemic diarrhea (PED) is a syndrome that is characterized by rapidly spreading watery diarrhea affecting pigs of all ages, but with major effects on suckling piglets. The disease, as well as the causative Alphacoronavirus, the Porcine epidemic diarrhea virus (PEDV), was first described in Europe in the 1970s and since then has spread over many Asian and American countries, where it recently led to devastating effects on swine health and pork industry. While the disease was seldom reported in Europe within the last few decades, a few recent reports re-emergence of PED in German pig farms. The hitherto isolated German strain seems to be closely related to a low pathogenic PEDV variant from the USA. This case report describes the first detection of PEDV in Austria. CASE PRESENTATION: Reduced feed uptake and occasional diarrhea were observed in December 2014 in a group of fattening pigs, kept on an Austrian swine farm. The concerned pigs had been recently purchased from Germany. Within a few weeks, diarrhea became apparent also in pigs of Austrian origin, which were kept in a different stable on the same farm. Gastrointestinal symptoms among fattening pigs were generally mild, quickly resolving and did not lead to death. PEDV RNA was identified by RT-qPCR in pooled feces and serum and PEDV antibodies were detectable in serum in both groups of pigs. Phylogenetic analysis of the nearly complete PEDV spike gene shows that the Austrian PEDV strain is highly similar to other strains involved in recent outbreaks in Western and Central Europe. CONCLUSION: This is the first report demonstrating the presence of PEDV in Austria. The virus was probably introduced by purchasing piglets from a German source, which underlines the significance of trans-boundary animal trade for the distribution of highly contagious diseases, such as PED. FAU - Steinrigl, Adolf AU - Steinrigl A AD - Austrian Agency for Health and Food Safety, Institute for Veterinary Disease Control, Robert Koch Gasse 17, 2340, Mödling, Austria. adolf.steinrigl@ages.at. FAU - Fernández, Sandra Revilla AU - Fernández SR AD - Austrian Agency for Health and Food Safety, Institute for Veterinary Disease Control, Robert Koch Gasse 17, 2340, Mödling, Austria. sandra.revilla-fernandez@ages.at. FAU - Stoiber, Friedrich AU - Stoiber F AD - Veterinary practice, Römerstraße 56, 4600, Wels, Austria. f.stoiber@a1.net. FAU - Pikalo, Jutta AU - Pikalo J AD - Austrian Agency for Health and Food Safety, Institute for Veterinary Disease Control, Robert Koch Gasse 17, 2340, Mödling, Austria. jutta.pikalo@ages.at. FAU - Sattler, Tatjana AU - Sattler T AD - Austrian Agency for Health and Food Safety, Institute for Veterinary Disease Control, Robert Koch Gasse 17, 2340, Mödling, Austria. tasat@vetmed.uni-leipzig.de. AD - University of Leipzig, Large Animal Clinic for Internal Medicine, An den Tierkliniken 11, 04103, Leipzig, Germany. tasat@vetmed.uni-leipzig.de. FAU - Schmoll, Friedrich AU - Schmoll F AD - Austrian Agency for Health and Food Safety, Institute for Veterinary Disease Control, Robert Koch Gasse 17, 2340, Mödling, Austria. friedrich.schmoll@ages.at. LA - eng SI - GENBANK/KT206204 SI - GENBANK/KT206205 SI - GENBANK/KT206206 PT - Journal Article DEP - 20151230 PL - England TA - BMC Vet Res JT - BMC veterinary research JID - 101249759 SB - IM EIN - BMC Vet Res. 2016 Feb 01;12:23. doi: 10.1186/s12917-016-0647-2. PMID: 26832742 MH - Animals MH - Austria/epidemiology MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Molecular Epidemiology MH - Porcine epidemic diarrhea virus/genetics/*isolation & purification MH - Swine PMC - PMC4696200 EDAT- 2015/12/31 06:00 MHDA- 2016/09/03 06:00 PMCR- 2015/12/30 CRDT- 2015/12/31 06:00 PHST- 2015/07/16 00:00 [received] PHST- 2015/12/22 00:00 [accepted] PHST- 2015/12/31 06:00 [entrez] PHST- 2015/12/31 06:00 [pubmed] PHST- 2016/09/03 06:00 [medline] PHST- 2015/12/30 00:00 [pmc-release] AID - 10.1186/s12917-015-0624-1 [pii] AID - 624 [pii] AID - 10.1186/s12917-015-0624-1 [doi] PST - epublish SO - BMC Vet Res. 2015 Dec 30;11:310. doi: 10.1186/s12917-015-0624-1. PMID- 30593837 OWN - NLM STAT- MEDLINE DCOM- 20190415 LR - 20211204 IS - 1879-0984 (Electronic) IS - 0166-0934 (Print) IS - 0166-0934 (Linking) VI - 265 DP - 2019 Mar TI - Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus. PG - 66-70 LID - S0166-0934(18)30414-2 [pii] LID - 10.1016/j.jviromet.2018.12.010 [doi] AB - Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus which was associated with severe diarrhea disease in pigs. SADS-CoV was first detected and identified as the causative agent of a devastating swine disease outbreak in southern China in 2017. Routine monitoring and early detection of the source of infection is therefore integral to the prevention and control of SADS-CoV infection. In this study, a SYBR green-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) technique was established for rapid detection and monitoring of this emerging virus. Specific primers were designed based on the conserved region within the M gene of the viral genome. The lowest detection limit of the RT-qPCR assay was 10 copies/μL. This assay was specific and had no cross-reaction with other 11 swine viruses. The positive rate of 84 clinical samples for the SYBR green-based RT-qPCR and the conventional RT-PCR was 73.81% (62/84) and 53.57% (45/84), respectively. These results demonstrated that the SYBR green-based RT-qPCR technique was an effectively diagnostic method with higher sensitivity than probe-based RT-qPCR and gel-based RT-PCR for detection and epidemiological investigations of SADS-CoV. CI - Copyright © 2018 Elsevier B.V. All rights reserved. FAU - Ma, Lei AU - Ma L AD - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. FAU - Zeng, Fanwen AU - Zeng F AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. FAU - Cong, Feng AU - Cong F AD - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. FAU - Huang, Bihong AU - Huang B AD - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. FAU - Huang, Ren AU - Huang R AD - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. FAU - Ma, Jingyun AU - Ma J AD - College of Animal Science, South China Agricultural University, Guangzhou, China. Electronic address: majy2400@scau.edu.cn. FAU - Guo, Pengju AU - Guo P AD - Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou, China. Electronic address: vetbio2016@hotmail.com. LA - eng PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181226 PL - Netherlands TA - J Virol Methods JT - Journal of virological methods JID - 8005839 RN - 0 (Benzothiazoles) RN - 0 (DNA Primers) RN - 0 (Diamines) RN - 0 (Organic Chemicals) RN - 0 (Quinolines) RN - 163795-75-3 (SYBR Green I) RN - Swine acute diarrhea syndrome coronavirus SB - IM MH - Alphacoronavirus/genetics/*isolation & purification MH - Animals MH - Benzothiazoles MH - China MH - Coronavirus Infections/diagnosis/*veterinary/virology MH - DNA Primers/genetics MH - Diamines MH - Organic Chemicals/*analysis MH - Quinolines MH - Real-Time Polymerase Chain Reaction/*methods MH - Reverse Transcriptase Polymerase Chain Reaction/*methods MH - Sensitivity and Specificity MH - Staining and Labeling/*methods MH - Swine MH - Swine Diseases/*diagnosis/virology MH - Time Factors PMC - PMC7113735 OTO - NOTNLM OT - Detection OT - Quantitative PCR OT - Swine acute diarrhea syndrome coronavirus (SADS-CoV) EDAT- 2018/12/30 06:00 MHDA- 2019/04/16 06:00 PMCR- 2018/12/26 CRDT- 2018/12/30 06:00 PHST- 2018/08/17 00:00 [received] PHST- 2018/11/05 00:00 [revised] PHST- 2018/12/17 00:00 [accepted] PHST- 2018/12/30 06:00 [pubmed] PHST- 2019/04/16 06:00 [medline] PHST- 2018/12/30 06:00 [entrez] PHST- 2018/12/26 00:00 [pmc-release] AID - S0166-0934(18)30414-2 [pii] AID - 10.1016/j.jviromet.2018.12.010 [doi] PST - ppublish SO - J Virol Methods. 2019 Mar;265:66-70. doi: 10.1016/j.jviromet.2018.12.010. Epub 2018 Dec 26. PMID- 30788861 OWN - NLM STAT- MEDLINE DCOM- 20190429 LR - 20231006 IS - 1939-1676 (Electronic) IS - 0891-6640 (Print) IS - 0891-6640 (Linking) VI - 33 IP - 2 DP - 2019 Mar TI - Evaluation of equine coronavirus fecal shedding among hospitalized horses. PG - 918-922 LID - 10.1111/jvim.15449 [doi] AB - BACKGROUND: Currently, diagnosis of equine coronavirus (ECoV) relies on the exclusion of other infectious causes of enteric disease along with molecular detection of ECoV in feces or tissue. Although this approach is complete, it is costly and may not always be achievable. OBJECTIVE: We hypothesized that the overall fecal shedding of ECoV in hospitalized horses is low. Our objective was to determine whether systemically healthy horses and horses with gastrointestinal disorders shed ECoV in their feces at the time of admission to a referral hospital and after 48 hours of stress associated with hospitalization. ANIMALS: One-hundred thirty adult horses admitted to the Washington State University Veterinary Teaching Hospital for gastrointestinal disease (n = 65) or for imaging under anesthesia (n = 65) that were hospitalized for 48 hours. Owner consent was obtained before sampling. METHODS: Fecal samples were collected at admission and 48 hours later. Polymerase chain reaction (PCR) for ECoV and electron microscopy (EM) were performed on all samples. RESULTS: Only 1 of 258 fecal samples was PCR-positive for ECoV. Electron microscopy identified ECoV-like particles in 9 of 258 samples, parvovirus-like particles in 4 of 258 samples, and rotavirus-like particles in 1 of 258 samples. CONCLUSIONS AND CLINICAL IMPORTANCE: The presence of ECoV in feces of hospitalized adult horses was low. Thus, fecal samples that are PCR-positive for ECoV in adult horses that have clinical signs consistent with this viral infection are likely to be of diagnostic relevance. The clinical relevance of the viruses observed using EM remains to be investigated. CI - © 2019 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine. FAU - Sanz, Macarena G AU - Sanz MG AUID- ORCID: 0000-0001-6565-9947 AD - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington. FAU - Kwon, SoYoung AU - Kwon S AD - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington. FAU - Pusterla, Nicola AU - Pusterla N AD - Department of Medicine and Epidemiology, University of California, Davis, California. FAU - Gold, Jenifer R AU - Gold JR AD - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington. FAU - Bain, Fairfield AU - Bain F AD - Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington. FAU - Evermann, Jim AU - Evermann J AD - Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, Washington. LA - eng GR - Boehringer Ingelheim/ PT - Journal Article DEP - 20190220 PL - United States TA - J Vet Intern Med JT - Journal of veterinary internal medicine JID - 8708660 SB - IM MH - Animals MH - Betacoronavirus 1/*isolation & purification MH - Feces/*microbiology/virology MH - Gastrointestinal Diseases/veterinary MH - Horse Diseases/*microbiology/virology MH - Horses MH - Hospitalization MH - Microscopy, Electron MH - Parvovirus/isolation & purification MH - Polymerase Chain Reaction/veterinary MH - Rotavirus/isolation & purification MH - Washington PMC - PMC6430884 OTO - NOTNLM OT - PCR OT - anesthesia OT - anorexia OT - electron microscopy OT - fever OT - gastrointestinal disease OT - lethargy COIS- Authors declare no conflict of interest. EDAT- 2019/02/23 06:00 MHDA- 2019/04/30 06:00 PMCR- 2019/03/01 CRDT- 2019/02/22 06:00 PHST- 2018/11/19 00:00 [received] PHST- 2019/01/29 00:00 [accepted] PHST- 2019/02/23 06:00 [pubmed] PHST- 2019/04/30 06:00 [medline] PHST- 2019/02/22 06:00 [entrez] PHST- 2019/03/01 00:00 [pmc-release] AID - JVIM15449 [pii] AID - 10.1111/jvim.15449 [doi] PST - ppublish SO - J Vet Intern Med. 2019 Mar;33(2):918-922. doi: 10.1111/jvim.15449. Epub 2019 Feb 20. PMID- 30646495 OWN - NLM STAT- MEDLINE DCOM- 20190516 LR - 20200309 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 11 IP - 1 DP - 2019 Jan 14 TI - Potent MERS-CoV Fusion Inhibitory Peptides Identified from HR2 Domain in Spike Protein of Bat Coronavirus HKU4. LID - 10.3390/v11010056 [doi] LID - 56 AB - The Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012 and caused continual outbreaks worldwide with high mortality. However, no effective anti-MERS-CoV drug is currently available. Recently, numerous evolutionary studies have suggested that MERS-CoV originated from bat coronavirus (BatCoV). We herein reported that three peptides derived from the HR2 region in spike protein of BatCoV HKU4, including HKU4-HR2P1, HKU4-HR2P2 and HKU4-HR2P3, could bind the MERS-CoV HR1-derived peptide to form a six-helix bundle (6-HB) with high stability. Moreover, these peptides, particularly HKU4-HR2P2 and HKU4-HR2P3, exhibited potent inhibitory activity against MERS-CoV S-mediated cell⁻cell fusion and viral infection, suggesting that these HKU4 HR2-derived peptides could be candidates for futher development as antiviral agents against MERS-CoV infection. FAU - Xia, Shuai AU - Xia S AUID- ORCID: 0000-0001-7896-4182 AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. 15111010053@fudan.edu.cn. FAU - Lan, Qiaoshuai AU - Lan Q AUID- ORCID: 0000-0001-5524-3542 AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. 18111010010@fudan.edu.cn. FAU - Pu, Jing AU - Pu J AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. 17111010015@fudan.edu.cn. FAU - Wang, Cong AU - Wang C AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. 16111010068@fudan.edu.cn. FAU - Liu, Zezhong AU - Liu Z AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. 17111010065@fudan.edu.cn. FAU - Xu, Wei AU - Xu W AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. xuwei11@fudan.edu.cn. FAU - Wang, Qian AU - Wang Q AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. wang_qian@fudan.edu.cn. FAU - Liu, Huan AU - Liu H AD - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. liuhuan@wh.iov.cn. FAU - Jiang, Shibo AU - Jiang S AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. shibojiang@fudan.edu.cn. AD - Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY 10065, USA. shibojiang@fudan.edu.cn. FAU - Lu, Lu AU - Lu L AUID- ORCID: 0000-0002-2255-0391 AD - Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan University, Shanghai 200032, China. lul@fudan.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190114 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Peptides) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Viral Fusion Proteins) SB - IM MH - Animals MH - Chiroptera/*virology MH - Middle East Respiratory Syndrome Coronavirus/*chemistry MH - Peptides/*chemistry MH - Phylogeny MH - Protein Binding MH - Spike Glycoprotein, Coronavirus/*chemistry MH - Viral Fusion Proteins/*chemistry MH - *Virus Internalization PMC - PMC6357153 OTO - NOTNLM OT - HKU4 OT - MERS-CoV OT - cell–cell fusion OT - fusion inhibitor OT - peptide COIS- The authors declare no conflicts of interest. The funding sponsors had no role in the writing of the manuscript or the decision to publish this article. EDAT- 2019/01/17 06:00 MHDA- 2019/05/17 06:00 PMCR- 2019/01/01 CRDT- 2019/01/17 06:00 PHST- 2018/12/07 00:00 [received] PHST- 2019/01/02 00:00 [revised] PHST- 2019/01/10 00:00 [accepted] PHST- 2019/01/17 06:00 [entrez] PHST- 2019/01/17 06:00 [pubmed] PHST- 2019/05/17 06:00 [medline] PHST- 2019/01/01 00:00 [pmc-release] AID - v11010056 [pii] AID - viruses-11-00056 [pii] AID - 10.3390/v11010056 [doi] PST - epublish SO - Viruses. 2019 Jan 14;11(1):56. doi: 10.3390/v11010056. PMID- 27030273 OWN - NLM STAT- MEDLINE DCOM- 20170503 LR - 20221207 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 90 IP - 12 DP - 2016 Jun 15 TI - Identification of the Fusion Peptide-Containing Region in Betacoronavirus Spike Glycoproteins. PG - 5586-5600 LID - 10.1128/JVI.00015-16 [doi] AB - The fusion peptides (FP) play an essential role in fusion of viral envelope with cellular membranes. The location and properties of the FPs in the spike (S) glycoproteins of different coronaviruses (CoV) have not yet been determined. Through amino acid sequence analysis of S proteins of representative CoVs, we identified a common region as a possible FP (pFP) that shares the characteristics of FPs of class I viral fusion proteins, including high Ala/Gly content, intermediate hydrophobicity, and few charged residues. To test the hypothesis that this region contains the CoV FP, we systemically mutated every residue in the pFP of Middle East respiratory syndrome betacoronavirus (MERS-CoV) and found that 11 of the 22 residues in the pFP (from G953 to L964, except for A956) were essential for S protein-mediated cell-cell fusion and virus entry. The synthetic MERS-CoV pFP core peptide (955IAGVGWTAGL964) induced extensive fusion of liposome membranes, while mutant peptide failed to induce any lipid mixing. We also selectively mutated residues in pFPs of two other β-CoVs, severe acute respiratory syndrome coronavirus (SARS-CoV) and mouse hepatitis virus (MHV). Although the amino acid sequences of these two pFPs differed significantly from that of MERS-CoV and each other, most of the pFP mutants of SARS-CoV and MHV also failed to mediate membrane fusion, suggesting that these pFPs are also the functional FPs. Thus, the FPs of 3 different lineages of β-CoVs are conserved in location within the S glycoproteins and in their functions, although their amino acid sequences have diverged significantly during CoV evolution. IMPORTANCE: Within the class I viral fusion proteins of many enveloped viruses, the FP is the critical mediator of fusion of the viral envelope with host cell membranes leading to virus infection. FPs from within a virus family, like influenza viruses or human immunodeficiency viruses (HIV), tend to share high amino acid sequence identity. In this study, we determined the location and amino acid sequences of the FPs of S glycoproteins of 3 β-CoVs, MERS-CoV, SARS-CoV, and MHV, and demonstrated that they were essential for mediating cell-cell fusion and virus entry. Interestingly, in marked contrast to the FPs of influenza and HIV, the primary amino acid sequences of the FPs of β-CoVs in 3 different lineages differed significantly. Thus, during evolution the FPs of β-CoVs have diverged significantly in their primary sequences while maintaining the same essential biological functions. Our findings identify a potential new target for development of drugs against CoVs. CI - Copyright © 2016, American Society for Microbiology. All Rights Reserved. FAU - Ou, Xiuyuan AU - Ou X AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. FAU - Zheng, Wangliang AU - Zheng W AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. FAU - Shan, Yiwei AU - Shan Y AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. FAU - Mu, Zhixia AU - Mu Z AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. FAU - Dominguez, Samuel R AU - Dominguez SR AD - Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, USA. FAU - Holmes, Kathryn V AU - Holmes KV AD - Department of Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA. FAU - Qian, Zhaohui AU - Qian Z AD - MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China zqian2013@sina.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160527 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Peptides) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Amino Acid Sequence MH - Animals MH - Evolution, Molecular MH - HEK293 Cells MH - Humans MH - Membrane Fusion MH - Mice MH - Middle East Respiratory Syndrome Coronavirus/*chemistry/genetics MH - Murine hepatitis virus/*chemistry/genetics MH - Mutation MH - Peptides/chemical synthesis/*chemistry/genetics MH - Severe acute respiratory syndrome-related coronavirus/*chemistry/genetics MH - Sequence Alignment MH - Spike Glycoprotein, Coronavirus/*chemistry/*genetics/metabolism MH - Virus Internalization PMC - PMC4886789 EDAT- 2016/04/01 06:00 MHDA- 2017/05/04 06:00 PMCR- 2016/11/27 CRDT- 2016/04/01 06:00 PHST- 2016/01/04 00:00 [received] PHST- 2016/03/23 00:00 [accepted] PHST- 2016/04/01 06:00 [entrez] PHST- 2016/04/01 06:00 [pubmed] PHST- 2017/05/04 06:00 [medline] PHST- 2016/11/27 00:00 [pmc-release] AID - JVI.00015-16 [pii] AID - 00015-16 [pii] AID - 10.1128/JVI.00015-16 [doi] PST - epublish SO - J Virol. 2016 May 27;90(12):5586-5600. doi: 10.1128/JVI.00015-16. Print 2016 Jun 15. PMID- 25525134 OWN - NLM STAT- MEDLINE DCOM- 20150914 LR - 20190522 IS - 1943-4936 (Electronic) IS - 1040-6387 (Linking) VI - 27 IP - 1 DP - 2015 Jan TI - Estimation of nasal shedding and seroprevalence of organisms known to be associated with bovine respiratory disease in Australian live export cattle. PG - 6-17 LID - 10.1177/1040638714559741 [doi] AB - The prevalence of organisms known to be associated with bovine respiratory disease (BRD) was investigated in cattle prior to export. A quantitative reverse transcription polymerase chain reaction assay was used to detect nucleic acids from the following viruses and bacteria in nasal swab samples: Bovine coronavirus (BoCV; Betacoronavirus 1), Bovine herpesvirus 1 (BoHV-1), Bovine viral diarrhea virus 1 (BVDV-1), Bovine respiratory syncytial virus (BRSV), Bovine parainfluenza virus 3 (BPIV-3), Histophilus somni, Mycoplasma bovis, Mannheimia haemolytica, and Pasteurella multocida. Between 2010 and 2012, nasal swabs were collected from 1,484 apparently healthy cattle destined for export to the Middle East and Russian Federation. In addition, whole blood samples from 334 animals were tested for antibodies to BoHV-1, BRSV, BVDV-1, and BPIV-3 using enzyme-linked immunosorbent assay. The nasal prevalence of BoCV at the individual animal level was 40.1%. The nasal and seroprevalence of BoHV-1, BRSV, BVDV-1, and BPIV-3 was 1.0% and 39%, 1.2% and 46%, 3.0% and 56%, and 1.4% and 87%, respectively. The nasal prevalence of H. somni, M. bovis, M. haemolytica, and P. multocida was 42%, 4.8%, 13.4%, and 26%, respectively. Significant differences in nasal and seroprevalence were detected between groups of animals from different geographical locations. The results of the current study provide baseline data on the prevalence of organisms associated with BRD in Australian live export cattle in the preassembly period. This data could be used to develop strategies for BRD prevention and control prior to loading. CI - © 2014 The Author(s). FAU - Moore, S Jo AU - Moore SJ AD - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia (Moore, O'Hara)Department of Agriculture and Food Western Australia, Australia (O'Dea)AusVet Animal Health Services, Toowoomba, Queensland, Australia (Perkins) j.moore@murdoch.edu.au. FAU - O'Dea, Mark A AU - O'Dea MA AD - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia (Moore, O'Hara)Department of Agriculture and Food Western Australia, Australia (O'Dea)AusVet Animal Health Services, Toowoomba, Queensland, Australia (Perkins). FAU - Perkins, Nigel AU - Perkins N AD - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia (Moore, O'Hara)Department of Agriculture and Food Western Australia, Australia (O'Dea)AusVet Animal Health Services, Toowoomba, Queensland, Australia (Perkins). FAU - O'Hara, Amanda J AU - O'Hara AJ AD - School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia (Moore, O'Hara)Department of Agriculture and Food Western Australia, Australia (O'Dea)AusVet Animal Health Services, Toowoomba, Queensland, Australia (Perkins). LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Vet Diagn Invest JT - Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc JID - 9011490 RN - 0 (Antibodies, Bacterial) RN - 0 (Antibodies, Viral) SB - IM MH - Animals MH - Antibodies, Bacterial/*blood MH - Antibodies, Viral/*blood MH - Australia/epidemiology MH - Bacteria/isolation & purification MH - Bacterial Shedding MH - Cattle MH - Cattle Diseases/*epidemiology/microbiology/virology MH - Enzyme-Linked Immunosorbent Assay/veterinary MH - Female MH - Male MH - Nose/microbiology/virology MH - Respiratory Tract Diseases/epidemiology/microbiology/*veterinary/virology MH - Seroepidemiologic Studies MH - Virus Shedding MH - Viruses/isolation & purification OTO - NOTNLM OT - Bovine coronavirus OT - Bovine herpesvirus 1 OT - Bovine parainfluenza virus 3 OT - Bovine respiratory syncytial virus OT - Bovine viral diarrhea virus OT - Histophilus somni OT - Mannheimia haemolytica OT - Mycoplasma bovis OT - Pasteurella multocida OT - bovine respiratory disease OT - cattle OT - live export EDAT- 2014/12/20 06:00 MHDA- 2015/09/15 06:00 CRDT- 2014/12/20 06:00 PHST- 2014/12/20 06:00 [entrez] PHST- 2014/12/20 06:00 [pubmed] PHST- 2015/09/15 06:00 [medline] AID - 27/1/6 [pii] AID - 10.1177/1040638714559741 [doi] PST - ppublish SO - J Vet Diagn Invest. 2015 Jan;27(1):6-17. doi: 10.1177/1040638714559741. PMID- 30541856 OWN - NLM STAT- MEDLINE DCOM- 20191120 LR - 20200309 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 93 IP - 5 DP - 2019 Mar 1 TI - Porcine Hemagglutinating Encephalomyelitis Virus Activation of the Integrin α5β1-FAK-Cofilin Pathway Causes Cytoskeletal Rearrangement To Promote Its Invasion of N2a Cells. LID - 10.1128/JVI.01736-18 [doi] LID - e01736-18 AB - Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurotropic virus that causes diffuse neuronal infection with neurological damage and high mortality. Virus-induced cytoskeletal dynamics are thought to be closely related to this type of nerve damage. Currently, the regulation pattern of the actin cytoskeleton and its molecular mechanism remain unclear when PHEV enters the host cells. Here, we demonstrate that entry of PHEV into N2a cells induces a biphasic remodeling of the actin cytoskeleton and a dynamic change in cofilin activity. Viral entry is affected by the disruption of actin kinetics or alteration of cofilin activity. PHEV binds to integrin α5β1 and then initiates the integrin α5β1-FAK signaling pathway, leading to virus-induced early cofilin phosphorylation and F-actin polymerization. Additionally, Ras-related C3 botulinum toxin substrate 1 (Rac1), cell division cycle 42 (Cdc42), and downstream regulatory gene p21-activated protein kinases (PAKs) are recruited as downstream mediators of PHEV-induced dynamic changes of the cofilin activity pathway. In conclusion, we demonstrate that PHEV utilizes the integrin α5β1-FAK-Rac1/Cdc42-PAK-LIMK-cofilin pathway to cause an actin cytoskeletal rearrangement to promote its own invasion, providing theoretical support for the development of PHEV pathogenic mechanisms and new antiviral targets.IMPORTANCE PHEV, a member of the Coronaviridae family, is a typical neurotropic virus that primarily affects the nervous system of piglets to produce typical neurological symptoms. However, the mechanism of nerve damage caused by the virus has not been fully elucidated. Actin is an important component of the cytoskeleton of eukaryotic cells and serves as the first obstacle to the entry of pathogens into host cells. Additionally, the morphological structure and function of nerve cells depend on the dynamic regulation of the actin skeleton. Therefore, exploring the mechanism of neuronal injury induced by PHEV from the perspective of the actin cytoskeleton not only helps elucidate the pathogenesis of PHEV but also provides a theoretical basis for the search for new antiviral targets. This is the first report to define a mechanistic link between alterations in signaling from cytoskeleton pathways and the mechanism of PHEV invading nerve cells. CI - Copyright © 2019 American Society for Microbiology. FAU - Lv, Xiaoling AU - Lv X AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Li, Zi AU - Li Z AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Guan, Jiyu AU - Guan J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Hu, Shiyu AU - Hu S AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Zhang, Jing AU - Zhang J AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lan, Yungang AU - Lan Y AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Zhao, Kui AU - Zhao K AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Lu, Huijun AU - Lu H AD - Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China. FAU - Song, Deguang AU - Song D AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - He, Hongbin AU - He H AD - College of Life Sciences, Shandong Normal University, Jinan, China. FAU - Gao, Feng AU - Gao F AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. FAU - He, Wenqi AU - He W AD - Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China hewq@jlu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190219 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Actin Depolymerizing Factors) RN - 0 (Integrin alpha5beta1) RN - EC 2.7.10.2 (Focal Adhesion Kinase 1) RN - EC 2.7.11.1 (p21-Activated Kinases) RN - EC 3.6.5.2 (cdc42 GTP-Binding Protein) SB - IM MH - Actin Cytoskeleton/*metabolism MH - Actin Depolymerizing Factors/*metabolism MH - Animals MH - Betacoronavirus 1/*metabolism MH - Cell Line MH - Coronavirus Infections/pathology MH - Focal Adhesion Kinase 1/*metabolism MH - Integrin alpha5beta1/*metabolism MH - Nerve Degeneration/*veterinary/virology MH - Swine MH - cdc42 GTP-Binding Protein/metabolism MH - p21-Activated Kinases/metabolism PMC - PMC6384086 OTO - NOTNLM OT - cofilin OT - coronavirus OT - cytoskeletal rearrangement OT - integrin α5β1 OT - neurotropic virus OT - porcine hemagglutinating encephalomyelitis virus EDAT- 2018/12/14 06:00 MHDA- 2019/11/21 06:00 PMCR- 2019/02/19 CRDT- 2018/12/14 06:00 PHST- 2018/10/02 00:00 [received] PHST- 2018/12/05 00:00 [accepted] PHST- 2018/12/14 06:00 [pubmed] PHST- 2019/11/21 06:00 [medline] PHST- 2018/12/14 06:00 [entrez] PHST- 2019/02/19 00:00 [pmc-release] AID - JVI.01736-18 [pii] AID - 01736-18 [pii] AID - 10.1128/JVI.01736-18 [doi] PST - epublish SO - J Virol. 2019 Feb 19;93(5):e01736-18. doi: 10.1128/JVI.01736-18. Print 2019 Mar 1. PMID- 28508214 OWN - NLM STAT- MEDLINE DCOM- 20180305 LR - 20200407 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 1602 DP - 2017 TI - Efficient Reverse Genetic Systems for Rapid Genetic Manipulation of Emergent and Preemergent Infectious Coronaviruses. PG - 59-81 LID - 10.1007/978-1-4939-6964-7_5 [doi] AB - Emergent and preemergent coronaviruses (CoVs) pose a global threat that requires immediate intervention. Rapid intervention necessitates the capacity to generate, grow, and genetically manipulate infectious CoVs in order to rapidly evaluate pathogenic mechanisms, host and tissue permissibility, and candidate antiviral therapeutic efficacy. CoVs encode the largest viral RNA genomes at about 28-32,000 nucleotides in length, and thereby complicate efficient engineering of the genome. Deconstructing the genome into manageable fragments affords the plasticity necessary to rapidly introduce targeted genetic changes in parallel and assort mutated fragments while maximizing genome stability over time. In this protocol we describe a well-developed reverse genetic platform strategy for CoVs that is comprised of partitioning the viral genome into 5-7 independent DNA fragments (depending on the CoV genome), each subcloned into a plasmid for increased stability and ease of genetic manipulation and amplification. Coronavirus genomes are conveniently partitioned by introducing type IIS or IIG restriction enzyme recognition sites that confer directional cloning. Since each restriction site leaves a unique overhang between adjoining fragments, reconstruction of the full-length genome can be achieved through a standard DNA ligation comprised of equal molar ratios of each fragment. Using this method, recombinant CoVs can be rapidly generated and used to investigate host range, gene function, pathogenesis, and candidate therapeutics for emerging and preemergent CoVs both in vitro and in vivo. FAU - Cockrell, Adam S AU - Cockrell AS AD - Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA. FAU - Beall, Anne AU - Beall A AD - Departments of Microbiology and Immunology, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA. FAU - Yount, Boyd AU - Yount B AD - Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA. FAU - Baric, Ralph AU - Baric R AD - Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA. rbaric@email.unc.edu. AD - Departments of Microbiology and Immunology, University of North Carolina-Chapel Hill, Chapel Hill, NC, USA. rbaric@email.unc.edu. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 0 (DNA, Complementary) RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Chlorocebus aethiops MH - Communicable Diseases, Emerging/transmission/virology MH - Coronavirus/*genetics MH - Coronavirus Infections/transmission/*virology MH - DNA, Complementary MH - Gene Expression Regulation, Viral MH - Genetic Engineering MH - Genome, Viral MH - Humans MH - Plasmids/genetics MH - RNA, Viral MH - Recombination, Genetic MH - *Reverse Genetics/methods MH - Transfection MH - Vero Cells PMC - PMC7120940 OTO - NOTNLM OT - Bat coronavirus OT - Coronavirus (CoV) OT - Emerging OT - Middle East respiratory syndrome coronavirus (MERS-CoV) OT - Porcine epidemic diarrhea virus (PEDV) OT - Preemergent OT - Reverse genetics OT - Severe acute respiratory syndrome coronavirus (SARS-CoV) EDAT- 2017/05/17 06:00 MHDA- 2018/03/06 06:00 PMCR- 2020/04/03 CRDT- 2017/05/17 06:00 PHST- 2017/05/17 06:00 [entrez] PHST- 2017/05/17 06:00 [pubmed] PHST- 2018/03/06 06:00 [medline] PHST- 2020/04/03 00:00 [pmc-release] AID - 5 [pii] AID - 10.1007/978-1-4939-6964-7_5 [doi] PST - ppublish SO - Methods Mol Biol. 2017;1602:59-81. doi: 10.1007/978-1-4939-6964-7_5. PMID- 27173881 OWN - NLM STAT- MEDLINE DCOM- 20180410 LR - 20240527 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 6 DP - 2016 May 13 TI - X-Ray Structure and Inhibition of 3C-like Protease from Porcine Epidemic Diarrhea Virus. PG - 25961 LID - 10.1038/srep25961 [doi] LID - 25961 AB - Porcine epidemic diarrhea virus (PEDV) is a coronavirus that infects pigs and can have mortality rates approaching 100% in piglets, causing serious economic impact. The 3C-like protease (3CL(pro)) is essential for the coronaviral life cycle and is an appealing target for the development of therapeutics. We report the expression, purification, crystallization and 2.10 Å X-ray structure of 3CL(pro) from PEDV. Analysis of the PEDV 3CL(pro) structure and comparison to other coronaviral 3CL(pro)'s from the same alpha-coronavirus phylogeny shows that the overall structures and active site architectures across 3CL(pro)'s are conserved, with the exception of a loop that comprises the protease S2 pocket. We found a known inhibitor of severe acute respiratory syndrome coronavirus (SARS-CoV) 3CL(pro), (R)-16, to have inhibitor activity against PEDV 3CL(pro), despite that SARS-3CL(pro) and PEDV 3CL(pro) share only 45.4% sequence identity. Structural comparison reveals that the majority of residues involved in (R)-16 binding to SARS-3CL(pro) are conserved in PEDV-3CL(pro); however, the sequence variation and positional difference in the loop forming the S2 pocket may account for large observed difference in IC50 values. This work advances our understanding of the subtle, but important, differences in coronaviral 3CL(pro) architecture and contributes to the broader structural knowledge of coronaviral 3CL(pro)'s. FAU - St John, Sarah E AU - St John SE AD - Department of Chemistry, Purdue University, West Lafayette, Indiana, USA. AD - Centers for Cancer Research &Drug Discovery, Purdue University, West Lafayette, Indiana, USA. AD - Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA. FAU - Anson, Brandon J AU - Anson BJ AD - Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA. FAU - Mesecar, Andrew D AU - Mesecar AD AD - Department of Chemistry, Purdue University, West Lafayette, Indiana, USA. AD - Centers for Cancer Research &Drug Discovery, Purdue University, West Lafayette, Indiana, USA. AD - Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA. LA - eng GR - P30 CA023168/CA/NCI NIH HHS/United States GR - R01 AI026603/AI/NIAID NIH HHS/United States GR - R01 AI085089/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20160513 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Viral Proteins) RN - EC 3.4.22.- (Cysteine Endopeptidases) SB - IM MH - Animals MH - Catalytic Domain MH - Crystallography, X-Ray MH - Cysteine Endopeptidases/*chemistry/*metabolism MH - Models, Molecular MH - Porcine epidemic diarrhea virus/chemistry/*enzymology MH - Protein Conformation MH - Structural Homology, Protein MH - Swine MH - Viral Proteins/*chemistry/*metabolism PMC - PMC4865815 EDAT- 2016/05/14 06:00 MHDA- 2018/04/11 06:00 PMCR- 2016/05/13 CRDT- 2016/05/14 06:00 PHST- 2016/02/11 00:00 [received] PHST- 2016/04/26 00:00 [accepted] PHST- 2016/05/14 06:00 [entrez] PHST- 2016/05/14 06:00 [pubmed] PHST- 2018/04/11 06:00 [medline] PHST- 2016/05/13 00:00 [pmc-release] AID - srep25961 [pii] AID - 10.1038/srep25961 [doi] PST - epublish SO - Sci Rep. 2016 May 13;6:25961. doi: 10.1038/srep25961. PMID- 27527760 OWN - NLM STAT- MEDLINE DCOM- 20170421 LR - 20200505 IS - 1873-2542 (Electronic) IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 192 DP - 2016 Aug 30 TI - Molecular surveillance of traditional and emerging pathogens associated with canine infectious respiratory disease. PG - 21-25 LID - S0378-1135(16)30164-X [pii] LID - 10.1016/j.vetmic.2016.06.009 [doi] AB - A molecular survey for traditional and emerging pathogens associated with canine infectious respiratory disease (CIRD) was conducted in Italy between 2011 and 2013 on a total of 138 dogs, including 78 early acute clinically ill CIRD animals, 22 non-clinical but exposed to clinically ill CIRD dogs and 38 CIRD convalescent dogs. The results showed that canine parainfluenza virus (CPIV) was the most commonly detected CIRD pathogen, followed by canine respiratory coronavirus (CRCoV), Bordetella bronchiseptica, Mycoplasma cynos, Mycoplasma canis and canine pneumovirus (CnPnV). Some classical CIRD agents, such as canine adenoviruses, canine distemper virus and canid herpesvirus 1, were not detected at all, as were not other emerging respiratory viruses (canine influenza virus, canine hepacivirus) and bacteria (Streptococcus equi subsp. zooepidemicus). Most severe forms of respiratory disease were observed in the presence of CPIV, CRCoV and M. cynos alone or in combination with other pathogens, whereas single CnPnV or M. canis infections were detected in dogs with no or very mild respiratory signs. Interestingly, only the association of M. cynos (alone or in combination with either CRCoV or M. canis) with severe clinical forms was statistically significant. The study, while confirming CPIV as the main responsible for CIRD occurrence, highlights the increasing role of recently discovered viruses, such as CRCoV and CnPnV, for which effective vaccines are not available in the market. CI - Copyright © 2016 Elsevier B.V. All rights reserved. FAU - Decaro, Nicola AU - Decaro N AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. Electronic address: nicola.decaro@uniba.it. FAU - Mari, Viviana AU - Mari V AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Larocca, Vittorio AU - Larocca V AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Losurdo, Michele AU - Losurdo M AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Lanave, Gianvito AU - Lanave G AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Lucente, Maria Stella AU - Lucente MS AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Corrente, Marialaura AU - Corrente M AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Catella, Cristiana AU - Catella C AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Bo, Stefano AU - Bo S AD - Veterinari Associati, Torino, Italy. FAU - Elia, Gabriella AU - Elia G AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Torre, Giorgio AU - Torre G AD - ASL BAT, Barletta, Italy. FAU - Grandolfo, Erika AU - Grandolfo E AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Martella, Vito AU - Martella V AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. FAU - Buonavoglia, Canio AU - Buonavoglia C AD - Department of Veterinary Medicine, University of Bari, Valenzano Bari, Italy. LA - eng PT - Journal Article DEP - 20160622 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 SB - IM MH - Animals MH - Bacterial Infections/epidemiology/microbiology/transmission/*veterinary MH - Coinfection MH - Communicable Diseases, Emerging/epidemiology/*veterinary MH - Dog Diseases/epidemiology/*microbiology/transmission MH - Dogs MH - Italy/epidemiology MH - *Molecular Epidemiology MH - Population Surveillance MH - Respiratory Tract Infections/epidemiology/transmission/*veterinary MH - Virus Diseases/epidemiology/transmission/*veterinary/virology PMC - PMC7131703 OTO - NOTNLM OT - Dog OT - Molecular survey OT - Pathogens OT - Respiratory disease EDAT- 2016/08/17 06:00 MHDA- 2017/04/22 06:00 PMCR- 2016/06/22 CRDT- 2016/08/17 06:00 PHST- 2016/03/22 00:00 [received] PHST- 2016/06/08 00:00 [revised] PHST- 2016/06/21 00:00 [accepted] PHST- 2016/08/17 06:00 [entrez] PHST- 2016/08/17 06:00 [pubmed] PHST- 2017/04/22 06:00 [medline] PHST- 2016/06/22 00:00 [pmc-release] AID - S0378-1135(16)30164-X [pii] AID - 10.1016/j.vetmic.2016.06.009 [doi] PST - ppublish SO - Vet Microbiol. 2016 Aug 30;192:21-25. doi: 10.1016/j.vetmic.2016.06.009. Epub 2016 Jun 22. PMID- 27617430 OWN - NLM STAT- MEDLINE DCOM- 20170508 LR - 20210514 IS - 1545-9985 (Electronic) IS - 1545-9993 (Print) IS - 1545-9985 (Linking) VI - 23 IP - 10 DP - 2016 Oct TI - Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy. PG - 899-905 LID - 10.1038/nsmb.3293 [doi] AB - The threat of a major coronavirus pandemic urges the development of strategies to combat these pathogens. Human coronavirus NL63 (HCoV-NL63) is an α-coronavirus that can cause severe lower-respiratory-tract infections requiring hospitalization. We report here the 3.4-Å-resolution cryo-EM reconstruction of the HCoV-NL63 coronavirus spike glycoprotein trimer, which mediates entry into host cells and is the main target of neutralizing antibodies during infection. The map resolves the extensive glycan shield obstructing the protein surface and, in combination with mass spectrometry, provides a structural framework to understand the accessibility to antibodies. The structure reveals the complete architecture of the fusion machinery including the triggering loop and the C-terminal domains, which contribute to anchoring the trimer to the viral membrane. Our data further suggest that HCoV-NL63 and other coronaviruses use molecular trickery, based on epitope masking with glycans and activating conformational changes, to evade the immune system of infected hosts. FAU - Walls, Alexandra C AU - Walls AC AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA. FAU - Tortorici, M Alejandra AU - Tortorici MA AD - Institut Pasteur, Unité de Virologie Structurale, Paris, France. AD - CNRS UMR 3569 Virologie, Paris, France. FAU - Frenz, Brandon AU - Frenz B AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA. FAU - Snijder, Joost AU - Snijder J AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA. FAU - Li, Wentao AU - Li W AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. FAU - Rey, Félix A AU - Rey FA AD - Institut Pasteur, Unité de Virologie Structurale, Paris, France. AD - CNRS UMR 3569 Virologie, Paris, France. FAU - DiMaio, Frank AU - DiMaio F AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA. FAU - Bosch, Berend-Jan AU - Bosch BJ AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. FAU - Veesler, David AU - Veesler D AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA. LA - eng GR - R01 GM120553/GM/NIGMS NIH HHS/United States GR - S10 OD018111/OD/NIH HHS/United States GR - S10 RR023057/RR/NCRR NIH HHS/United States GR - T32 GM008268/GM/NIGMS NIH HHS/United States PT - Journal Article DEP - 20160912 PL - United States TA - Nat Struct Mol Biol JT - Nature structural & molecular biology JID - 101186374 RN - 0 (Antibodies, Neutralizing) RN - 0 (Epitopes) RN - 0 (Polysaccharides) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Antibodies, Neutralizing/immunology MH - Cell Line MH - Coronavirus Infections/immunology/*virology MH - Coronavirus NL63, Human/*chemistry/immunology MH - Cryoelectron Microscopy MH - Drosophila MH - Epitopes/*chemistry/immunology MH - Humans MH - Models, Molecular MH - Polysaccharides/*analysis/immunology MH - Protein Conformation MH - Protein Multimerization MH - Spike Glycoprotein, Coronavirus/*chemistry/immunology/ultrastructure PMC - PMC5515730 MID - NIHMS879468 COIS- The authors declare no competing financial interests. EDAT- 2016/09/13 06:00 MHDA- 2017/05/10 06:00 PMCR- 2020/03/26 CRDT- 2016/09/13 06:00 PHST- 2016/06/17 00:00 [received] PHST- 2016/08/17 00:00 [accepted] PHST- 2016/09/13 06:00 [pubmed] PHST- 2017/05/10 06:00 [medline] PHST- 2016/09/13 06:00 [entrez] PHST- 2020/03/26 00:00 [pmc-release] AID - nsmb.3293 [pii] AID - BFnsmb3293 [pii] AID - 10.1038/nsmb.3293 [doi] PST - ppublish SO - Nat Struct Mol Biol. 2016 Oct;23(10):899-905. doi: 10.1038/nsmb.3293. Epub 2016 Sep 12. PMID- 29801460 OWN - NLM STAT- MEDLINE DCOM- 20180926 LR - 20181114 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 15 IP - 1 DP - 2018 May 25 TI - Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States. PG - 95 LID - 10.1186/s12985-018-1001-z [doi] LID - 95 AB - BACKGROUND: Emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) in North America, Asia and Europe has caused severe economic loss to the global swine industry. However, the virome of PEDV infected pigs and its effect on disease severity remains unknown. The advancements of sequencing technology have made it possible to characterize the entire microbiome of different body sites for any host. METHODS: The objective of this study was to characterize the RNA virome in PEDV-positive pigs using the hypothesis-free metagenomics approach based on next-generation sequencing. Specifically, 217 PEDV-positive swine fecal swab samples collected from diarrheic piglets over 17 US states during 2015-2016 were analyzed. RESULTS: A Kraken algorithm-based bioinformatics analysis revealed the presence of up to 9 different RNA genera besides PEDV (Alphacoronavirus genus), including Mamastrovirus (52%, 113/217), Enterovirus (39%, 85/217), Sapelovirus (31%, 67/217), Posavirus (30%, 66/217), Kobuvirus (23%, 49/217), Sapovirus (13%, 28/217), Teschovirus (10%, 22/217), Pasivirus (9%, 20/217), and Deltacoronavirus (3%, 6/217). There were 58 out of 217 piglets (27%) have PEDV infection alone whereas the remaining 159 (73%) shed 2 up to 9 different viruses. CONCLUSION: These findings demonstrated that PEDV infected diarrheic pigs had an extensive RNA viral flora consisting of four different families: Astroviridae, Picornaviridae, Caliciviridae, and Coronaviridae. FAU - Chen, Qi AU - Chen Q AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. FAU - Wang, Leyi AU - Wang L AD - Department of Veterinary Clinical Medicine and the Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, IL, 61802, USA. FAU - Zheng, Ying AU - Zheng Y AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. FAU - Zhang, Jianqiang AU - Zhang J AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. FAU - Guo, Baoqing AU - Guo B AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. FAU - Yoon, Kyoung-Jin AU - Yoon KJ AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. FAU - Gauger, Phillip C AU - Gauger PC AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. FAU - Harmon, Karen M AU - Harmon KM AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. FAU - Main, Rodger G AU - Main RG AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. FAU - Li, Ganwu AU - Li G AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1907 ISU C-Drive, VMRI#1, Ames, IA, 50011, USA. liganwu@iastate.edu. LA - eng GR - NA/USDA NAHLN Program Office/International PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20180525 PL - England TA - Virol J JT - Virology journal JID - 101231645 RN - 0 (RNA, Viral) SB - IM MH - Algorithms MH - Amino Acid Sequence MH - Animals MH - Astroviridae/classification/*genetics/isolation & purification MH - Caliciviridae/classification/*genetics/isolation & purification MH - Coinfection MH - Computational Biology MH - Coronaviridae/classification/*genetics/isolation & purification MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Farms MH - Feces/virology MH - High-Throughput Nucleotide Sequencing MH - Metagenomics/methods MH - Phylogeny MH - Picornaviridae/classification/*genetics/isolation & purification MH - Porcine epidemic diarrhea virus/classification/*genetics/isolation & purification MH - RNA, Viral/genetics MH - Sequence Alignment MH - Sequence Analysis, DNA MH - Sequence Homology, Amino Acid MH - Swine MH - Swine Diseases/*epidemiology/virology MH - United States/epidemiology PMC - PMC5970503 OTO - NOTNLM OT - Coinfection OT - Metagenomic analysis OT - PEDV OT - RNA virome COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/05/29 06:00 MHDA- 2018/09/27 06:00 PMCR- 2018/05/25 CRDT- 2018/05/27 06:00 PHST- 2017/11/22 00:00 [received] PHST- 2018/05/13 00:00 [accepted] PHST- 2018/05/27 06:00 [entrez] PHST- 2018/05/29 06:00 [pubmed] PHST- 2018/09/27 06:00 [medline] PHST- 2018/05/25 00:00 [pmc-release] AID - 10.1186/s12985-018-1001-z [pii] AID - 1001 [pii] AID - 10.1186/s12985-018-1001-z [doi] PST - epublish SO - Virol J. 2018 May 25;15(1):95. doi: 10.1186/s12985-018-1001-z. PMID- 26404138 OWN - NLM STAT- MEDLINE DCOM- 20160819 LR - 20181113 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 5 DP - 2015 Sep 25 TI - The heptad repeat region is a major selection target in MERS-CoV and related coronaviruses. PG - 14480 LID - 10.1038/srep14480 [doi] LID - 14480 AB - Middle East respiratory syndrome coronavirus (MERS-CoV) originated in bats and spread to humans via zoonotic transmission from camels. We analyzed the evolution of the spike (S) gene in betacoronaviruses (betaCoVs) isolated from different mammals, in bat coronavirus populations, as well as in MERS-CoV strains from the current outbreak. Results indicated several positively selected sites located in the region comprising the two heptad repeats (HR1 and HR2) and their linker. Two sites (R652 and V1060) were positively selected in the betaCoVs phylogeny and correspond to mutations associated with expanded host range in other coronaviruses. During the most recent evolution of MERS-CoV, adaptive mutations in the HR1 (Q/R/H1020) arose in camels or in a previous host and spread to humans. We determined that different residues at position 1020 establish distinct inter- and intra-helical interactions and affect the stability of the six-helix bundle formed by the HRs. A similar effect on stability was observed for a nearby mutation (T1015N) that increases MERS-CoV infection efficiency in vitro. Data herein indicate that the heptad repeat region was a major target of adaptive evolution in MERS-CoV-related viruses; these results are relevant for the design of fusion inhibitor peptides with antiviral function. FAU - Forni, Diego AU - Forni D AD - Scientific Institute IRCCS E. MEDEA, Bioinformatics, 23842 Bosisio Parini, Italy. FAU - Filippi, Giulia AU - Filippi G AD - Department of Biotechnology and Biosciences, University of Milan-Bicocca, 20126 Milan, Italy. FAU - Cagliani, Rachele AU - Cagliani R AD - Scientific Institute IRCCS E. MEDEA, Bioinformatics, 23842 Bosisio Parini, Italy. FAU - De Gioia, Luca AU - De Gioia L AD - Department of Biotechnology and Biosciences, University of Milan-Bicocca, 20126 Milan, Italy. FAU - Pozzoli, Uberto AU - Pozzoli U AD - Scientific Institute IRCCS E. MEDEA, Bioinformatics, 23842 Bosisio Parini, Italy. FAU - Al-Daghri, Nasser AU - Al-Daghri N AD - Biomarkers research program, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia (KSA). AD - Prince Mutaib Chair for Biomarkers of Osteoporosis, Biochemistry Department, College of science, King Saud University, Riyadh, KSA. FAU - Clerici, Mario AU - Clerici M AD - Department of Physiopathology and Transplantation, University of Milan, 20090 Milan, Italy. AD - Don C. Gnocchi Foundation ONLUS, IRCCS, 20148 Milan, Italy. FAU - Sironi, Manuela AU - Sironi M AD - Scientific Institute IRCCS E. MEDEA, Bioinformatics, 23842 Bosisio Parini, Italy. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150925 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 SB - IM MH - Amino Acid Sequence MH - Animals MH - Coronavirus/*genetics MH - *Evolution, Molecular MH - Genes, Viral MH - Genetic Variation MH - Genotype MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*genetics MH - Molecular Sequence Data MH - Phylogeny MH - Recombination, Genetic MH - Repetitive Sequences, Nucleic Acid MH - *Selection, Genetic MH - Sequence Alignment PMC - PMC4585914 EDAT- 2015/09/26 06:00 MHDA- 2016/08/20 06:00 PMCR- 2015/09/25 CRDT- 2015/09/26 06:00 PHST- 2015/05/16 00:00 [received] PHST- 2015/09/01 00:00 [accepted] PHST- 2015/09/26 06:00 [entrez] PHST- 2015/09/26 06:00 [pubmed] PHST- 2016/08/20 06:00 [medline] PHST- 2015/09/25 00:00 [pmc-release] AID - srep14480 [pii] AID - 10.1038/srep14480 [doi] PST - epublish SO - Sci Rep. 2015 Sep 25;5:14480. doi: 10.1038/srep14480. PMID- 29093093 OWN - NLM STAT- MEDLINE DCOM- 20190903 LR - 20210323 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 92 IP - 4 DP - 2018 Feb 15 TI - Glycan Shield and Fusion Activation of a Deltacoronavirus Spike Glycoprotein Fine-Tuned for Enteric Infections. LID - 10.1128/JVI.01628-17 [doi] LID - e01628-17 AB - Coronaviruses recently emerged as major human pathogens causing outbreaks of severe acute respiratory syndrome and Middle East respiratory syndrome. They utilize the spike (S) glycoprotein anchored in the viral envelope to mediate host attachment and fusion of the viral and cellular membranes to initiate infection. The S protein is a major determinant of the zoonotic potential of coronaviruses and is also the main target of the host humoral immune response. We report here the 3.5-Å-resolution cryo-electron microscopy structure of the S glycoprotein trimer from the pathogenic porcine deltacoronavirus (PDCoV), which belongs to the recently identified Deltacoronavirus genus. Structural and glycoproteomics data indicate that the glycans of PDCoV S are topologically conserved compared with the human respiratory coronavirus NL63 S, resulting in similar surface areas being shielded from neutralizing antibodies and implying that both viruses are under comparable immune pressure in their respective hosts. The structure further reveals a shortened S(2)' activation loop, containing a reduced number of basic amino acids, which participates in rendering the spike largely protease resistant. This property distinguishes PDCoV S from recently characterized betacoronavirus S proteins and suggests that the S protein of enterotropic PDCoV has evolved to tolerate the protease-rich environment of the small intestine and to fine-tune its fusion activation to avoid premature triggering and reduction of infectivity.IMPORTANCE Coronaviruses use transmembrane S glycoprotein trimers to promote host attachment and fusion of the viral and cellular membranes. We determined a near-atomic-resolution cryo-electron microscopy structure of the S ectodomain trimer from the pathogenic PDCoV, which is responsible for diarrhea in piglets and has had devastating consequences for the swine industry worldwide. Structural and glycoproteomics data reveal that PDCoV S is decorated with 78 N-linked glycans obstructing the protein surface to limit accessibility to neutralizing antibodies in a way reminiscent of what has recently been described for a human respiratory coronavirus. PDCoV S is largely protease resistant, which distinguishes it from most other characterized coronavirus S glycoproteins and suggests that enteric coronaviruses have evolved to fine-tune fusion activation in the protease-rich environment of the small intestine of infected hosts. CI - Copyright © 2018 American Society for Microbiology. FAU - Xiong, Xiaoli AU - Xiong X AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA. FAU - Tortorici, M Alejandra AU - Tortorici MA AD - Institut Pasteur, Unité de Virologie Structurale, Paris, France. AD - CNRS UMR 3569 Virologie, Paris, France. FAU - Snijder, Joost AU - Snijder J AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA. FAU - Yoshioka, Craig AU - Yoshioka C AD - Department of Biomedical Engineering, Oregon Health and Science University, Portland, Oregon, USA. FAU - Walls, Alexandra C AU - Walls AC AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA. FAU - Li, Wentao AU - Li W AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. FAU - McGuire, Andrew T AU - McGuire AT AD - Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA. FAU - Rey, Félix A AU - Rey FA AD - Institut Pasteur, Unité de Virologie Structurale, Paris, France. AD - CNRS UMR 3569 Virologie, Paris, France. FAU - Bosch, Berend-Jan AU - Bosch BJ AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. FAU - Veesler, David AU - Veesler D AD - Department of Biochemistry, University of Washington, Seattle, Washington, USA dveesler@uw.edu. LA - eng GR - HHSN272201700059C/AI/NIAID NIH HHS/United States GR - R01 GM120553/GM/NIGMS NIH HHS/United States GR - T32 GM008268/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20180130 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Polysaccharides) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM CIN - https://doi.org/10.1128/JVI.01556-17 MH - Animals MH - Coronaviridae/classification/*physiology MH - Coronaviridae Infections/metabolism/*virology MH - Drosophila melanogaster/metabolism/virology MH - Humans MH - *Membrane Fusion MH - Polysaccharides/chemistry/*metabolism MH - Protein Conformation MH - Spike Glycoprotein, Coronavirus/*chemistry/*metabolism MH - Swine MH - Swine Diseases/pathology/virology MH - *Virus Attachment PMC - PMC5790929 OTO - NOTNLM OT - coronaviruses OT - cryo-EM OT - fusion proteins EDAT- 2017/11/03 06:00 MHDA- 2019/09/04 06:00 PMCR- 2018/07/30 CRDT- 2017/11/03 06:00 PHST- 2017/09/14 00:00 [received] PHST- 2017/10/23 00:00 [accepted] PHST- 2017/11/03 06:00 [pubmed] PHST- 2019/09/04 06:00 [medline] PHST- 2017/11/03 06:00 [entrez] PHST- 2018/07/30 00:00 [pmc-release] AID - JVI.01628-17 [pii] AID - 01628-17 [pii] AID - 10.1128/JVI.01628-17 [doi] PST - epublish SO - J Virol. 2018 Jan 30;92(4):e01628-17. doi: 10.1128/JVI.01628-17. Print 2018 Feb 15. PMID- 30447246 OWN - NLM STAT- MEDLINE DCOM- 20190122 LR - 20210109 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 260 DP - 2019 Jan 15 TI - Full genome characterization of two novel Alpha-coronavirus species from Italian bats. PG - 60-66 LID - S0168-1702(18)30585-9 [pii] LID - 10.1016/j.virusres.2018.11.007 [doi] AB - Coronaviruses (CoVs) have been detected worldwide in several bat species, which are considered the main reservoir. The attention to the high diversity of CoVs hosted by bats has increased during the last decade due to the high number of human infections caused by two zoonotic Beta-CoVs, SARS-CoV and MERS-CoV, that cause several respiratory diseases. Among coronaviruses, two Alpha-CoV strains (HuCoV-229E and HuCoV-NL63) cause mild respiratory disease that can change to severe disease in children, elderly and individuals affected by illnesses. Phylogenetic analysis conducted on bat Alpha-CoV strains revealed their evolutive correlation to human strains, suggesting their origin in bats. The genome of CoVs is characterized by a high frequency of mutations and recombination events, increasing their ability to switch hosts and their zoonotic potential. In this study, three strains of Alpha-CoV genera detected in Italian bats (Pipistrellus kuhlii) were fully sequenced by Next Generation Sequencing (NGS) and characterized. The complete genome analysis showed the correlation of the Italians strains with a Chinese strain detected in 2013 and, based on CoV molecular species demarcation, two new Alpha-CoV species were established. The analysis of a fragment of the RNA-dependent RNA polymerase (RdRp) showed the correlation of the Italian strains with CoVs that was only detected in the bat Pipistrellus genera (Pipistrellus kuhlii and Pipistrellus Pipistrellus) in European countries. CI - Copyright © 2018 Elsevier B.V. All rights reserved. FAU - De Sabato, Luca AU - De Sabato L AD - Department of Sciences, University Roma Tre, Viale Guglielmo Marconi 446, 00146, Rome, Italy; Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy. FAU - Lelli, Davide AU - Lelli D AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. FAU - Faccin, Francesca AU - Faccin F AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. FAU - Canziani, Sabrina AU - Canziani S AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. FAU - Di Bartolo, Ilaria AU - Di Bartolo I AD - Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy. FAU - Vaccari, Gabriele AU - Vaccari G AD - Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy. Electronic address: gabriele.vaccari@iss.it. FAU - Moreno, Ana AU - Moreno A AD - Department of Virology, Istituto Zooprofilattico Sperimentale Lombardia ed Emilia Romagna, Via Antonio Bianchi 9, 25124, Brescia, Italy. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181115 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 SB - IM MH - Animals MH - Chiroptera/*virology MH - Computational Biology MH - Coronavirus/classification/*genetics/*isolation & purification MH - Coronavirus Infections/*veterinary/virology MH - *Genome, Viral MH - High-Throughput Nucleotide Sequencing MH - Italy MH - Phylogeny MH - Sequence Homology PMC - PMC7114869 OTO - NOTNLM OT - Alpha-CoV viruses OT - Bats OT - Full genome sequencing OT - Italy EDAT- 2018/11/18 06:00 MHDA- 2019/01/23 06:00 PMCR- 2018/11/15 CRDT- 2018/11/18 06:00 PHST- 2018/09/25 00:00 [received] PHST- 2018/11/07 00:00 [revised] PHST- 2018/11/13 00:00 [accepted] PHST- 2018/11/18 06:00 [pubmed] PHST- 2019/01/23 06:00 [medline] PHST- 2018/11/18 06:00 [entrez] PHST- 2018/11/15 00:00 [pmc-release] AID - S0168-1702(18)30585-9 [pii] AID - 10.1016/j.virusres.2018.11.007 [doi] PST - ppublish SO - Virus Res. 2019 Jan 15;260:60-66. doi: 10.1016/j.virusres.2018.11.007. Epub 2018 Nov 15. PMID- 28393313 OWN - NLM STAT- MEDLINE DCOM- 20170823 LR - 20190822 IS - 1995-820X (Electronic) IS - 1674-0769 (Print) IS - 1995-820X (Linking) VI - 32 IP - 2 DP - 2017 Apr TI - Molecular detection of viruses in Kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses. PG - 101-114 LID - 10.1007/s12250-016-3930-2 [doi] AB - This is the first country-wide surveillance of bat-borne viruses in Kenya spanning from 2012-2015 covering sites perceived to have medium to high level bat-human interaction. The objective of this surveillance study was to apply a non-invasive approach using fresh feces to detect viruses circulating within the diverse species of Kenyan bats. We screened for both DNA and RNA viruses; specifically, astroviruses (AstVs), adenoviruses (ADVs), caliciviruses (CalVs), coronaviruses (CoVs), flaviviruses, filoviruses, paramyxoviruses (PMVs), polyomaviruses (PYVs) and rotaviruses. We used family-specific primers, amplicon sequencing and further characterization by phylogenetic analysis. Except for filoviruses, eight virus families were detected with varying distributions and positive rates across the five regions (former provinces) studied. AstVs (12.83%), CoVs (3.97%), PMV (2.4%), ADV (2.26%), PYV (1.65%), CalVs (0.29%), rotavirus (0.19%) and flavivirus (0.19%). Novel CalVs were detected in Rousettus aegyptiacus and Mops condylurus while novel Rotavirus-A-related viruses were detected in Taphozous bats and R. aegyptiacus. The two Rotavirus A (RVA) strains detected were highly related to human strains with VP6 genotypes I2 and I16. Genotype I16 has previously been assigned to human RVA-strain B10 from Kenya only, which raises public health concern, particularly considering increased human-bat interaction. Additionally, 229E-like bat CoVs were detected in samples originating from Hipposideros bats roosting in sites with high human activity. Our findings confirm the presence of diverse viruses in Kenyan bats while providing extended knowledge on bat virus distribution. The detection of viruses highly related to human strains and hence of public health concern, underscores the importance of continuous surveillance. FAU - Waruhiu, Cecilia AU - Waruhiu C AD - Key Lab of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Ommeh, Sheila AU - Ommeh S AD - Institute of Biotechnology Research, Jomo Kenyatta University of Science and Technology, Nairobi, 00200, Kenya. sommeh@jkuat.ac.ke. FAU - Obanda, Vincent AU - Obanda V AD - Veterinary Services Department, Kenya Wildlife Service, Nairobi, 00100, Kenya. FAU - Agwanda, Bernard AU - Agwanda B AD - Mammalogy Section, National Museum of Kenya, Nairobi, 00100, Kenya. FAU - Gakuya, Francis AU - Gakuya F AD - Veterinary Services Department, Kenya Wildlife Service, Nairobi, 00100, Kenya. FAU - Ge, Xing-Yi AU - Ge XY AD - Key Lab of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Yang, Xing-Lou AU - Yang XL AD - Key Lab of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Wu, Li-Jun AU - Wu LJ AD - Key Lab of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Zohaib, Ali AU - Zohaib A AD - Key Lab of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Hu, Ben AU - Hu B AD - Key Lab of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. AD - Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430071, China. FAU - Shi, Zheng-Li AU - Shi ZL AD - Key Lab of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn. AD - Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430071, China. zlshi@wh.iov.cn. LA - eng PT - Journal Article DEP - 20170406 PL - Netherlands TA - Virol Sin JT - Virologica Sinica JID - 101514185 SB - IM EIN - Virol Sin. 2018 Jun;33(3):294. doi: 10.1007/s12250-018-0035-0. PMID: 29808439 MH - Animals MH - Chiroptera/*virology MH - DNA Virus Infections/*veterinary/virology MH - DNA Viruses/classification/genetics/*isolation & purification MH - Feces/*virology MH - Kenya MH - Phylogeny MH - Polymerase Chain Reaction MH - RNA Virus Infections/*veterinary/virology MH - RNA Viruses/classification/genetics/*isolation & purification MH - Sequence Analysis, DNA PMC - PMC6702250 OTO - NOTNLM OT - 229-E-like bat coronavirus OT - Rotavirus A OT - astroviruses (AstVs) OT - calicivirus (CalVs) EDAT- 2017/04/11 06:00 MHDA- 2017/08/24 06:00 PMCR- 2017/04/06 CRDT- 2017/04/11 06:00 PHST- 2016/12/09 00:00 [received] PHST- 2017/02/15 00:00 [accepted] PHST- 2017/04/11 06:00 [pubmed] PHST- 2017/08/24 06:00 [medline] PHST- 2017/04/11 06:00 [entrez] PHST- 2017/04/06 00:00 [pmc-release] AID - 10.1007/s12250-016-3930-2 [pii] AID - 3930 [pii] AID - 10.1007/s12250-016-3930-2 [doi] PST - ppublish SO - Virol Sin. 2017 Apr;32(2):101-114. doi: 10.1007/s12250-016-3930-2. Epub 2017 Apr 6. PMID- 26269185 OWN - NLM STAT- MEDLINE DCOM- 20151215 LR - 20221207 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 20 DP - 2015 Oct TI - Severe Acute Respiratory Syndrome (SARS) Coronavirus ORF8 Protein Is Acquired from SARS-Related Coronavirus from Greater Horseshoe Bats through Recombination. PG - 10532-47 LID - 10.1128/JVI.01048-15 [doi] AB - Despite the identification of horseshoe bats as the reservoir of severe acute respiratory syndrome (SARS)-related coronaviruses (SARSr-CoVs), the origin of SARS-CoV ORF8, which contains the 29-nucleotide signature deletion among human strains, remains obscure. Although two SARS-related Rhinolophus sinicus bat CoVs (SARSr-Rs-BatCoVs) previously detected in Chinese horseshoe bats (Rhinolophus sinicus) in Yunnan, RsSHC014 and Rs3367, possessed 95% genome identities to human and civet SARSr-CoVs, their ORF8 protein exhibited only 32.2 to 33% amino acid identities to that of human/civet SARSr-CoVs. To elucidate the origin of SARS-CoV ORF8, we sampled 348 bats of various species in Yunnan, among which diverse alphacoronaviruses and betacoronaviruses, including potentially novel CoVs, were identified, with some showing potential interspecies transmission. The genomes of two betacoronaviruses, SARSr-Rf-BatCoV YNLF_31C and YNLF_34C, from greater horseshoe bats (Rhinolophus ferrumequinum), possessed 93% nucleotide identities to human/civet SARSr-CoV genomes. Although these two betacoronaviruses displayed lower similarities than SARSr-Rs-BatCoV RsSHC014 and Rs3367 in S protein to civet SARSr-CoVs, their ORF8 proteins demonstrated exceptionally high (80.4 to 81.3%) amino acid identities to that of human/civet SARSr-CoVs, compared to SARSr-BatCoVs from other horseshoe bats (23.2 to 37.3%). Potential recombination events were identified around ORF8 between SARSr-Rf-BatCoVs and SARSr-Rs-BatCoVs, leading to the generation of civet SARSr-CoVs. The expression of ORF8 subgenomic mRNA suggested that the ORF8 protein may be functional in SARSr-Rf-BatCoVs. The high Ka/Ks ratio among human SARS-CoVs compared to that among SARSr-BatCoVs supported that ORF8 is under strong positive selection during animal-to-human transmission. Molecular clock analysis using ORF1ab showed that SARSr-Rf-BatCoV YNLF_31C and YNLF_34C diverged from civet/human SARSr-CoVs in approximately 1990. SARS-CoV ORF8 originated from SARSr-CoVs of greater horseshoe bats through recombination, which may be important for animal-to-human transmission. IMPORTANCE: Although horseshoe bats are the primary reservoir of SARS-related coronaviruses (SARSr-CoVs), it is still unclear how these bat viruses have evolved to cross the species barrier to infect civets and humans. Most human SARS-CoV epidemic strains contain a signature 29-nucleotide deletion in ORF8, compared to civet SARSr-CoVs, suggesting that ORF8 may be important for interspecies transmission. However, the origin of SARS-CoV ORF8 remains obscure. In particular, SARSr-Rs-BatCoVs from Chinese horseshoe bats (Rhinolophus sinicus) exhibited <40% amino acid identities to human/civet SARS-CoV in the ORF8 protein. We detected diverse alphacoronaviruses and betacoronaviruses among various bat species in Yunnan, China, including two SARSr-Rf-BatCoVs from greater horseshoe bats that possessed ORF8 proteins with exceptionally high amino acid identities to that of human/civet SARSr-CoVs. We demonstrated recombination events around ORF8 between SARSr-Rf-BatCoVs and SARSr-Rs-BatCoVs, leading to the generation of civet SARSr-CoVs. Our findings offer insight into the evolutionary origin of SARS-CoV ORF8 protein, which was likely acquired from SARSr-CoVs of greater horseshoe bats through recombination. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Lau, Susanna K P AU - Lau SK AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Feng, Yun AU - Feng Y AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan, China Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, Yunnan, China. FAU - Chen, Honglin AU - Chen H AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Luk, Hayes K H AU - Luk HK AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Yang, Wei-Hong AU - Yang WH AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan, China Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, Yunnan, China. FAU - Li, Kenneth S M AU - Li KS AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Zhang, Yu-Zhen AU - Zhang YZ AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan, China Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, Yunnan, China. FAU - Huang, Yi AU - Huang Y AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Song, Zhi-Zhong AU - Song ZZ AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan, China Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, Yunnan, China. FAU - Chow, Wang-Ngai AU - Chow WN AUID- ORCID: 0000-0003-1275-2464 AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Fan, Rachel Y Y AU - Fan RY AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Ahmed, Syed Shakeel AU - Ahmed SS AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Yeung, Hazel C AU - Yeung HC AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Lam, Carol S F AU - Lam CS AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Cai, Jian-Piao AU - Cai JP AD - Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Wong, Samson S Y AU - Wong SS AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Chan, Jasper F W AU - Chan JF AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Yuen, Kwok-Yung AU - Yuen KY AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China. FAU - Zhang, Hai-Lin AU - Zhang HL AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan, China Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali, Yunnan, China pcywoo@hku.hk zhangHL715@163.com. FAU - Woo, Patrick C Y AU - Woo PC AD - State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China Department of Microbiology, The University of Hong Kong, Hong Kong, China pcywoo@hku.hk zhangHL715@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150812 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (RNA, Messenger) RN - 0 (RNA, Viral) RN - 0 (Viral Matrix Proteins) RN - 0 (sars7a protein, SARS virus) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - China MH - Chiroptera/virology MH - Coronavirus Infections/genetics/transmission/*veterinary/virology MH - Evolution, Molecular MH - Gene Expression MH - *Genome, Viral MH - Humans MH - Molecular Sequence Data MH - Phylogeny MH - Phylogeography MH - RNA, Messenger/genetics/metabolism MH - RNA, Viral/*genetics/metabolism MH - *Recombination, Genetic MH - Severe acute respiratory syndrome-related coronavirus/classification/*genetics/metabolism MH - Sequence Alignment MH - Sequence Homology, Nucleic Acid MH - Severe Acute Respiratory Syndrome/genetics/metabolism/transmission/virology MH - Viral Matrix Proteins/*genetics/metabolism MH - Viverridae/virology PMC - PMC4580176 EDAT- 2015/08/14 06:00 MHDA- 2015/12/17 06:00 PMCR- 2016/04/15 CRDT- 2015/08/14 06:00 PHST- 2015/05/09 00:00 [received] PHST- 2015/08/01 00:00 [accepted] PHST- 2015/08/14 06:00 [entrez] PHST- 2015/08/14 06:00 [pubmed] PHST- 2015/12/17 06:00 [medline] PHST- 2016/04/15 00:00 [pmc-release] AID - JVI.01048-15 [pii] AID - 01048-15 [pii] AID - 10.1128/JVI.01048-15 [doi] PST - ppublish SO - J Virol. 2015 Oct;89(20):10532-47. doi: 10.1128/JVI.01048-15. Epub 2015 Aug 12. PMID- 30781790 OWN - NLM STAT- MEDLINE DCOM- 20190820 LR - 20200309 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 11 IP - 2 DP - 2019 Feb 13 TI - Interferon Regulatory Factor 3-Mediated Signaling Limits Middle-East Respiratory Syndrome (MERS) Coronavirus Propagation in Cells from an Insectivorous Bat. LID - 10.3390/v11020152 [doi] LID - 152 AB - Insectivorous bats are speculated to be ancestral hosts of Middle-East respiratory syndrome (MERS) coronavirus (CoV). MERS-CoV causes disease in humans with thirty-five percent fatality, and has evolved proteins that counteract human antiviral responses. Since bats experimentally infected with MERS-CoV do not develop signs of disease, we tested the hypothesis that MERS-CoV would replicate less efficiently in bat cells than in human cells because of its inability to subvert antiviral responses in bat cells. We infected human and bat (Eptesicus fuscus) cells with MERS-CoV and observed that the virus grew to higher titers in human cells. MERS-CoV also effectively suppressed the antiviral interferon beta (IFNβ) response in human cells, unlike in bat cells. To determine if IRF3, a critical mediator of the interferon response, also regulated the response in bats, we examined the response of IRF3 to poly(I:C), a synthetic analogue of viral double-stranded RNA. We observed that bat IRF3 responded to poly(I:C) by nuclear translocation and post-translational modifications, hallmarks of IRF3 activation. Suppression of IRF3 by small-interfering RNA (siRNA) demonstrated that IRF3 was critical for poly(I:C) and MERS-CoV induced induction of IFNβ in bat cells. Our study demonstrates that innate antiviral signaling in E. fuscus bat cells is resistant to MERS-CoV-mediated subversion. FAU - Banerjee, Arinjay AU - Banerjee A AUID- ORCID: 0000-0002-2821-8357 AD - Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada. banera9@mcmaster.ca. FAU - Falzarano, Darryl AU - Falzarano D AD - Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada. darryl.falzarano@usask.ca. AD - Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-Intervac), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada. darryl.falzarano@usask.ca. FAU - Rapin, Noreen AU - Rapin N AD - Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada. noreen.rapin@usask.ca. FAU - Lew, Jocelyne AU - Lew J AD - Vaccine and Infectious Disease Organization-International Vaccine Centre (VIDO-Intervac), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada. jocelyne.lew@usask.ca. FAU - Misra, Vikram AU - Misra V AUID- ORCID: 0000-0001-6818-7156 AD - Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada. vikram.misra@usask.ca. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190213 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (IRF3 protein, human) RN - 0 (Interferon Regulatory Factor-3) RN - 77238-31-4 (Interferon-beta) MH - Animals MH - Cell Line MH - Chiroptera/*virology MH - Gene Knockdown Techniques MH - Gene Knockout Techniques MH - Humans MH - Immune Evasion MH - *Immunity, Innate MH - Interferon Regulatory Factor-3/genetics/*immunology MH - Interferon-beta/immunology MH - Kidney/cytology/virology MH - Middle East Respiratory Syndrome Coronavirus/*immunology/pathogenicity MH - Phylogeny MH - Signal Transduction PMC - PMC6410008 OTO - NOTNLM OT - IRF3 OT - MERS-CoV OT - bat OT - interferon COIS- The authors declare no competing interest. EDAT- 2019/02/20 06:00 MHDA- 2019/08/21 06:00 PMCR- 2019/02/01 CRDT- 2019/02/21 06:00 PHST- 2018/12/10 00:00 [received] PHST- 2019/02/08 00:00 [revised] PHST- 2019/02/11 00:00 [accepted] PHST- 2019/02/21 06:00 [entrez] PHST- 2019/02/20 06:00 [pubmed] PHST- 2019/08/21 06:00 [medline] PHST- 2019/02/01 00:00 [pmc-release] AID - v11020152 [pii] AID - viruses-11-00152 [pii] AID - 10.3390/v11020152 [doi] PST - epublish SO - Viruses. 2019 Feb 13;11(2):152. doi: 10.3390/v11020152. PMID- 26954467 OWN - NLM STAT- MEDLINE DCOM- 20161012 LR - 20221207 IS - 1873-2518 (Electronic) IS - 0264-410X (Print) IS - 0264-410X (Linking) VI - 34 IP - 17 DP - 2016 Apr 12 TI - Memory T cell responses targeting the SARS coronavirus persist up to 11 years post-infection. PG - 2008-14 LID - S0264-410X(16)00258-9 [pii] LID - 10.1016/j.vaccine.2016.02.063 [doi] AB - Severe acute respiratory syndrome (SARS) is a highly contagious infectious disease which first emerged in late 2002, caused by a then novel human coronavirus, SARS coronavirus (SARS-CoV). The virus is believed to have originated from bats and transmitted to human through intermediate animals such as civet cats. The re-emergence of SARS-CoV remains a valid concern due to the continual persistence of zoonotic SARS-CoVs and SARS-like CoVs (SL-CoVs) in bat reservoirs. In this study, the screening for the presence of SARS-specific T cells in a cohort of three SARS-recovered individuals at 9 and 11 years post-infection was carried out, and all memory T cell responses detected target the SARS-CoV structural proteins. Two CD8(+) T cell responses targeting the SARS-CoV membrane (M) and nucleocapsid (N) proteins were characterized by determining their HLA restriction and minimal T cell epitope regions. Furthermore, these responses were found to persist up to 11 years post-infection. An absence of cross-reactivity of these CD8(+) T cell responses against the newly-emerged Middle East respiratory syndrome coronavirus (MERS-CoV) was also demonstrated. The knowledge of the persistence of SARS-specific celullar immunity targeting the viral structural proteins in SARS-recovered individuals is important in the design and development of SARS vaccines, which are currently unavailable. CI - Copyright © 2016 Elsevier Ltd. All rights reserved. FAU - Ng, Oi-Wing AU - Ng OW AD - Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore. FAU - Chia, Adeline AU - Chia A AD - Program Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore. FAU - Tan, Anthony T AU - Tan AT AD - Program Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore. FAU - Jadi, Ramesh S AU - Jadi RS AD - Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore. FAU - Leong, Hoe Nam AU - Leong HN AD - Singapore General Hospital, Singapore. FAU - Bertoletti, Antonio AU - Bertoletti A AD - Program Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore; Viral Hepatitis Laboratory, Singapore Institute for Clinical Sciences, Agency of Science Technology and Research (A*STAR), Singapore. FAU - Tan, Yee-Joo AU - Tan YJ AD - Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore; Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Singapore. Electronic address: Yee_Joo_TAN@NUHS.edu.sg. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160305 PL - Netherlands TA - Vaccine JT - Vaccine JID - 8406899 RN - 0 (Coronavirus M Proteins) RN - 0 (Coronavirus Nucleocapsid Proteins) RN - 0 (Epitopes, T-Lymphocyte) RN - 0 (Histocompatibility Antigens Class I) RN - 0 (M protein, SARS-CoV) RN - 0 (Nucleocapsid Proteins) RN - 0 (Viral Matrix Proteins) SB - IM MH - CD8-Positive T-Lymphocytes/*immunology MH - Coronavirus M Proteins MH - Coronavirus Nucleocapsid Proteins MH - Cross Reactions MH - Epitopes, T-Lymphocyte/immunology MH - Histocompatibility Antigens Class I/immunology MH - Humans MH - *Immunity, Cellular MH - *Immunologic Memory MH - Middle East Respiratory Syndrome Coronavirus MH - Nucleocapsid Proteins/immunology MH - Severe acute respiratory syndrome-related coronavirus MH - Severe Acute Respiratory Syndrome/*immunology/prevention & control MH - Viral Matrix Proteins/immunology PMC - PMC7115611 OTO - NOTNLM OT - Epitope OT - Immunity OT - SARS-CoV OT - T cell EDAT- 2016/03/10 06:00 MHDA- 2016/10/13 06:00 PMCR- 2016/03/05 CRDT- 2016/03/09 06:00 PHST- 2015/09/26 00:00 [received] PHST- 2016/02/12 00:00 [revised] PHST- 2016/02/24 00:00 [accepted] PHST- 2016/03/09 06:00 [entrez] PHST- 2016/03/10 06:00 [pubmed] PHST- 2016/10/13 06:00 [medline] PHST- 2016/03/05 00:00 [pmc-release] AID - S0264-410X(16)00258-9 [pii] AID - 10.1016/j.vaccine.2016.02.063 [doi] PST - ppublish SO - Vaccine. 2016 Apr 12;34(17):2008-14. doi: 10.1016/j.vaccine.2016.02.063. Epub 2016 Mar 5. PMID- 27279618 OWN - NLM STAT- MEDLINE DCOM- 20170508 LR - 20181113 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 90 IP - 16 DP - 2016 Aug 15 TI - Infectious Bronchitis Coronavirus Limits Interferon Production by Inducing a Host Shutoff That Requires Accessory Protein 5b. PG - 7519-7528 LID - 10.1128/JVI.00627-16 [doi] AB - During infection of their host cells, viruses often inhibit the production of host proteins, a process that is referred to as host shutoff. By doing this, viruses limit the production of antiviral proteins and increase production capacity for viral proteins. Coronaviruses from the genera Alphacoronavirus and Betacoronavirus, such as severe acute respiratory syndrome coronavirus (SARS-CoV), establish host shutoff via their nonstructural protein 1 (nsp1). The Gammacoronavirus and Deltacoronavirus genomes, however, do not encode nsp1, and it has been suggested that these viruses do not induce host shutoff. Here, we show that the Gammacoronavirus infectious bronchitis virus (IBV) does induce host shutoff, and we find that its accessory protein 5b is indispensable for this function. Importantly, we found that 5b-null viruses, unlike wild-type viruses, induce production of high concentrations of type I interferon protein in vitro, indicating that host shutoff by IBV plays an important role in antagonizing the host's innate immune response. Altogether, we demonstrate that 5b is a functional equivalent of nsp1, thereby answering the longstanding question of whether lack of nsp1 in gammacoronaviruses is compensated for by another viral protein. As such, our study is a significant step forward in the understanding of coronavirus biology and closes a gap in the understanding of some IBV virulence strategies. IMPORTANCE: Many viruses inhibit protein synthesis by their host cell to enhance virus replication and to antagonize antiviral defense mechanisms. This process is referred to as host shutoff. We studied gene expression and protein synthesis in chicken cells infected with the important poultry pathogen infectious bronchitis virus (IBV). We show that IBV inhibits synthesis of host proteins, including that of type I interferon, a key component of the antiviral response. The IBV-induced host shutoff, however, does not require degradation of host RNA. Furthermore, we demonstrate that accessory protein 5b of IBV plays a crucial role in the onset of host shutoff. Our findings suggest that inhibition of host protein synthesis is a common feature of coronaviruses and primarily serves to inhibit the antiviral response of the host. CI - Copyright © 2016, American Society for Microbiology. All Rights Reserved. FAU - Kint, Joeri AU - Kint J AD - Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands. AD - MSD Animal Health, Bioprocess Technology and Support, Boxmeer, The Netherlands. FAU - Langereis, Martijn A AU - Langereis MA AD - Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. FAU - Maier, Helena J AU - Maier HJ AD - Avian Viral Diseases, The Pirbright Institute, Compton Laboratory, Pirbright, United Kingdom. FAU - Britton, Paul AU - Britton P AD - Avian Viral Diseases, The Pirbright Institute, Compton Laboratory, Pirbright, United Kingdom. FAU - van Kuppeveld, Frank J AU - van Kuppeveld FJ AD - Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. FAU - Koumans, Joseph AU - Koumans J AD - MSD Animal Health, Bioprocess Technology and Support, Boxmeer, The Netherlands. FAU - Wiegertjes, Geert F AU - Wiegertjes GF AD - Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands. FAU - Forlenza, Maria AU - Forlenza M AUID- ORCID: 0000-0001-9026-7320 AD - Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands maria.forlenza@wur.nl. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160727 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Interferon Type I) RN - 0 (Viral Proteins) SB - IM MH - Animals MH - Cells, Cultured MH - Chickens MH - Gene Knockout Techniques MH - *Host-Pathogen Interactions MH - *Immune Evasion MH - Infectious bronchitis virus/genetics/*immunology/*pathogenicity MH - Interferon Type I/*antagonists & inhibitors MH - Viral Proteins/genetics/*metabolism PMC - PMC4984617 EDAT- 2016/06/10 06:00 MHDA- 2017/05/10 06:00 PMCR- 2017/01/27 CRDT- 2016/06/10 06:00 PHST- 2016/04/03 00:00 [received] PHST- 2016/06/01 00:00 [accepted] PHST- 2016/06/10 06:00 [entrez] PHST- 2016/06/10 06:00 [pubmed] PHST- 2017/05/10 06:00 [medline] PHST- 2017/01/27 00:00 [pmc-release] AID - JVI.00627-16 [pii] AID - 00627-16 [pii] AID - 10.1128/JVI.00627-16 [doi] PST - epublish SO - J Virol. 2016 Jul 27;90(16):7519-7528. doi: 10.1128/JVI.00627-16. Print 2016 Aug 15. PMID- 28883450 OWN - NLM STAT- MEDLINE DCOM- 20190528 LR - 20190528 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 7 IP - 1 DP - 2017 Sep 7 TI - Virome analysis for identification of novel mammalian viruses in bats from Southeast China. PG - 10917 LID - 10.1038/s41598-017-11384-w [doi] LID - 10917 AB - Bats have been shown as important mammal resevoirs to carry a variety of zoonotic pathogens. To analyze pathogenic species in bats from southeast coastal regions of China, we performed metagenomic sequencing technology for high throughput sequencing of six sentinels from southeast coastal area of China. We obtained 5,990,261 high quality reads from intestine and lung tissue of 235 bats, including 2,975,371 assembled sequences. 631,490 reads predicted overlapping sequences for the open reading frame (ORF), which accounts for 2.37% of all the sequences (15,012/631,490). Further, the acquired virus sequences were classified into 25 viral families, including 16 vertebrate viruses, four plant viruses and five insect viruses. All bat samples were screened by specific PCR and phylogenetic analysis. Using these techniques, we discovered many novel bat viruses and some bat viruses closely-related to known human/animal pathogens, including coronavirus, norovirus, adenovirus, bocavirus, astrovirus, and circovirus. In summary, this study extended our understanding of bats as the viral reservoirs. Additionally, it also provides a basis for furher studying the transmission of viruses from bats to humans. FAU - Hu, Dan AU - Hu D AD - Department of Epidemiology, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China. AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Zhu, Changqiang AU - Zhu C AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Wang, Yi AU - Wang Y AD - Department of Epidemiology, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China. AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Ai, Lele AU - Ai L AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Yang, Lu AU - Yang L AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Ye, Fuqiang AU - Ye F AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Ding, Chenxi AU - Ding C AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Chen, Jiafeng AU - Chen J AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - He, Biao AU - He B AD - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin, China. FAU - Zhu, Jin AU - Zhu J AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. FAU - Qian, Hui AU - Qian H AD - Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China. FAU - Xu, Wenrong AU - Xu W AD - Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China. FAU - Feng, Youjun AU - Feng Y AD - College of Animal Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China. FAU - Tan, Weilong AU - Tan W AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. njcdc@163.com. FAU - Wang, Changjun AU - Wang C AD - Department of Epidemiology, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China. science2008@hotmail.com. AD - Department of Epidemiology, Research Institute for Medicine of Nanjing Command, Nanjing, 210002, China. science2008@hotmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20170907 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 SB - IM MH - Animals MH - China MH - Chiroptera/*virology MH - Intestines/virology MH - Lung/virology MH - Metagenomics MH - Phylogeny MH - Polymerase Chain Reaction MH - Sequence Analysis, DNA MH - Virus Diseases/*veterinary/virology MH - Viruses/*classification/genetics/*isolation & purification MH - Zoonoses/*virology PMC - PMC5589946 COIS- The authors declare that they have no competing interests. EDAT- 2017/09/09 06:00 MHDA- 2019/05/29 06:00 PMCR- 2017/09/07 CRDT- 2017/09/09 06:00 PHST- 2017/04/13 00:00 [received] PHST- 2017/08/23 00:00 [accepted] PHST- 2017/09/09 06:00 [entrez] PHST- 2017/09/09 06:00 [pubmed] PHST- 2019/05/29 06:00 [medline] PHST- 2017/09/07 00:00 [pmc-release] AID - 10.1038/s41598-017-11384-w [pii] AID - 11384 [pii] AID - 10.1038/s41598-017-11384-w [doi] PST - epublish SO - Sci Rep. 2017 Sep 7;7(1):10917. doi: 10.1038/s41598-017-11384-w. PMID- 28220326 OWN - NLM STAT- MEDLINE DCOM- 20170720 LR - 20221207 IS - 1432-8798 (Electronic) IS - 0304-8608 (Print) IS - 0304-8608 (Linking) VI - 162 IP - 6 DP - 2017 Jun TI - Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR. PG - 1617-1623 LID - 10.1007/s00705-017-3281-9 [doi] AB - Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50-100 copies/mL and 5-100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats. FAU - Noh, Ji Yeong AU - Noh JY AD - Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. AD - College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea. FAU - Yoon, Sun-Woo AU - Yoon SW AD - Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. AD - Bio-Analytical Science Division, Korea University of Science and Technology (UST), Daejeon, Republic of Korea. FAU - Kim, Doo-Jin AU - Kim DJ AD - Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. FAU - Lee, Moo-Seung AU - Lee MS AD - Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. AD - Bio-Analytical Science Division, Korea University of Science and Technology (UST), Daejeon, Republic of Korea. FAU - Kim, Ji-Hyung AU - Kim JH AD - Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. FAU - Na, Woonsung AU - Na W AD - Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea. FAU - Song, Daesub AU - Song D AD - Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea. FAU - Jeong, Dae Gwin AU - Jeong DG AD - Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. dgjeong@kribb.re.kr. AD - Bio-Analytical Science Division, Korea University of Science and Technology (UST), Daejeon, Republic of Korea. dgjeong@kribb.re.kr. FAU - Kim, Hye Kwon AU - Kim HK AD - Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. khk1329@kribb.re.kr. LA - eng PT - Journal Article DEP - 20170220 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 RN - 0 (RNA, Viral) SB - IM EIN - Arch Virol. 2018 Mar;163(3):819. doi: 10.1007/s00705-017-3677-6. PMID: 29273879 MH - Animals MH - Chiroptera/virology MH - Coronaviridae/genetics/*isolation & purification MH - Coronavirus Infections/virology MH - Diagnosis, Differential MH - Feces/virology MH - Humans MH - Limit of Detection MH - Middle East Respiratory Syndrome Coronavirus/genetics/*isolation & purification MH - RNA, Viral/genetics MH - Real-Time Polymerase Chain Reaction/*methods MH - Reverse Transcriptase Polymerase Chain Reaction/*methods MH - Severe acute respiratory syndrome-related coronavirus/genetics/*isolation & purification MH - Sequence Alignment MH - Severe Acute Respiratory Syndrome/virology MH - Sputum/virology PMC - PMC7086956 COIS- The authors have declared that no competing interests exist. EDAT- 2017/02/22 06:00 MHDA- 2017/07/21 06:00 PMCR- 2020/03/23 CRDT- 2017/02/22 06:00 PHST- 2016/11/17 00:00 [received] PHST- 2017/02/02 00:00 [accepted] PHST- 2017/02/22 06:00 [pubmed] PHST- 2017/07/21 06:00 [medline] PHST- 2017/02/22 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s00705-017-3281-9 [pii] AID - 3281 [pii] AID - 10.1007/s00705-017-3281-9 [doi] PST - ppublish SO - Arch Virol. 2017 Jun;162(6):1617-1623. doi: 10.1007/s00705-017-3281-9. Epub 2017 Feb 20. PMID- 31113572 OWN - NLM STAT- MEDLINE DCOM- 20190726 LR - 20200407 IS - 1532-2971 (Electronic) IS - 1090-0233 (Print) IS - 1090-0233 (Linking) VI - 248 DP - 2019 Jun TI - Clinical presentation, diagnostic findings, and outcome of adult horses with equine coronavirus infection at a veterinary teaching hospital: 33 cases (2012-2018). PG - 95-100 LID - S1090-0233(18)30328-9 [pii] LID - 10.1016/j.tvjl.2019.05.001 [doi] AB - Equine coronavirus (ECoV) is a recently described enteric virus with worldwide outbreaks; however, there are little data available on clinical presentation, diagnosis, and outcome. The study objective was to document case management of ECoV in adult horses presented to a referral hospital and compare to a cohort of horses that tested negative for ECoV. A retrospective case series was performed based on positive real-time quantitative PCR results for ECoV on faeces from horses treated at the UC Davis Veterinary Medical Teaching Hospital from 1 March 2012 to 31 March 2018. Horses negative for ECoV were matched to the ECoV-positive group as controls. Data collected included signalment, history, exam findings, diagnostics, treatment, and follow-up. Thirty-three horses (median age, 11 years; range, 2-37 years) tested ECoV-positive, including three horses with co-infections. Presenting complaints for ECoV-infected horses included historic fevers (n = 25/30; 83%), anorexia (n = 14/30; 47%), and colic (n = 13/30; 43%). ECoV-positive horses had significantly lower white blood cell (median, 3.0 × 10(9)/L; range, 0.68-16.2 × 10(9)/L), neutrophil (median, 1.26 × 10(9)/L; range, 0.15-14.4 × 10(9)/L), and lymphocyte (median, 0.86 × 10(9)/L; range, 0.42-3.47 × 10(9)/L) counts than ECoV-negative horses. Electrolyte and metabolic derangements and scant faeces were common. Twenty-seven horses were hospitalised for a median of 5 days (range, 0.5-14 days), with 26/27 (96%) horses surviving to discharge. ECoV infection should be a differential diagnosis for adult horses with fever, colic, anorexia, and leukopenia. The disease has a low mortality rate, but horses may require intensive care to resolve severe leukopenia, systemic inflammation, and metabolic disturbances. CI - Copyright © 2019 Elsevier Ltd. All rights reserved. FAU - Berryhill, E H AU - Berryhill EH AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, One Shields Ave., Davis, CA, 95616, USA. Electronic address: ehberryhill@vmth.ucdavis.edu. FAU - Magdesian, K G AU - Magdesian KG AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, One Shields Ave., Davis, CA, 95616, USA. FAU - Aleman, M AU - Aleman M AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, One Shields Ave., Davis, CA, 95616, USA. FAU - Pusterla, N AU - Pusterla N AD - Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, One Shields Ave., Davis, CA, 95616, USA. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20190504 PL - England TA - Vet J JT - Veterinary journal (London, England : 1997) JID - 9706281 EIN - Vet J. 2019 Aug;250:14. doi: 10.1016/j.tvjl.2019.05.014. PMID: 31383414 MH - Animals MH - Betacoronavirus 1/*isolation & purification MH - California/epidemiology MH - Coronavirus Infections/epidemiology/*veterinary MH - Disease Outbreaks/*veterinary MH - Female MH - Horse Diseases/*epidemiology/etiology MH - Horses MH - Hospitals, Animal MH - Male MH - Records/veterinary PMC - PMC7110482 OTO - NOTNLM OT - Colic OT - Diarrhea OT - Fever OT - Gastrointestinal OT - Infectious EDAT- 2019/05/23 06:00 MHDA- 2019/07/28 06:00 PMCR- 2019/05/04 CRDT- 2019/05/23 06:00 PHST- 2018/06/27 00:00 [received] PHST- 2019/04/24 00:00 [revised] PHST- 2019/05/03 00:00 [accepted] PHST- 2019/05/23 06:00 [entrez] PHST- 2019/05/23 06:00 [pubmed] PHST- 2019/07/28 06:00 [medline] PHST- 2019/05/04 00:00 [pmc-release] AID - S1090-0233(18)30328-9 [pii] AID - 10.1016/j.tvjl.2019.05.001 [doi] PST - ppublish SO - Vet J. 2019 Jun;248:95-100. doi: 10.1016/j.tvjl.2019.05.001. Epub 2019 May 4. PMID- 25288733 OWN - NLM STAT- MEDLINE DCOM- 20150424 LR - 20250104 IS - 1091-6490 (Electronic) IS - 0027-8424 (Print) IS - 0027-8424 (Linking) VI - 111 IP - 42 DP - 2014 Oct 21 TI - Host cell entry of Middle East respiratory syndrome coronavirus after two-step, furin-mediated activation of the spike protein. PG - 15214-9 LID - 10.1073/pnas.1407087111 [doi] AB - Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly identified betacoronavirus causing high morbidity and mortality in humans. The coronavirus spike (S) protein is the main determinant of viral entry, and although it was previously shown that MERS-CoV S can be activated by various proteases, the details of the mechanisms of proteolytic activation of fusion are still incompletely characterized. Here, we have uncovered distinctive characteristics of MERS-CoV S. We identify, by bioinformatics and peptide cleavage assays, two cleavage sites for furin, a ubiquitously expressed protease, which are located at the S1/S2 interface and at the S2' position of the S protein. We show that although the S1/S2 site is proteolytically processed by furin during protein biosynthesis, the S2' site is cleaved upon viral entry. MERS-CoV pseudovirion infection was shown to be enhanced by elevated levels of furin expression, and entry could be decreased by furin siRNA silencing. Enhanced furin activity appeared to partially override the low pH-dependent nature of MERS-CoV entry. Inhibition of furin activity was shown to decrease MERS-CoV S-mediated entry, as well as infection by the virus. Overall, we show that MERS-CoV has evolved an unusual two-step furin activation for fusion, suggestive of a role during the process of emergence into the human population. The ability of MERS-CoV to use furin in this manner, along with other proteases, may explain the polytropic nature of the virus. FAU - Millet, Jean Kaoru AU - Millet JK AD - Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853. FAU - Whittaker, Gary R AU - Whittaker GR AD - Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853 grw7@cornell.edu. LA - eng GR - R21 AI111085/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20141006 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (RNA, Small Interfering) RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.- (Peptide Hydrolases) RN - EC 3.4.21.75 (Furin) SB - IM MH - Animals MH - Cell Line, Tumor MH - Chlorocebus aethiops MH - Computational Biology MH - Furin/chemistry MH - Gene Silencing MH - Genetic Predisposition to Disease MH - HEK293 Cells MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*physiology MH - Mutation MH - Peptide Hydrolases/metabolism MH - RNA, Small Interfering/metabolism MH - Receptors, Virus/metabolism MH - Spike Glycoprotein, Coronavirus/*metabolism MH - Time Factors MH - Vero Cells MH - *Virus Internalization PMC - PMC4210292 OTO - NOTNLM OT - Middle East respiratory syndrome coronavirus OT - furin OT - proteolytic activation OT - spike protein OT - virus entry COIS- The authors declare no conflict of interest. EDAT- 2014/10/08 06:00 MHDA- 2015/04/25 06:00 PMCR- 2015/04/21 CRDT- 2014/10/08 06:00 PHST- 2014/10/08 06:00 [entrez] PHST- 2014/10/08 06:00 [pubmed] PHST- 2015/04/25 06:00 [medline] PHST- 2015/04/21 00:00 [pmc-release] AID - 1407087111 [pii] AID - 201407087 [pii] AID - 10.1073/pnas.1407087111 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):15214-9. doi: 10.1073/pnas.1407087111. Epub 2014 Oct 6. PMID- 25991685 OWN - NLM STAT- MEDLINE DCOM- 20160111 LR - 20240324 IS - 2150-7511 (Electronic) VI - 6 IP - 3 DP - 2015 May 19 TI - A novel pathogenic Mammalian orthoreovirus from diarrheic pigs and Swine blood meal in the United States. PG - e00593-15 LID - 10.1128/mBio.00593-15 [doi] LID - e00593-15 AB - Since May 2013, outbreaks of porcine epidemic diarrhea have devastated the U.S. swine industry, causing immense economic losses. Two different swine enteric coronaviruses (porcine epidemic diarrhea virus and Delta coronavirus) have been isolated from the affected swine population. The disease has been reported from at least 32 states of the United States and other countries, including Mexico, Peru, Dominican Republic, Canada, Columbia, Ecuador, and Ukraine, with repeated outbreaks in previously infected herds. Here we report the isolation and characterization of a novel mammalian orthoreovirus 3 (MRV3) from diarrheic feces of piglets from these outbreaks in three states and ring-dried swine blood meal from multiple sources. MRV3 could not be isolated from healthy or pigs that had recovered from epidemic diarrhea from four states. Several MRV3 isolates were obtained from chloroform-extracted pig feces or blood meal in cell cultures or developing chicken embryos. Biological characterization of two representative isolates revealed trypsin resistance and thermostability at 90°C. NextGen sequencing of ultrapurified viruses indicated a strong homology of the S1 segment to mammalian and bat MRV3. Neonatal piglets experimentally infected with these viruses or a chloroform extract of swine blood meal developed severe diarrhea and acute gastroenteritis with 100% mortality within 3 days postinfection. Therefore, the novel porcine MRV3 may contribute to enteric disease along with other swine enteric viruses. The role of MRV3 in the current outbreaks of porcine epidemic diarrhea in the United States remains to be determined, but the pathogenic nature of the virus warrants further investigations on its epidemiology and prevalence. IMPORTANCE: Porcine orthoreoviruses causing diarrhea have been reported in China and Korea but not in the United States. We have isolated and characterized two pathogenic reassortant MRV3 isolates from swine fecal samples from porcine epidemic diarrhea outbreaks and ring-dried swine blood meal in the United States. These fecal and blood meal isolates or a chloroform extract of blood meal induced severe diarrhea and mortality in experimentally infected neonatal pigs. Genetic and phylogenetic analyses of two MRV3 isolates revealed that they are identical but differed significantly from nonpathogenic mammalian orthoreoviruses circulating in the United States. The present study provides a platform for immediate development of suitable vaccines and diagnostics to prevent and control porcine orthoreovirus diarrhea. CI - Copyright © 2015 Thimmasandra Narayanappa et al. FAU - Thimmasandra Narayanappa, Athmaram AU - Thimmasandra Narayanappa A AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Sooryanarain, Harini AU - Sooryanarain H AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Deventhiran, Jagadeeswaran AU - Deventhiran J AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Cao, Dianjun AU - Cao D AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Ammayappan Venkatachalam, Backiyalakshmi AU - Ammayappan Venkatachalam B AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Kambiranda, Devaiah AU - Kambiranda D AD - Center for Viticulture and Small Fruits, Florida A&M University, Tallahassee, Florida, USA. FAU - LeRoith, Tanya AU - LeRoith T AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Heffron, Connie Lynn AU - Heffron CL AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Lindstrom, Nicole AU - Lindstrom N AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Hall, Karen AU - Hall K AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Jobst, Peter AU - Jobst P AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Sexton, Cary AU - Sexton C AD - Livestock Veterinary Services, Kinston, North Carolina, USA. FAU - Meng, Xiang-Jin AU - Meng XJ AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. FAU - Elankumaran, Subbiah AU - Elankumaran S AD - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA kumarans@vt.edu. LA - eng SI - GENBANK/KM820744 SI - GENBANK/KM820745 SI - GENBANK/KM820746 SI - GENBANK/KM820747 SI - GENBANK/KM820748 SI - GENBANK/KM820749 SI - GENBANK/KM820750 SI - GENBANK/KM820751 SI - GENBANK/KM820752 SI - GENBANK/KM820753 SI - GENBANK/KM820754 SI - GENBANK/KM820755 SI - GENBANK/KM820756 SI - GENBANK/KM820757 SI - GENBANK/KM820758 SI - GENBANK/KM820759 SI - GENBANK/KM820760 SI - GENBANK/KM820761 SI - GENBANK/KM820762 SI - GENBANK/KM820763 PT - Journal Article DEP - 20150519 PL - United States TA - mBio JT - mBio JID - 101519231 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Blood/*virology MH - Cluster Analysis MH - Diarrhea/*veterinary/virology MH - Feces/*virology MH - Mammalian orthoreovirus 3/*classification/genetics/*isolation & purification/physiology MH - Molecular Sequence Data MH - Phylogeny MH - RNA, Viral/genetics MH - Sequence Analysis, DNA MH - Sequence Homology MH - Swine MH - Swine Diseases/*virology MH - United States MH - Virus Cultivation PMC - PMC4442135 EDAT- 2015/05/21 06:00 MHDA- 2016/01/12 06:00 PMCR- 2015/05/19 CRDT- 2015/05/21 06:00 PHST- 2015/05/21 06:00 [entrez] PHST- 2015/05/21 06:00 [pubmed] PHST- 2016/01/12 06:00 [medline] PHST- 2015/05/19 00:00 [pmc-release] AID - mBio.00593-15 [pii] AID - mBio00593-15 [pii] AID - 10.1128/mBio.00593-15 [doi] PST - epublish SO - mBio. 2015 May 19;6(3):e00593-15. doi: 10.1128/mBio.00593-15. PMID- 26733065 OWN - NLM STAT- MEDLINE DCOM- 20161012 LR - 20231213 IS - 2150-7511 (Electronic) VI - 7 IP - 1 DP - 2016 Jan 5 TI - Characterization of a Pathogenic Full-Length cDNA Clone and Transmission Model for Porcine Epidemic Diarrhea Virus Strain PC22A. PG - e01451-15 LID - 10.1128/mBio.01451-15 [doi] LID - e01451-15 AB - Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic alphacoronavirus. In the United States, highly virulent PEDV strains cause between 80 and 100% mortality in suckling piglets and are rapidly transmitted between animals and farms. To study the genetic factors that regulate pathogenesis and transmission, we developed a molecular clone of PEDV strain PC22A. The infectious-clone-derived PEDV (icPEDV) replicated as efficiently as the parental virus in cell culture and in pigs, resulting in lethal disease in vivo. Importantly, recombinant PEDV was rapidly transmitted to uninoculated pigs via indirect contact, demonstrating virulence and efficient transmission while replicating phenotypes seen in the wild-type virus. Using reverse genetics, we removed open reading frame 3 (ORF3) and replaced this region with a red fluorescent protein (RFP) gene to generate icPEDV-ΔORF3-RFP. icPEDV-ΔORF3-RFP replicated efficiently in vitro and in vivo, was efficiently transmitted among pigs, and produced lethal disease outcomes. However, the diarrheic scores in icPEDV-ΔORF3-RFP-infected pigs were lower than those in wild-type-virus- or icPEDV-infected pigs, and the virus formed smaller plaques than those of PC22A. Together, these data describe the development of a robust reverse-genetics platform for identifying genetic factors that regulate pathogenic outcomes and transmission efficiency in vivo, providing key infrastructural developments for developing and evaluating the efficacy of live attenuated vaccines and therapeutics in a clinical setting. IMPORTANCE: Porcine epidemic diarrhea virus (PEDV) emerged in the United States in 2013 and has since killed 10% of U.S. farm pigs. Though the disease has been circulating internationally for decades, the lack of a rapid reverse-genetics platform for manipulating PEDV and identifying genetic factors that impact transmission and virulence has hindered the study of this important agricultural disease. Here, we present a DNA-based infectious-clone system that replicates the pathogenesis of circulating U.S. strain PC22A both in vitro and in piglets. This infectious clone can be used both to study the genetics, virulence, and transmission of PEDV coronavirus and to inform the creation of a live attenuated PEDV vaccine. CI - Copyright © 2016 Beall et al. FAU - Beall, Anne AU - Beall A AD - University of North Carolina Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Yount, Boyd AU - Yount B AD - University of North Carolina Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Lin, Chun-Ming AU - Lin CM AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, USA. FAU - Hou, Yixuan AU - Hou Y AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, USA. FAU - Wang, Qiuhong AU - Wang Q AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, USA. FAU - Saif, Linda AU - Saif L AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio, USA. FAU - Baric, Ralph AU - Baric R AD - University of North Carolina Chapel Hill, Chapel Hill, North Carolina, USA rbaric@email.unc.edu. LA - eng GR - U19 AI109761/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20160105 PL - United States TA - mBio JT - mBio JID - 101519231 RN - 0 (DNA, Complementary) RN - 0 (DNA, Viral) RN - 0 (Luminescent Proteins) SB - IM MH - Animals MH - Cloning, Molecular MH - Coronavirus Infections/pathology/transmission/*veterinary/virology MH - DNA, Complementary/genetics MH - DNA, Viral/genetics MH - Diarrhea/pathology/virology MH - Gene Deletion MH - Luminescent Proteins/genetics/metabolism MH - Porcine epidemic diarrhea virus/*genetics/*pathogenicity MH - Recombination, Genetic MH - Reverse Genetics/*methods MH - Staining and Labeling MH - Survival Analysis MH - Swine MH - Swine Diseases/pathology/*transmission/*virology MH - United States MH - Virulence MH - Red Fluorescent Protein PMC - PMC4724997 EDAT- 2016/01/07 06:00 MHDA- 2016/10/13 06:00 PMCR- 2016/01/05 CRDT- 2016/01/07 06:00 PHST- 2016/01/07 06:00 [entrez] PHST- 2016/01/07 06:00 [pubmed] PHST- 2016/10/13 06:00 [medline] PHST- 2016/01/05 00:00 [pmc-release] AID - mBio.01451-15 [pii] AID - mBio01451-15 [pii] AID - 10.1128/mBio.01451-15 [doi] PST - epublish SO - mBio. 2016 Jan 5;7(1):e01451-15. doi: 10.1128/mBio.01451-15. PMID- 27637887 OWN - NLM STAT- MEDLINE DCOM- 20180509 LR - 20200418 IS - 1865-1682 (Electronic) IS - 1865-1674 (Print) IS - 1865-1674 (Linking) VI - 64 IP - 6 DP - 2017 Dec TI - Identification of a Lineage D Betacoronavirus in Cave Nectar Bats (Eonycteris spelaea) in Singapore and an Overview of Lineage D Reservoir Ecology in SE Asian Bats. PG - 1790-1800 LID - 10.1111/tbed.12568 [doi] AB - Coronaviruses are a diverse group of viruses that infect mammals and birds. Bats are reservoirs for several different coronaviruses in the Alphacoronavirus and Betacoronavirus genera. They also appear to be the natural reservoir for the ancestral viruses that generated the severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus outbreaks. Here, we detected coronavirus sequences in next-generation sequence data created from Eonycteris spelaea faeces and urine. We also screened by PCR urine samples, faecal samples and rectal swabs collected from six species of bats in Singapore between 2011 and 2014, all of which were negative. The phylogenetic analysis indicates this novel strain is most closely related to lineage D Betacoronaviruses detected in a diverse range of bat species. This is the second time that coronaviruses have been detected in cave nectar bats, but the first coronavirus sequence data generated from this species. Bat species from which this group of coronaviruses has been detected are widely distributed across SE Asia, South Asia and Southern China. They overlap geographically, often share roosting sites and have been witnessed to forage on the same plant. The addition of sequence data from this group of viruses will allow us to better understand coronavirus evolution and host specificity. CI - © 2016 Blackwell Verlag GmbH. FAU - Mendenhall, I H AU - Mendenhall IH AUID- ORCID: 0000-0003-4250-6459 AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Borthwick, S AU - Borthwick S AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Neves, E S AU - Neves ES AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Low, D AU - Low D AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Linster, M AU - Linster M AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Liang, B AU - Liang B AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Skiles, M AU - Skiles M AD - College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA. FAU - Jayakumar, J AU - Jayakumar J AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Han, H AU - Han H AD - Bioinformatics Institute, Agency for Science, Technology and Research, Singapore. FAU - Gunalan, V AU - Gunalan V AD - Bioinformatics Institute, Agency for Science, Technology and Research, Singapore. FAU - Lee, B P Y-H AU - Lee BPY AD - Durrell Institute of Conservation and Ecology, School of Anthropology and Conservation, University of Kent, Canterbury, UK. AD - National Parks Board, Singapore. FAU - Okahara, K AU - Okahara K AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Wang, L-F AU - Wang LF AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Maurer-Stroh, S AU - Maurer-Stroh S AD - Bioinformatics Institute, Agency for Science, Technology and Research, Singapore. AD - School of Biological Sciences, Nanyang Technological University, Singapore. FAU - Su, Y C F AU - Su YCF AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Smith, G J D AU - Smith GJD AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. AD - Duke Global Health Institute, Duke University, Durham, NC, USA. LA - eng SI - GENBANK/AY338732 SI - GENBANK/KX452686 PT - Journal Article DEP - 20160916 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 SB - IM MH - Animals MH - Betacoronavirus/genetics/*isolation & purification MH - Biological Evolution MH - Chiroptera/*virology MH - Coronavirus Infections/*veterinary/virology MH - Disease Reservoirs/*veterinary/virology MH - Ecology MH - Feces/virology MH - Female MH - High-Throughput Nucleotide Sequencing/veterinary MH - *Host-Pathogen Interactions MH - Male MH - Phylogeny MH - Reverse Transcriptase Polymerase Chain Reaction/veterinary MH - Singapore/epidemiology MH - Spatio-Temporal Analysis MH - Urine/virology PMC - PMC7159162 OTO - NOTNLM OT - disease ecology OT - distribution OT - evolution OT - virus diversity EDAT- 2016/09/18 06:00 MHDA- 2018/05/10 06:00 PMCR- 2020/04/15 CRDT- 2016/09/18 06:00 PHST- 2016/04/26 00:00 [received] PHST- 2016/09/18 06:00 [pubmed] PHST- 2018/05/10 06:00 [medline] PHST- 2016/09/18 06:00 [entrez] PHST- 2020/04/15 00:00 [pmc-release] AID - TBED12568 [pii] AID - 10.1111/tbed.12568 [doi] PST - ppublish SO - Transbound Emerg Dis. 2017 Dec;64(6):1790-1800. doi: 10.1111/tbed.12568. Epub 2016 Sep 16. PMID- 26656689 OWN - NLM STAT- MEDLINE DCOM- 20160609 LR - 20201209 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 90 IP - 4 DP - 2016 Feb 15 TI - Crystal Structure of Feline Infectious Peritonitis Virus Main Protease in Complex with Synergetic Dual Inhibitors. PG - 1910-7 LID - 10.1128/JVI.02685-15 [doi] AB - Coronaviruses (CoVs) can cause highly prevalent diseases in humans and animals. Feline infectious peritonitis virus (FIPV) belongs to the genus Alphacoronavirus, resulting in a lethal systemic granulomatous disease called feline infectious peritonitis (FIP), which is one of the most important fatal infectious diseases of cats worldwide. No specific vaccines or drugs have been approved to treat FIP. CoV main proteases (M(pro)s) play a pivotal role in viral transcription and replication, making them an ideal target for drug development. Here, we report the crystal structure of FIPV M(pro) in complex with dual inhibitors, a zinc ion and a Michael acceptor. The complex structure elaborates a unique mechanism of two distinct inhibitors synergizing to inactivate the protease, providing a structural basis to design novel antivirals and suggesting the potential to take advantage of zinc as an adjunct therapy against CoV-associated diseases. IMPORTANCE: Coronaviruses (CoVs) have the largest genome size among all RNA viruses. CoV infection causes various diseases in humans and animals, including severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS). No approved specific drugs or vaccinations are available to treat their infections. Here, we report a novel dual inhibition mechanism targeting CoV main protease (M(pro)) from feline infectious peritonitis virus (FIPV), which leads to lethal systemic granulomatous disease in cats. M(pro), conserved across all CoV genomes, is essential for viral replication and transcription. We demonstrated that zinc ion and a Michael acceptor-based peptidomimetic inhibitor synergistically inactivate FIPV M(pro). We also solved the structure of FIPV M(pro) complexed with two inhibitors, delineating the structural view of a dual inhibition mechanism. Our study provides new insight into the pharmaceutical strategy against CoV M(pro) through using zinc as an adjuvant therapy to enhance the efficacy of an irreversible peptidomimetic inhibitor. CI - Copyright © 2016, American Society for Microbiology. All Rights Reserved. FAU - Wang, Fenghua AU - Wang F AD - School of Life Sciences, Tianjin University, Tianjin, People's Republic of China. FAU - Chen, Cheng AU - Chen C AD - School of Life Sciences, Tianjin University, Tianjin, People's Republic of China Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, People's Republic of China. FAU - Liu, Xuemeng AU - Liu X AD - Institute of Ageing Research, School of Medicine, Hangzhou Normal University, Hangzhou, China. FAU - Yang, Kailin AU - Yang K AD - Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio, USA. FAU - Xu, Xiaoling AU - Xu X AD - Institute of Ageing Research, School of Medicine, Hangzhou Normal University, Hangzhou, China xuxl@hznu.edu.cn yanght@tju.edu.cn. FAU - Yang, Haitao AU - Yang H AD - School of Life Sciences, Tianjin University, Tianjin, People's Republic of China Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, People's Republic of China xuxl@hznu.edu.cn yanght@tju.edu.cn. LA - eng SI - PDB/2AMP PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151209 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Protease Inhibitors) RN - EC 3.4.22.- (Cysteine Endopeptidases) RN - EC 3.4.22.28 (Coronavirus 3C Proteases) RN - J41CSQ7QDS (Zinc) SB - IM MH - Amino Acid Sequence MH - Coronavirus 3C Proteases MH - Coronavirus, Feline/*enzymology MH - Crystallography, X-Ray MH - Cysteine Endopeptidases/*chemistry/*metabolism MH - Models, Molecular MH - Molecular Sequence Data MH - Protease Inhibitors/*chemistry/*metabolism MH - Protein Binding MH - Protein Conformation MH - Zinc/chemistry/metabolism PMC - PMC4734010 EDAT- 2015/12/15 06:00 MHDA- 2016/06/10 06:00 PMCR- 2016/07/28 CRDT- 2015/12/15 06:00 PHST- 2015/10/20 00:00 [received] PHST- 2015/11/24 00:00 [accepted] PHST- 2015/12/15 06:00 [entrez] PHST- 2015/12/15 06:00 [pubmed] PHST- 2016/06/10 06:00 [medline] PHST- 2016/07/28 00:00 [pmc-release] AID - JVI.02685-15 [pii] AID - 02685-15 [pii] AID - 10.1128/JVI.02685-15 [doi] PST - epublish SO - J Virol. 2015 Dec 9;90(4):1910-7. doi: 10.1128/JVI.02685-15. Print 2016 Feb 15. PMID- 30609457 OWN - NLM STAT- MEDLINE DCOM- 20190405 LR - 20190405 IS - 0047-1860 (Print) IS - 0047-1860 (Linking) VI - 64 IP - 9 DP - 2016 Sep TI - [Middle East Respiratory Syndrome (MERS)]. PG - 1044-1051 AB - Middle East respiratory syndrome (MERS) is an emerging infectious disease of growing global importance, which has caused severe acute respiratory disease in more than 1,700 people, resulting in almost 600 deaths. MERS is caused by a novel betacoronavirus (MERS-CoV). All cases of MERS have been linked through travel to or residence in countries in or near the Arabian Peninsula. Dromedary camels are considered natu- ral reservoirs for MERS-CoV. MERS-CoV is mainly transmitted from infected dromedary camels to human beings, and it is transmitted among human beings by droplets, contact, and perhaps airborne spread. Both community-acquired and hospital-acquired cases have been reported with little human-to-human transmis- sion reported in the community. The largest known outbreak of MERS outside the Arabian Peninsula oc- curred in the Republic of Korea in 2015, with 186 cases. The outbreak was associated with a traveler re- turning from the Arabian Peninsula. Clinical features of MERS range from asymptomatic or mild disease to acute respiratory distress syndrome and multi-organ failure resulting in death, especially in individuals with underlying comorbidities. MERS is suspected in the presence of febrile acute respiratory illness and close contact with MERS-CoV, and can be confirmed by the detection of viral nucleic acid through RT-PCR or se- rology. No specific drug treatment exists for MERS; however, the neutralizing antibodies, ribavirin and interferon have been shown to be potentially useful anti-MERS-CoV drugs. Rigorous infection prevention and control measures with droplet and contact precautions are crucial to prevent the spread in health-care facilities. [Review]. FAU - Iinuma, Yoshitsugu AU - Iinuma Y LA - jpn PT - Journal Article PT - Review PL - Japan TA - Rinsho Byori JT - Rinsho byori. The Japanese journal of clinical pathology JID - 2984781R SB - IM MH - Animals MH - Coronavirus/chemistry/physiology MH - *Coronavirus Infections/diagnosis/epidemiology/prevention & control/transmission MH - Humans MH - Vaccination MH - World Health Organization EDAT- 2016/09/01 00:00 MHDA- 2016/09/01 00:01 CRDT- 2019/01/05 06:00 PHST- 2019/01/05 06:00 [entrez] PHST- 2016/09/01 00:00 [pubmed] PHST- 2016/09/01 00:01 [medline] PST - ppublish SO - Rinsho Byori. 2016 Sep;64(9):1044-1051. PMID- 27784629 OWN - NLM STAT- MEDLINE DCOM- 20171212 LR - 20210119 IS - 1872-7492 (Electronic) IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 227 DP - 2017 Jan 2 TI - Efficient priming of CD4 T cells by Langerin-expressing dendritic cells targeted with porcine epidemic diarrhea virus spike protein domains in pigs. PG - 212-219 LID - S0168-1702(16)30496-8 [pii] LID - 10.1016/j.virusres.2016.10.007 [doi] AB - Porcine epidemic diarrhea virus (PEDV) first emerged in the United States in 2013 causing high mortality and morbidity in neonatal piglets with immense economic losses to the swine industry. PEDV is an alpha-coronavirus replicating primarily in porcine intestinal cells. PEDV vaccines are available in Asia and Europe, and conditionally-licensed vaccines recently became available in the United States but the efficacies of these vaccines in eliminating PEDV from swine populations are questionable. In this study, the immunogenicity of a subunit vaccine based on the spike protein of PEDV, which was directly targeted to porcine dendritic cells (DCs) expressing Langerin, was assessed. The PEDV S antigen was delivered to the dendritic cells through a single-chain antibody specific to Langerin and the targeted cells were stimulated with cholera toxin adjuvant. This approach, known as "dendritic cell targeting," greatly improved PEDV S antigen-specific T cell interferon-γ responses in the CD4(pos)CD8(pos) T cell compartment in pigs as early as 7days upon transdermal administration. When the vaccine protein was targeted to Langerin(pos) DCs systemically through intramuscular vaccination, it induced higher serum IgG and IgA responses in pigs, though these responses require a booster dose, and the magnitude of T cell responses were lower as compared to transdermal vaccination. We conclude that PEDV spike protein domains targeting Langerin-expressing dendritic cells significantly increased CD4 T cell immune responses in pigs. The results indicate that the immunogenicity of protein subunit vaccines can be greatly enhanced by direct targeting of the vaccine antigens to desirable dendritic cell subsets in pigs. CI - Copyright © 2016 Elsevier B.V. All rights reserved. FAU - Subramaniam, Sakthivel AU - Subramaniam S AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Cao, Dianjun AU - Cao D AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Tian, Debin AU - Tian D AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Cao, Qian M AU - Cao QM AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Overend, Christopher AU - Overend C AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Yugo, Danielle M AU - Yugo DM AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Matzinger, Shannon R AU - Matzinger SR AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Rogers, Adam J AU - Rogers AJ AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Heffron, C Lynn AU - Heffron CL AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Catanzaro, Nicholas AU - Catanzaro N AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Kenney, Scott P AU - Kenney SP AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. FAU - Opriessnig, Tanja AU - Opriessnig T AD - The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, EH25 9RG Scotland, UK. FAU - Huang, Yao-Wei AU - Huang YW AD - Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Labarque, Geoffrey AU - Labarque G AD - Elanco Biological R&D, Eli Lilly and Company, Greenfield, IN 46140, USA. FAU - Wu, Stephen Q AU - Wu SQ AD - Elanco Biological R&D, Eli Lilly and Company, Greenfield, IN 46140, USA. FAU - Meng, Xiang-Jin AU - Meng XJ AD - Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. Electronic address: xjmeng@vt.edu. LA - eng PT - Journal Article DEP - 20161023 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (Antigens, Viral) RN - 0 (Immunoglobulin A) RN - 0 (Single-Chain Antibodies) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Vaccines, Subunit) RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Antibodies, Neutralizing/immunology MH - Antibodies, Viral/immunology MH - Antibody Specificity/immunology MH - Antigens, Viral/immunology MH - CD4-Positive T-Lymphocytes/*immunology MH - CHO Cells MH - Chlorocebus aethiops MH - Coronavirus Infections/veterinary MH - Cricetulus MH - Dendritic Cells/*immunology/*metabolism MH - Immunization MH - Immunoglobulin A/immunology MH - Intestinal Mucosa/immunology MH - Porcine epidemic diarrhea virus/*immunology MH - Protein Domains/*immunology MH - Single-Chain Antibodies/immunology MH - Spike Glycoprotein, Coronavirus/chemistry/*immunology MH - Swine MH - Swine Diseases/immunology/prevention & control/virology MH - T-Lymphocyte Subsets/immunology/metabolism MH - Vaccines, Subunit/immunology MH - Vero Cells MH - Viral Vaccines/immunology PMC - PMC7114527 OTO - NOTNLM OT - Dendritic cell targeting OT - Porcine Langerin OT - Porcine epidemic diarrhea virus (PEDV) OT - Subunit vaccine OT - T cell immunity EDAT- 2016/11/05 06:00 MHDA- 2017/12/13 06:00 PMCR- 2016/10/23 CRDT- 2016/11/07 06:00 PHST- 2016/08/09 00:00 [received] PHST- 2016/10/13 00:00 [revised] PHST- 2016/10/14 00:00 [accepted] PHST- 2016/11/05 06:00 [pubmed] PHST- 2017/12/13 06:00 [medline] PHST- 2016/11/07 06:00 [entrez] PHST- 2016/10/23 00:00 [pmc-release] AID - S0168-1702(16)30496-8 [pii] AID - 10.1016/j.virusres.2016.10.007 [doi] PST - ppublish SO - Virus Res. 2017 Jan 2;227:212-219. doi: 10.1016/j.virusres.2016.10.007. Epub 2016 Oct 23. PMID- 25706132 OWN - NLM STAT- MEDLINE DCOM- 20151201 LR - 20240714 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 10 IP - 2 DP - 2015 TI - Analysis of cathepsin and furin proteolytic enzymes involved in viral fusion protein activation in cells of the bat reservoir host. PG - e0115736 LID - 10.1371/journal.pone.0115736 [doi] LID - e0115736 AB - Bats of different species play a major role in the emergence and transmission of highly pathogenic viruses including Ebola virus, SARS-like coronavirus and the henipaviruses. These viruses require proteolytic activation of surface envelope glycoproteins needed for entry, and cellular cathepsins have been shown to be involved in proteolysis of glycoproteins from these distinct virus families. Very little is currently known about the available proteases in bats. To determine whether the utilization of cathepsins by bat-borne viruses is related to the nature of proteases in their natural hosts, we examined proteolytic processing of several viral fusion proteins in cells derived from two fruit bat species, Pteropus alecto and Rousettus aegyptiacus. Our work shows that fruit bat cells have homologs of cathepsin and furin proteases capable of cleaving and activating both the cathepsin-dependent Hendra virus F and the furin-dependent parainfluenza virus 5 F proteins. Sequence analysis comparing Pteropus alecto furin and cathepsin L to proteases from other mammalian species showed a high degree of conservation; however significant amino acid variation occurs at the C-terminus of Pteropus alecto furin. Further analysis of furin-like proteases from fruit bats revealed that these proteases are catalytically active and resemble other mammalian furins in their response to a potent furin inhibitor. However, kinetic analysis suggests that differences may exist in the cellular localization of furin between different species. Collectively, these results indicate that the unusual role of cathepsin proteases in the life cycle of bat-borne viruses is not due to the lack of active furin-like proteases in these natural reservoir species; however, differences may exist between furin proteases present in fruit bats compared to furins in other mammalian species, and these differences may impact protease usage for viral glycoprotein processing. FAU - El Najjar, Farah AU - El Najjar F AD - Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, United States of America. FAU - Lampe, Levi AU - Lampe L AD - Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, United States of America. FAU - Baker, Michelle L AU - Baker ML AD - CSIRO Australian Animal Health Laboratory, East Geelong, Victoria, Australia. FAU - Wang, Lin-Fa AU - Wang LF AD - CSIRO Australian Animal Health Laboratory, East Geelong, Victoria, Australia; Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Singapore, Singapore. FAU - Dutch, Rebecca Ellis AU - Dutch RE AD - Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, United States of America. LA - eng GR - P20 RR020171/RR/NCRR NIH HHS/United States GR - R01 AI051517/AI/NIAID NIH HHS/United States GR - R01AI051517/AI/NIAID NIH HHS/United States GR - R56 AI051517/AI/NIAID NIH HHS/United States GR - U54 AI057157/AI/NIAID NIH HHS/United States GR - 2P20 RR020171/RR/NCRR NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20150223 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Viral Fusion Proteins) RN - 0 (Viral Proteins) RN - EC 3.4.- (Cathepsins) RN - EC 3.4.- (Peptide Hydrolases) RN - EC 3.4.21.75 (Furin) SB - IM MH - Animals MH - Cathepsins/*metabolism MH - Cell Line MH - Chiroptera/*virology MH - Chlorocebus aethiops MH - Cricetinae MH - Furin/*metabolism MH - Peptide Hydrolases/*metabolism MH - Vero Cells MH - Viral Fusion Proteins/*metabolism MH - Viral Proteins/*metabolism PMC - PMC4338073 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2015/02/24 06:00 MHDA- 2015/12/15 06:00 PMCR- 2015/02/23 CRDT- 2015/02/24 06:00 PHST- 2014/10/24 00:00 [received] PHST- 2014/11/18 00:00 [accepted] PHST- 2015/02/24 06:00 [entrez] PHST- 2015/02/24 06:00 [pubmed] PHST- 2015/12/15 06:00 [medline] PHST- 2015/02/23 00:00 [pmc-release] AID - PONE-D-14-47612 [pii] AID - 10.1371/journal.pone.0115736 [doi] PST - epublish SO - PLoS One. 2015 Feb 23;10(2):e0115736. doi: 10.1371/journal.pone.0115736. eCollection 2015. PMID- 28854955 OWN - NLM STAT- MEDLINE DCOM- 20180118 LR - 20191210 IS - 1297-9716 (Electronic) IS - 0928-4249 (Print) IS - 0928-4249 (Linking) VI - 48 IP - 1 DP - 2017 Aug 30 TI - Identification of two mutation sites in spike and envelope proteins mediating optimal cellular infection of porcine epidemic diarrhea virus from different pathways. PG - 44 LID - 10.1186/s13567-017-0449-y [doi] LID - 44 AB - Entry of the α-coronavirus porcine epidemic diarrhea virus (PEDV) requires specific proteases to activate spike (S) protein for the membrane fusion of the virion to the host cell following receptor binding. Herein, PEDV isolate 85-7 could proliferate and induce cell-cell fusion in a trypsin independent manner on Vero cells, and eight homologous mutation strains were screened by continuous proliferation in the absence of trypsin on Vero cells. According to the whole genome sequence comparative analysis, we identified four major variations located in nonstructural protein 2, S, open reading frame 3, and envelope (E) genes, respectively. Comparative analyses of their genomic variations and proliferation characteristics identified a single mutation within the S2' cleavage site between C30 and C40 mutants: the substitution of conserved arginine (R) by a glycine (G) (R895G). This change resulted in weaker cell-cell fusion, smaller plaque morphology, higher virus titer and serious microfilament condensation. Further analysis confirmed that this mutation was responsible for optimal cell-adaptation, but not the determinant for trypsin-dependent entry of PEDV. Otherwise, a novel variation (16-20 aa deletion and an L25P mutation) in the transmembrane domain of the E protein affected multiple infection processes, including up-regulation of the production of the ER stress indicator GRP78, improving the expression of pro-inflammatory cytokines IL-6 and IL-8, and promoting apoptosis. The results of this study provide a better understanding of the potential mechanisms of viral functional proteins in PEDV replication, infection, and fitness. FAU - Sun, Min AU - Sun M AD - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. FAU - Ma, Jiale AU - Ma J AD - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. FAU - Yu, Zeyanqiu AU - Yu Z AD - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. FAU - Pan, Zihao AU - Pan Z AD - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. FAU - Lu, Chengping AU - Lu C AD - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. FAU - Yao, Huochun AU - Yao H AUID- ORCID: 0000-0002-3749-8557 AD - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China. yaohch@njau.edu.cn. LA - eng PT - Journal Article DEP - 20170830 PL - England TA - Vet Res JT - Veterinary research JID - 9309551 RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Viral Envelope Proteins) SB - IM MH - Animals MH - Chlorocebus aethiops MH - Coronavirus Infections/*veterinary/virology MH - Mutation/genetics MH - Porcine epidemic diarrhea virus/genetics/*pathogenicity/physiology MH - Spike Glycoprotein, Coronavirus/*genetics/physiology MH - Swine MH - Swine Diseases/*virology MH - Vero Cells MH - Viral Envelope Proteins/*genetics/physiology PMC - PMC5577753 EDAT- 2017/09/01 06:00 MHDA- 2018/01/19 06:00 PMCR- 2017/08/30 CRDT- 2017/09/01 06:00 PHST- 2017/01/30 00:00 [received] PHST- 2017/05/31 00:00 [accepted] PHST- 2017/09/01 06:00 [entrez] PHST- 2017/09/01 06:00 [pubmed] PHST- 2018/01/19 06:00 [medline] PHST- 2017/08/30 00:00 [pmc-release] AID - 10.1186/s13567-017-0449-y [pii] AID - 449 [pii] AID - 10.1186/s13567-017-0449-y [doi] PST - epublish SO - Vet Res. 2017 Aug 30;48(1):44. doi: 10.1186/s13567-017-0449-y. PMID- 29802920 OWN - NLM STAT- MEDLINE DCOM- 20181108 LR - 20240329 IS - 1879-0003 (Electronic) IS - 0141-8130 (Print) IS - 0141-8130 (Linking) VI - 117 DP - 2018 Oct 1 TI - Characterization of the interaction between recombinant porcine aminopeptidase N and spike glycoprotein of porcine epidemic diarrhea virus. PG - 704-712 LID - S0141-8130(18)30530-0 [pii] LID - 10.1016/j.ijbiomac.2018.05.167 [doi] AB - Porcine epidemic diarrhea (PED) has caused huge economic losses to the global pork industry. Infection by its causative agent PED virus (PEDV), an Alpha-coronavirus, was previously proven to be mediated by its spike (S) glycoprotein and a cellular receptor porcine aminopeptidase N (pAPN). Interestingly, some recent studies have indicated that pAPN is not a functional receptor for PEDV. To date, there is a lack of a direct evidence for the interaction between pAPN and PEDV S protein in vitro. Here, we prepared pAPN ectodomain and the truncated variants of PEDV S protein in Drosophila S2 cells. These recombinant proteins were homogeneous after purification by metal-affinity and size-exclusion chromatography. We then assayed the purified target proteins through immunogenicity tests, PEDV binding interference assays, circular dichroism (CD) measurements, pAPN activity assay and structural determination, demonstrating that they were biologically functional. Finally, we characterized their interactions by gel filtration chromatography, native-polyacrylamide gel electrophoresis (PAGE) and surface plasmon resonance (SPR) analyses. The results showed that their affinities were too low to form complexes, which suggest that pAPN may be controversial as the genuine receptor for PEDV. Therefore, further research needs to be carried out to elucidate the interaction between PEDV and its genuine receptor. CI - Copyright © 2018. Published by Elsevier B.V. FAU - Sun, Yan-Gang AU - Sun YG AD - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China. FAU - Li, Rui AU - Li R AD - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China. FAU - Jiang, Longguang AU - Jiang L AD - College of Chemistry, Fuzhou University, Fuzhou 350116, Fujian, China. FAU - Qiao, Songlin AU - Qiao S AD - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China. FAU - Zhi, Yubao AU - Zhi Y AD - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China. FAU - Chen, Xin-Xin AU - Chen XX AD - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China. FAU - Xie, Sha AU - Xie S AD - College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shanxi, China. FAU - Wu, Jiawei AU - Wu J AD - GE Healthcare Life Sciences, Beijing 100176, China. FAU - Li, Xuewu AU - Li X AD - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China. FAU - Deng, Ruiguang AU - Deng R AD - Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China. FAU - Zhang, Gaiping AU - Zhang G AD - College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shanxi, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan, China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, Jiangsu, China. Electronic address: zhanggaiping2003@163.com. LA - eng PT - Journal Article DEP - 20180524 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - 0 (Recombinant Proteins) RN - 0 (Spike Glycoprotein, Coronavirus) RN - EC 3.4.11.2 (CD13 Antigens) SB - IM MH - Animals MH - CD13 Antigens/*chemistry/genetics MH - Chlorocebus aethiops MH - Coronavirus Infections/*genetics/veterinary/virology MH - Host-Pathogen Interactions/genetics MH - Porcine epidemic diarrhea virus/chemistry/*genetics MH - Recombinant Proteins/chemistry/genetics MH - Spike Glycoprotein, Coronavirus/chemistry/*genetics MH - Swine MH - Vero Cells PMC - PMC7112428 OTO - NOTNLM OT - Interaction OT - Porcine aminopeptidase N OT - Porcine epidemic diarrhea virus EDAT- 2018/05/29 06:00 MHDA- 2018/11/09 06:00 PMCR- 2018/05/24 CRDT- 2018/05/27 06:00 PHST- 2018/02/10 00:00 [received] PHST- 2018/05/11 00:00 [revised] PHST- 2018/05/22 00:00 [accepted] PHST- 2018/05/29 06:00 [pubmed] PHST- 2018/11/09 06:00 [medline] PHST- 2018/05/27 06:00 [entrez] PHST- 2018/05/24 00:00 [pmc-release] AID - S0141-8130(18)30530-0 [pii] AID - 10.1016/j.ijbiomac.2018.05.167 [doi] PST - ppublish SO - Int J Biol Macromol. 2018 Oct 1;117:704-712. doi: 10.1016/j.ijbiomac.2018.05.167. Epub 2018 May 24. PMID- 28377531 OWN - NLM STAT- MEDLINE DCOM- 20180108 LR - 20191227 IS - 2150-7511 (Electronic) VI - 8 IP - 2 DP - 2017 Apr 4 TI - Further Evidence for Bats as the Evolutionary Source of Middle East Respiratory Syndrome Coronavirus. LID - 10.1128/mBio.00373-17 [doi] LID - e00373-17 AB - The evolutionary origins of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) are unknown. Current evidence suggests that insectivorous bats are likely to be the original source, as several 2c CoVs have been described from various species in the family Vespertilionidae Here, we describe a MERS-like CoV identified from a Pipistrellus cf. hesperidus bat sampled in Uganda (strain PREDICT/PDF-2180), further supporting the hypothesis that bats are the evolutionary source of MERS-CoV. Phylogenetic analysis showed that PREDICT/PDF-2180 is closely related to MERS-CoV across much of its genome, consistent with a common ancestry; however, the spike protein was highly divergent (46% amino acid identity), suggesting that the two viruses may have different receptor binding properties. Indeed, several amino acid substitutions were identified in key binding residues that were predicted to block PREDICT/PDF-2180 from attaching to the MERS-CoV DPP4 receptor. To experimentally test this hypothesis, an infectious MERS-CoV clone expressing the PREDICT/PDF-2180 spike protein was generated. Recombinant viruses derived from the clone were replication competent but unable to spread and establish new infections in Vero cells or primary human airway epithelial cells. Our findings suggest that PREDICT/PDF-2180 is unlikely to pose a zoonotic threat. Recombination in the S1 subunit of the spike gene was identified as the primary mechanism driving variation in the spike phenotype and was likely one of the critical steps in the evolution and emergence of MERS-CoV in humans.IMPORTANCE Global surveillance efforts for undiscovered viruses are an important component of pandemic prevention initiatives. These surveys can be useful for finding novel viruses and for gaining insights into the ecological and evolutionary factors driving viral diversity; however, finding a viral sequence is not sufficient to determine whether it can infect people (i.e., poses a zoonotic threat). Here, we investigated the specific zoonotic risk of a MERS-like coronavirus (PREDICT/PDF-2180) identified in a bat from Uganda and showed that, despite being closely related to MERS-CoV, it is unlikely to pose a threat to humans. We suggest that this approach constitutes an appropriate strategy for beginning to determine the zoonotic potential of wildlife viruses. By showing that PREDICT/PDF-2180 does not infect cells that express the functional receptor for MERS-CoV, we further show that recombination was likely to be the critical step that allowed MERS to emerge in humans. CI - Copyright © 2017 Anthony et al. FAU - Anthony, S J AU - Anthony SJ AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA sja2127@cumc.columbia.edu. AD - Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, USA. AD - EcoHealth Alliance, New York, New York, USA. FAU - Gilardi, K AU - Gilardi K AD - One Health Institute and Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California, Davis, California, USA. FAU - Menachery, V D AU - Menachery VD AD - Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. FAU - Goldstein, T AU - Goldstein T AD - One Health Institute and Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California, Davis, California, USA. FAU - Ssebide, B AU - Ssebide B AD - Gorilla Doctors, c/o MGVP, Inc., Davis, California, USA. FAU - Mbabazi, R AU - Mbabazi R AD - Gorilla Doctors, c/o MGVP, Inc., Davis, California, USA. FAU - Navarrete-Macias, I AU - Navarrete-Macias I AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA. FAU - Liang, E AU - Liang E AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA. AD - EcoHealth Alliance, New York, New York, USA. FAU - Wells, H AU - Wells H AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA. FAU - Hicks, A AU - Hicks A AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA. FAU - Petrosov, A AU - Petrosov A AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA. FAU - Byarugaba, D K AU - Byarugaba DK AD - Makerere University Walter Reed Project, Kampala, Uganda. AD - Makerere University, College of Veterinary Medicine, Kampala, Uganda. FAU - Debbink, K AU - Debbink K AD - Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. FAU - Dinnon, K H AU - Dinnon KH AD - Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. FAU - Scobey, T AU - Scobey T AD - Department of Epidemiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. FAU - Randell, S H AU - Randell SH AD - Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. FAU - Yount, B L AU - Yount BL AD - Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. FAU - Cranfield, M AU - Cranfield M AD - One Health Institute and Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California, Davis, California, USA. AD - Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. FAU - Johnson, C K AU - Johnson CK AD - One Health Institute and Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California, Davis, California, USA. FAU - Baric, R S AU - Baric RS AD - Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA. FAU - Lipkin, W I AU - Lipkin WI AD - Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA. AD - Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, USA. FAU - Mazet, J A K AU - Mazet JA AD - One Health Institute and Karen C. Drayer Wildlife Health Center, School of Veterinary Medicine, University of California, Davis, California, USA. LA - eng GR - R01 AI110700/AI/NIAID NIH HHS/United States GR - U19 AI109761/AI/NIAID NIH HHS/United States GR - R00 AG049092/AG/NIA NIH HHS/United States GR - K99 AG049092/AG/NIA NIH HHS/United States GR - P30 DK065988/DK/NIDDK NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20170404 PL - United States TA - mBio JT - mBio JID - 101519231 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Animals MH - Chiroptera/*virology MH - Evolution, Molecular MH - Genome, Viral MH - Middle East Respiratory Syndrome Coronavirus/*classification/genetics/*isolation & purification/physiology MH - *Phylogeny MH - Receptors, Virus/metabolism MH - Spike Glycoprotein, Coronavirus/genetics/metabolism MH - Synteny MH - Uganda MH - *Virus Attachment PMC - PMC5380844 OTO - NOTNLM OT - MERS coronavirus OT - Uganda OT - bat OT - spike OT - zoonoses EDAT- 2017/04/06 06:00 MHDA- 2018/01/09 06:00 PMCR- 2017/04/04 CRDT- 2017/04/06 06:00 PHST- 2017/04/06 06:00 [entrez] PHST- 2017/04/06 06:00 [pubmed] PHST- 2018/01/09 06:00 [medline] PHST- 2017/04/04 00:00 [pmc-release] AID - mBio.00373-17 [pii] AID - mBio00373-17 [pii] AID - 10.1128/mBio.00373-17 [doi] PST - epublish SO - mBio. 2017 Apr 4;8(2):e00373-17. doi: 10.1128/mBio.00373-17. PMID- 28684708 OWN - NLM STAT- MEDLINE DCOM- 20180312 LR - 20240326 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 9 IP - 7 DP - 2017 Jul 6 TI - Porcine Epidemic Diarrhea in Europe: In-Detail Analyses of Disease Dynamics and Molecular Epidemiology. LID - 10.3390/v9070177 [doi] LID - 177 AB - Porcine epidemic diarrhea (PED) is an acute and highly contagious enteric disease of swine caused by the eponymous virus (PEDV) which belongs to the genus Alphacoronavirus within the Coronaviridae virus family. Following the disastrous outbreaks in Asia and the United States, PEDV has been detected also in Europe. In order to better understand the overall situation, the molecular epidemiology, and factors that might influence the most variable disease impact; 40 samples from swine feces were collected from different PED outbreaks in Germany and other European countries and sequenced by shot-gun next-generation sequencing. A total of 38 new PEDV complete coding sequences were generated. When compared on a global scale, all investigated sequences from Central and South-Eastern Europe formed a rather homogeneous PEDV S INDEL cluster, suggesting a recent re-introduction. However, in-detail analyses revealed two new clusters and putative ancestor strains. Based on the available background data, correlations between clusters and location, farm type or clinical presentation could not be established. Additionally, the impact of secondary infections was explored using the metagenomic data sets. While several coinfections were observed, no correlation was found with disease courses. However, in addition to the PEDV genomes, ten complete viral coding sequences from nine different data sets were reconstructed each representing new virus strains. In detail, three pasivirus A strains, two astroviruses, a porcine sapelovirus, a kobuvirus, a porcine torovirus, a posavirus, and an enterobacteria phage were almost fully sequenced. FAU - Hanke, Dennis AU - Hanke D AD - Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany. dennis.hanke@fli.de. FAU - Pohlmann, Anne AU - Pohlmann A AD - Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany. anne.pohlmann@fli.de. FAU - Sauter-Louis, Carola AU - Sauter-Louis C AD - Friedrich-Loeffler-Institut, Institute of Epidemiology, D-17493 Greifswald-Insel Riems, Germany. carola.sauter-louis@fli.de. FAU - Höper, Dirk AU - Höper D AD - Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany. dirk.hoeper@fli.de. FAU - Stadler, Julia AU - Stadler J AD - Clinic for Swine, Ludwig-Maximilians-University Munich, D-85764 Oberschleissheim, Germany. J.Stadler@med.vetmed.uni-muenchen.de. FAU - Ritzmann, Mathias AU - Ritzmann M AD - Clinic for Swine, Ludwig-Maximilians-University Munich, D-85764 Oberschleissheim, Germany. Ritzmann@med.vetmed.uni-muenchen.de. FAU - Steinrigl, Adi AU - Steinrigl A AD - Österreichische Agentur für Gesundheit und Ernährungssicherheit GmbH, A-2340 Mödling, Austria. adi.steinrigl@ages.at. FAU - Schwarz, Bernd-Andreas AU - Schwarz BA AD - Vaxxinova GmbH, Standort Leipzig, D-04103 Leipzig, Germany. bernd-andreas.schwarz@vaxxinova.com. FAU - Akimkin, Valerij AU - Akimkin V AD - Chemisches und Veterinäruntersuchungsamt Stuttgart, Fellbach, D-70736 Fellbach, Germany, Valerij.Akimkin@cvuas.bwl.de. Valerij.Akimkin@cvuas.bwl.de. FAU - Fux, Robert AU - Fux R AD - Institute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-University Munich, D-80539 Munich, Germany. robert.fux@micro.vetmed.uni-muenchen.de. FAU - Blome, Sandra AU - Blome S AD - Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany. sandra.blome@fli.de. FAU - Beer, Martin AU - Beer M AD - Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, D-17493 Greifswald-Insel Riems, Germany. martin.beer@fli.de. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170706 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Cluster Analysis MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Diarrhea/epidemiology/*veterinary/virology MH - *Disease Outbreaks MH - Europe/epidemiology MH - Feces/virology MH - Molecular Epidemiology MH - Phylogeny MH - Porcine epidemic diarrhea virus/*classification/*genetics/isolation & purification MH - RNA, Viral/genetics MH - Sequence Analysis, DNA MH - Swine MH - Swine Diseases/*epidemiology/*virology PMC - PMC5537669 OTO - NOTNLM OT - metagenome OT - phylogenetic analyses OT - porcine epidemic diarrhea OT - porcine epidemic diarrhea virus COIS- The authors declare no conflict of interest. EDAT- 2017/07/08 06:00 MHDA- 2018/03/13 06:00 PMCR- 2017/07/01 CRDT- 2017/07/08 06:00 PHST- 2017/05/24 00:00 [received] PHST- 2017/06/29 00:00 [revised] PHST- 2017/06/30 00:00 [accepted] PHST- 2017/07/08 06:00 [entrez] PHST- 2017/07/08 06:00 [pubmed] PHST- 2018/03/13 06:00 [medline] PHST- 2017/07/01 00:00 [pmc-release] AID - v9070177 [pii] AID - viruses-09-00177 [pii] AID - 10.3390/v9070177 [doi] PST - epublish SO - Viruses. 2017 Jul 6;9(7):177. doi: 10.3390/v9070177. PMID- 30679277 OWN - NLM STAT- MEDLINE DCOM- 20190624 LR - 20200225 IS - 1091-6490 (Electronic) IS - 0027-8424 (Print) IS - 0027-8424 (Linking) VI - 116 IP - 7 DP - 2019 Feb 12 TI - Human coronaviruses OC43 and HKU1 bind to 9-O-acetylated sialic acids via a conserved receptor-binding site in spike protein domain A. PG - 2681-2690 LID - 10.1073/pnas.1809667116 [doi] AB - Human betacoronaviruses OC43 and HKU1 are endemic respiratory pathogens and, while related, originated from independent zoonotic introductions. OC43 is in fact a host-range variant of the species Betacoronavirus-1, and more closely related to bovine coronavirus (BCoV)-its presumptive ancestor-and porcine hemagglutinating encephalomyelitis virus (PHEV). The β1-coronaviruses (β1CoVs) and HKU1 employ glycan-based receptors carrying 9-O-acetylated sialic acid (9-O-Ac-Sia). Receptor binding is mediated by spike protein S, the main determinant of coronavirus host specificity. For BCoV, a crystal structure for the receptor-binding domain S1(A) is available and for HKU1 a cryoelectron microscopy structure of the complete S ectodomain. However, the location of the receptor-binding site (RBS), arguably the single-most important piece of information, is unknown. Here we solved the 3.0-Å crystal structure of PHEV S1(A) We then took a comparative structural analysis approach to map the β1CoV S RBS, using the general design of 9-O-Ac-Sia-binding sites as blueprint, backed-up by automated ligand docking, structure-guided mutagenesis of OC43, BCoV, and PHEV S1(A), and infectivity assays with BCoV-S-pseudotyped vesicular stomatitis viruses. The RBS is not exclusive to OC43 and related animal viruses, but is apparently conserved and functional also in HKU1 S1(A) The binding affinity of the HKU1 S RBS toward short sialoglycans is significantly lower than that of OC43, which we attribute to differences in local architecture and accessibility, and which may be indicative for differences between the two viruses in receptor fine-specificity. Our findings challenge reports that would map the OC43 RBS elsewhere in S1(A) and that of HKU1 in domain S1(B). FAU - Hulswit, Ruben J G AU - Hulswit RJG AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - Lang, Yifei AU - Lang Y AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - Bakkers, Mark J G AU - Bakkers MJG AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - Li, Wentao AU - Li W AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - Li, Zeshi AU - Li Z AD - Department of Chemical Biology and Drug Discovery and Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CG Utrecht, The Netherlands. FAU - Schouten, Arie AU - Schouten A AD - Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Faculty of Sciences, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - Ophorst, Bram AU - Ophorst B AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - van Kuppeveld, Frank J M AU - van Kuppeveld FJM AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - Boons, Geert-Jan AU - Boons GJ AD - Department of Chemical Biology and Drug Discovery and Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CG Utrecht, The Netherlands. AD - Department of Chemistry, University of Georgia, Athens, GA 30602. AD - Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602. FAU - Bosch, Berend-Jan AU - Bosch BJ AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - Huizinga, Eric G AU - Huizinga EG AUID- ORCID: 0000-0001-6928-8426 AD - Crystal and Structural Chemistry, Bijvoet Center for Biomolecular Research, Faculty of Sciences, Utrecht University, 3584 CH Utrecht, The Netherlands. FAU - de Groot, Raoul J AU - de Groot RJ AD - Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CH Utrecht, The Netherlands; r.j.degroot@uu.nl. LA - eng SI - PDB/6QFY SI - GENBANK/MG999832-35 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190124 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) RN - GZP2782OP0 (N-Acetylneuraminic Acid) SB - IM MH - Acetylation MH - Animals MH - Binding Sites MH - Coronavirus OC43, Human/*physiology MH - Humans MH - *Membrane Fusion MH - N-Acetylneuraminic Acid/*metabolism MH - Rats MH - Receptors, Virus/chemistry/*metabolism MH - Spike Glycoprotein, Coronavirus/*metabolism PMC - PMC6377473 OTO - NOTNLM OT - 9-O-acetylated sialic acid OT - HKU1 OT - OC43 OT - coronavirus OT - spike COIS- The authors declare no conflict of interest. EDAT- 2019/01/27 06:00 MHDA- 2019/06/25 06:00 PMCR- 2019/01/24 CRDT- 2019/01/26 06:00 PHST- 2019/01/27 06:00 [pubmed] PHST- 2019/06/25 06:00 [medline] PHST- 2019/01/26 06:00 [entrez] PHST- 2019/01/24 00:00 [pmc-release] AID - 1809667116 [pii] AID - 201809667 [pii] AID - 10.1073/pnas.1809667116 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2019 Feb 12;116(7):2681-2690. doi: 10.1073/pnas.1809667116. Epub 2019 Jan 24. PMID- 25053787 OWN - NLM STAT- MEDLINE DCOM- 20150512 LR - 20211021 IS - 2150-7511 (Electronic) VI - 5 IP - 4 DP - 2014 Jul 22 TI - Detection of the Middle East respiratory syndrome coronavirus genome in an air sample originating from a camel barn owned by an infected patient. PG - e01450-14 LID - 10.1128/mBio.01450-14 [doi] LID - e01450-14 AB - Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel betacoronavirus that has been circulating in the Arabian Peninsula since 2012 and causing severe respiratory infections in humans. While bats were suggested to be involved in human MERS-CoV infections, a direct link between bats and MERS-CoV is uncertain. On the other hand, serological and virological data suggest dromedary camels as the potential animal reservoirs of MERS-CoV. Recently, we isolated MERS-CoV from a camel and its infected owner and provided evidence for the direct transmission of MERS-CoV from the infected camel to the patient. Here, we extend this work and show that identical MERS-CoV RNA fragments were detected in an air sample collected from the same barn that sheltered the infected camel in our previous study. These data indicate that the virus was circulating in this farm concurrently with its detection in the camel and in the patient, which warrants further investigations for the possible airborne transmission of MERS-CoV. Importance: This work clearly highlights the importance of continuous surveillance and infection control measures to control the global public threat of MERS-CoV. While current MERS-CoV transmission appears to be limited, we advise minimal contact with camels, especially for immunocompromised individuals, and the use of appropriate health, safety, and infection prevention and control measures when dealing with infected patients. Also, detailed clinical histories of any MERS-CoV cases with epidemiological and laboratory investigations carried out for any animal exposure must be considered to identify any animal source. CI - Copyright © 2014 Azhar et al. FAU - Azhar, Esam I AU - Azhar EI AD - eazhar@kau.edu.sa. FAU - Hashem, Anwar M AU - Hashem AM FAU - El-Kafrawy, Sherif A AU - El-Kafrawy SA AD - Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. FAU - Sohrab, Sayed Sartaj AU - Sohrab SS AD - Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. FAU - Aburizaiza, Asad S AU - Aburizaiza AS AD - Environmental Science Department, Faculty of Metrology, Environmental Science & Arid Land Agriculture, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. FAU - Farraj, Suha A AU - Farraj SA AD - Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. FAU - Hassan, Ahmed M AU - Hassan AM AD - Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. FAU - Al-Saeed, Muneera S AU - Al-Saeed MS AD - Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. FAU - Jamjoom, Ghazi A AU - Jamjoom GA AD - Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. FAU - Madani, Tariq A AU - Madani TA LA - eng SI - GENBANK/KJ740998 SI - GENBANK/KJ740999 SI - GENBANK/KJ741000 SI - GENBANK/KJ741001 SI - GENBANK/KJ741002 SI - GENBANK/KJ741003 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140722 PL - United States TA - mBio JT - mBio JID - 101519231 SB - IM MH - Animals MH - *Camelus MH - Coronavirus/*genetics MH - Genome, Viral/genetics MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*genetics/isolation & purification MH - Molecular Sequence Data PMC - PMC4120199 EDAT- 2014/07/24 06:00 MHDA- 2015/05/13 06:00 PMCR- 2014/07/22 CRDT- 2014/07/24 06:00 PHST- 2014/07/24 06:00 [entrez] PHST- 2014/07/24 06:00 [pubmed] PHST- 2015/05/13 06:00 [medline] PHST- 2014/07/22 00:00 [pmc-release] AID - mBio.01450-14 [pii] AID - mBio01450-14 [pii] AID - 10.1128/mBio.01450-14 [doi] PST - epublish SO - mBio. 2014 Jul 22;5(4):e01450-14. doi: 10.1128/mBio.01450-14. PMID- 28888653 OWN - NLM STAT- MEDLINE DCOM- 20180420 LR - 20200403 IS - 1873-2542 (Electronic) IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 208 DP - 2017 Sep TI - Porcine epidemic diarrhea virus does not replicate in porcine monocyte-derived dendritic cells, but activates the transcription of type I interferon and chemokine. PG - 77-81 LID - S0378-1135(17)30666-1 [pii] LID - 10.1016/j.vetmic.2017.07.014 [doi] AB - Porcine epidemic diarrhea virus (PEDV) belongs to the alphacoronavirus of the Coronaviridae. It is the major etiological agent of the recent outbreaks of piglet diarrhea and death in the US. Limited knowledge is currently available regarding the role of dendritic cells in PEDV infection. Here, we observed that PEDV did not replicate in monocyte-derived dendritic cells as evidenced by the decrease of viral gene transcript copies in infected cells by qRT-PCR and the absence of viral proteins by immunofluorescence staining as well as the absence of virus particles in infected cells by transmission electron microscopy. In addition, PEDV did not compromise cell viability at 48, 72, and 96h after infection at either a MOI of 2.5 or 5. Interestingly, an increased transcription of type I interferon including interferon-α and β was observed in infected cells compared to mock infected cells. Surprisingly, we did not detect any interferon-β in the supernatants of infected cells. A slight increase in interferon-α protein production in the supernatants of PEDV-infected cells was observed compared to mock infected cells. We also observed a markedly increased transcription of interferon inducible protein -10 (IP-10). Overall, PEDV does not replicate in porcine Mo-DC, but activates the transcription of type I interferon and chemokine IP-10. CI - Copyright © 2017 Elsevier B.V. All rights reserved. FAU - Wang, Xiuqing AU - Wang X AD - Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, United States, United States. Electronic address: xiuqing.wang@sdstate.edu. FAU - Ohnstad, Martha AU - Ohnstad M AD - Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, United States, United States. FAU - Nelsen, April AU - Nelsen A AD - Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, United States, United States. FAU - Nelson, Eric AU - Nelson E AD - Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, United States. LA - eng PT - Journal Article DEP - 20170717 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Chemokines) RN - 0 (Interferon Type I) SB - IM MH - Animals MH - Cell Survival MH - Chemokines/*metabolism MH - Chlorocebus aethiops MH - Dendritic Cells/*virology MH - Gene Expression Regulation/immunology MH - Interferon Type I/*metabolism MH - Porcine epidemic diarrhea virus/genetics/*physiology MH - *Swine MH - Vero Cells MH - Virus Replication/*physiology PMC - PMC7117325 OTO - NOTNLM OT - IP-10 OT - Mo-DC OT - PEDV OT - Type I interferon EDAT- 2017/09/11 06:00 MHDA- 2018/04/21 06:00 PMCR- 2017/07/17 CRDT- 2017/09/11 06:00 PHST- 2017/05/26 00:00 [received] PHST- 2017/07/10 00:00 [revised] PHST- 2017/07/14 00:00 [accepted] PHST- 2017/09/11 06:00 [entrez] PHST- 2017/09/11 06:00 [pubmed] PHST- 2018/04/21 06:00 [medline] PHST- 2017/07/17 00:00 [pmc-release] AID - S0378-1135(17)30666-1 [pii] AID - 10.1016/j.vetmic.2017.07.014 [doi] PST - ppublish SO - Vet Microbiol. 2017 Sep;208:77-81. doi: 10.1016/j.vetmic.2017.07.014. Epub 2017 Jul 17. PMID- 28500421 OWN - NLM STAT- MEDLINE DCOM- 20180416 LR - 20200324 IS - 1612-9210 (Electronic) IS - 1612-9202 (Print) IS - 1612-9202 (Linking) VI - 14 IP - 2 DP - 2017 Jun TI - Seasonal Fluctuations of Astrovirus, But Not Coronavirus Shedding in Bats Inhabiting Human-Modified Tropical Forests. PG - 272-284 LID - 10.1007/s10393-017-1245-x [doi] AB - Emerging infectious diseases (EIDs) are considered a major threat to global health. Most EIDs appear to result from increased contact between wildlife and humans, especially when humans encroach into formerly pristine habitats. Habitat deterioration may also negatively affect the physiology and health of wildlife species, which may eventually lead to a higher susceptibility to infectious agents and/or increased shedding of the pathogens causing EIDs. Bats are known to host viruses closely related to important EIDs. Here, we tested in a paleotropical forest with ongoing logging and fragmentation, whether habitat disturbance influences the occurrence of astro- and coronaviruses in eight bat species. In contrast to our hypothesis, anthropogenic habitat disturbance was not associated with corona- and astrovirus detection rates in fecal samples. However, we found that bats infected with either astro- or coronaviruses were likely to be coinfected with the respective other virus. Additionally, we identified two more risk factors influencing astrovirus shedding. First, the detection rate of astroviruses was higher at the beginning of the rainy compared to the dry season. Second, there was a trend that individuals with a poor body condition had a higher probability of shedding astroviruses in their feces. The identification of risk factors for increased viral shedding that may potentially result in increased interspecies transmission is important to prevent viral spillovers from bats to other animals, including humans. FAU - Seltmann, Anne AU - Seltmann A AD - Department of Evolutionary Ecology, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315, Berlin, Germany. Seltmann@izw-berlin.de. AD - Institute of Biology, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany. Seltmann@izw-berlin.de. FAU - Corman, Victor M AU - Corman VM AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. AD - German Centre for Infection Research, partner site Bonn-Cologne, Bonn, Germany. FAU - Rasche, Andrea AU - Rasche A AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. AD - German Centre for Infection Research, partner site Bonn-Cologne, Bonn, Germany. FAU - Drosten, Christian AU - Drosten C AD - Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. AD - German Centre for Infection Research, partner site Bonn-Cologne, Bonn, Germany. FAU - Czirják, Gábor Á AU - Czirják GÁ AD - Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315, Berlin, Germany. FAU - Bernard, Henry AU - Bernard H AD - Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia. FAU - Struebig, Matthew J AU - Struebig MJ AD - Durrell Institute of Conservation and Ecology (DICE), School of Anthropology and Conservation, University of Kent, Canterbury, Kent, CT2 7NR, UK. FAU - Voigt, Christian C AU - Voigt CC AD - Department of Evolutionary Ecology, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315, Berlin, Germany. AD - Institute of Biology, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany. LA - eng PT - Journal Article DEP - 20170512 PL - United States TA - Ecohealth JT - EcoHealth JID - 101222144 SB - IM MH - Animals MH - *Astroviridae MH - Chiroptera/*virology MH - *Coronavirus MH - Forests MH - Phylogeny MH - Seasons MH - *Virus Shedding PMC - PMC7087689 OTO - NOTNLM OT - Astroviruses OT - Bats OT - Coinfection OT - Coronaviruses OT - Habitat fragmentation OT - Human-modified landscapes COIS- The authors declare that they have no conflict of interest. EDAT- 2017/05/14 06:00 MHDA- 2018/04/17 06:00 PMCR- 2020/03/23 CRDT- 2017/05/14 06:00 PHST- 2016/10/31 00:00 [received] PHST- 2017/04/06 00:00 [accepted] PHST- 2017/04/06 00:00 [revised] PHST- 2017/05/14 06:00 [pubmed] PHST- 2018/04/17 06:00 [medline] PHST- 2017/05/14 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s10393-017-1245-x [pii] AID - 1245 [pii] AID - 10.1007/s10393-017-1245-x [doi] PST - ppublish SO - Ecohealth. 2017 Jun;14(2):272-284. doi: 10.1007/s10393-017-1245-x. Epub 2017 May 12. PMID- 25972557 OWN - NLM STAT- MEDLINE DCOM- 20151009 LR - 20181113 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 15 DP - 2015 Aug TI - Transcriptome Profiling of the Virus-Induced Innate Immune Response in Pteropus vampyrus and Its Attenuation by Nipah Virus Interferon Antagonist Functions. PG - 7550-66 LID - 10.1128/JVI.00302-15 [doi] AB - Bats are important reservoirs for several viruses, many of which cause lethal infections in humans but have reduced pathogenicity in bats. As the innate immune response is critical for controlling viruses, the nature of this response in bats and how it may differ from that in other mammals are of great interest. Using next-generation transcriptome sequencing (mRNA-seq), we profiled the transcriptional response of Pteropus vampyrus bat kidney (PVK) cells to Newcastle disease virus (NDV), an avian paramyxovirus known to elicit a strong innate immune response in mammalian cells. The Pteropus genus is a known reservoir of Nipah virus (NiV) and Hendra virus (HeV). Analysis of the 200 to 300 regulated genes showed that genes for interferon (IFN) and antiviral pathways are highly upregulated in NDV-infected PVK cells, including genes for beta IFN, RIG-I, MDA5, ISG15, and IRF1. NDV-infected cells also upregulated several genes not previously characterized to be antiviral, such as RND1, SERTAD1, CHAC1, and MORC3. In fact, we show that MORC3 is induced by both IFN and NDV infection in PVK cells but is not induced by either stimulus in human A549 cells. In contrast to NDV infection, HeV and NiV infection of PVK cells failed to induce these innate immune response genes. Likewise, an attenuated response was observed in PVK cells infected with recombinant NDVs expressing the NiV IFN antagonist proteins V and W. This study provides the first global profile of a robust virus-induced innate immune response in bats and indicates that henipavirus IFN antagonist mechanisms are likely active in bat cells. IMPORTANCE: Bats are the reservoir host for many highly pathogenic human viruses, including henipaviruses, lyssaviruses, severe acute respiratory syndrome coronavirus, and filoviruses, and many other viruses have also been isolated from bats. Viral infections are reportedly asymptomatic or heavily attenuated in bat populations. Despite their ecological importance to viral maintenance, research into their immune system and mechanisms for viral control has only recently begun. Nipah virus and Hendra virus are two paramyxoviruses associated with high mortality rates in humans and whose reservoir is the Pteropus genus of bats. Greater knowledge of the innate immune response of P. vampyrus bats to viral infection may elucidate how bats serve as a reservoir for so many viruses. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Glennon, Nicole B AU - Glennon NB AD - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA. FAU - Jabado, Omar AU - Jabado O AD - Institute for Genomics and Multiscale Biology, Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA. FAU - Lo, Michael K AU - Lo MK AD - Centers for Disease Control and Prevention, Viral Special Pathogens Branch, Atlanta, Georgia, USA. FAU - Shaw, Megan L AU - Shaw ML AD - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA megan.shaw@mssm.edu. LA - eng GR - T32 AI007647/AI/NIAID NIH HHS/United States GR - R21 AI102169/AI/NIAID NIH HHS/United States GR - R01 AI101308/AI/NIAID NIH HHS/United States GR - HHSN272200900032C/AI/NIAID NIH HHS/United States GR - T32AI007647/AI/NIAID NIH HHS/United States GR - HHSN272200900032C/PHS HHS/United States GR - R21AI102169/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20150513 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 9008-11-1 (Interferons) SB - IM MH - Animals MH - Chiroptera/genetics/*immunology/virology MH - Disease Reservoirs/*virology MH - *Gene Expression Profiling MH - Hendra Virus/genetics/immunology/physiology MH - Henipavirus Infections/genetics/*immunology/virology MH - Humans MH - Immune Evasion MH - *Immunity, Innate MH - Interferons/genetics/*immunology MH - Newcastle disease virus/genetics/immunology/physiology MH - Nipah Virus/genetics/*immunology/physiology PMC - PMC4505658 EDAT- 2015/05/15 06:00 MHDA- 2015/10/10 06:00 PMCR- 2016/02/01 CRDT- 2015/05/15 06:00 PHST- 2015/02/04 00:00 [received] PHST- 2015/05/02 00:00 [accepted] PHST- 2015/05/15 06:00 [entrez] PHST- 2015/05/15 06:00 [pubmed] PHST- 2015/10/10 06:00 [medline] PHST- 2016/02/01 00:00 [pmc-release] AID - JVI.00302-15 [pii] AID - 00302-15 [pii] AID - 10.1128/JVI.00302-15 [doi] PST - ppublish SO - J Virol. 2015 Aug;89(15):7550-66. doi: 10.1128/JVI.00302-15. Epub 2015 May 13. PMID- 29190287 OWN - NLM STAT- MEDLINE DCOM- 20171214 LR - 20221207 IS - 1553-7374 (Electronic) IS - 1553-7366 (Print) IS - 1553-7366 (Linking) VI - 13 IP - 11 DP - 2017 Nov TI - Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus. PG - e1006698 LID - 10.1371/journal.ppat.1006698 [doi] LID - e1006698 AB - A large number of SARS-related coronaviruses (SARSr-CoV) have been detected in horseshoe bats since 2005 in different areas of China. However, these bat SARSr-CoVs show sequence differences from SARS coronavirus (SARS-CoV) in different genes (S, ORF8, ORF3, etc) and are considered unlikely to represent the direct progenitor of SARS-CoV. Herein, we report the findings of our 5-year surveillance of SARSr-CoVs in a cave inhabited by multiple species of horseshoe bats in Yunnan Province, China. The full-length genomes of 11 newly discovered SARSr-CoV strains, together with our previous findings, reveals that the SARSr-CoVs circulating in this single location are highly diverse in the S gene, ORF3 and ORF8. Importantly, strains with high genetic similarity to SARS-CoV in the hypervariable N-terminal domain (NTD) and receptor-binding domain (RBD) of the S1 gene, the ORF3 and ORF8 region, respectively, were all discovered in this cave. In addition, we report the first discovery of bat SARSr-CoVs highly similar to human SARS-CoV in ORF3b and in the split ORF8a and 8b. Moreover, SARSr-CoV strains from this cave were more closely related to SARS-CoV in the non-structural protein genes ORF1a and 1b compared with those detected elsewhere. Recombination analysis shows evidence of frequent recombination events within the S gene and around the ORF8 between these SARSr-CoVs. We hypothesize that the direct progenitor of SARS-CoV may have originated after sequential recombination events between the precursors of these SARSr-CoVs. Cell entry studies demonstrated that three newly identified SARSr-CoVs with different S protein sequences are all able to use human ACE2 as the receptor, further exhibiting the close relationship between strains in this cave and SARS-CoV. This work provides new insights into the origin and evolution of SARS-CoV and highlights the necessity of preparedness for future emergence of SARS-like diseases. FAU - Hu, Ben AU - Hu B AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zeng, Lei-Ping AU - Zeng LP AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Yang, Xing-Lou AU - Yang XL AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Ge, Xing-Yi AU - Ge XY AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zhang, Wei AU - Zhang W AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Li, Bei AU - Li B AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Xie, Jia-Zheng AU - Xie JZ AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Shen, Xu-Rui AU - Shen XR AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zhang, Yun-Zhi AU - Zhang YZ AD - Yunnan Institute of Endemic Diseases Control and Prevention, Dali, China. AD - Dali University, Dali, China. FAU - Wang, Ning AU - Wang N AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Luo, Dong-Sheng AU - Luo DS AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zheng, Xiao-Shuang AU - Zheng XS AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Wang, Mei-Niang AU - Wang MN AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Daszak, Peter AU - Daszak P AUID- ORCID: 0000-0002-2046-5695 AD - EcoHealth Alliance, New York, New York, United States of America. FAU - Wang, Lin-Fa AU - Wang LF AD - Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore. FAU - Cui, Jie AU - Cui J AUID- ORCID: 0000-0001-8176-9951 AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Shi, Zheng-Li AU - Shi ZL AUID- ORCID: 0000-0001-8089-163X AD - CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases of Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. LA - eng GR - R01 AI110964/AI/NIAID NIH HHS/United States PT - Journal Article DEP - 20171130 PL - United States TA - PLoS Pathog JT - PLoS pathogens JID - 101238921 SB - IM CIN - Nature. 2018 Jan 18;553(7688):281. doi: 10.1038/d41586-018-00603-7. PMID: 29345663 MH - Amino Acid Sequence/genetics MH - Animals MH - Chiroptera/*virology MH - Coronavirus Infections/virology MH - Evolution, Molecular MH - *Gene Pool MH - Genome, Viral/*genetics MH - Humans MH - Recombination, Genetic/genetics MH - Severe acute respiratory syndrome-related coronavirus/*genetics MH - Severe Acute Respiratory Syndrome/*virology PMC - PMC5708621 COIS- The authors have declared that no competing interests exist. EDAT- 2017/12/01 06:00 MHDA- 2017/12/15 06:00 PMCR- 2017/11/30 CRDT- 2017/12/01 06:00 PHST- 2017/02/10 00:00 [received] PHST- 2017/10/17 00:00 [accepted] PHST- 2017/12/01 06:00 [entrez] PHST- 2017/12/01 06:00 [pubmed] PHST- 2017/12/15 06:00 [medline] PHST- 2017/11/30 00:00 [pmc-release] AID - PPATHOGENS-D-17-00265 [pii] AID - 10.1371/journal.ppat.1006698 [doi] PST - epublish SO - PLoS Pathog. 2017 Nov 30;13(11):e1006698. doi: 10.1371/journal.ppat.1006698. eCollection 2017 Nov. PMID- 26085157 OWN - NLM STAT- MEDLINE DCOM- 20160429 LR - 20191210 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 89 IP - 17 DP - 2015 Sep TI - Identification of the Receptor-Binding Domain of the Spike Glycoprotein of Human Betacoronavirus HKU1. PG - 8816-27 LID - 10.1128/JVI.03737-14 [doi] AB - Coronavirus spike (S) glycoproteins mediate receptor binding, membrane fusion, and virus entry and determine host range. Murine betacoronavirus (β-CoV) in group A uses the N-terminal domain (NTD) of S protein to bind to its receptor, whereas the β-CoVs severe acute respiratory syndrome CoV in group B and Middle East respiratory syndrome CoV in group C and several α-CoVs use the downstream C domain in their S proteins to recognize their receptor proteins. To identify the receptor-binding domain in the spike of human β-CoV HKU1 in group A, we generated and mapped a panel of monoclonal antibodies (MAbs) to the ectodomain of HKU1 spike protein. They did not cross-react with S proteins of any other CoV tested. Most of the HKU1 spike MAbs recognized epitopes in the C domain between amino acids 535 and 673, indicating that this region is immunodominant. Two of the MAbs blocked HKU1 virus infection of primary human tracheal-bronchial epithelial (HTBE) cells. Preincubation of HTBE cells with a truncated HKU1 S protein that includes the C domain blocked infection with HKU1 virus, but preincubation of cells with truncated S protein containing only the NTD did not block infection. These data suggest that the receptor-binding domain (RBD) of HKU1 spike protein is located in the C domain, where the spike proteins of α-CoVs and β-CoVs in groups B and C bind to their specific receptor proteins. Thus, two β-CoVs in group A, HKU1 and murine CoV, have evolved to use different regions of their spike glycoproteins to recognize their respective receptor proteins. IMPORTANCE: Mouse hepatitis virus, a β-CoV in group A, uses the galectin-like NTD in its spike protein to bind its receptor protein, while HCoV-OC43, another β-CoV in group A, uses the NTD to bind to its sialic-acid containing receptor. In marked contrast, the NTD of the spike glycoprotein of human respiratory β-CoV HKU1, which is also in group A, does not bind sugar. In this study, we showed that for the spike protein of HKU1, the purified C domain, downstream of the NTD, could block HKU1 virus infection of human respiratory epithelial cells, and that several monoclonal antibodies that mapped to the C domain neutralized virus infectivity. Thus, the receptor-binding domain of HKU1 spike glycoprotein is located in the C domain. Surprisingly, two β-CoVs in group A, mouse hepatitis virus and HKU1, have evolved to use different regions of their spike glycoproteins to recognize their respective receptors. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Qian, Zhaohui AU - Qian Z AD - MOH Key Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Science, Beijing, China Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, USA zqian2013@sina.com Samuel.dominguez@ucdenver.edu. FAU - Ou, Xiuyuan AU - Ou X AD - MOH Key Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Science, Beijing, China. FAU - Góes, Luiz Gustavo Bentim AU - Góes LG AD - Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, USA. FAU - Osborne, Christina AU - Osborne C AD - Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, USA. FAU - Castano, Anna AU - Castano A AD - Department of Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA. FAU - Holmes, Kathryn V AU - Holmes KV AD - Department of Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA. FAU - Dominguez, Samuel R AU - Dominguez SR AD - Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, USA zqian2013@sina.com Samuel.dominguez@ucdenver.edu. LA - eng GR - P30 CA046934/CA/NCI NIH HHS/United States GR - 5K08-A1073525/PHS HHS/United States GR - P30CA046934/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20150617 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Antibodies, Monoclonal) RN - 0 (Receptors, Virus) RN - 0 (Spike Glycoprotein, Coronavirus) SB - IM MH - Amino Acid Sequence MH - Animals MH - Antibodies, Monoclonal/immunology MH - Cell Line, Transformed MH - Chlorocebus aethiops MH - Coronavirus/genetics/immunology/*metabolism MH - Coronavirus Infections/*virology MH - Dogs MH - Epithelial Cells/virology MH - HEK293 Cells MH - Humans MH - Madin Darby Canine Kidney Cells MH - Molecular Sequence Data MH - Murine hepatitis virus/genetics/metabolism MH - Protein Structure, Tertiary MH - Receptors, Virus/*genetics MH - Respiratory Mucosa/cytology/virology MH - Sequence Alignment MH - Spike Glycoprotein, Coronavirus/*genetics/immunology MH - Vero Cells MH - Viral Tropism/*genetics MH - Virus Internalization PMC - PMC4524053 EDAT- 2015/06/19 06:00 MHDA- 2016/04/30 06:00 PMCR- 2015/12/17 CRDT- 2015/06/19 06:00 PHST- 2014/12/31 00:00 [received] PHST- 2015/06/04 00:00 [accepted] PHST- 2015/06/19 06:00 [entrez] PHST- 2015/06/19 06:00 [pubmed] PHST- 2016/04/30 06:00 [medline] PHST- 2015/12/17 00:00 [pmc-release] AID - JVI.03737-14 [pii] AID - 03737-14 [pii] AID - 10.1128/JVI.03737-14 [doi] PST - ppublish SO - J Virol. 2015 Sep;89(17):8816-27. doi: 10.1128/JVI.03737-14. Epub 2015 Jun 17. PMID- 25487801 OWN - NLM STAT- MEDLINE DCOM- 20151019 LR - 20240613 IS - 1098-6596 (Electronic) IS - 0066-4804 (Print) IS - 0066-4804 (Linking) VI - 59 IP - 2 DP - 2015 Feb TI - Antiviral potential of ERK/MAPK and PI3K/AKT/mTOR signaling modulation for Middle East respiratory syndrome coronavirus infection as identified by temporal kinome analysis. PG - 1088-99 LID - 10.1128/AAC.03659-14 [doi] AB - Middle East respiratory syndrome coronavirus (MERS-CoV) is a lineage C betacoronavirus, and infections with this virus can result in acute respiratory syndrome with renal failure. Globally, MERS-CoV has been responsible for 877 laboratory-confirmed infections, including 317 deaths, since September 2012. As there is a paucity of information regarding the molecular pathogenesis associated with this virus or the identities of novel antiviral drug targets, we performed temporal kinome analysis on human hepatocytes infected with the Erasmus isolate of MERS-CoV with peptide kinome arrays. bioinformatics analysis of our kinome data, including pathway overrepresentation analysis (ORA) and functional network analysis, suggested that extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) and phosphoinositol 3-kinase (PI3K)/serine-threonine kinase (AKT)/mammalian target of rapamycin (mTOR) signaling responses were specifically modulated in response to MERS-CoV infection in vitro throughout the course of infection. The overrepresentation of specific intermediates within these pathways determined by pathway and functional network analysis of our kinome data correlated with similar patterns of phosphorylation determined through Western blot array analysis. In addition, analysis of the effects of specific kinase inhibitors on MERS-CoV infection in tissue culture models confirmed these cellular response observations. Further, we have demonstrated that a subset of licensed kinase inhibitors targeting the ERK/MAPK and PI3K/AKT/mTOR pathways significantly inhibited MERS-CoV replication in vitro whether they were added before or after viral infection. Taken together, our data suggest that ERK/MAPK and PI3K/AKT/mTOR signaling responses play important roles in MERS-CoV infection and may represent novel drug targets for therapeutic intervention strategies. CI - Copyright © 2015, American Society for Microbiology. All Rights Reserved. FAU - Kindrachuk, Jason AU - Kindrachuk J AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA kindrachuk.kenneth@nih.gov. FAU - Ork, Britini AU - Ork B AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Hart, Brit J AU - Hart BJ AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Mazur, Steven AU - Mazur S AUID- ORCID: 0000-0002-9349-338X AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Holbrook, Michael R AU - Holbrook MR AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Frieman, Matthew B AU - Frieman MB AD - Department of Microbiology and Immunology, University of Maryland at Baltimore, Baltimore, Maryland, USA. FAU - Traynor, Dawn AU - Traynor D AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Johnson, Reed F AU - Johnson RF AD - Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Dyall, Julie AU - Dyall J AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Kuhn, Jens H AU - Kuhn JH AUID- ORCID: 0000-0002-7800-6045 AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Olinger, Gene G AU - Olinger GG AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Hensley, Lisa E AU - Hensley LE AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. FAU - Jahrling, Peter B AU - Jahrling PB AUID- ORCID: 0000-0002-9775-3724 AD - Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. LA - eng GR - HHSN272200700016I/AI/NIAID NIH HHS/United States GR - R01 AI095569/AI/NIAID NIH HHS/United States GR - ImNIH/Intramural NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, N.I.H., Intramural DEP - 20141208 PL - United States TA - Antimicrob Agents Chemother JT - Antimicrobial agents and chemotherapy JID - 0315061 RN - EC 2.7.1.1 (MTOR protein, human) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) SB - IM MH - Blotting, Western MH - Cell Line MH - Computational Biology MH - Coronavirus Infections/*metabolism/virology MH - Enzyme-Linked Immunosorbent Assay MH - Extracellular Signal-Regulated MAP Kinases/metabolism MH - Hepatocytes/*metabolism/*virology MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*physiology MH - Phosphatidylinositol 3-Kinases/*metabolism MH - Phosphorylation MH - Signal Transduction/physiology MH - TOR Serine-Threonine Kinases/*metabolism PMC - PMC4335870 EDAT- 2014/12/10 06:00 MHDA- 2015/10/20 06:00 PMCR- 2015/08/01 CRDT- 2014/12/10 06:00 PHST- 2014/12/10 06:00 [entrez] PHST- 2014/12/10 06:00 [pubmed] PHST- 2015/10/20 06:00 [medline] PHST- 2015/08/01 00:00 [pmc-release] AID - AAC.03659-14 [pii] AID - 03659-14 [pii] AID - 10.1128/AAC.03659-14 [doi] PST - ppublish SO - Antimicrob Agents Chemother. 2015 Feb;59(2):1088-99. doi: 10.1128/AAC.03659-14. Epub 2014 Dec 8. PMID- 30384140 OWN - NLM STAT- MEDLINE DCOM- 20190131 LR - 20231113 IS - 1532-2661 (Electronic) IS - 0034-5288 (Print) IS - 0034-5288 (Linking) VI - 121 DP - 2018 Dec TI - Development and validation of a monoclonal antibody-based competitive ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV). PG - 106-110 LID - S0034-5288(18)30916-0 [pii] LID - 10.1016/j.rvsc.2018.10.011 [doi] AB - Porcine epidemic diarrhoea virus (PEDV), belongs to the genus Alphacoronavirus in the family Coronaviridae and causes severe diarrhoea, vomiting, dehydration and high mortality in seronegative newborn piglets. Thus, a precise and rapid diagnosis of PEDV infection is important for the application of control measures to limit viral dissemination. In this investigation, a monoclonal antibodies (MAbs)-based competitive enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against PEDV was developed and validated. The diagnostic performance of the test was evaluated by receiver operating characteristic (ROC) analysis using a panel of 829 known sera collected from different commercial pig farms, with or without a history of PED presence and from experimentally infected pigs. The competitive ELISA showed excellent diagnostic performance and discriminatory power with high sensitivity (Se) and specificity (Sp) values (Se = 96.5%, 95% IC 94.1-98.1; Sp = 98.2%, 95% IC 96.3-99.3). Moreover, this competitive ELISA method has other properties, such as high feasibility of testing sera without pre-treatment and automatic and instrument-mediated revealing, that makes it appropriate for large-scale screenings of affected pig farms in endemic regions or for monitoring plans in PEDV-free areas. CI - Copyright © 2018 Elsevier Ltd. All rights reserved. FAU - Sozzi, Enrica AU - Sozzi E AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. Electronic address: enrica.sozzi@izsler.it. FAU - Moreno, Ana AU - Moreno A AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Lelli, Davide AU - Lelli D AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Perulli, Simona AU - Perulli S AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Prosperi, Alice AU - Prosperi A AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Brocchi, Emiliana AU - Brocchi E AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Capucci, Lorenzo AU - Capucci L AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Papetti, Alice AU - Papetti A AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Giacomini, Enrico AU - Giacomini E AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Alborali, Giovanni Loris AU - Alborali GL AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. FAU - Lavazza, Antonio AU - Lavazza A AD - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", Via Bianchi, 9, 25124 Brescia, Italy. LA - eng PT - Journal Article PT - Validation Study DEP - 20181022 PL - England TA - Res Vet Sci JT - Research in veterinary science JID - 0401300 RN - 0 (Antibodies, Monoclonal) RN - 0 (Antibodies, Viral) SB - IM MH - Animals MH - Antibodies, Monoclonal/blood MH - Antibodies, Viral/blood MH - Coronavirus Infections/diagnosis/*veterinary/virology MH - Enzyme-Linked Immunosorbent Assay/methods/*veterinary MH - Porcine epidemic diarrhea virus/*isolation & purification MH - Sensitivity and Specificity MH - Swine MH - Swine Diseases/*diagnosis/virology PMC - PMC7111896 OTO - NOTNLM OT - Competitive ELISA OT - Monoclonal antibodies OT - PEDV EDAT- 2018/11/02 06:00 MHDA- 2019/02/01 06:00 PMCR- 2018/10/22 CRDT- 2018/11/02 06:00 PHST- 2018/06/05 00:00 [received] PHST- 2018/10/18 00:00 [revised] PHST- 2018/10/21 00:00 [accepted] PHST- 2018/11/02 06:00 [pubmed] PHST- 2019/02/01 06:00 [medline] PHST- 2018/11/02 06:00 [entrez] PHST- 2018/10/22 00:00 [pmc-release] AID - S0034-5288(18)30916-0 [pii] AID - 10.1016/j.rvsc.2018.10.011 [doi] PST - ppublish SO - Res Vet Sci. 2018 Dec;121:106-110. doi: 10.1016/j.rvsc.2018.10.011. Epub 2018 Oct 22. PMID- 28747502 OWN - NLM STAT- MEDLINE DCOM- 20170926 LR - 20191210 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 91 IP - 19 DP - 2017 Oct 1 TI - Permissivity of Dipeptidyl Peptidase 4 Orthologs to Middle East Respiratory Syndrome Coronavirus Is Governed by Glycosylation and Other Complex Determinants. LID - 10.1128/JVI.00534-17 [doi] LID - e00534-17 AB - Middle East respiratory syndrome coronavirus (MERS-CoV) utilizes dipeptidyl peptidase 4 (DPP4) as an entry receptor. While bat, camel, and human DPP4 support MERS-CoV infection, several DPP4 orthologs, including mouse, ferret, hamster, and guinea pig DPP4, do not. Previous work revealed that glycosylation of mouse DPP4 plays a role in blocking MERS-CoV infection. Here, we tested whether glycosylation also acts as a determinant of permissivity for ferret, hamster, and guinea pig DPP4. We found that, while glycosylation plays an important role in these orthologs, additional sequence and structural determinants impact their ability to act as functional receptors for MERS-CoV. These results provide insight into DPP4 species-specific differences impacting MERS-CoV host range and better inform our understanding of virus-receptor interactions associated with disease emergence and host susceptibility.IMPORTANCE MERS-CoV is a recently emerged zoonotic virus that is still circulating in the human population with an ∼35% mortality rate. With no available vaccines or therapeutics, the study of MERS-CoV pathogenesis is crucial for its control and prevention. However, in vivo studies are limited because MERS-CoV cannot infect wild-type mice due to incompatibilities between the virus spike and the mouse host cell receptor, mouse DPP4 (mDPP4). Specifically, mDPP4 has a nonconserved glycosylation site that acts as a barrier to MERS-CoV infection. Thus, one mouse model strategy has been to modify the mouse genome to remove this glycosylation site. Here, we investigated whether glycosylation acts as a barrier to infection for other nonpermissive small-animal species, namely, ferret, guinea pig, and hamster. Understanding the virus-receptor interactions for these DPP4 orthologs will help in the development of additional animal models while also revealing species-specific differences impacting MERS-CoV host range. CI - Copyright © 2017 American Society for Microbiology. FAU - Peck, Kayla M AU - Peck KM AUID- ORCID: 0000-0001-7745-6995 AD - Department of Biology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Scobey, Trevor AU - Scobey T AD - Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Swanstrom, Jesica AU - Swanstrom J AD - Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Jensen, Kara L AU - Jensen KL AD - Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Burch, Christina L AU - Burch CL AD - Department of Biology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Baric, Ralph S AU - Baric RS AD - Department of Epidemiology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA rbaric@email.unc.edu mark_heisem@med.unc.edu. AD - Department of Microbiology and Immunology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA. FAU - Heise, Mark T AU - Heise MT AD - Department of Genetics, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA rbaric@email.unc.edu mark_heisem@med.unc.edu. AD - Department of Microbiology and Immunology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA. LA - eng GR - R01 AI110700/AI/NIAID NIH HHS/United States GR - U19 AI109761/AI/NIAID NIH HHS/United States PT - Journal Article DEP - 20170912 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Receptors, Virus) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Amino Acid Sequence/genetics MH - Animals MH - Cell Line MH - Chlorocebus aethiops MH - Coronavirus Infections/genetics/*pathology/virology MH - Cricetinae MH - Dipeptidyl Peptidase 4/genetics/*metabolism MH - Ferrets MH - Glycosylation MH - Guinea Pigs MH - HEK293 Cells MH - Host Specificity/*physiology MH - Humans MH - Middle East Respiratory Syndrome Coronavirus/*metabolism MH - Receptors, Virus/genetics/*metabolism MH - Sequence Alignment MH - Sequence Homology, Amino Acid MH - Vero Cells MH - *Virus Attachment PMC - PMC5599747 OTO - NOTNLM OT - DPP4 OT - MERS-coronavirus OT - animal models OT - glycosylation OT - host range OT - host range expansion OT - orthologs EDAT- 2017/07/28 06:00 MHDA- 2017/09/28 06:00 PMCR- 2018/03/12 CRDT- 2017/07/28 06:00 PHST- 2017/03/30 00:00 [received] PHST- 2017/07/13 00:00 [accepted] PHST- 2017/07/28 06:00 [pubmed] PHST- 2017/09/28 06:00 [medline] PHST- 2017/07/28 06:00 [entrez] PHST- 2018/03/12 00:00 [pmc-release] AID - JVI.00534-17 [pii] AID - 00534-17 [pii] AID - 10.1128/JVI.00534-17 [doi] PST - epublish SO - J Virol. 2017 Sep 12;91(19):e00534-17. doi: 10.1128/JVI.00534-17. Print 2017 Oct 1. PMID- 30652967 OWN - NLM STAT- MEDLINE DCOM- 20191023 LR - 20191023 IS - 1465-2099 (Electronic) IS - 0022-1317 (Linking) VI - 100 IP - 2 DP - 2019 Feb TI - Two critical N-terminal epitopes of the nucleocapsid protein contribute to the cross-reactivity between porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus. PG - 206-216 LID - 10.1099/jgv.0.001216 [doi] AB - Both porcine epidemic diarrhoea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV), which cause high mortality in piglets and produce similar clinical symptoms and histopathological morphology, belong to the genus Alphacoronavirus. Serological diagnosis plays an important role in distinguishing pathogen species. Together with the spike (S) protein, the nucleocapsid (N) protein is one of the immunodominant regions among coronaviruses. In this study, two-way antigenic cross-reactivity between the N proteins of PEDV and TGEV was observed by indirect immunofluorescence assay (IFA) and Western blot analysis. Furthermore, the PEDV N protein harbouring truncations of amino acids (aa) 1 to 170 or aa 125 to 301 was demonstrated to cross-react with the anti-TGEV N polyclonal antibody (PAb), whereas the truncation-expressing aa 302 to 401 resulted in a specific reaction with the anti-PEDV N PAb but not with the anti-TGEV N PAb. Mutants of the PEDV N protein were generated based on sequence alignment and structural analysis; we then confirmed that the N-terminal residues 58-RWRMRRGERIE-68 and 78-LGTGPHAD-85 contributed to the cross-reactivity. All the results provide vital clues for the development of precise diagnostic assays for porcine coronaviruses. FAU - Xie, Wenting AU - Xie W AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Ao, Chaojie AU - Ao C AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Yang, Yilin AU - Yang Y AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Liu, Yinan AU - Liu Y AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Liang, Rui AU - Liang R AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Zeng, Zhe AU - Zeng Z AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Ye, Gang AU - Ye G AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Xiao, Shaobo AU - Xiao S AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Fu, Zhen F AU - Fu ZF AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 4​Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. FAU - Dong, Wanyu AU - Dong W AD - 5​National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. AD - 3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. FAU - Peng, Guiqing AU - Peng G AD - 3​The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 2​College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China. AD - 1​State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, PR China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190117 PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (Antibodies, Viral) RN - 0 (Epitopes) RN - 0 (Nucleocapsid Proteins) SB - IM MH - Animals MH - Antibodies, Viral/*immunology MH - Blotting, Western MH - *Cross Reactions MH - Epitope Mapping MH - Epitopes/*immunology MH - Fluorescent Antibody Technique, Indirect MH - Nucleocapsid Proteins/*immunology MH - Porcine epidemic diarrhea virus/*immunology MH - Swine MH - Transmissible gastroenteritis virus/*immunology OTO - NOTNLM OT - N-terminal epitopes OT - PEDV OT - TGEV OT - cross-reactivity OT - nucleocapsid protein EDAT- 2019/01/18 06:00 MHDA- 2019/10/24 06:00 CRDT- 2019/01/18 06:00 PHST- 2019/01/18 06:00 [pubmed] PHST- 2019/10/24 06:00 [medline] PHST- 2019/01/18 06:00 [entrez] AID - 10.1099/jgv.0.001216 [doi] PST - ppublish SO - J Gen Virol. 2019 Feb;100(2):206-216. doi: 10.1099/jgv.0.001216. Epub 2019 Jan 17. PMID- 28374120 OWN - NLM STAT- MEDLINE DCOM- 20170802 LR - 20200325 IS - 1432-8798 (Electronic) IS - 0304-8608 (Print) IS - 0304-8608 (Linking) VI - 162 IP - 8 DP - 2017 Aug TI - Calves are susceptible to infection with the newly emerged porcine deltacoronavirus, but not with the swine enteric alphacoronavirus, porcine epidemic diarrhea virus. PG - 2357-2362 LID - 10.1007/s00705-017-3351-z [doi] AB - Fecal virus shedding, seroconversion and histopathology were evaluated in 3-7-year-old gnotobiotic calves orally inoculated with porcine deltacoronavirus (PDCoV) (9.0-9.6 log(10) genomic equivalents [GE] of OH-FD22-P5; n=4) or porcine epidemic diarrhea virus (PEDV) (10.2-12.5 log(10) GE of PC21A; n=3). In PDCoV-inoculated calves, an acute but persisting fecal viral RNA shedding and PDCoV-specific serum IgG antibody responses were observed, but without lesions or clinical disease. However, no fecal shedding, seroconversion, histological lesions, and clinical disease were detected in PEDV-inoculated calves. Our data indicate that calves are susceptible to infection by the newly emerged PDCoV, but not by the swine coronavirus, PEDV. FAU - Jung, Kwonil AU - Jung K AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., Wooster, OH, 44691, USA. jung.221@osu.edu. FAU - Hu, Hui AU - Hu H AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., Wooster, OH, 44691, USA. AD - College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China. FAU - Saif, Linda J AU - Saif LJ AD - Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., Wooster, OH, 44691, USA. saif.2@osu.edu. LA - eng PT - Journal Article DEP - 20170403 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 RN - 0 (Antibodies, Viral) RN - 0 (Immunoglobulin G) RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Antibodies, Viral/blood MH - Cattle/*virology MH - Cattle Diseases/*diagnosis/virology MH - Coronavirus/*classification/genetics MH - Coronavirus Infections/diagnosis/*veterinary/virology MH - Diarrhea/veterinary/virology MH - Feces/virology MH - Fluorescent Antibody Technique, Indirect MH - Immunoglobulin G/blood MH - RNA, Viral/analysis MH - Swine MH - Virus Shedding PMC - PMC7086908 COIS- Neither of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper. All authors have seen and approved the manuscript. EDAT- 2017/04/05 06:00 MHDA- 2017/08/03 06:00 PMCR- 2020/03/23 CRDT- 2017/04/05 06:00 PHST- 2016/12/22 00:00 [received] PHST- 2017/02/18 00:00 [accepted] PHST- 2017/04/05 06:00 [pubmed] PHST- 2017/08/03 06:00 [medline] PHST- 2017/04/05 06:00 [entrez] PHST- 2020/03/23 00:00 [pmc-release] AID - 10.1007/s00705-017-3351-z [pii] AID - 3351 [pii] AID - 10.1007/s00705-017-3351-z [doi] PST - ppublish SO - Arch Virol. 2017 Aug;162(8):2357-2362. doi: 10.1007/s00705-017-3351-z. Epub 2017 Apr 3. PMID- 27170748 OWN - NLM STAT- MEDLINE DCOM- 20170518 LR - 20221207 IS - 1098-5514 (Electronic) IS - 0022-538X (Print) IS - 0022-538X (Linking) VI - 90 IP - 14 DP - 2016 Jul 15 TI - Bat Severe Acute Respiratory Syndrome-Like Coronavirus WIV1 Encodes an Extra Accessory Protein, ORFX, Involved in Modulation of the Host Immune Response. PG - 6573-6582 LID - 10.1128/JVI.03079-15 [doi] AB - Bats harbor severe acute respiratory syndrome (SARS)-like coronaviruses (SL-CoVs) from which the causative agent of the 2002-2003 SARS pandemic is thought to have originated. However, despite the fact that a large number of genetically diverse SL-CoV sequences have been detected in bats, only two strains (named WIV1 and WIV16) have been successfully cultured in vitro These two strains differ from SARS-CoV only in containing an extra open reading frame (ORF) (named ORFX), between ORF6 and ORF7, which has no homology to any known protein sequences. In this study, we constructed a full-length cDNA clone of SL-CoV WIV1 (rWIV1), an ORFX deletion mutant (rWIV1-ΔX), and a green fluorescent protein (GFP)-expressing mutant (rWIV1-GFP-ΔX). Northern blotting and fluorescence microscopy indicate that ORFX was expressed during WIV1 infection. A virus infection assay showed that rWIV1-ΔX replicated as efficiently as rWIV1 in Vero E6, Calu-3, and HeLa-hACE2 cells. Further study showed that ORFX could inhibit interferon production and activate NF-κB. Our results demonstrate for the first time that the unique ORFX in the WIV1 strain is a functional gene involving modulation of the host immune response but is not essential for in vitro viral replication. IMPORTANCE: Bats harbor genetically diverse SARS-like coronaviruses (SL-CoVs), and some of them have the potential for interspecies transmission. A unique open reading frame (ORFX) was identified in the genomes of two recently isolated bat SL-CoV strains (WIV1 and -16). It will therefore be critical to clarify whether and how this protein contributes to virulence during viral infection. Here we revealed that the unique ORFX is a functional gene that is involved in the modulation of the host immune response but is not essential for in vitro viral replication. Our results provide important information for further exploration of the ORFX function in the future. Moreover, the reverse genetics system we constructed will be helpful for study of the pathogenesis of this group of viruses and to develop therapeutics for future control of emerging SARS-like infections. CI - Copyright © 2016, American Society for Microbiology. All Rights Reserved. FAU - Zeng, Lei-Ping AU - Zeng LP AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Gao, Yu-Tao AU - Gao YT AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Ge, Xing-Yi AU - Ge XY AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Zhang, Qian AU - Zhang Q AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Peng, Cheng AU - Peng C AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Yang, Xing-Lou AU - Yang XL AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Tan, Bing AU - Tan B AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Chen, Jing AU - Chen J AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China. FAU - Chmura, Aleksei A AU - Chmura AA AUID- ORCID: 0000-0001-5544-0431 AD - EcoHealth Alliance, New York, New York, USA. FAU - Daszak, Peter AU - Daszak P AD - EcoHealth Alliance, New York, New York, USA. FAU - Shi, Zheng-Li AU - Shi ZL AUID- ORCID: 0000-0001-8089-163X AD - Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China zlshi@wh.iov.cn. LA - eng GR - R01 AI110964/AI/NIAID NIH HHS/United States PT - Journal Article DEP - 20160624 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Antiviral Agents) RN - 0 (NF-kappa B) RN - 77238-31-4 (Interferon-beta) SB - IM MH - Animals MH - Antiviral Agents/pharmacology MH - Chiroptera/*virology MH - Chlorocebus aethiops MH - HeLa Cells MH - Humans MH - Interferon-beta/pharmacology MH - NF-kappa B/metabolism MH - Open Reading Frames/genetics/*immunology MH - Severe acute respiratory syndrome-related coronavirus/*immunology MH - Severe Acute Respiratory Syndrome/drug therapy/*immunology/virology MH - Vero Cells MH - Virus Replication/*immunology PMC - PMC4936131 EDAT- 2016/05/14 06:00 MHDA- 2017/05/19 06:00 PMCR- 2016/12/24 CRDT- 2016/05/13 06:00 PHST- 2015/12/09 00:00 [received] PHST- 2016/05/01 00:00 [accepted] PHST- 2016/05/13 06:00 [entrez] PHST- 2016/05/14 06:00 [pubmed] PHST- 2017/05/19 06:00 [medline] PHST- 2016/12/24 00:00 [pmc-release] AID - JVI.03079-15 [pii] AID - 03079-15 [pii] AID - 10.1128/JVI.03079-15 [doi] PST - epublish SO - J Virol. 2016 Jun 24;90(14):6573-6582. doi: 10.1128/JVI.03079-15. Print 2016 Jul 15. PMID- 25518718 OWN - NLM STAT- MEDLINE DCOM- 20150312 LR - 20191113 IS - 0001-723X (Print) IS - 0001-723X (Linking) VI - 58 IP - 4 DP - 2014 TI - Association of host tropism of Middle East syndrome coronavirus with the amino acid structure of host cell receptor dipeptidyl peptidase 4. PG - 359-63 AB - The Middle East syndrome coronavirus (MERS-CoV) is a recently emerging betacoronavirus with high fatality. Recently, dipeptidyle peptidase (CD26, DPP4) was identified as the host cell receptor for MERS-CoV. Interestingly, despite of common presence of DPP4 receptors the binding and infection of various cells shows imminent variability. In this report, we provide a tool for prediction of the host tropism of the virus based on the host receptor binding interface. We found out that, in the binding of MERS-CoV to cells the amino acid residues in lancets 4 and 5 of DPP4 receptor, namely K267, Q286, T288, R317, R336, Q344 A291, L294, and I295 are involved. Changes in these residues correspond to profound decrease in virus binding to cells. The nine residues at the interface between the virus spikes and the lancets 4 and 5 of host DPP4 can be used as a predictive tool for the host tropism and virus affinity to host cell receptors. FAU - Kandeel, M AU - Kandeel M FAU - Elaiziz, M A AU - Elaiziz MA FAU - Kandeel, A AU - Kandeel A FAU - Altaher, A A AU - Altaher AA FAU - Kitade, Y AU - Kitade Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Acta Virol JT - Acta virologica JID - 0370401 RN - 0 (Receptors, Virus) RN - EC 3.4.14.5 (Dipeptidyl Peptidase 4) SB - IM MH - Amino Acid Sequence MH - Animals MH - Coronavirus Infections/*enzymology/genetics/*veterinary/virology MH - Dipeptidyl Peptidase 4/*chemistry/genetics/metabolism MH - Humans MH - Mammals/genetics/virology MH - Middle East Respiratory Syndrome Coronavirus/classification/genetics/*physiology MH - Molecular Sequence Data MH - Receptors, Virus/*chemistry/genetics/metabolism MH - Sequence Alignment MH - *Viral Tropism EDAT- 2014/12/19 06:00 MHDA- 2015/03/13 06:00 CRDT- 2014/12/19 06:00 PHST- 2014/12/19 06:00 [entrez] PHST- 2014/12/19 06:00 [pubmed] PHST- 2015/03/13 06:00 [medline] AID - 10.4149/av_2014_04_359 [doi] PST - ppublish SO - Acta Virol. 2014;58(4):359-63. doi: 10.4149/av_2014_04_359. PMID- 28528587 OWN - NLM STAT- MEDLINE DCOM- 20170630 LR - 20220716 IS - 1469-4409 (Electronic) IS - 0950-2688 (Print) IS - 0950-2688 (Linking) VI - 145 IP - 10 DP - 2017 Jul TI - Persistent infections support maintenance of a coronavirus in a population of Australian bats (Myotis macropus). PG - 2053-2061 LID - 10.1017/S0950268817000991 [doi] AB - Understanding viral transmission dynamics within populations of reservoir hosts can facilitate greater knowledge of the spillover of emerging infectious diseases. While bat-borne viruses are of concern to public health, investigations into their dynamics have been limited by a lack of longitudinal data from individual bats. Here, we examine capture-mark-recapture (CMR) data from a species of Australian bat (Myotis macropus) infected with a putative novel Alphacoronavirus within a Bayesian framework. Then, we developed epidemic models to estimate the effect of persistently infectious individuals (which shed viruses for extensive periods) on the probability of viral maintenance within the study population. We found that the CMR data analysis supported grouping of infectious bats into persistently and transiently infectious bats. Maintenance of coronavirus within the study population was more likely in an epidemic model that included both persistently and transiently infectious bats, compared with the epidemic model with non-grouping of bats. These findings, using rare CMR data from longitudinal samples of individual bats, increase our understanding of transmission dynamics of bat viral infectious diseases. FAU - Jeong, J AU - Jeong J AD - Griffith Wildlife Disease Ecology Group, Environmental Futures Research Institute, School of Environment, Griffith University, Nathan, Queensland, Australia. FAU - Smith, C S AU - Smith CS AD - School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia. FAU - Peel, A J AU - Peel AJ AD - Griffith Wildlife Disease Ecology Group, Environmental Futures Research Institute, School of Environment, Griffith University, Nathan, Queensland, Australia. FAU - Plowright, R K AU - Plowright RK AD - Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA. FAU - Kerlin, D H AU - Kerlin DH AD - Griffith Wildlife Disease Ecology Group, Environmental Futures Research Institute, School of Environment, Griffith University, Nathan, Queensland, Australia. FAU - McBroom, J AU - McBroom J AD - School of Environment, Griffith University, Nathan, Queensland, Australia. FAU - McCallum, H AU - McCallum H AD - Griffith Wildlife Disease Ecology Group, Environmental Futures Research Institute, School of Environment, Griffith University, Nathan, Queensland, Australia. LA - eng GR - P20 GM103474/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170522 PL - England TA - Epidemiol Infect JT - Epidemiology and infection JID - 8703737 SB - IM MH - Animals MH - Australia/epidemiology MH - Bayes Theorem MH - *Chiroptera MH - Communicable Diseases, Emerging/epidemiology/*veterinary/virology MH - Coronavirus/*physiology MH - Coronavirus Infections/epidemiology/*veterinary/virology MH - Disease Reservoirs/virology MH - Models, Theoretical MH - Prevalence MH - Seroepidemiologic Studies PMC - PMC5776035 MID - NIHMS933497 OTO - NOTNLM OT - Myotis macropus OT - Bayesian analysis OT - coronavirus OT - epidemiological modelling OT - persistent infection COIS- None. EDAT- 2017/05/23 06:00 MHDA- 2017/07/01 06:00 PMCR- 2017/05/22 CRDT- 2017/05/23 06:00 PHST- 2017/05/23 06:00 [pubmed] PHST- 2017/07/01 06:00 [medline] PHST- 2017/05/23 06:00 [entrez] PHST- 2017/05/22 00:00 [pmc-release] AID - S0950268817000991 [pii] AID - 00099 [pii] AID - 10.1017/S0950268817000991 [doi] PST - ppublish SO - Epidemiol Infect. 2017 Jul;145(10):2053-2061. doi: 10.1017/S0950268817000991. Epub 2017 May 22. PMID- 28817732 OWN - NLM STAT- MEDLINE DCOM- 20171002 LR - 20181113 IS - 1553-7374 (Electronic) IS - 1553-7366 (Print) IS - 1553-7366 (Linking) VI - 13 IP - 8 DP - 2017 Aug TI - Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody. PG - e1006565 LID - 10.1371/journal.ppat.1006565 [doi] LID - e1006565 AB - The Middle East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic betacoronavirus that was first detected in humans in 2012 as a cause of severe acute respiratory disease. As of July 28, 2017, there have been 2,040 confirmed cases with 712 reported deaths. While many infections have been fatal, there have also been a large number of mild or asymptomatic cases discovered through monitoring and contact tracing. New Zealand white rabbits are a possible model for asymptomatic infection with MERS-CoV. In order to discover more about non-lethal infections and to learn whether a single infection with MERS-CoV would protect against reinfection, we inoculated rabbits with MERS-CoV and monitored the antibody and inflammatory response. Following intranasal infection, rabbits developed a transient dose-dependent pulmonary infection with moderately high levels of viral RNA, viral antigen, and perivascular inflammation in multiple lung lobes that was not associated with clinical signs. The rabbits developed antibodies against viral proteins that lacked neutralizing activity and the animals were not protected from reinfection. In fact, reinfection resulted in enhanced pulmonary inflammation, without an associated increase in viral RNA titers. Interestingly, passive transfer of serum from previously infected rabbits to naïve rabbits was associated with enhanced inflammation upon infection. We further found this inflammation was accompanied by increased recruitment of complement proteins compared to primary infection. However, reinfection elicited neutralizing antibodies that protected rabbits from subsequent viral challenge. Our data from the rabbit model suggests that people exposed to MERS-CoV who fail to develop a neutralizing antibody response, or persons whose neutralizing antibody titers have waned, may be at risk for severe lung disease on re-exposure to MERS-CoV. FAU - Houser, Katherine V AU - Houser KV AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Broadbent, Andrew J AU - Broadbent AJ AUID- ORCID: 0000-0002-4716-1835 AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Gretebeck, Lisa AU - Gretebeck L AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Vogel, Leatrice AU - Vogel L AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Lamirande, Elaine W AU - Lamirande EW AUID- ORCID: 0000-0002-3597-7319 AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Sutton, Troy AU - Sutton T AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Bock, Kevin W AU - Bock KW AUID- ORCID: 0000-0001-9454-0937 AD - Comparative Medicine Branch, Infectious Disease Pathogenesis Section, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Minai, Mahnaz AU - Minai M AUID- ORCID: 0000-0002-3642-8063 AD - Comparative Medicine Branch, Infectious Disease Pathogenesis Section, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Orandle, Marlene AU - Orandle M AD - Comparative Medicine Branch, Infectious Disease Pathogenesis Section, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Moore, Ian N AU - Moore IN AD - Comparative Medicine Branch, Infectious Disease Pathogenesis Section, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. FAU - Subbarao, Kanta AU - Subbarao K AUID- ORCID: 0000-0003-1713-3056 AD - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States of America. LA - eng PT - Journal Article DEP - 20170817 PL - United States TA - PLoS Pathog JT - PLoS pathogens JID - 101238921 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) SB - IM MH - Animals MH - Antibodies, Neutralizing/*immunology MH - Antibodies, Viral/*immunology MH - Coronavirus Infections/*immunology MH - Disease Models, Animal MH - Enzyme-Linked Immunosorbent Assay MH - Fluorescent Antibody Technique MH - Immunohistochemistry MH - Inflammation/immunology MH - Middle East Respiratory Syndrome Coronavirus/immunology MH - Neutralization Tests MH - Polymerase Chain Reaction MH - Rabbits PMC - PMC5574614 COIS- The authors have declared that no competing interests exist EDAT- 2017/08/18 06:00 MHDA- 2017/10/03 06:00 PMCR- 2017/08/17 CRDT- 2017/08/18 06:00 PHST- 2017/02/17 00:00 [received] PHST- 2017/08/01 00:00 [accepted] PHST- 2017/08/29 00:00 [revised] PHST- 2017/08/18 06:00 [pubmed] PHST- 2017/10/03 06:00 [medline] PHST- 2017/08/18 06:00 [entrez] PHST- 2017/08/17 00:00 [pmc-release] AID - PPATHOGENS-D-17-00350 [pii] AID - 10.1371/journal.ppat.1006565 [doi] PST - epublish SO - PLoS Pathog. 2017 Aug 17;13(8):e1006565. doi: 10.1371/journal.ppat.1006565. eCollection 2017 Aug. PMID- 30644842 OWN - NLM STAT- MEDLINE DCOM- 20190520 LR - 20241212 IS - 2059-7983 (Electronic) IS - 2059-7983 (Linking) VI - 75 IP - Pt 1 DP - 2019 Jan 1 TI - Structure of interferon-stimulated gene product 15 (ISG15) from the bat species Myotis davidii and the impact of interdomain ISG15 interactions on viral protein engagement. PG - 21-31 LID - 10.1107/S2059798318015322 [doi] AB - Bats have long been observed to be the hosts and the origin of numerous human diseases. Bats, like all mammals, rely on a number of innate immune mechanisms to combat invading pathogens, including the interferon type I, II and III responses. Ubiquitin-like interferon-stimulated gene product 15 (ISG15) is a key modulator of these interferon responses. Within these pathways, ISG15 can serve to stabilize host proteins modulating innate immune responses and act as a cytokine. Post-translational modifications of viral proteins introduced by ISG15 have also been observed to directly affect the function of numerous viral proteins. Unlike ubiquitin, which is virtually identical across all animals, comparison of ISG15s across species reveals that they are relatively divergent, with sequence identity dropping to as low as ∼58% among mammals. In addition to serving as an obstacle to the zoonotic transmission of influenza, these ISG15 species-species differences have also long been shown to have an impact on the function of viral deISGylases. Recently, the structure of the first nonhuman ISG15, originating from mouse, suggested that the structures of human ISG15 may not be reflective of other species. Here, the structure of ISG15 from the bat species Myotis davidii solved to 1.37 Å resolution is reported. Comparison of this ISG15 structure with those from human and mouse not only underscores the structural impact of ISG15 species-species differences, but also highlights a conserved hydrophobic motif formed between the two domains of ISG15. Using the papain-like deISGylase from Severe acute respiratory syndrome coronavirus as a probe, the biochemical importance of this motif in ISG15-protein engagements was illuminated. FAU - Langley, Caroline AU - Langley C AD - Pharmaceutical and Biomedical Sciences, University of Georgia, 240 West Green Street, Athens, GA 30602, USA. FAU - Goodwin, Octavia AU - Goodwin O AD - Pharmaceutical and Biomedical Sciences, University of Georgia, 240 West Green Street, Athens, GA 30602, USA. FAU - Dzimianski, John V AU - Dzimianski JV AD - Pharmaceutical and Biomedical Sciences, University of Georgia, 240 West Green Street, Athens, GA 30602, USA. FAU - Daczkowski, Courtney M AU - Daczkowski CM AD - Pharmaceutical and Biomedical Sciences, University of Georgia, 240 West Green Street, Athens, GA 30602, USA. FAU - Pegan, Scott D AU - Pegan SD AUID- ORCID: 0000-0002-2958-5319 AD - Pharmaceutical and Biomedical Sciences, University of Georgia, 240 West Green Street, Athens, GA 30602, USA. LA - eng GR - R01 AI109008/AI/NIAID NIH HHS/United States GR - AI109008/National Institute of Allergy and Infectious Diseases/ GR - 58-5030-5-034/Agricultural Research Service/ PT - Journal Article DEP - 20190104 PL - United States TA - Acta Crystallogr D Struct Biol JT - Acta crystallographica. Section D, Structural biology JID - 101676043 RN - 0 (Cytokines) RN - 0 (G1p2 protein, mouse) RN - 0 (Ubiquitins) RN - 0 (Viral Proteins) RN - 60267-61-0 (ISG15 protein, human) RN - 9008-11-1 (Interferons) SB - IM MH - Animals MH - Chiroptera MH - Crystallography, X-Ray MH - Cytokines/immunology MH - Humans MH - Interferons/immunology MH - Mice MH - Protein Domains MH - Protein Processing, Post-Translational MH - Ubiquitins/*chemistry MH - Viral Proteins/*metabolism PMC - PMC6333284 OTO - NOTNLM OT - ISG15 OT - Myotis davidii OT - coronaviruses OT - interferons OT - papain-like proteases OT - severe acute respiratory syndrome EDAT- 2019/01/16 06:00 MHDA- 2019/05/21 06:00 PMCR- 2020/01/01 CRDT- 2019/01/16 06:00 PHST- 2018/09/09 00:00 [received] PHST- 2018/10/30 00:00 [accepted] PHST- 2019/01/16 06:00 [entrez] PHST- 2019/01/16 06:00 [pubmed] PHST- 2019/05/21 06:00 [medline] PHST- 2020/01/01 00:00 [pmc-release] AID - S2059798318015322 [pii] AID - jb5006 [pii] AID - 10.1107/S2059798318015322 [doi] PST - ppublish SO - Acta Crystallogr D Struct Biol. 2019 Jan 1;75(Pt 1):21-31. doi: 10.1107/S2059798318015322. Epub 2019 Jan 4. PMID- 26536463 OWN - NLM STAT- MEDLINE DCOM- 20160404 LR - 20161126 IS - 1560-7917 (Electronic) IS - 1025-496X (Linking) VI - 20 IP - 37 DP - 2015 TI - Presence of antibodies but no evidence for circulation of MERS-CoV in dromedaries on the Canary Islands, 2015. LID - 10.2807/1560-7917.ES.2015.20.37.30019 [doi] AB - In 2012, a new betacoronavirus, Middle East respiratory syndrome coronavirus (MERS-CoV), was identified in humans. Several studies confirmed dromedary camels to be a potential reservoir and a source for human infection. Camels located on the Canary Islands were included in those studies and ca 10% of them were positive for MERS-CoV-specific antibodies. However, these findings could not be correctly interpreted because epidemiological information was not provided. Thus, further investigations were necessary to clarify these results. A total of 170 camels were investigated in this survey, of which seven (4.1%) were seropositive by ELISA. Epidemiological information revealed that all seropositive camels had been imported from Africa 20 or more years prior. We conclude that seropositive camels had contact with MERS-CoV in Africa and that there is no shedding of the virus among camels or people around the farms on the Canary Islands. However, the presence of antibodies in the camel herds should be monitored. FAU - Gutiérrez, Carlos AU - Gutiérrez C AD - Research Institute of Biomedical and Health Sciences, University of Las Palmas de Gran Canaria, Canary Islands, Spain. FAU - Tejedor-Junco, María Teresa AU - Tejedor-Junco MT FAU - González, Margarita AU - González M FAU - Lattwein, Erik AU - Lattwein E FAU - Renneker, Stefanie AU - Renneker S LA - eng PT - Journal Article PL - Sweden TA - Euro Surveill JT - Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin JID - 100887452 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (Immunoglobulin G) SB - IM MH - Animals MH - Antibodies, Neutralizing/blood MH - Antibodies, Viral/*blood MH - Camelus/blood/*virology MH - Coronavirus/immunology/*isolation & purification MH - Coronavirus Infections/*diagnosis/virology MH - Disease Reservoirs/virology MH - Enzyme-Linked Immunosorbent Assay MH - Humans MH - Immunoglobulin G/blood MH - Middle East Respiratory Syndrome Coronavirus/*isolation & purification MH - Prevalence MH - Spain/epidemiology OTO - NOTNLM OT - Canary Islands OT - MERS-Co OT - camels OT - seroprevalence EDAT- 2015/11/05 06:00 MHDA- 2016/04/05 06:00 CRDT- 2015/11/05 06:00 PHST- 2015/05/09 00:00 [received] PHST- 2015/08/13 00:00 [accepted] PHST- 2015/11/05 06:00 [entrez] PHST- 2015/11/05 06:00 [pubmed] PHST- 2016/04/05 06:00 [medline] AID - 30019 [pii] AID - 10.2807/1560-7917.ES.2015.20.37.30019 [doi] PST - ppublish SO - Euro Surveill. 2015;20(37). doi: 10.2807/1560-7917.ES.2015.20.37.30019. PMID- 26081714 OWN - NLM STAT- MEDLINE DCOM- 20151114 LR - 20190318 IS - 0253-9624 (Print) IS - 0253-9624 (Linking) VI - 49 IP - 5 DP - 2015 May TI - [Advances in the researches of genomic characterization and molecular detection of Middel East respiratory syndrome coronavirus]. PG - 461-4 AB - Middle east respiratory syndrome coronavirus (MERS-CoV) was recently identified as a novel human coronavirus known to infect human with high mortality. It belongs to C clade of the betacoronavirus shown the similar genomic structure as other human coronaviruses.To date, some different subtypes of the viral genome were identified but its origin was unclear. Some evidences indicated it maybe came from the bats or dromedary. And series of molecular detection methods have been established and applied in lab and clinic. FAU - Zhao, Yanjie AU - Zhao Y AD - Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325035, China. FAU - Tan, Wenjie AU - Tan W AD - Institute of Medical Virology, Wenzhou Medical University, Wenzhou 325035, China; Email: tanwj28@163.com. LA - chi PT - Journal Article PL - China TA - Zhonghua Yu Fang Yi Xue Za Zhi JT - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] JID - 7904962 SB - IM MH - Animals MH - Camelus MH - Chiroptera MH - Coronavirus MH - *Coronavirus Infections MH - *Genome, Viral MH - Genomics MH - Humans MH - *Middle East Respiratory Syndrome Coronavirus EDAT- 2015/06/18 06:00 MHDA- 2015/11/15 06:00 CRDT- 2015/06/18 06:00 PHST- 2015/06/18 06:00 [entrez] PHST- 2015/06/18 06:00 [pubmed] PHST- 2015/11/15 06:00 [medline] PST - ppublish SO - Zhonghua Yu Fang Yi Xue Za Zhi. 2015 May;49(5):461-4.